Restriction map for
pF1KB9977
(Circular) (Six-base) MAPSORT of: pF1KB9977.seq Check: 581 from: 1 to: 4784
pF1KB9977 4784 bp
With 182 enzymes: SgfI *
April 24, 2009 13:09 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 172 172
Size: 4784
AccI GT'mk_AC
Cuts at: 221 1822 221
Size: 1601 3183
Acc65I G'GTAC_C
Cuts at: 1800 1800
Size: 4784
AclI AA'CG_TT
Cuts at: 995 4624 995
Size: 3629 1155
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 539 611 1085 2609 3041 3788 4056 539
Size: 72 474 1524 432 747 268 1267
Fragments arranged by size:
1524 1267 747 474 432 268 72
AflIII A'CryG_T
Cuts at: 2136 3240 4217 2136
Size: 1104 977 2703
Fragments arranged by size:
2703 1104 977
AgeI A'CCGG_T
Cuts at: 3172 3172
Size: 4784
AleI CACnn'nnGTG
Cuts at: 1186 1186
Size: 4784
AlwNI CAG_nnn'CTG
Cuts at: 2089 3656 2089
Size: 1567 3217
ApaLI G'TGCA_C
Cuts at: 3554 3554
Size: 4784
ApoI r'AATT_y
Cuts at: 671 1010 1415 1433 1788 4137 4393 671
Size: 339 405 18 355 2349 256 1062
Fragments arranged by size:
2349 1062 405 355 339 256 18
AseI AT'TA_AT
Cuts at: 20 20
Size: 4784
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 4784
AvaI C'yCGr_G
Cuts at: 1804 1804
Size: 4784
BamHI G'GATC_C
Cuts at: 1809 1809
Size: 4784
BanI G'GyrC_C
Cuts at: 156 1800 2214 2462 2497 156
Size: 1644 414 248 35 2443
Fragments arranged by size:
2443 1644 414 248 35
BanII G_rGCy'C
Cuts at: 1798 2828 1798
Size: 1030 3754
BbeI G_GCGC'C
Cuts at: 160 2218 2466 160
Size: 2058 248 2478
Fragments arranged by size:
2478 2058 248
BbsI GAAGACnn'nnnn_
Cuts at: 1476 1500 1476
Size: 24 4760
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 123 157 123
Size: 34 4750
BciVI GTATCCnnnnn_n'
Cuts at: 2682 3443 4784 2682
Size: 761 1341 2682
Fragments arranged by size:
2682 1341 761
BclI T'GATC_A
Cuts at: 2304 2304
Size: 4784
BfrBI ATG'CAT
Cuts at: 1496 1496
Size: 4784
BglII A'GATC_T
Cuts at: 2299 4336 2299
Size: 2037 2747
BlpI GC'TnA_GC
Cuts at: 1896 1896
Size: 4784
Bme1580I G_kGCm'C
Cuts at: 2409 2502 3558 2409
Size: 93 1056 3635
Fragments arranged by size:
3635 1056 93
BmrI ACTGGGnnnn_n'
Cuts at: 320 969 1996 2077 2412 4556 320
Size: 649 1027 81 335 2144 548
Fragments arranged by size:
2144 1027 649 548 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 477 1569 3151 477
Size: 1092 1582 2110
Fragments arranged by size:
2110 1582 1092
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 482 1094 482
Size: 612 4172
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 367 1509 1960 2839 3331 3629 367
Size: 1142 451 879 492 298 1522
Fragments arranged by size:
1522 1142 879 492 451 298
BsaI GGTCTCn'nnnn_
Cuts at: 4488 4488
Size: 4784
BsaAI yAC'GTr
Cuts at: 2137 2137
Size: 4784
BsaBI GATnn'nnATC
Cuts at: 6 541 1673 2298 4335 6
Size: 535 1132 625 2037 455
Fragments arranged by size:
2037 1132 625 535 455
BsaHI Gr'CG_yC
Cuts at: 157 2215 2463 3165 4661 157
Size: 2058 248 702 1496 280
Fragments arranged by size:
2058 1496 702 280 248
BsaWI w'CCGG_w
Cuts at: 1973 2494 3172 3446 3593 4004 1973
Size: 521 678 274 147 411 2753
Fragments arranged by size:
2753 678 521 411 274 147
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 194 194
Size: 4784
BseYI C'CCAG_C
Cuts at: 2209 2967 3544 4040 2209
Size: 758 577 496 2953
Fragments arranged by size:
2953 758 577 496
BsiEI CG_ry'CG
Cuts at: 104 2326 2372 3580 104
Size: 2222 46 1208 1308
Fragments arranged by size:
2222 1308 1208 46
BsiHKAI G_wGCw'C
Cuts at: 146 1164 1332 1798 2576 2766 3558 146
Size: 1018 168 466 778 190 792 1372
Fragments arranged by size:
1372 1018 792 778 466 190 168
Bsp1286I G_dGCh'C
Cuts at: 146 1164 1332 1798 2409 2502 2576 2766 2828
Size: 1018 168 466 611 93 74 190 62
Cuts at: 2828 3558 146
Size: 730 1372
Fragments arranged by size:
1372 1018 730 611 466 190 168 93
74 62
BspEI T'CCGG_A
Cuts at: 4004 4004
Size: 4784
BspHI T'CATG_A
Cuts at: 3960 3960
Size: 4784
BspMI ACCTGCnnnn'nnnn_
Cuts at: 172 1834 2048 2350 2731 172
Size: 1662 214 302 381 2225
Fragments arranged by size:
2225 1662 381 302 214
BsrBI CCG'CTC
Cuts at: 50 3076 3130 4614 4779 50
Size: 3026 54 1484 165 55
Fragments arranged by size:
3026 1484 165 55 54
BsrDI GCAATG_nn'
Cuts at: 2696 2696
Size: 4784
BsrFI r'CCGG_y
Cuts at: 2782 2963 3172 2782
Size: 181 209 4394
Fragments arranged by size:
4394 209 181
BssSI C'ACGA_G
Cuts at: 3055 3413 3055
Size: 358 4426
BstAPI GCAn_nnn'nTGC
Cuts at: 1450 2161 1450
Size: 711 4073
BstBI TT'CG_AA
Cuts at: 3145 3145
Size: 4784
BstYI r'GATC_y
Cuts at: 1 1126 1809 2276 2299 2634 2880 3892 3978
Size: 1125 683 467 23 335 246 1012 86
Cuts at: 3978 3990 4336 1
Size: 12 346 449
Fragments arranged by size:
1125 1012 683 467 449 346 335 246
86 23 12
Bsu36I CC'TnA_GG
Cuts at: 247 247
Size: 4784
BtgI C'CryG_G
Cuts at: 152 160 1337 152
Size: 8 1177 3599
Fragments arranged by size:
3599 1177 8
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 278 2734 2915 3078 278
Size: 2456 181 163 1984
Fragments arranged by size:
2456 1984 181 163
BtsI GCAGTG_nn'
Cuts at: 1368 2027 1368
Size: 659 4125
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 868 903 868
Size: 35 4749
DraI TTT'AAA
Cuts at: 905 1783 905
Size: 878 3906
DrdI GACnn_nn'nnGTC
Cuts at: 2490 3348 2490
Size: 858 3926
EaeI y'GGCC_r
Cuts at: 2369 2543 2934 2961 4306 2369
Size: 174 391 27 1345 2847
Fragments arranged by size:
2847 1345 391 174 27
EagI C'GGCC_G
Cuts at: 2369 2369
Size: 4784
EarI CTCTTCn'nnn_
Cuts at: 630 1061 2807 3017 630
Size: 431 1746 210 2397
Fragments arranged by size:
2397 1746 431 210
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 2992 3302 3448 4594 2992
Size: 310 146 1146 3182
Fragments arranged by size:
3182 1146 310 146
EcoICRI GAG'CTC
Cuts at: 1796 1796
Size: 4784
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 482 539 611 1085 1094 2609 3041 3788 4056
Size: 57 72 474 9 1515 432 747 268
Cuts at: 4056 482
Size: 1210
Fragments arranged by size:
1515 1210 747 474 432 268 72 57
9
EcoO109I rG'GnC_Cy
Cuts at: 1923 1923
Size: 4784
EcoRI G'AATT_C
Cuts at: 1788 1788
Size: 4784
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2803 2803
Size: 4784
FspI TGC'GCA
Cuts at: 2565 2565
Size: 4784
HaeII r_GCGC'y
Cuts at: 160 2218 2466 3488 4467 160
Size: 2058 248 1022 979 477
Fragments arranged by size:
2058 1022 979 477 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 480 512 2626 2656 2658 2688 480
Size: 32 2114 30 2 30 2576
Fragments arranged by size:
2576 2114 32 30 30 2
HincII GTy'rAC
Cuts at: 222 459 1823 222
Size: 237 1364 3183
Fragments arranged by size:
3183 1364 237
HindIII A'AGCT_T
Cuts at: 591 1094 591
Size: 503 4281
KasI G'GCGC_C
Cuts at: 156 2214 2462 156
Size: 2058 248 2478
Fragments arranged by size:
2478 2058 248
KpnI G_GTAC'C
Cuts at: 1804 1804
Size: 4784
MfeI C'AATT_G
Cuts at: 1637 1637
Size: 4784
MluI A'CGCG_T
Cuts at: 4217 4217
Size: 4784
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1410 3455 3639 1410
Size: 2045 184 2555
Fragments arranged by size:
2555 2045 184
MscI TGG'CCA
Cuts at: 2545 2545
Size: 4784
MslI CAynn'nnrTG
Cuts at: 1186 2773 2900 1186
Size: 1587 127 3070
Fragments arranged by size:
3070 1587 127
MspA1I CmG'CkG
Cuts at: 1895 2209 2569 3582 3827 1895
Size: 314 360 1013 245 2852
Fragments arranged by size:
2852 1013 360 314 245
NaeI GCC'GGC
Cuts at: 2965 2965
Size: 4784
NarI GG'CG_CC
Cuts at: 157 2215 2463 157
Size: 2058 248 2478
Fragments arranged by size:
2478 2058 248
NcoI C'CATG_G
Cuts at: 160 1337 160
Size: 1177 3607
NdeI CA'TA_TG
Cuts at: 861 861
Size: 4784
NgoMIV G'CCGG_C
Cuts at: 2963 2963
Size: 4784
NsiI A_TGCA'T
Cuts at: 1498 1498
Size: 4784
NspI r_CATG'y
Cuts at: 1837 2337 3244 1837
Size: 500 907 3377
Fragments arranged by size:
3377 907 500
PciI A'CATG_T
Cuts at: 3240 3240
Size: 4784
PfoI T'CCnGG_A
Cuts at: 549 915 1193 549
Size: 366 278 4140
Fragments arranged by size:
4140 366 278
PmeI GTTT'AAAC
Cuts at: 1783 1783
Size: 4784
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3923 3955 3923
Size: 32 4752
PsiI TTA'TAA
Cuts at: 677 677
Size: 4784
PstI C_TGCA'G
Cuts at: 1831 2516 1831
Size: 685 4099
PvuI CG_AT'CG
Cuts at: 104 104
Size: 4784
PvuII CAG'CTG
Cuts at: 2209 2569 2209
Size: 360 4424
RsrII CG'GwC_CG
Cuts at: 2979 2979
Size: 4784
SacI G_AGCT'C
Cuts at: 1798 1798
Size: 4784
SalI G'TCGA_C
Cuts at: 220 1821 220
Size: 1601 3183
SapI GCTCTTCn'nnn_
Cuts at: 2807 3017 2807
Size: 210 4574
SbfI CC_TGCA'GG
Cuts at: 1831 1831
Size: 4784
ScaI AGT'ACT
Cuts at: 4301 4301
Size: 4784
SfcI C'TryA_G
Cuts at: 33 750 1643 1762 1827 2512 3505 3696 33
Size: 717 893 119 65 685 993 191 1121
Fragments arranged by size:
1121 993 893 717 685 191 119 65
SfoI GGC'GCC
Cuts at: 158 2216 2464 158
Size: 2058 248 2478
Fragments arranged by size:
2478 2058 248
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 4784
SmaI CCC'GGG
Cuts at: 1806 1806
Size: 4784
SmlI C'TyrA_G
Cuts at: 382 1488 1939 2854 3346 3608 382
Size: 1106 451 915 492 262 1558
Fragments arranged by size:
1558 1106 915 492 451 262
SpeI A'CTAG_T
Cuts at: 67 355 67
Size: 288 4496
SphI G_CATG'C
Cuts at: 1837 2337 1837
Size: 500 4284
SspI AAT'ATT
Cuts at: 351 654 351
Size: 303 4481
StyI C'CwwG_G
Cuts at: 160 1337 1918 2272 160
Size: 1177 581 354 2672
Fragments arranged by size:
2672 1177 581 354
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 153 329 2367 2500 2959 3168 153
Size: 176 2038 133 459 209 1769
Fragments arranged by size:
2038 1769 459 209 176 133
TatI w'GTAC_w
Cuts at: 504 759 4299 504
Size: 255 3540 989
Fragments arranged by size:
3540 989 255
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 729 761 4301 4333 729
Size: 32 3540 32 1180
Fragments arranged by size:
3540 1180 32 32
Tth111I GACn'n_nGTC
Cuts at: 2581 2581
Size: 4784
XbaI T'CTAG_A
Cuts at: 1815 1815
Size: 4784
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2279 2279
Size: 4784
XmaI C'CCGG_G
Cuts at: 1804 1804
Size: 4784
XmnI GAAnn'nnTTC
Cuts at: 1483 1719 1483
Size: 236 4548
Enzymes that do cut:
AarI AccI Acc65I AclI AcuI AflIII AgeI AleI
AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI BanI
BanII BbeI BbsI BcgI BciVI BclI BfrBI BglII
BlpI Bme1580I BmrI BplI BpmI BpuEI BsaI BsaAI
BsaBI BsaHI BsaWI BseRI BseYI BsiEI BsiHKAI Bsp1286I
BspEI BspHI BspMI BsrBI BsrDI BsrFI BssSI BstAPI
BstBI BstYI Bsu36I BtgI BtgZI BtsI CspCI DraI
DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoO109I
EcoRI FalI FspI HaeII Hin4I HincII HindIII KasI
KpnI MfeI MluI MmeI MscI MslI MspA1I NaeI
NarI NcoI NdeI NgoMIV NsiI NspI PciI PfoI
PmeI PpiI PsiI PstI PvuI PvuII RsrII SacI
SalI SapI SbfI ScaI SfcI SfoI SgfI SmaI
SmlI SpeI SphI SspI StyI TaqII TatI TstI
Tth111I XbaI XcmI XmaI XmnI
Enzymes that do not cut:
AatII AfeI AflII AhdI AlfI AloI ApaI AscI
AvrII BaeI BbvCI BglI BmgBI BmtI Bpu10I BsaXI
BsgI BsiWI BsmI BsmBI BsrGI BssHII BstEII BstXI
BstZ17I ClaI DraIII EcoNI EcoRV FseI FspAI HpaI
NheI NotI NruI PacI PasI PflMI PmlI PpuMI
PshAI PspOMI PspXI PsrI SacII SanDI SexAI SfiI
SgrAI SnaBI SrfI StuI SwaI XhoI ZraI