Restriction map for
pF1KB9980
(Circular) (Six-base) MAPSORT of: pF1KB9980.seq Check: 1171 from: 1 to: 5237
pF1KB9980 5237 bp
With 182 enzymes: SgfI *
October 7, 2009 14:27 ..
AccI GT'mk_AC
Cuts at: 2275 2275
Size: 5237
Acc65I G'GTAC_C
Cuts at: 2253 2253
Size: 5237
AclI AA'CG_TT
Cuts at: 5077 5077
Size: 5237
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1355 3062 3494 4241 4509 1355
Size: 1707 432 747 268 2083
Fragments arranged by size:
2083 1707 747 432 268
AflIII A'CryG_T
Cuts at: 954 2589 3693 4670 954
Size: 1635 1104 977 1521
Fragments arranged by size:
1635 1521 1104 977
AgeI A'CCGG_T
Cuts at: 3625 3625
Size: 5237
AlwNI CAG_nnn'CTG
Cuts at: 1897 2542 4109 1897
Size: 645 1567 3025
Fragments arranged by size:
3025 1567 645
ApaLI G'TGCA_C
Cuts at: 4007 4007
Size: 5237
ApoI r'AATT_y
Cuts at: 233 383 1001 1258 1486 1854 2241 4590 4846
Size: 150 618 257 228 368 387 2349 256
Cuts at: 4846 233
Size: 624
Fragments arranged by size:
2349 624 618 387 368 257 256 228
150
AseI AT'TA_AT
Cuts at: 20 20
Size: 5237
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 5237
AvaI C'yCGr_G
Cuts at: 334 2257 334
Size: 1923 3314
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'
Cuts at: 203 236 203
Size: 33 5204
BamHI G'GATC_C
Cuts at: 652 2262 652
Size: 1610 3627
BanI G'GyrC_C
Cuts at: 2253 2667 2915 2950 2253
Size: 414 248 35 4540
Fragments arranged by size:
4540 414 248 35
BanII G_rGCy'C
Cuts at: 2251 3281 2251
Size: 1030 4207
BbeI G_GCGC'C
Cuts at: 2671 2919 2671
Size: 248 4989
BbsI GAAGACnn'nnnn_
Cuts at: 1595 2072 1595
Size: 477 4760
BciVI GTATCCnnnnn_n'
Cuts at: 3135 3896 5237 3135
Size: 761 1341 3135
Fragments arranged by size:
3135 1341 761
BclI T'GATC_A
Cuts at: 393 2757 393
Size: 2364 2873
BfrBI ATG'CAT
Cuts at: 996 996
Size: 5237
BglII A'GATC_T
Cuts at: 1398 1696 2752 4789 1398
Size: 298 1056 2037 1846
Fragments arranged by size:
2037 1846 1056 298
BlpI GC'TnA_GC
Cuts at: 2349 2349
Size: 5237
Bme1580I G_kGCm'C
Cuts at: 2862 2955 4011 2862
Size: 93 1056 4088
Fragments arranged by size:
4088 1056 93
BmrI ACTGGGnnnn_n'
Cuts at: 2449 2530 2865 5009 2449
Size: 81 335 2144 2677
Fragments arranged by size:
2677 2144 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3604 3604
Size: 5237
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1924 1924
Size: 5237
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 294 2413 3292 3784 4082 294
Size: 2119 879 492 298 1449
Fragments arranged by size:
2119 1449 879 492 298
BsaI GGTCTCn'nnnn_
Cuts at: 705 1880 4941 705
Size: 1175 3061 1001
Fragments arranged by size:
3061 1175 1001
BsaAI yAC'GTr
Cuts at: 2590 2590
Size: 5237
BsaBI GATnn'nnATC
Cuts at: 6 170 2189 2751 4788 6
Size: 164 2019 562 2037 455
Fragments arranged by size:
2037 2019 562 455 164
BsaHI Gr'CG_yC
Cuts at: 2668 2916 3618 5114 2668
Size: 248 702 1496 2791
Fragments arranged by size:
2791 1496 702 248
BsaWI w'CCGG_w
Cuts at: 603 2426 2947 3625 3899 4046 4457 603
Size: 1823 521 678 274 147 411 1383
Fragments arranged by size:
1823 1383 678 521 411 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 929 959 929
Size: 30 5207
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1516 1618 1723 1883 1516
Size: 102 105 160 4870
Fragments arranged by size:
4870 160 105 102
BseYI C'CCAG_C
Cuts at: 2662 3420 3997 4493 2662
Size: 758 577 496 3406
Fragments arranged by size:
3406 758 577 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1459 1459
Size: 5237
BsiEI CG_ry'CG
Cuts at: 104 2779 2825 4033 104
Size: 2675 46 1208 1308
Fragments arranged by size:
2675 1308 1208 46
BsiHKAI G_wGCw'C
Cuts at: 1692 2251 3029 3219 4011 1692
Size: 559 778 190 792 2918
Fragments arranged by size:
2918 792 778 559 190
Bsp1286I G_dGCh'C
Cuts at: 1692 2251 2862 2955 3029 3219 3281 4011 1692
Size: 559 611 93 74 190 62 730 2918
Fragments arranged by size:
2918 730 611 559 190 93 74 62
BspEI T'CCGG_A
Cuts at: 4457 4457
Size: 5237
BspHI T'CATG_A
Cuts at: 294 492 4413 294
Size: 198 3921 1118
Fragments arranged by size:
3921 1118 198
BspMI ACCTGCnnnn'nnnn_
Cuts at: 337 2287 2501 2803 3184 337
Size: 1950 214 302 381 2390
Fragments arranged by size:
2390 1950 381 302 214
BsrBI CCG'CTC
Cuts at: 50 3529 3583 5067 5232 50
Size: 3479 54 1484 165 55
Fragments arranged by size:
3479 1484 165 55 54
BsrDI GCAATG_nn'
Cuts at: 3149 3149
Size: 5237
BsrFI r'CCGG_y
Cuts at: 3235 3416 3625 3235
Size: 181 209 4847
Fragments arranged by size:
4847 209 181
BssSI C'ACGA_G
Cuts at: 3508 3866 3508
Size: 358 4879
BstAPI GCAn_nnn'nTGC
Cuts at: 523 2614 523
Size: 2091 3146
BstBI TT'CG_AA
Cuts at: 3598 3598
Size: 5237
BstYI r'GATC_y
Cuts at: 1 652 1398 1696 2262 2729 2752 3087 3333
Size: 651 746 298 566 467 23 335 246
Cuts at: 3333 4345 4431 4443 4789 1
Size: 1012 86 12 346 449
Fragments arranged by size:
1012 746 651 566 467 449 346 335
298 246 86 23 12
BtgI C'CryG_G
Cuts at: 427 427
Size: 5237
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 3187 3368 3531 3187
Size: 181 163 4893
Fragments arranged by size:
4893 181 163
BtsI GCAGTG_nn'
Cuts at: 1447 2480 1447
Size: 1033 4204
ClaI AT'CG_AT
Cuts at: 1598 1598
Size: 5237
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 563 598 563
Size: 35 5202
DraI TTT'AAA
Cuts at: 147 1980 2236 147
Size: 1833 256 3148
Fragments arranged by size:
3148 1833 256
DraIII CAC_nnn'GTG
Cuts at: 523 1017 523
Size: 494 4743
DrdI GACnn_nn'nnGTC
Cuts at: 1941 2943 3801 1941
Size: 1002 858 3377
Fragments arranged by size:
3377 1002 858
EaeI y'GGCC_r
Cuts at: 2822 2996 3387 3414 4759 2822
Size: 174 391 27 1345 3300
Fragments arranged by size:
3300 1345 391 174 27
EagI C'GGCC_G
Cuts at: 2822 2822
Size: 5237
EarI CTCTTCn'nnn_
Cuts at: 1013 1331 1809 1821 1830 1839 1881 2223 3260
Size: 318 478 12 9 9 42 342 1037
Cuts at: 3260 3470 1013
Size: 210 2780
Fragments arranged by size:
2780 1037 478 342 318 210 42 12
9 9
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 3445 3755 3901 5047 3445
Size: 310 146 1146 3635
Fragments arranged by size:
3635 1146 310 146
EcoICRI GAG'CTC
Cuts at: 2249 2249
Size: 5237
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1355 1924 3062 3494 4241 4509 1355
Size: 569 1138 432 747 268 2083
Fragments arranged by size:
2083 1138 747 569 432 268
EcoNI CCTnn'n_nnAGG
Cuts at: 1373 1373
Size: 5237
EcoO109I rG'GnC_Cy
Cuts at: 2376 2376
Size: 5237
EcoRI G'AATT_C
Cuts at: 1854 2241 1854
Size: 387 4850
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 1153 3256 1153
Size: 2103 3134
FspI TGC'GCA
Cuts at: 3018 3018
Size: 5237
HaeII r_GCGC'y
Cuts at: 2671 2919 3941 4920 2671
Size: 248 1022 979 2988
Fragments arranged by size:
2988 1022 979 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 715 747 1325 1357 1777 1809 2152 2184 3079
Size: 32 578 32 420 32 343 32 895
Cuts at: 3079 3109 3111 3141 715
Size: 30 2 30 2811
Fragments arranged by size:
2811 895 578 420 343 32 32 32
32 30 30 2
HincII GTy'rAC
Cuts at: 405 2276 405
Size: 1871 3366
HindIII A'AGCT_T
Cuts at: 1107 1107
Size: 5237
KasI G'GCGC_C
Cuts at: 2667 2915 2667
Size: 248 4989
KpnI G_GTAC'C
Cuts at: 2257 2257
Size: 5237
MluI A'CGCG_T
Cuts at: 4670 4670
Size: 5237
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 889 1520 3908 4092 889
Size: 631 2388 184 2034
Fragments arranged by size:
2388 2034 631 184
MscI TGG'CCA
Cuts at: 2998 2998
Size: 5237
MslI CAynn'nnrTG
Cuts at: 845 1577 3226 3353 845
Size: 732 1649 127 2729
Fragments arranged by size:
2729 1649 732 127
MspA1I CmG'CkG
Cuts at: 885 2348 2662 3022 4035 4280 885
Size: 1463 314 360 1013 245 1842
Fragments arranged by size:
1842 1463 1013 360 314 245
NaeI GCC'GGC
Cuts at: 3418 3418
Size: 5237
NarI GG'CG_CC
Cuts at: 2668 2916 2668
Size: 248 4989
NcoI C'CATG_G
Cuts at: 427 427
Size: 5237
NgoMIV G'CCGG_C
Cuts at: 3416 3416
Size: 5237
NsiI A_TGCA'T
Cuts at: 998 998
Size: 5237
NspI r_CATG'y
Cuts at: 958 2290 2790 3697 958
Size: 1332 500 907 2498
Fragments arranged by size:
2498 1332 907 500
PciI A'CATG_T
Cuts at: 954 3693 954
Size: 2739 2498
PmeI GTTT'AAAC
Cuts at: 2236 2236
Size: 5237
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4376 4408 4376
Size: 32 5205
PshAI GACnn'nnGTC
Cuts at: 599 1898 599
Size: 1299 3938
PspXI vC'TCGA_Gb
Cuts at: 334 334
Size: 5237
PstI C_TGCA'G
Cuts at: 120 2284 2969 120
Size: 2164 685 2388
Fragments arranged by size:
2388 2164 685
PvuI CG_AT'CG
Cuts at: 104 104
Size: 5237
PvuII CAG'CTG
Cuts at: 885 2662 3022 885
Size: 1777 360 3100
Fragments arranged by size:
3100 1777 360
RsrII CG'GwC_CG
Cuts at: 3432 3432
Size: 5237
SacI G_AGCT'C
Cuts at: 2251 2251
Size: 5237
SalI G'TCGA_C
Cuts at: 2274 2274
Size: 5237
SapI GCTCTTCn'nnn_
Cuts at: 1881 3260 3470 1881
Size: 1379 210 3648
Fragments arranged by size:
3648 1379 210
SbfI CC_TGCA'GG
Cuts at: 2284 2284
Size: 5237
ScaI AGT'ACT
Cuts at: 114 783 1414 4754 114
Size: 669 631 3340 597
Fragments arranged by size:
3340 669 631 597
SfcI C'TryA_G
Cuts at: 33 116 1212 2280 2965 3958 4149 33
Size: 83 1096 1068 685 993 191 1121
Fragments arranged by size:
1121 1096 1068 993 685 191 83
SfoI GGC'GCC
Cuts at: 2669 2917 2669
Size: 248 4989
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 5237
SmaI CCC'GGG
Cuts at: 2259 2259
Size: 5237
SmlI C'TyrA_G
Cuts at: 309 334 2392 3307 3799 4061 309
Size: 25 2058 915 492 262 1485
Fragments arranged by size:
2058 1485 915 492 262 25
SpeI A'CTAG_T
Cuts at: 67 2206 67
Size: 2139 3098
SphI G_CATG'C
Cuts at: 2290 2790 2290
Size: 500 4737
SspI AAT'ATT
Cuts at: 152 352 152
Size: 200 5037
StyI C'CwwG_G
Cuts at: 427 527 2371 2725 427
Size: 100 1844 354 2939
Fragments arranged by size:
2939 1844 354 100
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 349 420 1459 2820 2953 3412 3621 349
Size: 71 1039 1361 133 459 209 1965
Fragments arranged by size:
1965 1361 1039 459 209 133 71
TatI w'GTAC_w
Cuts at: 112 781 1412 1479 4752 112
Size: 669 631 67 3273 597
Fragments arranged by size:
3273 669 631 597 67
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 899 931 4754 4786 899
Size: 32 3823 32 1350
Fragments arranged by size:
3823 1350 32 32
Tth111I GACn'n_nGTC
Cuts at: 3034 3034
Size: 5237
XbaI T'CTAG_A
Cuts at: 1951 2268 1951
Size: 317 4920
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2732 2732
Size: 5237
XhoI C'TCGA_G
Cuts at: 334 334
Size: 5237
XmaI C'CCGG_G
Cuts at: 2257 2257
Size: 5237
Enzymes that do cut:
AccI Acc65I AclI AcuI AflIII AgeI AlwNI ApaLI
ApoI AseI AsiSI AvaI BaeI BamHI BanI BanII
BbeI BbsI BciVI BclI BfrBI BglII BlpI Bme1580I
BmrI BplI BpmI BpuEI BsaI BsaAI BsaBI BsaHI
BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI Bsp1286I
BspEI BspHI BspMI BsrBI BsrDI BsrFI BssSI BstAPI
BstBI BstYI BtgI BtgZI BtsI ClaI CspCI DraI
DraIII DrdI EaeI EagI EarI EciI EcoICRI Eco57MI
EcoNI EcoO109I EcoRI FalI FspI HaeII Hin4I HincII
HindIII KasI KpnI MluI MmeI MscI MslI MspA1I
NaeI NarI NcoI NgoMIV NsiI NspI PciI PmeI
PpiI PshAI PspXI PstI PvuI PvuII RsrII SacI
SalI SapI SbfI ScaI SfcI SfoI SgfI SmaI
SmlI SpeI SphI SspI StyI TaqII TatI TstI
Tth111I XbaI XcmI XhoI XmaI
Enzymes that do not cut:
AarI AatII AfeI AflII AhdI AleI AlfI AloI
ApaI AscI AvrII BbvCI BcgI BglI BmgBI BmtI
Bpu10I BsiWI BsmI BsmBI BsrGI BssHII BstEII BstXI
BstZ17I Bsu36I EcoRV FseI FspAI HpaI MfeI NdeI
NheI NotI NruI PacI PasI PflMI PfoI PmlI
PpuMI PsiI PspOMI PsrI SacII SanDI SexAI SfiI
SgrAI SnaBI SrfI StuI SwaI XmnI ZraI