Restriction map for
pF1KB9985
(Circular) (Six-base) MAPSORT of: pF1KB9985.seq Check: 410 from: 1 to: 4829
pF1KB9985 4829 bp
With 182 enzymes: SgfI *
April 24, 2009 13:09 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 1411 1411
Size: 4829
AatII G_ACGT'C
Cuts at: 1301 1301
Size: 4829
AccI GT'mk_AC
Cuts at: 743 1867 743
Size: 1124 3705
Acc65I G'GTAC_C
Cuts at: 1845 1845
Size: 4829
AclI AA'CG_TT
Cuts at: 4669 4669
Size: 4829
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1017 2654 3086 3833 4101 1017
Size: 1637 432 747 268 1745
Fragments arranged by size:
1745 1637 747 432 268
AflIII A'CryG_T
Cuts at: 1010 2181 3285 4262 1010
Size: 1171 1104 977 1577
Fragments arranged by size:
1577 1171 1104 977
AgeI A'CCGG_T
Cuts at: 3217 3217
Size: 4829
AleI CACnn'nnGTG
Cuts at: 1015 1015
Size: 4829
AlwNI CAG_nnn'CTG
Cuts at: 1165 2134 3701 1165
Size: 969 1567 2293
Fragments arranged by size:
2293 1567 969
ApaLI G'TGCA_C
Cuts at: 3599 3599
Size: 4829
ApoI r'AATT_y
Cuts at: 621 852 1807 1833 4182 4438 621
Size: 231 955 26 2349 256 1012
Fragments arranged by size:
2349 1012 955 256 231 26
AseI AT'TA_AT
Cuts at: 20 20
Size: 4829
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 4829
AvaI C'yCGr_G
Cuts at: 1767 1849 1767
Size: 82 4747
BamHI G'GATC_C
Cuts at: 1785 1854 1785
Size: 69 4760
BanI G'GyrC_C
Cuts at: 547 1311 1845 2259 2507 2542 547
Size: 764 534 414 248 35 2834
Fragments arranged by size:
2834 764 534 414 248 35
BanII G_rGCy'C
Cuts at: 382 1843 2873 382
Size: 1461 1030 2338
Fragments arranged by size:
2338 1461 1030
BbeI G_GCGC'C
Cuts at: 2263 2511 2263
Size: 248 4581
BbsI GAAGACnn'nnnn_
Cuts at: 242 564 627 756 242
Size: 322 63 129 4315
Fragments arranged by size:
4315 322 129 63
BciVI GTATCCnnnnn_n'
Cuts at: 2727 3488 4829 2727
Size: 761 1341 2727
Fragments arranged by size:
2727 1341 761
BclI T'GATC_A
Cuts at: 2349 2349
Size: 4829
BglII A'GATC_T
Cuts at: 879 2344 4381 879
Size: 1465 2037 1327
Fragments arranged by size:
2037 1465 1327
BlpI GC'TnA_GC
Cuts at: 1941 1941
Size: 4829
Bme1580I G_kGCm'C
Cuts at: 2454 2547 3603 2454
Size: 93 1056 3680
Fragments arranged by size:
3680 1056 93
BmrI ACTGGGnnnn_n'
Cuts at: 280 2041 2122 2457 4601 280
Size: 1761 81 335 2144 508
Fragments arranged by size:
2144 1761 508 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3196 3196
Size: 4829
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1204 1204
Size: 4829
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2005 2884 3376 3674 2005
Size: 879 492 298 3160
Fragments arranged by size:
3160 879 492 298
BsaI GGTCTCn'nnnn_
Cuts at: 1565 4533 1565
Size: 2968 1861
BsaAI yAC'GTr
Cuts at: 798 1013 2182 798
Size: 215 1169 3445
Fragments arranged by size:
3445 1169 215
BsaBI GATnn'nnATC
Cuts at: 6 2343 4380 6
Size: 2337 2037 455
Fragments arranged by size:
2337 2037 455
BsaHI Gr'CG_yC
Cuts at: 1298 2260 2508 3210 4706 1298
Size: 962 248 702 1496 1421
Fragments arranged by size:
1496 1421 962 702 248
BsaWI w'CCGG_w
Cuts at: 2018 2539 3217 3491 3638 4049 2018
Size: 521 678 274 147 411 2798
Fragments arranged by size:
2798 678 521 411 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 323 353 1808 1838 323
Size: 30 1455 30 3314
Fragments arranged by size:
3314 1455 30 30
BseYI C'CCAG_C
Cuts at: 192 1575 2254 3012 3589 4085 192
Size: 1383 679 758 577 496 936
Fragments arranged by size:
1383 936 758 679 577 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 361 361
Size: 4829
BsiEI CG_ry'CG
Cuts at: 104 849 2371 2417 3625 104
Size: 745 1522 46 1208 1308
Fragments arranged by size:
1522 1308 1208 745 46
BsiHKAI G_wGCw'C
Cuts at: 230 1022 1549 1843 2621 2811 3603 230
Size: 792 527 294 778 190 792 1456
Fragments arranged by size:
1456 792 792 778 527 294 190
BsmI GAATG_Cn'
Cuts at: 1364 1364
Size: 4829
BsmBI CGTCTCn'nnnn_
Cuts at: 1464 1464
Size: 4829
Bsp1286I G_dGCh'C
Cuts at: 230 382 1022 1549 1843 2454 2547 2621 2811
Size: 152 640 527 294 611 93 74 190
Cuts at: 2811 2873 3603 230
Size: 62 730 1456
Fragments arranged by size:
1456 730 640 611 527 294 190 152
93 74 62
BspEI T'CCGG_A
Cuts at: 4049 4049
Size: 4829
BspHI T'CATG_A
Cuts at: 183 909 4005 183
Size: 726 3096 1007
Fragments arranged by size:
3096 1007 726
BspMI ACCTGCnnnn'nnnn_
Cuts at: 1173 1360 1411 1879 2093 2395 2776 1173
Size: 187 51 468 214 302 381 3226
Fragments arranged by size:
3226 468 381 302 214 187 51
BsrBI CCG'CTC
Cuts at: 50 3121 3175 4659 4824 50
Size: 3071 54 1484 165 55
Fragments arranged by size:
3071 1484 165 55 54
BsrDI GCAATG_nn'
Cuts at: 204 2741 204
Size: 2537 2292
BsrFI r'CCGG_y
Cuts at: 550 1308 2827 3008 3217 550
Size: 758 1519 181 209 2162
Fragments arranged by size:
2162 1519 758 209 181
BsrGI T'GTAC_A
Cuts at: 1661 1661
Size: 4829
BssSI C'ACGA_G
Cuts at: 373 1542 3100 3458 373
Size: 1169 1558 358 1744
Fragments arranged by size:
1744 1558 1169 358
BstAPI GCAn_nnn'nTGC
Cuts at: 2206 2206
Size: 4829
BstBI TT'CG_AA
Cuts at: 3190 3190
Size: 4829
BstXI CCAn_nnnn'nTGG
Cuts at: 646 646
Size: 4829
BstYI r'GATC_y
Cuts at: 1 879 1785 1854 2321 2344 2679 2925 3937
Size: 878 906 69 467 23 335 246 1012
Cuts at: 3937 4023 4035 4381 1
Size: 86 12 346 449
Fragments arranged by size:
1012 906 878 467 449 346 335 246
86 69 23 12
BtgI C'CryG_G
Cuts at: 107 1315 1370 107
Size: 1208 55 3566
Fragments arranged by size:
3566 1208 55
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 759 2779 2960 3123 759
Size: 2020 181 163 2465
Fragments arranged by size:
2465 2020 181 163
BtsI GCAGTG_nn'
Cuts at: 2072 2072
Size: 4829
DraI TTT'AAA
Cuts at: 1812 1828 1812
Size: 16 4813
DrdI GACnn_nn'nnGTC
Cuts at: 519 2535 3393 519
Size: 2016 858 1955
Fragments arranged by size:
2016 1955 858
EaeI y'GGCC_r
Cuts at: 552 1230 1628 2414 2588 2979 3006 4351 552
Size: 678 398 786 174 391 27 1345 1030
Fragments arranged by size:
1345 1030 786 678 398 391 174 27
EagI C'GGCC_G
Cuts at: 2414 2414
Size: 4829
EarI CTCTTCn'nnn_
Cuts at: 2852 3062 2852
Size: 210 4619
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 1067 3037 3347 3493 4639 1067
Size: 1970 310 146 1146 1257
Fragments arranged by size:
1970 1257 1146 310 146
EcoICRI GAG'CTC
Cuts at: 1841 1841
Size: 4829
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1017 1204 2654 3086 3833 4101 1017
Size: 187 1450 432 747 268 1745
Fragments arranged by size:
1745 1450 747 432 268 187
EcoO109I rG'GnC_Cy
Cuts at: 1069 1162 1968 1069
Size: 93 806 3930
Fragments arranged by size:
3930 806 93
EcoRI G'AATT_C
Cuts at: 1833 1833
Size: 4829
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 975 2848 975
Size: 1873 2956
FspI TGC'GCA
Cuts at: 2610 2610
Size: 4829
HaeII r_GCGC'y
Cuts at: 1607 2263 2511 3533 4512 1607
Size: 656 248 1022 979 1924
Fragments arranged by size:
1924 1022 979 656 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 125 157 897 929 1564 1596 2671 2701 2703
Size: 32 740 32 635 32 1075 30 2
Cuts at: 2703 2733 125
Size: 30 2221
Fragments arranged by size:
2221 1075 740 635 32 32 32 30
30 2
HincII GTy'rAC
Cuts at: 1278 1868 1278
Size: 590 4239
HpaI GTT'AAC
Cuts at: 1278 1278
Size: 4829
KasI G'GCGC_C
Cuts at: 2259 2507 2259
Size: 248 4581
KpnI G_GTAC'C
Cuts at: 1849 1849
Size: 4829
MfeI C'AATT_G
Cuts at: 1253 1253
Size: 4829
MluI A'CGCG_T
Cuts at: 4262 4262
Size: 4829
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 884 3500 3684 884
Size: 2616 184 2029
Fragments arranged by size:
2616 2029 184
MscI TGG'CCA
Cuts at: 1232 1630 2590 1232
Size: 398 960 3471
Fragments arranged by size:
3471 960 398
MslI CAynn'nnrTG
Cuts at: 733 1013 1015 1288 2818 2945 733
Size: 280 2 273 1530 127 2617
Fragments arranged by size:
2617 1530 280 273 127 2
MspA1I CmG'CkG
Cuts at: 1940 2254 2614 3627 3872 1940
Size: 314 360 1013 245 2897
Fragments arranged by size:
2897 1013 360 314 245
NaeI GCC'GGC
Cuts at: 3010 3010
Size: 4829
NarI GG'CG_CC
Cuts at: 2260 2508 2260
Size: 248 4581
NcoI C'CATG_G
Cuts at: 107 1315 107
Size: 1208 3621
NdeI CA'TA_TG
Cuts at: 765 765
Size: 4829
NgoMIV G'CCGG_C
Cuts at: 3008 3008
Size: 4829
NspI r_CATG'y
Cuts at: 1882 2382 3289 1882
Size: 500 907 3422
Fragments arranged by size:
3422 907 500
PasI CC'CwG_GG
Cuts at: 1515 1515
Size: 4829
PciI A'CATG_T
Cuts at: 3285 3285
Size: 4829
PflMI CCAn_nnn'nTGG
Cuts at: 666 666
Size: 4829
PfoI T'CCnGG_A
Cuts at: 322 322
Size: 4829
PmeI GTTT'AAAC
Cuts at: 1828 1828
Size: 4829
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3968 4000 3968
Size: 32 4797
PpuMI rG'GwC_Cy
Cuts at: 1069 1162 1069
Size: 93 4736
PshAI GACnn'nnGTC
Cuts at: 1297 1297
Size: 4829
PstI C_TGCA'G
Cuts at: 469 1062 1432 1876 2561 469
Size: 593 370 444 685 2737
Fragments arranged by size:
2737 685 593 444 370
PvuI CG_AT'CG
Cuts at: 104 849 104
Size: 745 4084
PvuII CAG'CTG
Cuts at: 2254 2614 2254
Size: 360 4469
RsrII CG'GwC_CG
Cuts at: 3024 3024
Size: 4829
SacI G_AGCT'C
Cuts at: 1843 1843
Size: 4829
SalI G'TCGA_C
Cuts at: 1866 1866
Size: 4829
SapI GCTCTTCn'nnn_
Cuts at: 2852 3062 2852
Size: 210 4619
SbfI CC_TGCA'GG
Cuts at: 1876 1876
Size: 4829
ScaI AGT'ACT
Cuts at: 4346 4346
Size: 4829
SfcI C'TryA_G
Cuts at: 33 465 744 1058 1428 1872 2557 3550 3741
Size: 432 279 314 370 444 685 993 191
Cuts at: 3741 33
Size: 1121
Fragments arranged by size:
1121 993 685 444 432 370 314 279
191
SfoI GGC'GCC
Cuts at: 2261 2509 2261
Size: 248 4581
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 4829
SmaI CCC'GGG
Cuts at: 1769 1851 1769
Size: 82 4747
SmlI C'TyrA_G
Cuts at: 1984 2899 3391 3653 1984
Size: 915 492 262 3160
Fragments arranged by size:
3160 915 492 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 4829
SphI G_CATG'C
Cuts at: 1882 2382 1882
Size: 500 4329
StuI AGG'CCT
Cuts at: 1727 1727
Size: 4829
StyI C'CwwG_G
Cuts at: 107 1315 1356 1963 2317 107
Size: 1208 41 607 354 2619
Fragments arranged by size:
2619 1208 607 354 41
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 1110 1429 2412 2545 3004 3213 1110
Size: 319 983 133 459 209 2726
Fragments arranged by size:
2726 983 459 319 209 133
TatI w'GTAC_w
Cuts at: 1661 4344 1661
Size: 2683 2146
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 323 355 1438 1470 1485 1517 4346 4378 323
Size: 32 1083 32 15 32 2829 32 774
Fragments arranged by size:
2829 1083 774 32 32 32 32 15
Tth111I GACn'n_nGTC
Cuts at: 1073 1342 2626 1073
Size: 269 1284 3276
Fragments arranged by size:
3276 1284 269
XbaI T'CTAG_A
Cuts at: 1860 1860
Size: 4829
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2324 2324
Size: 4829
XmaI C'CCGG_G
Cuts at: 1767 1849 1767
Size: 82 4747
ZraI GAC'GTC
Cuts at: 1299 1299
Size: 4829
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AflIII AgeI
AleI AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI
BanI BanII BbeI BbsI BciVI BclI BglII BlpI
Bme1580I BmrI BplI BpmI BpuEI BsaI BsaAI BsaBI
BsaHI BsaWI BsaXI BseYI BsgI BsiEI BsiHKAI BsmI
BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI
BsrGI BssSI BstAPI BstBI BstXI BstYI BtgI BtgZI
BtsI DraI DrdI EaeI EagI EarI EciI EcoICRI
Eco57MI EcoO109I EcoRI FalI FspI HaeII Hin4I HincII
HpaI KasI KpnI MfeI MluI MmeI MscI MslI
MspA1I NaeI NarI NcoI NdeI NgoMIV NspI PasI
PciI PflMI PfoI PmeI PpiI PpuMI PshAI PstI
PvuI PvuII RsrII SacI SalI SapI SbfI ScaI
SfcI SfoI SgfI SmaI SmlI SpeI SphI StuI
StyI TaqII TatI TstI Tth111I XbaI XcmI XmaI
ZraI
Enzymes that do not cut:
AfeI AflII AhdI AlfI AloI ApaI AscI AvrII
BaeI BbvCI BcgI BfrBI BglI BmgBI BmtI Bpu10I
BseRI BsiWI BssHII BstEII BstZ17I Bsu36I ClaI CspCI
DraIII EcoNI EcoRV FseI FspAI HindIII NheI NotI
NruI NsiI PacI PmlI PsiI PspOMI PspXI PsrI
SacII SanDI SexAI SfiI SgrAI SnaBI SrfI SspI
SwaI XhoI XmnI