Restriction map for
pF1KB9986
(Circular) (Six-base) MAPSORT of: pF1KB9986.seq Check: 5154 from: 1 to: 5249
pF1KB9986 5249 bp
With 182 enzymes: SgfI *
October 7, 2009 14:27 ..
AatII G_ACGT'C
Cuts at: 1212 1212
Size: 5249
AccI GT'mk_AC
Cuts at: 1212 1404 2287 1212
Size: 192 883 4174
Fragments arranged by size:
4174 883 192
Acc65I G'GTAC_C
Cuts at: 2265 2265
Size: 5249
AclI AA'CG_TT
Cuts at: 5089 5089
Size: 5249
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 466 1075 1232 1657 1681 1836 3074 3506 4253
Size: 609 157 425 24 155 1238 432 747
Cuts at: 4253 4521 466
Size: 268 1194
Fragments arranged by size:
1238 1194 747 609 432 425 268 157
155 24
AflII C'TTAA_G
Cuts at: 1128 1128
Size: 5249
AflIII A'CryG_T
Cuts at: 1429 2601 3705 4682 1429
Size: 1172 1104 977 1996
Fragments arranged by size:
1996 1172 1104 977
AgeI A'CCGG_T
Cuts at: 3637 3637
Size: 5249
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1901 1901
Size: 5249
AlwNI CAG_nnn'CTG
Cuts at: 1035 1485 2086 2554 4121 1035
Size: 450 601 468 1567 2163
Fragments arranged by size:
2163 1567 601 468 450
ApaLI G'TGCA_C
Cuts at: 4019 4019
Size: 5249
ApoI r'AATT_y
Cuts at: 331 366 404 835 1173 1750 2103 2253 4602
Size: 35 38 431 338 577 353 150 2349
Cuts at: 4602 4858 331
Size: 256 722
Fragments arranged by size:
2349 722 577 431 353 338 256 150
38 35
AseI AT'TA_AT
Cuts at: 20 20
Size: 5249
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 5249
AvaI C'yCGr_G
Cuts at: 2269 2269
Size: 5249
BamHI G'GATC_C
Cuts at: 2274 2274
Size: 5249
BanI G'GyrC_C
Cuts at: 2265 2679 2927 2962 2265
Size: 414 248 35 4552
Fragments arranged by size:
4552 414 248 35
BanII G_rGCy'C
Cuts at: 2263 3293 2263
Size: 1030 4219
BbeI G_GCGC'C
Cuts at: 2683 2931 2683
Size: 248 5001
BbsI GAAGACnn'nnnn_
Cuts at: 694 1354 1684 2212 694
Size: 660 330 528 3731
Fragments arranged by size:
3731 660 528 330
BbvCI CC'TCA_GC
Cuts at: 1486 1486
Size: 5249
BciVI GTATCCnnnnn_n'
Cuts at: 1403 3147 3908 5249 1403
Size: 1744 761 1341 1403
Fragments arranged by size:
1744 1403 1341 761
BclI T'GATC_A
Cuts at: 2769 2769
Size: 5249
BfrBI ATG'CAT
Cuts at: 1468 1468
Size: 5249
BglI GCCn_nnn'nGGC
Cuts at: 1668 1668
Size: 5249
BglII A'GATC_T
Cuts at: 170 2764 4801 170
Size: 2594 2037 618
Fragments arranged by size:
2594 2037 618
BlpI GC'TnA_GC
Cuts at: 706 2361 706
Size: 1655 3594
Bme1580I G_kGCm'C
Cuts at: 2874 2967 4023 2874
Size: 93 1056 4100
Fragments arranged by size:
4100 1056 93
BmgBI CAC'GTC
Cuts at: 621 621
Size: 5249
BmrI ACTGGGnnnn_n'
Cuts at: 1599 2461 2542 2877 5021 1599
Size: 862 81 335 2144 1827
Fragments arranged by size:
2144 1827 862 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3616 3616
Size: 5249
Bpu10I CC'TnA_GC
Cuts at: 1486 1486
Size: 5249
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1543 1924 2425 3304 3796 4094 1543
Size: 381 501 879 492 298 2698
Fragments arranged by size:
2698 879 501 492 381 298
BsaI GGTCTCn'nnnn_
Cuts at: 4953 4953
Size: 5249
BsaAI yAC'GTr
Cuts at: 1233 2602 1233
Size: 1369 3880
BsaBI GATnn'nnATC
Cuts at: 6 2763 4800 6
Size: 2757 2037 455
Fragments arranged by size:
2757 2037 455
BsaHI Gr'CG_yC
Cuts at: 1209 2680 2928 3630 5126 1209
Size: 1471 248 702 1496 1332
Fragments arranged by size:
1496 1471 1332 702 248
BsaWI w'CCGG_w
Cuts at: 2438 2959 3637 3911 4058 4469 2438
Size: 521 678 274 147 411 3218
Fragments arranged by size:
3218 678 521 411 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1817 1847 2228 2258 1817
Size: 30 381 30 4808
Fragments arranged by size:
4808 381 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 619 734 1474 1606 619
Size: 115 740 132 4262
Fragments arranged by size:
4262 740 132 115
BseYI C'CCAG_C
Cuts at: 1121 2674 3432 4009 4505 1121
Size: 1553 758 577 496 1865
Fragments arranged by size:
1865 1553 758 577 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1898 1999 1898
Size: 101 5148
BsiEI CG_ry'CG
Cuts at: 104 2791 2837 4045 104
Size: 2687 46 1208 1308
Fragments arranged by size:
2687 1308 1208 46
BsiHKAI G_wGCw'C
Cuts at: 959 2263 3041 3231 4023 959
Size: 1304 778 190 792 2185
Fragments arranged by size:
2185 1304 792 778 190
BsmI GAATG_Cn'
Cuts at: 1973 1973
Size: 5249
Bsp1286I G_dGCh'C
Cuts at: 959 2263 2874 2967 3041 3231 3293 4023 959
Size: 1304 611 93 74 190 62 730 2185
Fragments arranged by size:
2185 1304 730 611 190 93 74 62
BspEI T'CCGG_A
Cuts at: 4469 4469
Size: 5249
BspHI T'CATG_A
Cuts at: 447 1056 4425 447
Size: 609 3369 1271
Fragments arranged by size:
3369 1271 609
BspMI ACCTGCnnnn'nnnn_
Cuts at: 1328 2299 2513 2815 3196 1328
Size: 971 214 302 381 3381
Fragments arranged by size:
3381 971 381 302 214
BsrBI CCG'CTC
Cuts at: 50 3541 3595 5079 5244 50
Size: 3491 54 1484 165 55
Fragments arranged by size:
3491 1484 165 55 54
BsrDI GCAATG_nn'
Cuts at: 511 3161 511
Size: 2650 2599
BsrFI r'CCGG_y
Cuts at: 3247 3428 3637 3247
Size: 181 209 4859
Fragments arranged by size:
4859 209 181
BsrGI T'GTAC_A
Cuts at: 1426 1426
Size: 5249
BssSI C'ACGA_G
Cuts at: 2044 3520 3878 2044
Size: 1476 358 3415
Fragments arranged by size:
3415 1476 358
BstAPI GCAn_nnn'nTGC
Cuts at: 2107 2626 2107
Size: 519 4730
BstBI TT'CG_AA
Cuts at: 239 3610 239
Size: 3371 1878
BstYI r'GATC_y
Cuts at: 1 170 2274 2741 2764 3099 3345 4357 4443
Size: 169 2104 467 23 335 246 1012 86
Cuts at: 4443 4455 4801 1
Size: 12 346 449
Fragments arranged by size:
2104 1012 467 449 346 335 246 169
86 23 12
Bsu36I CC'TnA_GG
Cuts at: 218 218
Size: 5249
BtgI C'CryG_G
Cuts at: 107 107
Size: 5249
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 3199 3380 3543 3199
Size: 181 163 4905
Fragments arranged by size:
4905 181 163
BtsI GCAGTG_nn'
Cuts at: 2492 2492
Size: 5249
DraI TTT'AAA
Cuts at: 1008 1883 2248 1008
Size: 875 365 4009
Fragments arranged by size:
4009 875 365
DrdI GACnn_nn'nnGTC
Cuts at: 2955 3813 2955
Size: 858 4391
EaeI y'GGCC_r
Cuts at: 2834 3008 3399 3426 4771 2834
Size: 174 391 27 1345 3312
Fragments arranged by size:
3312 1345 391 174 27
EagI C'GGCC_G
Cuts at: 2834 2834
Size: 5249
EarI CTCTTCn'nnn_
Cuts at: 1383 1388 3272 3482 1383
Size: 5 1884 210 3150
Fragments arranged by size:
3150 1884 210 5
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 3457 3767 3913 5059 3457
Size: 310 146 1146 3647
Fragments arranged by size:
3647 1146 310 146
EcoICRI GAG'CTC
Cuts at: 2261 2261
Size: 5249
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 466 1075 1232 1657 1681 1836 3074 3506 4253
Size: 609 157 425 24 155 1238 432 747
Cuts at: 4253 4521 466
Size: 268 1194
Fragments arranged by size:
1238 1194 747 609 432 425 268 157
155 24
EcoO109I rG'GnC_Cy
Cuts at: 477 2388 477
Size: 1911 3338
EcoRI G'AATT_C
Cuts at: 366 2253 366
Size: 1887 3362
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 153 740 3268 153
Size: 587 2528 2134
Fragments arranged by size:
2528 2134 587
FspI TGC'GCA
Cuts at: 3030 3030
Size: 5249
HaeII r_GCGC'y
Cuts at: 2683 2931 3953 4932 2683
Size: 248 1022 979 3000
Fragments arranged by size:
3000 1022 979 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 580 612 1453 1485 1815 1847 3091 3121 3123
Size: 32 841 32 330 32 1244 30 2
Cuts at: 3123 3153 580
Size: 30 2676
Fragments arranged by size:
2676 1244 841 330 32 32 32 30
30 2
HincII GTy'rAC
Cuts at: 823 1405 2288 823
Size: 582 883 3784
Fragments arranged by size:
3784 883 582
HindIII A'AGCT_T
Cuts at: 229 777 1244 229
Size: 548 467 4234
Fragments arranged by size:
4234 548 467
KasI G'GCGC_C
Cuts at: 2679 2927 2679
Size: 248 5001
KpnI G_GTAC'C
Cuts at: 2269 2269
Size: 5249
MluI A'CGCG_T
Cuts at: 4682 4682
Size: 5249
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 433 1230 3920 4104 433
Size: 797 2690 184 1578
Fragments arranged by size:
2690 1578 797 184
MscI TGG'CCA
Cuts at: 3010 3010
Size: 5249
MslI CAynn'nnrTG
Cuts at: 830 1883 3238 3365 830
Size: 1053 1355 127 2714
Fragments arranged by size:
2714 1355 1053 127
MspA1I CmG'CkG
Cuts at: 1482 2083 2360 2674 3034 4047 4292 1482
Size: 601 277 314 360 1013 245 2439
Fragments arranged by size:
2439 1013 601 360 314 277 245
NaeI GCC'GGC
Cuts at: 3430 3430
Size: 5249
NarI GG'CG_CC
Cuts at: 2680 2928 2680
Size: 248 5001
NcoI C'CATG_G
Cuts at: 107 107
Size: 5249
NdeI CA'TA_TG
Cuts at: 501 1334 1447 501
Size: 833 113 4303
Fragments arranged by size:
4303 833 113
NgoMIV G'CCGG_C
Cuts at: 3428 3428
Size: 5249
NsiI A_TGCA'T
Cuts at: 1470 1470
Size: 5249
NspI r_CATG'y
Cuts at: 1327 1433 2302 2802 3709 1327
Size: 106 869 500 907 2867
Fragments arranged by size:
2867 907 869 500 106
PciI A'CATG_T
Cuts at: 1429 3705 1429
Size: 2276 2973
PflMI CCAn_nnn'nTGG
Cuts at: 1842 1842
Size: 5249
PmeI GTTT'AAAC
Cuts at: 2248 2248
Size: 5249
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4388 4420 4388
Size: 32 5217
PsiI TTA'TAA
Cuts at: 1081 2188 1081
Size: 1107 4142
PsrI GAACnnnnnnTACnnnnnnn_nnnnn'
Cuts at: 1016 1048 1016
Size: 32 5217
PstI C_TGCA'G
Cuts at: 2296 2981 2296
Size: 685 4564
PvuI CG_AT'CG
Cuts at: 104 104
Size: 5249
PvuII CAG'CTG
Cuts at: 1482 2083 2674 3034 1482
Size: 601 591 360 3697
Fragments arranged by size:
3697 601 591 360
RsrII CG'GwC_CG
Cuts at: 3444 3444
Size: 5249
SacI G_AGCT'C
Cuts at: 2263 2263
Size: 5249
SalI G'TCGA_C
Cuts at: 1403 2286 1403
Size: 883 4366
SapI GCTCTTCn'nnn_
Cuts at: 3272 3482 3272
Size: 210 5039
SbfI CC_TGCA'GG
Cuts at: 2296 2296
Size: 5249
ScaI AGT'ACT
Cuts at: 794 1760 4766 794
Size: 966 3006 1277
Fragments arranged by size:
3006 1277 966
SfcI C'TryA_G
Cuts at: 33 2292 2977 3970 4161 33
Size: 2259 685 993 191 1121
Fragments arranged by size:
2259 1121 993 685 191
SfoI GGC'GCC
Cuts at: 2681 2929 2681
Size: 248 5001
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 5249
SmaI CCC'GGG
Cuts at: 2271 2271
Size: 5249
SmlI C'TyrA_G
Cuts at: 1128 1522 1939 2404 3319 3811 4073 1128
Size: 394 417 465 915 492 262 2304
Fragments arranged by size:
2304 915 492 465 417 394 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 5249
SphI G_CATG'C
Cuts at: 2302 2802 2302
Size: 500 4749
StyI C'CwwG_G
Cuts at: 107 1566 2383 2737 107
Size: 1459 817 354 2619
Fragments arranged by size:
2619 1459 817 354
SwaI ATTT'AAAT
Cuts at: 1883 1883
Size: 5249
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 640 2832 2965 3424 3633 640
Size: 2192 133 459 209 2256
Fragments arranged by size:
2256 2192 459 209 133
TatI w'GTAC_w
Cuts at: 792 1421 1426 1758 1847 4764 792
Size: 629 5 332 89 2917 1277
Fragments arranged by size:
2917 1277 629 332 89 5
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 1817 1849 4766 4798 1817
Size: 32 2917 32 2268
Fragments arranged by size:
2917 2268 32 32
Tth111I GACn'n_nGTC
Cuts at: 3046 3046
Size: 5249
XbaI T'CTAG_A
Cuts at: 2280 2280
Size: 5249
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2744 2744
Size: 5249
XmaI C'CCGG_G
Cuts at: 2269 2269
Size: 5249
XmnI GAAnn'nnTTC
Cuts at: 614 1342 1531 1808 614
Size: 728 189 277 4055
Fragments arranged by size:
4055 728 277 189
ZraI GAC'GTC
Cuts at: 1210 1210
Size: 5249
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AlfI AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI
BanI BanII BbeI BbsI BbvCI BciVI BclI BfrBI
BglI BglII BlpI Bme1580I BmgBI BmrI BplI Bpu10I
BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI
BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI
BspMI BsrBI BsrDI BsrFI BsrGI BssSI BstAPI BstBI
BstYI Bsu36I BtgI BtgZI BtsI DraI DrdI EaeI
EagI EarI EciI EcoICRI Eco57MI EcoO109I EcoRI FalI
FspI HaeII Hin4I HincII HindIII KasI KpnI MluI
MmeI MscI MslI MspA1I NaeI NarI NcoI NdeI
NgoMIV NsiI NspI PciI PflMI PmeI PpiI PsiI
PsrI PstI PvuI PvuII RsrII SacI SalI SapI
SbfI ScaI SfcI SfoI SgfI SmaI SmlI SpeI
SphI StyI SwaI TaqII TatI TstI Tth111I XbaI
XcmI XmaI XmnI ZraI
Enzymes that do not cut:
AarI AfeI AhdI AleI AloI ApaI AscI AvrII
BaeI BcgI BmtI BpmI BsiWI BsmBI BssHII BstEII
BstXI BstZ17I ClaI CspCI DraIII EcoNI EcoRV FseI
FspAI HpaI MfeI NheI NotI NruI PacI PasI
PfoI PmlI PpuMI PshAI PspOMI PspXI SacII SanDI
SexAI SfiI SgrAI SnaBI SrfI SspI StuI XhoI