Restriction map for
pF1KB9989
(Circular) (Six-base) MAPSORT of: pF1KB9989.seq Check: 5416 from: 1 to: 4850
pF1KB9989 4850 bp
With 182 enzymes: SgfI *
October 7, 2009 14:27 ..
AccI GT'mk_AC
Cuts at: 1888 1888
Size: 4850
Acc65I G'GTAC_C
Cuts at: 1866 1866
Size: 4850
AclI AA'CG_TT
Cuts at: 4690 4690
Size: 4850
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 716 2675 3107 3854 4122 716
Size: 1959 432 747 268 1444
Fragments arranged by size:
1959 1444 747 432 268
AflII C'TTAA_G
Cuts at: 1174 1174
Size: 4850
AflIII A'CryG_T
Cuts at: 831 1810 2202 3306 4283 831
Size: 979 392 1104 977 1398
Fragments arranged by size:
1398 1104 979 977 392
AgeI A'CCGG_T
Cuts at: 3238 3238
Size: 4850
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 437 437
Size: 4850
AlwNI CAG_nnn'CTG
Cuts at: 2155 3722 2155
Size: 1567 3283
ApaLI G'TGCA_C
Cuts at: 3620 3620
Size: 4850
ApoI r'AATT_y
Cuts at: 947 1246 1607 1854 4203 4459 947
Size: 299 361 247 2349 256 1338
Fragments arranged by size:
2349 1338 361 299 256 247
AseI AT'TA_AT
Cuts at: 20 20
Size: 4850
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 4850
AvaI C'yCGr_G
Cuts at: 1870 1870
Size: 4850
BamHI G'GATC_C
Cuts at: 1875 1875
Size: 4850
BanI G'GyrC_C
Cuts at: 1866 2280 2528 2563 1866
Size: 414 248 35 4153
Fragments arranged by size:
4153 414 248 35
BanII G_rGCy'C
Cuts at: 1864 2894 1864
Size: 1030 3820
BbeI G_GCGC'C
Cuts at: 2284 2532 2284
Size: 248 4602
BbsI GAAGACnn'nnnn_
Cuts at: 826 1726 826
Size: 900 3950
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 254 288 254
Size: 34 4816
BciVI GTATCCnnnnn_n'
Cuts at: 2748 3509 4850 2748
Size: 761 1341 2748
Fragments arranged by size:
2748 1341 761
BclI T'GATC_A
Cuts at: 2370 2370
Size: 4850
BfrBI ATG'CAT
Cuts at: 520 664 520
Size: 144 4706
BglI GCCn_nnn'nGGC
Cuts at: 112 112
Size: 4850
BglII A'GATC_T
Cuts at: 2365 4402 2365
Size: 2037 2813
BlpI GC'TnA_GC
Cuts at: 1962 1962
Size: 4850
Bme1580I G_kGCm'C
Cuts at: 2475 2568 3624 2475
Size: 93 1056 3701
Fragments arranged by size:
3701 1056 93
BmrI ACTGGGnnnn_n'
Cuts at: 1478 2062 2143 2478 4622 1478
Size: 584 81 335 2144 1706
Fragments arranged by size:
2144 1706 584 335 81
BmtI G_CTAG'C
Cuts at: 1537 1537
Size: 4850
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3217 3217
Size: 4850
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 332 332
Size: 4850
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2026 2905 3397 3695 2026
Size: 879 492 298 3181
Fragments arranged by size:
3181 879 492 298
BsaI GGTCTCn'nnnn_
Cuts at: 358 1831 4554 358
Size: 1473 2723 654
Fragments arranged by size:
2723 1473 654
BsaAI yAC'GTr
Cuts at: 2203 2203
Size: 4850
BsaBI GATnn'nnATC
Cuts at: 6 542 2364 4401 6
Size: 536 1822 2037 455
Fragments arranged by size:
2037 1822 536 455
BsaHI Gr'CG_yC
Cuts at: 977 2281 2529 3231 4727 977
Size: 1304 248 702 1496 1100
Fragments arranged by size:
1496 1304 1100 702 248
BsaWI w'CCGG_w
Cuts at: 279 2039 2560 3238 3512 3659 4070 279
Size: 1760 521 678 274 147 411 1059
Fragments arranged by size:
1760 1059 678 521 411 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1322 1352 1500 1530 1322
Size: 30 148 30 4642
Fragments arranged by size:
4642 148 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 829 829
Size: 4850
BseYI C'CCAG_C
Cuts at: 1285 2275 3033 3610 4106 1285
Size: 990 758 577 496 2029
Fragments arranged by size:
2029 990 758 577 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1004 1175 1360 1793 1004
Size: 171 185 433 4061
Fragments arranged by size:
4061 433 185 171
BsiEI CG_ry'CG
Cuts at: 104 2392 2438 3646 104
Size: 2288 46 1208 1308
Fragments arranged by size:
2288 1308 1208 46
BsiHKAI G_wGCw'C
Cuts at: 1414 1578 1864 2642 2832 3624 1414
Size: 164 286 778 190 792 2640
Fragments arranged by size:
2640 792 778 286 190 164
BsmBI CGTCTCn'nnnn_
Cuts at: 257 257
Size: 4850
Bsp1286I G_dGCh'C
Cuts at: 1414 1578 1864 2475 2568 2642 2832 2894 3624
Size: 164 286 611 93 74 190 62 730
Cuts at: 3624 1414
Size: 2640
Fragments arranged by size:
2640 730 611 286 190 164 93 74
62
BspEI T'CCGG_A
Cuts at: 279 4070 279
Size: 3791 1059
BspHI T'CATG_A
Cuts at: 1752 1788 4026 1752
Size: 36 2238 2576
Fragments arranged by size:
2576 2238 36
BspMI ACCTGCnnnn'nnnn_
Cuts at: 851 1900 2114 2416 2797 851
Size: 1049 214 302 381 2904
Fragments arranged by size:
2904 1049 381 302 214
BsrBI CCG'CTC
Cuts at: 50 3142 3196 4680 4845 50
Size: 3092 54 1484 165 55
Fragments arranged by size:
3092 1484 165 55 54
BsrDI GCAATG_nn'
Cuts at: 1318 2762 1318
Size: 1444 3406
BsrFI r'CCGG_y
Cuts at: 2848 3029 3238 2848
Size: 181 209 4460
Fragments arranged by size:
4460 209 181
BssSI C'ACGA_G
Cuts at: 3121 3479 3121
Size: 358 4492
BstAPI GCAn_nnn'nTGC
Cuts at: 1497 1636 2227 1497
Size: 139 591 4120
Fragments arranged by size:
4120 591 139
BstBI TT'CG_AA
Cuts at: 1323 3211 1323
Size: 1888 2962
BstYI r'GATC_y
Cuts at: 1 1875 2342 2365 2700 2946 3958 4044 4056
Size: 1874 467 23 335 246 1012 86 12
Cuts at: 4056 4402 1
Size: 346 449
Fragments arranged by size:
1874 1012 467 449 346 335 246 86
23 12
Bsu36I CC'TnA_GG
Cuts at: 881 881
Size: 4850
BtgI C'CryG_G
Cuts at: 691 1031 691
Size: 340 4510
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 2800 2981 3144 2800
Size: 181 163 4506
Fragments arranged by size:
4506 181 163
BtsI GCAGTG_nn'
Cuts at: 266 272 1511 1781 2093 266
Size: 6 1239 270 312 3023
Fragments arranged by size:
3023 1239 312 270 6
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 686 721 686
Size: 35 4815
DraI TTT'AAA
Cuts at: 651 1746 1849 651
Size: 1095 103 3652
Fragments arranged by size:
3652 1095 103
DrdI GACnn_nn'nnGTC
Cuts at: 331 2556 3414 331
Size: 2225 858 1767
Fragments arranged by size:
2225 1767 858
EaeI y'GGCC_r
Cuts at: 131 1008 2435 2609 3000 3027 4372 131
Size: 877 1427 174 391 27 1345 609
Fragments arranged by size:
1427 1345 877 609 391 174 27
EagI C'GGCC_G
Cuts at: 2435 2435
Size: 4850
EarI CTCTTCn'nnn_
Cuts at: 734 794 2873 3083 734
Size: 60 2079 210 2501
Fragments arranged by size:
2501 2079 210 60
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 3058 3368 3514 4660 3058
Size: 310 146 1146 3248
Fragments arranged by size:
3248 1146 310 146
EcoICRI GAG'CTC
Cuts at: 1862 1862
Size: 4850
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 332 716 2675 3107 3854 4122 332
Size: 384 1959 432 747 268 1060
Fragments arranged by size:
1959 1060 747 432 384 268
EcoNI CCTnn'n_nnAGG
Cuts at: 1164 1164
Size: 4850
EcoO109I rG'GnC_Cy
Cuts at: 863 1989 863
Size: 1126 3724
EcoRI G'AATT_C
Cuts at: 1854 1854
Size: 4850
EcoRV GAT'ATC
Cuts at: 1119 1452 1119
Size: 333 4517
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 515 843 2869 515
Size: 328 2026 2496
Fragments arranged by size:
2496 2026 328
FspI TGC'GCA
Cuts at: 2631 2631
Size: 4850
HaeII r_GCGC'y
Cuts at: 1077 2284 2532 3554 4533 1077
Size: 1207 248 1022 979 1394
Fragments arranged by size:
1394 1207 1022 979 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 111 143 1500 1532 2692 2722 2724 2754 111
Size: 32 1357 32 1160 30 2 30 2207
Fragments arranged by size:
2207 1357 1160 32 32 30 30 2
HincII GTy'rAC
Cuts at: 908 1371 1889 908
Size: 463 518 3869
Fragments arranged by size:
3869 518 463
KasI G'GCGC_C
Cuts at: 2280 2528 2280
Size: 248 4602
KpnI G_GTAC'C
Cuts at: 1870 1870
Size: 4850
MluI A'CGCG_T
Cuts at: 4283 4283
Size: 4850
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 3521 3705 3521
Size: 184 4666
MscI TGG'CCA
Cuts at: 1010 2611 1010
Size: 1601 3249
MslI CAynn'nnrTG
Cuts at: 467 1787 2839 2966 467
Size: 1320 1052 127 2351
Fragments arranged by size:
2351 1320 1052 127
MspA1I CmG'CkG
Cuts at: 198 1961 2275 2635 3648 3893 198
Size: 1763 314 360 1013 245 1155
Fragments arranged by size:
1763 1155 1013 360 314 245
NaeI GCC'GGC
Cuts at: 3031 3031
Size: 4850
NarI GG'CG_CC
Cuts at: 2281 2529 2281
Size: 248 4602
NcoI C'CATG_G
Cuts at: 691 1031 691
Size: 340 4510
NdeI CA'TA_TG
Cuts at: 510 1622 510
Size: 1112 3738
NgoMIV G'CCGG_C
Cuts at: 3029 3029
Size: 4850
NheI G'CTAG_C
Cuts at: 1533 1533
Size: 4850
NsiI A_TGCA'T
Cuts at: 522 666 522
Size: 144 4706
NspI r_CATG'y
Cuts at: 835 1541 1814 1903 2403 3310 835
Size: 706 273 89 500 907 2375
Fragments arranged by size:
2375 907 706 500 273 89
PciI A'CATG_T
Cuts at: 831 1810 3306 831
Size: 979 1496 2375
Fragments arranged by size:
2375 1496 979
PflMI CCAn_nnn'nTGG
Cuts at: 1548 1548
Size: 4850
PmeI GTTT'AAAC
Cuts at: 1849 1849
Size: 4850
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3989 4021 3989
Size: 32 4818
PpuMI rG'GwC_Cy
Cuts at: 863 863
Size: 4850
PstI C_TGCA'G
Cuts at: 1137 1506 1897 2582 1137
Size: 369 391 685 3405
Fragments arranged by size:
3405 685 391 369
PvuI CG_AT'CG
Cuts at: 104 104
Size: 4850
PvuII CAG'CTG
Cuts at: 2275 2635 2275
Size: 360 4490
RsrII CG'GwC_CG
Cuts at: 3045 3045
Size: 4850
SacI G_AGCT'C
Cuts at: 1864 1864
Size: 4850
SalI G'TCGA_C
Cuts at: 1887 1887
Size: 4850
SapI GCTCTTCn'nnn_
Cuts at: 734 2873 3083 734
Size: 2139 210 2501
Fragments arranged by size:
2501 2139 210
SbfI CC_TGCA'GG
Cuts at: 1897 1897
Size: 4850
ScaI AGT'ACT
Cuts at: 4367 4367
Size: 4850
SfcI C'TryA_G
Cuts at: 33 572 1133 1502 1734 1893 2578 3571 3762
Size: 539 561 369 232 159 685 993 191
Cuts at: 3762 33
Size: 1121
Fragments arranged by size:
1121 993 685 561 539 369 232 191
159
SfoI GGC'GCC
Cuts at: 2282 2530 2282
Size: 248 4602
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 4850
SmaI CCC'GGG
Cuts at: 1872 1872
Size: 4850
SmlI C'TyrA_G
Cuts at: 1174 2005 2920 3412 3674 1174
Size: 831 915 492 262 2350
Fragments arranged by size:
2350 915 831 492 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 4850
SphI G_CATG'C
Cuts at: 1541 1903 2403 1541
Size: 362 500 3988
Fragments arranged by size:
3988 500 362
SspI AAT'ATT
Cuts at: 672 672
Size: 4850
StyI C'CwwG_G
Cuts at: 691 1031 1984 2338 691
Size: 340 953 354 3203
Fragments arranged by size:
3203 953 354 340
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 2433 2566 3025 3234 2433
Size: 133 459 209 4049
Fragments arranged by size:
4049 459 209 133
TatI w'GTAC_w
Cuts at: 1469 4365 1469
Size: 2896 1954
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 1322 1354 4367 4399 1322
Size: 32 3013 32 1773
Fragments arranged by size:
3013 1773 32 32
Tth111I GACn'n_nGTC
Cuts at: 1512 2647 1512
Size: 1135 3715
XbaI T'CTAG_A
Cuts at: 1881 1881
Size: 4850
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 1383 1526 2345 1383
Size: 143 819 3888
Fragments arranged by size:
3888 819 143
XmaI C'CCGG_G
Cuts at: 1870 1870
Size: 4850
Enzymes that do cut:
AccI Acc65I AclI AcuI AflII AflIII AgeI AlfI
AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI BanI
BanII BbeI BbsI BcgI BciVI BclI BfrBI BglI
BglII BlpI Bme1580I BmrI BmtI BplI BpmI BpuEI
BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI
BsgI BsiEI BsiHKAI BsmBI Bsp1286I BspEI BspHI BspMI
BsrBI BsrDI BsrFI BssSI BstAPI BstBI BstYI Bsu36I
BtgI BtgZI BtsI CspCI DraI DrdI EaeI EagI
EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI EcoRV
FalI FspI HaeII Hin4I HincII KasI KpnI MluI
MmeI MscI MslI MspA1I NaeI NarI NcoI NdeI
NgoMIV NheI NsiI NspI PciI PflMI PmeI PpiI
PpuMI PstI PvuI PvuII RsrII SacI SalI SapI
SbfI ScaI SfcI SfoI SgfI SmaI SmlI SpeI
SphI SspI StyI TaqII TatI TstI Tth111I XbaI
XcmI XmaI
Enzymes that do not cut:
AarI AatII AfeI AhdI AleI AloI ApaI AscI
AvrII BaeI BbvCI BmgBI Bpu10I BsiWI BsmI BsrGI
BssHII BstEII BstXI BstZ17I ClaI DraIII FseI FspAI
HindIII HpaI MfeI NotI NruI PacI PasI PfoI
PmlI PshAI PsiI PspOMI PspXI PsrI SacII SanDI
SexAI SfiI SgrAI SnaBI SrfI StuI SwaI XhoI
XmnI ZraI