Restriction map for
pF1KE0002
(Circular) (Six-base) MAPSORT of: pF1KE0002.seq Check: 9013 from: 1 to: 4850
pF1KE0002 4850 bp
With 182 enzymes: SgfI *
March 2, 2010 14:30 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 207 316 207
Size: 109 4741
AccI GT'mk_AC
Cuts at: 1518 1888 1518
Size: 370 4480
Acc65I G'GTAC_C
Cuts at: 1866 1866
Size: 4850
AclI AA'CG_TT
Cuts at: 4690 4690
Size: 4850
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 869 2675 3107 3854 4122 869
Size: 1806 432 747 268 1597
Fragments arranged by size:
1806 1597 747 432 268
AfeI AGC'GCT
Cuts at: 830 830
Size: 4850
AflII C'TTAA_G
Cuts at: 832 832
Size: 4850
AflIII A'CryG_T
Cuts at: 2202 3306 4283 2202
Size: 1104 977 2769
Fragments arranged by size:
2769 1104 977
AgeI A'CCGG_T
Cuts at: 3238 3238
Size: 4850
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1282 1282
Size: 4850
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 613 645 613
Size: 32 4818
AlwNI CAG_nnn'CTG
Cuts at: 222 336 618 798 867 2155 3722 222
Size: 114 282 180 69 1288 1567 1350
Fragments arranged by size:
1567 1350 1288 282 180 114 69
ApaI G_GGCC'C
Cuts at: 231 1036 1326 1495 231
Size: 805 290 169 3586
Fragments arranged by size:
3586 805 290 169
ApaLI G'TGCA_C
Cuts at: 3620 3620
Size: 4850
ApoI r'AATT_y
Cuts at: 424 1854 4203 4459 424
Size: 1430 2349 256 815
Fragments arranged by size:
2349 1430 815 256
AseI AT'TA_AT
Cuts at: 20 20
Size: 4850
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 4850
AvaI C'yCGr_G
Cuts at: 172 230 779 1193 1468 1488 1841 1870 172
Size: 58 549 414 275 20 353 29 3152
Fragments arranged by size:
3152 549 414 353 275 58 29 20
BamHI G'GATC_C
Cuts at: 1875 1875
Size: 4850
BanI G'GyrC_C
Cuts at: 837 1161 1258 1386 1866 2280 2528 2563 837
Size: 324 97 128 480 414 248 35 3124
Fragments arranged by size:
3124 480 414 324 248 128 97 35
BanII G_rGCy'C
Cuts at: 231 1036 1185 1289 1326 1444 1475 1495 1864
Size: 805 149 104 37 118 31 20 369
Cuts at: 1864 2894 231
Size: 1030 2187
Fragments arranged by size:
2187 1030 805 369 149 118 104 37
31 20
BbeI G_GCGC'C
Cuts at: 1390 2284 2532 1390
Size: 894 248 3708
Fragments arranged by size:
3708 894 248
BciVI GTATCCnnnnn_n'
Cuts at: 2748 3509 4850 2748
Size: 761 1341 2748
Fragments arranged by size:
2748 1341 761
BclI T'GATC_A
Cuts at: 338 2370 338
Size: 2032 2818
BglI GCCn_nnn'nGGC
Cuts at: 1680 1680
Size: 4850
BglII A'GATC_T
Cuts at: 1076 2365 4402 1076
Size: 1289 2037 1524
Fragments arranged by size:
2037 1524 1289
BlpI GC'TnA_GC
Cuts at: 1962 1962
Size: 4850
Bme1580I G_kGCm'C
Cuts at: 231 612 1036 1326 1495 1740 2475 2568 3624
Size: 381 424 290 169 245 735 93 1056
Cuts at: 3624 231
Size: 1457
Fragments arranged by size:
1457 1056 735 424 381 290 245 169
93
BmrI ACTGGGnnnn_n'
Cuts at: 1573 2062 2143 2478 4622 1573
Size: 489 81 335 2144 1801
Fragments arranged by size:
2144 1801 489 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 468 3217 468
Size: 2749 2101
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 463 640 820 994 1005 1012 1399 1634 463
Size: 177 180 174 11 7 387 235 3679
Fragments arranged by size:
3679 387 235 180 177 174 11 7
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1459 2026 2905 3397 3695 1459
Size: 567 879 492 298 2614
Fragments arranged by size:
2614 879 567 492 298
BsaI GGTCTCn'nnnn_
Cuts at: 202 1009 4554 202
Size: 807 3545 498
Fragments arranged by size:
3545 807 498
BsaAI yAC'GTr
Cuts at: 2203 2203
Size: 4850
BsaBI GATnn'nnATC
Cuts at: 6 2364 4401 6
Size: 2358 2037 455
Fragments arranged by size:
2358 2037 455
BsaHI Gr'CG_yC
Cuts at: 1370 1387 1484 1515 2281 2529 3231 4727 1370
Size: 17 97 31 766 248 702 1496 1493
Fragments arranged by size:
1496 1493 766 702 248 97 31 17
BsaWI w'CCGG_w
Cuts at: 1399 2039 2560 3238 3512 3659 4070 1399
Size: 640 521 678 274 147 411 2179
Fragments arranged by size:
2179 678 640 521 411 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 370 400 613 643 1511 1541 1749 1779 370
Size: 30 213 30 868 30 208 30 3441
Fragments arranged by size:
3441 868 213 208 30 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1246 1249 1667 1739 1246
Size: 3 418 72 4357
Fragments arranged by size:
4357 418 72 3
BseYI C'CCAG_C
Cuts at: 222 461 476 512 1648 1665 2275 3033 3610
Size: 239 15 36 1136 17 610 758 577
Cuts at: 3610 4106 222
Size: 496 966
Fragments arranged by size:
1136 966 758 610 577 496 239 36
17 15
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 224 625 811 1252 224
Size: 401 186 441 3822
Fragments arranged by size:
3822 441 401 186
BsiEI CG_ry'CG
Cuts at: 104 501 1058 1253 1451 1559 2392 2438 3646
Size: 397 557 195 198 108 833 46 1208
Cuts at: 3646 104
Size: 1308
Fragments arranged by size:
1308 1208 833 557 397 198 195 108
46
BsiHKAI G_wGCw'C
Cuts at: 281 1289 1475 1864 2642 2832 3624 281
Size: 1008 186 389 778 190 792 1507
Fragments arranged by size:
1507 1008 792 778 389 190 186
BsmBI CGTCTCn'nnnn_
Cuts at: 880 880
Size: 4850
Bsp1286I G_dGCh'C
Cuts at: 231 281 612 1036 1185 1289 1326 1444 1475
Size: 50 331 424 149 104 37 118 31
Cuts at: 1475 1495 1740 1864 2475 2568 2642 2832 2894
Size: 20 245 124 611 93 74 190 62
Cuts at: 2894 3624 231
Size: 730 1457
Fragments arranged by size:
1457 730 611 424 331 245 190 149
124 118 104 93 74 62 50 37
31 20
BspEI T'CCGG_A
Cuts at: 4070 4070
Size: 4850
BspHI T'CATG_A
Cuts at: 4026 4026
Size: 4850
BspMI ACCTGCnnnn'nnnn_
Cuts at: 207 316 680 1106 1274 1446 1900 2114 2416
Size: 109 364 426 168 172 454 214 302
Cuts at: 2416 2797 207
Size: 381 2260
Fragments arranged by size:
2260 454 426 381 364 302 214 172
168 109
BsrBI CCG'CTC
Cuts at: 50 1239 3142 3196 4680 4845 50
Size: 1189 1903 54 1484 165 55
Fragments arranged by size:
1903 1484 1189 165 55 54
BsrDI GCAATG_nn'
Cuts at: 2762 2762
Size: 4850
BsrFI r'CCGG_y
Cuts at: 840 1208 1271 1415 2848 3029 3238 840
Size: 368 63 144 1433 181 209 2452
Fragments arranged by size:
2452 1433 368 209 181 144 63
BssSI C'ACGA_G
Cuts at: 3121 3479 3121
Size: 358 4492
BstAPI GCAn_nnn'nTGC
Cuts at: 2227 2227
Size: 4850
BstBI TT'CG_AA
Cuts at: 3211 3211
Size: 4850
BstEII G'GTnAC_C
Cuts at: 189 1224 189
Size: 1035 3815
BstYI r'GATC_y
Cuts at: 1 1076 1875 2342 2365 2700 2946 3958 4044
Size: 1075 799 467 23 335 246 1012 86
Cuts at: 4044 4056 4402 1
Size: 12 346 449
Fragments arranged by size:
1075 1012 799 467 449 346 335 246
86 23 12
BtgI C'CryG_G
Cuts at: 1538 1538
Size: 4850
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 2800 2981 3144 2800
Size: 181 163 4506
Fragments arranged by size:
4506 181 163
BtsI GCAGTG_nn'
Cuts at: 1740 2093 1740
Size: 353 4497
DraI TTT'AAA
Cuts at: 1849 1849
Size: 4850
DrdI GACnn_nn'nnGTC
Cuts at: 1059 2556 3414 1059
Size: 1497 858 2495
Fragments arranged by size:
2495 1497 858
EaeI y'GGCC_r
Cuts at: 498 1055 1250 1448 1535 1541 1556 2435 2609
Size: 557 195 198 87 6 15 879 174
Cuts at: 2609 3000 3027 4372 498
Size: 391 27 1345 976
Fragments arranged by size:
1345 976 879 557 391 198 195 174
87 27 15 6
EagI C'GGCC_G
Cuts at: 498 1055 1250 1448 1556 2435 498
Size: 557 195 198 108 879 2913
Fragments arranged by size:
2913 879 557 198 195 108
EarI CTCTTCn'nnn_
Cuts at: 487 1193 2873 3083 487
Size: 706 1680 210 2254
Fragments arranged by size:
2254 1680 706 210
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 613 736 1113 3058 3368 3514 4660 613
Size: 123 377 1945 310 146 1146 803
Fragments arranged by size:
1945 1146 803 377 310 146 123
EcoICRI GAG'CTC
Cuts at: 1287 1473 1862 1287
Size: 186 389 4275
Fragments arranged by size:
4275 389 186
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 463 640 820 869 994 1005 1012 1399 1634
Size: 177 180 49 125 11 7 387 235
Cuts at: 1634 2675 3107 3854 4122 463
Size: 1041 432 747 268 1191
Fragments arranged by size:
1191 1041 747 432 387 268 235 180
177 125 49 11 7
EcoNI CCTnn'n_nnAGG
Cuts at: 373 373
Size: 4850
EcoO109I rG'GnC_Cy
Cuts at: 227 795 903 1032 1033 1492 1989 227
Size: 568 108 129 1 459 497 3088
Fragments arranged by size:
3088 568 497 459 129 108 1
EcoRI G'AATT_C
Cuts at: 1854 1854
Size: 4850
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2869 2869
Size: 4850
FspI TGC'GCA
Cuts at: 2631 2631
Size: 4850
HaeII r_GCGC'y
Cuts at: 832 1390 2284 2532 3554 4533 832
Size: 558 894 248 1022 979 1149
Fragments arranged by size:
1149 1022 979 894 558 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 370 402 978 1010 1511 1543 2692 2722 2724
Size: 32 576 32 501 32 1149 30 2
Cuts at: 2724 2754 370
Size: 30 2466
Fragments arranged by size:
2466 1149 576 501 32 32 32 30
30 2
HincII GTy'rAC
Cuts at: 1461 1519 1889 1461
Size: 58 370 4422
Fragments arranged by size:
4422 370 58
KasI G'GCGC_C
Cuts at: 1386 2280 2528 1386
Size: 894 248 3708
Fragments arranged by size:
3708 894 248
KpnI G_GTAC'C
Cuts at: 1870 1870
Size: 4850
MluI A'CGCG_T
Cuts at: 4283 4283
Size: 4850
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 563 3521 3705 563
Size: 2958 184 1708
Fragments arranged by size:
2958 1708 184
MscI TGG'CCA
Cuts at: 2611 2611
Size: 4850
MslI CAynn'nnrTG
Cuts at: 1069 2839 2966 1069
Size: 1770 127 2953
Fragments arranged by size:
2953 1770 127
MspA1I CmG'CkG
Cuts at: 383 636 1366 1607 1961 2275 2635 3648 3893
Size: 253 730 241 354 314 360 1013 245
Cuts at: 3893 383
Size: 1340
Fragments arranged by size:
1340 1013 730 360 354 314 253 245
241
NaeI GCC'GGC
Cuts at: 1210 3031 1210
Size: 1821 3029
NarI GG'CG_CC
Cuts at: 1387 2281 2529 1387
Size: 894 248 3708
Fragments arranged by size:
3708 894 248
NgoMIV G'CCGG_C
Cuts at: 1208 3029 1208
Size: 1821 3029
NspI r_CATG'y
Cuts at: 1903 2403 3310 1903
Size: 500 907 3443
Fragments arranged by size:
3443 907 500
PciI A'CATG_T
Cuts at: 3306 3306
Size: 4850
PfoI T'CCnGG_A
Cuts at: 165 617 165
Size: 452 4398
PmeI GTTT'AAAC
Cuts at: 1849 1849
Size: 4850
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 613 645 3989 4021 613
Size: 32 3344 32 1442
Fragments arranged by size:
3344 1442 32 32
PpuMI rG'GwC_Cy
Cuts at: 903 903
Size: 4850
PspOMI G'GGCC_C
Cuts at: 227 1032 1322 1491 227
Size: 805 290 169 3586
Fragments arranged by size:
3586 805 290 169
PspXI vC'TCGA_Gb
Cuts at: 1841 1841
Size: 4850
PstI C_TGCA'G
Cuts at: 719 755 859 1103 1897 2582 719
Size: 36 104 244 794 685 2987
Fragments arranged by size:
2987 794 685 244 104 36
PvuI CG_AT'CG
Cuts at: 104 104
Size: 4850
PvuII CAG'CTG
Cuts at: 2275 2635 2275
Size: 360 4490
RsrII CG'GwC_CG
Cuts at: 3045 3045
Size: 4850
SacI G_AGCT'C
Cuts at: 1289 1475 1864 1289
Size: 186 389 4275
Fragments arranged by size:
4275 389 186
SalI G'TCGA_C
Cuts at: 1517 1887 1517
Size: 370 4480
SanDI GG'GwC_CC
Cuts at: 903 903
Size: 4850
SapI GCTCTTCn'nnn_
Cuts at: 487 2873 3083 487
Size: 2386 210 2254
Fragments arranged by size:
2386 2254 210
SbfI CC_TGCA'GG
Cuts at: 1103 1897 1103
Size: 794 4056
ScaI AGT'ACT
Cuts at: 4367 4367
Size: 4850
SfcI C'TryA_G
Cuts at: 33 715 751 855 1099 1522 1893 2578 3571
Size: 682 36 104 244 423 371 685 993
Cuts at: 3571 3762 33
Size: 191 1121
Fragments arranged by size:
1121 993 685 682 423 371 244 191
104 36
SfiI GGCCn_nnn'nGGCC
Cuts at: 1680 1680
Size: 4850
SfoI GGC'GCC
Cuts at: 1388 2282 2530 1388
Size: 894 248 3708
Fragments arranged by size:
3708 894 248
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 4850
SmaI CCC'GGG
Cuts at: 174 232 1195 1872 174
Size: 58 963 677 3152
Fragments arranged by size:
3152 963 677 58
SmlI C'TyrA_G
Cuts at: 832 1474 1841 2005 2920 3412 3674 832
Size: 642 367 164 915 492 262 2008
Fragments arranged by size:
2008 915 642 492 367 262 164
SpeI A'CTAG_T
Cuts at: 67 67
Size: 4850
SphI G_CATG'C
Cuts at: 1903 2403 1903
Size: 500 4350
StuI AGG'CCT
Cuts at: 368 368
Size: 4850
StyI C'CwwG_G
Cuts at: 1984 2338 1984
Size: 354 4496
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 659 1243 1807 2433 2566 3025 3234 659
Size: 584 564 626 133 459 209 2275
Fragments arranged by size:
2275 626 584 564 459 209 133
TatI w'GTAC_w
Cuts at: 302 650 4365 302
Size: 348 3715 787
Fragments arranged by size:
3715 787 348
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 800 832 1749 1781 4367 4399 800
Size: 32 917 32 2586 32 1251
Fragments arranged by size:
2586 1251 917 32 32 32
Tth111I GACn'n_nGTC
Cuts at: 2647 2647
Size: 4850
XbaI T'CTAG_A
Cuts at: 1881 1881
Size: 4850
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2345 2345
Size: 4850
XhoI C'TCGA_G
Cuts at: 1841 1841
Size: 4850
XmaI C'CCGG_G
Cuts at: 172 230 1193 1870 172
Size: 58 963 677 3152
Fragments arranged by size:
3152 963 677 58
Enzymes that do cut:
AarI AccI Acc65I AclI AcuI AfeI AflII AflIII
AgeI AlfI AloI AlwNI ApaI ApaLI ApoI AseI
AsiSI AvaI BamHI BanI BanII BbeI BciVI BclI
BglI BglII BlpI Bme1580I BmrI BplI BpmI BpuEI
BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI
BsgI BsiEI BsiHKAI BsmBI Bsp1286I BspEI BspHI BspMI
BsrBI BsrDI BsrFI BssSI BstAPI BstBI BstEII BstYI
BtgI BtgZI BtsI DraI DrdI EaeI EagI EarI
EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI FalI FspI
HaeII Hin4I HincII KasI KpnI MluI MmeI MscI
MslI MspA1I NaeI NarI NgoMIV NspI PciI PfoI
PmeI PpiI PpuMI PspOMI PspXI PstI PvuI PvuII
RsrII SacI SalI SanDI SapI SbfI ScaI SfcI
SfiI SfoI SgfI SmaI SmlI SpeI SphI StuI
StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI
XmaI
Enzymes that do not cut:
AatII AhdI AleI AscI AvrII BaeI BbsI BbvCI
BcgI BfrBI BmgBI BmtI Bpu10I BsiWI BsmI BsrGI
BssHII BstXI BstZ17I Bsu36I ClaI CspCI DraIII EcoRV
FseI FspAI HindIII HpaI MfeI NcoI NdeI NheI
NotI NruI NsiI PacI PasI PflMI PmlI PshAI
PsiI PsrI SacII SexAI SgrAI SnaBI SrfI SspI
SwaI XmnI ZraI