Restriction map for
pF1KE0004
(Circular) (Six-base) MAPSORT of: pF1KE0004.seq Check: 6917 from: 1 to: 4571
pF1KE0004 4571 bp
With 182 enzymes: SgfI *
April 24, 2009 13:09 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 290 290
Size: 4571
AccI GT'mk_AC
Cuts at: 1609 1609
Size: 4571
Acc65I G'GTAC_C
Cuts at: 1587 1587
Size: 4571
AclI AA'CG_TT
Cuts at: 4411 4411
Size: 4571
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 373 581 1330 1401 1498 2396 2828 3575 3843
Size: 208 749 71 97 898 432 747 268
Cuts at: 3843 373
Size: 1101
Fragments arranged by size:
1101 898 749 747 432 268 208 97
71
AflIII A'CryG_T
Cuts at: 410 838 1397 1923 3027 4004 410
Size: 428 559 526 1104 977 977
Fragments arranged by size:
1104 977 977 559 526 428
AgeI A'CCGG_T
Cuts at: 2959 2959
Size: 4571
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 550 582 550
Size: 32 4539
AlwNI CAG_nnn'CTG
Cuts at: 1876 3443 1876
Size: 1567 3004
ApaI G_GGCC'C
Cuts at: 1378 1378
Size: 4571
ApaLI G'TGCA_C
Cuts at: 3341 3341
Size: 4571
ApoI r'AATT_y
Cuts at: 1575 3924 4180 1575
Size: 2349 256 1966
Fragments arranged by size:
2349 1966 256
AseI AT'TA_AT
Cuts at: 20 20
Size: 4571
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 4571
AvaI C'yCGr_G
Cuts at: 289 576 1591 289
Size: 287 1015 3269
Fragments arranged by size:
3269 1015 287
AvrII C'CTAG_G
Cuts at: 1185 1185
Size: 4571
BamHI G'GATC_C
Cuts at: 902 1596 902
Size: 694 3877
BanI G'GyrC_C
Cuts at: 1036 1048 1587 2001 2249 2284 1036
Size: 12 539 414 248 35 3323
Fragments arranged by size:
3323 539 414 248 35 12
BanII G_rGCy'C
Cuts at: 437 1378 1433 1510 1585 2615 437
Size: 941 55 77 75 1030 2393
Fragments arranged by size:
2393 1030 941 77 75 55
BbeI G_GCGC'C
Cuts at: 2005 2253 2005
Size: 248 4323
BciVI GTATCCnnnnn_n'
Cuts at: 2469 3230 4571 2469
Size: 761 1341 2469
Fragments arranged by size:
2469 1341 761
BclI T'GATC_A
Cuts at: 678 968 2091 678
Size: 290 1123 3158
Fragments arranged by size:
3158 1123 290
BglII A'GATC_T
Cuts at: 2086 4123 2086
Size: 2037 2534
BlpI GC'TnA_GC
Cuts at: 1683 1683
Size: 4571
Bme1580I G_kGCm'C
Cuts at: 1051 1378 2196 2289 3345 1051
Size: 327 818 93 1056 2277
Fragments arranged by size:
2277 1056 818 327 93
BmrI ACTGGGnnnn_n'
Cuts at: 204 440 1783 1864 2199 4343 204
Size: 236 1343 81 335 2144 432
Fragments arranged by size:
2144 1343 432 335 236 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2938 2938
Size: 4571
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 382 763 958 1417 382
Size: 381 195 459 3536
Fragments arranged by size:
3536 459 381 195
Bpu10I CC'TnA_GC
Cuts at: 1504 1504
Size: 4571
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1747 2626 3118 3416 1747
Size: 879 492 298 2902
Fragments arranged by size:
2902 879 492 298
BsaI GGTCTCn'nnnn_
Cuts at: 4275 4275
Size: 4571
BsaAI yAC'GTr
Cuts at: 413 1924 413
Size: 1511 3060
BsaBI GATnn'nnATC
Cuts at: 6 2085 4122 6
Size: 2079 2037 455
Fragments arranged by size:
2079 2037 455
BsaHI Gr'CG_yC
Cuts at: 730 2002 2250 2952 4448 730
Size: 1272 248 702 1496 853
Fragments arranged by size:
1496 1272 853 702 248
BsaWI w'CCGG_w
Cuts at: 563 1218 1760 2281 2959 3233 3380 3791 563
Size: 655 542 521 678 274 147 411 1343
Fragments arranged by size:
1343 678 655 542 521 411 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 308 338 644 674 308
Size: 30 306 30 4205
Fragments arranged by size:
4205 306 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 191 539 191
Size: 348 4223
BseYI C'CCAG_C
Cuts at: 1293 1996 2754 3331 3827 1293
Size: 703 758 577 496 2037
Fragments arranged by size:
2037 758 703 577 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 658 658
Size: 4571
BsiEI CG_ry'CG
Cuts at: 104 2113 2159 3367 104
Size: 2009 46 1208 1308
Fragments arranged by size:
2009 1308 1208 46
BsiHKAI G_wGCw'C
Cuts at: 296 1585 2363 2553 3345 296
Size: 1289 778 190 792 1522
Fragments arranged by size:
1522 1289 792 778 190
BsmI GAATG_Cn'
Cuts at: 961 961
Size: 4571
Bsp1286I G_dGCh'C
Cuts at: 296 437 1051 1378 1433 1510 1585 2196 2289
Size: 141 614 327 55 77 75 611 93
Cuts at: 2289 2363 2553 2615 3345 296
Size: 74 190 62 730 1522
Fragments arranged by size:
1522 730 614 611 327 190 141 93
77 75 74 62 55
BspEI T'CCGG_A
Cuts at: 3791 3791
Size: 4571
BspHI T'CATG_A
Cuts at: 3747 3747
Size: 4571
BspMI ACCTGCnnnn'nnnn_
Cuts at: 290 1475 1621 1835 2137 2518 290
Size: 1185 146 214 302 381 2343
Fragments arranged by size:
2343 1185 381 302 214 146
BsrBI CCG'CTC
Cuts at: 50 2863 2917 4401 4566 50
Size: 2813 54 1484 165 55
Fragments arranged by size:
2813 1484 165 55 54
BsrDI GCAATG_nn'
Cuts at: 466 2483 466
Size: 2017 2554
BsrFI r'CCGG_y
Cuts at: 1330 2569 2750 2959 1330
Size: 1239 181 209 2942
Fragments arranged by size:
2942 1239 209 181
BssSI C'ACGA_G
Cuts at: 2842 3200 2842
Size: 358 4213
BstAPI GCAn_nnn'nTGC
Cuts at: 1948 1948
Size: 4571
BstBI TT'CG_AA
Cuts at: 2932 2932
Size: 4571
BstYI r'GATC_y
Cuts at: 1 902 1544 1596 2063 2086 2421 2667 3679
Size: 901 642 52 467 23 335 246 1012
Cuts at: 3679 3765 3777 4123 1
Size: 86 12 346 449
Fragments arranged by size:
1012 901 642 467 449 346 335 246
86 52 23 12
Bsu36I CC'TnA_GG
Cuts at: 1285 1285
Size: 4571
BtgI C'CryG_G
Cuts at: 617 617
Size: 4571
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 2521 2702 2865 2521
Size: 181 163 4227
Fragments arranged by size:
4227 181 163
BtsI GCAGTG_nn'
Cuts at: 1095 1814 1095
Size: 719 3852
DraI TTT'AAA
Cuts at: 1570 1570
Size: 4571
DrdI GACnn_nn'nnGTC
Cuts at: 2277 3135 2277
Size: 858 3713
EaeI y'GGCC_r
Cuts at: 533 2156 2330 2721 2748 4093 533
Size: 1623 174 391 27 1345 1011
Fragments arranged by size:
1623 1345 1011 391 174 27
EagI C'GGCC_G
Cuts at: 2156 2156
Size: 4571
EarI CTCTTCn'nnn_
Cuts at: 855 2594 2804 855
Size: 1739 210 2622
Fragments arranged by size:
2622 1739 210
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 2779 3089 3235 4381 2779
Size: 310 146 1146 2969
Fragments arranged by size:
2969 1146 310 146
EcoICRI GAG'CTC
Cuts at: 1583 1583
Size: 4571
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 373 382 581 763 958 1330 1401 1417 1498
Size: 9 199 182 195 372 71 16 81
Cuts at: 1498 2396 2828 3575 3843 373
Size: 898 432 747 268 1101
Fragments arranged by size:
1101 898 747 432 372 268 199 195
182 81 71 16 9
EcoO109I rG'GnC_Cy
Cuts at: 144 251 1290 1375 1440 1710 144
Size: 107 1039 85 65 270 3005
Fragments arranged by size:
3005 1039 270 107 85 65
EcoRI G'AATT_C
Cuts at: 1575 1575
Size: 4571
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2590 2590
Size: 4571
FspI TGC'GCA
Cuts at: 995 2352 995
Size: 1357 3214
HaeII r_GCGC'y
Cuts at: 1325 2005 2253 3275 4254 1325
Size: 680 248 1022 979 1642
Fragments arranged by size:
1642 1022 979 680 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1529 1561 2413 2443 2445 2475 1529
Size: 32 852 30 2 30 3625
Fragments arranged by size:
3625 852 32 30 30 2
HincII GTy'rAC
Cuts at: 869 1395 1610 869
Size: 526 215 3830
Fragments arranged by size:
3830 526 215
KasI G'GCGC_C
Cuts at: 2001 2249 2001
Size: 248 4323
KpnI G_GTAC'C
Cuts at: 1591 1591
Size: 4571
MluI A'CGCG_T
Cuts at: 4004 4004
Size: 4571
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 999 3242 3426 999
Size: 2243 184 2144
Fragments arranged by size:
2243 2144 184
MscI TGG'CCA
Cuts at: 2332 2332
Size: 4571
MslI CAynn'nnrTG
Cuts at: 299 2560 2687 299
Size: 2261 127 2183
Fragments arranged by size:
2261 2183 127
MspA1I CmG'CkG
Cuts at: 1682 1996 2356 3369 3614 1682
Size: 314 360 1013 245 2639
Fragments arranged by size:
2639 1013 360 314 245
NaeI GCC'GGC
Cuts at: 1332 2752 1332
Size: 1420 3151
NarI GG'CG_CC
Cuts at: 2002 2250 2002
Size: 248 4323
NcoI C'CATG_G
Cuts at: 617 617
Size: 4571
NgoMIV G'CCGG_C
Cuts at: 1330 2750 1330
Size: 1420 3151
NspI r_CATG'y
Cuts at: 867 1401 1624 2124 3031 867
Size: 534 223 500 907 2407
Fragments arranged by size:
2407 907 534 500 223
PasI CC'CwG_GG
Cuts at: 1104 1104
Size: 4571
PciI A'CATG_T
Cuts at: 1397 3027 1397
Size: 1630 2941
PflMI CCAn_nnn'nTGG
Cuts at: 807 951 807
Size: 144 4427
PfoI T'CCnGG_A
Cuts at: 1256 1547 1256
Size: 291 4280
PmeI GTTT'AAAC
Cuts at: 1570 1570
Size: 4571
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3710 3742 3710
Size: 32 4539
PpuMI rG'GwC_Cy
Cuts at: 251 1290 251
Size: 1039 3532
PspOMI G'GGCC_C
Cuts at: 1374 1374
Size: 4571
PstI C_TGCA'G
Cuts at: 615 1046 1618 2303 615
Size: 431 572 685 2883
Fragments arranged by size:
2883 685 572 431
PvuI CG_AT'CG
Cuts at: 104 104
Size: 4571
PvuII CAG'CTG
Cuts at: 1996 2356 1996
Size: 360 4211
RsrII CG'GwC_CG
Cuts at: 2766 2766
Size: 4571
SacI G_AGCT'C
Cuts at: 1585 1585
Size: 4571
SalI G'TCGA_C
Cuts at: 1608 1608
Size: 4571
SanDI GG'GwC_CC
Cuts at: 1290 1290
Size: 4571
SapI GCTCTTCn'nnn_
Cuts at: 2594 2804 2594
Size: 210 4361
SbfI CC_TGCA'GG
Cuts at: 1046 1618 1046
Size: 572 3999
ScaI AGT'ACT
Cuts at: 4088 4088
Size: 4571
SfcI C'TryA_G
Cuts at: 33 611 1042 1460 1614 2299 3292 3483 33
Size: 578 431 418 154 685 993 191 1121
Fragments arranged by size:
1121 993 685 578 431 418 191 154
SfoI GGC'GCC
Cuts at: 2003 2251 2003
Size: 248 4323
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 4571
SmaI CCC'GGG
Cuts at: 1593 1593
Size: 4571
SmlI C'TyrA_G
Cuts at: 1726 2641 3133 3395 1726
Size: 915 492 262 2902
Fragments arranged by size:
2902 915 492 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 4571
SphI G_CATG'C
Cuts at: 1624 2124 1624
Size: 500 4071
StuI AGG'CCT
Cuts at: 424 1115 424
Size: 691 3880
StyI C'CwwG_G
Cuts at: 617 806 1185 1705 2059 617
Size: 189 379 520 354 3129
Fragments arranged by size:
3129 520 379 354 189
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 2154 2287 2746 2955 2154
Size: 133 459 209 3770
Fragments arranged by size:
3770 459 209 133
TatI w'GTAC_w
Cuts at: 1400 4086 1400
Size: 2686 1885
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 308 340 1252 1284 4088 4120 308
Size: 32 912 32 2804 32 759
Fragments arranged by size:
2804 912 759 32 32 32
Tth111I GACn'n_nGTC
Cuts at: 2368 2368
Size: 4571
XbaI T'CTAG_A
Cuts at: 1602 1602
Size: 4571
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 1059 1441 2066 1059
Size: 382 625 3564
Fragments arranged by size:
3564 625 382
XmaI C'CCGG_G
Cuts at: 1591 1591
Size: 4571
Enzymes that do cut:
AarI AccI Acc65I AclI AcuI AflIII AgeI AloI
AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI AvrII
BamHI BanI BanII BbeI BciVI BclI BglII BlpI
Bme1580I BmrI BplI BpmI Bpu10I BpuEI BsaI BsaAI
BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI
BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI
BsrFI BssSI BstAPI BstBI BstYI Bsu36I BtgI BtgZI
BtsI DraI DrdI EaeI EagI EarI EciI EcoICRI
Eco57MI EcoO109I EcoRI FalI FspI HaeII Hin4I HincII
KasI KpnI MluI MmeI MscI MslI MspA1I NaeI
NarI NcoI NgoMIV NspI PasI PciI PflMI PfoI
PmeI PpiI PpuMI PspOMI PstI PvuI PvuII RsrII
SacI SalI SanDI SapI SbfI ScaI SfcI SfoI
SgfI SmaI SmlI SpeI SphI StuI StyI TaqII
TatI TstI Tth111I XbaI XcmI XmaI
Enzymes that do not cut:
AatII AfeI AflII AhdI AleI AlfI AscI BaeI
BbsI BbvCI BcgI BfrBI BglI BmgBI BmtI BsiWI
BsmBI BsrGI BssHII BstEII BstXI BstZ17I ClaI CspCI
DraIII EcoNI EcoRV FseI FspAI HindIII HpaI MfeI
NdeI NheI NotI NruI NsiI PacI PmlI PshAI
PsiI PspXI PsrI SacII SexAI SfiI SgrAI SnaBI
SrfI SspI SwaI XhoI XmnI ZraI