Restriction map for
pF1KE0005
(Circular) (Six-base) MAPSORT of: pF1KE0005.seq Check: 3414 from: 1 to: 5216
pF1KE0005 5216 bp
With 182 enzymes: SgfI *
April 24, 2009 13:09 ..
AccI GT'mk_AC
Cuts at: 2254 2254
Size: 5216
Acc65I G'GTAC_C
Cuts at: 1946 2232 1946
Size: 286 4930
AclI AA'CG_TT
Cuts at: 5056 5056
Size: 5216
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 761 1477 1868 2206 3041 3473 4220 4488 761
Size: 716 391 338 835 432 747 268 1489
Fragments arranged by size:
1489 835 747 716 432 391 338 268
AflIII A'CryG_T
Cuts at: 1981 2568 3672 4649 1981
Size: 587 1104 977 2548
Fragments arranged by size:
2548 1104 977 587
AgeI A'CCGG_T
Cuts at: 3604 3604
Size: 5216
AleI CACnn'nnGTG
Cuts at: 965 965
Size: 5216
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 1409 1441 1409
Size: 32 5184
AlwNI CAG_nnn'CTG
Cuts at: 2521 4088 2521
Size: 1567 3649
ApaLI G'TGCA_C
Cuts at: 3986 3986
Size: 5216
ApoI r'AATT_y
Cuts at: 586 652 789 796 1299 1585 1718 1815 1832
Size: 66 137 7 503 286 133 97 17
Cuts at: 1832 2220 4569 4825 586
Size: 388 2349 256 977
Fragments arranged by size:
2349 977 503 388 286 256 137 133
97 66 17 7
AseI AT'TA_AT
Cuts at: 20 20
Size: 5216
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 5216
AvaI C'yCGr_G
Cuts at: 2236 2236
Size: 5216
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'
Cuts at: 425 458 425
Size: 33 5183
BamHI G'GATC_C
Cuts at: 2241 2241
Size: 5216
BanI G'GyrC_C
Cuts at: 1946 2232 2646 2894 2929 1946
Size: 286 414 248 35 4233
Fragments arranged by size:
4233 414 286 248 35
BanII G_rGCy'C
Cuts at: 2230 3260 2230
Size: 1030 4186
BbeI G_GCGC'C
Cuts at: 2650 2898 2650
Size: 248 4968
BbsI GAAGACnn'nnnn_
Cuts at: 1508 1805 1508
Size: 297 4919
BciVI GTATCCnnnnn_n'
Cuts at: 1663 1900 3114 3875 5216 1663
Size: 237 1214 761 1341 1663
Fragments arranged by size:
1663 1341 1214 761 237
BclI T'GATC_A
Cuts at: 162 2736 162
Size: 2574 2642
BglII A'GATC_T
Cuts at: 327 2731 4768 327
Size: 2404 2037 775
Fragments arranged by size:
2404 2037 775
BlpI GC'TnA_GC
Cuts at: 2328 2328
Size: 5216
Bme1580I G_kGCm'C
Cuts at: 2841 2934 3990 2841
Size: 93 1056 4067
Fragments arranged by size:
4067 1056 93
BmrI ACTGGGnnnn_n'
Cuts at: 2428 2509 2844 4988 2428
Size: 81 335 2144 2656
Fragments arranged by size:
2656 2144 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3583 3583
Size: 5216
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1154 1154
Size: 5216
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 313 2392 3271 3763 4061 313
Size: 2079 879 492 298 1468
Fragments arranged by size:
2079 1468 879 492 298
BsaI GGTCTCn'nnnn_
Cuts at: 4920 4920
Size: 5216
BsaAI yAC'GTr
Cuts at: 2569 2569
Size: 5216
BsaBI GATnn'nnATC
Cuts at: 6 2730 4767 6
Size: 2724 2037 455
Fragments arranged by size:
2724 2037 455
BsaHI Gr'CG_yC
Cuts at: 2647 2895 3597 5093 2647
Size: 248 702 1496 2770
Fragments arranged by size:
2770 1496 702 248
BsaWI w'CCGG_w
Cuts at: 2405 2926 3604 3878 4025 4436 2405
Size: 521 678 274 147 411 3185
Fragments arranged by size:
3185 678 521 411 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1751 1781 1751
Size: 30 5186
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1867 1867
Size: 5216
BseYI C'CCAG_C
Cuts at: 2641 3399 3976 4472 2641
Size: 758 577 496 3385
Fragments arranged by size:
3385 758 577 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1792 1792
Size: 5216
BsiEI CG_ry'CG
Cuts at: 104 2758 2804 4012 104
Size: 2654 46 1208 1308
Fragments arranged by size:
2654 1308 1208 46
BsiHKAI G_wGCw'C
Cuts at: 2230 3008 3198 3990 2230
Size: 778 190 792 3456
Fragments arranged by size:
3456 792 778 190
BsmI GAATG_Cn'
Cuts at: 994 994
Size: 5216
Bsp1286I G_dGCh'C
Cuts at: 2230 2841 2934 3008 3198 3260 3990 2230
Size: 611 93 74 190 62 730 3456
Fragments arranged by size:
3456 730 611 190 93 74 62
BspEI T'CCGG_A
Cuts at: 4436 4436
Size: 5216
BspHI T'CATG_A
Cuts at: 507 2082 4392 507
Size: 1575 2310 1331
Fragments arranged by size:
2310 1575 1331
BspMI ACCTGCnnnn'nnnn_
Cuts at: 2266 2480 2782 3163 2266
Size: 214 302 381 4319
Fragments arranged by size:
4319 381 302 214
BsrBI CCG'CTC
Cuts at: 50 3508 3562 5046 5211 50
Size: 3458 54 1484 165 55
Fragments arranged by size:
3458 1484 165 55 54
BsrDI GCAATG_nn'
Cuts at: 1154 1618 3128 1154
Size: 464 1510 3242
Fragments arranged by size:
3242 1510 464
BsrFI r'CCGG_y
Cuts at: 3214 3395 3604 3214
Size: 181 209 4826
Fragments arranged by size:
4826 209 181
BssSI C'ACGA_G
Cuts at: 3487 3845 3487
Size: 358 4858
BstAPI GCAn_nnn'nTGC
Cuts at: 2593 2593
Size: 5216
BstBI TT'CG_AA
Cuts at: 3577 3577
Size: 5216
BstEII G'GTnAC_C
Cuts at: 397 397
Size: 5216
BstYI r'GATC_y
Cuts at: 1 327 2241 2708 2731 3066 3312 4324 4410
Size: 326 1914 467 23 335 246 1012 86
Cuts at: 4410 4422 4768 1
Size: 12 346 449
Fragments arranged by size:
1914 1012 467 449 346 335 326 246
86 23 12
Bsu36I CC'TnA_GG
Cuts at: 1333 1333
Size: 5216
BtgI C'CryG_G
Cuts at: 107 107
Size: 5216
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 3166 3347 3510 3166
Size: 181 163 4872
Fragments arranged by size:
4872 181 163
BtsI GCAGTG_nn'
Cuts at: 915 1948 2459 915
Size: 1033 511 3672
Fragments arranged by size:
3672 1033 511
ClaI AT'CG_AT
Cuts at: 257 257
Size: 5216
DraI TTT'AAA
Cuts at: 765 807 1304 1992 2215 765
Size: 42 497 688 223 3766
Fragments arranged by size:
3766 688 497 223 42
DraIII CAC_nnn'GTG
Cuts at: 202 202
Size: 5216
DrdI GACnn_nn'nnGTC
Cuts at: 2092 2922 3780 2092
Size: 830 858 3528
Fragments arranged by size:
3528 858 830
EaeI y'GGCC_r
Cuts at: 2801 2975 3366 3393 4738 2801
Size: 174 391 27 1345 3279
Fragments arranged by size:
3279 1345 391 174 27
EagI C'GGCC_G
Cuts at: 2801 2801
Size: 5216
EarI CTCTTCn'nnn_
Cuts at: 737 1844 3239 3449 737
Size: 1107 1395 210 2504
Fragments arranged by size:
2504 1395 1107 210
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 3424 3734 3880 5026 3424
Size: 310 146 1146 3614
Fragments arranged by size:
3614 1146 310 146
EcoICRI GAG'CTC
Cuts at: 2228 2228
Size: 5216
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 761 1154 1477 1868 2206 3041 3473 4220 4488
Size: 393 323 391 338 835 432 747 268
Cuts at: 4488 761
Size: 1489
Fragments arranged by size:
1489 835 747 432 393 391 338 323
268
EcoNI CCTnn'n_nnAGG
Cuts at: 1030 1030
Size: 5216
EcoO109I rG'GnC_Cy
Cuts at: 2355 2355
Size: 5216
EcoRI G'AATT_C
Cuts at: 586 1585 2220 586
Size: 999 635 3582
Fragments arranged by size:
3582 999 635
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 1585 3235 1585
Size: 1650 3566
FspI TGC'GCA
Cuts at: 1687 2997 1687
Size: 1310 3906
FspAI rTGC'GCAy
Cuts at: 1687 1687
Size: 5216
HaeII r_GCGC'y
Cuts at: 2650 2898 3920 4899 2650
Size: 248 1022 979 2967
Fragments arranged by size:
2967 1022 979 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1245 1277 1720 1752 1843 1875 3058 3088 3090
Size: 32 443 32 91 32 1183 30 2
Cuts at: 3090 3120 1245
Size: 30 3341
Fragments arranged by size:
3341 1183 443 91 32 32 32 30
30 2
HincII GTy'rAC
Cuts at: 855 2255 855
Size: 1400 3816
KasI G'GCGC_C
Cuts at: 2646 2894 2646
Size: 248 4968
KpnI G_GTAC'C
Cuts at: 1950 2236 1950
Size: 286 4930
MluI A'CGCG_T
Cuts at: 4649 4649
Size: 5216
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 150 1592 3887 4071 150
Size: 1442 2295 184 1295
Fragments arranged by size:
2295 1442 1295 184
MscI TGG'CCA
Cuts at: 2977 2977
Size: 5216
MslI CAynn'nnrTG
Cuts at: 965 2117 3205 3332 965
Size: 1152 1088 127 2849
Fragments arranged by size:
2849 1152 1088 127
MspA1I CmG'CkG
Cuts at: 2327 2641 3001 4014 4259 2327
Size: 314 360 1013 245 3284
Fragments arranged by size:
3284 1013 360 314 245
NaeI GCC'GGC
Cuts at: 3397 3397
Size: 5216
NarI GG'CG_CC
Cuts at: 2647 2895 2647
Size: 248 4968
NcoI C'CATG_G
Cuts at: 107 107
Size: 5216
NgoMIV G'CCGG_C
Cuts at: 3395 3395
Size: 5216
NspI r_CATG'y
Cuts at: 1985 2269 2769 3676 1985
Size: 284 500 907 3525
Fragments arranged by size:
3525 907 500 284
PciI A'CATG_T
Cuts at: 1981 3672 1981
Size: 1691 3525
PflMI CCAn_nnn'nTGG
Cuts at: 1903 2073 1903
Size: 170 5046
PmeI GTTT'AAAC
Cuts at: 2215 2215
Size: 5216
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4355 4387 4355
Size: 32 5184
PstI C_TGCA'G
Cuts at: 2263 2948 2263
Size: 685 4531
PvuI CG_AT'CG
Cuts at: 104 104
Size: 5216
PvuII CAG'CTG
Cuts at: 2641 3001 2641
Size: 360 4856
RsrII CG'GwC_CG
Cuts at: 3411 3411
Size: 5216
SacI G_AGCT'C
Cuts at: 2230 2230
Size: 5216
SalI G'TCGA_C
Cuts at: 2253 2253
Size: 5216
SapI GCTCTTCn'nnn_
Cuts at: 3239 3449 3239
Size: 210 5006
SbfI CC_TGCA'GG
Cuts at: 2263 2263
Size: 5216
ScaI AGT'ACT
Cuts at: 1901 4733 1901
Size: 2832 2384
SexAI A'CCwGG_T
Cuts at: 1838 2019 1838
Size: 181 5035
SfcI C'TryA_G
Cuts at: 33 671 1238 1975 2028 2259 2944 3937 4128
Size: 638 567 737 53 231 685 993 191
Cuts at: 4128 33
Size: 1121
Fragments arranged by size:
1121 993 737 685 638 567 231 191
53
SfoI GGC'GCC
Cuts at: 2648 2896 2648
Size: 248 4968
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 5216
SmaI CCC'GGG
Cuts at: 2238 2238
Size: 5216
SmlI C'TyrA_G
Cuts at: 292 2371 3286 3778 4040 292
Size: 2079 915 492 262 1468
Fragments arranged by size:
2079 1468 915 492 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 5216
SphI G_CATG'C
Cuts at: 2269 2769 2269
Size: 500 4716
SspI AAT'ATT
Cuts at: 769 1188 1230 1914 769
Size: 419 42 684 4071
Fragments arranged by size:
4071 684 419 42
StuI AGG'CCT
Cuts at: 978 1115 978
Size: 137 5079
StyI C'CwwG_G
Cuts at: 107 2350 2704 107
Size: 2243 354 2619
Fragments arranged by size:
2619 2243 354
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 1223 1654 2799 2932 3391 3600 1223
Size: 431 1145 133 459 209 2839
Fragments arranged by size:
2839 1145 459 431 209 133
TatI w'GTAC_w
Cuts at: 386 1899 4731 386
Size: 1513 2832 871
Fragments arranged by size:
2832 1513 871
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 860 892 4733 4765 860
Size: 32 3841 32 1311
Fragments arranged by size:
3841 1311 32 32
Tth111I GACn'n_nGTC
Cuts at: 1259 3013 1259
Size: 1754 3462
XbaI T'CTAG_A
Cuts at: 2247 2247
Size: 5216
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2711 2711
Size: 5216
XmaI C'CCGG_G
Cuts at: 2236 2236
Size: 5216
Enzymes that do cut:
AccI Acc65I AclI AcuI AflIII AgeI AleI AloI
AlwNI ApaLI ApoI AseI AsiSI AvaI BaeI BamHI
BanI BanII BbeI BbsI BciVI BclI BglII BlpI
Bme1580I BmrI BplI BpmI BpuEI BsaI BsaAI BsaBI
BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI
BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI
BssSI BstAPI BstBI BstEII BstYI Bsu36I BtgI BtgZI
BtsI ClaI DraI DraIII DrdI EaeI EagI EarI
EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI FalI FspI
FspAI HaeII Hin4I HincII KasI KpnI MluI MmeI
MscI MslI MspA1I NaeI NarI NcoI NgoMIV NspI
PciI PflMI PmeI PpiI PstI PvuI PvuII RsrII
SacI SalI SapI SbfI ScaI SexAI SfcI SfoI
SgfI SmaI SmlI SpeI SphI SspI StuI StyI
TaqII TatI TstI Tth111I XbaI XcmI XmaI
Enzymes that do not cut:
AarI AatII AfeI AflII AhdI AlfI ApaI AscI
AvrII BbvCI BcgI BfrBI BglI BmgBI BmtI Bpu10I
BsiWI BsmBI BsrGI BssHII BstXI BstZ17I CspCI EcoRV
FseI HindIII HpaI MfeI NdeI NheI NotI NruI
NsiI PacI PasI PfoI PmlI PpuMI PshAI PsiI
PspOMI PspXI PsrI SacII SanDI SfiI SgrAI SnaBI
SrfI SwaI XhoI XmnI ZraI