Restriction map for
pF1KE0006
(Circular) (Six-base) MAPSORT of: pF1KE0006.seq Check: 7554 from: 1 to: 4676
pF1KE0006 4676 bp
With 182 enzymes: SgfI *
April 24, 2009 13:09 ..
AccI GT'mk_AC
Cuts at: 179 1559 1714 179
Size: 1380 155 3141
Fragments arranged by size:
3141 1380 155
Acc65I G'GTAC_C
Cuts at: 1692 1692
Size: 4676
AclI AA'CG_TT
Cuts at: 4516 4516
Size: 4676
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 2501 2933 3680 3948 2501
Size: 432 747 268 3229
Fragments arranged by size:
3229 747 432 268
AfeI AGC'GCT
Cuts at: 324 324
Size: 4676
AflIII A'CryG_T
Cuts at: 2028 3132 4109 2028
Size: 1104 977 2595
Fragments arranged by size:
2595 1104 977
AgeI A'CCGG_T
Cuts at: 3064 3064
Size: 4676
AleI CACnn'nnGTG
Cuts at: 1210 1210
Size: 4676
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 701 929 701
Size: 228 4448
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 817 849 926 958 817
Size: 32 77 32 4535
Fragments arranged by size:
4535 77 32 32
AlwNI CAG_nnn'CTG
Cuts at: 1047 1981 3548 1047
Size: 934 1567 2175
Fragments arranged by size:
2175 1567 934
ApaLI G'TGCA_C
Cuts at: 3446 3446
Size: 4676
ApoI r'AATT_y
Cuts at: 214 265 1249 1353 1680 4029 4285 214
Size: 51 984 104 327 2349 256 605
Fragments arranged by size:
2349 984 605 327 256 104 51
AseI AT'TA_AT
Cuts at: 20 572 20
Size: 552 4124
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 4676
AvaI C'yCGr_G
Cuts at: 762 1696 762
Size: 934 3742
BamHI G'GATC_C
Cuts at: 941 1701 941
Size: 760 3916
BanI G'GyrC_C
Cuts at: 1692 2106 2354 2389 1692
Size: 414 248 35 3979
Fragments arranged by size:
3979 414 248 35
BanII G_rGCy'C
Cuts at: 1690 2720 1690
Size: 1030 3646
BbeI G_GCGC'C
Cuts at: 2110 2358 2110
Size: 248 4428
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1156 1190 1156
Size: 34 4642
BciVI GTATCCnnnnn_n'
Cuts at: 2574 3335 4676 2574
Size: 761 1341 2574
Fragments arranged by size:
2574 1341 761
BclI T'GATC_A
Cuts at: 2196 2196
Size: 4676
BglII A'GATC_T
Cuts at: 2191 4228 2191
Size: 2037 2639
BlpI GC'TnA_GC
Cuts at: 1788 1788
Size: 4676
Bme1580I G_kGCm'C
Cuts at: 2301 2394 3450 2301
Size: 93 1056 3527
Fragments arranged by size:
3527 1056 93
BmrI ACTGGGnnnn_n'
Cuts at: 1888 1969 2304 4448 1888
Size: 81 335 2144 2116
Fragments arranged by size:
2144 2116 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 341 3043 341
Size: 2702 1974
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 169 169
Size: 4676
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 805 1099 1852 2731 3223 3521 805
Size: 294 753 879 492 298 1960
Fragments arranged by size:
1960 879 753 492 298 294
BsaI GGTCTCn'nnnn_
Cuts at: 692 774 4380 692
Size: 82 3606 988
Fragments arranged by size:
3606 988 82
BsaAI yAC'GTr
Cuts at: 2029 2029
Size: 4676
BsaBI GATnn'nnATC
Cuts at: 6 1382 2190 4227 6
Size: 1376 808 2037 455
Fragments arranged by size:
2037 1376 808 455
BsaHI Gr'CG_yC
Cuts at: 2107 2355 3057 4553 2107
Size: 248 702 1496 2230
Fragments arranged by size:
2230 1496 702 248
BsaWI w'CCGG_w
Cuts at: 1865 2386 3064 3338 3485 3896 1865
Size: 521 678 274 147 411 2645
Fragments arranged by size:
2645 678 521 411 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1094 1124 1094
Size: 30 4646
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 166 333 1102 166
Size: 167 769 3740
Fragments arranged by size:
3740 769 167
BseYI C'CCAG_C
Cuts at: 302 674 2101 2859 3436 3932 302
Size: 372 1427 758 577 496 1046
Fragments arranged by size:
1427 1046 758 577 496 372
BsiEI CG_ry'CG
Cuts at: 104 2218 2264 3472 104
Size: 2114 46 1208 1308
Fragments arranged by size:
2114 1308 1208 46
BsiHKAI G_wGCw'C
Cuts at: 1690 2468 2658 3450 1690
Size: 778 190 792 2916
Fragments arranged by size:
2916 792 778 190
Bsp1286I G_dGCh'C
Cuts at: 1690 2301 2394 2468 2658 2720 3450 1690
Size: 611 93 74 190 62 730 2916
Fragments arranged by size:
2916 730 611 190 93 74 62
BspEI T'CCGG_A
Cuts at: 3896 3896
Size: 4676
BspHI T'CATG_A
Cuts at: 3852 3852
Size: 4676
BspMI ACCTGCnnnn'nnnn_
Cuts at: 1726 1940 2242 2623 1726
Size: 214 302 381 3779
Fragments arranged by size:
3779 381 302 214
BsrBI CCG'CTC
Cuts at: 50 2968 3022 4506 4671 50
Size: 2918 54 1484 165 55
Fragments arranged by size:
2918 1484 165 55 54
BsrDI GCAATG_nn'
Cuts at: 762 1292 2588 762
Size: 530 1296 2850
Fragments arranged by size:
2850 1296 530
BsrFI r'CCGG_y
Cuts at: 2674 2855 3064 2674
Size: 181 209 4286
Fragments arranged by size:
4286 209 181
BsrGI T'GTAC_A
Cuts at: 1524 1524
Size: 4676
BssSI C'ACGA_G
Cuts at: 2947 3305 2947
Size: 358 4318
BstAPI GCAn_nnn'nTGC
Cuts at: 2053 2053
Size: 4676
BstBI TT'CG_AA
Cuts at: 3037 3037
Size: 4676
BstYI r'GATC_y
Cuts at: 1 882 941 1106 1701 2168 2191 2526 2772
Size: 881 59 165 595 467 23 335 246
Cuts at: 2772 3784 3870 3882 4228 1
Size: 1012 86 12 346 449
Fragments arranged by size:
1012 881 595 467 449 346 335 246
165 86 59 23 12
BtgI C'CryG_G
Cuts at: 107 293 498 107
Size: 186 205 4285
Fragments arranged by size:
4285 205 186
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 2626 2807 2970 2626
Size: 181 163 4332
Fragments arranged by size:
4332 181 163
BtsI GCAGTG_nn'
Cuts at: 813 1134 1919 813
Size: 321 785 3570
Fragments arranged by size:
3570 785 321
DraI TTT'AAA
Cuts at: 1675 1675
Size: 4676
DraIII CAC_nnn'GTG
Cuts at: 1320 1320
Size: 4676
DrdI GACnn_nn'nnGTC
Cuts at: 2382 3240 2382
Size: 858 3818
EaeI y'GGCC_r
Cuts at: 431 2261 2435 2826 2853 4198 431
Size: 1830 174 391 27 1345 909
Fragments arranged by size:
1830 1345 909 391 174 27
EagI C'GGCC_G
Cuts at: 2261 2261
Size: 4676
EarI CTCTTCn'nnn_
Cuts at: 919 1136 2699 2909 919
Size: 217 1563 210 2686
Fragments arranged by size:
2686 1563 217 210
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 946 2884 3194 3340 4486 946
Size: 1938 310 146 1146 1136
Fragments arranged by size:
1938 1146 1136 310 146
EcoICRI GAG'CTC
Cuts at: 1688 1688
Size: 4676
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 169 2501 2933 3680 3948 169
Size: 2332 432 747 268 897
Fragments arranged by size:
2332 897 747 432 268
EcoO109I rG'GnC_Cy
Cuts at: 298 1815 298
Size: 1517 3159
EcoRI G'AATT_C
Cuts at: 1680 1680
Size: 4676
EcoRV GAT'ATC
Cuts at: 630 630
Size: 4676
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2695 2695
Size: 4676
FspI TGC'GCA
Cuts at: 2457 2457
Size: 4676
HaeII r_GCGC'y
Cuts at: 326 2110 2358 3380 4359 326
Size: 1784 248 1022 979 643
Fragments arranged by size:
1784 1022 979 643 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 867 899 949 981 1255 1287 2518 2548 2550
Size: 32 50 32 274 32 1231 30 2
Cuts at: 2550 2580 867
Size: 30 2963
Fragments arranged by size:
2963 1231 274 50 32 32 32 30
30 2
HincII GTy'rAC
Cuts at: 737 1715 737
Size: 978 3698
KasI G'GCGC_C
Cuts at: 2106 2354 2106
Size: 248 4428
KpnI G_GTAC'C
Cuts at: 1696 1696
Size: 4676
MluI A'CGCG_T
Cuts at: 4109 4109
Size: 4676
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1046 3347 3531 1046
Size: 2301 184 2191
Fragments arranged by size:
2301 2191 184
MscI TGG'CCA
Cuts at: 433 2437 433
Size: 2004 2672
MslI CAynn'nnrTG
Cuts at: 391 1168 1210 2665 2792 391
Size: 777 42 1455 127 2275
Fragments arranged by size:
2275 1455 777 127 42
MspA1I CmG'CkG
Cuts at: 306 732 1787 2101 2461 3474 3719 306
Size: 426 1055 314 360 1013 245 1263
Fragments arranged by size:
1263 1055 1013 426 360 314 245
NaeI GCC'GGC
Cuts at: 2857 2857
Size: 4676
NarI GG'CG_CC
Cuts at: 2107 2355 2107
Size: 248 4428
NcoI C'CATG_G
Cuts at: 107 107
Size: 4676
NgoMIV G'CCGG_C
Cuts at: 2855 2855
Size: 4676
NspI r_CATG'y
Cuts at: 441 586 981 1729 2229 3136 441
Size: 145 395 748 500 907 1981
Fragments arranged by size:
1981 907 748 500 395 145
PciI A'CATG_T
Cuts at: 3132 3132
Size: 4676
PflMI CCAn_nnn'nTGG
Cuts at: 863 1422 863
Size: 559 4117
PfoI T'CCnGG_A
Cuts at: 1358 1415 1358
Size: 57 4619
PmeI GTTT'AAAC
Cuts at: 1675 1675
Size: 4676
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3815 3847 3815
Size: 32 4644
PpuMI rG'GwC_Cy
Cuts at: 298 298
Size: 4676
PsiI TTA'TAA
Cuts at: 598 598
Size: 4676
PstI C_TGCA'G
Cuts at: 1043 1182 1377 1723 2408 1043
Size: 139 195 346 685 3311
Fragments arranged by size:
3311 685 346 195 139
PvuI CG_AT'CG
Cuts at: 104 104
Size: 4676
PvuII CAG'CTG
Cuts at: 2101 2461 2101
Size: 360 4316
RsrII CG'GwC_CG
Cuts at: 2871 2871
Size: 4676
SacI G_AGCT'C
Cuts at: 1690 1690
Size: 4676
SalI G'TCGA_C
Cuts at: 1713 1713
Size: 4676
SanDI GG'GwC_CC
Cuts at: 298 298
Size: 4676
SapI GCTCTTCn'nnn_
Cuts at: 2699 2909 2699
Size: 210 4466
SbfI CC_TGCA'GG
Cuts at: 1723 1723
Size: 4676
ScaI AGT'ACT
Cuts at: 1079 4193 1079
Size: 3114 1562
SfcI C'TryA_G
Cuts at: 33 176 522 615 705 1039 1178 1373 1719
Size: 143 346 93 90 334 139 195 346
Cuts at: 1719 2404 3397 3588 33
Size: 685 993 191 1121
Fragments arranged by size:
1121 993 685 346 346 334 195 191
143 139 93 90
SfoI GGC'GCC
Cuts at: 2108 2356 2108
Size: 248 4428
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 4676
SmaI CCC'GGG
Cuts at: 1698 1698
Size: 4676
SmlI C'TyrA_G
Cuts at: 820 1114 1831 2746 3238 3500 820
Size: 294 717 915 492 262 1996
Fragments arranged by size:
1996 915 717 492 294 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 4676
SphI G_CATG'C
Cuts at: 586 1729 2229 586
Size: 1143 500 3033
Fragments arranged by size:
3033 1143 500
SspI AAT'ATT
Cuts at: 1606 1606
Size: 4676
StyI C'CwwG_G
Cuts at: 107 1810 2164 107
Size: 1703 354 2619
Fragments arranged by size:
2619 1703 354
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 2259 2392 2851 3060 2259
Size: 133 459 209 3875
Fragments arranged by size:
3875 459 209 133
TatI w'GTAC_w
Cuts at: 1027 1077 1524 4191 1027
Size: 50 447 2667 1512
Fragments arranged by size:
2667 1512 447 50
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 4193 4225 4193
Size: 32 4644
Tth111I GACn'n_nGTC
Cuts at: 2473 2473
Size: 4676
XbaI T'CTAG_A
Cuts at: 1707 1707
Size: 4676
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2171 2171
Size: 4676
XmaI C'CCGG_G
Cuts at: 1696 1696
Size: 4676
XmnI GAAnn'nnTTC
Cuts at: 998 998
Size: 4676
Enzymes that do cut:
AccI Acc65I AclI AcuI AfeI AflIII AgeI AleI
AlfI AloI AlwNI ApaLI ApoI AseI AsiSI AvaI
BamHI BanI BanII BbeI BcgI BciVI BclI BglII
BlpI Bme1580I BmrI BplI BpmI BpuEI BsaI BsaAI
BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsiEI BsiHKAI
Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI
BssSI BstAPI BstBI BstYI BtgI BtgZI BtsI DraI
DraIII DrdI EaeI EagI EarI EciI EcoICRI Eco57MI
EcoO109I EcoRI EcoRV FalI FspI HaeII Hin4I HincII
KasI KpnI MluI MmeI MscI MslI MspA1I NaeI
NarI NcoI NgoMIV NspI PciI PflMI PfoI PmeI
PpiI PpuMI PsiI PstI PvuI PvuII RsrII SacI
SalI SanDI SapI SbfI ScaI SfcI SfoI SgfI
SmaI SmlI SpeI SphI SspI StyI TaqII TatI
TstI Tth111I XbaI XcmI XmaI XmnI
Enzymes that do not cut:
AarI AatII AflII AhdI ApaI AscI AvrII BaeI
BbsI BbvCI BfrBI BglI BmgBI BmtI Bpu10I BsgI
BsiWI BsmI BsmBI BssHII BstEII BstXI BstZ17I Bsu36I
ClaI CspCI EcoNI FseI FspAI HindIII HpaI MfeI
NdeI NheI NotI NruI NsiI PacI PasI PmlI
PshAI PspOMI PspXI PsrI SacII SexAI SfiI SgrAI
SnaBI SrfI StuI SwaI XhoI ZraI