Restriction map for
pF1KE0007
(Circular) (Six-base) MAPSORT of: pF1KE0007.seq Check: 8784 from: 1 to: 4667
pF1KE0007 4667 bp
With 182 enzymes: SgfI *
April 24, 2009 13:09 ..
AccI GT'mk_AC
Cuts at: 1705 1705
Size: 4667
Acc65I G'GTAC_C
Cuts at: 1683 1683
Size: 4667
AclI AA'CG_TT
Cuts at: 4507 4507
Size: 4667
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 2492 2924 3671 3939 2492
Size: 432 747 268 3220
Fragments arranged by size:
3220 747 432 268
AflIII A'CryG_T
Cuts at: 2019 3123 4100 2019
Size: 1104 977 2586
Fragments arranged by size:
2586 1104 977
AgeI A'CCGG_T
Cuts at: 3055 3055
Size: 4667
AhdI GACnn_n'nnGTC
Cuts at: 922 922
Size: 4667
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 447 1522 447
Size: 1075 3592
AlwNI CAG_nnn'CTG
Cuts at: 1972 3539 1972
Size: 1567 3100
ApaLI G'TGCA_C
Cuts at: 3437 3437
Size: 4667
ApoI r'AATT_y
Cuts at: 253 582 996 1671 4020 4276 253
Size: 329 414 675 2349 256 644
Fragments arranged by size:
2349 675 644 414 329 256
AseI AT'TA_AT
Cuts at: 20 20
Size: 4667
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 4667
AvaI C'yCGr_G
Cuts at: 1687 1687
Size: 4667
BamHI G'GATC_C
Cuts at: 1692 1692
Size: 4667
BanI G'GyrC_C
Cuts at: 1582 1683 2097 2345 2380 1582
Size: 101 414 248 35 3869
Fragments arranged by size:
3869 414 248 101 35
BanII G_rGCy'C
Cuts at: 596 1681 2711 596
Size: 1085 1030 2552
Fragments arranged by size:
2552 1085 1030
BbeI G_GCGC'C
Cuts at: 2101 2349 2101
Size: 248 4419
BbsI GAAGACnn'nnnn_
Cuts at: 780 780
Size: 4667
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 90 124 90
Size: 34 4633
BciVI GTATCCnnnnn_n'
Cuts at: 144 2565 3326 4667 144
Size: 2421 761 1341 144
Fragments arranged by size:
2421 1341 761 144
BclI T'GATC_A
Cuts at: 2187 2187
Size: 4667
BglII A'GATC_T
Cuts at: 2182 4219 2182
Size: 2037 2630
BlpI GC'TnA_GC
Cuts at: 1779 1779
Size: 4667
Bme1580I G_kGCm'C
Cuts at: 2292 2385 3441 2292
Size: 93 1056 3518
Fragments arranged by size:
3518 1056 93
BmrI ACTGGGnnnn_n'
Cuts at: 898 1879 1960 2295 4439 898
Size: 981 81 335 2144 1126
Fragments arranged by size:
2144 1126 981 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3034 3034
Size: 4667
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 580 580
Size: 4667
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 739 879 1549 1843 2722 3214 3512 739
Size: 140 670 294 879 492 298 1894
Fragments arranged by size:
1894 879 670 492 298 294 140
BsaI GGTCTCn'nnnn_
Cuts at: 4371 4371
Size: 4667
BsaAI yAC'GTr
Cuts at: 2020 2020
Size: 4667
BsaBI GATnn'nnATC
Cuts at: 6 2181 4218 6
Size: 2175 2037 455
Fragments arranged by size:
2175 2037 455
BsaHI Gr'CG_yC
Cuts at: 2098 2346 3048 4544 2098
Size: 248 702 1496 2221
Fragments arranged by size:
2221 1496 702 248
BsaWI w'CCGG_w
Cuts at: 1856 2377 3055 3329 3476 3887 1856
Size: 521 678 274 147 411 2636
Fragments arranged by size:
2636 678 521 411 274 147
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 604 604
Size: 4667
BseYI C'CCAG_C
Cuts at: 1333 1556 2092 2850 3427 3923 1333
Size: 223 536 758 577 496 2077
Fragments arranged by size:
2077 758 577 536 496 223
BsiEI CG_ry'CG
Cuts at: 104 2209 2255 3463 104
Size: 2105 46 1208 1308
Fragments arranged by size:
2105 1308 1208 46
BsiHKAI G_wGCw'C
Cuts at: 230 596 604 1681 2459 2649 3441 230
Size: 366 8 1077 778 190 792 1456
Fragments arranged by size:
1456 1077 792 778 366 190 8
BsmI GAATG_Cn'
Cuts at: 622 1115 1501 1534 622
Size: 493 386 33 3755
Fragments arranged by size:
3755 493 386 33
BsmBI CGTCTCn'nnnn_
Cuts at: 634 1507 634
Size: 873 3794
Bsp1286I G_dGCh'C
Cuts at: 230 596 604 1681 2292 2385 2459 2649 2711
Size: 366 8 1077 611 93 74 190 62
Cuts at: 2711 3441 230
Size: 730 1456
Fragments arranged by size:
1456 1077 730 611 366 190 93 74
62 8
BspEI T'CCGG_A
Cuts at: 3887 3887
Size: 4667
BspHI T'CATG_A
Cuts at: 1151 3843 1151
Size: 2692 1975
BspMI ACCTGCnnnn'nnnn_
Cuts at: 373 1469 1717 1931 2233 2614 373
Size: 1096 248 214 302 381 2426
Fragments arranged by size:
2426 1096 381 302 248 214
BsrBI CCG'CTC
Cuts at: 50 1438 2959 3013 4497 4662 50
Size: 1388 1521 54 1484 165 55
Fragments arranged by size:
1521 1484 1388 165 55 54
BsrDI GCAATG_nn'
Cuts at: 2579 2579
Size: 4667
BsrFI r'CCGG_y
Cuts at: 1579 2665 2846 3055 1579
Size: 1086 181 209 3191
Fragments arranged by size:
3191 1086 209 181
BsrGI T'GTAC_A
Cuts at: 696 780 1353 696
Size: 84 573 4010
Fragments arranged by size:
4010 573 84
BssSI C'ACGA_G
Cuts at: 306 2938 3296 306
Size: 2632 358 1677
Fragments arranged by size:
2632 1677 358
BstAPI GCAn_nnn'nTGC
Cuts at: 1110 2044 1110
Size: 934 3733
BstBI TT'CG_AA
Cuts at: 3028 3028
Size: 4667
BstXI CCAn_nnnn'nTGG
Cuts at: 921 921
Size: 4667
BstYI r'GATC_y
Cuts at: 1 1692 2159 2182 2517 2763 3775 3861 3873
Size: 1691 467 23 335 246 1012 86 12
Cuts at: 3873 4219 1
Size: 346 449
Fragments arranged by size:
1691 1012 467 449 346 335 246 86
23 12
BtgI C'CryG_G
Cuts at: 204 1047 1189 204
Size: 843 142 3682
Fragments arranged by size:
3682 843 142
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 2617 2798 2961 2617
Size: 181 163 4323
Fragments arranged by size:
4323 181 163
BtsI GCAGTG_nn'
Cuts at: 481 1501 1910 481
Size: 1020 409 3238
Fragments arranged by size:
3238 1020 409
DraI TTT'AAA
Cuts at: 641 1666 641
Size: 1025 3642
DrdI GACnn_nn'nnGTC
Cuts at: 2373 3231 2373
Size: 858 3809
EaeI y'GGCC_r
Cuts at: 233 762 1571 2252 2426 2817 2844 4189 233
Size: 529 809 681 174 391 27 1345 711
Fragments arranged by size:
1345 809 711 681 529 391 174 27
EagI C'GGCC_G
Cuts at: 2252 2252
Size: 4667
EarI CTCTTCn'nnn_
Cuts at: 2690 2900 2690
Size: 210 4457
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 2875 3185 3331 4477 2875
Size: 310 146 1146 3065
Fragments arranged by size:
3065 1146 310 146
EcoICRI GAG'CTC
Cuts at: 594 1679 594
Size: 1085 3582
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 580 2492 2924 3671 3939 580
Size: 1912 432 747 268 1308
Fragments arranged by size:
1912 1308 747 432 268
EcoO109I rG'GnC_Cy
Cuts at: 1093 1216 1806 1093
Size: 123 590 3954
Fragments arranged by size:
3954 590 123
EcoRI G'AATT_C
Cuts at: 1671 1671
Size: 4667
EcoRV GAT'ATC
Cuts at: 891 891
Size: 4667
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2686 2686
Size: 4667
FspI TGC'GCA
Cuts at: 2448 2448
Size: 4667
HaeII r_GCGC'y
Cuts at: 2101 2349 3371 4350 2101
Size: 248 1022 979 2418
Fragments arranged by size:
2418 1022 979 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 908 940 2509 2539 2541 2571 908
Size: 32 1569 30 2 30 3004
Fragments arranged by size:
3004 1569 32 30 30 2
HincII GTy'rAC
Cuts at: 1706 1706
Size: 4667
HindIII A'AGCT_T
Cuts at: 247 247
Size: 4667
KasI G'GCGC_C
Cuts at: 2097 2345 2097
Size: 248 4419
KpnI G_GTAC'C
Cuts at: 1687 1687
Size: 4667
MluI A'CGCG_T
Cuts at: 4100 4100
Size: 4667
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 3338 3522 3338
Size: 184 4483
MscI TGG'CCA
Cuts at: 235 764 1573 2428 235
Size: 529 809 855 2474
Fragments arranged by size:
2474 855 809 529
MslI CAynn'nnrTG
Cuts at: 574 1526 2656 2783 574
Size: 952 1130 127 2458
Fragments arranged by size:
2458 1130 952 127
MspA1I CmG'CkG
Cuts at: 1337 1542 1778 2092 2452 3465 3710 1337
Size: 205 236 314 360 1013 245 2294
Fragments arranged by size:
2294 1013 360 314 245 236 205
NaeI GCC'GGC
Cuts at: 2848 2848
Size: 4667
NarI GG'CG_CC
Cuts at: 2098 2346 2098
Size: 248 4419
NcoI C'CATG_G
Cuts at: 204 1047 1189 204
Size: 843 142 3682
Fragments arranged by size:
3682 843 142
NgoMIV G'CCGG_C
Cuts at: 2846 2846
Size: 4667
NspI r_CATG'y
Cuts at: 1485 1720 2220 3127 1485
Size: 235 500 907 3025
Fragments arranged by size:
3025 907 500 235
PciI A'CATG_T
Cuts at: 3123 3123
Size: 4667
PfoI T'CCnGG_A
Cuts at: 1454 1454
Size: 4667
PmeI GTTT'AAAC
Cuts at: 1666 1666
Size: 4667
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3806 3838 3806
Size: 32 4635
PpuMI rG'GwC_Cy
Cuts at: 1093 1216 1093
Size: 123 4544
PshAI GACnn'nnGTC
Cuts at: 156 458 156
Size: 302 4365
PsiI TTA'TAA
Cuts at: 317 317
Size: 4667
PsrI GAACnnnnnnTACnnnnnnn_nnnnn'
Cuts at: 1067 1099 1067
Size: 32 4635
PstI C_TGCA'G
Cuts at: 1283 1346 1714 2399 1283
Size: 63 368 685 3551
Fragments arranged by size:
3551 685 368 63
PvuI CG_AT'CG
Cuts at: 104 104
Size: 4667
PvuII CAG'CTG
Cuts at: 1542 2092 2452 1542
Size: 550 360 3757
Fragments arranged by size:
3757 550 360
RsrII CG'GwC_CG
Cuts at: 2862 2862
Size: 4667
SacI G_AGCT'C
Cuts at: 596 1681 596
Size: 1085 3582
SalI G'TCGA_C
Cuts at: 1704 1704
Size: 4667
SapI GCTCTTCn'nnn_
Cuts at: 2690 2900 2690
Size: 210 4457
SbfI CC_TGCA'GG
Cuts at: 1714 1714
Size: 4667
ScaI AGT'ACT
Cuts at: 4184 4184
Size: 4667
SfcI C'TryA_G
Cuts at: 33 325 932 1279 1342 1710 2395 3388 3579
Size: 292 607 347 63 368 685 993 191
Cuts at: 3579 33
Size: 1121
Fragments arranged by size:
1121 993 685 607 368 347 292 191
63
SfoI GGC'GCC
Cuts at: 2099 2347 2099
Size: 248 4419
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 4667
SmaI CCC'GGG
Cuts at: 1689 1689
Size: 4667
SmlI C'TyrA_G
Cuts at: 754 894 1564 1822 2737 3229 3491 754
Size: 140 670 258 915 492 262 1930
Fragments arranged by size:
1930 915 670 492 262 258 140
SpeI A'CTAG_T
Cuts at: 67 67
Size: 4667
SphI G_CATG'C
Cuts at: 1720 2220 1720
Size: 500 4167
StyI C'CwwG_G
Cuts at: 204 611 1047 1189 1801 2155 204
Size: 407 436 142 612 354 2716
Fragments arranged by size:
2716 612 436 407 354 142
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 2250 2383 2842 3051 2250
Size: 133 459 209 3866
Fragments arranged by size:
3866 459 209 133
TatI w'GTAC_w
Cuts at: 696 780 1353 4182 696
Size: 84 573 2829 1181
Fragments arranged by size:
2829 1181 573 84
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 4184 4216 4184
Size: 32 4635
Tth111I GACn'n_nGTC
Cuts at: 2464 2464
Size: 4667
XbaI T'CTAG_A
Cuts at: 1698 1698
Size: 4667
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 114 2162 114
Size: 2048 2619
XmaI C'CCGG_G
Cuts at: 1687 1687
Size: 4667
Enzymes that do cut:
AccI Acc65I AclI AcuI AflIII AgeI AhdI AlfI
AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI BanI
BanII BbeI BbsI BcgI BciVI BclI BglII BlpI
Bme1580I BmrI BplI BpmI BpuEI BsaI BsaAI BsaBI
BsaHI BsaWI BseRI BseYI BsiEI BsiHKAI BsmI BsmBI
Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI
BssSI BstAPI BstBI BstXI BstYI BtgI BtgZI BtsI
DraI DrdI EaeI EagI EarI EciI EcoICRI Eco57MI
EcoO109I EcoRI EcoRV FalI FspI HaeII Hin4I HincII
HindIII KasI KpnI MluI MmeI MscI MslI MspA1I
NaeI NarI NcoI NgoMIV NspI PciI PfoI PmeI
PpiI PpuMI PshAI PsiI PsrI PstI PvuI PvuII
RsrII SacI SalI SapI SbfI ScaI SfcI SfoI
SgfI SmaI SmlI SpeI SphI StyI TaqII TatI
TstI Tth111I XbaI XcmI XmaI
Enzymes that do not cut:
AarI AatII AfeI AflII AleI AloI ApaI AscI
AvrII BaeI BbvCI BfrBI BglI BmgBI BmtI Bpu10I
BsaXI BsgI BsiWI BssHII BstEII BstZ17I Bsu36I ClaI
CspCI DraIII EcoNI FseI FspAI HpaI MfeI NdeI
NheI NotI NruI NsiI PacI PasI PflMI PmlI
PspOMI PspXI SacII SanDI SexAI SfiI SgrAI SnaBI
SrfI SspI StuI SwaI XhoI XmnI ZraI