Restriction map for
pF1KE0010
(Circular) (Six-base) MAPSORT of: pF1KE0010.seq Check: 8286 from: 1 to: 5423
pF1KE0010 5423 bp
With 182 enzymes: SgfI *
April 24, 2009 13:09 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 1133 1133
Size: 5423
AccI GT'mk_AC
Cuts at: 1194 2461 1194
Size: 1267 4156
Acc65I G'GTAC_C
Cuts at: 2439 2439
Size: 5423
AclI AA'CG_TT
Cuts at: 5263 5263
Size: 5423
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 515 1466 3248 3680 4427 4695 515
Size: 951 1782 432 747 268 1243
Fragments arranged by size:
1782 1243 951 747 432 268
AflII C'TTAA_G
Cuts at: 1812 1812
Size: 5423
AflIII A'CryG_T
Cuts at: 2775 3879 4856 2775
Size: 1104 977 3342
Fragments arranged by size:
3342 1104 977
AgeI A'CCGG_T
Cuts at: 3811 3811
Size: 5423
AleI CACnn'nnGTG
Cuts at: 2140 2140
Size: 5423
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 482 514 2245 2277 482
Size: 32 1731 32 3628
Fragments arranged by size:
3628 1731 32 32
AlwNI CAG_nnn'CTG
Cuts at: 171 482 550 732 1444 2218 2373 2728 4295
Size: 311 68 182 712 774 155 355 1567
Cuts at: 4295 171
Size: 1299
Fragments arranged by size:
1567 1299 774 712 355 311 182 155
68
ApaLI G'TGCA_C
Cuts at: 4193 4193
Size: 5423
ApoI r'AATT_y
Cuts at: 471 1790 2427 4776 5032 471
Size: 1319 637 2349 256 862
Fragments arranged by size:
2349 1319 862 637 256
AseI AT'TA_AT
Cuts at: 20 20
Size: 5423
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 5423
AvaI C'yCGr_G
Cuts at: 2443 2443
Size: 5423
BamHI G'GATC_C
Cuts at: 1302 2448 1302
Size: 1146 4277
BanI G'GyrC_C
Cuts at: 685 2439 2853 3101 3136 685
Size: 1754 414 248 35 2972
Fragments arranged by size:
2972 1754 414 248 35
BanII G_rGCy'C
Cuts at: 1549 2437 3467 1549
Size: 888 1030 3505
Fragments arranged by size:
3505 1030 888
BbeI G_GCGC'C
Cuts at: 2857 3105 2857
Size: 248 5175
BbsI GAAGACnn'nnnn_
Cuts at: 2204 2204
Size: 5423
BciVI GTATCCnnnnn_n'
Cuts at: 1885 2405 3321 4082 5423 1885
Size: 520 916 761 1341 1885
Fragments arranged by size:
1885 1341 916 761 520
BclI T'GATC_A
Cuts at: 195 2943 195
Size: 2748 2675
BfrBI ATG'CAT
Cuts at: 160 160
Size: 5423
BglI GCCn_nnn'nGGC
Cuts at: 576 576
Size: 5423
BglII A'GATC_T
Cuts at: 1138 2938 4975 1138
Size: 1800 2037 1586
Fragments arranged by size:
2037 1800 1586
BlpI GC'TnA_GC
Cuts at: 2535 2535
Size: 5423
Bme1580I G_kGCm'C
Cuts at: 3048 3141 4197 3048
Size: 93 1056 4274
Fragments arranged by size:
4274 1056 93
BmrI ACTGGGnnnn_n'
Cuts at: 181 2635 2716 3051 5195 181
Size: 2454 81 335 2144 409
Fragments arranged by size:
2454 2144 409 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 821 3790 821
Size: 2969 2454
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 874 1447 1534 2131 2404 874
Size: 573 87 597 273 3893
Fragments arranged by size:
3893 597 573 273 87
Bpu10I CC'TnA_GC
Cuts at: 530 2185 530
Size: 1655 3768
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2599 3478 3970 4268 2599
Size: 879 492 298 3754
Fragments arranged by size:
3754 879 492 298
BsaI GGTCTCn'nnnn_
Cuts at: 1350 5127 1350
Size: 3777 1646
BsaAI yAC'GTr
Cuts at: 2776 2776
Size: 5423
BsaBI GATnn'nnATC
Cuts at: 6 2937 4974 6
Size: 2931 2037 455
Fragments arranged by size:
2931 2037 455
BsaHI Gr'CG_yC
Cuts at: 2854 3102 3804 5300 2854
Size: 248 702 1496 2977
Fragments arranged by size:
2977 1496 702 248
BsaWI w'CCGG_w
Cuts at: 1161 1271 2612 3133 3811 4085 4232 4643 1161
Size: 110 1341 521 678 274 147 411 1941
Fragments arranged by size:
1941 1341 678 521 411 274 147 110
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1074 1104 1420 1450 1721 1751 2245 2275 1074
Size: 30 316 30 271 30 494 30 4222
Fragments arranged by size:
4222 494 316 271 30 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 370 926 1677 2164 370
Size: 556 751 487 3629
Fragments arranged by size:
3629 751 556 487
BseYI C'CCAG_C
Cuts at: 712 943 1038 2848 3606 4183 4679 712
Size: 231 95 1810 758 577 496 1456
Fragments arranged by size:
1810 1456 758 577 496 231 95
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 248 451 2123 248
Size: 203 1672 3548
Fragments arranged by size:
3548 1672 203
BsiEI CG_ry'CG
Cuts at: 104 2965 3011 4219 104
Size: 2861 46 1208 1308
Fragments arranged by size:
2861 1308 1208 46
BsiHKAI G_wGCw'C
Cuts at: 900 918 1071 1520 2147 2437 3215 3405 4197
Size: 18 153 449 627 290 778 190 792
Cuts at: 4197 900
Size: 2126
Fragments arranged by size:
2126 792 778 627 449 290 190 153
18
Bsp1286I G_dGCh'C
Cuts at: 900 918 1071 1520 1549 2147 2437 3048 3141
Size: 18 153 449 29 598 290 611 93
Cuts at: 3141 3215 3405 3467 4197 900
Size: 74 190 62 730 2126
Fragments arranged by size:
2126 730 611 598 449 290 190 153
93 74 62 29 18
BspEI T'CCGG_A
Cuts at: 4643 4643
Size: 5423
BspHI T'CATG_A
Cuts at: 1946 4599 1946
Size: 2653 2770
BspMI ACCTGCnnnn'nnnn_
Cuts at: 1133 1770 2473 2687 2989 3370 1133
Size: 637 703 214 302 381 3186
Fragments arranged by size:
3186 703 637 381 302 214
BsrBI CCG'CTC
Cuts at: 50 3715 3769 5253 5418 50
Size: 3665 54 1484 165 55
Fragments arranged by size:
3665 1484 165 55 54
BsrDI GCAATG_nn'
Cuts at: 321 3335 321
Size: 3014 2409
BsrFI r'CCGG_y
Cuts at: 2363 3421 3602 3811 2363
Size: 1058 181 209 3975
Fragments arranged by size:
3975 1058 209 181
BsrGI T'GTAC_A
Cuts at: 1860 1860
Size: 5423
BssSI C'ACGA_G
Cuts at: 2273 3694 4052 2273
Size: 1421 358 3644
Fragments arranged by size:
3644 1421 358
BstAPI GCAn_nnn'nTGC
Cuts at: 2800 2800
Size: 5423
BstBI TT'CG_AA
Cuts at: 3784 3784
Size: 5423
BstEII G'GTnAC_C
Cuts at: 346 847 346
Size: 501 4922
BstYI r'GATC_y
Cuts at: 1 408 490 1138 1302 2448 2915 2938 3273
Size: 407 82 648 164 1146 467 23 335
Cuts at: 3273 3519 4531 4617 4629 4975 1
Size: 246 1012 86 12 346 449
Fragments arranged by size:
1146 1012 648 467 449 407 346 335
246 164 86 82 23 12
Bsu36I CC'TnA_GG
Cuts at: 1499 1499
Size: 5423
BtgI C'CryG_G
Cuts at: 2190 2190
Size: 5423
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 3373 3554 3717 3373
Size: 181 163 5079
Fragments arranged by size:
5079 181 163
BtsI GCAGTG_nn'
Cuts at: 434 520 828 2135 2167 2666 434
Size: 86 308 1307 32 499 3191
Fragments arranged by size:
3191 1307 499 308 86 32
DraI TTT'AAA
Cuts at: 2422 2422
Size: 5423
DrdI GACnn_nn'nnGTC
Cuts at: 3129 3987 3129
Size: 858 4565
EaeI y'GGCC_r
Cuts at: 1505 2306 3008 3182 3573 3600 4945 1505
Size: 801 702 174 391 27 1345 1983
Fragments arranged by size:
1983 1345 801 702 391 174 27
EagI C'GGCC_G
Cuts at: 3008 3008
Size: 5423
EarI CTCTTCn'nnn_
Cuts at: 182 840 1748 1882 3446 3656 182
Size: 658 908 134 1564 210 1949
Fragments arranged by size:
1949 1564 908 658 210 134
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 3631 3941 4087 5233 3631
Size: 310 146 1146 3821
Fragments arranged by size:
3821 1146 310 146
EcoICRI GAG'CTC
Cuts at: 2435 2435
Size: 5423
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 515 874 1447 1466 1534 2131 2404 3248 3680
Size: 359 573 19 68 597 273 844 432
Cuts at: 3680 4427 4695 515
Size: 747 268 1243
Fragments arranged by size:
1243 844 747 597 573 432 359 273
268 68 19
EcoNI CCTnn'n_nnAGG
Cuts at: 1112 1112
Size: 5423
EcoO109I rG'GnC_Cy
Cuts at: 578 606 960 1117 1146 2562 578
Size: 28 354 157 29 1416 3439
Fragments arranged by size:
3439 1416 354 157 29 28
EcoRI G'AATT_C
Cuts at: 471 2427 471
Size: 1956 3467
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 3442 3442
Size: 5423
FspI TGC'GCA
Cuts at: 3204 3204
Size: 5423
HaeII r_GCGC'y
Cuts at: 264 2857 3105 4127 5106 264
Size: 2593 248 1022 979 581
Fragments arranged by size:
2593 1022 979 581 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 720 752 787 819 1266 1298 1330 1362 1420
Size: 32 35 32 447 32 32 32 58
Cuts at: 1420 1452 1721 1753 3265 3295 3297 3327 720
Size: 32 269 32 1512 30 2 30 2816
Fragments arranged by size:
2816 1512 447 269 58 35 32 32
32 32 32 32 32 30 30 2
HincII GTy'rAC
Cuts at: 638 1195 2361 2462 638
Size: 557 1166 101 3599
Fragments arranged by size:
3599 1166 557 101
HindIII A'AGCT_T
Cuts at: 1486 1558 1486
Size: 72 5351
KasI G'GCGC_C
Cuts at: 2853 3101 2853
Size: 248 5175
KpnI G_GTAC'C
Cuts at: 2443 2443
Size: 5423
MluI A'CGCG_T
Cuts at: 4856 4856
Size: 5423
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 4094 4278 4094
Size: 184 5239
MscI TGG'CCA
Cuts at: 1507 2308 3184 1507
Size: 801 876 3746
Fragments arranged by size:
3746 876 801
MslI CAynn'nnrTG
Cuts at: 1945 2140 3412 3539 1945
Size: 195 1272 127 3829
Fragments arranged by size:
3829 1272 195 127
MspA1I CmG'CkG
Cuts at: 1329 1441 1936 2534 2848 3208 4221 4466 1329
Size: 112 495 598 314 360 1013 245 2286
Fragments arranged by size:
2286 1013 598 495 360 314 245 112
NaeI GCC'GGC
Cuts at: 3604 3604
Size: 5423
NarI GG'CG_CC
Cuts at: 2854 3102 2854
Size: 248 5175
NdeI CA'TA_TG
Cuts at: 1308 1308
Size: 5423
NgoMIV G'CCGG_C
Cuts at: 3602 3602
Size: 5423
NsiI A_TGCA'T
Cuts at: 162 162
Size: 5423
NspI r_CATG'y
Cuts at: 1803 2476 2976 3883 1803
Size: 673 500 907 3343
Fragments arranged by size:
3343 907 673 500
PasI CC'CwG_GG
Cuts at: 585 585
Size: 5423
PciI A'CATG_T
Cuts at: 3879 3879
Size: 5423
PflMI CCAn_nnn'nTGG
Cuts at: 482 482
Size: 5423
PmeI GTTT'AAAC
Cuts at: 2422 2422
Size: 5423
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2245 2277 4562 4594 2245
Size: 32 2285 32 3074
Fragments arranged by size:
3074 2285 32 32
PpuMI rG'GwC_Cy
Cuts at: 606 960 1117 606
Size: 354 157 4912
Fragments arranged by size:
4912 354 157
PshAI GACnn'nnGTC
Cuts at: 936 936
Size: 5423
PsiI TTA'TAA
Cuts at: 1681 1681
Size: 5423
PsrI GAACnnnnnnTACnnnnnnn_nnnnn'
Cuts at: 608 640 608
Size: 32 5391
PstI C_TGCA'G
Cuts at: 823 2399 2470 3155 823
Size: 1576 71 685 3091
Fragments arranged by size:
3091 1576 685 71
PvuI CG_AT'CG
Cuts at: 104 104
Size: 5423
PvuII CAG'CTG
Cuts at: 1441 1936 2848 3208 1441
Size: 495 912 360 3656
Fragments arranged by size:
3656 912 495 360
RsrII CG'GwC_CG
Cuts at: 3618 3618
Size: 5423
SacI G_AGCT'C
Cuts at: 2437 2437
Size: 5423
SalI G'TCGA_C
Cuts at: 1193 2460 1193
Size: 1267 4156
SanDI GG'GwC_CC
Cuts at: 960 960
Size: 5423
SapI GCTCTTCn'nnn_
Cuts at: 1748 3446 3656 1748
Size: 1698 210 3515
Fragments arranged by size:
3515 1698 210
SbfI CC_TGCA'GG
Cuts at: 2470 2470
Size: 5423
ScaI AGT'ACT
Cuts at: 4940 4940
Size: 5423
SfcI C'TryA_G
Cuts at: 33 542 819 922 1695 2203 2222 2395 2466
Size: 509 277 103 773 508 19 173 71
Cuts at: 2466 3151 4144 4335 33
Size: 685 993 191 1121
Fragments arranged by size:
1121 993 773 685 509 508 277 191
173 103 71 19
SfoI GGC'GCC
Cuts at: 2855 3103 2855
Size: 248 5175
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 5423
SmaI CCC'GGG
Cuts at: 2445 2445
Size: 5423
SmlI C'TyrA_G
Cuts at: 1812 2578 3493 3985 4247 1812
Size: 766 915 492 262 2988
Fragments arranged by size:
2988 915 766 492 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 5423
SphI G_CATG'C
Cuts at: 2476 2976 2476
Size: 500 4923
StuI AGG'CCT
Cuts at: 1842 1842
Size: 5423
StyI C'CwwG_G
Cuts at: 628 2557 2911 628
Size: 1929 354 3140
Fragments arranged by size:
3140 1929 354
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 334 736 3006 3139 3598 3807 334
Size: 402 2270 133 459 209 1950
Fragments arranged by size:
2270 1950 459 402 209 133
TatI w'GTAC_w
Cuts at: 1860 4938 1860
Size: 3078 2345
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 1034 1066 4940 4972 1034
Size: 32 3874 32 1485
Fragments arranged by size:
3874 1485 32 32
Tth111I GACn'n_nGTC
Cuts at: 3220 3220
Size: 5423
XbaI T'CTAG_A
Cuts at: 2454 2454
Size: 5423
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2155 2918 2155
Size: 763 4660
XmaI C'CCGG_G
Cuts at: 2443 2443
Size: 5423
Enzymes that do cut:
AarI AccI Acc65I AclI AcuI AflII AflIII AgeI
AleI AloI AlwNI ApaLI ApoI AseI AsiSI AvaI
BamHI BanI BanII BbeI BbsI BciVI BclI BfrBI
BglI BglII BlpI Bme1580I BmrI BplI BpmI Bpu10I
BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI
BseYI BsgI BsiEI BsiHKAI Bsp1286I BspEI BspHI BspMI
BsrBI BsrDI BsrFI BsrGI BssSI BstAPI BstBI BstEII
BstYI Bsu36I BtgI BtgZI BtsI DraI DrdI EaeI
EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI
FalI FspI HaeII Hin4I HincII HindIII KasI KpnI
MluI MmeI MscI MslI MspA1I NaeI NarI NdeI
NgoMIV NsiI NspI PasI PciI PflMI PmeI PpiI
PpuMI PshAI PsiI PsrI PstI PvuI PvuII RsrII
SacI SalI SanDI SapI SbfI ScaI SfcI SfoI
SgfI SmaI SmlI SpeI SphI StuI StyI TaqII
TatI TstI Tth111I XbaI XcmI XmaI
Enzymes that do not cut:
AatII AfeI AhdI AlfI ApaI AscI AvrII BaeI
BbvCI BcgI BmgBI BmtI BsiWI BsmI BsmBI BssHII
BstXI BstZ17I ClaI CspCI DraIII EcoRV FseI FspAI
HpaI MfeI NcoI NheI NotI NruI PacI PfoI
PmlI PspOMI PspXI SacII SexAI SfiI SgrAI SnaBI
SrfI SspI SwaI XhoI XmnI ZraI