Restriction map for
pF1KE0012
(Circular) (Six-base) MAPSORT of: pF1KE0012.seq Check: 8082 from: 1 to: 4676
pF1KE0012 4676 bp
With 182 enzymes: SgfI *
April 24, 2009 13:09 ..
AccI GT'mk_AC
Cuts at: 1714 1714
Size: 4676
Acc65I G'GTAC_C
Cuts at: 183 1692 183
Size: 1509 3167
AclI AA'CG_TT
Cuts at: 4516 4516
Size: 4676
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 302 904 1192 2501 2933 3680 3948 302
Size: 602 288 1309 432 747 268 1030
Fragments arranged by size:
1309 1030 747 602 432 288 268
AflII C'TTAA_G
Cuts at: 292 292
Size: 4676
AflIII A'CryG_T
Cuts at: 1035 2028 3132 4109 1035
Size: 993 1104 977 1602
Fragments arranged by size:
1602 1104 993 977
AgeI A'CCGG_T
Cuts at: 3064 3064
Size: 4676
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 106 138 106
Size: 32 4644
AlwNI CAG_nnn'CTG
Cuts at: 1981 3548 1981
Size: 1567 3109
ApaLI G'TGCA_C
Cuts at: 3446 3446
Size: 4676
ApoI r'AATT_y
Cuts at: 1465 1537 1551 1680 4029 4285 1465
Size: 72 14 129 2349 256 1856
Fragments arranged by size:
2349 1856 256 129 72 14
AseI AT'TA_AT
Cuts at: 20 20
Size: 4676
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 4676
AvaI C'yCGr_G
Cuts at: 1696 1696
Size: 4676
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'
Cuts at: 1076 1109 1076
Size: 33 4643
BamHI G'GATC_C
Cuts at: 1701 1701
Size: 4676
BanI G'GyrC_C
Cuts at: 183 1692 2106 2354 2389 183
Size: 1509 414 248 35 2470
Fragments arranged by size:
2470 1509 414 248 35
BanII G_rGCy'C
Cuts at: 1690 2720 1690
Size: 1030 3646
BbeI G_GCGC'C
Cuts at: 2110 2358 2110
Size: 248 4428
BbsI GAAGACnn'nnnn_
Cuts at: 1198 1198
Size: 4676
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 90 124 90
Size: 34 4642
BciVI GTATCCnnnnn_n'
Cuts at: 2574 3335 4676 2574
Size: 761 1341 2574
Fragments arranged by size:
2574 1341 761
BclI T'GATC_A
Cuts at: 432 699 2196 432
Size: 267 1497 2912
Fragments arranged by size:
2912 1497 267
BfrBI ATG'CAT
Cuts at: 329 329
Size: 4676
BglI GCCn_nnn'nGGC
Cuts at: 485 1029 485
Size: 544 4132
BglII A'GATC_T
Cuts at: 1281 2191 4228 1281
Size: 910 2037 1729
Fragments arranged by size:
2037 1729 910
BlpI GC'TnA_GC
Cuts at: 769 1788 769
Size: 1019 3657
Bme1580I G_kGCm'C
Cuts at: 1025 2301 2394 3450 1025
Size: 1276 93 1056 2251
Fragments arranged by size:
2251 1276 1056 93
BmrI ACTGGGnnnn_n'
Cuts at: 120 1352 1888 1969 2304 4448 120
Size: 1232 536 81 335 2144 348
Fragments arranged by size:
2144 1232 536 348 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3043 3043
Size: 4676
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1030 1852 2731 3223 3521 1030
Size: 822 879 492 298 2185
Fragments arranged by size:
2185 879 822 492 298
BsaI GGTCTCn'nnnn_
Cuts at: 4380 4380
Size: 4676
BsaAI yAC'GTr
Cuts at: 2029 2029
Size: 4676
BsaBI GATnn'nnATC
Cuts at: 6 1165 2190 4227 6
Size: 1159 1025 2037 455
Fragments arranged by size:
2037 1159 1025 455
BsaHI Gr'CG_yC
Cuts at: 2107 2355 3057 4553 2107
Size: 248 702 1496 2230
Fragments arranged by size:
2230 1496 702 248
BsaWI w'CCGG_w
Cuts at: 1865 2386 3064 3338 3485 3896 1865
Size: 521 678 274 147 411 2645
Fragments arranged by size:
2645 678 521 411 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1208 1238 1208
Size: 30 4646
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 544 544
Size: 4676
BseYI C'CCAG_C
Cuts at: 2101 2859 3436 3932 2101
Size: 758 577 496 2845
Fragments arranged by size:
2845 758 577 496
BsiEI CG_ry'CG
Cuts at: 104 2218 2264 3472 104
Size: 2114 46 1208 1308
Fragments arranged by size:
2114 1308 1208 46
BsiHKAI G_wGCw'C
Cuts at: 1690 2468 2658 3450 1690
Size: 778 190 792 2916
Fragments arranged by size:
2916 792 778 190
BsmI GAATG_Cn'
Cuts at: 481 481
Size: 4676
Bsp1286I G_dGCh'C
Cuts at: 1025 1690 2301 2394 2468 2658 2720 3450 1025
Size: 665 611 93 74 190 62 730 2251
Fragments arranged by size:
2251 730 665 611 190 93 74 62
BspEI T'CCGG_A
Cuts at: 3896 3896
Size: 4676
BspHI T'CATG_A
Cuts at: 969 3852 969
Size: 2883 1793
BspMI ACCTGCnnnn'nnnn_
Cuts at: 254 513 1726 1940 2242 2623 254
Size: 259 1213 214 302 381 2307
Fragments arranged by size:
2307 1213 381 302 259 214
BsrBI CCG'CTC
Cuts at: 50 2968 3022 4506 4671 50
Size: 2918 54 1484 165 55
Fragments arranged by size:
2918 1484 165 55 54
BsrDI GCAATG_nn'
Cuts at: 647 2588 647
Size: 1941 2735
BsrFI r'CCGG_y
Cuts at: 2674 2855 3064 2674
Size: 181 209 4286
Fragments arranged by size:
4286 209 181
BssSI C'ACGA_G
Cuts at: 2947 3305 2947
Size: 358 4318
BstAPI GCAn_nnn'nTGC
Cuts at: 2053 2053
Size: 4676
BstBI TT'CG_AA
Cuts at: 3037 3037
Size: 4676
BstYI r'GATC_y
Cuts at: 1 243 1281 1652 1701 2168 2191 2526 2772
Size: 242 1038 371 49 467 23 335 246
Cuts at: 2772 3784 3870 3882 4228 1
Size: 1012 86 12 346 449
Fragments arranged by size:
1038 1012 467 449 371 346 335 246
242 86 49 23 12
Bsu36I CC'TnA_GG
Cuts at: 1241 1241
Size: 4676
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 2626 2807 2970 2626
Size: 181 163 4332
Fragments arranged by size:
4332 181 163
BtsI GCAGTG_nn'
Cuts at: 1919 1919
Size: 4676
DraI TTT'AAA
Cuts at: 191 1254 1443 1675 191
Size: 1063 189 232 3192
Fragments arranged by size:
3192 1063 232 189
DrdI GACnn_nn'nnGTC
Cuts at: 640 2382 3240 640
Size: 1742 858 2076
Fragments arranged by size:
2076 1742 858
EaeI y'GGCC_r
Cuts at: 494 873 2261 2435 2826 2853 4198 494
Size: 379 1388 174 391 27 1345 972
Fragments arranged by size:
1388 1345 972 391 379 174 27
EagI C'GGCC_G
Cuts at: 2261 2261
Size: 4676
EarI CTCTTCn'nnn_
Cuts at: 1189 1532 2699 2909 1189
Size: 343 1167 210 2956
Fragments arranged by size:
2956 1167 343 210
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 2884 3194 3340 4486 2884
Size: 310 146 1146 3074
Fragments arranged by size:
3074 1146 310 146
EcoICRI GAG'CTC
Cuts at: 1688 1688
Size: 4676
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 302 904 1192 2501 2933 3680 3948 302
Size: 602 288 1309 432 747 268 1030
Fragments arranged by size:
1309 1030 747 602 432 288 268
EcoNI CCTnn'n_nnAGG
Cuts at: 1058 1058
Size: 4676
EcoO109I rG'GnC_Cy
Cuts at: 598 1815 598
Size: 1217 3459
EcoRI G'AATT_C
Cuts at: 1680 1680
Size: 4676
EcoRV GAT'ATC
Cuts at: 675 675
Size: 4676
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 307 561 2695 307
Size: 254 2134 2288
Fragments arranged by size:
2288 2134 254
FspI TGC'GCA
Cuts at: 2457 2457
Size: 4676
HaeII r_GCGC'y
Cuts at: 2110 2358 3380 4359 2110
Size: 248 1022 979 2427
Fragments arranged by size:
2427 1022 979 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 2518 2548 2550 2580 2518
Size: 30 2 30 4614
Fragments arranged by size:
4614 30 30 2
HincII GTy'rAC
Cuts at: 1715 1715
Size: 4676
KasI G'GCGC_C
Cuts at: 2106 2354 2106
Size: 248 4428
KpnI G_GTAC'C
Cuts at: 187 1696 187
Size: 1509 3167
MfeI C'AATT_G
Cuts at: 1423 1423
Size: 4676
MluI A'CGCG_T
Cuts at: 4109 4109
Size: 4676
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 301 3347 3531 301
Size: 3046 184 1446
Fragments arranged by size:
3046 1446 184
MscI TGG'CCA
Cuts at: 496 875 2437 496
Size: 379 1562 2735
Fragments arranged by size:
2735 1562 379
MslI CAynn'nnrTG
Cuts at: 377 707 1318 2665 2792 377
Size: 330 611 1347 127 2261
Fragments arranged by size:
2261 1347 611 330 127
MspA1I CmG'CkG
Cuts at: 1300 1787 2101 2461 3474 3719 1300
Size: 487 314 360 1013 245 2257
Fragments arranged by size:
2257 1013 487 360 314 245
NaeI GCC'GGC
Cuts at: 2857 2857
Size: 4676
NarI GG'CG_CC
Cuts at: 2107 2355 2107
Size: 248 4428
NgoMIV G'CCGG_C
Cuts at: 2855 2855
Size: 4676
NsiI A_TGCA'T
Cuts at: 331 331
Size: 4676
NspI r_CATG'y
Cuts at: 558 1039 1729 2229 3136 558
Size: 481 690 500 907 2098
Fragments arranged by size:
2098 907 690 500 481
PciI A'CATG_T
Cuts at: 1035 3132 1035
Size: 2097 2579
PmeI GTTT'AAAC
Cuts at: 1675 1675
Size: 4676
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3815 3847 3815
Size: 32 4644
PpuMI rG'GwC_Cy
Cuts at: 598 598
Size: 4676
PsiI TTA'TAA
Cuts at: 347 347
Size: 4676
PstI C_TGCA'G
Cuts at: 1723 2408 1723
Size: 685 3991
PvuI CG_AT'CG
Cuts at: 104 104
Size: 4676
PvuII CAG'CTG
Cuts at: 1300 2101 2461 1300
Size: 801 360 3515
Fragments arranged by size:
3515 801 360
RsrII CG'GwC_CG
Cuts at: 2871 2871
Size: 4676
SacI G_AGCT'C
Cuts at: 1690 1690
Size: 4676
SalI G'TCGA_C
Cuts at: 1713 1713
Size: 4676
SapI GCTCTTCn'nnn_
Cuts at: 1532 2699 2909 1532
Size: 1167 210 3299
Fragments arranged by size:
3299 1167 210
SbfI CC_TGCA'GG
Cuts at: 1723 1723
Size: 4676
ScaI AGT'ACT
Cuts at: 4193 4193
Size: 4676
SfcI C'TryA_G
Cuts at: 33 930 1719 2404 3397 3588 33
Size: 897 789 685 993 191 1121
Fragments arranged by size:
1121 993 897 789 685 191
SfoI GGC'GCC
Cuts at: 2108 2356 2108
Size: 248 4428
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 4676
SmaI CCC'GGG
Cuts at: 1698 1698
Size: 4676
SmlI C'TyrA_G
Cuts at: 292 1045 1831 2746 3238 3500 292
Size: 753 786 915 492 262 1468
Fragments arranged by size:
1468 915 786 753 492 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 4676
SphI G_CATG'C
Cuts at: 558 1729 2229 558
Size: 1171 500 3005
Fragments arranged by size:
3005 1171 500
StyI C'CwwG_G
Cuts at: 907 953 1810 2164 907
Size: 46 857 354 3419
Fragments arranged by size:
3419 857 354 46
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 2259 2392 2851 3060 2259
Size: 133 459 209 3875
Fragments arranged by size:
3875 459 209 133
TatI w'GTAC_w
Cuts at: 926 4191 926
Size: 3265 1411
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 4193 4225 4193
Size: 32 4644
Tth111I GACn'n_nGTC
Cuts at: 2473 2473
Size: 4676
XbaI T'CTAG_A
Cuts at: 1707 1707
Size: 4676
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 617 2171 617
Size: 1554 3122
XmaI C'CCGG_G
Cuts at: 1696 1696
Size: 4676
XmnI GAAnn'nnTTC
Cuts at: 1127 1469 1127
Size: 342 4334
Enzymes that do cut:
AccI Acc65I AclI AcuI AflII AflIII AgeI AloI
AlwNI ApaLI ApoI AseI AsiSI AvaI BaeI BamHI
BanI BanII BbeI BbsI BcgI BciVI BclI BfrBI
BglI BglII BlpI Bme1580I BmrI BplI BpuEI BsaI
BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsiEI
BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI
BsrFI BssSI BstAPI BstBI BstYI Bsu36I BtgZI BtsI
DraI DrdI EaeI EagI EarI EciI EcoICRI Eco57MI
EcoNI EcoO109I EcoRI EcoRV FalI FspI HaeII Hin4I
HincII KasI KpnI MfeI MluI MmeI MscI MslI
MspA1I NaeI NarI NgoMIV NsiI NspI PciI PmeI
PpiI PpuMI PsiI PstI PvuI PvuII RsrII SacI
SalI SapI SbfI ScaI SfcI SfoI SgfI SmaI
SmlI SpeI SphI StyI TaqII TatI TstI Tth111I
XbaI XcmI XmaI XmnI
Enzymes that do not cut:
AarI AatII AfeI AhdI AleI AlfI ApaI AscI
AvrII BbvCI BmgBI BmtI BpmI Bpu10I BsgI BsiWI
BsmBI BsrGI BssHII BstEII BstXI BstZ17I BtgI ClaI
CspCI DraIII FseI FspAI HindIII HpaI NcoI NdeI
NheI NotI NruI PacI PasI PflMI PfoI PmlI
PshAI PspOMI PspXI PsrI SacII SanDI SexAI SfiI
SgrAI SnaBI SrfI SspI StuI SwaI XhoI ZraI