Restriction map for
pF1KE0013
(Circular) (Six-base) MAPSORT of: pF1KE0013.seq Check: 5390 from: 1 to: 5282
pF1KE0013 5282 bp
With 182 enzymes: SgfI *
March 2, 2010 14:30 ..
AccI GT'mk_AC
Cuts at: 2219 2320 2219
Size: 101 5181
Acc65I G'GTAC_C
Cuts at: 2298 2298
Size: 5282
AclI AA'CG_TT
Cuts at: 5122 5122
Size: 5282
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 149 511 1090 1474 3107 3539 4286 4554 149
Size: 362 579 384 1633 432 747 268 877
Fragments arranged by size:
1633 877 747 579 432 384 362 268
AflIII A'CryG_T
Cuts at: 234 2634 3738 4715 234
Size: 2400 1104 977 801
Fragments arranged by size:
2400 1104 977 801
AgeI A'CCGG_T
Cuts at: 3670 3670
Size: 5282
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1720 1720
Size: 5282
AlwNI CAG_nnn'CTG
Cuts at: 2587 4154 2587
Size: 1567 3715
ApaLI G'TGCA_C
Cuts at: 4052 4052
Size: 5282
ApoI r'AATT_y
Cuts at: 337 1246 1261 1284 1780 2286 4635 4891 337
Size: 909 15 23 496 506 2349 256 728
Fragments arranged by size:
2349 909 728 506 496 256 23 15
AseI AT'TA_AT
Cuts at: 20 1157 1439 1753 1765 20
Size: 1137 282 314 12 3537
Fragments arranged by size:
3537 1137 314 282 12
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 5282
AvaI C'yCGr_G
Cuts at: 2302 2302
Size: 5282
BamHI G'GATC_C
Cuts at: 2307 2307
Size: 5282
BanI G'GyrC_C
Cuts at: 432 1326 2298 2712 2960 2995 432
Size: 894 972 414 248 35 2719
Fragments arranged by size:
2719 972 894 414 248 35
BanII G_rGCy'C
Cuts at: 228 2296 3326 228
Size: 2068 1030 2184
Fragments arranged by size:
2184 2068 1030
BbeI G_GCGC'C
Cuts at: 2716 2964 2716
Size: 248 5034
BbsI GAAGACnn'nnnn_
Cuts at: 155 499 1355 155
Size: 344 856 4082
Fragments arranged by size:
4082 856 344
BbvCI CC'TCA_GC
Cuts at: 1985 1985
Size: 5282
BciVI GTATCCnnnnn_n'
Cuts at: 536 3180 3941 5282 536
Size: 2644 761 1341 536
Fragments arranged by size:
2644 1341 761 536
BclI T'GATC_A
Cuts at: 2802 2802
Size: 5282
BglII A'GATC_T
Cuts at: 2797 4834 2797
Size: 2037 3245
BlpI GC'TnA_GC
Cuts at: 2394 2394
Size: 5282
Bme1580I G_kGCm'C
Cuts at: 771 1238 2907 3000 4056 771
Size: 467 1669 93 1056 1997
Fragments arranged by size:
1997 1669 1056 467 93
BmrI ACTGGGnnnn_n'
Cuts at: 774 2494 2575 2910 5054 774
Size: 1720 81 335 2144 1002
Fragments arranged by size:
2144 1720 1002 335 81
BmtI G_CTAG'C
Cuts at: 545 682 545
Size: 137 5145
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3649 3649
Size: 5282
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1817 1817
Size: 5282
Bpu10I CC'TnA_GC
Cuts at: 1985 1985
Size: 5282
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1319 1924 2458 3337 3829 4127 1319
Size: 605 534 879 492 298 2474
Fragments arranged by size:
2474 879 605 534 492 298
BsaI GGTCTCn'nnnn_
Cuts at: 1726 4986 1726
Size: 3260 2022
BsaAI yAC'GTr
Cuts at: 1515 2635 1515
Size: 1120 4162
BsaBI GATnn'nnATC
Cuts at: 6 1931 2796 4833 6
Size: 1925 865 2037 455
Fragments arranged by size:
2037 1925 865 455
BsaHI Gr'CG_yC
Cuts at: 2713 2961 3663 5159 2713
Size: 248 702 1496 2836
Fragments arranged by size:
2836 1496 702 248
BsaWI w'CCGG_w
Cuts at: 528 2471 2992 3670 3944 4091 4502 528
Size: 1943 521 678 274 147 411 1308
Fragments arranged by size:
1943 1308 678 521 411 274 147
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1294 1294
Size: 5282
BseYI C'CCAG_C
Cuts at: 2707 3465 4042 4538 2707
Size: 758 577 496 3451
Fragments arranged by size:
3451 758 577 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 529 782 529
Size: 253 5029
BsiEI CG_ry'CG
Cuts at: 104 2824 2870 4078 104
Size: 2720 46 1208 1308
Fragments arranged by size:
2720 1308 1208 46
BsiHKAI G_wGCw'C
Cuts at: 228 2296 3074 3264 4056 228
Size: 2068 778 190 792 1454
Fragments arranged by size:
2068 1454 792 778 190
BsmI GAATG_Cn'
Cuts at: 692 692
Size: 5282
Bsp1286I G_dGCh'C
Cuts at: 228 771 1238 2296 2907 3000 3074 3264 3326
Size: 543 467 1058 611 93 74 190 62
Cuts at: 3326 4056 228
Size: 730 1454
Fragments arranged by size:
1454 1058 730 611 543 467 190 93
74 62
BspEI T'CCGG_A
Cuts at: 528 4502 528
Size: 3974 1308
BspHI T'CATG_A
Cuts at: 2169 4458 2169
Size: 2289 2993
BspMI ACCTGCnnnn'nnnn_
Cuts at: 2332 2546 2848 3229 2332
Size: 214 302 381 4385
Fragments arranged by size:
4385 381 302 214
BsrBI CCG'CTC
Cuts at: 50 3574 3628 5112 5277 50
Size: 3524 54 1484 165 55
Fragments arranged by size:
3524 1484 165 55 54
BsrDI GCAATG_nn'
Cuts at: 576 1553 2058 2099 3194 576
Size: 977 505 41 1095 2664
Fragments arranged by size:
2664 1095 977 505 41
BsrFI r'CCGG_y
Cuts at: 3280 3461 3670 3280
Size: 181 209 4892
Fragments arranged by size:
4892 209 181
BsrGI T'GTAC_A
Cuts at: 1665 1665
Size: 5282
BssSI C'ACGA_G
Cuts at: 3553 3911 3553
Size: 358 4924
BstAPI GCAn_nnn'nTGC
Cuts at: 2659 2659
Size: 5282
BstBI TT'CG_AA
Cuts at: 3643 3643
Size: 5282
BstYI r'GATC_y
Cuts at: 1 2230 2307 2774 2797 3132 3378 4390 4476
Size: 2229 77 467 23 335 246 1012 86
Cuts at: 4476 4488 4834 1
Size: 12 346 449
Fragments arranged by size:
2229 1012 467 449 346 335 246 86
77 23 12
BstZ17I GTA'TAC
Cuts at: 2220 2220
Size: 5282
BtgI C'CryG_G
Cuts at: 107 107
Size: 5282
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 3232 3413 3576 3232
Size: 181 163 4938
Fragments arranged by size:
4938 181 163
BtsI GCAGTG_nn'
Cuts at: 512 2525 512
Size: 2013 3269
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 2101 2136 2101
Size: 35 5247
DraI TTT'AAA
Cuts at: 815 2281 815
Size: 1466 3816
DrdI GACnn_nn'nnGTC
Cuts at: 2988 3846 2988
Size: 858 4424
EaeI y'GGCC_r
Cuts at: 1700 2109 2867 3041 3432 3459 4804 1700
Size: 409 758 174 391 27 1345 2178
Fragments arranged by size:
2178 1345 758 409 391 174 27
EagI C'GGCC_G
Cuts at: 2867 2867
Size: 5282
EarI CTCTTCn'nnn_
Cuts at: 185 3305 3515 185
Size: 3120 210 1952
Fragments arranged by size:
3120 1952 210
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 127 3490 3800 3946 5092 127
Size: 3363 310 146 1146 317
Fragments arranged by size:
3363 1146 317 310 146
EcoICRI GAG'CTC
Cuts at: 226 2294 226
Size: 2068 3214
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 149 511 1090 1474 1817 3107 3539 4286 4554
Size: 362 579 384 343 1290 432 747 268
Cuts at: 4554 149
Size: 877
Fragments arranged by size:
1290 877 747 579 432 384 362 343
268
EcoO109I rG'GnC_Cy
Cuts at: 2062 2421 2062
Size: 359 4923
EcoRI G'AATT_C
Cuts at: 1780 2286 1780
Size: 506 4776
EcoRV GAT'ATC
Cuts at: 2264 2264
Size: 5282
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 3301 3301
Size: 5282
FspI TGC'GCA
Cuts at: 3063 3063
Size: 5282
HaeII r_GCGC'y
Cuts at: 2716 2964 3986 4965 2716
Size: 248 1022 979 3033
Fragments arranged by size:
3033 1022 979 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 291 323 3124 3154 3156 3186 291
Size: 32 2801 30 2 30 2387
Fragments arranged by size:
2801 2387 32 30 30 2
HincII GTy'rAC
Cuts at: 2321 2321
Size: 5282
KasI G'GCGC_C
Cuts at: 2712 2960 2712
Size: 248 5034
KpnI G_GTAC'C
Cuts at: 2302 2302
Size: 5282
MluI A'CGCG_T
Cuts at: 4715 4715
Size: 5282
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 374 3953 4137 374
Size: 3579 184 1519
Fragments arranged by size:
3579 1519 184
MscI TGG'CCA
Cuts at: 2111 3043 2111
Size: 932 4350
MslI CAynn'nnrTG
Cuts at: 883 1387 1702 3271 3398 883
Size: 504 315 1569 127 2767
Fragments arranged by size:
2767 1569 504 315 127
MspA1I CmG'CkG
Cuts at: 283 1110 1843 2393 2707 3067 4080 4325 283
Size: 827 733 550 314 360 1013 245 1240
Fragments arranged by size:
1240 1013 827 733 550 360 314 245
NaeI GCC'GGC
Cuts at: 3463 3463
Size: 5282
NarI GG'CG_CC
Cuts at: 2713 2961 2713
Size: 248 5034
NcoI C'CATG_G
Cuts at: 107 107
Size: 5282
NdeI CA'TA_TG
Cuts at: 2196 2196
Size: 5282
NgoMIV G'CCGG_C
Cuts at: 3461 3461
Size: 5282
NheI G'CTAG_C
Cuts at: 541 678 541
Size: 137 5145
NspI r_CATG'y
Cuts at: 238 788 1242 1596 2335 2835 3742 238
Size: 550 454 354 739 500 907 1778
Fragments arranged by size:
1778 907 739 550 500 454 354
PciI A'CATG_T
Cuts at: 234 3738 234
Size: 3504 1778
PmeI GTTT'AAAC
Cuts at: 2281 2281
Size: 5282
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4421 4453 4421
Size: 32 5250
PpuMI rG'GwC_Cy
Cuts at: 2062 2062
Size: 5282
PsiI TTA'TAA
Cuts at: 416 992 1201 1997 416
Size: 576 209 796 3701
Fragments arranged by size:
3701 796 576 209
PsrI GAACnnnnnnTACnnnnnnn_nnnnn'
Cuts at: 2218 2250 2218
Size: 32 5250
PstI C_TGCA'G
Cuts at: 1325 2329 3014 1325
Size: 1004 685 3593
Fragments arranged by size:
3593 1004 685
PvuI CG_AT'CG
Cuts at: 104 104
Size: 5282
PvuII CAG'CTG
Cuts at: 1110 1843 2707 3067 1110
Size: 733 864 360 3325
Fragments arranged by size:
3325 864 733 360
RsrII CG'GwC_CG
Cuts at: 3477 3477
Size: 5282
SacI G_AGCT'C
Cuts at: 228 2296 228
Size: 2068 3214
SalI G'TCGA_C
Cuts at: 2319 2319
Size: 5282
SanDI GG'GwC_CC
Cuts at: 2062 2062
Size: 5282
SapI GCTCTTCn'nnn_
Cuts at: 3305 3515 3305
Size: 210 5072
SbfI CC_TGCA'GG
Cuts at: 1325 2329 1325
Size: 1004 4278
ScaI AGT'ACT
Cuts at: 4799 4799
Size: 5282
SfcI C'TryA_G
Cuts at: 33 131 1030 1321 1774 2076 2325 3010 4003
Size: 98 899 291 453 302 249 685 993
Cuts at: 4003 4194 33
Size: 191 1121
Fragments arranged by size:
1121 993 899 685 453 302 291 249
191 98
SfoI GGC'GCC
Cuts at: 2714 2962 2714
Size: 248 5034
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 5282
SmaI CCC'GGG
Cuts at: 2304 2304
Size: 5282
SmlI C'TyrA_G
Cuts at: 1334 1939 2437 3352 3844 4106 1334
Size: 605 498 915 492 262 2510
Fragments arranged by size:
2510 915 605 498 492 262
SpeI A'CTAG_T
Cuts at: 67 289 67
Size: 222 5060
SphI G_CATG'C
Cuts at: 788 2335 2835 788
Size: 1547 500 3235
Fragments arranged by size:
3235 1547 500
SspI AAT'ATT
Cuts at: 1197 1676 1197
Size: 479 4803
StyI C'CwwG_G
Cuts at: 107 2416 2770 107
Size: 2309 354 2619
Fragments arranged by size:
2619 2309 354
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 1685 1861 2865 2998 3457 3666 1685
Size: 176 1004 133 459 209 3301
Fragments arranged by size:
3301 1004 459 209 176 133
TatI w'GTAC_w
Cuts at: 1174 1568 1665 4797 1174
Size: 394 97 3132 1659
Fragments arranged by size:
3132 1659 394 97
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 1886 1918 4799 4831 1886
Size: 32 2881 32 2337
Fragments arranged by size:
2881 2337 32 32
Tth111I GACn'n_nGTC
Cuts at: 1359 3079 1359
Size: 1720 3562
XbaI T'CTAG_A
Cuts at: 2313 2313
Size: 5282
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2777 2777
Size: 5282
XmaI C'CCGG_G
Cuts at: 2302 2302
Size: 5282
XmnI GAAnn'nnTTC
Cuts at: 2206 2206
Size: 5282
Enzymes that do cut:
AccI Acc65I AclI AcuI AflIII AgeI AlfI AlwNI
ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII
BbeI BbsI BbvCI BciVI BclI BglII BlpI Bme1580I
BmrI BmtI BplI BpmI Bpu10I BpuEI BsaI BsaAI
BsaBI BsaHI BsaWI BseRI BseYI BsgI BsiEI BsiHKAI
BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI
BsrGI BssSI BstAPI BstBI BstYI BstZ17I BtgI BtgZI
BtsI CspCI DraI DrdI EaeI EagI EarI EciI
EcoICRI Eco57MI EcoO109I EcoRI EcoRV FalI FspI HaeII
Hin4I HincII KasI KpnI MluI MmeI MscI MslI
MspA1I NaeI NarI NcoI NdeI NgoMIV NheI NspI
PciI PmeI PpiI PpuMI PsiI PsrI PstI PvuI
PvuII RsrII SacI SalI SanDI SapI SbfI ScaI
SfcI SfoI SgfI SmaI SmlI SpeI SphI SspI
StyI TaqII TatI TstI Tth111I XbaI XcmI XmaI
XmnI
Enzymes that do not cut:
AarI AatII AfeI AflII AhdI AleI AloI ApaI
AscI AvrII BaeI BcgI BfrBI BglI BmgBI BsaXI
BsiWI BsmBI BssHII BstEII BstXI Bsu36I ClaI DraIII
EcoNI FseI FspAI HindIII HpaI MfeI NotI NruI
NsiI PacI PasI PflMI PfoI PmlI PshAI PspOMI
PspXI SacII SexAI SfiI SgrAI SnaBI SrfI StuI
SwaI XhoI ZraI