Restriction map for
pF1KE0016
(Circular) (Six-base) MAPSORT of: pF1KE0016.seq Check: 7470 from: 1 to: 5336
pF1KE0016 5336 bp
With 182 enzymes: SgfI *
March 2, 2010 14:30 ..
AatII G_ACGT'C
Cuts at: 482 482
Size: 5336
AccI GT'mk_AC
Cuts at: 327 2374 327
Size: 2047 3289
Acc65I G'GTAC_C
Cuts at: 2352 2352
Size: 5336
AclI AA'CG_TT
Cuts at: 5176 5176
Size: 5336
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 903 1253 2039 2141 3161 3593 4340 4608 903
Size: 350 786 102 1020 432 747 268 1631
Fragments arranged by size:
1631 1020 786 747 432 350 268 102
AflIII A'CryG_T
Cuts at: 2688 3792 4769 2688
Size: 1104 977 3255
Fragments arranged by size:
3255 1104 977
AgeI A'CCGG_T
Cuts at: 3724 3724
Size: 5336
AleI CACnn'nnGTG
Cuts at: 1006 1006
Size: 5336
AlwNI CAG_nnn'CTG
Cuts at: 1163 2641 4208 1163
Size: 1478 1567 2291
Fragments arranged by size:
2291 1567 1478
ApaI G_GGCC'C
Cuts at: 991 991
Size: 5336
ApaLI G'TGCA_C
Cuts at: 589 4106 589
Size: 3517 1819
ApoI r'AATT_y
Cuts at: 829 2340 4689 4945 829
Size: 1511 2349 256 1220
Fragments arranged by size:
2349 1511 1220 256
AseI AT'TA_AT
Cuts at: 20 2047 20
Size: 2027 3309
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 5336
AvaI C'yCGr_G
Cuts at: 402 1876 2356 402
Size: 1474 480 3382
Fragments arranged by size:
3382 1474 480
BamHI G'GATC_C
Cuts at: 1253 2361 1253
Size: 1108 4228
BanI G'GyrC_C
Cuts at: 2352 2766 3014 3049 2352
Size: 414 248 35 4639
Fragments arranged by size:
4639 414 248 35
BanII G_rGCy'C
Cuts at: 991 1401 2350 3380 991
Size: 410 949 1030 2947
Fragments arranged by size:
2947 1030 949 410
BbeI G_GCGC'C
Cuts at: 2770 3018 2770
Size: 248 5088
BbsI GAAGACnn'nnnn_
Cuts at: 474 1241 474
Size: 767 4569
BbvCI CC'TCA_GC
Cuts at: 1712 1712
Size: 5336
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 586 620 586
Size: 34 5302
BciVI GTATCCnnnnn_n'
Cuts at: 3234 3995 5336 3234
Size: 761 1341 3234
Fragments arranged by size:
3234 1341 761
BclI T'GATC_A
Cuts at: 2058 2856 2058
Size: 798 4538
BglI GCCn_nnn'nGGC
Cuts at: 547 1205 547
Size: 658 4678
BglII A'GATC_T
Cuts at: 1893 2851 4888 1893
Size: 958 2037 2341
Fragments arranged by size:
2341 2037 958
BlpI GC'TnA_GC
Cuts at: 2448 2448
Size: 5336
Bme1580I G_kGCm'C
Cuts at: 593 642 985 991 2961 3054 4110 593
Size: 49 343 6 1970 93 1056 1819
Fragments arranged by size:
1970 1819 1056 343 93 49 6
BmrI ACTGGGnnnn_n'
Cuts at: 2548 2629 2964 5108 2548
Size: 81 335 2144 2776
Fragments arranged by size:
2776 2144 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3703 3703
Size: 5336
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 962 990 1185 962
Size: 28 195 5113
Fragments arranged by size:
5113 195 28
Bpu10I CC'TnA_GC
Cuts at: 898 1402 1553 1712 898
Size: 504 151 159 4522
Fragments arranged by size:
4522 504 159 151
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2119 2512 3391 3883 4181 2119
Size: 393 879 492 298 3274
Fragments arranged by size:
3274 879 492 393 298
BsaI GGTCTCn'nnnn_
Cuts at: 5040 5040
Size: 5336
BsaAI yAC'GTr
Cuts at: 432 963 1908 2689 432
Size: 531 945 781 3079
Fragments arranged by size:
3079 945 781 531
BsaBI GATnn'nnATC
Cuts at: 6 131 1898 2850 4887 6
Size: 125 1767 952 2037 455
Fragments arranged by size:
2037 1767 952 455 125
BsaHI Gr'CG_yC
Cuts at: 479 550 1881 2767 3015 3717 5213 479
Size: 71 1331 886 248 702 1496 602
Fragments arranged by size:
1496 1331 886 702 602 248 71
BsaWI w'CCGG_w
Cuts at: 1826 2525 3046 3724 3998 4145 4556 1826
Size: 699 521 678 274 147 411 2606
Fragments arranged by size:
2606 699 678 521 411 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 218 248 1006 1036 1708 1738 218
Size: 30 758 30 672 30 3816
Fragments arranged by size:
3816 758 672 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 904 1729 1978 904
Size: 825 249 4262
Fragments arranged by size:
4262 825 249
BseYI C'CCAG_C
Cuts at: 789 949 1357 1446 2091 2761 3519 4096 4592
Size: 160 408 89 645 670 758 577 496
Cuts at: 4592 789
Size: 1533
Fragments arranged by size:
1533 758 670 645 577 496 408 160
89
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 433 517 644 1804 433
Size: 84 127 1160 3965
Fragments arranged by size:
3965 1160 127 84
BsiEI CG_ry'CG
Cuts at: 104 621 2878 2924 4132 104
Size: 517 2257 46 1208 1308
Fragments arranged by size:
2257 1308 1208 517 46
BsiHKAI G_wGCw'C
Cuts at: 593 1231 1530 1958 2350 3128 3318 4110 593
Size: 638 299 428 392 778 190 792 1819
Fragments arranged by size:
1819 792 778 638 428 392 299 190
BsmI GAATG_Cn'
Cuts at: 708 1086 1926 708
Size: 378 840 4118
Fragments arranged by size:
4118 840 378
Bsp1286I G_dGCh'C
Cuts at: 593 642 985 991 1231 1401 1530 1958 2350
Size: 49 343 6 240 170 129 428 392
Cuts at: 2350 2961 3054 3128 3318 3380 4110 593
Size: 611 93 74 190 62 730 1819
Fragments arranged by size:
1819 730 611 428 392 343 240 190
170 129 93 74 62 49 6
BspEI T'CCGG_A
Cuts at: 4556 4556
Size: 5336
BspHI T'CATG_A
Cuts at: 309 4512 309
Size: 4203 1133
BspMI ACCTGCnnnn'nnnn_
Cuts at: 2386 2600 2902 3283 2386
Size: 214 302 381 4439
Fragments arranged by size:
4439 381 302 214
BsrBI CCG'CTC
Cuts at: 50 3628 3682 5166 5331 50
Size: 3578 54 1484 165 55
Fragments arranged by size:
3578 1484 165 55 54
BsrDI GCAATG_nn'
Cuts at: 1430 3248 1430
Size: 1818 3518
BsrFI r'CCGG_y
Cuts at: 359 993 3334 3515 3724 359
Size: 634 2341 181 209 1971
Fragments arranged by size:
2341 1971 634 209 181
BssSI C'ACGA_G
Cuts at: 3607 3965 3607
Size: 358 4978
BstAPI GCAn_nnn'nTGC
Cuts at: 2713 2713
Size: 5336
BstBI TT'CG_AA
Cuts at: 3697 3697
Size: 5336
BstYI r'GATC_y
Cuts at: 1 1253 1893 2361 2828 2851 3186 3432 4444
Size: 1252 640 468 467 23 335 246 1012
Cuts at: 4444 4530 4542 4888 1
Size: 86 12 346 449
Fragments arranged by size:
1252 1012 640 468 467 449 346 335
246 86 23 12
BtgI C'CryG_G
Cuts at: 107 419 2172 2258 107
Size: 312 1753 86 3185
Fragments arranged by size:
3185 1753 312 86
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 3286 3467 3630 3286
Size: 181 163 4992
Fragments arranged by size:
4992 181 163
BtsI GCAGTG_nn'
Cuts at: 758 2579 758
Size: 1821 3515
DraI TTT'AAA
Cuts at: 797 2335 797
Size: 1538 3798
DraIII CAC_nnn'GTG
Cuts at: 420 599 420
Size: 179 5157
DrdI GACnn_nn'nnGTC
Cuts at: 3042 3900 3042
Size: 858 4478
EaeI y'GGCC_r
Cuts at: 110 560 618 755 1239 2219 2921 3095 3486
Size: 450 58 137 484 980 702 174 391
Cuts at: 3486 3513 4858 110
Size: 27 1345 588
Fragments arranged by size:
1345 980 702 588 484 450 391 174
137 58 27
EagI C'GGCC_G
Cuts at: 618 2921 618
Size: 2303 3033
EarI CTCTTCn'nnn_
Cuts at: 922 2025 3359 3569 922
Size: 1103 1334 210 2689
Fragments arranged by size:
2689 1334 1103 210
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 1515 3544 3854 4000 5146 1515
Size: 2029 310 146 1146 1705
Fragments arranged by size:
2029 1705 1146 310 146
EcoICRI GAG'CTC
Cuts at: 2348 2348
Size: 5336
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 903 962 990 1185 1253 2039 2141 3161 3593
Size: 59 28 195 68 786 102 1020 432
Cuts at: 3593 4340 4608 903
Size: 747 268 1631
Fragments arranged by size:
1631 1020 786 747 432 268 195 102
68 59 28
EcoO109I rG'GnC_Cy
Cuts at: 270 297 909 2475 270
Size: 27 612 1566 3131
Fragments arranged by size:
3131 1566 612 27
EcoRI G'AATT_C
Cuts at: 2340 2340
Size: 5336
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 3355 3355
Size: 5336
FspI TGC'GCA
Cuts at: 1486 3117 1486
Size: 1631 3705
HaeII r_GCGC'y
Cuts at: 2770 3018 4040 5019 2770
Size: 248 1022 979 3087
Fragments arranged by size:
3087 1022 979 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 218 250 469 501 1245 1277 1708 1740 3178
Size: 32 219 32 744 32 431 32 1438
Cuts at: 3178 3208 3210 3240 218
Size: 30 2 30 2314
Fragments arranged by size:
2314 1438 744 431 219 32 32 32
32 30 30 2
HincII GTy'rAC
Cuts at: 261 438 2375 261
Size: 177 1937 3222
Fragments arranged by size:
3222 1937 177
HindIII A'AGCT_T
Cuts at: 2123 2123
Size: 5336
HpaI GTT'AAC
Cuts at: 261 261
Size: 5336
KasI G'GCGC_C
Cuts at: 2766 3014 2766
Size: 248 5088
KpnI G_GTAC'C
Cuts at: 2356 2356
Size: 5336
MluI A'CGCG_T
Cuts at: 4769 4769
Size: 5336
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 98 2007 4007 4191 98
Size: 1909 2000 184 1243
Fragments arranged by size:
2000 1909 1243 184
MscI TGG'CCA
Cuts at: 757 2221 3097 757
Size: 1464 876 2996
Fragments arranged by size:
2996 1464 876
MslI CAynn'nnrTG
Cuts at: 112 817 1006 1042 1910 3325 3452 112
Size: 705 189 36 868 1415 127 1996
Fragments arranged by size:
1996 1415 868 705 189 127 36
MspA1I CmG'CkG
Cuts at: 1438 1490 1606 1759 2447 2761 3121 4134 4379
Size: 52 116 153 688 314 360 1013 245
Cuts at: 4379 1438
Size: 2395
Fragments arranged by size:
2395 1013 688 360 314 245 153 116
52
NaeI GCC'GGC
Cuts at: 995 3517 995
Size: 2522 2814
NarI GG'CG_CC
Cuts at: 2767 3015 2767
Size: 248 5088
NcoI C'CATG_G
Cuts at: 107 2172 107
Size: 2065 3271
NgoMIV G'CCGG_C
Cuts at: 993 3515 993
Size: 2522 2814
NspI r_CATG'y
Cuts at: 897 1041 2389 2889 3796 897
Size: 144 1348 500 907 2437
Fragments arranged by size:
2437 1348 907 500 144
PasI CC'CwG_GG
Cuts at: 291 545 291
Size: 254 5082
PciI A'CATG_T
Cuts at: 3792 3792
Size: 5336
PflMI CCAn_nnn'nTGG
Cuts at: 420 722 420
Size: 302 5034
PmeI GTTT'AAAC
Cuts at: 2335 2335
Size: 5336
PmlI CAC'GTG
Cuts at: 432 1908 432
Size: 1476 3860
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4475 4507 4475
Size: 32 5304
PpuMI rG'GwC_Cy
Cuts at: 270 297 270
Size: 27 5309
PspOMI G'GGCC_C
Cuts at: 987 987
Size: 5336
PstI C_TGCA'G
Cuts at: 509 2383 3068 509
Size: 1874 685 2777
Fragments arranged by size:
2777 1874 685
PvuI CG_AT'CG
Cuts at: 104 104
Size: 5336
PvuII CAG'CTG
Cuts at: 1606 2761 3121 1606
Size: 1155 360 3821
Fragments arranged by size:
3821 1155 360
RsrII CG'GwC_CG
Cuts at: 3531 3531
Size: 5336
SacI G_AGCT'C
Cuts at: 2350 2350
Size: 5336
SalI G'TCGA_C
Cuts at: 2373 2373
Size: 5336
SapI GCTCTTCn'nnn_
Cuts at: 3359 3569 3359
Size: 210 5126
SbfI CC_TGCA'GG
Cuts at: 2383 2383
Size: 5336
ScaI AGT'ACT
Cuts at: 4853 4853
Size: 5336
SfcI C'TryA_G
Cuts at: 33 505 1702 2379 3064 4057 4248 33
Size: 472 1197 677 685 993 191 1121
Fragments arranged by size:
1197 1121 993 685 677 472 191
SfoI GGC'GCC
Cuts at: 2768 3016 2768
Size: 248 5088
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 5336
SmaI CCC'GGG
Cuts at: 2358 2358
Size: 5336
SmlI C'TyrA_G
Cuts at: 2134 2491 3406 3898 4160 2134
Size: 357 915 492 262 3310
Fragments arranged by size:
3310 915 492 357 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 5336
SphI G_CATG'C
Cuts at: 897 1041 2389 2889 897
Size: 144 1348 500 3344
Fragments arranged by size:
3344 1348 500 144
StyI C'CwwG_G
Cuts at: 107 663 2172 2470 2824 107
Size: 556 1509 298 354 2619
Fragments arranged by size:
2619 1509 556 354 298
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 714 2919 3052 3511 3720 714
Size: 2205 133 459 209 2330
Fragments arranged by size:
2330 2205 459 209 133
TatI w'GTAC_w
Cuts at: 509 4851 509
Size: 4342 994
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 4853 4885 4853
Size: 32 5304
Tth111I GACn'n_nGTC
Cuts at: 232 3133 232
Size: 2901 2435
XbaI T'CTAG_A
Cuts at: 2211 2367 2211
Size: 156 5180
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 114 2831 114
Size: 2717 2619
XmaI C'CCGG_G
Cuts at: 2356 2356
Size: 5336
ZraI GAC'GTC
Cuts at: 480 480
Size: 5336
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflIII AgeI AleI
AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI BamHI
BanI BanII BbeI BbsI BbvCI BcgI BciVI BclI
BglI BglII BlpI Bme1580I BmrI BplI BpmI Bpu10I
BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI
BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI
BspMI BsrBI BsrDI BsrFI BssSI BstAPI BstBI BstYI
BtgI BtgZI BtsI DraI DraIII DrdI EaeI EagI
EarI EciI EcoICRI Eco57MI EcoO109I EcoRI FalI FspI
HaeII Hin4I HincII HindIII HpaI KasI KpnI MluI
MmeI MscI MslI MspA1I NaeI NarI NcoI NgoMIV
NspI PasI PciI PflMI PmeI PmlI PpiI PpuMI
PspOMI PstI PvuI PvuII RsrII SacI SalI SapI
SbfI ScaI SfcI SfoI SgfI SmaI SmlI SpeI
SphI StyI TaqII TatI TstI Tth111I XbaI XcmI
XmaI ZraI
Enzymes that do not cut:
AarI AfeI AflII AhdI AlfI AloI AscI AvrII
BaeI BfrBI BmgBI BmtI BsiWI BsmBI BsrGI BssHII
BstEII BstXI BstZ17I Bsu36I ClaI CspCI EcoNI EcoRV
FseI FspAI MfeI NdeI NheI NotI NruI NsiI
PacI PfoI PshAI PsiI PspXI PsrI SacII SanDI
SexAI SfiI SgrAI SnaBI SrfI SspI StuI SwaI
XhoI XmnI