Restriction map for
pF1KE0025
(Circular) (Six-base) MAPSORT of: pF1KE0025.seq Check: 36 from: 1 to: 4835
pF1KE0025 4835 bp
With 182 enzymes: SgfI *
April 24, 2009 13:09 ..
AccI GT'mk_AC
Cuts at: 1873 1873
Size: 4835
Acc65I G'GTAC_C
Cuts at: 1851 1851
Size: 4835
AclI AA'CG_TT
Cuts at: 4675 4675
Size: 4835
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 2660 3092 3839 4107 2660
Size: 432 747 268 3388
Fragments arranged by size:
3388 747 432 268
AflIII A'CryG_T
Cuts at: 2187 3291 4268 2187
Size: 1104 977 2754
Fragments arranged by size:
2754 1104 977
AgeI A'CCGG_T
Cuts at: 3223 3223
Size: 4835
AlwNI CAG_nnn'CTG
Cuts at: 2140 3707 2140
Size: 1567 3268
ApaLI G'TGCA_C
Cuts at: 3605 3605
Size: 4835
ApoI r'AATT_y
Cuts at: 601 1170 1196 1839 4188 4444 601
Size: 569 26 643 2349 256 992
Fragments arranged by size:
2349 992 643 569 256 26
AseI AT'TA_AT
Cuts at: 20 20
Size: 4835
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 4835
AvaI C'yCGr_G
Cuts at: 268 385 1092 1133 1739 1855 268
Size: 117 707 41 606 116 3248
Fragments arranged by size:
3248 707 606 117 116 41
BamHI G'GATC_C
Cuts at: 1860 1860
Size: 4835
BanI G'GyrC_C
Cuts at: 272 1851 2265 2513 2548 272
Size: 1579 414 248 35 2559
Fragments arranged by size:
2559 1579 414 248 35
BanII G_rGCy'C
Cuts at: 830 1746 1849 2879 830
Size: 916 103 1030 2786
Fragments arranged by size:
2786 1030 916 103
BbeI G_GCGC'C
Cuts at: 276 2269 2517 276
Size: 1993 248 2594
Fragments arranged by size:
2594 1993 248
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1594 1628 1594
Size: 34 4801
BciVI GTATCCnnnnn_n'
Cuts at: 1429 2733 3494 4835 1429
Size: 1304 761 1341 1429
Fragments arranged by size:
1429 1341 1304 761
BclI T'GATC_A
Cuts at: 702 1215 2355 702
Size: 513 1140 3182
Fragments arranged by size:
3182 1140 513
BglII A'GATC_T
Cuts at: 2350 4387 2350
Size: 2037 2798
BlpI GC'TnA_GC
Cuts at: 1947 1947
Size: 4835
Bme1580I G_kGCm'C
Cuts at: 2460 2553 3609 2460
Size: 93 1056 3686
Fragments arranged by size:
3686 1056 93
BmrI ACTGGGnnnn_n'
Cuts at: 285 1482 2047 2128 2463 4607 285
Size: 1197 565 81 335 2144 513
Fragments arranged by size:
2144 1197 565 513 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 1765 3202 1765
Size: 1437 3398
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 938 938
Size: 4835
Bpu10I CC'TnA_GC
Cuts at: 1517 1517
Size: 4835
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2011 2890 3382 3680 2011
Size: 879 492 298 3166
Fragments arranged by size:
3166 879 492 298
BsaI GGTCTCn'nnnn_
Cuts at: 268 999 4539 268
Size: 731 3540 564
Fragments arranged by size:
3540 731 564
BsaAI yAC'GTr
Cuts at: 2188 2188
Size: 4835
BsaBI GATnn'nnATC
Cuts at: 6 2349 4386 6
Size: 2343 2037 455
Fragments arranged by size:
2343 2037 455
BsaHI Gr'CG_yC
Cuts at: 273 2266 2514 3216 4712 273
Size: 1993 248 702 1496 396
Fragments arranged by size:
1993 1496 702 396 248
BsaWI w'CCGG_w
Cuts at: 806 2024 2545 3223 3497 3644 4055 806
Size: 1218 521 678 274 147 411 1586
Fragments arranged by size:
1586 1218 678 521 411 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 178 208 488 518 641 671 178
Size: 30 280 30 123 30 4342
Fragments arranged by size:
4342 280 123 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 256 1169 256
Size: 913 3922
BseYI C'CCAG_C
Cuts at: 219 2260 3018 3595 4091 219
Size: 2041 758 577 496 963
Fragments arranged by size:
2041 963 758 577 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 781 781
Size: 4835
BsiEI CG_ry'CG
Cuts at: 104 2377 2423 3631 104
Size: 2273 46 1208 1308
Fragments arranged by size:
2273 1308 1208 46
BsiHKAI G_wGCw'C
Cuts at: 233 1849 2627 2817 3609 233
Size: 1616 778 190 792 1459
Fragments arranged by size:
1616 1459 792 778 190
BsmI GAATG_Cn'
Cuts at: 1050 1050
Size: 4835
BsmBI CGTCTCn'nnnn_
Cuts at: 1322 1322
Size: 4835
Bsp1286I G_dGCh'C
Cuts at: 233 830 1746 1849 2460 2553 2627 2817 2879
Size: 597 916 103 611 93 74 190 62
Cuts at: 2879 3609 233
Size: 730 1459
Fragments arranged by size:
1459 916 730 611 597 190 103 93
74 62
BspEI T'CCGG_A
Cuts at: 806 4055 806
Size: 3249 1586
BspHI T'CATG_A
Cuts at: 699 797 4011 699
Size: 98 3214 1523
Fragments arranged by size:
3214 1523 98
BspMI ACCTGCnnnn'nnnn_
Cuts at: 1885 2099 2401 2782 1885
Size: 214 302 381 3938
Fragments arranged by size:
3938 381 302 214
BsrBI CCG'CTC
Cuts at: 50 284 3127 3181 4665 4830 50
Size: 234 2843 54 1484 165 55
Fragments arranged by size:
2843 1484 234 165 55 54
BsrDI GCAATG_nn'
Cuts at: 125 2747 125
Size: 2622 2213
BsrFI r'CCGG_y
Cuts at: 2833 3014 3223 2833
Size: 181 209 4445
Fragments arranged by size:
4445 209 181
BsrGI T'GTAC_A
Cuts at: 451 1769 451
Size: 1318 3517
BssSI C'ACGA_G
Cuts at: 3106 3464 3106
Size: 358 4477
BstAPI GCAn_nnn'nTGC
Cuts at: 1069 1693 2212 1069
Size: 624 519 3692
Fragments arranged by size:
3692 624 519
BstBI TT'CG_AA
Cuts at: 3196 3196
Size: 4835
BstYI r'GATC_y
Cuts at: 1 1860 2327 2350 2685 2931 3943 4029 4041
Size: 1859 467 23 335 246 1012 86 12
Cuts at: 4041 4387 1
Size: 346 449
Fragments arranged by size:
1859 1012 467 449 346 335 246 86
23 12
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 2785 2966 3129 2785
Size: 181 163 4491
Fragments arranged by size:
4491 181 163
BtsI GCAGTG_nn'
Cuts at: 2078 2078
Size: 4835
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 1581 1616 1581
Size: 35 4800
DraI TTT'AAA
Cuts at: 1059 1383 1834 1059
Size: 324 451 4060
Fragments arranged by size:
4060 451 324
DrdI GACnn_nn'nnGTC
Cuts at: 2541 3399 2541
Size: 858 3977
EaeI y'GGCC_r
Cuts at: 176 2420 2594 2985 3012 4357 176
Size: 2244 174 391 27 1345 654
Fragments arranged by size:
2244 1345 654 391 174 27
EagI C'GGCC_G
Cuts at: 2420 2420
Size: 4835
EarI CTCTTCn'nnn_
Cuts at: 238 307 765 2858 3068 238
Size: 69 458 2093 210 2005
Fragments arranged by size:
2093 2005 458 210 69
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 3043 3353 3499 4645 3043
Size: 310 146 1146 3233
Fragments arranged by size:
3233 1146 310 146
EcoICRI GAG'CTC
Cuts at: 1847 1847
Size: 4835
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 938 2660 3092 3839 4107 938
Size: 1722 432 747 268 1666
Fragments arranged by size:
1722 1666 747 432 268
EcoNI CCTnn'n_nnAGG
Cuts at: 1516 1516
Size: 4835
EcoO109I rG'GnC_Cy
Cuts at: 189 1618 1974 189
Size: 1429 356 3050
Fragments arranged by size:
3050 1429 356
EcoRI G'AATT_C
Cuts at: 601 1839 601
Size: 1238 3597
EcoRV GAT'ATC
Cuts at: 924 924
Size: 4835
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2854 2854
Size: 4835
FspI TGC'GCA
Cuts at: 2616 2616
Size: 4835
HaeII r_GCGC'y
Cuts at: 276 2269 2517 3539 4518 276
Size: 1993 248 1022 979 593
Fragments arranged by size:
1993 1022 979 593 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 301 333 687 719 2677 2707 2709 2739 301
Size: 32 354 32 1958 30 2 30 2397
Fragments arranged by size:
2397 1958 354 32 32 30 30 2
HincII GTy'rAC
Cuts at: 1146 1874 1146
Size: 728 4107
HindIII A'AGCT_T
Cuts at: 429 1278 429
Size: 849 3986
KasI G'GCGC_C
Cuts at: 272 2265 2513 272
Size: 1993 248 2594
Fragments arranged by size:
2594 1993 248
KpnI G_GTAC'C
Cuts at: 1855 1855
Size: 4835
MluI A'CGCG_T
Cuts at: 4268 4268
Size: 4835
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 576 3506 3690 576
Size: 2930 184 1721
Fragments arranged by size:
2930 1721 184
MscI TGG'CCA
Cuts at: 178 2596 178
Size: 2418 2417
MslI CAynn'nnrTG
Cuts at: 2824 2951 2824
Size: 127 4708
MspA1I CmG'CkG
Cuts at: 242 445 526 1946 2260 2620 3633 3878 242
Size: 203 81 1420 314 360 1013 245 1199
Fragments arranged by size:
1420 1199 1013 360 314 245 203 81
NaeI GCC'GGC
Cuts at: 3016 3016
Size: 4835
NarI GG'CG_CC
Cuts at: 273 2266 2514 273
Size: 1993 248 2594
Fragments arranged by size:
2594 1993 248
NgoMIV G'CCGG_C
Cuts at: 3014 3014
Size: 4835
NspI r_CATG'y
Cuts at: 1888 2388 3295 1888
Size: 500 907 3428
Fragments arranged by size:
3428 907 500
PciI A'CATG_T
Cuts at: 3291 3291
Size: 4835
PflMI CCAn_nnn'nTGG
Cuts at: 1357 1357
Size: 4835
PmeI GTTT'AAAC
Cuts at: 1834 1834
Size: 4835
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3974 4006 3974
Size: 32 4803
PpuMI rG'GwC_Cy
Cuts at: 189 1618 189
Size: 1429 3406
PshAI GACnn'nnGTC
Cuts at: 641 641
Size: 4835
PsiI TTA'TAA
Cuts at: 149 149
Size: 4835
PspXI vC'TCGA_Gb
Cuts at: 385 1133 1739 385
Size: 748 606 3481
Fragments arranged by size:
3481 748 606
PstI C_TGCA'G
Cuts at: 611 1882 2567 611
Size: 1271 685 2879
Fragments arranged by size:
2879 1271 685
PvuI CG_AT'CG
Cuts at: 104 104
Size: 4835
PvuII CAG'CTG
Cuts at: 445 526 2260 2620 445
Size: 81 1734 360 2660
Fragments arranged by size:
2660 1734 360 81
RsrII CG'GwC_CG
Cuts at: 3030 3030
Size: 4835
SacI G_AGCT'C
Cuts at: 1849 1849
Size: 4835
SalI G'TCGA_C
Cuts at: 1872 1872
Size: 4835
SapI GCTCTTCn'nnn_
Cuts at: 238 765 2858 3068 238
Size: 527 2093 210 2005
Fragments arranged by size:
2093 2005 527 210
SbfI CC_TGCA'GG
Cuts at: 1882 1882
Size: 4835
ScaI AGT'ACT
Cuts at: 1139 4352 1139
Size: 3213 1622
SfcI C'TryA_G
Cuts at: 33 607 1878 2563 3556 3747 33
Size: 574 1271 685 993 191 1121
Fragments arranged by size:
1271 1121 993 685 574 191
SfoI GGC'GCC
Cuts at: 274 2267 2515 274
Size: 1993 248 2594
Fragments arranged by size:
2594 1993 248
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 4835
SmaI CCC'GGG
Cuts at: 1857 1857
Size: 4835
SmlI C'TyrA_G
Cuts at: 385 1133 1739 1990 2905 3397 3659 385
Size: 748 606 251 915 492 262 1561
Fragments arranged by size:
1561 915 748 606 492 262 251
SpeI A'CTAG_T
Cuts at: 67 67
Size: 4835
SphI G_CATG'C
Cuts at: 1888 2388 1888
Size: 500 4335
SspI AAT'ATT
Cuts at: 417 1790 417
Size: 1373 3462
StuI AGG'CCT
Cuts at: 323 323
Size: 4835
StyI C'CwwG_G
Cuts at: 639 1165 1723 1969 2323 639
Size: 526 558 246 354 3151
Fragments arranged by size:
3151 558 526 354 246
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 2418 2551 3010 3219 2418
Size: 133 459 209 4034
Fragments arranged by size:
4034 459 209 133
TatI w'GTAC_w
Cuts at: 451 899 1137 1769 4350 451
Size: 448 238 632 2581 936
Fragments arranged by size:
2581 936 632 448 238
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 488 520 536 568 641 673 4352 4384 488
Size: 32 16 32 73 32 3679 32 939
Fragments arranged by size:
3679 939 73 32 32 32 32 16
Tth111I GACn'n_nGTC
Cuts at: 2632 2632
Size: 4835
XbaI T'CTAG_A
Cuts at: 1866 1866
Size: 4835
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 682 2330 682
Size: 1648 3187
XhoI C'TCGA_G
Cuts at: 385 1133 1739 385
Size: 748 606 3481
Fragments arranged by size:
3481 748 606
XmaI C'CCGG_G
Cuts at: 1855 1855
Size: 4835
Enzymes that do cut:
AccI Acc65I AclI AcuI AflIII AgeI AlwNI ApaLI
ApoI AseI AsiSI AvaI BamHI BanI BanII BbeI
BcgI BciVI BclI BglII BlpI Bme1580I BmrI BplI
BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI
BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI
Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI
BssSI BstAPI BstBI BstYI BtgZI BtsI CspCI DraI
DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI
EcoO109I EcoRI EcoRV FalI FspI HaeII Hin4I HincII
HindIII KasI KpnI MluI MmeI MscI MslI MspA1I
NaeI NarI NgoMIV NspI PciI PflMI PmeI PpiI
PpuMI PshAI PsiI PspXI PstI PvuI PvuII RsrII
SacI SalI SapI SbfI ScaI SfcI SfoI SgfI
SmaI SmlI SpeI SphI SspI StuI StyI TaqII
TatI TstI Tth111I XbaI XcmI XhoI XmaI
Enzymes that do not cut:
AarI AatII AfeI AflII AhdI AleI AlfI AloI
ApaI AscI AvrII BaeI BbsI BbvCI BfrBI BglI
BmgBI BmtI BsiWI BssHII BstEII BstXI BstZ17I Bsu36I
BtgI ClaI DraIII FseI FspAI HpaI MfeI NcoI
NdeI NheI NotI NruI NsiI PacI PasI PfoI
PmlI PspOMI PsrI SacII SanDI SexAI SfiI SgrAI
SnaBI SrfI SwaI XmnI ZraI