Restriction map for
pF1KE0028
(Circular) (Six-base) MAPSORT of: pF1KE0028.seq Check: 7984 from: 1 to: 5354
pF1KE0028 5354 bp
With 182 enzymes: SgfI *
April 24, 2009 13:09 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 258 258
Size: 5354
AatII G_ACGT'C
Cuts at: 1024 1024
Size: 5354
AccI GT'mk_AC
Cuts at: 398 1928 2392 398
Size: 1530 464 3360
Fragments arranged by size:
3360 1530 464
Acc65I G'GTAC_C
Cuts at: 2370 2370
Size: 5354
AclI AA'CG_TT
Cuts at: 5194 5194
Size: 5354
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 309 754 3179 3611 4358 4626 309
Size: 445 2425 432 747 268 1037
Fragments arranged by size:
2425 1037 747 445 432 268
AflIII A'CryG_T
Cuts at: 2155 2706 3810 4787 2155
Size: 551 1104 977 2722
Fragments arranged by size:
2722 1104 977 551
AgeI A'CCGG_T
Cuts at: 3742 3742
Size: 5354
AhdI GACnn_n'nnGTC
Cuts at: 1020 1020
Size: 5354
AleI CACnn'nnGTG
Cuts at: 2012 2012
Size: 5354
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1750 1750
Size: 5354
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 208 240 1972 2004 208
Size: 32 1732 32 3558
Fragments arranged by size:
3558 1732 32 32
AlwNI CAG_nnn'CTG
Cuts at: 625 1195 1261 2659 4226 625
Size: 570 66 1398 1567 1753
Fragments arranged by size:
1753 1567 1398 570 66
ApaI G_GGCC'C
Cuts at: 952 1914 2062 952
Size: 962 148 4244
Fragments arranged by size:
4244 962 148
ApaLI G'TGCA_C
Cuts at: 4124 4124
Size: 5354
ApoI r'AATT_y
Cuts at: 2358 4707 4963 2358
Size: 2349 256 2749
Fragments arranged by size:
2749 2349 256
AseI AT'TA_AT
Cuts at: 20 20
Size: 5354
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 5354
AvaI C'yCGr_G
Cuts at: 1241 1282 2374 1241
Size: 41 1092 4221
Fragments arranged by size:
4221 1092 41
AvrII C'CTAG_G
Cuts at: 2226 2226
Size: 5354
BamHI G'GATC_C
Cuts at: 1782 2379 1782
Size: 597 4757
BanI G'GyrC_C
Cuts at: 1000 1100 2370 2784 3032 3067 1000
Size: 100 1270 414 248 35 3287
Fragments arranged by size:
3287 1270 414 248 100 35
BanII G_rGCy'C
Cuts at: 925 952 970 1202 1214 1914 2062 2244 2368
Size: 27 18 232 12 700 148 182 124
Cuts at: 2368 3398 925
Size: 1030 2881
Fragments arranged by size:
2881 1030 700 232 182 148 124 27
18 12
BbeI G_GCGC'C
Cuts at: 1104 2788 3036 1104
Size: 1684 248 3422
Fragments arranged by size:
3422 1684 248
BbvCI CC'TCA_GC
Cuts at: 1051 1051
Size: 5354
BciVI GTATCCnnnnn_n'
Cuts at: 2037 2071 3252 4013 5354 2037
Size: 34 1181 761 1341 2037
Fragments arranged by size:
2037 1341 1181 761 34
BclI T'GATC_A
Cuts at: 2874 2874
Size: 5354
BglI GCCn_nnn'nGGC
Cuts at: 2231 2231
Size: 5354
BglII A'GATC_T
Cuts at: 2869 4906 2869
Size: 2037 3317
BlpI GC'TnA_GC
Cuts at: 1196 2466 1196
Size: 1270 4084
Bme1580I G_kGCm'C
Cuts at: 952 1003 1914 2062 2979 3072 4128 952
Size: 51 911 148 917 93 1056 2178
Fragments arranged by size:
2178 1056 917 911 148 93 51
BmrI ACTGGGnnnn_n'
Cuts at: 864 1076 1149 2566 2647 2982 5126 864
Size: 212 73 1417 81 335 2144 1092
Fragments arranged by size:
2144 1417 1092 335 212 81 73
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 1155 1947 3721 1155
Size: 792 1774 2788
Fragments arranged by size:
2788 1774 792
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 448 1753 1926 2230 2283 448
Size: 1305 173 304 53 3519
Fragments arranged by size:
3519 1305 304 173 53
Bpu10I CC'TnA_GC
Cuts at: 431 595 944 1051 431
Size: 164 349 107 4734
Fragments arranged by size:
4734 349 164 107
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 912 2005 2241 2530 3409 3901 4199 912
Size: 1093 236 289 879 492 298 2067
Fragments arranged by size:
2067 1093 879 492 298 289 236
BsaI GGTCTCn'nnnn_
Cuts at: 5058 5058
Size: 5354
BsaAI yAC'GTr
Cuts at: 2707 2707
Size: 5354
BsaBI GATnn'nnATC
Cuts at: 6 2868 4905 6
Size: 2862 2037 455
Fragments arranged by size:
2862 2037 455
BsaHI Gr'CG_yC
Cuts at: 194 1021 1101 1859 2174 2785 3033 3735 5231
Size: 827 80 758 315 611 248 702 1496
Cuts at: 5231 194
Size: 317
Fragments arranged by size:
1496 827 758 702 611 317 315 248
80
BsaWI w'CCGG_w
Cuts at: 1024 1075 2543 3064 3742 4016 4163 4574 1024
Size: 51 1468 521 678 274 147 411 1804
Fragments arranged by size:
1804 1468 678 521 411 274 147 51
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 208 238 208
Size: 30 5324
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 757 944 1039 1286 1327 1383 757
Size: 187 95 247 41 56 4728
Fragments arranged by size:
4728 247 187 95 56 41
BseYI C'CCAG_C
Cuts at: 1302 1509 2187 2779 3537 4114 4610 1302
Size: 207 678 592 758 577 496 2046
Fragments arranged by size:
2046 758 678 592 577 496 207
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 568 864 1181 1635 568
Size: 296 317 454 4287
Fragments arranged by size:
4287 454 317 296
BsiEI CG_ry'CG
Cuts at: 104 2896 2942 4150 104
Size: 2792 46 1208 1308
Fragments arranged by size:
2792 1308 1208 46
BsiHKAI G_wGCw'C
Cuts at: 970 1214 2269 2368 3146 3336 4128 970
Size: 244 1055 99 778 190 792 2196
Fragments arranged by size:
2196 1055 792 778 244 190 99
BsmI GAATG_Cn'
Cuts at: 861 861
Size: 5354
BsmBI CGTCTCn'nnnn_
Cuts at: 1540 1540
Size: 5354
Bsp1286I G_dGCh'C
Cuts at: 925 952 970 1003 1202 1214 1914 2062 2244
Size: 27 18 33 199 12 700 148 182
Cuts at: 2244 2269 2368 2979 3072 3146 3336 3398 4128
Size: 25 99 611 93 74 190 62 730
Cuts at: 4128 925
Size: 2151
Fragments arranged by size:
2151 730 700 611 199 190 182 148
99 93 74 62 33 27 25 18
12
BspEI T'CCGG_A
Cuts at: 1024 4574 1024
Size: 3550 1804
BspHI T'CATG_A
Cuts at: 4530 4530
Size: 5354
BspMI ACCTGCnnnn'nnnn_
Cuts at: 161 258 1841 2085 2296 2404 2618 2920 3301
Size: 97 1583 244 211 108 214 302 381
Cuts at: 3301 161
Size: 2214
Fragments arranged by size:
2214 1583 381 302 244 214 211 108
97
BsrBI CCG'CTC
Cuts at: 50 3646 3700 5184 5349 50
Size: 3596 54 1484 165 55
Fragments arranged by size:
3596 1484 165 55 54
BsrDI GCAATG_nn'
Cuts at: 709 1793 3266 709
Size: 1084 1473 2797
Fragments arranged by size:
2797 1473 1084
BsrFI r'CCGG_y
Cuts at: 1091 3352 3533 3742 1091
Size: 2261 181 209 2703
Fragments arranged by size:
2703 2261 209 181
BsrGI T'GTAC_A
Cuts at: 1807 1964 1807
Size: 157 5197
BssSI C'ACGA_G
Cuts at: 878 3625 3983 878
Size: 2747 358 2249
Fragments arranged by size:
2747 2249 358
BstAPI GCAn_nnn'nTGC
Cuts at: 180 264 2731 180
Size: 84 2467 2803
Fragments arranged by size:
2803 2467 84
BstBI TT'CG_AA
Cuts at: 3715 3715
Size: 5354
BstEII G'GTnAC_C
Cuts at: 2250 2250
Size: 5354
BstXI CCAn_nnnn'nTGG
Cuts at: 2014 2334 2014
Size: 320 5034
BstYI r'GATC_y
Cuts at: 1 1782 2379 2846 2869 3204 3450 4462 4548
Size: 1781 597 467 23 335 246 1012 86
Cuts at: 4548 4560 4906 1
Size: 12 346 449
Fragments arranged by size:
1781 1012 597 467 449 346 335 246
86 23 12
Bsu36I CC'TnA_GG
Cuts at: 1262 1262
Size: 5354
BtgI C'CryG_G
Cuts at: 107 119 162 455 836 1113 2054 2255 2273
Size: 12 43 293 381 277 941 201 18
Cuts at: 2273 2303 107
Size: 30 3158
Fragments arranged by size:
3158 941 381 293 277 201 43 30
18 12
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 1912 2210 3304 3485 3648 1912
Size: 298 1094 181 163 3618
Fragments arranged by size:
3618 1094 298 181 163
BtsI GCAGTG_nn'
Cuts at: 178 1344 1521 2267 2597 178
Size: 1166 177 746 330 2935
Fragments arranged by size:
2935 1166 746 330 177
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 2172 2207 2172
Size: 35 5319
DraI TTT'AAA
Cuts at: 2353 2353
Size: 5354
DraIII CAC_nnn'GTG
Cuts at: 880 880
Size: 5354
DrdI GACnn_nn'nnGTC
Cuts at: 139 3060 3918 139
Size: 2921 858 1575
Fragments arranged by size:
2921 1575 858
EaeI y'GGCC_r
Cuts at: 971 1061 2232 2939 3113 3504 3531 4876 971
Size: 90 1171 707 174 391 27 1345 1449
Fragments arranged by size:
1449 1345 1171 707 391 174 90 27
EagI C'GGCC_G
Cuts at: 2939 2939
Size: 5354
EarI CTCTTCn'nnn_
Cuts at: 163 328 752 758 913 1036 2125 3377 3587
Size: 165 424 6 155 123 1089 1252 210
Cuts at: 3587 163
Size: 1930
Fragments arranged by size:
1930 1252 1089 424 210 165 155 123
6
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 127 223 1267 3562 3872 4018 5164 127
Size: 96 1044 2295 310 146 1146 317
Fragments arranged by size:
2295 1146 1044 317 310 146 96
EcoICRI GAG'CTC
Cuts at: 968 1212 2366 968
Size: 244 1154 3956
Fragments arranged by size:
3956 1154 244
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 309 448 754 1753 1926 2230 2283 3179 3611
Size: 139 306 999 173 304 53 896 432
Cuts at: 3611 4358 4626 309
Size: 747 268 1037
Fragments arranged by size:
1037 999 896 747 432 306 304 268
173 139 53
EcoO109I rG'GnC_Cy
Cuts at: 948 949 1014 1258 1628 1910 2059 2285 2493
Size: 1 65 244 370 282 149 226 208
Cuts at: 2493 948
Size: 3809
Fragments arranged by size:
3809 370 282 244 226 208 149 65
1
EcoRI G'AATT_C
Cuts at: 2358 2358
Size: 5354
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 3373 3373
Size: 5354
FspI TGC'GCA
Cuts at: 3135 3135
Size: 5354
HaeII r_GCGC'y
Cuts at: 1104 2788 3036 4058 5037 1104
Size: 1684 248 1022 979 1421
Fragments arranged by size:
1684 1421 1022 979 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 341 373 1006 1038 3196 3226 3228 3258 341
Size: 32 633 32 2158 30 2 30 2437
Fragments arranged by size:
2437 2158 633 32 32 30 30 2
HincII GTy'rAC
Cuts at: 2393 2393
Size: 5354
HindIII A'AGCT_T
Cuts at: 700 700
Size: 5354
KasI G'GCGC_C
Cuts at: 1100 2784 3032 1100
Size: 1684 248 3422
Fragments arranged by size:
3422 1684 248
KpnI G_GTAC'C
Cuts at: 2374 2374
Size: 5354
MluI A'CGCG_T
Cuts at: 4787 4787
Size: 5354
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 801 827 4025 4209 801
Size: 26 3198 184 1946
Fragments arranged by size:
3198 1946 184 26
MscI TGG'CCA
Cuts at: 1063 2234 3115 1063
Size: 1171 881 3302
Fragments arranged by size:
3302 1171 881
MslI CAynn'nnrTG
Cuts at: 1405 2012 2272 3343 3470 1405
Size: 607 260 1071 127 3289
Fragments arranged by size:
3289 1071 607 260 127
MspA1I CmG'CkG
Cuts at: 164 189 252 1055 1098 1464 1852 2084 2465
Size: 25 63 803 43 366 388 232 381
Cuts at: 2465 2779 3139 4152 4397 164
Size: 314 360 1013 245 1121
Fragments arranged by size:
1121 1013 803 388 381 366 360 314
245 232 63 43 25
NaeI GCC'GGC
Cuts at: 3535 3535
Size: 5354
NarI GG'CG_CC
Cuts at: 1101 2785 3033 1101
Size: 1684 248 3422
Fragments arranged by size:
3422 1684 248
NcoI C'CATG_G
Cuts at: 107 836 1113 2273 2303 107
Size: 729 277 1160 30 3158
Fragments arranged by size:
3158 1160 729 277 30
NgoMIV G'CCGG_C
Cuts at: 3533 3533
Size: 5354
NspI r_CATG'y
Cuts at: 618 1593 1881 2151 2159 2407 2907 3814 618
Size: 975 288 270 8 248 500 907 2158
Fragments arranged by size:
2158 975 907 500 288 270 248 8
PasI CC'CwG_GG
Cuts at: 2108 2108
Size: 5354
PciI A'CATG_T
Cuts at: 2155 3810 2155
Size: 1655 3699
PfoI T'CCnGG_A
Cuts at: 143 143
Size: 5354
PmeI GTTT'AAAC
Cuts at: 2353 2353
Size: 5354
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 208 240 4493 4525 208
Size: 32 4253 32 1037
Fragments arranged by size:
4253 1037 32 32
PpuMI rG'GwC_Cy
Cuts at: 1014 2285 1014
Size: 1271 4083
PspOMI G'GGCC_C
Cuts at: 948 1910 2058 948
Size: 962 148 4244
Fragments arranged by size:
4244 962 148
PsrI GAACnnnnnnTACnnnnnnn_nnnnn'
Cuts at: 1948 1980 1948
Size: 32 5322
PstI C_TGCA'G
Cuts at: 257 1109 1351 2401 3086 257
Size: 852 242 1050 685 2525
Fragments arranged by size:
2525 1050 852 685 242
PvuI CG_AT'CG
Cuts at: 104 104
Size: 5354
PvuII CAG'CTG
Cuts at: 189 252 1852 2779 3139 189
Size: 63 1600 927 360 2404
Fragments arranged by size:
2404 1600 927 360 63
RsrII CG'GwC_CG
Cuts at: 3549 3549
Size: 5354
SacI G_AGCT'C
Cuts at: 970 1214 2368 970
Size: 244 1154 3956
Fragments arranged by size:
3956 1154 244
SacII CC_GC'GG
Cuts at: 165 165
Size: 5354
SalI G'TCGA_C
Cuts at: 2391 2391
Size: 5354
SapI GCTCTTCn'nnn_
Cuts at: 163 328 913 1036 3377 3587 163
Size: 165 585 123 2341 210 1930
Fragments arranged by size:
2341 1930 585 210 165 123
SbfI CC_TGCA'GG
Cuts at: 1109 2401 1109
Size: 1292 4062
ScaI AGT'ACT
Cuts at: 4871 4871
Size: 5354
SexAI A'CCwGG_T
Cuts at: 665 665
Size: 5354
SfcI C'TryA_G
Cuts at: 33 253 1105 1347 2397 3082 4075 4266 33
Size: 220 852 242 1050 685 993 191 1121
Fragments arranged by size:
1121 1050 993 852 685 242 220 191
SfoI GGC'GCC
Cuts at: 1102 2786 3034 1102
Size: 1684 248 3422
Fragments arranged by size:
3422 1684 248
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 5354
SmaI CCC'GGG
Cuts at: 2376 2376
Size: 5354
SmlI C'TyrA_G
Cuts at: 927 2020 2220 2509 3424 3916 4178 927
Size: 1093 200 289 915 492 262 2103
Fragments arranged by size:
2103 1093 915 492 289 262 200
SpeI A'CTAG_T
Cuts at: 67 67
Size: 5354
SphI G_CATG'C
Cuts at: 618 2407 2907 618
Size: 1789 500 3065
Fragments arranged by size:
3065 1789 500
StuI AGG'CCT
Cuts at: 769 769
Size: 5354
StyI C'CwwG_G
Cuts at: 107 770 836 1004 1031 1113 2226 2273 2303
Size: 663 66 168 27 82 1113 47 30
Cuts at: 2303 2488 2842 107
Size: 185 354 2619
Fragments arranged by size:
2619 1113 663 354 185 168 82 66
47 30 27
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 2179 2937 3070 3529 3738 2179
Size: 758 133 459 209 3795
Fragments arranged by size:
3795 758 459 209 133
TatI w'GTAC_w
Cuts at: 1807 1964 2033 4869 1807
Size: 157 69 2836 2292
Fragments arranged by size:
2836 2292 157 69
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 1318 1350 4871 4903 1318
Size: 32 3521 32 1769
Fragments arranged by size:
3521 1769 32 32
Tth111I GACn'n_nGTC
Cuts at: 3151 3151
Size: 5354
XbaI T'CTAG_A
Cuts at: 2385 2385
Size: 5354
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2849 2849
Size: 5354
XmaI C'CCGG_G
Cuts at: 2374 2374
Size: 5354
XmnI GAAnn'nnTTC
Cuts at: 223 223
Size: 5354
ZraI GAC'GTC
Cuts at: 1022 1022
Size: 5354
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AflIII AgeI
AhdI AleI AlfI AloI AlwNI ApaI ApaLI ApoI
AseI AsiSI AvaI AvrII BamHI BanI BanII BbeI
BbvCI BciVI BclI BglI BglII BlpI Bme1580I BmrI
BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI
BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI
BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI
BsrGI BssSI BstAPI BstBI BstEII BstXI BstYI Bsu36I
BtgI BtgZI BtsI CspCI DraI DraIII DrdI EaeI
EagI EarI EciI EcoICRI Eco57MI EcoO109I EcoRI FalI
FspI HaeII Hin4I HincII HindIII KasI KpnI MluI
MmeI MscI MslI MspA1I NaeI NarI NcoI NgoMIV
NspI PasI PciI PfoI PmeI PpiI PpuMI PspOMI
PsrI PstI PvuI PvuII RsrII SacI SacII SalI
SapI SbfI ScaI SexAI SfcI SfoI SgfI SmaI
SmlI SpeI SphI StuI StyI TaqII TatI TstI
Tth111I XbaI XcmI XmaI XmnI ZraI
Enzymes that do not cut:
AfeI AflII AscI BaeI BbsI BcgI BfrBI BmgBI
BmtI BsiWI BssHII BstZ17I ClaI EcoNI EcoRV FseI
FspAI HpaI MfeI NdeI NheI NotI NruI NsiI
PacI PflMI PmlI PshAI PsiI PspXI SanDI SfiI
SgrAI SnaBI SrfI SspI SwaI XhoI