Restriction map for
pF1KE0032
(Circular) (Six-base) MAPSORT of: pF1KE0032.seq Check: 6964 from: 1 to: 4934
pF1KE0032 4934 bp
With 182 enzymes: SgfI *
April 24, 2009 13:09 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 408 408
Size: 4934
AccI GT'mk_AC
Cuts at: 585 1972 585
Size: 1387 3547
Acc65I G'GTAC_C
Cuts at: 1950 1950
Size: 4934
AclI AA'CG_TT
Cuts at: 4774 4774
Size: 4934
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 837 1861 1928 2759 3191 3938 4206 837
Size: 1024 67 831 432 747 268 1565
Fragments arranged by size:
1565 1024 831 747 432 268 67
AflIII A'CryG_T
Cuts at: 2286 3390 4367 2286
Size: 1104 977 2853
Fragments arranged by size:
2853 1104 977
AgeI A'CCGG_T
Cuts at: 3322 3322
Size: 4934
AhdI GACnn_n'nnGTC
Cuts at: 654 654
Size: 4934
AleI CACnn'nnGTG
Cuts at: 601 743 997 1117 601
Size: 142 254 120 4418
Fragments arranged by size:
4418 254 142 120
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 555 587 555
Size: 32 4902
AlwNI CAG_nnn'CTG
Cuts at: 246 423 1275 2239 3806 246
Size: 177 852 964 1567 1374
Fragments arranged by size:
1567 1374 964 852 177
ApaI G_GGCC'C
Cuts at: 1593 1593
Size: 4934
ApaLI G'TGCA_C
Cuts at: 3704 3704
Size: 4934
ApoI r'AATT_y
Cuts at: 479 1333 1675 1938 4287 4543 479
Size: 854 342 263 2349 256 870
Fragments arranged by size:
2349 870 854 342 263 256
AseI AT'TA_AT
Cuts at: 20 20
Size: 4934
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 4934
AvaI C'yCGr_G
Cuts at: 234 1586 1897 1954 234
Size: 1352 311 57 3214
Fragments arranged by size:
3214 1352 311 57
BamHI G'GATC_C
Cuts at: 734 1494 1959 734
Size: 760 465 3709
Fragments arranged by size:
3709 760 465
BanI G'GyrC_C
Cuts at: 448 1262 1950 2364 2612 2647 448
Size: 814 688 414 248 35 2735
Fragments arranged by size:
2735 814 688 414 248 35
BanII G_rGCy'C
Cuts at: 533 1593 1839 1898 1948 2978 533
Size: 1060 246 59 50 1030 2489
Fragments arranged by size:
2489 1060 1030 246 59 50
BbeI G_GCGC'C
Cuts at: 452 2368 2616 452
Size: 1916 248 2770
Fragments arranged by size:
2770 1916 248
BbsI GAAGACnn'nnnn_
Cuts at: 995 1242 995
Size: 247 4687
BbvCI CC'TCA_GC
Cuts at: 676 1465 676
Size: 789 4145
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 298 332 298
Size: 34 4900
BciVI GTATCCnnnnn_n'
Cuts at: 2832 3593 4934 2832
Size: 761 1341 2832
Fragments arranged by size:
2832 1341 761
BclI T'GATC_A
Cuts at: 2454 2454
Size: 4934
BglI GCCn_nnn'nGGC
Cuts at: 707 1178 1405 1629 1773 707
Size: 471 227 224 144 3868
Fragments arranged by size:
3868 471 227 224 144
BglII A'GATC_T
Cuts at: 2449 4486 2449
Size: 2037 2897
BlpI GC'TnA_GC
Cuts at: 328 1095 1699 2046 328
Size: 767 604 347 3216
Fragments arranged by size:
3216 767 604 347
Bme1580I G_kGCm'C
Cuts at: 1593 2559 2652 3708 1593
Size: 966 93 1056 2819
Fragments arranged by size:
2819 1056 966 93
BmrI ACTGGGnnnn_n'
Cuts at: 178 894 1731 2146 2227 2562 4706 178
Size: 716 837 415 81 335 2144 406
Fragments arranged by size:
2144 837 716 415 406 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3301 3301
Size: 4934
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 881 1840 881
Size: 959 3975
Bpu10I CC'TnA_GC
Cuts at: 527 676 1465 527
Size: 149 789 3996
Fragments arranged by size:
3996 789 149
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1618 2110 2989 3481 3779 1618
Size: 492 879 492 298 2773
Fragments arranged by size:
2773 879 492 492 298
BsaI GGTCTCn'nnnn_
Cuts at: 277 4638 277
Size: 4361 573
BsaAI yAC'GTr
Cuts at: 2287 2287
Size: 4934
BsaBI GATnn'nnATC
Cuts at: 6 2448 4485 6
Size: 2442 2037 455
Fragments arranged by size:
2442 2037 455
BsaHI Gr'CG_yC
Cuts at: 449 2365 2613 3315 4811 449
Size: 1916 248 702 1496 572
Fragments arranged by size:
1916 1496 702 572 248
BsaWI w'CCGG_w
Cuts at: 398 2123 2644 3322 3596 3743 4154 398
Size: 1725 521 678 274 147 411 1178
Fragments arranged by size:
1725 1178 678 521 411 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 598 628 598
Size: 30 4904
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1207 1486 1705 1807 1915 1207
Size: 279 219 102 108 4226
Fragments arranged by size:
4226 279 219 108 102
BseYI C'CCAG_C
Cuts at: 201 423 917 1624 2359 3117 3694 4190 201
Size: 222 494 707 735 758 577 496 945
Fragments arranged by size:
945 758 735 707 577 496 494 222
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 292 893 1541 292
Size: 601 648 3685
Fragments arranged by size:
3685 648 601
BsiEI CG_ry'CG
Cuts at: 104 2476 2522 3730 104
Size: 2372 46 1208 1308
Fragments arranged by size:
2372 1308 1208 46
BsiHKAI G_wGCw'C
Cuts at: 1898 1948 2726 2916 3708 1898
Size: 50 778 190 792 3124
Fragments arranged by size:
3124 792 778 190 50
Bsp1286I G_dGCh'C
Cuts at: 533 1593 1839 1898 1948 2559 2652 2726 2916
Size: 1060 246 59 50 611 93 74 190
Cuts at: 2916 2978 3708 533
Size: 62 730 1759
Fragments arranged by size:
1759 1060 730 611 246 190 93 74
62 59 50
BspEI T'CCGG_A
Cuts at: 398 4154 398
Size: 3756 1178
BspHI T'CATG_A
Cuts at: 1358 4110 1358
Size: 2752 2182
BspMI ACCTGCnnnn'nnnn_
Cuts at: 408 725 1469 1811 1984 2198 2500 2881 408
Size: 317 744 342 173 214 302 381 2461
Fragments arranged by size:
2461 744 381 342 317 302 214 173
BsrBI CCG'CTC
Cuts at: 50 125 3226 3280 4764 4929 50
Size: 75 3101 54 1484 165 55
Fragments arranged by size:
3101 1484 165 75 55 54
BsrDI GCAATG_nn'
Cuts at: 1085 2846 1085
Size: 1761 3173
BsrFI r'CCGG_y
Cuts at: 269 690 720 877 2932 3113 3322 269
Size: 421 30 157 2055 181 209 1881
Fragments arranged by size:
2055 1881 421 209 181 157 30
BssSI C'ACGA_G
Cuts at: 993 3205 3563 993
Size: 2212 358 2364
Fragments arranged by size:
2364 2212 358
BstAPI GCAn_nnn'nTGC
Cuts at: 1803 2311 1803
Size: 508 4426
BstBI TT'CG_AA
Cuts at: 3295 3295
Size: 4934
BstEII G'GTnAC_C
Cuts at: 1456 1456
Size: 4934
BstXI CCAn_nnnn'nTGG
Cuts at: 1119 1119
Size: 4934
BstYI r'GATC_y
Cuts at: 1 734 1364 1494 1886 1959 2426 2449 2784
Size: 733 630 130 392 73 467 23 335
Cuts at: 2784 3030 4042 4128 4140 4486 1
Size: 246 1012 86 12 346 449
Fragments arranged by size:
1012 733 630 467 449 392 346 335
246 130 86 73 23 12
BtgI C'CryG_G
Cuts at: 738 1041 1112 738
Size: 303 71 4560
Fragments arranged by size:
4560 303 71
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 2884 3065 3228 2884
Size: 181 163 4590
Fragments arranged by size:
4590 181 163
BtsI GCAGTG_nn'
Cuts at: 219 608 2177 219
Size: 389 1569 2976
Fragments arranged by size:
2976 1569 389
DraI TTT'AAA
Cuts at: 1933 1933
Size: 4934
DrdI GACnn_nn'nnGTC
Cuts at: 117 2640 3498 117
Size: 2523 858 1553
Fragments arranged by size:
2523 1553 858
EaeI y'GGCC_r
Cuts at: 575 1121 1774 2519 2693 3084 3111 4456 575
Size: 546 653 745 174 391 27 1345 1053
Fragments arranged by size:
1345 1053 745 653 546 391 174 27
EagI C'GGCC_G
Cuts at: 2519 2519
Size: 4934
EarI CTCTTCn'nnn_
Cuts at: 1042 1682 1904 2957 3167 1042
Size: 640 222 1053 210 2809
Fragments arranged by size:
2809 1053 640 222 210
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 256 1549 3142 3452 3598 4744 256
Size: 1293 1593 310 146 1146 446
Fragments arranged by size:
1593 1293 1146 446 310 146
EcoICRI GAG'CTC
Cuts at: 1896 1946 1896
Size: 50 4884
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 837 881 1840 1861 1928 2759 3191 3938 4206
Size: 44 959 21 67 831 432 747 268
Cuts at: 4206 837
Size: 1565
Fragments arranged by size:
1565 959 831 747 432 268 67 44
21
EcoO109I rG'GnC_Cy
Cuts at: 591 1158 1590 2073 591
Size: 567 432 483 3452
Fragments arranged by size:
3452 567 483 432
EcoRI G'AATT_C
Cuts at: 479 1333 1938 479
Size: 854 605 3475
Fragments arranged by size:
3475 854 605
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2953 2953
Size: 4934
FspI TGC'GCA
Cuts at: 2715 2715
Size: 4934
HaeII r_GCGC'y
Cuts at: 452 1876 2368 2616 3638 4617 452
Size: 1424 492 248 1022 979 769
Fragments arranged by size:
1424 1022 979 769 492 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 640 672 1093 1125 1428 1460 2776 2806 2808
Size: 32 421 32 303 32 1316 30 2
Cuts at: 2808 2838 640
Size: 30 2736
Fragments arranged by size:
2736 1316 421 303 32 32 32 30
30 2
HincII GTy'rAC
Cuts at: 1149 1323 1427 1973 1149
Size: 174 104 546 4110
Fragments arranged by size:
4110 546 174 104
HpaI GTT'AAC
Cuts at: 1427 1427
Size: 4934
KasI G'GCGC_C
Cuts at: 448 2364 2612 448
Size: 1916 248 2770
Fragments arranged by size:
2770 1916 248
KpnI G_GTAC'C
Cuts at: 1954 1954
Size: 4934
MluI A'CGCG_T
Cuts at: 4367 4367
Size: 4934
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 671 3605 3789 671
Size: 2934 184 1816
Fragments arranged by size:
2934 1816 184
MscI TGG'CCA
Cuts at: 1123 2695 1123
Size: 1572 3362
MslI CAynn'nnrTG
Cuts at: 601 701 743 997 1117 1546 2923 3050 601
Size: 100 42 254 120 429 1377 127 2485
Fragments arranged by size:
2485 1377 429 254 127 120 100 42
MspA1I CmG'CkG
Cuts at: 171 510 1099 1628 1779 1809 2045 2359 2719
Size: 339 589 529 151 30 236 314 360
Cuts at: 2719 3732 3977 171
Size: 1013 245 1128
Fragments arranged by size:
1128 1013 589 529 360 339 314 245
236 151 30
NaeI GCC'GGC
Cuts at: 271 692 3115 271
Size: 421 2423 2090
Fragments arranged by size:
2423 2090 421
NarI GG'CG_CC
Cuts at: 449 2365 2613 449
Size: 1916 248 2770
Fragments arranged by size:
2770 1916 248
NgoMIV G'CCGG_C
Cuts at: 269 690 3113 269
Size: 421 2423 2090
Fragments arranged by size:
2423 2090 421
NspI r_CATG'y
Cuts at: 1987 2487 3394 1987
Size: 500 907 3527
Fragments arranged by size:
3527 907 500
PciI A'CATG_T
Cuts at: 3390 3390
Size: 4934
PfoI T'CCnGG_A
Cuts at: 1520 1889 1520
Size: 369 4565
PmeI GTTT'AAAC
Cuts at: 1933 1933
Size: 4934
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4073 4105 4073
Size: 32 4902
PpuMI rG'GwC_Cy
Cuts at: 591 591
Size: 4934
PspOMI G'GGCC_C
Cuts at: 1589 1589
Size: 4934
PspXI vC'TCGA_Gb
Cuts at: 1897 1897
Size: 4934
PstI C_TGCA'G
Cuts at: 1418 1808 1981 2666 1418
Size: 390 173 685 3686
Fragments arranged by size:
3686 685 390 173
PvuI CG_AT'CG
Cuts at: 104 104
Size: 4934
PvuII CAG'CTG
Cuts at: 1099 1628 1809 2359 2719 1099
Size: 529 181 550 360 3314
Fragments arranged by size:
3314 550 529 360 181
RsrII CG'GwC_CG
Cuts at: 3129 3129
Size: 4934
SacI G_AGCT'C
Cuts at: 1898 1948 1898
Size: 50 4884
SalI G'TCGA_C
Cuts at: 1971 1971
Size: 4934
SapI GCTCTTCn'nnn_
Cuts at: 2957 3167 2957
Size: 210 4724
SbfI CC_TGCA'GG
Cuts at: 1981 1981
Size: 4934
ScaI AGT'ACT
Cuts at: 1720 4451 1720
Size: 2731 2203
SfcI C'TryA_G
Cuts at: 33 586 1414 1804 1816 1977 2662 3655 3846
Size: 553 828 390 12 161 685 993 191
Cuts at: 3846 33
Size: 1121
Fragments arranged by size:
1121 993 828 685 553 390 191 161
12
SfoI GGC'GCC
Cuts at: 450 2366 2614 450
Size: 1916 248 2770
Fragments arranged by size:
2770 1916 248
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 4934
SmaI CCC'GGG
Cuts at: 236 1588 1956 236
Size: 1352 368 3214
Fragments arranged by size:
3214 1352 368
SmlI C'TyrA_G
Cuts at: 1633 1897 2089 3004 3496 3758 1633
Size: 264 192 915 492 262 2809
Fragments arranged by size:
2809 915 492 264 262 192
SpeI A'CTAG_T
Cuts at: 67 67
Size: 4934
SphI G_CATG'C
Cuts at: 1987 2487 1987
Size: 500 4434
StuI AGG'CCT
Cuts at: 1399 1399
Size: 4934
StyI C'CwwG_G
Cuts at: 196 797 1029 1352 2068 2422 196
Size: 601 232 323 716 354 2708
Fragments arranged by size:
2708 716 601 354 323 232
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 153 238 583 826 2517 2650 3109 3318 153
Size: 85 345 243 1691 133 459 209 1769
Fragments arranged by size:
1769 1691 459 345 243 209 133 85
TatI w'GTAC_w
Cuts at: 1475 1598 1718 4449 1475
Size: 123 120 2731 1960
Fragments arranged by size:
2731 1960 123 120
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 726 758 4451 4483 726
Size: 32 3693 32 1177
Fragments arranged by size:
3693 1177 32 32
Tth111I GACn'n_nGTC
Cuts at: 961 2731 961
Size: 1770 3164
XbaI T'CTAG_A
Cuts at: 1965 1965
Size: 4934
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2429 2429
Size: 4934
XhoI C'TCGA_G
Cuts at: 1897 1897
Size: 4934
XmaI C'CCGG_G
Cuts at: 234 1586 1954 234
Size: 1352 368 3214
Fragments arranged by size:
3214 1352 368
Enzymes that do cut:
AarI AccI Acc65I AclI AcuI AflIII AgeI AhdI
AleI AloI AlwNI ApaI ApaLI ApoI AseI AsiSI
AvaI BamHI BanI BanII BbeI BbsI BbvCI BcgI
BciVI BclI BglI BglII BlpI Bme1580I BmrI BplI
BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI
BsaXI BseRI BseYI BsgI BsiEI BsiHKAI Bsp1286I BspEI
BspHI BspMI BsrBI BsrDI BsrFI BssSI BstAPI BstBI
BstEII BstXI BstYI BtgI BtgZI BtsI DraI DrdI
EaeI EagI EarI EciI EcoICRI Eco57MI EcoO109I EcoRI
FalI FspI HaeII Hin4I HincII HpaI KasI KpnI
MluI MmeI MscI MslI MspA1I NaeI NarI NgoMIV
NspI PciI PfoI PmeI PpiI PpuMI PspOMI PspXI
PstI PvuI PvuII RsrII SacI SalI SapI SbfI
ScaI SfcI SfoI SgfI SmaI SmlI SpeI SphI
StuI StyI TaqII TatI TstI Tth111I XbaI XcmI
XhoI XmaI
Enzymes that do not cut:
AatII AfeI AflII AlfI AscI AvrII BaeI BfrBI
BmgBI BmtI BsiWI BsmI BsmBI BsrGI BssHII BstZ17I
Bsu36I ClaI CspCI DraIII EcoNI EcoRV FseI FspAI
HindIII MfeI NcoI NdeI NheI NotI NruI NsiI
PacI PasI PflMI PmlI PshAI PsiI PsrI SacII
SanDI SexAI SfiI SgrAI SnaBI SrfI SspI SwaI
XmnI ZraI