Restriction map for
pF1KE0034
(Circular) (Six-base) MAPSORT of: pF1KE0034.seq Check: 2743 from: 1 to: 4601
pF1KE0034 4601 bp
With 182 enzymes: SgfI *
April 24, 2009 13:09 ..
AccI GT'mk_AC
Cuts at: 308 1446 1639 308
Size: 1138 193 3270
Fragments arranged by size:
3270 1138 193
Acc65I G'GTAC_C
Cuts at: 1617 1617
Size: 4601
AclI AA'CG_TT
Cuts at: 4441 4441
Size: 4601
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 218 1009 2426 2858 3605 3873 218
Size: 791 1417 432 747 268 946
Fragments arranged by size:
1417 946 791 747 432 268
AflIII A'CryG_T
Cuts at: 1953 3057 4034 1953
Size: 1104 977 2520
Fragments arranged by size:
2520 1104 977
AgeI A'CCGG_T
Cuts at: 2989 2989
Size: 4601
AleI CACnn'nnGTG
Cuts at: 833 833
Size: 4601
AlwNI CAG_nnn'CTG
Cuts at: 1906 3473 1906
Size: 1567 3034
ApaLI G'TGCA_C
Cuts at: 3371 3371
Size: 4601
ApoI r'AATT_y
Cuts at: 285 442 873 1402 1605 3954 4210 285
Size: 157 431 529 203 2349 256 676
Fragments arranged by size:
2349 676 529 431 256 203 157
AseI AT'TA_AT
Cuts at: 20 20
Size: 4601
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 4601
AvaI C'yCGr_G
Cuts at: 657 1255 1621 657
Size: 598 366 3637
Fragments arranged by size:
3637 598 366
BamHI G'GATC_C
Cuts at: 1626 1626
Size: 4601
BanI G'GyrC_C
Cuts at: 1617 2031 2279 2314 1617
Size: 414 248 35 3904
Fragments arranged by size:
3904 414 248 35
BanII G_rGCy'C
Cuts at: 1433 1615 2645 1433
Size: 182 1030 3389
Fragments arranged by size:
3389 1030 182
BbeI G_GCGC'C
Cuts at: 2035 2283 2035
Size: 248 4353
BbsI GAAGACnn'nnnn_
Cuts at: 647 1055 647
Size: 408 4193
BciVI GTATCCnnnnn_n'
Cuts at: 2499 3260 4601 2499
Size: 761 1341 2499
Fragments arranged by size:
2499 1341 761
BclI T'GATC_A
Cuts at: 2121 2121
Size: 4601
BfrBI ATG'CAT
Cuts at: 543 543
Size: 4601
BglI GCCn_nnn'nGGC
Cuts at: 582 582
Size: 4601
BglII A'GATC_T
Cuts at: 462 2116 4153 462
Size: 1654 2037 910
Fragments arranged by size:
2037 1654 910
BlpI GC'TnA_GC
Cuts at: 469 861 1322 1713 469
Size: 392 461 391 3357
Fragments arranged by size:
3357 461 392 391
Bme1580I G_kGCm'C
Cuts at: 2226 2319 3375 2226
Size: 93 1056 3452
Fragments arranged by size:
3452 1056 93
BmrI ACTGGGnnnn_n'
Cuts at: 1813 1894 2229 4373 1813
Size: 81 335 2144 2041
Fragments arranged by size:
2144 2041 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2968 2968
Size: 4601
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 307 1282 307
Size: 975 3626
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 234 1777 2656 3148 3446 234
Size: 1543 879 492 298 1389
Fragments arranged by size:
1543 1389 879 492 298
BsaI GGTCTCn'nnnn_
Cuts at: 117 1544 4305 117
Size: 1427 2761 413
Fragments arranged by size:
2761 1427 413
BsaAI yAC'GTr
Cuts at: 1954 1954
Size: 4601
BsaBI GATnn'nnATC
Cuts at: 6 974 2115 4152 6
Size: 968 1141 2037 455
Fragments arranged by size:
2037 1141 968 455
BsaHI Gr'CG_yC
Cuts at: 2032 2280 2982 4478 2032
Size: 248 702 1496 2155
Fragments arranged by size:
2155 1496 702 248
BsaWI w'CCGG_w
Cuts at: 424 1790 2311 2989 3263 3410 3821 424
Size: 1366 521 678 274 147 411 1204
Fragments arranged by size:
1366 1204 678 521 411 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 126 156 126
Size: 30 4571
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 147 825 1044 147
Size: 678 219 3704
Fragments arranged by size:
3704 678 219
BseYI C'CCAG_C
Cuts at: 548 815 1432 2026 2784 3361 3857 548
Size: 267 617 594 758 577 496 1292
Fragments arranged by size:
1292 758 617 594 577 496 267
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 959 959
Size: 4601
BsiEI CG_ry'CG
Cuts at: 104 2143 2189 3397 104
Size: 2039 46 1208 1308
Fragments arranged by size:
2039 1308 1208 46
BsiHKAI G_wGCw'C
Cuts at: 1615 2393 2583 3375 1615
Size: 778 190 792 2841
Fragments arranged by size:
2841 792 778 190
Bsp1286I G_dGCh'C
Cuts at: 1433 1615 2226 2319 2393 2583 2645 3375 1433
Size: 182 611 93 74 190 62 730 2659
Fragments arranged by size:
2659 730 611 190 182 93 74 62
BspEI T'CCGG_A
Cuts at: 424 3821 424
Size: 3397 1204
BspHI T'CATG_A
Cuts at: 3777 3777
Size: 4601
BspMI ACCTGCnnnn'nnnn_
Cuts at: 1651 1865 2167 2548 1651
Size: 214 302 381 3704
Fragments arranged by size:
3704 381 302 214
BsrBI CCG'CTC
Cuts at: 50 2893 2947 4431 4596 50
Size: 2843 54 1484 165 55
Fragments arranged by size:
2843 1484 165 55 54
BsrDI GCAATG_nn'
Cuts at: 2513 2513
Size: 4601
BsrFI r'CCGG_y
Cuts at: 2599 2780 2989 2599
Size: 181 209 4211
Fragments arranged by size:
4211 209 181
BsrGI T'GTAC_A
Cuts at: 904 904
Size: 4601
BssSI C'ACGA_G
Cuts at: 2872 3230 2872
Size: 358 4243
BstAPI GCAn_nnn'nTGC
Cuts at: 208 1978 208
Size: 1770 2831
BstBI TT'CG_AA
Cuts at: 2962 2962
Size: 4601
BstXI CCAn_nnnn'nTGG
Cuts at: 835 835
Size: 4601
BstYI r'GATC_y
Cuts at: 1 462 1626 2093 2116 2451 2697 3709 3795
Size: 461 1164 467 23 335 246 1012 86
Cuts at: 3795 3807 4153 1
Size: 12 346 449
Fragments arranged by size:
1164 1012 467 461 449 346 335 246
86 23 12
BstZ17I GTA'TAC
Cuts at: 1447 1447
Size: 4601
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 807 2551 2732 2895 807
Size: 1744 181 163 2513
Fragments arranged by size:
2513 1744 181 163
BtsI GCAGTG_nn'
Cuts at: 947 1486 1844 947
Size: 539 358 3704
Fragments arranged by size:
3704 539 358
DraI TTT'AAA
Cuts at: 761 1600 761
Size: 839 3762
DraIII CAC_nnn'GTG
Cuts at: 1282 1282
Size: 4601
DrdI GACnn_nn'nnGTC
Cuts at: 2307 3165 2307
Size: 858 3743
EaeI y'GGCC_r
Cuts at: 402 837 2186 2360 2751 2778 4123 402
Size: 435 1349 174 391 27 1345 880
Fragments arranged by size:
1349 1345 880 435 391 174 27
EagI C'GGCC_G
Cuts at: 2186 2186
Size: 4601
EarI CTCTTCn'nnn_
Cuts at: 1142 1349 2624 2834 1142
Size: 207 1275 210 2909
Fragments arranged by size:
2909 1275 210 207
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 2809 3119 3265 4411 2809
Size: 310 146 1146 2999
Fragments arranged by size:
2999 1146 310 146
EcoICRI GAG'CTC
Cuts at: 1613 1613
Size: 4601
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 218 307 1009 1282 2426 2858 3605 3873 218
Size: 89 702 273 1144 432 747 268 946
Fragments arranged by size:
1144 946 747 702 432 273 268 89
EcoO109I rG'GnC_Cy
Cuts at: 630 1740 630
Size: 1110 3491
EcoRI G'AATT_C
Cuts at: 1605 1605
Size: 4601
EcoRV GAT'ATC
Cuts at: 798 798
Size: 4601
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2620 2620
Size: 4601
FspI TGC'GCA
Cuts at: 2382 2382
Size: 4601
HaeII r_GCGC'y
Cuts at: 2035 2283 3305 4284 2035
Size: 248 1022 979 2352
Fragments arranged by size:
2352 1022 979 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 126 158 757 789 2443 2473 2475 2505 126
Size: 32 599 32 1654 30 2 30 2222
Fragments arranged by size:
2222 1654 599 32 32 30 30 2
HincII GTy'rAC
Cuts at: 1640 1640
Size: 4601
HindIII A'AGCT_T
Cuts at: 525 1242 525
Size: 717 3884
KasI G'GCGC_C
Cuts at: 2031 2279 2031
Size: 248 4353
KpnI G_GTAC'C
Cuts at: 1621 1621
Size: 4601
MfeI C'AATT_G
Cuts at: 781 781
Size: 4601
MluI A'CGCG_T
Cuts at: 4034 4034
Size: 4601
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1297 3272 3456 1297
Size: 1975 184 2442
Fragments arranged by size:
2442 1975 184
MscI TGG'CCA
Cuts at: 404 839 2362 404
Size: 435 1523 2643
Fragments arranged by size:
2643 1523 435
MslI CAynn'nnrTG
Cuts at: 833 2590 2717 833
Size: 1757 127 2717
Fragments arranged by size:
2717 1757 127
MspA1I CmG'CkG
Cuts at: 833 843 1712 2026 2386 3399 3644 833
Size: 10 869 314 360 1013 245 1790
Fragments arranged by size:
1790 1013 869 360 314 245 10
NaeI GCC'GGC
Cuts at: 2782 2782
Size: 4601
NarI GG'CG_CC
Cuts at: 2032 2280 2032
Size: 248 4353
NgoMIV G'CCGG_C
Cuts at: 2780 2780
Size: 4601
NsiI A_TGCA'T
Cuts at: 545 545
Size: 4601
NspI r_CATG'y
Cuts at: 1654 2154 3061 1654
Size: 500 907 3194
Fragments arranged by size:
3194 907 500
PciI A'CATG_T
Cuts at: 3057 3057
Size: 4601
PflMI CCAn_nnn'nTGG
Cuts at: 593 593
Size: 4601
PmeI GTTT'AAAC
Cuts at: 1600 1600
Size: 4601
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3740 3772 3740
Size: 32 4569
PpuMI rG'GwC_Cy
Cuts at: 630 630
Size: 4601
PsiI TTA'TAA
Cuts at: 1154 1154
Size: 4601
PstI C_TGCA'G
Cuts at: 502 1648 2333 502
Size: 1146 685 2770
Fragments arranged by size:
2770 1146 685
PvuI CG_AT'CG
Cuts at: 104 104
Size: 4601
PvuII CAG'CTG
Cuts at: 843 2026 2386 843
Size: 1183 360 3058
Fragments arranged by size:
3058 1183 360
RsrII CG'GwC_CG
Cuts at: 2796 2796
Size: 4601
SacI G_AGCT'C
Cuts at: 1615 1615
Size: 4601
SalI G'TCGA_C
Cuts at: 1638 1638
Size: 4601
SapI GCTCTTCn'nnn_
Cuts at: 2624 2834 2624
Size: 210 4391
SbfI CC_TGCA'GG
Cuts at: 1648 1648
Size: 4601
ScaI AGT'ACT
Cuts at: 420 4118 420
Size: 3698 903
SfcI C'TryA_G
Cuts at: 33 498 1644 2329 3322 3513 33
Size: 465 1146 685 993 191 1121
Fragments arranged by size:
1146 1121 993 685 465 191
SfoI GGC'GCC
Cuts at: 2033 2281 2033
Size: 248 4353
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 4601
SmaI CCC'GGG
Cuts at: 1623 1623
Size: 4601
SmlI C'TyrA_G
Cuts at: 213 657 1756 2671 3163 3425 213
Size: 444 1099 915 492 262 1389
Fragments arranged by size:
1389 1099 915 492 444 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 4601
SphI G_CATG'C
Cuts at: 1654 2154 1654
Size: 500 4101
SspI AAT'ATT
Cuts at: 1129 1269 1129
Size: 140 4461
StuI AGG'CCT
Cuts at: 219 219
Size: 4601
StyI C'CwwG_G
Cuts at: 1564 1735 2089 1564
Size: 171 354 4076
Fragments arranged by size:
4076 354 171
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 2184 2317 2776 2985 2184
Size: 133 459 209 3800
Fragments arranged by size:
3800 459 209 133
TatI w'GTAC_w
Cuts at: 234 418 904 4116 234
Size: 184 486 3212 719
Fragments arranged by size:
3212 719 486 184
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 4118 4150 4118
Size: 32 4569
Tth111I GACn'n_nGTC
Cuts at: 2398 2398
Size: 4601
XbaI T'CTAG_A
Cuts at: 144 1476 1632 144
Size: 1332 156 3113
Fragments arranged by size:
3113 1332 156
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2096 2096
Size: 4601
XhoI C'TCGA_G
Cuts at: 657 657
Size: 4601
XmaI C'CCGG_G
Cuts at: 1621 1621
Size: 4601
Enzymes that do cut:
AccI Acc65I AclI AcuI AflIII AgeI AleI AlwNI
ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII
BbeI BbsI BciVI BclI BfrBI BglI BglII BlpI
Bme1580I BmrI BplI BpmI BpuEI BsaI BsaAI BsaBI
BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI
Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI
BssSI BstAPI BstBI BstXI BstYI BstZ17I BtgZI BtsI
DraI DraIII DrdI EaeI EagI EarI EciI EcoICRI
Eco57MI EcoO109I EcoRI EcoRV FalI FspI HaeII Hin4I
HincII HindIII KasI KpnI MfeI MluI MmeI MscI
MslI MspA1I NaeI NarI NgoMIV NsiI NspI PciI
PflMI PmeI PpiI PpuMI PsiI PstI PvuI PvuII
RsrII SacI SalI SapI SbfI ScaI SfcI SfoI
SgfI SmaI SmlI SpeI SphI SspI StuI StyI
TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI
Enzymes that do not cut:
AarI AatII AfeI AflII AhdI AlfI AloI ApaI
AscI AvrII BaeI BbvCI BcgI BmgBI BmtI Bpu10I
BsiWI BsmI BsmBI BssHII BstEII Bsu36I BtgI ClaI
CspCI EcoNI FseI FspAI HpaI NcoI NdeI NheI
NotI NruI PacI PasI PfoI PmlI PshAI PspOMI
PspXI PsrI SacII SanDI SexAI SfiI SgrAI SnaBI
SrfI SwaI XmnI ZraI