Restriction map for
pF1KE0037
(Circular) (Six-base) MAPSORT of: pF1KE0037.seq Check: 5985 from: 1 to: 5333
pF1KE0037 5333 bp
With 182 enzymes: SgfI *
April 24, 2009 13:09 ..
AccI GT'mk_AC
Cuts at: 1118 2371 1118
Size: 1253 4080
Acc65I G'GTAC_C
Cuts at: 818 2349 818
Size: 1531 3802
AclI AA'CG_TT
Cuts at: 5173 5173
Size: 5333
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1318 1700 1776 2258 3158 3590 4337 4605 1318
Size: 382 76 482 900 432 747 268 2046
Fragments arranged by size:
2046 900 747 482 432 382 268 76
AfeI AGC'GCT
Cuts at: 364 364
Size: 5333
AflIII A'CryG_T
Cuts at: 2685 3789 4766 2685
Size: 1104 977 3252
Fragments arranged by size:
3252 1104 977
AgeI A'CCGG_T
Cuts at: 3721 3721
Size: 5333
AhdI GACnn_n'nnGTC
Cuts at: 378 378
Size: 5333
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 281 1059 1921 281
Size: 778 862 3693
Fragments arranged by size:
3693 862 778
AlwNI CAG_nnn'CTG
Cuts at: 225 354 763 1540 2002 2230 2638 4205 225
Size: 129 409 777 462 228 408 1567 1353
Fragments arranged by size:
1567 1353 777 462 409 408 228 129
ApaI G_GGCC'C
Cuts at: 472 1935 472
Size: 1463 3870
ApaLI G'TGCA_C
Cuts at: 1225 4103 1225
Size: 2878 2455
ApoI r'AATT_y
Cuts at: 1381 2337 4686 4942 1381
Size: 956 2349 256 1772
Fragments arranged by size:
2349 1772 956 256
AseI AT'TA_AT
Cuts at: 20 20
Size: 5333
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 5333
AvaI C'yCGr_G
Cuts at: 329 1785 2187 2353 329
Size: 1456 402 166 3309
Fragments arranged by size:
3309 1456 402 166
BamHI G'GATC_C
Cuts at: 275 1568 2358 275
Size: 1293 790 3250
Fragments arranged by size:
3250 1293 790
BanI G'GyrC_C
Cuts at: 664 818 1252 1306 2157 2349 2763 3011 3046
Size: 154 434 54 851 192 414 248 35
Cuts at: 3046 664
Size: 2951
Fragments arranged by size:
2951 851 434 414 248 192 154 54
35
BanII G_rGCy'C
Cuts at: 472 790 1274 1367 1935 2173 2347 3377 472
Size: 318 484 93 568 238 174 1030 2428
Fragments arranged by size:
2428 1030 568 484 318 238 174 93
BbeI G_GCGC'C
Cuts at: 2767 3015 2767
Size: 248 5085
BbsI GAAGACnn'nnnn_
Cuts at: 629 1497 629
Size: 868 4465
BbvCI CC'TCA_GC
Cuts at: 1135 1352 2249 1135
Size: 217 897 4219
Fragments arranged by size:
4219 897 217
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 90 124 1311 1345 90
Size: 34 1187 34 4078
Fragments arranged by size:
4078 1187 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3231 3992 5333 3231
Size: 761 1341 3231
Fragments arranged by size:
3231 1341 761
BclI T'GATC_A
Cuts at: 2853 2853
Size: 5333
BglII A'GATC_T
Cuts at: 1923 2848 4885 1923
Size: 925 2037 2371
Fragments arranged by size:
2371 2037 925
BlpI GC'TnA_GC
Cuts at: 784 2266 2445 784
Size: 1482 179 3672
Fragments arranged by size:
3672 1482 179
Bme1580I G_kGCm'C
Cuts at: 472 1229 1309 1935 2160 2958 3051 4107 472
Size: 757 80 626 225 798 93 1056 1698
Fragments arranged by size:
1698 1056 798 757 626 225 93 80
BmrI ACTGGGnnnn_n'
Cuts at: 485 1048 2545 2626 2961 5105 485
Size: 563 1497 81 335 2144 713
Fragments arranged by size:
2144 1497 713 563 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 864 1006 1545 3700 864
Size: 142 539 2155 2497
Fragments arranged by size:
2497 2155 539 142
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 253 262 285 306 1150 1288 1960 2193 2308
Size: 9 23 21 844 138 672 233 115
Cuts at: 2308 253
Size: 3278
Fragments arranged by size:
3278 844 672 233 138 115 23 21
9
Bpu10I CC'TnA_GC
Cuts at: 1135 1352 1549 2249 1135
Size: 217 197 700 4219
Fragments arranged by size:
4219 700 217 197
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2509 3388 3880 4178 2509
Size: 879 492 298 3664
Fragments arranged by size:
3664 879 492 298
BsaI GGTCTCn'nnnn_
Cuts at: 238 977 5037 238
Size: 739 4060 534
Fragments arranged by size:
4060 739 534
BsaAI yAC'GTr
Cuts at: 410 1194 2686 410
Size: 784 1492 3057
Fragments arranged by size:
3057 1492 784
BsaBI GATnn'nnATC
Cuts at: 6 2847 4884 6
Size: 2841 2037 455
Fragments arranged by size:
2841 2037 455
BsaHI Gr'CG_yC
Cuts at: 1314 1483 2764 3012 3714 5210 1314
Size: 169 1281 248 702 1496 1437
Fragments arranged by size:
1496 1437 1281 702 248 169
BsaWI w'CCGG_w
Cuts at: 500 934 1571 1703 2063 2522 3043 3721 3995
Size: 434 637 132 360 459 521 678 274
Cuts at: 3995 4142 4553 500
Size: 147 411 1280
Fragments arranged by size:
1280 678 637 521 459 434 411 360
274 147 132
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 304 334 575 605 304
Size: 30 241 30 5032
Fragments arranged by size:
5032 241 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 523 583 1369 1665 523
Size: 60 786 296 4191
Fragments arranged by size:
4191 786 296 60
BseYI C'CCAG_C
Cuts at: 132 138 162 739 1017 1089 1220 1243 1343
Size: 6 24 577 278 72 131 23 100
Cuts at: 1343 1612 1916 2758 3516 4093 4589 132
Size: 269 304 842 758 577 496 876
Fragments arranged by size:
876 842 758 577 577 496 304 278
269 131 100 72 24 23 6
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 367 1495 367
Size: 1128 4205
BsiEI CG_ry'CG
Cuts at: 104 398 2875 2921 4129 104
Size: 294 2477 46 1208 1308
Fragments arranged by size:
2477 1308 1208 294 46
BsiHKAI G_wGCw'C
Cuts at: 790 1038 1229 1274 2153 2347 3125 3315 4107
Size: 248 191 45 879 194 778 190 792
Cuts at: 4107 790
Size: 2016
Fragments arranged by size:
2016 879 792 778 248 194 191 190
45
BsiWI C'GTAC_G
Cuts at: 410 410
Size: 5333
Bsp1286I G_dGCh'C
Cuts at: 472 790 1038 1229 1274 1309 1367 1935 2153
Size: 318 248 191 45 35 58 568 218
Cuts at: 2153 2160 2173 2347 2958 3051 3125 3315 3377
Size: 7 13 174 611 93 74 190 62
Cuts at: 3377 4107 472
Size: 730 1698
Fragments arranged by size:
1698 730 611 568 318 248 218 191
190 174 93 74 62 58 45 35
13 7
BspEI T'CCGG_A
Cuts at: 934 2063 4553 934
Size: 1129 2490 1714
Fragments arranged by size:
2490 1714 1129
BspHI T'CATG_A
Cuts at: 4509 4509
Size: 5333
BspMI ACCTGCnnnn'nnnn_
Cuts at: 830 995 2049 2383 2597 2899 3280 830
Size: 165 1054 334 214 302 381 2883
Fragments arranged by size:
2883 1054 381 334 302 214 165
BsrBI CCG'CTC
Cuts at: 50 1529 3625 3679 5163 5328 50
Size: 1479 2096 54 1484 165 55
Fragments arranged by size:
2096 1484 1479 165 55 54
BsrDI GCAATG_nn'
Cuts at: 668 1685 3245 668
Size: 1017 1560 2756
Fragments arranged by size:
2756 1560 1017
BsrFI r'CCGG_y
Cuts at: 3331 3512 3721 3331
Size: 181 209 4943
Fragments arranged by size:
4943 209 181
BsrGI T'GTAC_A
Cuts at: 752 752
Size: 5333
BssSI C'ACGA_G
Cuts at: 3604 3962 3604
Size: 358 4975
BstAPI GCAn_nnn'nTGC
Cuts at: 354 2002 2710 354
Size: 1648 708 2977
Fragments arranged by size:
2977 1648 708
BstBI TT'CG_AA
Cuts at: 3694 3694
Size: 5333
BstXI CCAn_nnnn'nTGG
Cuts at: 210 456 1025 1155 210
Size: 246 569 130 4388
Fragments arranged by size:
4388 569 246 130
BstYI r'GATC_y
Cuts at: 1 236 275 1523 1568 1724 1923 2233 2358
Size: 235 39 1248 45 156 199 310 125
Cuts at: 2358 2825 2848 3183 3429 4441 4527 4539 4885
Size: 467 23 335 246 1012 86 12 346
Cuts at: 4885 1
Size: 449
Fragments arranged by size:
1248 1012 467 449 346 335 310 246
235 199 156 125 86 45 39 23
12
Bsu36I CC'TnA_GG
Cuts at: 831 1468 2308 831
Size: 637 840 3856
Fragments arranged by size:
3856 840 637
BtgI C'CryG_G
Cuts at: 1167 1376 1679 1167
Size: 209 303 4821
Fragments arranged by size:
4821 303 209
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 3283 3464 3627 3283
Size: 181 163 4989
Fragments arranged by size:
4989 181 163
BtsI GCAGTG_nn'
Cuts at: 118 1254 1985 2029 2576 118
Size: 1136 731 44 547 2875
Fragments arranged by size:
2875 1136 731 547 44
DraI TTT'AAA
Cuts at: 2332 2332
Size: 5333
DrdI GACnn_nn'nnGTC
Cuts at: 3039 3897 3039
Size: 858 4475
EaeI y'GGCC_r
Cuts at: 177 395 2918 3092 3483 3510 4855 177
Size: 218 2523 174 391 27 1345 655
Fragments arranged by size:
2523 1345 655 391 218 174 27
EagI C'GGCC_G
Cuts at: 395 2918 395
Size: 2523 2810
EarI CTCTTCn'nnn_
Cuts at: 909 1149 1385 3356 3566 909
Size: 240 236 1971 210 2676
Fragments arranged by size:
2676 1971 240 236 210
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 3541 3851 3997 5143 3541
Size: 310 146 1146 3731
Fragments arranged by size:
3731 1146 310 146
EcoICRI GAG'CTC
Cuts at: 788 1272 2345 788
Size: 484 1073 3776
Fragments arranged by size:
3776 1073 484
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 253 262 285 306 1150 1288 1318 1700 1776
Size: 9 23 21 844 138 30 382 76
Cuts at: 1776 1960 2193 2258 2308 3158 3590 4337 4605
Size: 184 233 65 50 850 432 747 268
Cuts at: 4605 253
Size: 981
Fragments arranged by size:
981 850 844 747 432 382 268 233
184 138 76 65 50 30 23 21
9
EcoNI CCTnn'n_nnAGG
Cuts at: 2208 2208
Size: 5333
EcoO109I rG'GnC_Cy
Cuts at: 222 339 372 469 588 870 1060 1289 1537
Size: 117 33 97 119 282 190 229 248
Cuts at: 1537 1931 1932 1963 2472 222
Size: 394 1 31 509 3083
Fragments arranged by size:
3083 509 394 282 248 229 190 119
117 97 33 31 1
EcoRI G'AATT_C
Cuts at: 1381 2337 1381
Size: 956 4377
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 3352 3352
Size: 5333
FspI TGC'GCA
Cuts at: 3114 3114
Size: 5333
HaeII r_GCGC'y
Cuts at: 366 2767 3015 4037 5016 366
Size: 2401 248 1022 979 683
Fragments arranged by size:
2401 1022 979 683 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 364 396 3175 3205 3207 3237 364
Size: 32 2779 30 2 30 2460
Fragments arranged by size:
2779 2460 32 30 30 2
HincII GTy'rAC
Cuts at: 2372 2372
Size: 5333
HindIII A'AGCT_T
Cuts at: 2240 2240
Size: 5333
KasI G'GCGC_C
Cuts at: 2763 3011 2763
Size: 248 5085
KpnI G_GTAC'C
Cuts at: 822 2353 822
Size: 1531 3802
MluI A'CGCG_T
Cuts at: 4766 4766
Size: 5333
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1172 4004 4188 1172
Size: 2832 184 2317
Fragments arranged by size:
2832 2317 184
MscI TGG'CCA
Cuts at: 3094 3094
Size: 5333
MslI CAynn'nnrTG
Cuts at: 1435 1447 2275 3322 3449 1435
Size: 12 828 1047 127 3319
Fragments arranged by size:
3319 1047 828 127 12
MspA1I CmG'CkG
Cuts at: 261 351 1220 1822 2444 2758 3118 4131 4376
Size: 90 869 602 622 314 360 1013 245
Cuts at: 4376 261
Size: 1218
Fragments arranged by size:
1218 1013 869 622 602 360 314 245
90
NaeI GCC'GGC
Cuts at: 3514 3514
Size: 5333
NarI GG'CG_CC
Cuts at: 2764 3012 2764
Size: 248 5085
NcoI C'CATG_G
Cuts at: 1679 1679
Size: 5333
NgoMIV G'CCGG_C
Cuts at: 3512 3512
Size: 5333
NspI r_CATG'y
Cuts at: 2386 2886 3793 2386
Size: 500 907 3926
Fragments arranged by size:
3926 907 500
PciI A'CATG_T
Cuts at: 3789 3789
Size: 5333
PflMI CCAn_nnn'nTGG
Cuts at: 113 225 815 1540 113
Size: 112 590 725 3906
Fragments arranged by size:
3906 725 590 112
PfoI T'CCnGG_A
Cuts at: 239 278 971 1265 239
Size: 39 693 294 4307
Fragments arranged by size:
4307 693 294 39
PmeI GTTT'AAAC
Cuts at: 2332 2332
Size: 5333
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4472 4504 4472
Size: 32 5301
PpuMI rG'GwC_Cy
Cuts at: 222 339 372 870 1060 1289 222
Size: 117 33 498 190 229 4266
Fragments arranged by size:
4266 498 229 190 117 33
PspOMI G'GGCC_C
Cuts at: 468 1931 468
Size: 1463 3870
PstI C_TGCA'G
Cuts at: 362 1412 1910 1980 2007 2036 2060 2380 3065
Size: 1050 498 70 27 29 24 320 685
Cuts at: 3065 362
Size: 2630
Fragments arranged by size:
2630 1050 685 498 320 70 29 27
24
PvuI CG_AT'CG
Cuts at: 104 104
Size: 5333
PvuII CAG'CTG
Cuts at: 261 351 1220 2758 3118 261
Size: 90 869 1538 360 2476
Fragments arranged by size:
2476 1538 869 360 90
RsrII CG'GwC_CG
Cuts at: 3528 3528
Size: 5333
SacI G_AGCT'C
Cuts at: 790 1274 2347 790
Size: 484 1073 3776
Fragments arranged by size:
3776 1073 484
SalI G'TCGA_C
Cuts at: 2370 2370
Size: 5333
SanDI GG'GwC_CC
Cuts at: 870 1060 1289 870
Size: 190 229 4914
Fragments arranged by size:
4914 229 190
SapI GCTCTTCn'nnn_
Cuts at: 3356 3566 3356
Size: 210 5123
SbfI CC_TGCA'GG
Cuts at: 1910 2060 2380 1910
Size: 150 320 4863
Fragments arranged by size:
4863 320 150
ScaI AGT'ACT
Cuts at: 4850 4850
Size: 5333
SexAI A'CCwGG_T
Cuts at: 341 341
Size: 5333
SfcI C'TryA_G
Cuts at: 33 358 637 1050 1408 1881 1906 1976 2003
Size: 325 279 413 358 473 25 70 27
Cuts at: 2003 2032 2056 2139 2376 3061 4054 4245 33
Size: 29 24 83 237 685 993 191 1121
Fragments arranged by size:
1121 993 685 473 413 358 325 279
237 191 83 70 29 27 25 24
SfoI GGC'GCC
Cuts at: 2765 3013 2765
Size: 248 5085
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 5333
SmaI CCC'GGG
Cuts at: 1787 2189 2355 1787
Size: 402 166 4765
Fragments arranged by size:
4765 402 166
SmlI C'TyrA_G
Cuts at: 2488 3403 3895 4157 2488
Size: 915 492 262 3664
Fragments arranged by size:
3664 915 492 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 5333
SphI G_CATG'C
Cuts at: 2386 2886 2386
Size: 500 4833
StuI AGG'CCT
Cuts at: 386 403 2313 386
Size: 17 1910 3406
Fragments arranged by size:
3406 1910 17
StyI C'CwwG_G
Cuts at: 707 1679 2009 2467 2821 707
Size: 972 330 458 354 3219
Fragments arranged by size:
3219 972 458 354 330
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 1212 1618 2916 3049 3508 3717 1212
Size: 406 1298 133 459 209 2828
Fragments arranged by size:
2828 1298 459 406 209 133
TatI w'GTAC_w
Cuts at: 752 4848 752
Size: 4096 1237
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 304 336 575 607 1299 1331 4850 4882 304
Size: 32 239 32 692 32 3519 32 755
Fragments arranged by size:
3519 755 692 239 32 32 32 32
Tth111I GACn'n_nGTC
Cuts at: 3130 3130
Size: 5333
XbaI T'CTAG_A
Cuts at: 2364 2364
Size: 5333
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 1924 2828 1924
Size: 904 4429
XmaI C'CCGG_G
Cuts at: 1785 2187 2353 1785
Size: 402 166 4765
Fragments arranged by size:
4765 402 166
XmnI GAAnn'nnTTC
Cuts at: 803 803
Size: 5333
Enzymes that do cut:
AccI Acc65I AclI AcuI AfeI AflIII AgeI AhdI
AlfI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI
BamHI BanI BanII BbeI BbsI BbvCI BcgI BciVI
BclI BglII BlpI Bme1580I BmrI BplI BpmI Bpu10I
BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI
BseYI BsgI BsiEI BsiHKAI BsiWI Bsp1286I BspEI BspHI
BspMI BsrBI BsrDI BsrFI BsrGI BssSI BstAPI BstBI
BstXI BstYI Bsu36I BtgI BtgZI BtsI DraI DrdI
EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I
EcoRI FalI FspI HaeII Hin4I HincII HindIII KasI
KpnI MluI MmeI MscI MslI MspA1I NaeI NarI
NcoI NgoMIV NspI PciI PflMI PfoI PmeI PpiI
PpuMI PspOMI PstI PvuI PvuII RsrII SacI SalI
SanDI SapI SbfI ScaI SexAI SfcI SfoI SgfI
SmaI SmlI SpeI SphI StuI StyI TaqII TatI
TstI Tth111I XbaI XcmI XmaI XmnI
Enzymes that do not cut:
AarI AatII AflII AleI AloI AscI AvrII BaeI
BfrBI BglI BmgBI BmtI BsmI BsmBI BssHII BstEII
BstZ17I ClaI CspCI DraIII EcoRV FseI FspAI HpaI
MfeI NdeI NheI NotI NruI NsiI PacI PasI
PmlI PshAI PsiI PspXI PsrI SacII SfiI SgrAI
SnaBI SrfI SspI SwaI XhoI ZraI