Restriction map for
pF1KE0039
(Circular) (Six-base) MAPSORT of: pF1KE0039.seq Check: 1502 from: 1 to: 4652
pF1KE0039 4652 bp
With 182 enzymes: SgfI *
April 24, 2009 13:09 ..
AccI GT'mk_AC
Cuts at: 1690 1690
Size: 4652
Acc65I G'GTAC_C
Cuts at: 1668 1668
Size: 4652
AclI AA'CG_TT
Cuts at: 4492 4492
Size: 4652
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 2477 2909 3656 3924 2477
Size: 432 747 268 3205
Fragments arranged by size:
3205 747 432 268
AflIII A'CryG_T
Cuts at: 1221 2004 3108 4085 1221
Size: 783 1104 977 1788
Fragments arranged by size:
1788 1104 977 783
AgeI A'CCGG_T
Cuts at: 3040 3040
Size: 4652
AleI CACnn'nnGTG
Cuts at: 375 375
Size: 4652
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 1318 1350 1318
Size: 32 4620
AlwNI CAG_nnn'CTG
Cuts at: 331 1957 3524 331
Size: 1626 1567 1459
Fragments arranged by size:
1626 1567 1459
ApaLI G'TGCA_C
Cuts at: 3422 3422
Size: 4652
ApoI r'AATT_y
Cuts at: 293 541 757 1656 4005 4261 293
Size: 248 216 899 2349 256 684
Fragments arranged by size:
2349 899 684 256 248 216
AseI AT'TA_AT
Cuts at: 20 1057 20
Size: 1037 3615
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 4652
AvaI C'yCGr_G
Cuts at: 593 1672 593
Size: 1079 3573
BamHI G'GATC_C
Cuts at: 831 1677 831
Size: 846 3806
BanI G'GyrC_C
Cuts at: 384 1010 1668 2082 2330 2365 384
Size: 626 658 414 248 35 2671
Fragments arranged by size:
2671 658 626 414 248 35
BanII G_rGCy'C
Cuts at: 779 1666 2696 779
Size: 887 1030 2735
Fragments arranged by size:
2735 1030 887
BbeI G_GCGC'C
Cuts at: 2086 2334 2086
Size: 248 4404
BbsI GAAGACnn'nnnn_
Cuts at: 373 373
Size: 4652
BciVI GTATCCnnnnn_n'
Cuts at: 1384 2550 3311 4652 1384
Size: 1166 761 1341 1384
Fragments arranged by size:
1384 1341 1166 761
BclI T'GATC_A
Cuts at: 792 1157 2172 792
Size: 365 1015 3272
Fragments arranged by size:
3272 1015 365
BglI GCCn_nnn'nGGC
Cuts at: 404 404
Size: 4652
BglII A'GATC_T
Cuts at: 2167 4204 2167
Size: 2037 2615
BlpI GC'TnA_GC
Cuts at: 911 1764 911
Size: 853 3799
Bme1580I G_kGCm'C
Cuts at: 2277 2370 3426 2277
Size: 93 1056 3503
Fragments arranged by size:
3503 1056 93
BmgBI CAC'GTC
Cuts at: 1222 1222
Size: 4652
BmrI ACTGGGnnnn_n'
Cuts at: 781 1864 1945 2280 4424 781
Size: 1083 81 335 2144 1009
Fragments arranged by size:
2144 1083 1009 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3019 3019
Size: 4652
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1317 1317
Size: 4652
Bpu10I CC'TnA_GC
Cuts at: 206 206
Size: 4652
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 376 1828 2707 3199 3497 376
Size: 1452 879 492 298 1531
Fragments arranged by size:
1531 1452 879 492 298
BsaI GGTCTCn'nnnn_
Cuts at: 4356 4356
Size: 4652
BsaAI yAC'GTr
Cuts at: 2005 2005
Size: 4652
BsaBI GATnn'nnATC
Cuts at: 6 2166 4203 6
Size: 2160 2037 455
Fragments arranged by size:
2160 2037 455
BsaHI Gr'CG_yC
Cuts at: 2083 2331 3033 4529 2083
Size: 248 702 1496 2206
Fragments arranged by size:
2206 1496 702 248
BsaWI w'CCGG_w
Cuts at: 170 465 1841 2362 3040 3314 3461 3872 170
Size: 295 1376 521 678 274 147 411 950
Fragments arranged by size:
1376 950 678 521 411 295 274 147
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1509 1509
Size: 4652
BseYI C'CCAG_C
Cuts at: 2077 2835 3412 3908 2077
Size: 758 577 496 2821
Fragments arranged by size:
2821 758 577 496
BsiEI CG_ry'CG
Cuts at: 104 2194 2240 3448 104
Size: 2090 46 1208 1308
Fragments arranged by size:
2090 1308 1208 46
BsiHKAI G_wGCw'C
Cuts at: 1666 2444 2634 3426 1666
Size: 778 190 792 2892
Fragments arranged by size:
2892 792 778 190
Bsp1286I G_dGCh'C
Cuts at: 779 1666 2277 2370 2444 2634 2696 3426 779
Size: 887 611 93 74 190 62 730 2005
Fragments arranged by size:
2005 887 730 611 190 93 74 62
BspEI T'CCGG_A
Cuts at: 170 465 3872 170
Size: 295 3407 950
Fragments arranged by size:
3407 950 295
BspHI T'CATG_A
Cuts at: 362 575 753 1475 3828 362
Size: 213 178 722 2353 1186
Fragments arranged by size:
2353 1186 722 213 178
BspMI ACCTGCnnnn'nnnn_
Cuts at: 915 1702 1916 2218 2599 915
Size: 787 214 302 381 2968
Fragments arranged by size:
2968 787 381 302 214
BsrBI CCG'CTC
Cuts at: 50 2944 2998 4482 4647 50
Size: 2894 54 1484 165 55
Fragments arranged by size:
2894 1484 165 55 54
BsrDI GCAATG_nn'
Cuts at: 2564 2564
Size: 4652
BsrFI r'CCGG_y
Cuts at: 2650 2831 3040 2650
Size: 181 209 4262
Fragments arranged by size:
4262 209 181
BssSI C'ACGA_G
Cuts at: 476 2923 3281 476
Size: 2447 358 1847
Fragments arranged by size:
2447 1847 358
BstAPI GCAn_nnn'nTGC
Cuts at: 481 1292 2029 481
Size: 811 737 3104
Fragments arranged by size:
3104 811 737
BstBI TT'CG_AA
Cuts at: 3013 3013
Size: 4652
BstYI r'GATC_y
Cuts at: 1 831 1022 1677 2144 2167 2502 2748 3760
Size: 830 191 655 467 23 335 246 1012
Cuts at: 3760 3846 3858 4204 1
Size: 86 12 346 449
Fragments arranged by size:
1012 830 655 467 449 346 335 246
191 86 23 12
Bsu36I CC'TnA_GG
Cuts at: 899 899
Size: 4652
BtgI C'CryG_G
Cuts at: 600 1322 1422 600
Size: 722 100 3830
Fragments arranged by size:
3830 722 100
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 2602 2783 2946 2602
Size: 181 163 4308
Fragments arranged by size:
4308 181 163
BtsI GCAGTG_nn'
Cuts at: 1895 1895
Size: 4652
DraI TTT'AAA
Cuts at: 1002 1137 1651 1002
Size: 135 514 4003
Fragments arranged by size:
4003 514 135
DrdI GACnn_nn'nnGTC
Cuts at: 2358 3216 2358
Size: 858 3794
EaeI y'GGCC_r
Cuts at: 645 1072 2237 2411 2802 2829 4174 645
Size: 427 1165 174 391 27 1345 1123
Fragments arranged by size:
1345 1165 1123 427 391 174 27
EagI C'GGCC_G
Cuts at: 2237 2237
Size: 4652
EarI CTCTTCn'nnn_
Cuts at: 2675 2885 2675
Size: 210 4442
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 2860 3170 3316 4462 2860
Size: 310 146 1146 3050
Fragments arranged by size:
3050 1146 310 146
EcoICRI GAG'CTC
Cuts at: 1664 1664
Size: 4652
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1317 2477 2909 3656 3924 1317
Size: 1160 432 747 268 2045
Fragments arranged by size:
2045 1160 747 432 268
EcoO109I rG'GnC_Cy
Cuts at: 1791 1791
Size: 4652
EcoRI G'AATT_C
Cuts at: 1656 1656
Size: 4652
EcoRV GAT'ATC
Cuts at: 864 1035 1536 864
Size: 171 501 3980
Fragments arranged by size:
3980 501 171
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2671 2671
Size: 4652
FspI TGC'GCA
Cuts at: 2433 2433
Size: 4652
HaeII r_GCGC'y
Cuts at: 117 2086 2334 3356 4335 117
Size: 1969 248 1022 979 434
Fragments arranged by size:
1969 1022 979 434 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1157 1189 1563 1595 2494 2524 2526 2556 1157
Size: 32 374 32 899 30 2 30 3253
Fragments arranged by size:
3253 899 374 32 32 30 30 2
HincII GTy'rAC
Cuts at: 1691 1691
Size: 4652
HindIII A'AGCT_T
Cuts at: 1593 1593
Size: 4652
KasI G'GCGC_C
Cuts at: 2082 2330 2082
Size: 248 4404
KpnI G_GTAC'C
Cuts at: 1672 1672
Size: 4652
MluI A'CGCG_T
Cuts at: 4085 4085
Size: 4652
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 3323 3507 3323
Size: 184 4468
MscI TGG'CCA
Cuts at: 647 1074 2413 647
Size: 427 1339 2886
Fragments arranged by size:
2886 1339 427
MslI CAynn'nnrTG
Cuts at: 375 692 788 941 2641 2768 375
Size: 317 96 153 1700 127 2259
Fragments arranged by size:
2259 1700 317 153 127 96
MspA1I CmG'CkG
Cuts at: 1087 1763 2077 2437 3450 3695 1087
Size: 676 314 360 1013 245 2044
Fragments arranged by size:
2044 1013 676 360 314 245
NaeI GCC'GGC
Cuts at: 2833 2833
Size: 4652
NarI GG'CG_CC
Cuts at: 2083 2331 2083
Size: 248 4404
NcoI C'CATG_G
Cuts at: 600 1322 600
Size: 722 3930
NgoMIV G'CCGG_C
Cuts at: 2831 2831
Size: 4652
NspI r_CATG'y
Cuts at: 1705 2205 3112 1705
Size: 500 907 3245
Fragments arranged by size:
3245 907 500
PasI CC'CwG_GG
Cuts at: 418 418
Size: 4652
PciI A'CATG_T
Cuts at: 3108 3108
Size: 4652
PflMI CCAn_nnn'nTGG
Cuts at: 1323 1323
Size: 4652
PfoI T'CCnGG_A
Cuts at: 1215 1362 1215
Size: 147 4505
PmeI GTTT'AAAC
Cuts at: 1651 1651
Size: 4652
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3791 3823 3791
Size: 32 4620
PshAI GACnn'nnGTC
Cuts at: 595 595
Size: 4652
PsiI TTA'TAA
Cuts at: 142 142
Size: 4652
PspXI vC'TCGA_Gb
Cuts at: 593 593
Size: 4652
PsrI GAACnnnnnnTACnnnnnnn_nnnnn'
Cuts at: 914 946 914
Size: 32 4620
PstI C_TGCA'G
Cuts at: 1699 2384 1699
Size: 685 3967
PvuI CG_AT'CG
Cuts at: 104 104
Size: 4652
PvuII CAG'CTG
Cuts at: 1087 2077 2437 1087
Size: 990 360 3302
Fragments arranged by size:
3302 990 360
RsrII CG'GwC_CG
Cuts at: 2847 2847
Size: 4652
SacI G_AGCT'C
Cuts at: 1666 1666
Size: 4652
SalI G'TCGA_C
Cuts at: 1689 1689
Size: 4652
SapI GCTCTTCn'nnn_
Cuts at: 2675 2885 2675
Size: 210 4442
SbfI CC_TGCA'GG
Cuts at: 1699 1699
Size: 4652
ScaI AGT'ACT
Cuts at: 4169 4169
Size: 4652
SfcI C'TryA_G
Cuts at: 33 444 1695 2380 3373 3564 33
Size: 411 1251 685 993 191 1121
Fragments arranged by size:
1251 1121 993 685 411 191
SfoI GGC'GCC
Cuts at: 2084 2332 2084
Size: 248 4404
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 4652
SmaI CCC'GGG
Cuts at: 1674 1674
Size: 4652
SmlI C'TyrA_G
Cuts at: 355 593 1807 2722 3214 3476 355
Size: 238 1214 915 492 262 1531
Fragments arranged by size:
1531 1214 915 492 262 238
SpeI A'CTAG_T
Cuts at: 67 67
Size: 4652
SphI G_CATG'C
Cuts at: 1705 2205 1705
Size: 500 4152
SspI AAT'ATT
Cuts at: 1464 1464
Size: 4652
StyI C'CwwG_G
Cuts at: 600 632 713 1322 1786 2140 600
Size: 32 81 609 464 354 3112
Fragments arranged by size:
3112 609 464 354 81 32
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 387 410 664 2235 2368 2827 3036 387
Size: 23 254 1571 133 459 209 2003
Fragments arranged by size:
2003 1571 459 254 209 133 23
TatI w'GTAC_w
Cuts at: 1397 4167 1397
Size: 2770 1882
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 311 343 4169 4201 311
Size: 32 3826 32 762
Fragments arranged by size:
3826 762 32 32
Tth111I GACn'n_nGTC
Cuts at: 2449 2449
Size: 4652
XbaI T'CTAG_A
Cuts at: 1683 1683
Size: 4652
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 883 2147 883
Size: 1264 3388
XhoI C'TCGA_G
Cuts at: 593 593
Size: 4652
XmaI C'CCGG_G
Cuts at: 1672 1672
Size: 4652
Enzymes that do cut:
AccI Acc65I AclI AcuI AflIII AgeI AleI AloI
AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI BanI
BanII BbeI BbsI BciVI BclI BglI BglII BlpI
Bme1580I BmgBI BmrI BplI BpmI Bpu10I BpuEI BsaI
BsaAI BsaBI BsaHI BsaWI BseRI BseYI BsiEI BsiHKAI
Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BssSI
BstAPI BstBI BstYI Bsu36I BtgI BtgZI BtsI DraI
DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoO109I
EcoRI EcoRV FalI FspI HaeII Hin4I HincII HindIII
KasI KpnI MluI MmeI MscI MslI MspA1I NaeI
NarI NcoI NgoMIV NspI PasI PciI PflMI PfoI
PmeI PpiI PshAI PsiI PspXI PsrI PstI PvuI
PvuII RsrII SacI SalI SapI SbfI ScaI SfcI
SfoI SgfI SmaI SmlI SpeI SphI SspI StyI
TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI
Enzymes that do not cut:
AarI AatII AfeI AflII AhdI AlfI ApaI AscI
AvrII BaeI BbvCI BcgI BfrBI BmtI BsaXI BsgI
BsiWI BsmI BsmBI BsrGI BssHII BstEII BstXI BstZ17I
ClaI CspCI DraIII EcoNI FseI FspAI HpaI MfeI
NdeI NheI NotI NruI NsiI PacI PmlI PpuMI
PspOMI SacII SanDI SexAI SfiI SgrAI SnaBI SrfI
StuI SwaI XmnI ZraI