Restriction map for
pF1KE0041
(Circular) (Six-base) MAPSORT of: pF1KE0041.seq Check: 6896 from: 1 to: 4673
pF1KE0041 4673 bp
With 182 enzymes: SgfI *
April 24, 2009 13:09 ..
AccI GT'mk_AC
Cuts at: 1711 1711
Size: 4673
Acc65I G'GTAC_C
Cuts at: 1689 1689
Size: 4673
AclI AA'CG_TT
Cuts at: 4513 4513
Size: 4673
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 337 1105 2498 2930 3677 3945 337
Size: 768 1393 432 747 268 1065
Fragments arranged by size:
1393 1065 768 747 432 268
AflIII A'CryG_T
Cuts at: 1292 1321 2025 3129 4106 1292
Size: 29 704 1104 977 1859
Fragments arranged by size:
1859 1104 977 704 29
AgeI A'CCGG_T
Cuts at: 3061 3061
Size: 4673
AleI CACnn'nnGTG
Cuts at: 1402 1480 1402
Size: 78 4595
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 155 187 155
Size: 32 4641
AlwNI CAG_nnn'CTG
Cuts at: 243 1978 3545 243
Size: 1735 1567 1371
Fragments arranged by size:
1735 1567 1371
ApaLI G'TGCA_C
Cuts at: 3443 3443
Size: 4673
ApoI r'AATT_y
Cuts at: 156 506 850 1141 1444 1677 4026 4282 156
Size: 350 344 291 303 233 2349 256 547
Fragments arranged by size:
2349 547 350 344 303 291 256 233
AseI AT'TA_AT
Cuts at: 20 20
Size: 4673
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 4673
AvaI C'yCGr_G
Cuts at: 371 1693 371
Size: 1322 3351
BamHI G'GATC_C
Cuts at: 1698 1698
Size: 4673
BanI G'GyrC_C
Cuts at: 260 1689 2103 2351 2386 260
Size: 1429 414 248 35 2547
Fragments arranged by size:
2547 1429 414 248 35
BanII G_rGCy'C
Cuts at: 780 1687 2717 780
Size: 907 1030 2736
Fragments arranged by size:
2736 1030 907
BbeI G_GCGC'C
Cuts at: 2107 2355 2107
Size: 248 4425
BbsI GAAGACnn'nnnn_
Cuts at: 813 1295 1560 813
Size: 482 265 3926
Fragments arranged by size:
3926 482 265
BciVI GTATCCnnnnn_n'
Cuts at: 2571 3332 4673 2571
Size: 761 1341 2571
Fragments arranged by size:
2571 1341 761
BclI T'GATC_A
Cuts at: 2193 2193
Size: 4673
BfrBI ATG'CAT
Cuts at: 581 581
Size: 4673
BglII A'GATC_T
Cuts at: 2188 4225 2188
Size: 2037 2636
BlpI GC'TnA_GC
Cuts at: 1785 1785
Size: 4673
Bme1580I G_kGCm'C
Cuts at: 263 604 1123 2298 2391 3447 263
Size: 341 519 1175 93 1056 1489
Fragments arranged by size:
1489 1175 1056 519 341 93
BmgBI CAC'GTC
Cuts at: 705 705
Size: 4673
BmrI ACTGGGnnnn_n'
Cuts at: 1885 1966 2301 4445 1885
Size: 81 335 2144 2113
Fragments arranged by size:
2144 2113 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3040 3040
Size: 4673
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 381 625 1032 381
Size: 244 407 4022
Fragments arranged by size:
4022 407 244
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 293 1849 2728 3220 3518 293
Size: 1556 879 492 298 1448
Fragments arranged by size:
1556 1448 879 492 298
BsaI GGTCTCn'nnnn_
Cuts at: 4377 4377
Size: 4673
BsaAI yAC'GTr
Cuts at: 993 2026 993
Size: 1033 3640
BsaBI GATnn'nnATC
Cuts at: 6 625 2187 4224 6
Size: 619 1562 2037 455
Fragments arranged by size:
2037 1562 619 455
BsaHI Gr'CG_yC
Cuts at: 2104 2352 3054 4550 2104
Size: 248 702 1496 2227
Fragments arranged by size:
2227 1496 702 248
BsaWI w'CCGG_w
Cuts at: 1862 2383 3061 3335 3482 3893 1862
Size: 521 678 274 147 411 2642
Fragments arranged by size:
2642 678 521 411 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 155 185 155
Size: 30 4643
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1321 1321
Size: 4673
BseYI C'CCAG_C
Cuts at: 682 689 1357 2098 2856 3433 3929 682
Size: 7 668 741 758 577 496 1426
Fragments arranged by size:
1426 758 741 668 577 496 7
BsiEI CG_ry'CG
Cuts at: 104 2215 2261 3469 104
Size: 2111 46 1208 1308
Fragments arranged by size:
2111 1308 1208 46
BsiHKAI G_wGCw'C
Cuts at: 1687 2465 2655 3447 1687
Size: 778 190 792 2913
Fragments arranged by size:
2913 792 778 190
Bsp1286I G_dGCh'C
Cuts at: 263 604 780 1123 1687 2298 2391 2465 2655
Size: 341 176 343 564 611 93 74 190
Cuts at: 2655 2717 3447 263
Size: 62 730 1489
Fragments arranged by size:
1489 730 611 564 343 341 190 176
93 74 62
BspEI T'CCGG_A
Cuts at: 3893 3893
Size: 4673
BspHI T'CATG_A
Cuts at: 1373 3849 1373
Size: 2476 2197
BspMI ACCTGCnnnn'nnnn_
Cuts at: 120 1723 1937 2239 2620 120
Size: 1603 214 302 381 2173
Fragments arranged by size:
2173 1603 381 302 214
BsrBI CCG'CTC
Cuts at: 50 208 2965 3019 4503 4668 50
Size: 158 2757 54 1484 165 55
Fragments arranged by size:
2757 1484 165 158 55 54
BsrDI GCAATG_nn'
Cuts at: 2585 2585
Size: 4673
BsrFI r'CCGG_y
Cuts at: 2671 2852 3061 2671
Size: 181 209 4283
Fragments arranged by size:
4283 209 181
BssSI C'ACGA_G
Cuts at: 2944 3302 2944
Size: 358 4315
BstAPI GCAn_nnn'nTGC
Cuts at: 2050 2050
Size: 4673
BstBI TT'CG_AA
Cuts at: 804 3034 804
Size: 2230 2443
BstYI r'GATC_y
Cuts at: 1 1698 2165 2188 2523 2769 3781 3867 3879
Size: 1697 467 23 335 246 1012 86 12
Cuts at: 3879 4225 1
Size: 346 449
Fragments arranged by size:
1697 1012 467 449 346 335 246 86
23 12
BtgI C'CryG_G
Cuts at: 107 107
Size: 4673
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 2623 2804 2967 2623
Size: 181 163 4329
Fragments arranged by size:
4329 181 163
BtsI GCAGTG_nn'
Cuts at: 1916 1916
Size: 4673
DraI TTT'AAA
Cuts at: 795 1672 795
Size: 877 3796
DrdI GACnn_nn'nnGTC
Cuts at: 2379 3237 2379
Size: 858 3815
EaeI y'GGCC_r
Cuts at: 2258 2432 2823 2850 4195 2258
Size: 174 391 27 1345 2736
Fragments arranged by size:
2736 1345 391 174 27
EagI C'GGCC_G
Cuts at: 2258 2258
Size: 4673
EarI CTCTTCn'nnn_
Cuts at: 215 383 858 2696 2906 215
Size: 168 475 1838 210 1982
Fragments arranged by size:
1982 1838 475 210 168
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 2881 3191 3337 4483 2881
Size: 310 146 1146 3071
Fragments arranged by size:
3071 1146 310 146
EcoICRI GAG'CTC
Cuts at: 1685 1685
Size: 4673
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 337 381 625 1032 1105 2498 2930 3677 3945
Size: 44 244 407 73 1393 432 747 268
Cuts at: 3945 337
Size: 1065
Fragments arranged by size:
1393 1065 747 432 407 268 244 73
44
EcoNI CCTnn'n_nnAGG
Cuts at: 721 742 721
Size: 21 4652
EcoO109I rG'GnC_Cy
Cuts at: 1812 1812
Size: 4673
EcoRI G'AATT_C
Cuts at: 1141 1677 1141
Size: 536 4137
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2692 2692
Size: 4673
FspI TGC'GCA
Cuts at: 2454 2454
Size: 4673
HaeII r_GCGC'y
Cuts at: 2107 2355 3377 4356 2107
Size: 248 1022 979 2424
Fragments arranged by size:
2424 1022 979 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 376 408 645 677 1530 1562 2515 2545 2547
Size: 32 237 32 853 32 953 30 2
Cuts at: 2547 2577 376
Size: 30 2472
Fragments arranged by size:
2472 953 853 237 32 32 32 30
30 2
HincII GTy'rAC
Cuts at: 1712 1712
Size: 4673
KasI G'GCGC_C
Cuts at: 2103 2351 2103
Size: 248 4425
KpnI G_GTAC'C
Cuts at: 1693 1693
Size: 4673
MluI A'CGCG_T
Cuts at: 4106 4106
Size: 4673
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1170 1388 3344 3528 1170
Size: 218 1956 184 2315
Fragments arranged by size:
2315 1956 218 184
MscI TGG'CCA
Cuts at: 2434 2434
Size: 4673
MslI CAynn'nnrTG
Cuts at: 531 1309 1402 1480 2662 2789 531
Size: 778 93 78 1182 127 2415
Fragments arranged by size:
2415 1182 778 127 93 78
MspA1I CmG'CkG
Cuts at: 442 1784 2098 2458 3471 3716 442
Size: 1342 314 360 1013 245 1399
Fragments arranged by size:
1399 1342 1013 360 314 245
NaeI GCC'GGC
Cuts at: 2854 2854
Size: 4673
NarI GG'CG_CC
Cuts at: 2104 2352 2104
Size: 248 4425
NcoI C'CATG_G
Cuts at: 107 107
Size: 4673
NgoMIV G'CCGG_C
Cuts at: 2852 2852
Size: 4673
NsiI A_TGCA'T
Cuts at: 583 583
Size: 4673
NspI r_CATG'y
Cuts at: 585 1296 1325 1726 2226 3133 585
Size: 711 29 401 500 907 2125
Fragments arranged by size:
2125 907 711 500 401 29
PacI TTA_AT'TAA
Cuts at: 287 287
Size: 4673
PciI A'CATG_T
Cuts at: 1292 1321 3129 1292
Size: 29 1808 2836
Fragments arranged by size:
2836 1808 29
PflMI CCAn_nnn'nTGG
Cuts at: 1044 1044
Size: 4673
PfoI T'CCnGG_A
Cuts at: 737 737
Size: 4673
PmeI GTTT'AAAC
Cuts at: 1672 1672
Size: 4673
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 155 187 3812 3844 155
Size: 32 3625 32 984
Fragments arranged by size:
3625 984 32 32
PshAI GACnn'nnGTC
Cuts at: 318 318
Size: 4673
PsiI TTA'TAA
Cuts at: 1172 1172
Size: 4673
PstI C_TGCA'G
Cuts at: 139 725 1720 2405 139
Size: 586 995 685 2407
Fragments arranged by size:
2407 995 685 586
PvuI CG_AT'CG
Cuts at: 104 104
Size: 4673
PvuII CAG'CTG
Cuts at: 442 2098 2458 442
Size: 1656 360 2657
Fragments arranged by size:
2657 1656 360
RsrII CG'GwC_CG
Cuts at: 2868 2868
Size: 4673
SacI G_AGCT'C
Cuts at: 1687 1687
Size: 4673
SalI G'TCGA_C
Cuts at: 1710 1710
Size: 4673
SapI GCTCTTCn'nnn_
Cuts at: 215 2696 2906 215
Size: 2481 210 1982
Fragments arranged by size:
2481 1982 210
SbfI CC_TGCA'GG
Cuts at: 1720 1720
Size: 4673
ScaI AGT'ACT
Cuts at: 4190 4190
Size: 4673
SexAI A'CCwGG_T
Cuts at: 115 115
Size: 4673
SfcI C'TryA_G
Cuts at: 33 135 721 1716 2401 3394 3585 33
Size: 102 586 995 685 993 191 1121
Fragments arranged by size:
1121 995 993 685 586 191 102
SfoI GGC'GCC
Cuts at: 2105 2353 2105
Size: 248 4425
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 4673
SmaI CCC'GGG
Cuts at: 1695 1695
Size: 4673
SmlI C'TyrA_G
Cuts at: 308 371 1828 2743 3235 3497 308
Size: 63 1457 915 492 262 1484
Fragments arranged by size:
1484 1457 915 492 262 63
SpeI A'CTAG_T
Cuts at: 67 67
Size: 4673
SphI G_CATG'C
Cuts at: 1726 2226 1726
Size: 500 4173
SspI AAT'ATT
Cuts at: 1251 1251
Size: 4673
StyI C'CwwG_G
Cuts at: 107 1502 1807 2161 107
Size: 1395 305 354 2619
Fragments arranged by size:
2619 1395 354 305
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 703 1673 2256 2389 2848 3057 703
Size: 970 583 133 459 209 2319
Fragments arranged by size:
2319 970 583 459 209 133
TatI w'GTAC_w
Cuts at: 695 4188 695
Size: 3493 1180
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 4190 4222 4190
Size: 32 4641
Tth111I GACn'n_nGTC
Cuts at: 540 2470 540
Size: 1930 2743
XbaI T'CTAG_A
Cuts at: 1704 1704
Size: 4673
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 1404 2168 1404
Size: 764 3909
XhoI C'TCGA_G
Cuts at: 371 371
Size: 4673
XmaI C'CCGG_G
Cuts at: 1693 1693
Size: 4673
Enzymes that do cut:
AccI Acc65I AclI AcuI AflIII AgeI AleI AloI
AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI BanI
BanII BbeI BbsI BciVI BclI BfrBI BglII BlpI
Bme1580I BmgBI BmrI BplI BpmI BpuEI BsaI BsaAI
BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsiEI BsiHKAI
Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BssSI
BstAPI BstBI BstYI BtgI BtgZI BtsI DraI DrdI
EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I
EcoRI FalI FspI HaeII Hin4I HincII KasI KpnI
MluI MmeI MscI MslI MspA1I NaeI NarI NcoI
NgoMIV NsiI NspI PacI PciI PflMI PfoI PmeI
PpiI PshAI PsiI PstI PvuI PvuII RsrII SacI
SalI SapI SbfI ScaI SexAI SfcI SfoI SgfI
SmaI SmlI SpeI SphI SspI StyI TaqII TatI
TstI Tth111I XbaI XcmI XhoI XmaI
Enzymes that do not cut:
AarI AatII AfeI AflII AhdI AlfI ApaI AscI
AvrII BaeI BbvCI BcgI BglI BmtI Bpu10I BsgI
BsiWI BsmI BsmBI BsrGI BssHII BstEII BstXI BstZ17I
Bsu36I ClaI CspCI DraIII EcoRV FseI FspAI HindIII
HpaI MfeI NdeI NheI NotI NruI PasI PmlI
PpuMI PspOMI PspXI PsrI SacII SanDI SfiI SgrAI
SnaBI SrfI StuI SwaI XmnI ZraI