Restriction map for
pF1KE0042
(Circular) (Six-base) MAPSORT of: pF1KE0042.seq Check: 2735 from: 1 to: 4646
pF1KE0042 4646 bp
With 182 enzymes: SgfI *
April 24, 2009 13:09 ..
AccI GT'mk_AC
Cuts at: 1684 1684
Size: 4646
Acc65I G'GTAC_C
Cuts at: 1662 1662
Size: 4646
AclI AA'CG_TT
Cuts at: 4486 4486
Size: 4646
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 397 2471 2903 3650 3918 397
Size: 2074 432 747 268 1125
Fragments arranged by size:
2074 1125 747 432 268
AflII C'TTAA_G
Cuts at: 1501 1501
Size: 4646
AflIII A'CryG_T
Cuts at: 1998 3102 4079 1998
Size: 1104 977 2565
Fragments arranged by size:
2565 1104 977
AgeI A'CCGG_T
Cuts at: 507 3034 507
Size: 2527 2119
AleI CACnn'nnGTG
Cuts at: 508 508
Size: 4646
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 415 447 415
Size: 32 4614
AlwNI CAG_nnn'CTG
Cuts at: 801 990 1548 1951 3518 801
Size: 189 558 403 1567 1929
Fragments arranged by size:
1929 1567 558 403 189
ApaLI G'TGCA_C
Cuts at: 3416 3416
Size: 4646
ApoI r'AATT_y
Cuts at: 1650 3999 4255 1650
Size: 2349 256 2041
Fragments arranged by size:
2349 2041 256
AseI AT'TA_AT
Cuts at: 20 20
Size: 4646
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 4646
AvaI C'yCGr_G
Cuts at: 779 1666 779
Size: 887 3759
BamHI G'GATC_C
Cuts at: 1671 1671
Size: 4646
BanI G'GyrC_C
Cuts at: 510 1662 2076 2324 2359 510
Size: 1152 414 248 35 2797
Fragments arranged by size:
2797 1152 414 248 35
BanII G_rGCy'C
Cuts at: 368 1481 1526 1556 1660 2690 368
Size: 1113 45 30 104 1030 2324
Fragments arranged by size:
2324 1113 1030 104 45 30
BbeI G_GCGC'C
Cuts at: 2080 2328 2080
Size: 248 4398
BbsI GAAGACnn'nnnn_
Cuts at: 286 1010 286
Size: 724 3922
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1369 1403 1369
Size: 34 4612
BciVI GTATCCnnnnn_n'
Cuts at: 2544 3305 4646 2544
Size: 761 1341 2544
Fragments arranged by size:
2544 1341 761
BclI T'GATC_A
Cuts at: 2166 2166
Size: 4646
BglII A'GATC_T
Cuts at: 653 2161 4198 653
Size: 1508 2037 1101
Fragments arranged by size:
2037 1508 1101
BlpI GC'TnA_GC
Cuts at: 1758 1758
Size: 4646
Bme1580I G_kGCm'C
Cuts at: 404 515 2271 2364 3420 404
Size: 111 1756 93 1056 1630
Fragments arranged by size:
1756 1630 1056 111 93
BmgBI CAC'GTC
Cuts at: 594 594
Size: 4646
BmrI ACTGGGnnnn_n'
Cuts at: 1858 1939 2274 4418 1858
Size: 81 335 2144 2086
Fragments arranged by size:
2144 2086 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 309 3013 309
Size: 2704 1942
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1156 1465 1570 1156
Size: 309 105 4232
Fragments arranged by size:
4232 309 105
Bpu10I CC'TnA_GC
Cuts at: 1183 1520 1183
Size: 337 4309
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1822 2701 3193 3491 1822
Size: 879 492 298 2977
Fragments arranged by size:
2977 879 492 298
BsaI GGTCTCn'nnnn_
Cuts at: 299 330 736 1326 4350 299
Size: 31 406 590 3024 595
Fragments arranged by size:
3024 595 590 406 31
BsaAI yAC'GTr
Cuts at: 768 1999 768
Size: 1231 3415
BsaBI GATnn'nnATC
Cuts at: 6 2160 4197 6
Size: 2154 2037 455
Fragments arranged by size:
2154 2037 455
BsaHI Gr'CG_yC
Cuts at: 1229 2077 2325 3027 4523 1229
Size: 848 248 702 1496 1352
Fragments arranged by size:
1496 1352 848 702 248
BsaWI w'CCGG_w
Cuts at: 339 507 1835 2356 3034 3308 3455 3866 339
Size: 168 1328 521 678 274 147 411 1119
Fragments arranged by size:
1328 1119 678 521 411 274 168 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1538 1568 1538
Size: 30 4616
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 336 799 965 1372 336
Size: 463 166 407 3610
Fragments arranged by size:
3610 463 407 166
BseYI C'CCAG_C
Cuts at: 2071 2829 3406 3902 2071
Size: 758 577 496 2815
Fragments arranged by size:
2815 758 577 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 864 892 1288 864
Size: 28 396 4222
Fragments arranged by size:
4222 396 28
BsiEI CG_ry'CG
Cuts at: 104 2188 2234 3442 104
Size: 2084 46 1208 1308
Fragments arranged by size:
2084 1308 1208 46
BsiHKAI G_wGCw'C
Cuts at: 1189 1481 1556 1660 2438 2628 3420 1189
Size: 292 75 104 778 190 792 2415
Fragments arranged by size:
2415 792 778 292 190 104 75
BsmI GAATG_Cn'
Cuts at: 472 472
Size: 4646
Bsp1286I G_dGCh'C
Cuts at: 368 404 515 1189 1481 1526 1556 1660 2271
Size: 36 111 674 292 45 30 104 611
Cuts at: 2271 2364 2438 2628 2690 3420 368
Size: 93 74 190 62 730 1594
Fragments arranged by size:
1594 730 674 611 292 190 111 104
93 74 62 45 36 30
BspEI T'CCGG_A
Cuts at: 3866 3866
Size: 4646
BspHI T'CATG_A
Cuts at: 1416 3822 1416
Size: 2406 2240
BspMI ACCTGCnnnn'nnnn_
Cuts at: 809 975 1567 1696 1910 2212 2593 809
Size: 166 592 129 214 302 381 2862
Fragments arranged by size:
2862 592 381 302 214 166 129
BsrBI CCG'CTC
Cuts at: 50 2938 2992 4476 4641 50
Size: 2888 54 1484 165 55
Fragments arranged by size:
2888 1484 165 55 54
BsrDI GCAATG_nn'
Cuts at: 2558 2558
Size: 4646
BsrFI r'CCGG_y
Cuts at: 507 2644 2825 3034 507
Size: 2137 181 209 2119
Fragments arranged by size:
2137 2119 209 181
BsrGI T'GTAC_A
Cuts at: 1083 1083
Size: 4646
BssSI C'ACGA_G
Cuts at: 2917 3275 2917
Size: 358 4288
BstAPI GCAn_nnn'nTGC
Cuts at: 801 2023 801
Size: 1222 3424
BstBI TT'CG_AA
Cuts at: 3007 3007
Size: 4646
BstXI CCAn_nnnn'nTGG
Cuts at: 316 316
Size: 4646
BstYI r'GATC_y
Cuts at: 1 653 1671 2138 2161 2496 2742 3754 3840
Size: 652 1018 467 23 335 246 1012 86
Cuts at: 3840 3852 4198 1
Size: 12 346 449
Fragments arranged by size:
1018 1012 652 467 449 346 335 246
86 23 12
Bsu36I CC'TnA_GG
Cuts at: 1009 1009
Size: 4646
BtgI C'CryG_G
Cuts at: 254 353 477 515 1260 254
Size: 99 124 38 745 3640
Fragments arranged by size:
3640 745 124 99 38
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 612 2596 2777 2940 612
Size: 1984 181 163 2318
Fragments arranged by size:
2318 1984 181 163
BtsI GCAGTG_nn'
Cuts at: 1629 1889 1629
Size: 260 4386
DraI TTT'AAA
Cuts at: 1645 1645
Size: 4646
DrdI GACnn_nn'nnGTC
Cuts at: 1306 2352 3210 1306
Size: 1046 858 2742
Fragments arranged by size:
2742 1046 858
EaeI y'GGCC_r
Cuts at: 318 350 944 2231 2405 2796 2823 4168 318
Size: 32 594 1287 174 391 27 1345 796
Fragments arranged by size:
1345 1287 796 594 391 174 32 27
EagI C'GGCC_G
Cuts at: 2231 2231
Size: 4646
EarI CTCTTCn'nnn_
Cuts at: 187 952 1349 2669 2879 187
Size: 765 397 1320 210 1954
Fragments arranged by size:
1954 1320 765 397 210
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 2854 3164 3310 4456 2854
Size: 310 146 1146 3044
Fragments arranged by size:
3044 1146 310 146
EcoICRI GAG'CTC
Cuts at: 1479 1554 1658 1479
Size: 75 104 4467
Fragments arranged by size:
4467 104 75
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 397 1156 1465 1570 2471 2903 3650 3918 397
Size: 759 309 105 901 432 747 268 1125
Fragments arranged by size:
1125 901 759 747 432 309 268 105
EcoNI CCTnn'n_nnAGG
Cuts at: 330 330
Size: 4646
EcoO109I rG'GnC_Cy
Cuts at: 1015 1308 1785 1015
Size: 293 477 3876
Fragments arranged by size:
3876 477 293
EcoRI G'AATT_C
Cuts at: 1650 1650
Size: 4646
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 249 1083 2665 249
Size: 834 1582 2230
Fragments arranged by size:
2230 1582 834
FspI TGC'GCA
Cuts at: 2427 2427
Size: 4646
HaeII r_GCGC'y
Cuts at: 541 906 2080 2328 3350 4329 541
Size: 365 1174 248 1022 979 858
Fragments arranged by size:
1174 1022 979 858 365 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1351 1383 2488 2518 2520 2550 1351
Size: 32 1105 30 2 30 3447
Fragments arranged by size:
3447 1105 32 30 30 2
HincII GTy'rAC
Cuts at: 1685 1685
Size: 4646
HindIII A'AGCT_T
Cuts at: 408 408
Size: 4646
KasI G'GCGC_C
Cuts at: 2076 2324 2076
Size: 248 4398
KpnI G_GTAC'C
Cuts at: 1666 1666
Size: 4646
MfeI C'AATT_G
Cuts at: 128 1339 128
Size: 1211 3435
MluI A'CGCG_T
Cuts at: 4079 4079
Size: 4646
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1586 3317 3501 1586
Size: 1731 184 2731
Fragments arranged by size:
2731 1731 184
MscI TGG'CCA
Cuts at: 320 946 2407 320
Size: 626 1461 2559
Fragments arranged by size:
2559 1461 626
MslI CAynn'nnrTG
Cuts at: 508 562 2635 2762 508
Size: 54 2073 127 2392
Fragments arranged by size:
2392 2073 127 54
MspA1I CmG'CkG
Cuts at: 676 1757 2071 2431 3444 3689 676
Size: 1081 314 360 1013 245 1633
Fragments arranged by size:
1633 1081 1013 360 314 245
NaeI GCC'GGC
Cuts at: 2827 2827
Size: 4646
NarI GG'CG_CC
Cuts at: 2077 2325 2077
Size: 248 4398
NcoI C'CATG_G
Cuts at: 353 477 1260 353
Size: 124 783 3739
Fragments arranged by size:
3739 783 124
NgoMIV G'CCGG_C
Cuts at: 2825 2825
Size: 4646
NspI r_CATG'y
Cuts at: 918 1500 1699 2199 3106 918
Size: 582 199 500 907 2458
Fragments arranged by size:
2458 907 582 500 199
PasI CC'CwG_GG
Cuts at: 1287 1314 1287
Size: 27 4619
PciI A'CATG_T
Cuts at: 3102 3102
Size: 4646
PflMI CCAn_nnn'nTGG
Cuts at: 420 478 1176 420
Size: 58 698 3890
Fragments arranged by size:
3890 698 58
PmeI GTTT'AAAC
Cuts at: 1645 1645
Size: 4646
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3785 3817 3785
Size: 32 4614
PpuMI rG'GwC_Cy
Cuts at: 1015 1308 1015
Size: 293 4353
PshAI GACnn'nnGTC
Cuts at: 286 286
Size: 4646
PstI C_TGCA'G
Cuts at: 1578 1693 2378 1578
Size: 115 685 3846
Fragments arranged by size:
3846 685 115
PvuI CG_AT'CG
Cuts at: 104 104
Size: 4646
PvuII CAG'CTG
Cuts at: 676 2071 2431 676
Size: 1395 360 2891
Fragments arranged by size:
2891 1395 360
RsrII CG'GwC_CG
Cuts at: 2841 2841
Size: 4646
SacI G_AGCT'C
Cuts at: 1481 1556 1660 1481
Size: 75 104 4467
Fragments arranged by size:
4467 104 75
SalI G'TCGA_C
Cuts at: 1683 1683
Size: 4646
SanDI GG'GwC_CC
Cuts at: 1015 1015
Size: 4646
SapI GCTCTTCn'nnn_
Cuts at: 2669 2879 2669
Size: 210 4436
SbfI CC_TGCA'GG
Cuts at: 1578 1693 1578
Size: 115 4531
ScaI AGT'ACT
Cuts at: 454 4163 454
Size: 3709 937
SfcI C'TryA_G
Cuts at: 33 1233 1574 1689 2374 3367 3558 33
Size: 1200 341 115 685 993 191 1121
Fragments arranged by size:
1200 1121 993 685 341 191 115
SfoI GGC'GCC
Cuts at: 2078 2326 2078
Size: 248 4398
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 4646
SgrAI Cr'CCGG_yG
Cuts at: 507 507
Size: 4646
SmaI CCC'GGG
Cuts at: 781 1668 781
Size: 887 3759
SmlI C'TyrA_G
Cuts at: 1501 1801 2716 3208 3470 1501
Size: 300 915 492 262 2677
Fragments arranged by size:
2677 915 492 300 262
SpeI A'CTAG_T
Cuts at: 67 577 67
Size: 510 4136
SphI G_CATG'C
Cuts at: 1699 2199 1699
Size: 500 4146
StuI AGG'CCT
Cuts at: 1141 1396 1141
Size: 255 4391
StyI C'CwwG_G
Cuts at: 353 477 620 1260 1325 1780 2134 353
Size: 124 143 640 65 455 354 2865
Fragments arranged by size:
2865 640 455 354 143 124 65
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 229 676 2229 2362 2821 3030 229
Size: 447 1553 133 459 209 1845
Fragments arranged by size:
1845 1553 459 447 209 133
TatI w'GTAC_w
Cuts at: 452 1083 4161 452
Size: 631 3078 937
Fragments arranged by size:
3078 937 631
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 167 199 901 933 4163 4195 167
Size: 32 702 32 3230 32 618
Fragments arranged by size:
3230 702 618 32 32 32
Tth111I GACn'n_nGTC
Cuts at: 2443 2443
Size: 4646
XbaI T'CTAG_A
Cuts at: 1677 1677
Size: 4646
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 114 252 2141 114
Size: 138 1889 2619
Fragments arranged by size:
2619 1889 138
XmaI C'CCGG_G
Cuts at: 779 1666 779
Size: 887 3759
XmnI GAAnn'nnTTC
Cuts at: 929 929
Size: 4646
Enzymes that do cut:
AccI Acc65I AclI AcuI AflII AflIII AgeI AleI
AloI AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI
BanI BanII BbeI BbsI BcgI BciVI BclI BglII
BlpI Bme1580I BmgBI BmrI BplI BpmI Bpu10I BpuEI
BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI
BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI
BsrBI BsrDI BsrFI BsrGI BssSI BstAPI BstBI BstXI
BstYI Bsu36I BtgI BtgZI BtsI DraI DrdI EaeI
EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI
FalI FspI HaeII Hin4I HincII HindIII KasI KpnI
MfeI MluI MmeI MscI MslI MspA1I NaeI NarI
NcoI NgoMIV NspI PasI PciI PflMI PmeI PpiI
PpuMI PshAI PstI PvuI PvuII RsrII SacI SalI
SanDI SapI SbfI ScaI SfcI SfoI SgfI SgrAI
SmaI SmlI SpeI SphI StuI StyI TaqII TatI
TstI Tth111I XbaI XcmI XmaI XmnI
Enzymes that do not cut:
AarI AatII AfeI AhdI AlfI ApaI AscI AvrII
BaeI BbvCI BfrBI BglI BmtI BsiWI BsmBI BssHII
BstEII BstZ17I ClaI CspCI DraIII EcoRV FseI FspAI
HpaI NdeI NheI NotI NruI NsiI PacI PfoI
PmlI PsiI PspOMI PspXI PsrI SacII SexAI SfiI
SnaBI SrfI SspI SwaI XhoI ZraI