Restriction map for
pF1KE0047
(Circular) (Six-base) MAPSORT of: pF1KE0047.seq Check: 9527 from: 1 to: 5372
pF1KE0047 5372 bp
With 182 enzymes: SgfI *
April 24, 2009 13:09 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 1263 1263
Size: 5372
AccI GT'mk_AC
Cuts at: 2410 2410
Size: 5372
Acc65I G'GTAC_C
Cuts at: 194 435 2388 194
Size: 241 1953 3178
Fragments arranged by size:
3178 1953 241
AclI AA'CG_TT
Cuts at: 5212 5212
Size: 5372
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 288 1403 1604 1802 2014 3197 3629 4376 4644
Size: 1115 201 198 212 1183 432 747 268
Cuts at: 4644 288
Size: 1016
Fragments arranged by size:
1183 1115 1016 747 432 268 212 201
198
AflIII A'CryG_T
Cuts at: 2364 2724 3828 4805 2364
Size: 360 1104 977 2931
Fragments arranged by size:
2931 1104 977 360
AgeI A'CCGG_T
Cuts at: 3760 3760
Size: 5372
AlwNI CAG_nnn'CTG
Cuts at: 1381 2345 2677 4244 1381
Size: 964 332 1567 2509
Fragments arranged by size:
2509 1567 964 332
ApaLI G'TGCA_C
Cuts at: 2360 4142 2360
Size: 1782 3590
ApoI r'AATT_y
Cuts at: 1594 1834 2376 4725 4981 1594
Size: 240 542 2349 256 1985
Fragments arranged by size:
2349 1985 542 256 240
AseI AT'TA_AT
Cuts at: 20 20
Size: 5372
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 5372
AvaI C'yCGr_G
Cuts at: 2392 2392
Size: 5372
AvrII C'CTAG_G
Cuts at: 1914 1914
Size: 5372
BamHI G'GATC_C
Cuts at: 2397 2397
Size: 5372
BanI G'GyrC_C
Cuts at: 118 194 435 1307 2388 2802 3050 3085 118
Size: 76 241 872 1081 414 248 35 2405
Fragments arranged by size:
2405 1081 872 414 248 241 76 35
BanII G_rGCy'C
Cuts at: 1123 1265 1285 1355 1791 2386 3416 1123
Size: 142 20 70 436 595 1030 3079
Fragments arranged by size:
3079 1030 595 436 142 70 20
BbeI G_GCGC'C
Cuts at: 2806 3054 2806
Size: 248 5124
BbvCI CC'TCA_GC
Cuts at: 1936 1936
Size: 5372
BciVI GTATCCnnnnn_n'
Cuts at: 3270 4031 5372 3270
Size: 761 1341 3270
Fragments arranged by size:
3270 1341 761
BclI T'GATC_A
Cuts at: 2106 2892 2106
Size: 786 4586
BglI GCCn_nnn'nGGC
Cuts at: 169 1185 2297 169
Size: 1016 1112 3244
Fragments arranged by size:
3244 1112 1016
BglII A'GATC_T
Cuts at: 1821 2887 4924 1821
Size: 1066 2037 2269
Fragments arranged by size:
2269 2037 1066
BlpI GC'TnA_GC
Cuts at: 1362 1959 2484 1362
Size: 597 525 4250
Fragments arranged by size:
4250 597 525
Bme1580I G_kGCm'C
Cuts at: 2364 2997 3090 4146 2364
Size: 633 93 1056 3590
Fragments arranged by size:
3590 1056 633 93
BmrI ACTGGGnnnn_n'
Cuts at: 1690 1893 2584 2665 3000 5144 1690
Size: 203 691 81 335 2144 1918
Fragments arranged by size:
2144 1918 691 335 203 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 1371 3739 1371
Size: 2368 3004
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 238 261 1030 1209 1315 1996 2281 238
Size: 23 769 179 106 681 285 3329
Fragments arranged by size:
3329 769 681 285 179 106 23
Bpu10I CC'TnA_GC
Cuts at: 1936 1936
Size: 5372
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1445 1896 2548 3427 3919 4217 1445
Size: 451 652 879 492 298 2600
Fragments arranged by size:
2600 879 652 492 451 298
BsaI GGTCTCn'nnnn_
Cuts at: 609 2074 5076 609
Size: 1465 3002 905
Fragments arranged by size:
3002 1465 905
BsaAI yAC'GTr
Cuts at: 207 2725 207
Size: 2518 2854
BsaBI GATnn'nnATC
Cuts at: 6 2886 4923 6
Size: 2880 2037 455
Fragments arranged by size:
2880 2037 455
BsaHI Gr'CG_yC
Cuts at: 2803 3051 3753 5249 2803
Size: 248 702 1496 2926
Fragments arranged by size:
2926 1496 702 248
BsaWI w'CCGG_w
Cuts at: 1336 2561 3082 3760 4034 4181 4592 1336
Size: 1225 521 678 274 147 411 2116
Fragments arranged by size:
2116 1225 678 521 411 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 512 542 1789 1819 1891 1921 1969 1999 512
Size: 30 1247 30 72 30 48 30 3885
Fragments arranged by size:
3885 1247 72 48 30 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 637 1047 1108 1363 1429 1505 1508 1786 637
Size: 410 61 255 66 76 3 278 4223
Fragments arranged by size:
4223 410 278 255 76 66 61 3
BseYI C'CCAG_C
Cuts at: 268 331 2797 3555 4132 4628 268
Size: 63 2466 758 577 496 1012
Fragments arranged by size:
2466 1012 758 577 496 63
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1109 1729 1109
Size: 620 4752
BsiEI CG_ry'CG
Cuts at: 104 2914 2960 4168 104
Size: 2810 46 1208 1308
Fragments arranged by size:
2810 1308 1208 46
BsiHKAI G_wGCw'C
Cuts at: 1123 1265 1285 1791 2364 2386 3164 3354 4146
Size: 142 20 506 573 22 778 190 792
Cuts at: 4146 1123
Size: 2349
Fragments arranged by size:
2349 792 778 573 506 190 142 22
20
BsmI GAATG_Cn'
Cuts at: 156 508 1579 2317 156
Size: 352 1071 738 3211
Fragments arranged by size:
3211 1071 738 352
Bsp1286I G_dGCh'C
Cuts at: 1123 1265 1285 1355 1791 2364 2386 2997 3090
Size: 142 20 70 436 573 22 611 93
Cuts at: 3090 3164 3354 3416 4146 1123
Size: 74 190 62 730 2349
Fragments arranged by size:
2349 730 611 573 436 190 142 93
74 70 62 22 20
BspEI T'CCGG_A
Cuts at: 4592 4592
Size: 5372
BspHI T'CATG_A
Cuts at: 4548 4548
Size: 5372
BspMI ACCTGCnnnn'nnnn_
Cuts at: 1208 1263 1416 2422 2636 2938 3319 1208
Size: 55 153 1006 214 302 381 3261
Fragments arranged by size:
3261 1006 381 302 214 153 55
BsrBI CCG'CTC
Cuts at: 50 1163 1305 1361 3664 3718 5202 5367 50
Size: 1113 142 56 2303 54 1484 165 55
Fragments arranged by size:
2303 1484 1113 165 142 56 55 54
BsrDI GCAATG_nn'
Cuts at: 3284 3284
Size: 5372
BsrFI r'CCGG_y
Cuts at: 197 1310 3370 3551 3760 197
Size: 1113 2060 181 209 1809
Fragments arranged by size:
2060 1809 1113 209 181
BssSI C'ACGA_G
Cuts at: 3643 4001 3643
Size: 358 5014
BstAPI GCAn_nnn'nTGC
Cuts at: 1235 2749 1235
Size: 1514 3858
BstBI TT'CG_AA
Cuts at: 3733 3733
Size: 5372
BstEII G'GTnAC_C
Cuts at: 1332 1728 1332
Size: 396 4976
BstYI r'GATC_y
Cuts at: 1 761 1821 2397 2864 2887 3222 3468 4480
Size: 760 1060 576 467 23 335 246 1012
Cuts at: 4480 4566 4578 4924 1
Size: 86 12 346 449
Fragments arranged by size:
1060 1012 760 576 467 449 346 335
246 86 23 12
BtgI C'CryG_G
Cuts at: 107 1141 1286 1454 1924 107
Size: 1034 145 168 470 3555
Fragments arranged by size:
3555 1034 470 168 145
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 2315 3322 3503 3666 2315
Size: 1007 181 163 4021
Fragments arranged by size:
4021 1007 181 163
BtsI GCAGTG_nn'
Cuts at: 420 2615 420
Size: 2195 3177
DraI TTT'AAA
Cuts at: 2371 2371
Size: 5372
DraIII CAC_nnn'GTG
Cuts at: 635 635
Size: 5372
DrdI GACnn_nn'nnGTC
Cuts at: 3078 3936 3078
Size: 858 4514
EaeI y'GGCC_r
Cuts at: 651 947 2003 2235 2957 3131 3522 3549 4894
Size: 296 1056 232 722 174 391 27 1345
Cuts at: 4894 651
Size: 1129
Fragments arranged by size:
1345 1129 1056 722 391 296 232 174
27
EagI C'GGCC_G
Cuts at: 2957 2957
Size: 5372
EarI CTCTTCn'nnn_
Cuts at: 1069 1093 1098 1111 1172 1179 3395 3605 1069
Size: 24 5 13 61 7 2216 210 2836
Fragments arranged by size:
2836 2216 210 61 24 13 7 5
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 1944 2260 3580 3890 4036 5182 1944
Size: 316 1320 310 146 1146 2134
Fragments arranged by size:
2134 1320 1146 316 310 146
EcoICRI GAG'CTC
Cuts at: 1121 1263 1283 1789 2384 1121
Size: 142 20 506 595 4109
Fragments arranged by size:
4109 595 506 142 20
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 238 261 288 1030 1209 1315 1403 1604 1802
Size: 23 27 742 179 106 88 201 198
Cuts at: 1802 1996 2014 2281 3197 3629 4376 4644 238
Size: 194 18 267 916 432 747 268 966
Fragments arranged by size:
966 916 747 742 432 268 267 201
198 194 179 106 88 27 23 18
EcoO109I rG'GnC_Cy
Cuts at: 1132 1220 1667 2511 1132
Size: 88 447 844 3993
Fragments arranged by size:
3993 844 447 88
EcoRI G'AATT_C
Cuts at: 1594 2376 1594
Size: 782 4590
EcoRV GAT'ATC
Cuts at: 1437 2247 1437
Size: 810 4562
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 3391 3391
Size: 5372
FspI TGC'GCA
Cuts at: 3153 3153
Size: 5372
HaeII r_GCGC'y
Cuts at: 204 2806 3054 4076 5055 204
Size: 2602 248 1022 979 521
Fragments arranged by size:
2602 1022 979 521 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 409 441 1969 2001 3214 3244 3246 3276 409
Size: 32 1528 32 1213 30 2 30 2505
Fragments arranged by size:
2505 1528 1213 32 32 30 30 2
HincII GTy'rAC
Cuts at: 2411 2411
Size: 5372
KasI G'GCGC_C
Cuts at: 2802 3050 2802
Size: 248 5124
KpnI G_GTAC'C
Cuts at: 198 439 2392 198
Size: 241 1953 3178
Fragments arranged by size:
3178 1953 241
MfeI C'AATT_G
Cuts at: 655 655
Size: 5372
MluI A'CGCG_T
Cuts at: 4805 4805
Size: 5372
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1661 2096 4043 4227 1661
Size: 435 1947 184 2806
Fragments arranged by size:
2806 1947 435 184
MscI TGG'CCA
Cuts at: 653 949 2005 2237 3133 653
Size: 296 1056 232 896 2892
Fragments arranged by size:
2892 1056 896 296 232
MslI CAynn'nnrTG
Cuts at: 1572 3361 3488 1572
Size: 1789 127 3456
Fragments arranged by size:
3456 1789 127
MspA1I CmG'CkG
Cuts at: 585 1241 1894 1947 2483 2797 3157 4170 4415
Size: 656 653 53 536 314 360 1013 245
Cuts at: 4415 585
Size: 1542
Fragments arranged by size:
1542 1013 656 653 536 360 314 245
53
NaeI GCC'GGC
Cuts at: 1312 3553 1312
Size: 2241 3131
NarI GG'CG_CC
Cuts at: 2803 3051 2803
Size: 248 5124
NcoI C'CATG_G
Cuts at: 107 1141 1454 1924 107
Size: 1034 313 470 3555
Fragments arranged by size:
3555 1034 470 313
NdeI CA'TA_TG
Cuts at: 714 839 714
Size: 125 5247
NgoMIV G'CCGG_C
Cuts at: 1310 3551 1310
Size: 2241 3131
NruI TCG'CGA
Cuts at: 2024 2024
Size: 5372
NspI r_CATG'y
Cuts at: 1551 1974 2425 2925 3832 1551
Size: 423 451 500 907 3091
Fragments arranged by size:
3091 907 500 451 423
PciI A'CATG_T
Cuts at: 3828 3828
Size: 5372
PflMI CCAn_nnn'nTGG
Cuts at: 1142 1142
Size: 5372
PfoI T'CCnGG_A
Cuts at: 2258 2258
Size: 5372
PmeI GTTT'AAAC
Cuts at: 2371 2371
Size: 5372
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4511 4543 4511
Size: 32 5340
PpuMI rG'GwC_Cy
Cuts at: 1132 1220 1132
Size: 88 5284
PsiI TTA'TAA
Cuts at: 683 683
Size: 5372
PstI C_TGCA'G
Cuts at: 1240 2350 2419 3104 1240
Size: 1110 69 685 3508
Fragments arranged by size:
3508 1110 685 69
PvuI CG_AT'CG
Cuts at: 104 104
Size: 5372
PvuII CAG'CTG
Cuts at: 585 1947 2797 3157 585
Size: 1362 850 360 2800
Fragments arranged by size:
2800 1362 850 360
RsrII CG'GwC_CG
Cuts at: 3567 3567
Size: 5372
SacI G_AGCT'C
Cuts at: 1123 1265 1285 1791 2386 1123
Size: 142 20 506 595 4109
Fragments arranged by size:
4109 595 506 142 20
SalI G'TCGA_C
Cuts at: 2409 2409
Size: 5372
SanDI GG'GwC_CC
Cuts at: 1132 1132
Size: 5372
SapI GCTCTTCn'nnn_
Cuts at: 1098 1111 3395 3605 1098
Size: 13 2284 210 2865
Fragments arranged by size:
2865 2284 210 13
SbfI CC_TGCA'GG
Cuts at: 2419 2419
Size: 5372
ScaI AGT'ACT
Cuts at: 4889 4889
Size: 5372
SfcI C'TryA_G
Cuts at: 33 257 1236 2346 2415 3100 4093 4284 33
Size: 224 979 1110 69 685 993 191 1121
Fragments arranged by size:
1121 1110 993 979 685 224 191 69
SfoI GGC'GCC
Cuts at: 2804 3052 2804
Size: 248 5124
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 5372
SmaI CCC'GGG
Cuts at: 2394 2394
Size: 5372
SmlI C'TyrA_G
Cuts at: 1460 1875 2527 3442 3934 4196 1460
Size: 415 652 915 492 262 2636
Fragments arranged by size:
2636 915 652 492 415 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 5372
SphI G_CATG'C
Cuts at: 2425 2925 2425
Size: 500 4872
StuI AGG'CCT
Cuts at: 1325 2266 1325
Size: 941 4431
StyI C'CwwG_G
Cuts at: 107 866 1141 1454 1914 1924 2006 2506 2860
Size: 759 275 313 460 10 82 500 354
Cuts at: 2860 107
Size: 2619
Fragments arranged by size:
2619 759 500 460 354 313 275 82
10
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 142 2955 3088 3547 3756 142
Size: 2813 133 459 209 1758
Fragments arranged by size:
2813 1758 459 209 133
TatI w'GTAC_w
Cuts at: 1979 4887 1979
Size: 2908 2464
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 4889 4921 4889
Size: 32 5340
Tth111I GACn'n_nGTC
Cuts at: 283 3169 283
Size: 2886 2486
XbaI T'CTAG_A
Cuts at: 2403 2403
Size: 5372
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 573 2867 573
Size: 2294 3078
XmaI C'CCGG_G
Cuts at: 2392 2392
Size: 5372
XmnI GAAnn'nnTTC
Cuts at: 2155 2155
Size: 5372
Enzymes that do cut:
AarI AccI Acc65I AclI AcuI AflIII AgeI AlwNI
ApaLI ApoI AseI AsiSI AvaI AvrII BamHI BanI
BanII BbeI BbvCI BciVI BclI BglI BglII BlpI
Bme1580I BmrI BplI BpmI Bpu10I BpuEI BsaI BsaAI
BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI
BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI
BsrFI BssSI BstAPI BstBI BstEII BstYI BtgI BtgZI
BtsI DraI DraIII DrdI EaeI EagI EarI EciI
EcoICRI Eco57MI EcoO109I EcoRI EcoRV FalI FspI HaeII
Hin4I HincII KasI KpnI MfeI MluI MmeI MscI
MslI MspA1I NaeI NarI NcoI NdeI NgoMIV NruI
NspI PciI PflMI PfoI PmeI PpiI PpuMI PsiI
PstI PvuI PvuII RsrII SacI SalI SanDI SapI
SbfI ScaI SfcI SfoI SgfI SmaI SmlI SpeI
SphI StuI StyI TaqII TatI TstI Tth111I XbaI
XcmI XmaI XmnI
Enzymes that do not cut:
AatII AfeI AflII AhdI AleI AlfI AloI ApaI
AscI BaeI BbsI BcgI BfrBI BmgBI BmtI BsiWI
BsmBI BsrGI BssHII BstXI BstZ17I Bsu36I ClaI CspCI
EcoNI FseI FspAI HindIII HpaI NheI NotI NsiI
PacI PasI PmlI PshAI PspOMI PspXI PsrI SacII
SexAI SfiI SgrAI SnaBI SrfI SspI SwaI XhoI
ZraI