Restriction map for
pF1KE0048
(Circular) (Six-base) MAPSORT of: pF1KE0048.seq Check: 7548 from: 1 to: 4679
pF1KE0048 4679 bp
With 182 enzymes: SgfI *
April 24, 2009 13:09 ..
AccI GT'mk_AC
Cuts at: 1189 1717 1189
Size: 528 4151
Acc65I G'GTAC_C
Cuts at: 342 1695 342
Size: 1353 3326
AclI AA'CG_TT
Cuts at: 4519 4519
Size: 4679
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 440 771 946 1298 1307 2504 2936 3683 3951
Size: 331 175 352 9 1197 432 747 268
Cuts at: 3951 440
Size: 1168
Fragments arranged by size:
1197 1168 747 432 352 331 268 175
9
AflIII A'CryG_T
Cuts at: 1517 2031 3135 4112 1517
Size: 514 1104 977 2084
Fragments arranged by size:
2084 1104 977 514
AgeI A'CCGG_T
Cuts at: 3067 3067
Size: 4679
AlwNI CAG_nnn'CTG
Cuts at: 1099 1279 1984 3551 1099
Size: 180 705 1567 2227
Fragments arranged by size:
2227 1567 705 180
ApaLI G'TGCA_C
Cuts at: 3449 3449
Size: 4679
ApoI r'AATT_y
Cuts at: 798 1107 1683 4032 4288 798
Size: 309 576 2349 256 1189
Fragments arranged by size:
2349 1189 576 309 256
AseI AT'TA_AT
Cuts at: 20 20
Size: 4679
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 4679
AvaI C'yCGr_G
Cuts at: 1523 1699 1523
Size: 176 4503
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'
Cuts at: 704 712 737 745 704
Size: 8 25 8 4638
Fragments arranged by size:
4638 25 8 8
BamHI G'GATC_C
Cuts at: 391 1704 391
Size: 1313 3366
BanI G'GyrC_C
Cuts at: 342 1510 1695 2109 2357 2392 342
Size: 1168 185 414 248 35 2629
Fragments arranged by size:
2629 1168 414 248 185 35
BanII G_rGCy'C
Cuts at: 1138 1693 2723 1138
Size: 555 1030 3094
Fragments arranged by size:
3094 1030 555
BbeI G_GCGC'C
Cuts at: 2113 2361 2113
Size: 248 4431
BbvCI CC'TCA_GC
Cuts at: 163 163
Size: 4679
BciVI GTATCCnnnnn_n'
Cuts at: 740 2577 3338 4679 740
Size: 1837 761 1341 740
Fragments arranged by size:
1837 1341 761 740
BclI T'GATC_A
Cuts at: 495 657 2199 495
Size: 162 1542 2975
Fragments arranged by size:
2975 1542 162
BglII A'GATC_T
Cuts at: 2194 4231 2194
Size: 2037 2642
BlpI GC'TnA_GC
Cuts at: 1791 1791
Size: 4679
Bme1580I G_kGCm'C
Cuts at: 2304 2397 3453 2304
Size: 93 1056 3530
Fragments arranged by size:
3530 1056 93
BmrI ACTGGGnnnn_n'
Cuts at: 1249 1891 1972 2307 4451 1249
Size: 642 81 335 2144 1477
Fragments arranged by size:
2144 1477 642 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3046 3046
Size: 4679
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1514 1514
Size: 4679
Bpu10I CC'TnA_GC
Cuts at: 163 163
Size: 4679
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 390 1855 2734 3226 3524 390
Size: 1465 879 492 298 1545
Fragments arranged by size:
1545 1465 879 492 298
BsaI GGTCTCn'nnnn_
Cuts at: 4383 4383
Size: 4679
BsaAI yAC'GTr
Cuts at: 2032 2032
Size: 4679
BsaBI GATnn'nnATC
Cuts at: 6 2193 4230 6
Size: 2187 2037 455
Fragments arranged by size:
2187 2037 455
BsaHI Gr'CG_yC
Cuts at: 2110 2358 3060 4556 2110
Size: 248 702 1496 2233
Fragments arranged by size:
2233 1496 702 248
BsaWI w'CCGG_w
Cuts at: 1868 2389 3067 3341 3488 3899 1868
Size: 521 678 274 147 411 2648
Fragments arranged by size:
2648 678 521 411 274 147
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 998 1511 998
Size: 513 4166
BseYI C'CCAG_C
Cuts at: 2104 2862 3439 3935 2104
Size: 758 577 496 2848
Fragments arranged by size:
2848 758 577 496
BsiEI CG_ry'CG
Cuts at: 104 2221 2267 3475 104
Size: 2117 46 1208 1308
Fragments arranged by size:
2117 1308 1208 46
BsiHKAI G_wGCw'C
Cuts at: 1693 2471 2661 3453 1693
Size: 778 190 792 2919
Fragments arranged by size:
2919 792 778 190
Bsp1286I G_dGCh'C
Cuts at: 1138 1693 2304 2397 2471 2661 2723 3453 1138
Size: 555 611 93 74 190 62 730 2364
Fragments arranged by size:
2364 730 611 555 190 93 74 62
BspEI T'CCGG_A
Cuts at: 3899 3899
Size: 4679
BspHI T'CATG_A
Cuts at: 3855 3855
Size: 4679
BspMI ACCTGCnnnn'nnnn_
Cuts at: 1729 1943 2245 2626 1729
Size: 214 302 381 3782
Fragments arranged by size:
3782 381 302 214
BsrBI CCG'CTC
Cuts at: 50 2971 3025 4509 4674 50
Size: 2921 54 1484 165 55
Fragments arranged by size:
2921 1484 165 55 54
BsrDI GCAATG_nn'
Cuts at: 745 1178 2591 745
Size: 433 1413 2833
Fragments arranged by size:
2833 1413 433
BsrFI r'CCGG_y
Cuts at: 2677 2858 3067 2677
Size: 181 209 4289
Fragments arranged by size:
4289 209 181
BsrGI T'GTAC_A
Cuts at: 720 720
Size: 4679
BssSI C'ACGA_G
Cuts at: 2950 3308 2950
Size: 358 4321
BstAPI GCAn_nnn'nTGC
Cuts at: 2056 2056
Size: 4679
BstBI TT'CG_AA
Cuts at: 3040 3040
Size: 4679
BstXI CCAn_nnnn'nTGG
Cuts at: 989 989
Size: 4679
BstYI r'GATC_y
Cuts at: 1 391 1587 1704 2171 2194 2529 2775 3787
Size: 390 1196 117 467 23 335 246 1012
Cuts at: 3787 3873 3885 4231 1
Size: 86 12 346 449
Fragments arranged by size:
1196 1012 467 449 390 346 335 246
117 86 23 12
BtgI C'CryG_G
Cuts at: 107 107
Size: 4679
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 2629 2810 2973 2629
Size: 181 163 4335
Fragments arranged by size:
4335 181 163
BtsI GCAGTG_nn'
Cuts at: 1023 1922 1023
Size: 899 3780
DraI TTT'AAA
Cuts at: 1678 1678
Size: 4679
DraIII CAC_nnn'GTG
Cuts at: 130 1018 130
Size: 888 3791
DrdI GACnn_nn'nnGTC
Cuts at: 2385 3243 2385
Size: 858 3821
EaeI y'GGCC_r
Cuts at: 118 991 1644 2264 2438 2829 2856 4201 118
Size: 873 653 620 174 391 27 1345 596
Fragments arranged by size:
1345 873 653 620 596 391 174 27
EagI C'GGCC_G
Cuts at: 2264 2264
Size: 4679
EarI CTCTTCn'nnn_
Cuts at: 200 558 576 1352 2702 2912 200
Size: 358 18 776 1350 210 1967
Fragments arranged by size:
1967 1350 776 358 210 18
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 2887 3197 3343 4489 2887
Size: 310 146 1146 3077
Fragments arranged by size:
3077 1146 310 146
EcoICRI GAG'CTC
Cuts at: 1691 1691
Size: 4679
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 440 771 946 1298 1307 1514 2504 2936 3683
Size: 331 175 352 9 207 990 432 747
Cuts at: 3683 3951 440
Size: 268 1168
Fragments arranged by size:
1168 990 747 432 352 331 268 207
175 9
EcoO109I rG'GnC_Cy
Cuts at: 1003 1818 1003
Size: 815 3864
EcoRI G'AATT_C
Cuts at: 1683 1683
Size: 4679
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 766 1354 2698 766
Size: 588 1344 2747
Fragments arranged by size:
2747 1344 588
FspI TGC'GCA
Cuts at: 2460 2460
Size: 4679
HaeII r_GCGC'y
Cuts at: 2113 2361 3383 4362 2113
Size: 248 1022 979 2430
Fragments arranged by size:
2430 1022 979 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 286 318 1038 1070 1195 1227 1446 1478 2521
Size: 32 720 32 125 32 219 32 1043
Cuts at: 2521 2551 2553 2583 286
Size: 30 2 30 2382
Fragments arranged by size:
2382 1043 720 219 125 32 32 32
32 30 30 2
HincII GTy'rAC
Cuts at: 415 1718 415
Size: 1303 3376
KasI G'GCGC_C
Cuts at: 2109 2357 2109
Size: 248 4431
KpnI G_GTAC'C
Cuts at: 346 1699 346
Size: 1353 3326
MluI A'CGCG_T
Cuts at: 4112 4112
Size: 4679
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 448 3350 3534 448
Size: 2902 184 1593
Fragments arranged by size:
2902 1593 184
MscI TGG'CCA
Cuts at: 120 993 1646 2440 120
Size: 873 653 794 2359
Fragments arranged by size:
2359 873 794 653
MslI CAynn'nnrTG
Cuts at: 356 1516 2668 2795 356
Size: 1160 1152 127 2240
Fragments arranged by size:
2240 1160 1152 127
MspA1I CmG'CkG
Cuts at: 1790 2104 2464 3477 3722 1790
Size: 314 360 1013 245 2747
Fragments arranged by size:
2747 1013 360 314 245
NaeI GCC'GGC
Cuts at: 2860 2860
Size: 4679
NarI GG'CG_CC
Cuts at: 2110 2358 2110
Size: 248 4431
NcoI C'CATG_G
Cuts at: 107 107
Size: 4679
NdeI CA'TA_TG
Cuts at: 627 627
Size: 4679
NgoMIV G'CCGG_C
Cuts at: 2858 2858
Size: 4679
NspI r_CATG'y
Cuts at: 1521 1732 2232 3139 1521
Size: 211 500 907 3061
Fragments arranged by size:
3061 907 500 211
PciI A'CATG_T
Cuts at: 1517 3135 1517
Size: 1618 3061
PflMI CCAn_nnn'nTGG
Cuts at: 1120 1120
Size: 4679
PfoI T'CCnGG_A
Cuts at: 394 1491 394
Size: 1097 3582
PmeI GTTT'AAAC
Cuts at: 1678 1678
Size: 4679
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3818 3850 3818
Size: 32 4647
PstI C_TGCA'G
Cuts at: 1726 2411 1726
Size: 685 3994
PvuI CG_AT'CG
Cuts at: 104 104
Size: 4679
PvuII CAG'CTG
Cuts at: 2104 2464 2104
Size: 360 4319
RsrII CG'GwC_CG
Cuts at: 2874 2874
Size: 4679
SacI G_AGCT'C
Cuts at: 1693 1693
Size: 4679
SalI G'TCGA_C
Cuts at: 1716 1716
Size: 4679
SapI GCTCTTCn'nnn_
Cuts at: 2702 2912 2702
Size: 210 4469
SbfI CC_TGCA'GG
Cuts at: 1726 1726
Size: 4679
ScaI AGT'ACT
Cuts at: 4196 4196
Size: 4679
SfcI C'TryA_G
Cuts at: 33 1722 2407 3400 3591 33
Size: 1689 685 993 191 1121
Fragments arranged by size:
1689 1121 993 685 191
SfoI GGC'GCC
Cuts at: 2111 2359 2111
Size: 248 4431
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 4679
SmaI CCC'GGG
Cuts at: 1701 1701
Size: 4679
SmlI C'TyrA_G
Cuts at: 405 1834 2749 3241 3503 405
Size: 1429 915 492 262 1581
Fragments arranged by size:
1581 1429 915 492 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 4679
SphI G_CATG'C
Cuts at: 1732 2232 1732
Size: 500 4179
SspI AAT'ATT
Cuts at: 781 781
Size: 4679
StyI C'CwwG_G
Cuts at: 107 168 1219 1813 2167 107
Size: 61 1051 594 354 2619
Fragments arranged by size:
2619 1051 594 354 61
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 1528 2262 2395 2854 3063 1528
Size: 734 133 459 209 3144
Fragments arranged by size:
3144 734 459 209 133
TatI w'GTAC_w
Cuts at: 720 4194 720
Size: 3474 1205
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 4196 4228 4196
Size: 32 4647
Tth111I GACn'n_nGTC
Cuts at: 2476 2476
Size: 4679
XbaI T'CTAG_A
Cuts at: 1177 1710 1177
Size: 533 4146
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2174 2174
Size: 4679
XmaI C'CCGG_G
Cuts at: 1699 1699
Size: 4679
Enzymes that do cut:
AccI Acc65I AclI AcuI AflIII AgeI AlwNI ApaLI
ApoI AseI AsiSI AvaI BaeI BamHI BanI BanII
BbeI BbvCI BciVI BclI BglII BlpI Bme1580I BmrI
BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI
BsaWI BseRI BseYI BsiEI BsiHKAI Bsp1286I BspEI BspHI
BspMI BsrBI BsrDI BsrFI BsrGI BssSI BstAPI BstBI
BstXI BstYI BtgI BtgZI BtsI DraI DraIII DrdI
EaeI EagI EarI EciI EcoICRI Eco57MI EcoO109I EcoRI
FalI FspI HaeII Hin4I HincII KasI KpnI MluI
MmeI MscI MslI MspA1I NaeI NarI NcoI NdeI
NgoMIV NspI PciI PflMI PfoI PmeI PpiI PstI
PvuI PvuII RsrII SacI SalI SapI SbfI ScaI
SfcI SfoI SgfI SmaI SmlI SpeI SphI SspI
StyI TaqII TatI TstI Tth111I XbaI XcmI XmaI
Enzymes that do not cut:
AarI AatII AfeI AflII AhdI AleI AlfI AloI
ApaI AscI AvrII BbsI BcgI BfrBI BglI BmgBI
BmtI BsaXI BsgI BsiWI BsmI BsmBI BssHII BstEII
BstZ17I Bsu36I ClaI CspCI EcoNI EcoRV FseI FspAI
HindIII HpaI MfeI NheI NotI NruI NsiI PacI
PasI PmlI PpuMI PshAI PsiI PspOMI PspXI PsrI
SacII SanDI SexAI SfiI SgrAI SnaBI SrfI StuI
SwaI XhoI XmnI ZraI