Restriction map for
pF1KE0059
(Circular) (Six-base) MAPSORT of: pF1KE0059.seq Check: 9336 from: 1 to: 4538
pF1KE0059 4538 bp
With 182 enzymes: SgfI *
April 24, 2009 13:09 ..
AccI GT'mk_AC
Cuts at: 1576 1576
Size: 4538
Acc65I G'GTAC_C
Cuts at: 1554 1554
Size: 4538
AclI AA'CG_TT
Cuts at: 4378 4378
Size: 4538
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 795 2363 2795 3542 3810 795
Size: 1568 432 747 268 1523
Fragments arranged by size:
1568 1523 747 432 268
AflIII A'CryG_T
Cuts at: 741 1890 2994 3971 741
Size: 1149 1104 977 1308
Fragments arranged by size:
1308 1149 1104 977
AgeI A'CCGG_T
Cuts at: 853 2926 853
Size: 2073 2465
AleI CACnn'nnGTG
Cuts at: 938 1190 1274 1442 938
Size: 252 84 168 4034
Fragments arranged by size:
4034 252 168 84
AlwNI CAG_nnn'CTG
Cuts at: 1843 3410 1843
Size: 1567 2971
ApaLI G'TGCA_C
Cuts at: 3308 3308
Size: 4538
ApoI r'AATT_y
Cuts at: 677 721 761 929 1013 1097 1181 1265 1349
Size: 44 40 168 84 84 84 84 84
Cuts at: 1349 1433 1517 1542 3891 4147 677
Size: 84 84 25 2349 256 1068
Fragments arranged by size:
2349 1068 256 168 84 84 84 84
84 84 84 44 40 25
AseI AT'TA_AT
Cuts at: 20 596 20
Size: 576 3962
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 4538
AvaI C'yCGr_G
Cuts at: 1558 1558
Size: 4538
BamHI G'GATC_C
Cuts at: 1563 1563
Size: 4538
BanI G'GyrC_C
Cuts at: 1554 1968 2216 2251 1554
Size: 414 248 35 3841
Fragments arranged by size:
3841 414 248 35
BanII G_rGCy'C
Cuts at: 299 991 1075 1243 1552 2582 299
Size: 692 84 168 309 1030 2255
Fragments arranged by size:
2255 1030 692 309 168 84
BbeI G_GCGC'C
Cuts at: 1972 2220 1972
Size: 248 4290
BbsI GAAGACnn'nnnn_
Cuts at: 611 611
Size: 4538
BbvCI CC'TCA_GC
Cuts at: 113 113
Size: 4538
BciVI GTATCCnnnnn_n'
Cuts at: 2436 3197 4538 2436
Size: 761 1341 2436
Fragments arranged by size:
2436 1341 761
BclI T'GATC_A
Cuts at: 2058 2058
Size: 4538
BglII A'GATC_T
Cuts at: 339 2053 4090 339
Size: 1714 2037 787
Fragments arranged by size:
2037 1714 787
BlpI GC'TnA_GC
Cuts at: 1158 1650 1158
Size: 492 4046
Bme1580I G_kGCm'C
Cuts at: 2163 2256 3312 2163
Size: 93 1056 3389
Fragments arranged by size:
3389 1056 93
BmrI ACTGGGnnnn_n'
Cuts at: 508 1750 1831 2166 4310 508
Size: 1242 81 335 2144 736
Fragments arranged by size:
2144 1242 736 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2905 2905
Size: 4538
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 435 435
Size: 4538
Bpu10I CC'TnA_GC
Cuts at: 113 257 113
Size: 144 4394
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 248 463 1714 2593 3085 3383 248
Size: 215 1251 879 492 298 1403
Fragments arranged by size:
1403 1251 879 492 298 215
BsaI GGTCTCn'nnnn_
Cuts at: 4242 4242
Size: 4538
BsaAI yAC'GTr
Cuts at: 1891 1891
Size: 4538
BsaBI GATnn'nnATC
Cuts at: 6 2052 4089 6
Size: 2046 2037 455
Fragments arranged by size:
2046 2037 455
BsaHI Gr'CG_yC
Cuts at: 1969 2217 2919 4415 1969
Size: 248 702 1496 2092
Fragments arranged by size:
2092 1496 702 248
BsaWI w'CCGG_w
Cuts at: 853 1727 2248 2926 3200 3347 3758 853
Size: 874 521 678 274 147 411 1633
Fragments arranged by size:
1633 874 678 521 411 274 147
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 175 175
Size: 4538
BseYI C'CCAG_C
Cuts at: 1963 2721 3298 3794 1963
Size: 758 577 496 2707
Fragments arranged by size:
2707 758 577 496
BsiEI CG_ry'CG
Cuts at: 104 2080 2126 3334 104
Size: 1976 46 1208 1308
Fragments arranged by size:
1976 1308 1208 46
BsiHKAI G_wGCw'C
Cuts at: 991 1075 1243 1552 2330 2520 3312 991
Size: 84 168 309 778 190 792 2217
Fragments arranged by size:
2217 792 778 309 190 168 84
Bsp1286I G_dGCh'C
Cuts at: 299 991 1075 1243 1552 2163 2256 2330 2520
Size: 692 84 168 309 611 93 74 190
Cuts at: 2520 2582 3312 299
Size: 62 730 1525
Fragments arranged by size:
1525 730 692 611 309 190 168 93
84 74 62
BspEI T'CCGG_A
Cuts at: 3758 3758
Size: 4538
BspHI T'CATG_A
Cuts at: 3714 3714
Size: 4538
BspMI ACCTGCnnnn'nnnn_
Cuts at: 1588 1802 2104 2485 1588
Size: 214 302 381 3641
Fragments arranged by size:
3641 381 302 214
BsrBI CCG'CTC
Cuts at: 50 2830 2884 4368 4533 50
Size: 2780 54 1484 165 55
Fragments arranged by size:
2780 1484 165 55 54
BsrDI GCAATG_nn'
Cuts at: 2450 2450
Size: 4538
BsrFI r'CCGG_y
Cuts at: 853 2536 2717 2926 853
Size: 1683 181 209 2465
Fragments arranged by size:
2465 1683 209 181
BssSI C'ACGA_G
Cuts at: 2809 3167 2809
Size: 358 4180
BstAPI GCAn_nnn'nTGC
Cuts at: 1915 1915
Size: 4538
BstBI TT'CG_AA
Cuts at: 2899 2899
Size: 4538
BstYI r'GATC_y
Cuts at: 1 339 1497 1563 2030 2053 2388 2634 3646
Size: 338 1158 66 467 23 335 246 1012
Cuts at: 3646 3732 3744 4090 1
Size: 86 12 346 449
Fragments arranged by size:
1158 1012 467 449 346 338 335 246
86 66 23 12
BtgI C'CryG_G
Cuts at: 107 107
Size: 4538
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 2488 2669 2832 2488
Size: 181 163 4194
Fragments arranged by size:
4194 181 163
BtsI GCAGTG_nn'
Cuts at: 1781 1781
Size: 4538
ClaI AT'CG_AT
Cuts at: 349 349
Size: 4538
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 1116 1151 1116
Size: 35 4503
DraI TTT'AAA
Cuts at: 1537 1537
Size: 4538
DrdI GACnn_nn'nnGTC
Cuts at: 2244 3102 2244
Size: 858 3680
EaeI y'GGCC_r
Cuts at: 2123 2297 2688 2715 4060 2123
Size: 174 391 27 1345 2601
Fragments arranged by size:
2601 1345 391 174 27
EagI C'GGCC_G
Cuts at: 2123 2123
Size: 4538
EarI CTCTTCn'nnn_
Cuts at: 2561 2771 2561
Size: 210 4328
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 2746 3056 3202 4348 2746
Size: 310 146 1146 2936
Fragments arranged by size:
2936 1146 310 146
EcoICRI GAG'CTC
Cuts at: 989 1073 1241 1550 989
Size: 84 168 309 3977
Fragments arranged by size:
3977 309 168 84
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 435 795 2363 2795 3542 3810 435
Size: 360 1568 432 747 268 1163
Fragments arranged by size:
1568 1163 747 432 360 268
EcoO109I rG'GnC_Cy
Cuts at: 1677 1677
Size: 4538
EcoRI G'AATT_C
Cuts at: 677 929 1013 1097 1181 1265 1349 1433 1517
Size: 252 84 84 84 84 84 84 84
Cuts at: 1517 1542 677
Size: 25 3673
Fragments arranged by size:
3673 252 84 84 84 84 84 84
84 25
EcoRV GAT'ATC
Cuts at: 505 505
Size: 4538
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2557 2557
Size: 4538
FspI TGC'GCA
Cuts at: 2319 2319
Size: 4538
HaeII r_GCGC'y
Cuts at: 1972 2220 3242 4221 1972
Size: 248 1022 979 2289
Fragments arranged by size:
2289 1022 979 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 331 363 2380 2410 2412 2442 331
Size: 32 2017 30 2 30 2427
Fragments arranged by size:
2427 2017 32 30 30 2
HincII GTy'rAC
Cuts at: 1255 1577 1255
Size: 322 4216
HindIII A'AGCT_T
Cuts at: 1078 1078
Size: 4538
KasI G'GCGC_C
Cuts at: 1968 2216 1968
Size: 248 4290
KpnI G_GTAC'C
Cuts at: 1558 1558
Size: 4538
MluI A'CGCG_T
Cuts at: 3971 3971
Size: 4538
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 194 3209 3393 194
Size: 3015 184 1339
Fragments arranged by size:
3015 1339 184
MscI TGG'CCA
Cuts at: 2299 2299
Size: 4538
MslI CAynn'nnrTG
Cuts at: 686 938 1022 1106 1190 1274 1358 1374 1442
Size: 252 84 84 84 84 84 16 68
Cuts at: 1442 2527 2654 686
Size: 1085 127 2570
Fragments arranged by size:
2570 1085 252 127 84 84 84 84
84 68 16
MspA1I CmG'CkG
Cuts at: 1649 1963 2323 3336 3581 1649
Size: 314 360 1013 245 2606
Fragments arranged by size:
2606 1013 360 314 245
NaeI GCC'GGC
Cuts at: 2719 2719
Size: 4538
NarI GG'CG_CC
Cuts at: 1969 2217 1969
Size: 248 4290
NcoI C'CATG_G
Cuts at: 107 107
Size: 4538
NdeI CA'TA_TG
Cuts at: 1371 1371
Size: 4538
NgoMIV G'CCGG_C
Cuts at: 2717 2717
Size: 4538
NspI r_CATG'y
Cuts at: 552 745 1009 1591 2091 2998 552
Size: 193 264 582 500 907 2092
Fragments arranged by size:
2092 907 582 500 264 193
PciI A'CATG_T
Cuts at: 741 2994 741
Size: 2253 2285
PmeI GTTT'AAAC
Cuts at: 1537 1537
Size: 4538
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3677 3709 3677
Size: 32 4506
PstI C_TGCA'G
Cuts at: 1585 2270 1585
Size: 685 3853
PvuI CG_AT'CG
Cuts at: 104 104
Size: 4538
PvuII CAG'CTG
Cuts at: 1963 2323 1963
Size: 360 4178
RsrII CG'GwC_CG
Cuts at: 2733 2733
Size: 4538
SacI G_AGCT'C
Cuts at: 991 1075 1243 1552 991
Size: 84 168 309 3977
Fragments arranged by size:
3977 309 168 84
SalI G'TCGA_C
Cuts at: 1575 1575
Size: 4538
SapI GCTCTTCn'nnn_
Cuts at: 2561 2771 2561
Size: 210 4328
SbfI CC_TGCA'GG
Cuts at: 1585 1585
Size: 4538
ScaI AGT'ACT
Cuts at: 448 4055 448
Size: 3607 931
SfcI C'TryA_G
Cuts at: 33 1581 2266 3259 3450 33
Size: 1548 685 993 191 1121
Fragments arranged by size:
1548 1121 993 685 191
SfoI GGC'GCC
Cuts at: 1970 2218 1970
Size: 248 4290
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 4538
SmaI CCC'GGG
Cuts at: 1560 1560
Size: 4538
SmlI C'TyrA_G
Cuts at: 263 442 1693 2608 3100 3362 263
Size: 179 1251 915 492 262 1439
Fragments arranged by size:
1439 1251 915 492 262 179
SpeI A'CTAG_T
Cuts at: 67 67
Size: 4538
SphI G_CATG'C
Cuts at: 1009 1591 2091 1009
Size: 582 500 3456
Fragments arranged by size:
3456 582 500
SspI AAT'ATT
Cuts at: 533 533
Size: 4538
StuI AGG'CCT
Cuts at: 329 640 808 892 1312 1396 329
Size: 311 168 84 420 84 3471
Fragments arranged by size:
3471 420 311 168 84 84
StyI C'CwwG_G
Cuts at: 107 1672 2026 107
Size: 1565 354 2619
Fragments arranged by size:
2619 1565 354
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 2121 2254 2713 2922 2121
Size: 133 459 209 3737
Fragments arranged by size:
3737 459 209 133
TatI w'GTAC_w
Cuts at: 446 4053 446
Size: 3607 931
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 337 369 1319 1351 4055 4087 337
Size: 32 950 32 2704 32 788
Fragments arranged by size:
2704 950 788 32 32 32
Tth111I GACn'n_nGTC
Cuts at: 2335 2335
Size: 4538
XbaI T'CTAG_A
Cuts at: 1569 1569
Size: 4538
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2033 2033
Size: 4538
XmaI C'CCGG_G
Cuts at: 1558 1558
Size: 4538
Enzymes that do cut:
AccI Acc65I AclI AcuI AflIII AgeI AleI AlwNI
ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII
BbeI BbsI BbvCI BciVI BclI BglII BlpI Bme1580I
BmrI BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI
BsaHI BsaWI BseRI BseYI BsiEI BsiHKAI Bsp1286I BspEI
BspHI BspMI BsrBI BsrDI BsrFI BssSI BstAPI BstBI
BstYI BtgI BtgZI BtsI ClaI CspCI DraI DrdI
EaeI EagI EarI EciI EcoICRI Eco57MI EcoO109I EcoRI
EcoRV FalI FspI HaeII Hin4I HincII HindIII KasI
KpnI MluI MmeI MscI MslI MspA1I NaeI NarI
NcoI NdeI NgoMIV NspI PciI PmeI PpiI PstI
PvuI PvuII RsrII SacI SalI SapI SbfI ScaI
SfcI SfoI SgfI SmaI SmlI SpeI SphI SspI
StuI StyI TaqII TatI TstI Tth111I XbaI XcmI
XmaI
Enzymes that do not cut:
AarI AatII AfeI AflII AhdI AlfI AloI ApaI
AscI AvrII BaeI BcgI BfrBI BglI BmgBI BmtI
BsaXI BsgI BsiWI BsmI BsmBI BsrGI BssHII BstEII
BstXI BstZ17I Bsu36I DraIII EcoNI FseI FspAI HpaI
MfeI NheI NotI NruI NsiI PacI PasI PflMI
PfoI PmlI PpuMI PshAI PsiI PspOMI PspXI PsrI
SacII SanDI SexAI SfiI SgrAI SnaBI SrfI SwaI
XhoI XmnI ZraI