Restriction map for
pF1KE0061
(Circular) (Six-base) MAPSORT of: pF1KE0061.seq Check: 2731 from: 1 to: 5924
pF1KE0061 5924 bp
With 182 enzymes: SgfI *
April 24, 2009 13:09 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 1752 1752
Size: 5924
AccI GT'mk_AC
Cuts at: 847 1486 2962 847
Size: 639 1476 3809
Fragments arranged by size:
3809 1476 639
Acc65I G'GTAC_C
Cuts at: 1204 2940 1204
Size: 1736 4188
AclI AA'CG_TT
Cuts at: 869 5764 869
Size: 4895 1029
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 2406 3749 4181 4928 5196 2406
Size: 1343 432 747 268 3134
Fragments arranged by size:
3134 1343 747 432 268
AfeI AGC'GCT
Cuts at: 1341 1341
Size: 5924
AflII C'TTAA_G
Cuts at: 999 999
Size: 5924
AflIII A'CryG_T
Cuts at: 1628 1919 2198 3276 4380 5357 1628
Size: 291 279 1078 1104 977 2195
Fragments arranged by size:
2195 1104 1078 977 291 279
AgeI A'CCGG_T
Cuts at: 1944 4312 1944
Size: 2368 3556
AleI CACnn'nnGTG
Cuts at: 2323 2323
Size: 5924
AlwNI CAG_nnn'CTG
Cuts at: 261 3229 4796 261
Size: 2968 1567 1389
Fragments arranged by size:
2968 1567 1389
ApaI G_GGCC'C
Cuts at: 2398 2398
Size: 5924
ApaLI G'TGCA_C
Cuts at: 1636 2527 4694 1636
Size: 891 2167 2866
Fragments arranged by size:
2866 2167 891
ApoI r'AATT_y
Cuts at: 313 366 2728 2928 5277 5533 313
Size: 53 2362 200 2349 256 704
Fragments arranged by size:
2362 2349 704 256 200 53
AseI AT'TA_AT
Cuts at: 20 20
Size: 5924
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 5924
AvaI C'yCGr_G
Cuts at: 214 766 1480 1811 2944 214
Size: 552 714 331 1133 3194
Fragments arranged by size:
3194 1133 714 552 331
BamHI G'GATC_C
Cuts at: 2949 2949
Size: 5924
BanI G'GyrC_C
Cuts at: 285 778 1204 1806 2130 2940 3354 3602 3637
Size: 493 426 602 324 810 414 248 35
Cuts at: 3637 285
Size: 2572
Fragments arranged by size:
2572 810 602 493 426 414 324 248
35
BanII G_rGCy'C
Cuts at: 628 1402 2016 2078 2398 2938 3968 628
Size: 774 614 62 320 540 1030 2584
Fragments arranged by size:
2584 1030 774 614 540 320 62
BbeI G_GCGC'C
Cuts at: 1810 3358 3606 1810
Size: 1548 248 4128
Fragments arranged by size:
4128 1548 248
BciVI GTATCCnnnnn_n'
Cuts at: 118 2604 3822 4583 5924 118
Size: 2486 1218 761 1341 118
Fragments arranged by size:
2486 1341 1218 761 118
BclI T'GATC_A
Cuts at: 2207 2574 3444 2207
Size: 367 870 4687
Fragments arranged by size:
4687 870 367
BglII A'GATC_T
Cuts at: 3439 5476 3439
Size: 2037 3887
BlpI GC'TnA_GC
Cuts at: 2277 3036 2277
Size: 759 5165
Bme1580I G_kGCm'C
Cuts at: 118 1640 2398 2531 3549 3642 4698 118
Size: 1522 758 133 1018 93 1056 1344
Fragments arranged by size:
1522 1344 1056 1018 758 133 93
BmgBI CAC'GTC
Cuts at: 468 651 1449 468
Size: 183 798 4943
Fragments arranged by size:
4943 798 183
BmrI ACTGGGnnnn_n'
Cuts at: 820 1057 3136 3217 3552 5696 820
Size: 237 2079 81 335 2144 1048
Fragments arranged by size:
2144 2079 1048 335 237 81
BmtI G_CTAG'C
Cuts at: 1427 1427
Size: 5924
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 214 4291 214
Size: 4077 1847
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 289 352 445 936 950 1711 2316 2621 289
Size: 63 93 491 14 761 605 305 3592
Fragments arranged by size:
3592 761 605 491 305 93 63 14
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 3100 3979 4471 4769 3100
Size: 879 492 298 4255
Fragments arranged by size:
4255 879 492 298
BsaI GGTCTCn'nnnn_
Cuts at: 2170 2824 5628 2170
Size: 654 2804 2466
Fragments arranged by size:
2804 2466 654
BsaAI yAC'GTr
Cuts at: 1629 1891 3277 1629
Size: 262 1386 4276
Fragments arranged by size:
4276 1386 262
BsaBI GATnn'nnATC
Cuts at: 6 3438 5475 6
Size: 3432 2037 455
Fragments arranged by size:
3432 2037 455
BsaHI Gr'CG_yC
Cuts at: 1193 1276 1807 2140 3355 3603 4305 5801 1193
Size: 83 531 333 1215 248 702 1496 1316
Fragments arranged by size:
1496 1316 1215 702 531 333 248 83
BsaWI w'CCGG_w
Cuts at: 498 723 1944 2704 3113 3634 4312 4586 4733
Size: 225 1221 760 409 521 678 274 147
Cuts at: 4733 5144 498
Size: 411 1278
Fragments arranged by size:
1278 1221 760 678 521 411 409 274
225 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 934 964 2482 2512 934
Size: 30 1518 30 4346
Fragments arranged by size:
4346 1518 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 232 400 1699 2551 2842 232
Size: 168 1299 852 291 3314
Fragments arranged by size:
3314 1299 852 291 168
BseYI C'CCAG_C
Cuts at: 507 2360 2428 3349 4107 4684 5180 507
Size: 1853 68 921 758 577 496 1251
Fragments arranged by size:
1853 1251 921 758 577 496 68
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 432 1913 432
Size: 1481 4443
BsiEI CG_ry'CG
Cuts at: 104 381 1955 3466 3512 4720 104
Size: 277 1574 1511 46 1208 1308
Fragments arranged by size:
1574 1511 1308 1208 277 46
BsiHKAI G_wGCw'C
Cuts at: 1053 1640 2278 2332 2386 2531 2938 3716 3906
Size: 587 638 54 54 145 407 778 190
Cuts at: 3906 4698 1053
Size: 792 2279
Fragments arranged by size:
2279 792 778 638 587 407 190 145
54 54
BsmI GAATG_Cn'
Cuts at: 2546 2546
Size: 5924
BsmBI CGTCTCn'nnnn_
Cuts at: 2017 2017
Size: 5924
Bsp1286I G_dGCh'C
Cuts at: 118 628 1053 1402 1640 2016 2078 2278 2332
Size: 510 425 349 238 376 62 200 54
Cuts at: 2332 2386 2398 2531 2938 3549 3642 3716 3906
Size: 54 12 133 407 611 93 74 190
Cuts at: 3906 3968 4698 118
Size: 62 730 1344
Fragments arranged by size:
1344 730 611 510 425 407 376 349
238 200 190 133 93 74 62 62
54 54 12
BspEI T'CCGG_A
Cuts at: 723 5144 723
Size: 4421 1503
BspHI T'CATG_A
Cuts at: 5100 5100
Size: 5924
BspMI ACCTGCnnnn'nnnn_
Cuts at: 441 660 1273 1752 2974 3188 3490 3871 441
Size: 219 613 479 1222 214 302 381 2494
Fragments arranged by size:
2494 1222 613 479 381 302 219 214
BsrBI CCG'CTC
Cuts at: 50 1649 4216 4270 5754 5919 50
Size: 1599 2567 54 1484 165 55
Fragments arranged by size:
2567 1599 1484 165 55 54
BsrDI GCAATG_nn'
Cuts at: 2162 3836 2162
Size: 1674 4250
BsrFI r'CCGG_y
Cuts at: 1794 1944 1954 3922 4103 4312 1794
Size: 150 10 1968 181 209 3406
Fragments arranged by size:
3406 1968 209 181 150 10
BsrGI T'GTAC_A
Cuts at: 1108 1985 2258 1108
Size: 877 273 4774
Fragments arranged by size:
4774 877 273
BssHII G'CGCG_C
Cuts at: 1611 1613 1715 1717 1611
Size: 2 102 2 5818
Fragments arranged by size:
5818 102 2 2
BssSI C'ACGA_G
Cuts at: 1681 2009 4195 4553 1681
Size: 328 2186 358 3052
Fragments arranged by size:
3052 2186 358 328
BstAPI GCAn_nnn'nTGC
Cuts at: 3301 3301
Size: 5924
BstBI TT'CG_AA
Cuts at: 4285 4285
Size: 5924
BstEII G'GTnAC_C
Cuts at: 1260 1380 1260
Size: 120 5804
BstXI CCAn_nnnn'nTGG
Cuts at: 2103 2238 2340 2103
Size: 135 102 5687
Fragments arranged by size:
5687 135 102
BstYI r'GATC_y
Cuts at: 1 324 1058 2743 2949 3416 3439 3774 4020
Size: 323 734 1685 206 467 23 335 246
Cuts at: 4020 5032 5118 5130 5476 1
Size: 1012 86 12 346 449
Fragments arranged by size:
1685 1012 734 467 449 346 335 323
246 206 86 23 12
BstZ17I GTA'TAC
Cuts at: 848 1487 848
Size: 639 5285
Bsu36I CC'TnA_GG
Cuts at: 2438 2438
Size: 5924
BtgI C'CryG_G
Cuts at: 107 318 1433 1852 2067 107
Size: 211 1115 419 215 3964
Fragments arranged by size:
3964 1115 419 215 211
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 234 516 1736 2196 3874 4055 4218 234
Size: 282 1220 460 1678 181 163 1940
Fragments arranged by size:
1940 1678 1220 460 282 181 163
BtsI GCAGTG_nn'
Cuts at: 1418 1798 1841 2587 3167 1418
Size: 380 43 746 580 4175
Fragments arranged by size:
4175 746 580 380 43
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 300 335 300
Size: 35 5889
DraI TTT'AAA
Cuts at: 2676 2923 2676
Size: 247 5677
DrdI GACnn_nn'nnGTC
Cuts at: 3630 4488 3630
Size: 858 5066
EaeI y'GGCC_r
Cuts at: 746 1442 1952 2124 2584 3509 3683 4074 4101
Size: 696 510 172 460 925 174 391 27
Cuts at: 4101 5446 746
Size: 1345 1224
Fragments arranged by size:
1345 1224 925 696 510 460 391 174
172 27
EagI C'GGCC_G
Cuts at: 1952 3509 1952
Size: 1557 4367
EarI CTCTTCn'nnn_
Cuts at: 3947 4157 3947
Size: 210 5714
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 292 414 1353 4132 4442 4588 5734 292
Size: 122 939 2779 310 146 1146 482
Fragments arranged by size:
2779 1146 939 482 310 146 122
EcoICRI GAG'CTC
Cuts at: 2936 2936
Size: 5924
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 289 352 445 936 950 1711 2316 2406 2621
Size: 63 93 491 14 761 605 90 215
Cuts at: 2621 3749 4181 4928 5196 289
Size: 1128 432 747 268 1017
Fragments arranged by size:
1128 1017 761 747 605 491 432 268
215 93 90 63 14
EcoNI CCTnn'n_nnAGG
Cuts at: 2437 2536 2437
Size: 99 5825
EcoO109I rG'GnC_Cy
Cuts at: 258 2394 2569 3063 258
Size: 2136 175 494 3119
Fragments arranged by size:
3119 2136 494 175
EcoRI G'AATT_C
Cuts at: 366 2928 366
Size: 2562 3362
EcoRV GAT'ATC
Cuts at: 591 591
Size: 5924
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 3943 3943
Size: 5924
FspI TGC'GCA
Cuts at: 3705 3705
Size: 5924
HaeII r_GCGC'y
Cuts at: 1343 1810 2284 3358 3606 4628 5607 1343
Size: 467 474 1074 248 1022 979 1660
Fragments arranged by size:
1660 1074 1022 979 474 467 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 562 594 961 993 1345 1377 1384 1416 1891
Size: 32 367 32 352 32 7 32 475
Cuts at: 1891 1923 3766 3796 3798 3828 562
Size: 32 1843 30 2 30 2658
Fragments arranged by size:
2658 1843 475 367 352 32 32 32
32 32 30 30 7 2
HincII GTy'rAC
Cuts at: 1157 1251 1899 2196 2655 2963 1157
Size: 94 648 297 459 308 4118
Fragments arranged by size:
4118 648 459 308 297 94
KasI G'GCGC_C
Cuts at: 1806 3354 3602 1806
Size: 1548 248 4128
Fragments arranged by size:
4128 1548 248
KpnI G_GTAC'C
Cuts at: 1208 2944 1208
Size: 1736 4188
MluI A'CGCG_T
Cuts at: 1919 5357 1919
Size: 3438 2486
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1655 4595 4779 1655
Size: 2940 184 2800
Fragments arranged by size:
2940 2800 184
MscI TGG'CCA
Cuts at: 2586 3685 2586
Size: 1099 4825
MslI CAynn'nnrTG
Cuts at: 899 2323 3913 4040 899
Size: 1424 1590 127 2783
Fragments arranged by size:
2783 1590 1424 127
MspA1I CmG'CkG
Cuts at: 447 1854 2666 3035 3349 3709 4722 4967 447
Size: 1407 812 369 314 360 1013 245 1404
Fragments arranged by size:
1407 1404 1013 812 369 360 314 245
NaeI GCC'GGC
Cuts at: 1796 4105 1796
Size: 2309 3615
NarI GG'CG_CC
Cuts at: 1807 3355 3603 1807
Size: 1548 248 4128
Fragments arranged by size:
4128 1548 248
NcoI C'CATG_G
Cuts at: 107 107
Size: 5924
NdeI CA'TA_TG
Cuts at: 1028 1028
Size: 5924
NgoMIV G'CCGG_C
Cuts at: 1794 4103 1794
Size: 2309 3615
NheI G'CTAG_C
Cuts at: 1423 1423
Size: 5924
NspI r_CATG'y
Cuts at: 2303 2517 2977 3477 4384 2303
Size: 214 460 500 907 3843
Fragments arranged by size:
3843 907 500 460 214
PasI CC'CwG_GG
Cuts at: 133 133
Size: 5924
PciI A'CATG_T
Cuts at: 4380 4380
Size: 5924
PflMI CCAn_nnn'nTGG
Cuts at: 1878 1878
Size: 5924
PfoI T'CCnGG_A
Cuts at: 644 2562 644
Size: 1918 4006
PmeI GTTT'AAAC
Cuts at: 2923 2923
Size: 5924
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 5063 5095 5063
Size: 32 5892
PpuMI rG'GwC_Cy
Cuts at: 258 258
Size: 5924
PsiI TTA'TAA
Cuts at: 163 163
Size: 5924
PspOMI G'GGCC_C
Cuts at: 2394 2394
Size: 5924
PsrI GAACnnnnnnTACnnnnnnn_nnnnn'
Cuts at: 831 863 1477 1509 831
Size: 32 614 32 5246
Fragments arranged by size:
5246 614 32 32
PstI C_TGCA'G
Cuts at: 347 452 1670 1763 2971 3656 347
Size: 105 1218 93 1208 685 2615
Fragments arranged by size:
2615 1218 1208 685 105 93
PvuI CG_AT'CG
Cuts at: 104 104
Size: 5924
PvuII CAG'CTG
Cuts at: 2666 3349 3709 2666
Size: 683 360 4881
Fragments arranged by size:
4881 683 360
RsrII CG'GwC_CG
Cuts at: 4119 4119
Size: 5924
SacI G_AGCT'C
Cuts at: 2938 2938
Size: 5924
SacII CC_GC'GG
Cuts at: 1855 1855
Size: 5924
SalI G'TCGA_C
Cuts at: 2961 2961
Size: 5924
SapI GCTCTTCn'nnn_
Cuts at: 3947 4157 3947
Size: 210 5714
SbfI CC_TGCA'GG
Cuts at: 2971 2971
Size: 5924
ScaI AGT'ACT
Cuts at: 5441 5441
Size: 5924
SexAI A'CCwGG_T
Cuts at: 1200 2915 1200
Size: 1715 4209
SfcI C'TryA_G
Cuts at: 33 343 448 1666 1759 2453 2967 3652 4645
Size: 310 105 1218 93 694 514 685 993
Cuts at: 4645 4836 33
Size: 191 1121
Fragments arranged by size:
1218 1121 993 694 685 514 310 191
105 93
SfoI GGC'GCC
Cuts at: 1808 3356 3604 1808
Size: 1548 248 4128
Fragments arranged by size:
4128 1548 248
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 5924
SmaI CCC'GGG
Cuts at: 2946 2946
Size: 5924
SmlI C'TyrA_G
Cuts at: 999 3079 3994 4486 4748 999
Size: 2080 915 492 262 2175
Fragments arranged by size:
2175 2080 915 492 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 5924
SphI G_CATG'C
Cuts at: 2517 2977 3477 2517
Size: 460 500 4964
Fragments arranged by size:
4964 500 460
StyI C'CwwG_G
Cuts at: 107 3058 3412 107
Size: 2951 354 2619
Fragments arranged by size:
2951 2619 354
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 198 1250 1807 2194 2415 3507 3640 4099 4308
Size: 1052 557 387 221 1092 133 459 209
Cuts at: 4308 198
Size: 1814
Fragments arranged by size:
1814 1092 1052 557 459 387 221 209
133
TatI w'GTAC_w
Cuts at: 1108 1985 2258 5439 1108
Size: 877 273 3181 1593
Fragments arranged by size:
3181 1593 877 273
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 872 904 1043 1075 5441 5473 872
Size: 32 139 32 4366 32 1323
Fragments arranged by size:
4366 1323 139 32 32 32
Tth111I GACn'n_nGTC
Cuts at: 3721 3721
Size: 5924
XbaI T'CTAG_A
Cuts at: 2955 2955
Size: 5924
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 987 3419 987
Size: 2432 3492
XmaI C'CCGG_G
Cuts at: 2944 2944
Size: 5924
XmnI GAAnn'nnTTC
Cuts at: 526 526
Size: 5924
Enzymes that do cut:
AarI AccI Acc65I AclI AcuI AfeI AflII AflIII
AgeI AleI AlwNI ApaI ApaLI ApoI AseI AsiSI
AvaI BamHI BanI BanII BbeI BciVI BclI BglII
BlpI Bme1580I BmgBI BmrI BmtI BplI BpmI BpuEI
BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI
BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI
BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI
BstBI BstEII BstXI BstYI BstZ17I Bsu36I BtgI BtgZI
BtsI CspCI DraI DrdI EaeI EagI EarI EciI
EcoICRI Eco57MI EcoNI EcoO109I EcoRI EcoRV FalI FspI
HaeII Hin4I HincII KasI KpnI MluI MmeI MscI
MslI MspA1I NaeI NarI NcoI NdeI NgoMIV NheI
NspI PasI PciI PflMI PfoI PmeI PpiI PpuMI
PsiI PspOMI PsrI PstI PvuI PvuII RsrII SacI
SacII SalI SapI SbfI ScaI SexAI SfcI SfoI
SgfI SmaI SmlI SpeI SphI StyI TaqII TatI
TstI Tth111I XbaI XcmI XmaI XmnI
Enzymes that do not cut:
AatII AhdI AlfI AloI AscI AvrII BaeI BbsI
BbvCI BcgI BfrBI BglI Bpu10I BsiWI ClaI DraIII
FseI FspAI HindIII HpaI MfeI NotI NruI NsiI
PacI PmlI PshAI PspXI SanDI SfiI SgrAI SnaBI
SrfI SspI StuI SwaI XhoI ZraI