Restriction map for
pF1KE0064
(Circular) (Six-base) MAPSORT of: pF1KE0064.seq Check: 9942 from: 1 to: 4850
pF1KE0064 4850 bp
With 182 enzymes: SgfI *
April 24, 2009 13:09 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 336 336
Size: 4850
AccI GT'mk_AC
Cuts at: 1888 1888
Size: 4850
Acc65I G'GTAC_C
Cuts at: 1866 1866
Size: 4850
AclI AA'CG_TT
Cuts at: 4690 4690
Size: 4850
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 992 2675 3107 3854 4122 992
Size: 1683 432 747 268 1720
Fragments arranged by size:
1720 1683 747 432 268
AflIII A'CryG_T
Cuts at: 1224 2202 3306 4283 1224
Size: 978 1104 977 1791
Fragments arranged by size:
1791 1104 978 977
AgeI A'CCGG_T
Cuts at: 3238 3238
Size: 4850
AleI CACnn'nnGTG
Cuts at: 319 385 1131 319
Size: 66 746 4038
Fragments arranged by size:
4038 746 66
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 970 970
Size: 4850
AlwNI CAG_nnn'CTG
Cuts at: 755 1031 2155 3722 755
Size: 276 1124 1567 1883
Fragments arranged by size:
1883 1567 1124 276
ApaI G_GGCC'C
Cuts at: 1618 1618
Size: 4850
ApaLI G'TGCA_C
Cuts at: 3620 3620
Size: 4850
ApoI r'AATT_y
Cuts at: 1854 4203 4459 1854
Size: 2349 256 2245
Fragments arranged by size:
2349 2245 256
AseI AT'TA_AT
Cuts at: 20 20
Size: 4850
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 4850
AvaI C'yCGr_G
Cuts at: 1870 1870
Size: 4850
AvrII C'CTAG_G
Cuts at: 1644 1644
Size: 4850
BamHI G'GATC_C
Cuts at: 1875 1875
Size: 4850
BanI G'GyrC_C
Cuts at: 1198 1554 1866 2280 2528 2563 1198
Size: 356 312 414 248 35 3485
Fragments arranged by size:
3485 414 356 312 248 35
BanII G_rGCy'C
Cuts at: 823 938 1577 1618 1864 2894 823
Size: 115 639 41 246 1030 2779
Fragments arranged by size:
2779 1030 639 246 115 41
BbeI G_GCGC'C
Cuts at: 1202 1558 2284 2532 1202
Size: 356 726 248 3520
Fragments arranged by size:
3520 726 356 248
BbvCI CC'TCA_GC
Cuts at: 584 1216 584
Size: 632 4218
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 835 869 835
Size: 34 4816
BciVI GTATCCnnnnn_n'
Cuts at: 1415 2748 3509 4850 1415
Size: 1333 761 1341 1415
Fragments arranged by size:
1415 1341 1333 761
BclI T'GATC_A
Cuts at: 2370 2370
Size: 4850
BfrBI ATG'CAT
Cuts at: 1346 1346
Size: 4850
BglII A'GATC_T
Cuts at: 2365 4402 2365
Size: 2037 2813
BlpI GC'TnA_GC
Cuts at: 1962 1962
Size: 4850
Bme1580I G_kGCm'C
Cuts at: 803 1618 2475 2568 3624 803
Size: 815 857 93 1056 2029
Fragments arranged by size:
2029 1056 857 815 93
BmgBI CAC'GTC
Cuts at: 1069 1237 1321 1327 1489 1069
Size: 168 84 6 162 4430
Fragments arranged by size:
4430 168 162 84 6
BmrI ACTGGGnnnn_n'
Cuts at: 259 547 642 681 720 798 2062 2143 2478
Size: 288 95 39 39 78 1264 81 335
Cuts at: 2478 4622 259
Size: 2144 487
Fragments arranged by size:
2144 1264 487 335 288 95 81 78
39 39
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 957 1113 3217 957
Size: 156 2104 2590
Fragments arranged by size:
2590 2104 156
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 500 927 1832 500
Size: 427 905 3518
Fragments arranged by size:
3518 905 427
Bpu10I CC'TnA_GC
Cuts at: 584 1211 1216 584
Size: 627 5 4218
Fragments arranged by size:
4218 627 5
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2026 2905 3397 3695 2026
Size: 879 492 298 3181
Fragments arranged by size:
3181 879 492 298
BsaI GGTCTCn'nnnn_
Cuts at: 1634 1784 4554 1634
Size: 150 2770 1930
Fragments arranged by size:
2770 1930 150
BsaAI yAC'GTr
Cuts at: 465 1227 2203 465
Size: 762 976 3112
Fragments arranged by size:
3112 976 762
BsaBI GATnn'nnATC
Cuts at: 6 2364 4401 6
Size: 2358 2037 455
Fragments arranged by size:
2358 2037 455
BsaHI Gr'CG_yC
Cuts at: 609 1161 1199 1266 1555 2281 2529 3231 4727
Size: 552 38 67 289 726 248 702 1496
Cuts at: 4727 609
Size: 732
Fragments arranged by size:
1496 732 726 702 552 289 248 67
38
BsaWI w'CCGG_w
Cuts at: 2039 2560 3238 3512 3659 4070 2039
Size: 521 678 274 147 411 2819
Fragments arranged by size:
2819 678 521 411 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1226 1256 1671 1701 1226
Size: 30 415 30 4375
Fragments arranged by size:
4375 415 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 171 572 869 872 171
Size: 401 297 3 4149
Fragments arranged by size:
4149 401 297 3
BseYI C'CCAG_C
Cuts at: 1600 1610 2275 3033 3610 4106 1600
Size: 10 665 758 577 496 2344
Fragments arranged by size:
2344 758 665 577 496 10
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 487 975 487
Size: 488 4362
BsiEI CG_ry'CG
Cuts at: 104 2392 2438 3646 104
Size: 2288 46 1208 1308
Fragments arranged by size:
2288 1308 1208 46
BsiHKAI G_wGCw'C
Cuts at: 516 862 1514 1864 2642 2832 3624 516
Size: 346 652 350 778 190 792 1742
Fragments arranged by size:
1742 792 778 652 350 346 190
BsmI GAATG_Cn'
Cuts at: 553 553
Size: 4850
BsmBI CGTCTCn'nnnn_
Cuts at: 1059 1059
Size: 4850
Bsp1286I G_dGCh'C
Cuts at: 516 803 823 862 938 1514 1577 1618 1864
Size: 287 20 39 76 576 63 41 246
Cuts at: 1864 2475 2568 2642 2832 2894 3624 516
Size: 611 93 74 190 62 730 1742
Fragments arranged by size:
1742 730 611 576 287 246 190 93
76 74 63 62 41 39 20
BspEI T'CCGG_A
Cuts at: 4070 4070
Size: 4850
BspHI T'CATG_A
Cuts at: 1653 4026 1653
Size: 2373 2477
BspMI ACCTGCnnnn'nnnn_
Cuts at: 336 715 962 1107 1900 2114 2416 2797 336
Size: 379 247 145 793 214 302 381 2389
Fragments arranged by size:
2389 793 381 379 302 247 214 145
BsrBI CCG'CTC
Cuts at: 50 144 851 1007 1166 1767 3142 3196 4680
Size: 94 707 156 159 601 1375 54 1484
Cuts at: 4680 4845 50
Size: 165 55
Fragments arranged by size:
1484 1375 707 601 165 159 156 94
55 54
BsrDI GCAATG_nn'
Cuts at: 1806 2762 1806
Size: 956 3894
BsrFI r'CCGG_y
Cuts at: 1551 2848 3029 3238 1551
Size: 1297 181 209 3163
Fragments arranged by size:
3163 1297 209 181
BssSI C'ACGA_G
Cuts at: 3121 3479 3121
Size: 358 4492
BstAPI GCAn_nnn'nTGC
Cuts at: 1834 2227 1834
Size: 393 4457
BstBI TT'CG_AA
Cuts at: 3211 3211
Size: 4850
BstXI CCAn_nnnn'nTGG
Cuts at: 1177 1177
Size: 4850
BstYI r'GATC_y
Cuts at: 1 125 1875 2342 2365 2700 2946 3958 4044
Size: 124 1750 467 23 335 246 1012 86
Cuts at: 4044 4056 4402 1
Size: 12 346 449
Fragments arranged by size:
1750 1012 467 449 346 335 246 124
86 23 12
Bsu36I CC'TnA_GG
Cuts at: 629 629
Size: 4850
BtgI C'CryG_G
Cuts at: 1261 1438 1785 1261
Size: 177 347 4326
Fragments arranged by size:
4326 347 177
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 1314 2800 2981 3144 1314
Size: 1486 181 163 3020
Fragments arranged by size:
3020 1486 181 163
BtsI GCAGTG_nn'
Cuts at: 475 623 1291 1474 2093 475
Size: 148 668 183 619 3232
Fragments arranged by size:
3232 668 619 183 148
DraI TTT'AAA
Cuts at: 1849 1849
Size: 4850
DraIII CAC_nnn'GTG
Cuts at: 650 650
Size: 4850
DrdI GACnn_nn'nnGTC
Cuts at: 2556 3414 2556
Size: 858 3992
EaeI y'GGCC_r
Cuts at: 1441 1782 2435 2609 3000 3027 4372 1441
Size: 341 653 174 391 27 1345 1919
Fragments arranged by size:
1919 1345 653 391 341 174 27
EagI C'GGCC_G
Cuts at: 2435 2435
Size: 4850
EarI CTCTTCn'nnn_
Cuts at: 2873 3083 2873
Size: 210 4640
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 3058 3368 3514 4660 3058
Size: 310 146 1146 3248
Fragments arranged by size:
3248 1146 310 146
EcoICRI GAG'CTC
Cuts at: 1862 1862
Size: 4850
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 500 927 992 1832 2675 3107 3854 4122 500
Size: 427 65 840 843 432 747 268 1228
Fragments arranged by size:
1228 843 840 747 432 427 268 65
EcoNI CCTnn'n_nnAGG
Cuts at: 1460 1460
Size: 4850
EcoO109I rG'GnC_Cy
Cuts at: 166 1614 1989 166
Size: 1448 375 3027
Fragments arranged by size:
3027 1448 375
EcoRI G'AATT_C
Cuts at: 1854 1854
Size: 4850
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2869 2869
Size: 4850
FspI TGC'GCA
Cuts at: 2631 2631
Size: 4850
HaeII r_GCGC'y
Cuts at: 1202 1558 2284 2532 3554 4533 1202
Size: 356 726 248 1022 979 1519
Fragments arranged by size:
1519 1022 979 726 356 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 2692 2722 2724 2754 2692
Size: 30 2 30 4788
Fragments arranged by size:
4788 30 30 2
HincII GTy'rAC
Cuts at: 1889 1889
Size: 4850
HindIII A'AGCT_T
Cuts at: 1248 1248
Size: 4850
KasI G'GCGC_C
Cuts at: 1198 1554 2280 2528 1198
Size: 356 726 248 3520
Fragments arranged by size:
3520 726 356 248
KpnI G_GTAC'C
Cuts at: 1870 1870
Size: 4850
MluI A'CGCG_T
Cuts at: 4283 4283
Size: 4850
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 171 1456 3521 3705 171
Size: 1285 2065 184 1316
Fragments arranged by size:
2065 1316 1285 184
MscI TGG'CCA
Cuts at: 1784 2611 1784
Size: 827 4023
MslI CAynn'nnrTG
Cuts at: 319 385 1131 1491 2839 2966 319
Size: 66 746 360 1348 127 2203
Fragments arranged by size:
2203 1348 746 360 127 66
MspA1I CmG'CkG
Cuts at: 755 911 1440 1961 2275 2635 3648 3893 755
Size: 156 529 521 314 360 1013 245 1712
Fragments arranged by size:
1712 1013 529 521 360 314 245 156
NaeI GCC'GGC
Cuts at: 1553 3031 1553
Size: 1478 3372
NarI GG'CG_CC
Cuts at: 1199 1555 2281 2529 1199
Size: 356 726 248 3520
Fragments arranged by size:
3520 726 356 248
NcoI C'CATG_G
Cuts at: 1785 1785
Size: 4850
NgoMIV G'CCGG_C
Cuts at: 1551 3029 1551
Size: 1478 3372
NsiI A_TGCA'T
Cuts at: 1348 1348
Size: 4850
NspI r_CATG'y
Cuts at: 1903 2403 3310 1903
Size: 500 907 3443
Fragments arranged by size:
3443 907 500
PasI CC'CwG_GG
Cuts at: 816 1462 816
Size: 646 4204
PciI A'CATG_T
Cuts at: 3306 3306
Size: 4850
PflMI CCAn_nnn'nTGG
Cuts at: 213 213
Size: 4850
PfoI T'CCnGG_A
Cuts at: 775 775
Size: 4850
PmeI GTTT'AAAC
Cuts at: 1849 1849
Size: 4850
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3989 4021 3989
Size: 32 4818
PpuMI rG'GwC_Cy
Cuts at: 166 166
Size: 4850
PshAI GACnn'nnGTC
Cuts at: 1277 1277
Size: 4850
PspOMI G'GGCC_C
Cuts at: 1614 1614
Size: 4850
PstI C_TGCA'G
Cuts at: 618 896 1298 1502 1582 1897 2582 618
Size: 278 402 204 80 315 685 2886
Fragments arranged by size:
2886 685 402 315 278 204 80
PvuI CG_AT'CG
Cuts at: 104 104
Size: 4850
PvuII CAG'CTG
Cuts at: 755 911 2275 2635 755
Size: 156 1364 360 2970
Fragments arranged by size:
2970 1364 360 156
RsrII CG'GwC_CG
Cuts at: 3045 3045
Size: 4850
SacI G_AGCT'C
Cuts at: 1864 1864
Size: 4850
SacII CC_GC'GG
Cuts at: 1441 1441
Size: 4850
SalI G'TCGA_C
Cuts at: 1887 1887
Size: 4850
SanDI GG'GwC_CC
Cuts at: 166 166
Size: 4850
SapI GCTCTTCn'nnn_
Cuts at: 2873 3083 2873
Size: 210 4640
SbfI CC_TGCA'GG
Cuts at: 1582 1897 1582
Size: 315 4535
ScaI AGT'ACT
Cuts at: 4367 4367
Size: 4850
SfcI C'TryA_G
Cuts at: 33 614 669 747 892 1294 1498 1578 1893
Size: 581 55 78 145 402 204 80 315
Cuts at: 1893 2578 3571 3762 33
Size: 685 993 191 1121
Fragments arranged by size:
1121 993 685 581 402 315 204 191
145 80 78 55
SfoI GGC'GCC
Cuts at: 1200 1556 2282 2530 1200
Size: 356 726 248 3520
Fragments arranged by size:
3520 726 356 248
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 4850
SmaI CCC'GGG
Cuts at: 1872 1872
Size: 4850
SmlI C'TyrA_G
Cuts at: 2005 2920 3412 3674 2005
Size: 915 492 262 3181
Fragments arranged by size:
3181 915 492 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 4850
SphI G_CATG'C
Cuts at: 1903 2403 1903
Size: 500 4350
StuI AGG'CCT
Cuts at: 741 741
Size: 4850
StyI C'CwwG_G
Cuts at: 1644 1785 1794 1984 2338 1644
Size: 141 9 190 354 4156
Fragments arranged by size:
4156 354 190 141 9
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 397 661 761 1529 2433 2566 3025 3234 397
Size: 264 100 768 904 133 459 209 2013
Fragments arranged by size:
2013 904 768 459 264 209 133 100
TatI w'GTAC_w
Cuts at: 4365 4365
Size: 4850
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 1226 1258 1671 1703 4367 4399 1226
Size: 32 413 32 2664 32 1677
Fragments arranged by size:
2664 1677 413 32 32 32
Tth111I GACn'n_nGTC
Cuts at: 2647 2647
Size: 4850
XbaI T'CTAG_A
Cuts at: 1353 1881 1353
Size: 528 4322
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 823 2345 823
Size: 1522 3328
XmaI C'CCGG_G
Cuts at: 1870 1870
Size: 4850
XmnI GAAnn'nnTTC
Cuts at: 1415 1415
Size: 4850
Enzymes that do cut:
AarI AccI Acc65I AclI AcuI AflIII AgeI AleI
AlfI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI
AvrII BamHI BanI BanII BbeI BbvCI BcgI BciVI
BclI BfrBI BglII BlpI Bme1580I BmgBI BmrI BplI
BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI
BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI
Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BssSI
BstAPI BstBI BstXI BstYI Bsu36I BtgI BtgZI BtsI
DraI DraIII DrdI EaeI EagI EarI EciI EcoICRI
Eco57MI EcoNI EcoO109I EcoRI FalI FspI HaeII Hin4I
HincII HindIII KasI KpnI MluI MmeI MscI MslI
MspA1I NaeI NarI NcoI NgoMIV NsiI NspI PasI
PciI PflMI PfoI PmeI PpiI PpuMI PshAI PspOMI
PstI PvuI PvuII RsrII SacI SacII SalI SanDI
SapI SbfI ScaI SfcI SfoI SgfI SmaI SmlI
SpeI SphI StuI StyI TaqII TatI TstI Tth111I
XbaI XcmI XmaI XmnI
Enzymes that do not cut:
AatII AfeI AflII AhdI AloI AscI BaeI BbsI
BglI BmtI BsiWI BsrGI BssHII BstEII BstZ17I ClaI
CspCI EcoRV FseI FspAI HpaI MfeI NdeI NheI
NotI NruI PacI PmlI PsiI PspXI PsrI SexAI
SfiI SgrAI SnaBI SrfI SspI SwaI XhoI ZraI