Restriction map for
pF1KE0067
(Circular) (Six-base) MAPSORT of: pF1KE0067.seq Check: 7054 from: 1 to: 7454
pF1KE0067 7454 bp
With 182 enzymes: SgfI *
April 24, 2009 13:10 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 1470 1470
Size: 7454
AatII G_ACGT'C
Cuts at: 2734 2734
Size: 7454
AccI GT'mk_AC
Cuts at: 1030 2239 4492 1030
Size: 1209 2253 3992
Fragments arranged by size:
3992 2253 1209
Acc65I G'GTAC_C
Cuts at: 135 2147 4470 135
Size: 2012 2323 3119
Fragments arranged by size:
3119 2323 2012
AclI AA'CG_TT
Cuts at: 7294 7294
Size: 7454
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1546 1603 3207 3467 4337 5279 5711 6458 6726
Size: 57 1604 260 870 942 432 747 268
Cuts at: 6726 1546
Size: 2274
Fragments arranged by size:
2274 1604 942 870 747 432 268 260
57
AflIII A'CryG_T
Cuts at: 4806 5910 6887 4806
Size: 1104 977 5373
Fragments arranged by size:
5373 1104 977
AgeI A'CCGG_T
Cuts at: 196 5842 196
Size: 5646 1808
AleI CACnn'nnGTG
Cuts at: 1807 1807
Size: 7454
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 4442 4442
Size: 7454
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2507 2539 4172 4204 2507
Size: 32 1633 32 5757
Fragments arranged by size:
5757 1633 32 32
AlwNI CAG_nnn'CTG
Cuts at: 550 1171 4315 4759 6326 550
Size: 621 3144 444 1567 1678
Fragments arranged by size:
3144 1678 1567 621 444
ApaI G_GGCC'C
Cuts at: 4223 4223
Size: 7454
ApaLI G'TGCA_C
Cuts at: 1343 6224 1343
Size: 4881 2573
ApoI r'AATT_y
Cuts at: 731 794 1876 2084 2857 3170 3183 4458 6807
Size: 63 1082 208 773 313 13 1275 2349
Cuts at: 6807 7063 731
Size: 256 1122
Fragments arranged by size:
2349 1275 1122 1082 773 313 256 208
63 13
AseI AT'TA_AT
Cuts at: 20 20
Size: 7454
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 7454
AvaI C'yCGr_G
Cuts at: 668 3797 4474 668
Size: 3129 677 3648
Fragments arranged by size:
3648 3129 677
BamHI G'GATC_C
Cuts at: 3569 4479 3569
Size: 910 6544
BanI G'GyrC_C
Cuts at: 135 1454 2147 3864 4083 4470 4884 5132 5167
Size: 1319 693 1717 219 387 414 248 35
Cuts at: 5167 135
Size: 2422
Fragments arranged by size:
2422 1717 1319 693 414 387 248 219
35
BanII G_rGCy'C
Cuts at: 608 1082 2407 3754 3795 4223 4468 5498 608
Size: 474 1325 1347 41 428 245 1030 2564
Fragments arranged by size:
2564 1347 1325 1030 474 428 245 41
BbeI G_GCGC'C
Cuts at: 1458 4888 5136 1458
Size: 3430 248 3776
Fragments arranged by size:
3776 3430 248
BbsI GAAGACnn'nnnn_
Cuts at: 1885 2807 1885
Size: 922 6532
BciVI GTATCCnnnnn_n'
Cuts at: 1259 5352 6113 7454 1259
Size: 4093 761 1341 1259
Fragments arranged by size:
4093 1341 1259 761
BclI T'GATC_A
Cuts at: 320 2286 3407 4974 320
Size: 1966 1121 1567 2800
Fragments arranged by size:
2800 1966 1567 1121
BglI GCCn_nnn'nGGC
Cuts at: 3799 3799
Size: 7454
BglII A'GATC_T
Cuts at: 2622 4969 7006 2622
Size: 2347 2037 3070
Fragments arranged by size:
3070 2347 2037
BlpI GC'TnA_GC
Cuts at: 744 4440 4566 744
Size: 3696 126 3632
Fragments arranged by size:
3696 3632 126
Bme1580I G_kGCm'C
Cuts at: 1187 1347 1980 3885 4223 5079 5172 6228 1187
Size: 160 633 1905 338 856 93 1056 2413
Fragments arranged by size:
2413 1905 1056 856 633 338 160 93
BmgBI CAC'GTC
Cuts at: 1968 1968
Size: 7454
BmrI ACTGGGnnnn_n'
Cuts at: 2569 3709 3822 3879 4666 4747 5082 7226 2569
Size: 1140 113 57 787 81 335 2144 2797
Fragments arranged by size:
2797 2144 1140 787 335 113 81 57
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2360 5821 2360
Size: 3461 3993
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 710 1365 1491 2149 2185 2237 3787 710
Size: 655 126 658 36 52 1550 4377
Fragments arranged by size:
4377 1550 658 655 126 52 36
Bpu10I CC'TnA_GC
Cuts at: 1361 4061 1361
Size: 2700 4754
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 568 750 2261 4630 5509 6001 6299 568
Size: 182 1511 2369 879 492 298 1723
Fragments arranged by size:
2369 1723 1511 879 492 298 182
BsaI GGTCTCn'nnnn_
Cuts at: 398 7158 398
Size: 6760 694
BsaAI yAC'GTr
Cuts at: 2924 4807 2924
Size: 1883 5571
BsaBI GATnn'nnATC
Cuts at: 6 2585 4968 7005 6
Size: 2579 2383 2037 455
Fragments arranged by size:
2579 2383 2037 455
BsaHI Gr'CG_yC
Cuts at: 1455 1928 2731 4885 5133 5835 7331 1455
Size: 473 803 2154 248 702 1496 1578
Fragments arranged by size:
2154 1578 1496 803 702 473 248
BsaWI w'CCGG_w
Cuts at: 196 2756 4643 5164 5842 6116 6263 6674 196
Size: 2560 1887 521 678 274 147 411 976
Fragments arranged by size:
2560 1887 976 678 521 411 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 260 290 302 332 560 590 1202 1232 2507
Size: 30 12 30 228 30 612 30 1275
Cuts at: 2507 2537 2931 2961 260
Size: 30 394 30 4753
Fragments arranged by size:
4753 1275 612 394 228 30 30 30
30 30 30 12
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 271 323 2033 2336 3371 4093 4342 271
Size: 52 1710 303 1035 722 249 3383
Fragments arranged by size:
3383 1710 1035 722 303 249 52
BseYI C'CCAG_C
Cuts at: 2696 3488 4879 5637 6214 6710 2696
Size: 792 1391 758 577 496 3440
Fragments arranged by size:
3440 1391 792 758 577 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1785 2894 3767 1785
Size: 1109 873 5472
Fragments arranged by size:
5472 1109 873
BsiEI CG_ry'CG
Cuts at: 104 306 4996 5042 6250 104
Size: 202 4690 46 1208 1308
Fragments arranged by size:
4690 1308 1208 202 46
BsiHKAI G_wGCw'C
Cuts at: 1082 1347 3754 4468 5246 5436 6228 1082
Size: 265 2407 714 778 190 792 2308
Fragments arranged by size:
2407 2308 792 778 714 265 190
BsmI GAATG_Cn'
Cuts at: 341 4065 341
Size: 3724 3730
BsmBI CGTCTCn'nnnn_
Cuts at: 1450 1935 1998 3911 1450
Size: 485 63 1913 4993
Fragments arranged by size:
4993 1913 485 63
Bsp1286I G_dGCh'C
Cuts at: 608 1082 1187 1347 1980 2407 3754 3795 3885
Size: 474 105 160 633 427 1347 41 90
Cuts at: 3885 4223 4468 5079 5172 5246 5436 5498 6228
Size: 338 245 611 93 74 190 62 730
Cuts at: 6228 608
Size: 1834
Fragments arranged by size:
1834 1347 730 633 611 474 427 338
245 190 160 105 93 90 74 62
41
BspEI T'CCGG_A
Cuts at: 6674 6674
Size: 7454
BspHI T'CATG_A
Cuts at: 2333 3576 3809 4412 6630 2333
Size: 1243 233 603 2218 3157
Fragments arranged by size:
3157 2218 1243 603 233
BspMI ACCTGCnnnn'nnnn_
Cuts at: 1470 1715 3407 4504 4718 5020 5401 1470
Size: 245 1692 1097 214 302 381 3523
Fragments arranged by size:
3523 1692 1097 381 302 245 214
BsrBI CCG'CTC
Cuts at: 50 5746 5800 7284 7449 50
Size: 5696 54 1484 165 55
Fragments arranged by size:
5696 1484 165 55 54
BsrDI GCAATG_nn'
Cuts at: 694 5366 694
Size: 4672 2782
BsrFI r'CCGG_y
Cuts at: 196 2074 5452 5633 5842 196
Size: 1878 3378 181 209 1808
Fragments arranged by size:
3378 1878 1808 209 181
BsrGI T'GTAC_A
Cuts at: 1162 1598 2655 1162
Size: 436 1057 5961
Fragments arranged by size:
5961 1057 436
BssHII G'CGCG_C
Cuts at: 179 179
Size: 7454
BssSI C'ACGA_G
Cuts at: 2987 5725 6083 2987
Size: 2738 358 4358
Fragments arranged by size:
4358 2738 358
BstAPI GCAn_nnn'nTGC
Cuts at: 508 4831 508
Size: 4323 3131
BstBI TT'CG_AA
Cuts at: 3168 5815 3168
Size: 2647 4807
BstEII G'GTnAC_C
Cuts at: 3417 3417
Size: 7454
BstXI CCAn_nnnn'nTGG
Cuts at: 1410 1662 3520 1410
Size: 252 1858 5344
Fragments arranged by size:
5344 1858 252
BstYI r'GATC_y
Cuts at: 1 804 2622 3138 3569 4279 4479 4946 4969
Size: 803 1818 516 431 710 200 467 23
Cuts at: 4969 5304 5550 6562 6648 6660 7006 1
Size: 335 246 1012 86 12 346 449
Fragments arranged by size:
1818 1012 803 710 516 467 449 431
346 335 246 200 86 23 12
BstZ17I GTA'TAC
Cuts at: 1031 2240 1031
Size: 1209 6245
BtgI C'CryG_G
Cuts at: 107 293 1099 3818 107
Size: 186 806 2719 3743
Fragments arranged by size:
3743 2719 806 186
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 5404 5585 5748 5404
Size: 181 163 7110
Fragments arranged by size:
7110 181 163
BtsI GCAGTG_nn'
Cuts at: 223 3378 4697 223
Size: 3155 1319 2980
Fragments arranged by size:
3155 2980 1319
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 2348 2383 3176 3211 2348
Size: 35 793 35 6591
Fragments arranged by size:
6591 793 35 35
DraI TTT'AAA
Cuts at: 1109 4453 1109
Size: 3344 4110
DrdI GACnn_nn'nnGTC
Cuts at: 2710 2962 5160 6018 2710
Size: 252 2198 858 4146
Fragments arranged by size:
4146 2198 858 252
EaeI y'GGCC_r
Cuts at: 1664 3295 5039 5213 5604 5631 6976 1664
Size: 1631 1744 174 391 27 1345 2142
Fragments arranged by size:
2142 1744 1631 1345 391 174 27
EagI C'GGCC_G
Cuts at: 5039 5039
Size: 7454
EarI CTCTTCn'nnn_
Cuts at: 163 2306 3226 3486 4232 5477 5687 163
Size: 2143 920 260 746 1245 210 1930
Fragments arranged by size:
2143 1930 1245 920 746 260 210
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 1085 5662 5972 6118 7264 1085
Size: 4577 310 146 1146 1275
Fragments arranged by size:
4577 1275 1146 310 146
EcoICRI GAG'CTC
Cuts at: 1080 3752 4466 1080
Size: 2672 714 4068
Fragments arranged by size:
4068 2672 714
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 710 1365 1491 1546 1603 2149 2185 2237 3207
Size: 655 126 55 57 546 36 52 970
Cuts at: 3207 3467 3787 4337 5279 5711 6458 6726 710
Size: 260 320 550 942 432 747 268 1438
Fragments arranged by size:
1438 970 942 747 655 550 546 432
320 268 260 126 57 55 52 36
EcoNI CCTnn'n_nnAGG
Cuts at: 2048 2048
Size: 7454
EcoO109I rG'GnC_Cy
Cuts at: 1556 2312 3546 4219 4593 1556
Size: 756 1234 673 374 4417
Fragments arranged by size:
4417 1234 756 673 374
EcoRI G'AATT_C
Cuts at: 2857 3170 4458 2857
Size: 313 1288 5853
Fragments arranged by size:
5853 1288 313
EcoRV GAT'ATC
Cuts at: 3922 3922
Size: 7454
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 4301 5473 4301
Size: 1172 6282
FspI TGC'GCA
Cuts at: 5235 5235
Size: 7454
HaeII r_GCGC'y
Cuts at: 1458 3857 4888 5136 6158 7137 1458
Size: 2399 1031 248 1022 979 1775
Fragments arranged by size:
2399 1775 1031 1022 979 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 260 292 466 498 662 694 1202 1234 1397
Size: 32 174 32 164 32 508 32 163
Cuts at: 1397 1429 1834 1866 2794 2826 3814 3846 5296
Size: 32 405 32 928 32 988 32 1450
Cuts at: 5296 5326 5328 5358 260
Size: 30 2 30 2356
Fragments arranged by size:
2356 1450 988 928 508 405 174 164
163 32 32 32 32 32 32 32
32 30 30 2
HincII GTy'rAC
Cuts at: 4493 4493
Size: 7454
HindIII A'AGCT_T
Cuts at: 212 2498 212
Size: 2286 5168
KasI G'GCGC_C
Cuts at: 1454 4884 5132 1454
Size: 3430 248 3776
Fragments arranged by size:
3776 3430 248
KpnI G_GTAC'C
Cuts at: 139 2151 4474 139
Size: 2012 2323 3119
Fragments arranged by size:
3119 2323 2012
MfeI C'AATT_G
Cuts at: 3080 3344 3080
Size: 264 7190
MluI A'CGCG_T
Cuts at: 6887 6887
Size: 7454
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 326 475 738 972 1145 2255 2320 3576 3857
Size: 149 263 234 173 1110 65 1256 281
Cuts at: 3857 6125 6309 326
Size: 2268 184 1471
Fragments arranged by size:
2268 1471 1256 1110 281 263 234 184
173 149 65
MscI TGG'CCA
Cuts at: 1666 3297 5215 1666
Size: 1631 1918 3905
Fragments arranged by size:
3905 1918 1631
MslI CAynn'nnrTG
Cuts at: 1660 1807 2332 2368 2635 3281 3371 3436 3862
Size: 147 525 36 267 646 90 65 426
Cuts at: 3862 5443 5570 1660
Size: 1581 127 3544
Fragments arranged by size:
3544 1581 646 525 426 267 147 127
90 65 36
MspA1I CmG'CkG
Cuts at: 295 825 1451 2696 4565 4879 5239 6252 6497
Size: 530 626 1245 1869 314 360 1013 245
Cuts at: 6497 295
Size: 1252
Fragments arranged by size:
1869 1252 1245 1013 626 530 360 314
245
NaeI GCC'GGC
Cuts at: 5635 5635
Size: 7454
NarI GG'CG_CC
Cuts at: 1455 4885 5133 1455
Size: 3430 248 3776
Fragments arranged by size:
3776 3430 248
NcoI C'CATG_G
Cuts at: 107 1099 3818 107
Size: 992 2719 3743
Fragments arranged by size:
3743 2719 992
NgoMIV G'CCGG_C
Cuts at: 5633 5633
Size: 7454
NspI r_CATG'y
Cuts at: 2917 4507 5007 5914 2917
Size: 1590 500 907 4457
Fragments arranged by size:
4457 1590 907 500
PciI A'CATG_T
Cuts at: 5910 5910
Size: 7454
PflMI CCAn_nnn'nTGG
Cuts at: 2897 2897
Size: 7454
PfoI T'CCnGG_A
Cuts at: 807 881 807
Size: 74 7380
PmeI GTTT'AAAC
Cuts at: 4453 4453
Size: 7454
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2507 2539 6593 6625 2507
Size: 32 4054 32 3336
Fragments arranged by size:
4054 3336 32 32
PpuMI rG'GwC_Cy
Cuts at: 1556 3546 1556
Size: 1990 5464
PshAI GACnn'nnGTC
Cuts at: 3269 3269
Size: 7454
PsiI TTA'TAA
Cuts at: 1856 1910 1856
Size: 54 7400
PspOMI G'GGCC_C
Cuts at: 4219 4219
Size: 7454
PstI C_TGCA'G
Cuts at: 1077 1467 2247 3630 4501 5186 1077
Size: 390 780 1383 871 685 3345
Fragments arranged by size:
3345 1383 871 780 685 390
PvuI CG_AT'CG
Cuts at: 104 104
Size: 7454
PvuII CAG'CTG
Cuts at: 825 1451 2696 4879 5239 825
Size: 626 1245 2183 360 3040
Fragments arranged by size:
3040 2183 1245 626 360
RsrII CG'GwC_CG
Cuts at: 5649 5649
Size: 7454
SacI G_AGCT'C
Cuts at: 1082 3754 4468 1082
Size: 2672 714 4068
Fragments arranged by size:
4068 2672 714
SacII CC_GC'GG
Cuts at: 296 296
Size: 7454
SalI G'TCGA_C
Cuts at: 4491 4491
Size: 7454
SanDI GG'GwC_CC
Cuts at: 1556 1556
Size: 7454
SapI GCTCTTCn'nnn_
Cuts at: 3486 5477 5687 3486
Size: 1991 210 5253
Fragments arranged by size:
5253 1991 210
SbfI CC_TGCA'GG
Cuts at: 3630 4501 3630
Size: 871 6583
ScaI AGT'ACT
Cuts at: 2895 6971 2895
Size: 4076 3378
SexAI A'CCwGG_T
Cuts at: 922 2548 922
Size: 1626 5828
SfcI C'TryA_G
Cuts at: 33 1073 1463 2243 3626 4497 5182 6175 6366
Size: 1040 390 780 1383 871 685 993 191
Cuts at: 6366 33
Size: 1121
Fragments arranged by size:
1383 1121 1040 993 871 780 685 390
191
SfoI GGC'GCC
Cuts at: 1456 4886 5134 1456
Size: 3430 248 3776
Fragments arranged by size:
3776 3430 248
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 7454
SmaI CCC'GGG
Cuts at: 3799 4476 3799
Size: 677 6777
SmlI C'TyrA_G
Cuts at: 583 765 2276 4609 5524 6016 6278 583
Size: 182 1511 2333 915 492 262 1759
Fragments arranged by size:
2333 1759 1511 915 492 262 182
SpeI A'CTAG_T
Cuts at: 67 67
Size: 7454
SphI G_CATG'C
Cuts at: 4507 5007 4507
Size: 500 6954
SspI AAT'ATT
Cuts at: 1062 3600 1062
Size: 2538 4916
StuI AGG'CCT
Cuts at: 1868 2834 1868
Size: 966 6488
StyI C'CwwG_G
Cuts at: 107 353 1099 1782 2951 3017 3062 3267 3818
Size: 246 746 683 1169 66 45 205 551
Cuts at: 3818 4257 4588 4942 107
Size: 439 331 354 2619
Fragments arranged by size:
2619 1169 746 683 551 439 354 331
246 205 66 45
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 320 967 2604 2853 3526 3992 4142 5037 5170
Size: 647 1637 249 673 466 150 895 133
Cuts at: 5170 5629 5838 320
Size: 459 209 1936
Fragments arranged by size:
1936 1637 895 673 647 466 459 249
209 150 133
TatI w'GTAC_w
Cuts at: 153 1162 1598 2180 2655 2893 6969 153
Size: 1009 436 582 475 238 4076 638
Fragments arranged by size:
4076 1009 638 582 475 436 238
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 215 247 302 334 515 547 560 592 1166
Size: 32 55 32 181 32 13 32 574
Cuts at: 1166 1198 2931 2963 2973 3005 6971 7003 215
Size: 32 1733 32 10 32 3966 32 666
Fragments arranged by size:
3966 1733 666 574 181 55 32 32
32 32 32 32 32 32 13 10
Tth111I GACn'n_nGTC
Cuts at: 5251 5251
Size: 7454
XbaI T'CTAG_A
Cuts at: 2265 4485 2265
Size: 2220 5234
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 323 620 920 2141 2231 3086 3274 4949 323
Size: 297 300 1221 90 855 188 1675 2828
Fragments arranged by size:
2828 1675 1221 855 300 297 188 90
XmaI C'CCGG_G
Cuts at: 3797 4474 3797
Size: 677 6777
XmnI GAAnn'nnTTC
Cuts at: 1876 2522 1876
Size: 646 6808
ZraI GAC'GTC
Cuts at: 2732 2732
Size: 7454
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AflIII AgeI
AleI AlfI AloI AlwNI ApaI ApaLI ApoI AseI
AsiSI AvaI BamHI BanI BanII BbeI BbsI BciVI
BclI BglI BglII BlpI Bme1580I BmgBI BmrI BplI
BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI
BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI
Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI
BssHII BssSI BstAPI BstBI BstEII BstXI BstYI BstZ17I
BtgI BtgZI BtsI CspCI DraI DrdI EaeI EagI
EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI EcoRV
FalI FspI HaeII Hin4I HincII HindIII KasI KpnI
MfeI MluI MmeI MscI MslI MspA1I NaeI NarI
NcoI NgoMIV NspI PciI PflMI PfoI PmeI PpiI
PpuMI PshAI PsiI PspOMI PstI PvuI PvuII RsrII
SacI SacII SalI SanDI SapI SbfI ScaI SexAI
SfcI SfoI SgfI SmaI SmlI SpeI SphI SspI
StuI StyI TaqII TatI TstI Tth111I XbaI XcmI
XmaI XmnI ZraI
Enzymes that do not cut:
AfeI AflII AhdI AscI AvrII BaeI BbvCI BcgI
BfrBI BmtI BsiWI Bsu36I ClaI DraIII FseI FspAI
HpaI NdeI NheI NotI NruI NsiI PacI PasI
PmlI PspXI PsrI SfiI SgrAI SnaBI SrfI SwaI
XhoI