Restriction map for
pF1KE0073
(Circular) (Six-base) MAPSORT of: pF1KE0073.seq Check: 238 from: 1 to: 7073
pF1KE0073 7073 bp
With 182 enzymes: SgfI *
April 24, 2009 13:10 ..
AccI GT'mk_AC
Cuts at: 791 4111 791
Size: 3320 3753
Acc65I G'GTAC_C
Cuts at: 4089 4089
Size: 7073
AclI AA'CG_TT
Cuts at: 6913 6913
Size: 7073
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 3559 3658 4898 5330 6077 6345 3559
Size: 99 1240 432 747 268 4287
Fragments arranged by size:
4287 1240 747 432 268 99
AfeI AGC'GCT
Cuts at: 2277 3780 2277
Size: 1503 5570
AflIII A'CryG_T
Cuts at: 842 4425 5529 6506 842
Size: 3583 1104 977 1409
Fragments arranged by size:
3583 1409 1104 977
AgeI A'CCGG_T
Cuts at: 5461 5461
Size: 7073
AhdI GACnn_n'nnGTC
Cuts at: 1665 3282 1665
Size: 1617 5456
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 3797 3955 3797
Size: 158 6915
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2548 2580 2548
Size: 32 7041
AlwNI CAG_nnn'CTG
Cuts at: 1431 1671 1815 3630 3713 3948 4378 5945 1431
Size: 240 144 1815 83 235 430 1567 2559
Fragments arranged by size:
2559 1815 1567 430 240 235 144 83
ApaI G_GGCC'C
Cuts at: 239 2143 2631 2769 3478 239
Size: 1904 488 138 709 3834
Fragments arranged by size:
3834 1904 709 488 138
ApaLI G'TGCA_C
Cuts at: 370 5843 370
Size: 5473 1600
ApoI r'AATT_y
Cuts at: 1221 4077 6426 6682 1221
Size: 2856 2349 256 1612
Fragments arranged by size:
2856 2349 1612 256
AseI AT'TA_AT
Cuts at: 20 20
Size: 7073
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 7073
AvaI C'yCGr_G
Cuts at: 411 475 813 828 918 1201 1849 2055 2121
Size: 64 338 15 90 283 648 206 66
Cuts at: 2121 2251 2326 2623 2727 2817 3477 3989 4093
Size: 130 75 297 104 90 660 512 104
Cuts at: 4093 411
Size: 3391
Fragments arranged by size:
3391 660 648 512 338 297 283 206
130 104 104 90 90 75 66 64
15
BamHI G'GATC_C
Cuts at: 252 4098 252
Size: 3846 3227
BanI G'GyrC_C
Cuts at: 646 822 922 1109 1416 1939 1954 1971 2097
Size: 176 100 187 307 523 15 17 126
Cuts at: 2097 2455 2851 2974 3096 3376 3399 3535 3843
Size: 358 396 123 122 280 23 136 308
Cuts at: 3843 4089 4503 4751 4786 646
Size: 246 414 248 35 2933
Fragments arranged by size:
2933 523 414 396 358 308 307 280
248 246 187 176 136 126 123 122
100 35 23 17 15
BanII G_rGCy'C
Cuts at: 134 239 308 697 1094 1105 2143 2270 2533
Size: 105 69 389 397 11 1038 127 263
Cuts at: 2533 2631 2734 2769 2971 3034 3478 4087 5117
Size: 98 103 35 202 63 444 609 1030
Cuts at: 5117 134
Size: 2090
Fragments arranged by size:
2090 1038 1030 609 444 397 389 263
202 127 105 103 98 69 63 35
11
BbeI G_GCGC'C
Cuts at: 826 926 1420 1943 1975 2101 3380 3403 3539
Size: 100 494 523 32 126 1279 23 136
Cuts at: 3539 3847 4507 4755 826
Size: 308 660 248 3144
Fragments arranged by size:
3144 1279 660 523 494 308 248 136
126 100 32 23
BbvCI CC'TCA_GC
Cuts at: 1151 3915 1151
Size: 2764 4309
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 481 515 2145 2179 3883 3917 481
Size: 34 1630 34 1704 34 3637
Fragments arranged by size:
3637 1704 1630 34 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 1343 4971 5732 7073 1343
Size: 3628 761 1341 1343
Fragments arranged by size:
3628 1343 1341 761
BclI T'GATC_A
Cuts at: 4593 4593
Size: 7073
BglI GCCn_nnn'nGGC
Cuts at: 509 667 1203 1592 1868 1892 2339 3488 3633
Size: 158 536 389 276 24 447 1149 145
Cuts at: 3633 3670 3679 4008 509
Size: 37 9 329 3574
Fragments arranged by size:
3574 1149 536 447 389 329 276 158
145 37 24 9
BglII A'GATC_T
Cuts at: 4588 6625 4588
Size: 2037 5036
BlpI GC'TnA_GC
Cuts at: 1835 4185 1835
Size: 2350 4723
Bme1580I G_kGCm'C
Cuts at: 239 374 1231 1270 1616 1957 2143 2186 2631
Size: 135 857 39 346 341 186 43 445
Cuts at: 2631 2769 3099 3478 3493 4004 4698 4791 5847
Size: 138 330 379 15 511 694 93 1056
Cuts at: 5847 239
Size: 1465
Fragments arranged by size:
1465 1056 857 694 511 445 379 346
341 330 186 138 135 93 43 39
15
BmgBI CAC'GTC
Cuts at: 1857 2888 3125 3972 1857
Size: 1031 237 847 4958
Fragments arranged by size:
4958 1031 847 237
BmrI ACTGGGnnnn_n'
Cuts at: 239 4285 4366 4701 6845 239
Size: 4046 81 335 2144 467
Fragments arranged by size:
4046 2144 467 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 843 3258 5440 843
Size: 2415 2182 2476
Fragments arranged by size:
2476 2415 2182
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 636 717 778 1089 1108 1369 2819 3523 3586
Size: 81 61 311 19 261 1450 704 63
Cuts at: 3586 636
Size: 4123
Fragments arranged by size:
4123 1450 704 311 261 81 63 61
19
Bpu10I CC'TnA_GC
Cuts at: 1151 3915 1151
Size: 2764 4309
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 4249 5128 5620 5918 4249
Size: 879 492 298 5404
Fragments arranged by size:
5404 879 492 298
BsaI GGTCTCn'nnnn_
Cuts at: 175 2021 2692 2916 6777 175
Size: 1846 671 224 3861 471
Fragments arranged by size:
3861 1846 671 471 224
BsaAI yAC'GTr
Cuts at: 2181 2964 4426 2181
Size: 783 1462 4828
Fragments arranged by size:
4828 1462 783
BsaBI GATnn'nnATC
Cuts at: 6 4587 6624 6
Size: 4581 2037 455
Fragments arranged by size:
4581 2037 455
BsaHI Gr'CG_yC
Cuts at: 823 923 1417 1792 1940 1947 1972 2098 3103
Size: 100 494 375 148 7 25 126 1005
Cuts at: 3103 3377 3400 3536 3844 3980 4504 4752 5454
Size: 274 23 136 308 136 524 248 702
Cuts at: 5454 6950 823
Size: 1496 946
Fragments arranged by size:
1496 1005 946 702 524 494 375 308
274 248 148 136 136 126 100 25
23 7
BsaWI w'CCGG_w
Cuts at: 184 1355 3003 4262 4783 5461 5735 5882 6293
Size: 1171 1648 1259 521 678 274 147 411
Cuts at: 6293 184
Size: 964
Fragments arranged by size:
1648 1259 1171 964 678 521 411 274
147
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1394 2096 1394
Size: 702 6371
BseYI C'CCAG_C
Cuts at: 270 977 998 1016 1130 1250 1273 1309 1904
Size: 707 21 18 114 120 23 36 595
Cuts at: 1904 2089 2272 2407 2485 2526 2545 3702 3923
Size: 185 183 135 78 41 19 1157 221
Cuts at: 3923 4498 5256 5833 6329 270
Size: 575 758 577 496 1014
Fragments arranged by size:
1157 1014 758 707 595 577 575 496
221 185 183 135 120 114 78 41
36 23 21 19 18
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 475 2375 3945 475
Size: 1900 1570 3603
Fragments arranged by size:
3603 1900 1570
BsiEI CG_ry'CG
Cuts at: 104 616 2364 2928 4615 4661 5869 104
Size: 512 1748 564 1687 46 1208 1308
Fragments arranged by size:
1748 1687 1308 1208 564 512 46
BsiHKAI G_wGCw'C
Cuts at: 374 572 3259 3745 3776 4087 4865 5055 5847
Size: 198 2687 486 31 311 778 190 792
Cuts at: 5847 374
Size: 1600
Fragments arranged by size:
2687 1600 792 778 486 311 198 190
31
BsmI GAATG_Cn'
Cuts at: 4020 4020
Size: 7073
BsmBI CGTCTCn'nnnn_
Cuts at: 3079 3079
Size: 7073
Bsp1286I G_dGCh'C
Cuts at: 134 239 308 374 572 697 1094 1105 1231
Size: 105 69 66 198 125 397 11 126
Cuts at: 1231 1270 1616 1957 2143 2186 2270 2533 2631
Size: 39 346 341 186 43 84 263 98
Cuts at: 2631 2734 2769 2971 3034 3099 3259 3478 3493
Size: 103 35 202 63 65 160 219 15
Cuts at: 3493 3745 3776 4004 4087 4698 4791 4865 5055
Size: 252 31 228 83 611 93 74 190
Cuts at: 5055 5117 5847 134
Size: 62 730 1360
Fragments arranged by size:
1360 730 611 397 346 341 263 252
228 219 202 198 190 186 160 126
125 105 103 98 93 84 83 74
69 66 65 63 62 43 39 35
31 15 11
BspEI T'CCGG_A
Cuts at: 3003 6293 3003
Size: 3290 3783
BspHI T'CATG_A
Cuts at: 1775 6249 1775
Size: 4474 2599
BspMI ACCTGCnnnn'nnnn_
Cuts at: 2039 3498 4123 4337 4639 5020 2039
Size: 1459 625 214 302 381 4092
Fragments arranged by size:
4092 1459 625 381 302 214
BsrBI CCG'CTC
Cuts at: 50 5365 5419 6903 7068 50
Size: 5315 54 1484 165 55
Fragments arranged by size:
5315 1484 165 55 54
BsrDI GCAATG_nn'
Cuts at: 4985 4985
Size: 7073
BsrFI r'CCGG_y
Cuts at: 666 1343 2291 2359 2363 2719 2907 3343 5071
Size: 677 948 68 4 356 188 436 1728
Cuts at: 5071 5252 5461 666
Size: 181 209 2278
Fragments arranged by size:
2278 1728 948 677 436 356 209 188
181 68 4
BssHII G'CGCG_C
Cuts at: 266 322 2516 266
Size: 56 2194 4823
Fragments arranged by size:
4823 2194 56
BssSI C'ACGA_G
Cuts at: 483 3258 5344 5702 483
Size: 2775 2086 358 1854
Fragments arranged by size:
2775 2086 1854 358
BstAPI GCAn_nnn'nTGC
Cuts at: 369 1815 3713 4450 369
Size: 1446 1898 737 2992
Fragments arranged by size:
2992 1898 1446 737
BstBI TT'CG_AA
Cuts at: 5434 5434
Size: 7073
BstEII G'GTnAC_C
Cuts at: 3339 3339
Size: 7073
BstXI CCAn_nnnn'nTGG
Cuts at: 904 2511 3753 904
Size: 1607 1242 4224
Fragments arranged by size:
4224 1607 1242
BstYI r'GATC_y
Cuts at: 1 252 3554 3569 3584 4098 4565 4588 4923
Size: 251 3302 15 15 514 467 23 335
Cuts at: 4923 5169 6181 6267 6279 6625 1
Size: 246 1012 86 12 346 449
Fragments arranged by size:
3302 1012 514 467 449 346 335 251
246 86 23 15 15 12
Bsu36I CC'TnA_GG
Cuts at: 3675 3675
Size: 7073
BtgI C'CryG_G
Cuts at: 402 717 763 3366 402
Size: 315 46 2603 4109
Fragments arranged by size:
4109 2603 315 46
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 5023 5204 5367 5023
Size: 181 163 6729
Fragments arranged by size:
6729 181 163
BtsI GCAGTG_nn'
Cuts at: 2720 3949 3957 4316 2720
Size: 1229 8 359 5477
Fragments arranged by size:
5477 1229 359 8
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 444 479 444
Size: 35 7038
DraI TTT'AAA
Cuts at: 4072 4072
Size: 7073
DraIII CAC_nnn'GTG
Cuts at: 663 663
Size: 7073
DrdI GACnn_nn'nnGTC
Cuts at: 4779 5637 4779
Size: 858 6215
EaeI y'GGCC_r
Cuts at: 156 327 387 405 929 1466 2361 2501 2568
Size: 171 60 18 524 537 895 140 67
Cuts at: 2568 2717 2925 4658 4832 5223 5250 6595 156
Size: 149 208 1733 174 391 27 1345 634
Fragments arranged by size:
1733 1345 895 634 537 524 391 208
174 171 149 140 67 60 27 18
EagI C'GGCC_G
Cuts at: 2361 2925 4658 2361
Size: 564 1733 4776
Fragments arranged by size:
4776 1733 564
EarI CTCTTCn'nnn_
Cuts at: 5096 5306 5096
Size: 210 6863
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 592 1563 2006 2099 5281 5591 5737 6883 592
Size: 971 443 93 3182 310 146 1146 782
Fragments arranged by size:
3182 1146 971 782 443 310 146 93
EcoICRI GAG'CTC
Cuts at: 4085 4085
Size: 7073
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 636 717 778 1089 1108 1369 2819 3523 3559
Size: 81 61 311 19 261 1450 704 36
Cuts at: 3559 3586 3658 4898 5330 6077 6345 636
Size: 27 72 1240 432 747 268 1364
Fragments arranged by size:
1450 1364 1240 747 704 432 311 268
261 81 72 61 36 27 19
EcoNI CCTnn'n_nnAGG
Cuts at: 3505 3505
Size: 7073
EcoO109I rG'GnC_Cy
Cuts at: 213 235 236 479 589 1137 1297 2139 2140
Size: 22 1 243 110 548 160 842 1
Cuts at: 2140 2627 2628 2765 2766 2773 3185 3474 3671
Size: 487 1 137 1 7 412 289 197
Cuts at: 3671 3807 4212 213
Size: 136 405 3074
Fragments arranged by size:
3074 842 548 487 412 405 289 243
197 160 137 136 110 22 7 1
1 1 1
EcoRI G'AATT_C
Cuts at: 4077 4077
Size: 7073
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 5092 5092
Size: 7073
FspI TGC'GCA
Cuts at: 4854 4854
Size: 7073
HaeII r_GCGC'y
Cuts at: 826 914 926 1420 1550 1761 1943 1975 2101
Size: 88 12 494 130 211 182 32 126
Cuts at: 2101 2279 2292 3121 3380 3403 3539 3737 3782
Size: 178 13 829 259 23 136 198 45
Cuts at: 3782 3847 4507 4755 5777 6756 826
Size: 65 660 248 1022 979 1143
Fragments arranged by size:
1143 1022 979 829 660 494 259 248
211 198 182 178 136 130 126 88
65 45 32 23 13 12
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1651 1683 2977 3009 3268 3271 3300 3303 3315
Size: 32 1294 32 259 3 29 3 12
Cuts at: 3315 3347 3742 3774 4915 4945 4947 4977 1651
Size: 32 395 32 1141 30 2 30 3747
Fragments arranged by size:
3747 1294 1141 395 259 32 32 32
32 30 30 29 12 3 3 2
HincII GTy'rAC
Cuts at: 141 792 4112 141
Size: 651 3320 3102
Fragments arranged by size:
3320 3102 651
KasI G'GCGC_C
Cuts at: 822 922 1416 1939 1971 2097 3376 3399 3535
Size: 100 494 523 32 126 1279 23 136
Cuts at: 3535 3843 4503 4751 822
Size: 308 660 248 3144
Fragments arranged by size:
3144 1279 660 523 494 308 248 136
126 100 32 23
KpnI G_GTAC'C
Cuts at: 4093 4093
Size: 7073
MluI A'CGCG_T
Cuts at: 6506 6506
Size: 7073
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1239 3188 4058 5744 5928 1239
Size: 1949 870 1686 184 2384
Fragments arranged by size:
2384 1949 1686 870 184
MscI TGG'CCA
Cuts at: 158 1468 4834 158
Size: 1310 3366 2397
Fragments arranged by size:
3366 2397 1310
MslI CAynn'nnrTG
Cuts at: 298 1462 5062 5189 298
Size: 1164 3600 127 2182
Fragments arranged by size:
3600 2182 1164 127
MspA1I CmG'CkG
Cuts at: 684 737 873 889 1277 1428 1514 1812 2068
Size: 53 136 16 388 151 86 298 256
Cuts at: 2068 2241 2384 2804 3011 3533 3710 3799 4184
Size: 173 143 420 207 522 177 89 385
Cuts at: 4184 4498 4858 5871 6116 684
Size: 314 360 1013 245 1641
Fragments arranged by size:
1641 1013 522 420 388 385 360 314
298 256 245 207 177 173 151 143
136 89 86 53 16
NaeI GCC'GGC
Cuts at: 668 2293 2361 2365 2721 5254 668
Size: 1625 68 4 356 2533 2487
Fragments arranged by size:
2533 2487 1625 356 68 4
NarI GG'CG_CC
Cuts at: 823 923 1417 1940 1972 2098 3377 3400 3536
Size: 100 494 523 32 126 1279 23 136
Cuts at: 3536 3844 4504 4752 823
Size: 308 660 248 3144
Fragments arranged by size:
3144 1279 660 523 494 308 248 136
126 100 32 23
NgoMIV G'CCGG_C
Cuts at: 666 2291 2359 2363 2719 5252 666
Size: 1625 68 4 356 2533 2487
Fragments arranged by size:
2533 2487 1625 356 68 4
NspI r_CATG'y
Cuts at: 846 1365 1395 1539 3795 4126 4626 5533 846
Size: 519 30 144 2256 331 500 907 2386
Fragments arranged by size:
2386 2256 907 519 500 331 144 30
PasI CC'CwG_GG
Cuts at: 2841 3034 3848 2841
Size: 193 814 6066
Fragments arranged by size:
6066 814 193
PciI A'CATG_T
Cuts at: 842 5529 842
Size: 4687 2386
PflMI CCAn_nnn'nTGG
Cuts at: 756 1347 3564 3630 756
Size: 591 2217 66 4199
Fragments arranged by size:
4199 2217 591 66
PfoI T'CCnGG_A
Cuts at: 626 2788 626
Size: 2162 4911
PmeI GTTT'AAAC
Cuts at: 4072 4072
Size: 7073
PmlI CAC'GTG
Cuts at: 2181 2964 2181
Size: 783 6290
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 6212 6244 6212
Size: 32 7041
PpuMI rG'GwC_Cy
Cuts at: 2773 3185 2773
Size: 412 6661
PshAI GACnn'nnGTC
Cuts at: 2957 2957
Size: 7073
PspOMI G'GGCC_C
Cuts at: 235 2139 2627 2765 3474 235
Size: 1904 488 138 709 3834
Fragments arranged by size:
3834 1904 709 488 138
PspXI vC'TCGA_Gb
Cuts at: 3989 3989
Size: 7073
PstI C_TGCA'G
Cuts at: 1289 1436 1646 2150 2210 2246 4120 4805 1289
Size: 147 210 504 60 36 1874 685 3557
Fragments arranged by size:
3557 1874 685 504 210 147 60 36
PvuI CG_AT'CG
Cuts at: 104 104
Size: 7073
PvuII CAG'CTG
Cuts at: 873 2241 3710 4498 4858 873
Size: 1368 1469 788 360 3088
Fragments arranged by size:
3088 1469 1368 788 360
RsrII CG'GwC_CG
Cuts at: 3391 5268 3391
Size: 1877 5196
SacI G_AGCT'C
Cuts at: 4087 4087
Size: 7073
SalI G'TCGA_C
Cuts at: 790 4110 790
Size: 3320 3753
SanDI GG'GwC_CC
Cuts at: 3185 3185
Size: 7073
SapI GCTCTTCn'nnn_
Cuts at: 5096 5306 5096
Size: 210 6863
SbfI CC_TGCA'GG
Cuts at: 1289 4120 1289
Size: 2831 4242
ScaI AGT'ACT
Cuts at: 2992 6590 2992
Size: 3598 3475
SfcI C'TryA_G
Cuts at: 33 1285 1432 1642 2146 2206 2242 3592 4116
Size: 1252 147 210 504 60 36 1350 524
Cuts at: 4116 4801 5794 5985 33
Size: 685 993 191 1121
Fragments arranged by size:
1350 1252 1121 993 685 524 504 210
191 147 60 36
SfiI GGCCn_nnn'nGGCC
Cuts at: 1868 1868
Size: 7073
SfoI GGC'GCC
Cuts at: 824 924 1418 1941 1973 2099 3378 3401 3537
Size: 100 494 523 32 126 1279 23 136
Cuts at: 3537 3845 4505 4753 824
Size: 308 660 248 3144
Fragments arranged by size:
3144 1279 660 523 494 308 248 136
126 100 32 23
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 7073
SmaI CCC'GGG
Cuts at: 413 477 815 920 1203 1851 2253 2729 3479
Size: 64 338 105 283 648 402 476 750
Cuts at: 3479 4095 413
Size: 616 3391
Fragments arranged by size:
3391 750 648 616 476 402 338 283
105 64
SmlI C'TyrA_G
Cuts at: 3989 4228 5143 5635 5897 3989
Size: 239 915 492 262 5165
Fragments arranged by size:
5165 915 492 262 239
SpeI A'CTAG_T
Cuts at: 67 3312 67
Size: 3245 3828
SphI G_CATG'C
Cuts at: 1365 1395 1539 4126 4626 1365
Size: 30 144 2587 500 3812
Fragments arranged by size:
3812 2587 500 144 30
SrfI GCCC'GGGC
Cuts at: 3479 3479
Size: 7073
StuI AGG'CCT
Cuts at: 533 1586 2231 3913 533
Size: 1053 645 1682 3693
Fragments arranged by size:
3693 1682 1053 645
StyI C'CwwG_G
Cuts at: 780 2552 3983 4207 4561 780
Size: 1772 1431 224 354 3292
Fragments arranged by size:
3292 1772 1431 354 224
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 217 2716 3220 3408 3964 4656 4789 5248 5457
Size: 2499 504 188 556 692 133 459 209
Cuts at: 5457 217
Size: 1833
Fragments arranged by size:
2499 1833 692 556 504 459 209 188
133
TatI w'GTAC_w
Cuts at: 2990 6588 2990
Size: 3598 3475
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 1357 1389 6590 6622 1357
Size: 32 5201 32 1808
Fragments arranged by size:
5201 1808 32 32
Tth111I GACn'n_nGTC
Cuts at: 2936 4870 2936
Size: 1934 5139
XbaI T'CTAG_A
Cuts at: 4104 4104
Size: 7073
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 114 1344 2874 4568 114
Size: 1230 1530 1694 2619
Fragments arranged by size:
2619 1694 1530 1230
XhoI C'TCGA_G
Cuts at: 3989 3989
Size: 7073
XmaI C'CCGG_G
Cuts at: 411 475 813 918 1201 1849 2251 2727 3477
Size: 64 338 105 283 648 402 476 750
Cuts at: 3477 4093 411
Size: 616 3391
Fragments arranged by size:
3391 750 648 616 476 402 338 283
105 64
Enzymes that do cut:
AccI Acc65I AclI AcuI AfeI AflIII AgeI AhdI
AlfI AloI AlwNI ApaI ApaLI ApoI AseI AsiSI
AvaI BamHI BanI BanII BbeI BbvCI BcgI BciVI
BclI BglI BglII BlpI Bme1580I BmgBI BmrI BplI
BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI
BseRI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I
BspEI BspHI BspMI BsrBI BsrDI BsrFI BssHII BssSI
BstAPI BstBI BstEII BstXI BstYI Bsu36I BtgI BtgZI
BtsI CspCI DraI DraIII DrdI EaeI EagI EarI
EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI FalI FspI
HaeII Hin4I HincII KasI KpnI MluI MmeI MscI
MslI MspA1I NaeI NarI NgoMIV NspI PasI PciI
PflMI PfoI PmeI PmlI PpiI PpuMI PshAI PspOMI
PspXI PstI PvuI PvuII RsrII SacI SalI SanDI
SapI SbfI ScaI SfcI SfiI SfoI SgfI SmaI
SmlI SpeI SphI SrfI StuI StyI TaqII TatI
TstI Tth111I XbaI XcmI XhoI XmaI
Enzymes that do not cut:
AarI AatII AflII AleI AscI AvrII BaeI BbsI
BfrBI BmtI BsaXI BsiWI BsrGI BstZ17I ClaI EcoRV
FseI FspAI HindIII HpaI MfeI NcoI NdeI NheI
NotI NruI NsiI PacI PsiI PsrI SacII SexAI
SgrAI SnaBI SspI SwaI XmnI ZraI