Restriction map for
pF1KE0075
(Circular) (Six-base) MAPSORT of: pF1KE0075.seq Check: 3238 from: 1 to: 7454
pF1KE0075 7454 bp
With 182 enzymes: SgfI *
April 24, 2009 13:10 ..
AatII G_ACGT'C
Cuts at: 1418 1418
Size: 7454
AccI GT'mk_AC
Cuts at: 677 4492 677
Size: 3815 3639
Acc65I G'GTAC_C
Cuts at: 4470 4470
Size: 7454
AclI AA'CG_TT
Cuts at: 4448 7294 4448
Size: 2846 4608
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1656 1750 2634 2647 2656 4109 5279 5711 6458
Size: 94 884 13 9 1453 1170 432 747
Cuts at: 6458 6726 1656
Size: 268 2384
Fragments arranged by size:
2384 1453 1170 884 747 432 268 94
13 9
AfeI AGC'GCT
Cuts at: 629 2859 629
Size: 2230 5224
AflIII A'CryG_T
Cuts at: 680 2126 2513 3470 4806 5910 6887 680
Size: 1446 387 957 1336 1104 977 1247
Fragments arranged by size:
1446 1336 1247 1104 977 957 387
AgeI A'CCGG_T
Cuts at: 5842 5842
Size: 7454
AhdI GACnn_n'nnGTC
Cuts at: 3978 3978
Size: 7454
AleI CACnn'nnGTG
Cuts at: 2125 2416 4216 2125
Size: 291 1800 5363
Fragments arranged by size:
5363 1800 291
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 3469 3491 4204 3469
Size: 22 713 6719
Fragments arranged by size:
6719 713 22
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 4150 4182 4150
Size: 32 7422
AlwNI CAG_nnn'CTG
Cuts at: 164 272 1552 2657 2693 2826 4759 6326 164
Size: 108 1280 1105 36 133 1933 1567 1292
Fragments arranged by size:
1933 1567 1292 1280 1105 133 108 36
ApaI G_GGCC'C
Cuts at: 4119 4129 4140 4119
Size: 10 11 7433
Fragments arranged by size:
7433 11 10
ApaLI G'TGCA_C
Cuts at: 4249 6224 4249
Size: 1975 5479
ApoI r'AATT_y
Cuts at: 895 1041 1583 4079 4458 6807 7063 895
Size: 146 542 2496 379 2349 256 1286
Fragments arranged by size:
2496 2349 1286 542 379 256 146
AseI AT'TA_AT
Cuts at: 20 2849 20
Size: 2829 4625
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 7454
AvaI C'yCGr_G
Cuts at: 177 469 2275 2414 2453 3951 4286 4430 4474
Size: 292 1806 139 39 1498 335 144 44
Cuts at: 4474 177
Size: 3157
Fragments arranged by size:
3157 1806 1498 335 292 144 139 44
39
AvrII C'CTAG_G
Cuts at: 852 852
Size: 7454
BamHI G'GATC_C
Cuts at: 4479 4479
Size: 7454
BanI G'GyrC_C
Cuts at: 172 1507 2407 3493 4470 4884 5132 5167 172
Size: 1335 900 1086 977 414 248 35 2459
Fragments arranged by size:
2459 1335 1086 977 900 414 248 35
BanII G_rGCy'C
Cuts at: 137 2212 2282 3011 4119 4129 4140 4468 5498
Size: 2075 70 729 1108 10 11 328 1030
Cuts at: 5498 137
Size: 2093
Fragments arranged by size:
2093 2075 1108 1030 729 328 70 11
10
BbeI G_GCGC'C
Cuts at: 3497 4888 5136 3497
Size: 1391 248 5815
Fragments arranged by size:
5815 1391 248
BbsI GAAGACnn'nnnn_
Cuts at: 3234 3888 3234
Size: 654 6800
BbvCI CC'TCA_GC
Cuts at: 962 2197 3808 962
Size: 1235 1611 4608
Fragments arranged by size:
4608 1611 1235
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 3210 3244 3210
Size: 34 7420
BciVI GTATCCnnnnn_n'
Cuts at: 5352 6113 7454 5352
Size: 761 1341 5352
Fragments arranged by size:
5352 1341 761
BclI T'GATC_A
Cuts at: 4974 4974
Size: 7454
BfrBI ATG'CAT
Cuts at: 2680 2680
Size: 7454
BglI GCCn_nnn'nGGC
Cuts at: 339 1750 2405 2920 339
Size: 1411 655 515 4873
Fragments arranged by size:
4873 1411 655 515
BglII A'GATC_T
Cuts at: 2159 4969 7006 2159
Size: 2810 2037 2607
Fragments arranged by size:
2810 2607 2037
BlpI GC'TnA_GC
Cuts at: 2144 4566 2144
Size: 2422 5032
Bme1580I G_kGCm'C
Cuts at: 177 3353 4119 4129 4140 4193 4253 5079 5172
Size: 3176 766 10 11 53 60 826 93
Cuts at: 5172 6228 177
Size: 1056 1403
Fragments arranged by size:
3176 1403 1056 826 766 93 60 53
11 10
BmgBI CAC'GTC
Cuts at: 1760 4359 4410 1760
Size: 2599 51 4804
Fragments arranged by size:
4804 2599 51
BmrI ACTGGGnnnn_n'
Cuts at: 385 1570 2241 2703 3018 4666 4747 5082 7226
Size: 1185 671 462 315 1648 81 335 2144
Cuts at: 7226 385
Size: 613
Fragments arranged by size:
2144 1648 1185 671 613 462 335 315
81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 1269 2169 4312 5821 1269
Size: 900 2143 1509 2902
Fragments arranged by size:
2902 2143 1509 900
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 212 313 640 1315 1816 2244 3484 3647 212
Size: 101 327 675 501 428 1240 163 4019
Fragments arranged by size:
4019 1240 675 501 428 327 163 101
Bpu10I CC'TnA_GC
Cuts at: 962 2197 3701 3808 962
Size: 1235 1504 107 4608
Fragments arranged by size:
4608 1504 1235 107
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 4630 5509 6001 6299 4630
Size: 879 492 298 5785
Fragments arranged by size:
5785 879 492 298
BsaI GGTCTCn'nnnn_
Cuts at: 466 7158 466
Size: 6692 762
BsaAI yAC'GTr
Cuts at: 2127 4347 4807 2127
Size: 2220 460 4774
Fragments arranged by size:
4774 2220 460
BsaBI GATnn'nnATC
Cuts at: 6 1907 4968 7005 6
Size: 1901 3061 2037 455
Fragments arranged by size:
3061 2037 1901 455
BsaHI Gr'CG_yC
Cuts at: 278 1415 1526 3494 4885 5133 5835 7331 278
Size: 1137 111 1968 1391 248 702 1496 401
Fragments arranged by size:
1968 1496 1391 1137 702 401 248 111
BsaWI w'CCGG_w
Cuts at: 4361 4643 5164 5842 6116 6263 6674 4361
Size: 282 521 678 274 147 411 5141
Fragments arranged by size:
5141 678 521 411 282 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 475 497 505 527 1733 1763 2227 2257 2571
Size: 22 8 22 1206 30 464 30 314
Cuts at: 2571 2601 3457 3487 475
Size: 30 856 30 4442
Fragments arranged by size:
4442 1206 856 464 314 30 30 30
30 22 22 8
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 415 499 1138 3829 4386 4435 415
Size: 84 639 2691 557 49 3434
Fragments arranged by size:
3434 2691 639 557 84 49
BseYI C'CCAG_C
Cuts at: 265 938 1484 2388 2854 3510 3712 3836 4121
Size: 673 546 904 466 656 202 124 285
Cuts at: 4121 4161 4879 5637 6214 6710 265
Size: 40 718 758 577 496 1009
Fragments arranged by size:
1009 904 758 718 673 656 577 546
496 466 285 202 124 40
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 241 2188 2468 2626 3204 241
Size: 1947 280 158 578 4491
Fragments arranged by size:
4491 1947 578 280 158
BsiEI CG_ry'CG
Cuts at: 104 187 1007 3266 3302 4996 5042 6250 104
Size: 83 820 2259 36 1694 46 1208 1308
Fragments arranged by size:
2259 1694 1308 1208 820 83 46 36
BsiHKAI G_wGCw'C
Cuts at: 1232 1573 2221 3470 4253 4468 5246 5436 6228
Size: 341 648 1249 783 215 778 190 792
Cuts at: 6228 1232
Size: 2458
Fragments arranged by size:
2458 1249 792 783 778 648 341 215
190
BsmI GAATG_Cn'
Cuts at: 4095 4420 4095
Size: 325 7129
BsmBI CGTCTCn'nnnn_
Cuts at: 2417 4061 2417
Size: 1644 5810
Bsp1286I G_dGCh'C
Cuts at: 137 177 1232 1573 2212 2221 2282 3011 3353
Size: 40 1055 341 639 9 61 729 342
Cuts at: 3353 3470 4119 4129 4140 4193 4253 4468 5079
Size: 117 649 10 11 53 60 215 611
Cuts at: 5079 5172 5246 5436 5498 6228 137
Size: 93 74 190 62 730 1363
Fragments arranged by size:
1363 1055 730 729 649 639 611 342
341 215 190 117 93 74 62 61
60 53 40 11 10 9
BspEI T'CCGG_A
Cuts at: 6674 6674
Size: 7454
BspHI T'CATG_A
Cuts at: 578 6630 578
Size: 6052 1402
BspMI ACCTGCnnnn'nnnn_
Cuts at: 2246 4504 4718 5020 5401 2246
Size: 2258 214 302 381 4299
Fragments arranged by size:
4299 2258 381 302 214
BsrBI CCG'CTC
Cuts at: 50 1813 5746 5800 7284 7449 50
Size: 1763 3933 54 1484 165 55
Fragments arranged by size:
3933 1763 1484 165 55 54
BsrDI GCAATG_nn'
Cuts at: 1776 5366 1776
Size: 3590 3864
BsrFI r'CCGG_y
Cuts at: 186 260 2776 5452 5633 5842 186
Size: 74 2516 2676 181 209 1798
Fragments arranged by size:
2676 2516 1798 209 181 74
BsrGI T'GTAC_A
Cuts at: 683 683
Size: 7454
BssSI C'ACGA_G
Cuts at: 3229 5725 6083 3229
Size: 2496 358 4600
Fragments arranged by size:
4600 2496 358
BstAPI GCAn_nnn'nTGC
Cuts at: 4831 4831
Size: 7454
BstBI TT'CG_AA
Cuts at: 4381 5815 4381
Size: 1434 6020
BstXI CCAn_nnnn'nTGG
Cuts at: 547 1386 2361 3510 4176 547
Size: 839 975 1149 666 3825
Fragments arranged by size:
3825 1149 975 839 666
BstYI r'GATC_y
Cuts at: 1 2159 3532 4479 4946 4969 5304 5550 6562
Size: 2158 1373 947 467 23 335 246 1012
Cuts at: 6562 6648 6660 7006 1
Size: 86 12 346 449
Fragments arranged by size:
2158 1373 1012 947 467 449 346 335
246 86 23 12
BstZ17I GTA'TAC
Cuts at: 678 678
Size: 7454
Bsu36I CC'TnA_GG
Cuts at: 1988 3653 1988
Size: 1665 5789
BtgI C'CryG_G
Cuts at: 107 144 1511 1865 3353 4175 4323 107
Size: 37 1367 354 1488 822 148 3238
Fragments arranged by size:
3238 1488 1367 822 354 148 37
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 5404 5585 5748 5404
Size: 181 163 7110
Fragments arranged by size:
7110 181 163
BtsI GCAGTG_nn'
Cuts at: 700 2268 4169 4697 700
Size: 1568 1901 528 3457
Fragments arranged by size:
3457 1901 1568 528
DraI TTT'AAA
Cuts at: 372 4453 372
Size: 4081 3373
DraIII CAC_nnn'GTG
Cuts at: 3867 3867
Size: 7454
DrdI GACnn_nn'nnGTC
Cuts at: 515 1420 2746 3550 3798 5160 6018 515
Size: 905 1326 804 248 1362 858 1951
Fragments arranged by size:
1951 1362 1326 905 858 804 248
EaeI y'GGCC_r
Cuts at: 184 321 1004 1433 1790 2932 3263 3425 3741
Size: 137 683 429 357 1142 331 162 316
Cuts at: 3741 4042 5039 5213 5604 5631 6976 184
Size: 301 997 174 391 27 1345 662
Fragments arranged by size:
1345 1142 997 683 662 429 391 357
331 316 301 174 162 137 27
EagI C'GGCC_G
Cuts at: 184 1004 3263 5039 184
Size: 820 2259 1776 2599
Fragments arranged by size:
2599 2259 1776 820
EarI CTCTTCn'nnn_
Cuts at: 548 899 3160 5477 5687 548
Size: 351 2261 2317 210 2315
Fragments arranged by size:
2317 2315 2261 351 210
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 3169 4293 5662 5972 6118 7264 3169
Size: 1124 1369 310 146 1146 3359
Fragments arranged by size:
3359 1369 1146 1124 310 146
EcoICRI GAG'CTC
Cuts at: 4466 4466
Size: 7454
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 212 313 640 1315 1656 1750 1816 2244 2634
Size: 101 327 675 341 94 66 428 390
Cuts at: 2634 2647 2656 3484 3647 4109 5279 5711 6458
Size: 13 9 828 163 462 1170 432 747
Cuts at: 6458 6726 212
Size: 268 940
Fragments arranged by size:
1170 940 828 747 675 462 432 428
390 341 327 268 163 101 94 66
13 9
EcoNI CCTnn'n_nnAGG
Cuts at: 1305 3811 4333 1305
Size: 2506 522 4426
Fragments arranged by size:
4426 2506 522
EcoO109I rG'GnC_Cy
Cuts at: 437 2235 3853 3980 4020 4115 4125 4126 4136
Size: 1798 1618 127 40 95 10 1 10
Cuts at: 4136 4338 4427 4593 437
Size: 202 89 166 3298
Fragments arranged by size:
3298 1798 1618 202 166 127 95 89
40 10 10 1
EcoRI G'AATT_C
Cuts at: 895 4458 895
Size: 3563 3891
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 1718 5473 1718
Size: 3755 3699
FspI TGC'GCA
Cuts at: 5235 5235
Size: 7454
HaeII r_GCGC'y
Cuts at: 334 631 1178 2861 3292 3497 4888 5136 6158
Size: 297 547 1683 431 205 1391 248 1022
Cuts at: 6158 7137 334
Size: 979 651
Fragments arranged by size:
1683 1391 1022 979 651 547 431 297
248 205
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 719 751 2227 2259 3964 3996 5296 5326 5328
Size: 32 1476 32 1705 32 1300 30 2
Cuts at: 5328 5358 719
Size: 30 2815
Fragments arranged by size:
2815 1705 1476 1300 32 32 32 30
30 2
HincII GTy'rAC
Cuts at: 2813 4493 2813
Size: 1680 5774
KasI G'GCGC_C
Cuts at: 3493 4884 5132 3493
Size: 1391 248 5815
Fragments arranged by size:
5815 1391 248
KpnI G_GTAC'C
Cuts at: 4474 4474
Size: 7454
MfeI C'AATT_G
Cuts at: 3077 3077
Size: 7454
MluI A'CGCG_T
Cuts at: 6887 6887
Size: 7454
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 483 2279 2588 3794 4469 6125 6309 483
Size: 1796 309 1206 675 1656 184 1628
Fragments arranged by size:
1796 1656 1628 1206 675 309 184
MscI TGG'CCA
Cuts at: 1435 4044 5215 1435
Size: 2609 1171 3674
Fragments arranged by size:
3674 2609 1171
MslI CAynn'nnrTG
Cuts at: 1334 1429 2125 2416 3205 3277 3385 4174 4216
Size: 95 696 291 789 72 108 789 42
Cuts at: 4216 5443 5570 1334
Size: 1227 127 3218
Fragments arranged by size:
3218 1227 789 789 696 291 127 108
95 72 42
MspA1I CmG'CkG
Cuts at: 146 161 336 938 1439 1840 2143 2201 3755
Size: 15 175 602 501 401 303 58 1554
Cuts at: 3755 4121 4565 4879 5239 6252 6497 146
Size: 366 444 314 360 1013 245 1103
Fragments arranged by size:
1554 1103 1013 602 501 444 401 366
360 314 303 245 175 58 15
NaeI GCC'GGC
Cuts at: 188 5635 188
Size: 5447 2007
NarI GG'CG_CC
Cuts at: 3494 4885 5133 3494
Size: 1391 248 5815
Fragments arranged by size:
5815 1391 248
NcoI C'CATG_G
Cuts at: 107 1865 4175 107
Size: 1758 2310 3386
Fragments arranged by size:
3386 2310 1758
NdeI CA'TA_TG
Cuts at: 1314 1314
Size: 7454
NgoMIV G'CCGG_C
Cuts at: 186 5633 186
Size: 5447 2007
NsiI A_TGCA'T
Cuts at: 2682 2682
Size: 7454
NspI r_CATG'y
Cuts at: 684 2517 2976 3474 4507 5007 5914 684
Size: 1833 459 498 1033 500 907 2224
Fragments arranged by size:
2224 1833 1033 907 500 498 459
PasI CC'CwG_GG
Cuts at: 1503 4267 1503
Size: 2764 4690
PciI A'CATG_T
Cuts at: 680 2513 3470 5910 680
Size: 1833 957 2440 2224
Fragments arranged by size:
2440 2224 1833 957
PflMI CCAn_nnn'nTGG
Cuts at: 2636 2636
Size: 7454
PfoI T'CCnGG_A
Cuts at: 899 1292 3485 3990 899
Size: 393 2193 505 4363
Fragments arranged by size:
4363 2193 505 393
PmeI GTTT'AAAC
Cuts at: 4453 4453
Size: 7454
PmlI CAC'GTG
Cuts at: 2127 4347 2127
Size: 2220 5234
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 6593 6625 6593
Size: 32 7422
PpuMI rG'GwC_Cy
Cuts at: 2235 3980 4020 4338 2235
Size: 1745 40 318 5351
Fragments arranged by size:
5351 1745 318 40
PspOMI G'GGCC_C
Cuts at: 4115 4125 4136 4115
Size: 10 11 7433
Fragments arranged by size:
7433 11 10
PspXI vC'TCGA_Gb
Cuts at: 2275 2275
Size: 7454
PsrI GAACnnnnnnTACnnnnnnn_nnnnn'
Cuts at: 3010 3042 3010
Size: 32 7422
PstI C_TGCA'G
Cuts at: 1190 1557 3103 4406 4501 5186 1190
Size: 367 1546 1303 95 685 3458
Fragments arranged by size:
3458 1546 1303 685 367 95
PvuI CG_AT'CG
Cuts at: 104 104
Size: 7454
PvuII CAG'CTG
Cuts at: 938 2201 4879 5239 938
Size: 1263 2678 360 3153
Fragments arranged by size:
3153 2678 1263 360
RsrII CG'GwC_CG
Cuts at: 243 5649 243
Size: 5406 2048
SacI G_AGCT'C
Cuts at: 4468 4468
Size: 7454
SacII CC_GC'GG
Cuts at: 147 147
Size: 7454
SalI G'TCGA_C
Cuts at: 4491 4491
Size: 7454
SanDI GG'GwC_CC
Cuts at: 3980 4020 3980
Size: 40 7414
SapI GCTCTTCn'nnn_
Cuts at: 5477 5687 5477
Size: 210 7244
SbfI CC_TGCA'GG
Cuts at: 4501 4501
Size: 7454
ScaI AGT'ACT
Cuts at: 6971 6971
Size: 7454
SfcI C'TryA_G
Cuts at: 33 1186 1553 2022 2040 3099 4053 4402 4497
Size: 1153 367 469 18 1059 954 349 95
Cuts at: 4497 5182 6175 6366 33
Size: 685 993 191 1121
Fragments arranged by size:
1153 1121 1059 993 954 685 469 367
349 191 95 18
SfoI GGC'GCC
Cuts at: 3495 4886 5134 3495
Size: 1391 248 5815
Fragments arranged by size:
5815 1391 248
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 7454
SmaI CCC'GGG
Cuts at: 179 4432 4476 179
Size: 4253 44 3157
Fragments arranged by size:
4253 3157 44
SmlI C'TyrA_G
Cuts at: 469 2275 4609 5524 6016 6278 469
Size: 1806 2334 915 492 262 1645
Fragments arranged by size:
2334 1806 1645 915 492 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 7454
SphI G_CATG'C
Cuts at: 4507 5007 4507
Size: 500 6954
StuI AGG'CCT
Cuts at: 1300 1993 3806 1300
Size: 693 1813 4948
Fragments arranged by size:
4948 1813 693
StyI C'CwwG_G
Cuts at: 107 305 852 1865 2354 4175 4588 4942 107
Size: 198 547 1013 489 1821 413 354 2619
Fragments arranged by size:
2619 1821 1013 547 489 413 354 198
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 483 1126 2542 3953 5037 5170 5629 5838 483
Size: 643 1416 1411 1084 133 459 209 2099
Fragments arranged by size:
2099 1416 1411 1084 643 459 209 133
TatI w'GTAC_w
Cuts at: 683 2129 3167 6969 683
Size: 1446 1038 3802 1168
Fragments arranged by size:
3802 1446 1168 1038
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 475 507 697 729 1733 1765 6971 7003 475
Size: 32 190 32 1004 32 5206 32 926
Fragments arranged by size:
5206 1004 926 190 32 32 32 32
Tth111I GACn'n_nGTC
Cuts at: 2899 3798 5251 2899
Size: 899 1453 5102
Fragments arranged by size:
5102 1453 899
XbaI T'CTAG_A
Cuts at: 4485 4485
Size: 7454
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 1383 4949 1383
Size: 3566 3888
XhoI C'TCGA_G
Cuts at: 469 2275 469
Size: 1806 5648
XmaI C'CCGG_G
Cuts at: 177 4430 4474 177
Size: 4253 44 3157
Fragments arranged by size:
4253 3157 44
XmnI GAAnn'nnTTC
Cuts at: 1405 1405
Size: 7454
ZraI GAC'GTC
Cuts at: 1416 1416
Size: 7454
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AfeI AflIII AgeI
AhdI AleI AlfI AloI AlwNI ApaI ApaLI ApoI
AseI AsiSI AvaI AvrII BamHI BanI BanII BbeI
BbsI BbvCI BcgI BciVI BclI BfrBI BglI BglII
BlpI Bme1580I BmgBI BmrI BplI BpmI Bpu10I BpuEI
BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI
BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI
BspMI BsrBI BsrDI BsrFI BsrGI BssSI BstAPI BstBI
BstXI BstYI BstZ17I Bsu36I BtgI BtgZI BtsI DraI
DraIII DrdI EaeI EagI EarI EciI EcoICRI Eco57MI
EcoNI EcoO109I EcoRI FalI FspI HaeII Hin4I HincII
KasI KpnI MfeI MluI MmeI MscI MslI MspA1I
NaeI NarI NcoI NdeI NgoMIV NsiI NspI PasI
PciI PflMI PfoI PmeI PmlI PpiI PpuMI PspOMI
PspXI PsrI PstI PvuI PvuII RsrII SacI SacII
SalI SanDI SapI SbfI ScaI SfcI SfoI SgfI
SmaI SmlI SpeI SphI StuI StyI TaqII TatI
TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AarI AflII AscI BaeI BmtI BsiWI BssHII BstEII
ClaI CspCI EcoRV FseI FspAI HindIII HpaI NheI
NotI NruI PacI PshAI PsiI SexAI SfiI SgrAI
SnaBI SrfI SspI SwaI