Restriction map for
pF1KE0076
(Circular) (Six-base) MAPSORT of: pF1KE0076.seq Check: 8236 from: 1 to: 7922
pF1KE0076 7922 bp
With 182 enzymes: SgfI *
April 24, 2009 13:10 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 2358 2389 4318 2358
Size: 31 1929 5962
Fragments arranged by size:
5962 1929 31
AatII G_ACGT'C
Cuts at: 1136 1952 1136
Size: 816 7106
AccI GT'mk_AC
Cuts at: 2300 4960 2300
Size: 2660 5262
Acc65I G'GTAC_C
Cuts at: 3315 4508 4802 4938 3315
Size: 1193 294 136 6299
Fragments arranged by size:
6299 1193 294 136
AclI AA'CG_TT
Cuts at: 7762 7762
Size: 7922
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1690 2163 2897 3204 5747 6179 6926 7194 1690
Size: 473 734 307 2543 432 747 268 2418
Fragments arranged by size:
2543 2418 747 734 473 432 307 268
AflIII A'CryG_T
Cuts at: 5274 6378 7355 5274
Size: 1104 977 5841
Fragments arranged by size:
5841 1104 977
AgeI A'CCGG_T
Cuts at: 405 6310 405
Size: 5905 2017
AleI CACnn'nnGTG
Cuts at: 530 530
Size: 7922
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1031 1031
Size: 7922
AlwNI CAG_nnn'CTG
Cuts at: 129 519 793 933 1056 1167 1825 1887 2236
Size: 390 274 140 123 111 658 62 349
Cuts at: 2236 2404 3337 3355 4711 5227 6794 129
Size: 168 933 18 1356 516 1567 1257
Fragments arranged by size:
1567 1356 1257 933 658 516 390 349
274 168 140 123 111 62 18
ApaI G_GGCC'C
Cuts at: 2251 2439 3190 3240 3911 4849 4871 2251
Size: 188 751 50 671 938 22 5302
Fragments arranged by size:
5302 938 751 671 188 50 22
ApaLI G'TGCA_C
Cuts at: 2044 2567 6692 2044
Size: 523 4125 3274
Fragments arranged by size:
4125 3274 523
ApoI r'AATT_y
Cuts at: 4926 7275 7531 4926
Size: 2349 256 5317
Fragments arranged by size:
5317 2349 256
AseI AT'TA_AT
Cuts at: 20 20
Size: 7922
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 7922
AvaI C'yCGr_G
Cuts at: 898 913 979 1228 1333 2250 2501 3394 4225
Size: 15 66 249 105 917 251 893 831
Cuts at: 4225 4888 4942 898
Size: 663 54 3878
Fragments arranged by size:
3878 917 893 831 663 251 249 105
66 54 15
AvrII C'CTAG_G
Cuts at: 718 718
Size: 7922
BamHI G'GATC_C
Cuts at: 4947 4947
Size: 7922
BanI G'GyrC_C
Cuts at: 323 2270 2370 2450 2713 2949 3315 3325 4177
Size: 1947 100 80 263 236 366 10 852
Cuts at: 4177 4508 4802 4938 5352 5600 5635 323
Size: 331 294 136 414 248 35 2610
Fragments arranged by size:
2610 1947 852 414 366 331 294 263
248 236 136 100 80 35 10
BanII G_rGCy'C
Cuts at: 557 1157 1193 1658 2251 2439 2591 3190 3240
Size: 600 36 465 593 188 152 599 50
Cuts at: 3240 3524 3911 4379 4849 4871 4936 5966 557
Size: 284 387 468 470 22 65 1030 2513
Fragments arranged by size:
2513 1030 600 599 593 470 468 465
387 284 188 152 65 50 36 22
BbeI G_GCGC'C
Cuts at: 327 5356 5604 327
Size: 5029 248 2645
Fragments arranged by size:
5029 2645 248
BbsI GAAGACnn'nnnn_
Cuts at: 1586 1698 1586
Size: 112 7810
BbvCI CC'TCA_GC
Cuts at: 926 2961 926
Size: 2035 5887
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 90 124 90
Size: 34 7888
BciVI GTATCCnnnnn_n'
Cuts at: 5820 6581 7922 5820
Size: 761 1341 5820
Fragments arranged by size:
5820 1341 761
BclI T'GATC_A
Cuts at: 5442 5442
Size: 7922
BglI GCCn_nnn'nGGC
Cuts at: 1170 1368 1828 1843 1963 3438 3472 1170
Size: 198 460 15 120 1475 34 5620
Fragments arranged by size:
5620 1475 460 198 120 34 15
BglII A'GATC_T
Cuts at: 5437 7474 5437
Size: 2037 5885
BlpI GC'TnA_GC
Cuts at: 1652 2590 3948 4521 5034 1652
Size: 938 1358 573 513 4540
Fragments arranged by size:
4540 1358 938 573 513
Bme1580I G_kGCm'C
Cuts at: 1175 2048 2251 2273 2375 2439 2453 2571 2716
Size: 873 203 22 102 64 14 118 145
Cuts at: 2716 2742 2954 3190 3240 3328 3911 4498 4614
Size: 26 212 236 50 88 583 587 116
Cuts at: 4614 4849 4871 5547 5640 6696 1175
Size: 235 22 676 93 1056 2401
Fragments arranged by size:
2401 1056 873 676 587 583 236 235
212 203 145 118 116 102 93 88
64 50 26 22 22 14
BmgBI CAC'GTC
Cuts at: 3513 3513
Size: 7922
BmrI ACTGGGnnnn_n'
Cuts at: 614 1477 2021 2321 2369 2528 2791 3009 3471
Size: 863 544 300 48 159 263 218 462
Cuts at: 3471 3491 3615 3914 4333 5134 5215 5550 7694
Size: 20 124 299 419 801 81 335 2144
Cuts at: 7694 614
Size: 842
Fragments arranged by size:
2144 863 842 801 544 462 419 335
300 299 263 218 159 124 81 48
20
BmtI G_CTAG'C
Cuts at: 4628 4628
Size: 7922
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 870 3612 3624 6289 870
Size: 2742 12 2665 2503
Fragments arranged by size:
2742 2665 2503 12
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1012 1033 1171 2545 2888 3730 4378 4499 1012
Size: 21 138 1374 343 842 648 121 4435
Fragments arranged by size:
4435 1374 842 648 343 138 121 21
Bpu10I CC'TnA_GC
Cuts at: 707 713 794 926 1612 2961 4438 707
Size: 6 81 132 686 1349 1477 4191
Fragments arranged by size:
4191 1477 1349 686 132 81 6
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1620 3660 4366 5098 5977 6469 6767 1620
Size: 2040 706 732 879 492 298 2775
Fragments arranged by size:
2775 2040 879 732 706 492 298
BsaI GGTCTCn'nnnn_
Cuts at: 1449 1494 2926 3583 4115 7626 1449
Size: 45 1432 657 532 3511 1745
Fragments arranged by size:
3511 1745 1432 657 532 45
BsaAI yAC'GTr
Cuts at: 5275 5275
Size: 7922
BsaBI GATnn'nnATC
Cuts at: 6 484 5436 7473 6
Size: 478 4952 2037 455
Fragments arranged by size:
4952 2037 478 455
BsaHI Gr'CG_yC
Cuts at: 324 830 1133 1325 1919 1949 4280 5353 5601
Size: 506 303 192 594 30 2331 1073 248
Cuts at: 5601 6303 7799 324
Size: 702 1496 447
Fragments arranged by size:
2331 1496 1073 702 594 506 447 303
248 192 30
BsaWI w'CCGG_w
Cuts at: 405 5111 5632 6310 6584 6731 7142 405
Size: 4706 521 678 274 147 411 1185
Fragments arranged by size:
4706 1185 678 521 411 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 262 292 4585 4615 262
Size: 30 4293 30 3569
Fragments arranged by size:
4293 3569 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 603 664 1400 2643 3354 3357 3360 3363 3366
Size: 61 736 1243 711 3 3 3 3
Cuts at: 3366 603
Size: 5159
Fragments arranged by size:
5159 1243 736 711 61 3 3 3
3
BseYI C'CCAG_C
Cuts at: 313 437 685 731 875 941 1157 1560 1632
Size: 124 248 46 144 66 216 403 72
Cuts at: 1632 1773 1898 2236 2432 2750 2821 3142 4210
Size: 141 125 338 196 318 71 321 1068
Cuts at: 4210 4458 4483 5347 6105 6682 7178 313
Size: 248 25 864 758 577 496 1057
Fragments arranged by size:
1068 1057 864 758 577 496 403 338
321 318 248 248 216 196 144 141
125 124 72 71 66 46 25
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 174 493 560 2113 174
Size: 319 67 1553 5983
Fragments arranged by size:
5983 1553 319 67
BsiEI CG_ry'CG
Cuts at: 104 410 1752 1814 5464 5510 6718 104
Size: 306 1342 62 3650 46 1208 1308
Fragments arranged by size:
3650 1342 1308 1208 306 62 46
BsiHKAI G_wGCw'C
Cuts at: 1213 2005 2048 2571 2591 3904 4224 4936 5714
Size: 792 43 523 20 1313 320 712 778
Cuts at: 5714 5904 6696 1213
Size: 190 792 2439
Fragments arranged by size:
2439 1313 792 792 778 712 523 320
190 43 20
BsmBI CGTCTCn'nnnn_
Cuts at: 407 2325 407
Size: 1918 6004
Bsp1286I G_dGCh'C
Cuts at: 557 1157 1175 1193 1213 1658 2005 2048 2251
Size: 600 18 18 20 445 347 43 203
Cuts at: 2251 2273 2375 2439 2453 2571 2591 2716 2742
Size: 22 102 64 14 118 20 125 26
Cuts at: 2742 2954 3190 3240 3328 3524 3904 3911 4224
Size: 212 236 50 88 196 380 7 313
Cuts at: 4224 4379 4498 4614 4849 4871 4936 5547 5640
Size: 155 119 116 235 22 65 611 93
Cuts at: 5640 5714 5904 5966 6696 557
Size: 74 190 62 730 1783
Fragments arranged by size:
1783 730 611 600 445 380 347 313
236 235 212 203 196 190 155 125
119 118 116 102 93 88 74 65
64 62 50 43 26 22 22 20
20 18 18 14 7
BspEI T'CCGG_A
Cuts at: 7142 7142
Size: 7922
BspHI T'CATG_A
Cuts at: 488 7098 488
Size: 6610 1312
BspMI ACCTGCnnnn'nnnn_
Cuts at: 467 527 1775 2358 2389 3017 4318 4460 4972
Size: 60 1248 583 31 628 1301 142 512
Cuts at: 4972 5186 5488 5869 467
Size: 214 302 381 2520
Fragments arranged by size:
2520 1301 1248 628 583 512 381 302
214 142 60 31
BsrBI CCG'CTC
Cuts at: 50 654 681 978 2975 3963 4856 6214 6268
Size: 604 27 297 1997 988 893 1358 54
Cuts at: 6268 7752 7917 50
Size: 1484 165 55
Fragments arranged by size:
1997 1484 1358 988 893 604 297 165
55 54 27
BsrDI GCAATG_nn'
Cuts at: 5834 5834
Size: 7922
BsrFI r'CCGG_y
Cuts at: 405 1292 2617 3160 3633 4282 5920 6101 6310
Size: 887 1325 543 473 649 1638 181 209
Cuts at: 6310 405
Size: 2017
Fragments arranged by size:
2017 1638 1325 887 649 543 473 209
181
BsrGI T'GTAC_A
Cuts at: 534 534
Size: 7922
BssHII G'CGCG_C
Cuts at: 2857 2857
Size: 7922
BssSI C'ACGA_G
Cuts at: 6193 6551 6193
Size: 358 7564
BstAPI GCAn_nnn'nTGC
Cuts at: 2091 3098 3355 3745 4711 5299 2091
Size: 1007 257 390 966 588 4714
Fragments arranged by size:
4714 1007 966 588 390 257
BstBI TT'CG_AA
Cuts at: 6283 6283
Size: 7922
BstEII G'GTnAC_C
Cuts at: 1240 1240
Size: 7922
BstXI CCAn_nnnn'nTGG
Cuts at: 1371 1993 2523 2799 4362 1371
Size: 622 530 276 1563 4931
Fragments arranged by size:
4931 1563 622 530 276
BstYI r'GATC_y
Cuts at: 1 1280 1547 4947 5414 5437 5772 6018 7030
Size: 1279 267 3400 467 23 335 246 1012
Cuts at: 7030 7116 7128 7474 1
Size: 86 12 346 449
Fragments arranged by size:
3400 1279 1012 467 449 346 335 267
246 86 23 12
Bsu36I CC'TnA_GG
Cuts at: 647 1235 2538 2574 3879 647
Size: 588 1303 36 1305 4690
Fragments arranged by size:
4690 1305 1303 588 36
BtgI C'CryG_G
Cuts at: 364 761 773 1364 1841 2225 2445 2544 2966
Size: 397 12 591 477 384 220 99 422
Cuts at: 2966 3804 4268 4692 4871 364
Size: 838 464 424 179 3415
Fragments arranged by size:
3415 838 591 477 464 424 422 397
384 220 179 99 12
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 893 5872 6053 6216 893
Size: 4979 181 163 2599
Fragments arranged by size:
4979 2599 181 163
BtsI GCAGTG_nn'
Cuts at: 1173 2810 3123 4081 4197 5165 1173
Size: 1637 313 958 116 968 3930
Fragments arranged by size:
3930 1637 968 958 313 116
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 4257 4292 4257
Size: 35 7887
DraI TTT'AAA
Cuts at: 4921 4921
Size: 7922
DraIII CAC_nnn'GTG
Cuts at: 1717 2029 4693 1717
Size: 312 2664 4946
Fragments arranged by size:
4946 2664 312
DrdI GACnn_nn'nnGTC
Cuts at: 3511 3582 5628 6486 3511
Size: 71 2046 858 4947
Fragments arranged by size:
4947 2046 858 71
EaeI y'GGCC_r
Cuts at: 626 738 770 1184 1385 1541 1749 1811 1835
Size: 112 32 414 201 156 208 62 24
Cuts at: 1835 1844 1955 2136 2156 2222 2469 2762 3977
Size: 9 111 181 20 66 247 293 1215
Cuts at: 3977 4185 4345 5507 5681 6072 6099 7444 626
Size: 208 160 1162 174 391 27 1345 1104
Fragments arranged by size:
1345 1215 1162 1104 414 391 293 247
208 208 201 181 174 160 156 112
111 66 62 32 27 24 20 9
EagI C'GGCC_G
Cuts at: 1749 1811 5507 1749
Size: 62 3696 4164
Fragments arranged by size:
4164 3696 62
EarI CTCTTCn'nnn_
Cuts at: 2617 4331 5945 6155 2617
Size: 1714 1614 210 4384
Fragments arranged by size:
4384 1714 1614 210
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 1311 1584 1704 1890 2474 2842 3828 3852 4891
Size: 273 120 186 584 368 986 24 1039
Cuts at: 4891 6130 6440 6586 7732 1311
Size: 1239 310 146 1146 1501
Fragments arranged by size:
1501 1239 1146 1039 986 584 368 310
273 186 146 120 24
EcoICRI GAG'CTC
Cuts at: 2589 4934 2589
Size: 2345 5577
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1012 1033 1171 1690 2163 2545 2888 2897 3204
Size: 21 138 519 473 382 343 9 307
Cuts at: 3204 3730 4378 4499 5747 6179 6926 7194 1012
Size: 526 648 121 1248 432 747 268 1740
Fragments arranged by size:
1740 1248 747 648 526 519 473 432
382 343 307 268 138 121 21 9
EcoNI CCTnn'n_nnAGG
Cuts at: 340 511 740 3832 4291 340
Size: 171 229 3092 459 3971
Fragments arranged by size:
3971 3092 459 229 171
EcoO109I rG'GnC_Cy
Cuts at: 126 309 495 516 894 1053 1777 2052 2247
Size: 183 186 21 378 159 724 275 195
Cuts at: 2247 2436 2757 3186 3187 3236 3237 3908 3941
Size: 189 321 429 1 49 1 671 33
Cuts at: 3941 4100 4141 4449 4845 4846 4867 5061 126
Size: 159 41 308 396 1 21 194 2987
Fragments arranged by size:
2987 724 671 429 396 378 321 308
275 195 194 189 186 183 159 159
49 41 33 21 21 1 1 1
EcoRI G'AATT_C
Cuts at: 4926 4926
Size: 7922
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 1272 1983 3231 4875 5941 1272
Size: 711 1248 1644 1066 3253
Fragments arranged by size:
3253 1644 1248 1066 711
FspI TGC'GCA
Cuts at: 5703 5703
Size: 7922
HaeII r_GCGC'y
Cuts at: 327 2604 3152 3161 5356 5604 6626 7605 327
Size: 2277 548 9 2195 248 1022 979 644
Fragments arranged by size:
2277 2195 1022 979 644 548 248 9
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 892 924 1195 1227 1272 1304 2023 2055 2638
Size: 32 271 32 45 32 719 32 583
Cuts at: 2638 2670 3451 3483 3676 3708 3727 3759 5764
Size: 32 781 32 193 32 19 32 2005
Cuts at: 5764 5794 5796 5826 892
Size: 30 2 30 2988
Fragments arranged by size:
2988 2005 781 719 583 271 193 45
32 32 32 32 32 32 32 32
30 30 19 2
HincII GTy'rAC
Cuts at: 4014 4961 4014
Size: 947 6975
KasI G'GCGC_C
Cuts at: 323 5352 5600 323
Size: 5029 248 2645
Fragments arranged by size:
5029 2645 248
KpnI G_GTAC'C
Cuts at: 3319 4512 4806 4942 3319
Size: 1193 294 136 6299
Fragments arranged by size:
6299 1193 294 136
MluI A'CGCG_T
Cuts at: 7355 7355
Size: 7922
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 390 582 656 3566 3569 3635 6593 6777 390
Size: 192 74 2910 3 66 2958 184 1535
Fragments arranged by size:
2958 2910 1535 192 184 74 66 3
MscI TGG'CCA
Cuts at: 628 740 1543 1846 1957 2764 4187 5683 628
Size: 112 803 303 111 807 1423 1496 2867
Fragments arranged by size:
2867 1496 1423 807 803 303 112 111
MslI CAynn'nnrTG
Cuts at: 112 530 1531 1846 5911 6038 112
Size: 418 1001 315 4065 127 1996
Fragments arranged by size:
4065 1996 1001 418 315 127
MspA1I CmG'CkG
Cuts at: 357 395 477 879 1089 1665 1801 1834 2015
Size: 38 82 402 210 576 136 33 181
Cuts at: 2015 2236 2754 2816 3874 3976 4254 4420 4458
Size: 221 518 62 1058 102 278 166 38
Cuts at: 4458 4487 4525 4711 5033 5347 5707 6720 6965
Size: 29 38 186 322 314 360 1013 245
Cuts at: 6965 357
Size: 1314
Fragments arranged by size:
1314 1058 1013 576 518 402 360 322
314 278 245 221 210 186 181 166
136 102 82 62 38 38 38 33
29
NaeI GCC'GGC
Cuts at: 1294 3162 4284 6103 1294
Size: 1868 1122 1819 3113
Fragments arranged by size:
3113 1868 1819 1122
NarI GG'CG_CC
Cuts at: 324 5353 5601 324
Size: 5029 248 2645
Fragments arranged by size:
5029 2645 248
NcoI C'CATG_G
Cuts at: 1841 2445 2966 4871 1841
Size: 604 521 1905 4892
Fragments arranged by size:
4892 1905 604 521
NgoMIV G'CCGG_C
Cuts at: 1292 3160 4282 6101 1292
Size: 1868 1122 1819 3113
Fragments arranged by size:
3113 1868 1819 1122
NheI G'CTAG_C
Cuts at: 4624 4624
Size: 7922
NotI GC'GGCC_GC
Cuts at: 1749 1749
Size: 7922
NspI r_CATG'y
Cuts at: 4077 4975 5475 6382 4077
Size: 898 500 907 5617
Fragments arranged by size:
5617 907 898 500
PasI CC'CwG_GG
Cuts at: 123 1167 123
Size: 1044 6878
PciI A'CATG_T
Cuts at: 6378 6378
Size: 7922
PflMI CCAn_nnn'nTGG
Cuts at: 129 1056 2389 4693 129
Size: 927 1333 2304 3358
Fragments arranged by size:
3358 2304 1333 927
PfoI T'CCnGG_A
Cuts at: 173 753 932 953 2338 2698 3533 4025 173
Size: 580 179 21 1385 360 835 492 4070
Fragments arranged by size:
4070 1385 835 580 492 360 179 21
PmeI GTTT'AAAC
Cuts at: 4921 4921
Size: 7922
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 7061 7093 7061
Size: 32 7890
PpuMI rG'GwC_Cy
Cuts at: 309 495 516 1777 4141 4449 309
Size: 186 21 1261 2364 308 3782
Fragments arranged by size:
3782 2364 1261 308 186 21
PspOMI G'GGCC_C
Cuts at: 2247 2435 3186 3236 3907 4845 4867 2247
Size: 188 751 50 671 938 22 5302
Fragments arranged by size:
5302 938 751 671 188 50 22
PspXI vC'TCGA_Gb
Cuts at: 898 898
Size: 7922
PstI C_TGCA'G
Cuts at: 524 3103 3351 4292 4425 4707 4787 4969 5654
Size: 2579 248 941 133 282 80 182 685
Cuts at: 5654 524
Size: 2792
Fragments arranged by size:
2792 2579 941 685 282 248 182 133
80
PvuI CG_AT'CG
Cuts at: 104 104
Size: 7922
PvuII CAG'CTG
Cuts at: 477 879 1089 1801 4420 4487 4525 5347 5707
Size: 402 210 712 2619 67 38 822 360
Cuts at: 5707 477
Size: 2692
Fragments arranged by size:
2692 2619 822 712 402 360 210 67
38
RsrII CG'GwC_CG
Cuts at: 6117 6117
Size: 7922
SacI G_AGCT'C
Cuts at: 2591 4936 2591
Size: 2345 5577
SalI G'TCGA_C
Cuts at: 4959 4959
Size: 7922
SanDI GG'GwC_CC
Cuts at: 309 1777 309
Size: 1468 6454
SapI GCTCTTCn'nnn_
Cuts at: 4331 5945 6155 4331
Size: 1614 210 6098
Fragments arranged by size:
6098 1614 210
SbfI CC_TGCA'GG
Cuts at: 4787 4969 4787
Size: 182 7740
ScaI AGT'ACT
Cuts at: 187 1942 7439 187
Size: 1755 5497 670
Fragments arranged by size:
5497 1755 670
SfcI C'TryA_G
Cuts at: 33 520 595 2556 3099 3347 4056 4288 4421
Size: 487 75 1961 543 248 709 232 133
Cuts at: 4421 4703 4783 4797 4965 5650 6643 6834 33
Size: 282 80 14 168 685 993 191 1121
Fragments arranged by size:
1961 1121 993 709 685 543 487 282
248 232 191 168 133 80 75 14
SfiI GGCCn_nnn'nGGCC
Cuts at: 1843 1963 3472 1843
Size: 120 1509 6293
Fragments arranged by size:
6293 1509 120
SfoI GGC'GCC
Cuts at: 325 5354 5602 325
Size: 5029 248 2645
Fragments arranged by size:
5029 2645 248
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 7922
SgrAI Cr'CCGG_yG
Cuts at: 1292 1292
Size: 7922
SmaI CCC'GGG
Cuts at: 1230 2252 2503 4944 1230
Size: 1022 251 2441 4208
Fragments arranged by size:
4208 2441 1022 251
SmlI C'TyrA_G
Cuts at: 898 1599 3675 4381 5077 5992 6484 6746 898
Size: 701 2076 706 696 915 492 262 2074
Fragments arranged by size:
2076 2074 915 706 701 696 492 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 7922
SphI G_CATG'C
Cuts at: 4975 5475 4975
Size: 500 7422
SrfI GCCC'GGGC
Cuts at: 2252 2503 2252
Size: 251 7671
StuI AGG'CCT
Cuts at: 259 3211 3455 3475 3673 3838 259
Size: 2952 244 20 198 165 4343
Fragments arranged by size:
4343 2952 244 198 165 20
StyI C'CwwG_G
Cuts at: 341 718 741 1076 1790 1841 2445 2786 2966
Size: 377 23 335 714 51 604 341 180
Cuts at: 2966 3320 4188 4643 4871 5056 5410 341
Size: 354 868 455 228 185 354 2853
Fragments arranged by size:
2853 868 714 604 455 377 354 354
341 335 228 185 180 51 23
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 283 633 2040 2077 2288 2468 2509 2599 2745
Size: 350 1407 37 211 180 41 90 146
Cuts at: 2745 3001 3156 3634 4207 4277 4281 4828 5505
Size: 256 155 478 573 70 4 547 677
Cuts at: 5505 5638 6097 6306 283
Size: 133 459 209 1899
Fragments arranged by size:
1899 1407 677 573 547 478 459 350
256 211 209 180 155 146 133 90
70 41 37 4
TatI w'GTAC_w
Cuts at: 185 534 599 1940 7437 185
Size: 349 65 1341 5497 670
Fragments arranged by size:
5497 1341 670 349 65
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 262 294 2013 2045 3756 3788 4462 4494 4585
Size: 32 1719 32 1711 32 674 32 91
Cuts at: 4585 4617 7439 7471 262
Size: 32 2822 32 713
Fragments arranged by size:
2822 1719 1711 713 674 91 32 32
32 32 32 32
Tth111I GACn'n_nGTC
Cuts at: 4749 5719 4749
Size: 970 6952
XbaI T'CTAG_A
Cuts at: 4953 4953
Size: 7922
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 1371 3531 4828 5417 1371
Size: 2160 1297 589 3876
Fragments arranged by size:
3876 2160 1297 589
XhoI C'TCGA_G
Cuts at: 898 898
Size: 7922
XmaI C'CCGG_G
Cuts at: 1228 2250 2501 4942 1228
Size: 1022 251 2441 4208
Fragments arranged by size:
4208 2441 1022 251
ZraI GAC'GTC
Cuts at: 1134 1950 1134
Size: 816 7106
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AflIII AgeI
AleI AlfI AlwNI ApaI ApaLI ApoI AseI AsiSI
AvaI AvrII BamHI BanI BanII BbeI BbsI BbvCI
BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI
BmrI BmtI BplI BpmI Bpu10I BpuEI BsaI BsaAI
BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI
BsiHKAI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI
BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI
BstYI Bsu36I BtgI BtgZI BtsI CspCI DraI DraIII
DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI
EcoO109I EcoRI FalI FspI HaeII Hin4I HincII KasI
KpnI MluI MmeI MscI MslI MspA1I NaeI NarI
NcoI NgoMIV NheI NotI NspI PasI PciI PflMI
PfoI PmeI PpiI PpuMI PspOMI PspXI PstI PvuI
PvuII RsrII SacI SalI SanDI SapI SbfI ScaI
SfcI SfiI SfoI SgfI SgrAI SmaI SmlI SpeI
SphI SrfI StuI StyI TaqII TatI TstI Tth111I
XbaI XcmI XhoI XmaI ZraI
Enzymes that do not cut:
AfeI AflII AhdI AloI AscI BaeI BfrBI BsiWI
BsmI BstZ17I ClaI EcoRV FseI FspAI HindIII HpaI
MfeI NdeI NruI NsiI PacI PmlI PshAI PsiI
PsrI SacII SexAI SnaBI SspI SwaI XmnI