Restriction map for
pF1KE0079
(Circular) (Six-base) MAPSORT of: pF1KE0079.seq Check: 3563 from: 1 to: 4961
pF1KE0079 4961 bp
With 182 enzymes: SgfI *
April 24, 2009 13:10 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 171 1618 1710 171
Size: 1447 92 3422
Fragments arranged by size:
3422 1447 92
AccI GT'mk_AC
Cuts at: 1754 1999 1754
Size: 245 4716
Acc65I G'GTAC_C
Cuts at: 458 1977 458
Size: 1519 3442
AclI AA'CG_TT
Cuts at: 4801 4801
Size: 4961
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1110 1568 2786 3218 3965 4233 1110
Size: 458 1218 432 747 268 1838
Fragments arranged by size:
1838 1218 747 458 432 268
AflIII A'CryG_T
Cuts at: 272 1039 2313 3417 4394 272
Size: 767 1274 1104 977 839
Fragments arranged by size:
1274 1104 977 839 767
AgeI A'CCGG_T
Cuts at: 3349 3349
Size: 4961
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1458 1458
Size: 4961
AlwNI CAG_nnn'CTG
Cuts at: 620 1188 2266 3833 620
Size: 568 1078 1567 1748
Fragments arranged by size:
1748 1567 1078 568
ApaLI G'TGCA_C
Cuts at: 447 3731 447
Size: 3284 1677
ApoI r'AATT_y
Cuts at: 1965 4314 4570 1965
Size: 2349 256 2356
Fragments arranged by size:
2356 2349 256
AscI GG'CGCG_CC
Cuts at: 1834 1834
Size: 4961
AseI AT'TA_AT
Cuts at: 20 20
Size: 4961
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 4961
AvaI C'yCGr_G
Cuts at: 1585 1891 1920 1981 1585
Size: 306 29 61 4565
Fragments arranged by size:
4565 306 61 29
BamHI G'GATC_C
Cuts at: 1986 1986
Size: 4961
BanI G'GyrC_C
Cuts at: 128 458 520 1630 1977 2391 2639 2674 128
Size: 330 62 1110 347 414 248 35 2415
Fragments arranged by size:
2415 1110 414 347 330 248 62 35
BanII G_rGCy'C
Cuts at: 1328 1802 1975 3005 1328
Size: 474 173 1030 3284
Fragments arranged by size:
3284 1030 474 173
BbeI G_GCGC'C
Cuts at: 132 524 2395 2643 132
Size: 392 1871 248 2450
Fragments arranged by size:
2450 1871 392 248
BbsI GAAGACnn'nnnn_
Cuts at: 269 991 1124 269
Size: 722 133 4106
Fragments arranged by size:
4106 722 133
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1911 1945 1911
Size: 34 4927
BciVI GTATCCnnnnn_n'
Cuts at: 2859 3620 4961 2859
Size: 761 1341 2859
Fragments arranged by size:
2859 1341 761
BclI T'GATC_A
Cuts at: 2481 2481
Size: 4961
BglI GCCn_nnn'nGGC
Cuts at: 403 575 784 403
Size: 172 209 4580
Fragments arranged by size:
4580 209 172
BglII A'GATC_T
Cuts at: 2476 4513 2476
Size: 2037 2924
BlpI GC'TnA_GC
Cuts at: 1617 2073 1617
Size: 456 4505
Bme1580I G_kGCm'C
Cuts at: 451 1039 2586 2679 3735 451
Size: 588 1547 93 1056 1677
Fragments arranged by size:
1677 1547 1056 588 93
BmrI ACTGGGnnnn_n'
Cuts at: 1416 1440 1512 2173 2254 2589 4733 1416
Size: 24 72 661 81 335 2144 1644
Fragments arranged by size:
2144 1644 661 335 81 72 24
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 1935 3328 1935
Size: 1393 3568
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 486 997 1261 1695 486
Size: 511 264 434 3752
Fragments arranged by size:
3752 511 434 264
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2137 3016 3508 3806 2137
Size: 879 492 298 3292
Fragments arranged by size:
3292 879 492 298
BsaI GGTCTCn'nnnn_
Cuts at: 1571 1819 4665 1571
Size: 248 2846 1867
Fragments arranged by size:
2846 1867 248
BsaAI yAC'GTr
Cuts at: 2314 2314
Size: 4961
BsaBI GATnn'nnATC
Cuts at: 6 2475 4512 6
Size: 2469 2037 455
Fragments arranged by size:
2469 2037 455
BsaHI Gr'CG_yC
Cuts at: 129 521 2392 2640 3342 4838 129
Size: 392 1871 248 702 1496 252
Fragments arranged by size:
1871 1496 702 392 252 248
BsaWI w'CCGG_w
Cuts at: 2150 2671 3349 3623 3770 4181 2150
Size: 521 678 274 147 411 2930
Fragments arranged by size:
2930 678 521 411 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1150 1180 1681 1711 1150
Size: 30 501 30 4400
Fragments arranged by size:
4400 501 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 191 994 1837 191
Size: 803 843 3315
Fragments arranged by size:
3315 843 803
BseYI C'CCAG_C
Cuts at: 454 524 1169 1420 2386 3144 3721 4217 454
Size: 70 645 251 966 758 577 496 1198
Fragments arranged by size:
1198 966 758 645 577 496 251 70
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1302 1353 1428 1837 1951 1302
Size: 51 75 409 114 4312
Fragments arranged by size:
4312 409 114 75 51
BsiEI CG_ry'CG
Cuts at: 104 2503 2549 3757 104
Size: 2399 46 1208 1308
Fragments arranged by size:
2399 1308 1208 46
BsiHKAI G_wGCw'C
Cuts at: 451 1294 1975 2753 2943 3735 451
Size: 843 681 778 190 792 1677
Fragments arranged by size:
1677 843 792 778 681 190
Bsp1286I G_dGCh'C
Cuts at: 451 1039 1294 1328 1802 1975 2586 2679 2753
Size: 588 255 34 474 173 611 93 74
Cuts at: 2753 2943 3005 3735 451
Size: 190 62 730 1677
Fragments arranged by size:
1677 730 611 588 474 255 190 173
93 74 62 34
BspEI T'CCGG_A
Cuts at: 4181 4181
Size: 4961
BspHI T'CATG_A
Cuts at: 4137 4137
Size: 4961
BspMI ACCTGCnnnn'nnnn_
Cuts at: 171 1618 1710 2011 2225 2527 2908 171
Size: 1447 92 301 214 302 381 2224
Fragments arranged by size:
2224 1447 381 302 301 214 92
BsrBI CCG'CTC
Cuts at: 50 3253 3307 4791 4956 50
Size: 3203 54 1484 165 55
Fragments arranged by size:
3203 1484 165 55 54
BsrDI GCAATG_nn'
Cuts at: 1395 2873 1395
Size: 1478 3483
BsrFI r'CCGG_y
Cuts at: 415 1926 2959 3140 3349 415
Size: 1511 1033 181 209 2027
Fragments arranged by size:
2027 1511 1033 209 181
BssHII G'CGCG_C
Cuts at: 117 161 506 1834 117
Size: 44 345 1328 3244
Fragments arranged by size:
3244 1328 345 44
BssSI C'ACGA_G
Cuts at: 1486 3232 3590 1486
Size: 1746 358 2857
Fragments arranged by size:
2857 1746 358
BstAPI GCAn_nnn'nTGC
Cuts at: 534 566 1451 2338 534
Size: 32 885 887 3157
Fragments arranged by size:
3157 887 885 32
BstBI TT'CG_AA
Cuts at: 3322 3322
Size: 4961
BstYI r'GATC_y
Cuts at: 1 1986 2453 2476 2811 3057 4069 4155 4167
Size: 1985 467 23 335 246 1012 86 12
Cuts at: 4167 4513 1
Size: 346 449
Fragments arranged by size:
1985 1012 467 449 346 335 246 86
23 12
Bsu36I CC'TnA_GG
Cuts at: 897 897
Size: 4961
BtgI C'CryG_G
Cuts at: 107 407 602 1802 107
Size: 300 195 1200 3266
Fragments arranged by size:
3266 1200 300 195
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 2911 3092 3255 2911
Size: 181 163 4617
Fragments arranged by size:
4617 181 163
BtsI GCAGTG_nn'
Cuts at: 2204 2204
Size: 4961
DraI TTT'AAA
Cuts at: 1960 1960
Size: 4961
DraIII CAC_nnn'GTG
Cuts at: 503 557 503
Size: 54 4907
DrdI GACnn_nn'nnGTC
Cuts at: 910 1353 2667 3525 910
Size: 443 1314 858 2346
Fragments arranged by size:
2346 1314 858 443
EaeI y'GGCC_r
Cuts at: 404 870 1392 1656 2546 2720 3111 3138 4483
Size: 466 522 264 890 174 391 27 1345
Cuts at: 4483 404
Size: 882
Fragments arranged by size:
1345 890 882 522 466 391 264 174
27
EagI C'GGCC_G
Cuts at: 2546 2546
Size: 4961
EarI CTCTTCn'nnn_
Cuts at: 769 2984 3194 769
Size: 2215 210 2536
Fragments arranged by size:
2536 2215 210
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 3169 3479 3625 4771 3169
Size: 310 146 1146 3359
Fragments arranged by size:
3359 1146 310 146
EcoICRI GAG'CTC
Cuts at: 1973 1973
Size: 4961
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 486 997 1110 1261 1568 1695 2786 3218 3965
Size: 511 113 151 307 127 1091 432 747
Cuts at: 3965 4233 486
Size: 268 1214
Fragments arranged by size:
1214 1091 747 511 432 307 268 151
127 113
EcoO109I rG'GnC_Cy
Cuts at: 396 1163 2100 396
Size: 767 937 3257
Fragments arranged by size:
3257 937 767
EcoRI G'AATT_C
Cuts at: 1965 1965
Size: 4961
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2980 2980
Size: 4961
FspI TGC'GCA
Cuts at: 2742 2742
Size: 4961
HaeII r_GCGC'y
Cuts at: 132 441 524 2395 2643 3665 4644 132
Size: 309 83 1871 248 1022 979 449
Fragments arranged by size:
1871 1022 979 449 309 248 83
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 186 218 877 909 1150 1182 2803 2833 2835
Size: 32 659 32 241 32 1621 30 2
Cuts at: 2835 2865 186
Size: 30 2282
Fragments arranged by size:
2282 1621 659 241 32 32 32 30
30 2
HincII GTy'rAC
Cuts at: 2000 2000
Size: 4961
KasI G'GCGC_C
Cuts at: 128 520 2391 2639 128
Size: 392 1871 248 2450
Fragments arranged by size:
2450 1871 392 248
KpnI G_GTAC'C
Cuts at: 462 1981 462
Size: 1519 3442
MluI A'CGCG_T
Cuts at: 4394 4394
Size: 4961
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 3632 3816 3632
Size: 184 4777
MscI TGG'CCA
Cuts at: 1394 1658 2722 1394
Size: 264 1064 3633
Fragments arranged by size:
3633 1064 264
MslI CAynn'nnrTG
Cuts at: 1747 2950 3077 1747
Size: 1203 127 3631
Fragments arranged by size:
3631 1203 127
MspA1I CmG'CkG
Cuts at: 138 1715 2072 2386 2746 3759 4004 138
Size: 1577 357 314 360 1013 245 1095
Fragments arranged by size:
1577 1095 1013 360 357 314 245
NaeI GCC'GGC
Cuts at: 417 3142 417
Size: 2725 2236
NarI GG'CG_CC
Cuts at: 129 521 2392 2640 129
Size: 392 1871 248 2450
Fragments arranged by size:
2450 1871 392 248
NcoI C'CATG_G
Cuts at: 107 1802 107
Size: 1695 3266
NgoMIV G'CCGG_C
Cuts at: 415 3140 415
Size: 2725 2236
NspI r_CATG'y
Cuts at: 276 953 1043 2014 2514 3421 276
Size: 677 90 971 500 907 1816
Fragments arranged by size:
1816 971 907 677 500 90
PciI A'CATG_T
Cuts at: 272 1039 3417 272
Size: 767 2378 1816
Fragments arranged by size:
2378 1816 767
PflMI CCAn_nnn'nTGG
Cuts at: 1504 1504
Size: 4961
PfoI T'CCnGG_A
Cuts at: 1871 1871
Size: 4961
PmeI GTTT'AAAC
Cuts at: 1960 1960
Size: 4961
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4100 4132 4100
Size: 32 4929
PspXI vC'TCGA_Gb
Cuts at: 1920 1920
Size: 4961
PstI C_TGCA'G
Cuts at: 182 2008 2693 182
Size: 1826 685 2450
Fragments arranged by size:
2450 1826 685
PvuI CG_AT'CG
Cuts at: 104 104
Size: 4961
PvuII CAG'CTG
Cuts at: 2386 2746 2386
Size: 360 4601
RsrII CG'GwC_CG
Cuts at: 3156 3156
Size: 4961
SacI G_AGCT'C
Cuts at: 1975 1975
Size: 4961
SalI G'TCGA_C
Cuts at: 1998 1998
Size: 4961
SapI GCTCTTCn'nnn_
Cuts at: 2984 3194 2984
Size: 210 4751
SbfI CC_TGCA'GG
Cuts at: 2008 2008
Size: 4961
ScaI AGT'ACT
Cuts at: 4478 4478
Size: 4961
SfcI C'TryA_G
Cuts at: 33 178 810 2004 2689 3682 3873 33
Size: 145 632 1194 685 993 191 1121
Fragments arranged by size:
1194 1121 993 685 632 191 145
SfiI GGCCn_nnn'nGGCC
Cuts at: 403 403
Size: 4961
SfoI GGC'GCC
Cuts at: 130 522 2393 2641 130
Size: 392 1871 248 2450
Fragments arranged by size:
2450 1871 392 248
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 4961
SmaI CCC'GGG
Cuts at: 1983 1983
Size: 4961
SmlI C'TyrA_G
Cuts at: 1920 2116 3031 3523 3785 1920
Size: 196 915 492 262 3096
Fragments arranged by size:
3096 915 492 262 196
SpeI A'CTAG_T
Cuts at: 67 67
Size: 4961
SphI G_CATG'C
Cuts at: 2014 2514 2014
Size: 500 4461
StuI AGG'CCT
Cuts at: 1918 1918
Size: 4961
StyI C'CwwG_G
Cuts at: 107 639 1802 1949 2095 2449 107
Size: 532 1163 147 146 354 2619
Fragments arranged by size:
2619 1163 532 354 147 146
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 1120 2544 2677 3136 3345 1120
Size: 1424 133 459 209 2736
Fragments arranged by size:
2736 1424 459 209 133
TatI w'GTAC_w
Cuts at: 269 916 4476 269
Size: 647 3560 754
Fragments arranged by size:
3560 754 647
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 466 498 541 573 4478 4510 466
Size: 32 43 32 3905 32 917
Fragments arranged by size:
3905 917 43 32 32 32
Tth111I GACn'n_nGTC
Cuts at: 2758 2758
Size: 4961
XbaI T'CTAG_A
Cuts at: 1992 1992
Size: 4961
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 1525 1588 2456 1525
Size: 63 868 4030
Fragments arranged by size:
4030 868 63
XhoI C'TCGA_G
Cuts at: 1920 1920
Size: 4961
XmaI C'CCGG_G
Cuts at: 1981 1981
Size: 4961
Enzymes that do cut:
AarI AccI Acc65I AclI AcuI AflIII AgeI AlfI
AlwNI ApaLI ApoI AscI AseI AsiSI AvaI BamHI
BanI BanII BbeI BbsI BcgI BciVI BclI BglI
BglII BlpI Bme1580I BmrI BplI BpmI BpuEI BsaI
BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI
BsiEI BsiHKAI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI
BsrFI BssHII BssSI BstAPI BstBI BstYI Bsu36I BtgI
BtgZI BtsI DraI DraIII DrdI EaeI EagI EarI
EciI EcoICRI Eco57MI EcoO109I EcoRI FalI FspI HaeII
Hin4I HincII KasI KpnI MluI MmeI MscI MslI
MspA1I NaeI NarI NcoI NgoMIV NspI PciI PflMI
PfoI PmeI PpiI PspXI PstI PvuI PvuII RsrII
SacI SalI SapI SbfI ScaI SfcI SfiI SfoI
SgfI SmaI SmlI SpeI SphI StuI StyI TaqII
TatI TstI Tth111I XbaI XcmI XhoI XmaI
Enzymes that do not cut:
AatII AfeI AflII AhdI AleI AloI ApaI AvrII
BaeI BbvCI BfrBI BmgBI BmtI Bpu10I BsiWI BsmI
BsmBI BsrGI BstEII BstXI BstZ17I ClaI CspCI EcoNI
EcoRV FseI FspAI HindIII HpaI MfeI NdeI NheI
NotI NruI NsiI PacI PasI PmlI PpuMI PshAI
PsiI PspOMI PsrI SacII SanDI SexAI SgrAI SnaBI
SrfI SspI SwaI XmnI ZraI