Restriction map for
pF1KE0085
(Circular) (Six-base) MAPSORT of: pF1KE0085.seq Check: 4320 from: 1 to: 7364
pF1KE0085 7364 bp
With 182 enzymes: SgfI *
April 24, 2009 13:10 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 3879 3879
Size: 7364
AccI GT'mk_AC
Cuts at: 4402 4402
Size: 7364
Acc65I G'GTAC_C
Cuts at: 4380 4380
Size: 7364
AclI AA'CG_TT
Cuts at: 2819 7204 2819
Size: 4385 2979
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 568 1286 2642 2768 2824 4052 5189 5621 6368
Size: 718 1356 126 56 1228 1137 432 747
Cuts at: 6368 6636 568
Size: 268 1296
Fragments arranged by size:
1356 1296 1228 1137 747 718 432 268
126 56
AflII C'TTAA_G
Cuts at: 534 534
Size: 7364
AflIII A'CryG_T
Cuts at: 4716 5820 6797 4716
Size: 1104 977 5283
Fragments arranged by size:
5283 1104 977
AgeI A'CCGG_T
Cuts at: 5752 5752
Size: 7364
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 857 857
Size: 7364
AlwNI CAG_nnn'CTG
Cuts at: 1930 4669 6236 1930
Size: 2739 1567 3058
Fragments arranged by size:
3058 2739 1567
ApaI G_GGCC'C
Cuts at: 372 372
Size: 7364
ApaLI G'TGCA_C
Cuts at: 6134 6134
Size: 7364
ApoI r'AATT_y
Cuts at: 307 612 880 990 1358 1437 1531 1823 2076
Size: 305 268 110 368 79 94 292 253
Cuts at: 2076 2348 2378 2663 4368 6717 6973 307
Size: 272 30 285 1705 2349 256 698
Fragments arranged by size:
2349 1705 698 368 305 292 285 272
268 256 253 110 94 79 30
AseI AT'TA_AT
Cuts at: 20 2330 20
Size: 2310 5054
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 7364
AvaI C'yCGr_G
Cuts at: 160 183 371 404 3881 4384 160
Size: 23 188 33 3477 503 3140
Fragments arranged by size:
3477 3140 503 188 33 23
BamHI G'GATC_C
Cuts at: 4389 4389
Size: 7364
BanI G'GyrC_C
Cuts at: 233 4380 4794 5042 5077 233
Size: 4147 414 248 35 2520
Fragments arranged by size:
4147 2520 414 248 35
BanII G_rGCy'C
Cuts at: 171 184 372 3482 4378 5408 171
Size: 13 188 3110 896 1030 2127
Fragments arranged by size:
3110 2127 1030 896 188 13
BbeI G_GCGC'C
Cuts at: 237 4798 5046 237
Size: 4561 248 2555
Fragments arranged by size:
4561 2555 248
BbsI GAAGACnn'nnnn_
Cuts at: 1748 2788 3470 4196 1748
Size: 1040 682 726 4916
Fragments arranged by size:
4916 1040 726 682
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 295 329 295
Size: 34 7330
BciVI GTATCCnnnnn_n'
Cuts at: 4260 5262 6023 7364 4260
Size: 1002 761 1341 4260
Fragments arranged by size:
4260 1341 1002 761
BclI T'GATC_A
Cuts at: 4884 4884
Size: 7364
BglII A'GATC_T
Cuts at: 4879 6916 4879
Size: 2037 5327
BlpI GC'TnA_GC
Cuts at: 4476 4476
Size: 7364
Bme1580I G_kGCm'C
Cuts at: 372 4989 5082 6138 372
Size: 4617 93 1056 1598
Fragments arranged by size:
4617 1598 1056 93
BmrI ACTGGGnnnn_n'
Cuts at: 1656 4048 4576 4657 4992 7136 1656
Size: 2392 528 81 335 2144 1884
Fragments arranged by size:
2392 2144 1884 528 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 5731 5731
Size: 7364
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2145 3340 3640 3997 2145
Size: 1195 300 357 5512
Fragments arranged by size:
5512 1195 357 300
Bpu10I CC'TnA_GC
Cuts at: 3539 4262 3539
Size: 723 6641
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 955 998 2233 3322 4540 5419 5911 6209 955
Size: 43 1235 1089 1218 879 492 298 2110
Fragments arranged by size:
2110 1235 1218 1089 879 492 298 43
BsaI GGTCTCn'nnnn_
Cuts at: 440 7068 440
Size: 6628 736
BsaAI yAC'GTr
Cuts at: 2047 4057 4717 2047
Size: 2010 660 4694
Fragments arranged by size:
4694 2010 660
BsaBI GATnn'nnATC
Cuts at: 6 1976 4878 6915 6
Size: 1970 2902 2037 455
Fragments arranged by size:
2902 2037 1970 455
BsaHI Gr'CG_yC
Cuts at: 234 4795 5043 5745 7241 234
Size: 4561 248 702 1496 357
Fragments arranged by size:
4561 1496 702 357 248
BsaWI w'CCGG_w
Cuts at: 4553 5074 5752 6026 6173 6584 4553
Size: 521 678 274 147 411 5333
Fragments arranged by size:
5333 678 521 411 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 4340 4370 4340
Size: 30 7334
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 2149 3004 3593 2149
Size: 855 589 5920
Fragments arranged by size:
5920 855 589
BseYI C'CCAG_C
Cuts at: 442 4789 5547 6124 6620 442
Size: 4347 758 577 496 1186
Fragments arranged by size:
4347 1186 758 577 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 2993 3913 2993
Size: 920 6444
BsiEI CG_ry'CG
Cuts at: 104 4906 4952 6160 104
Size: 4802 46 1208 1308
Fragments arranged by size:
4802 1308 1208 46
BsiHKAI G_wGCw'C
Cuts at: 3482 3575 4378 5156 5346 6138 3482
Size: 93 803 778 190 792 4708
Fragments arranged by size:
4708 803 792 778 190 93
BsmI GAATG_Cn'
Cuts at: 846 3451 4085 846
Size: 2605 634 4125
Fragments arranged by size:
4125 2605 634
Bsp1286I G_dGCh'C
Cuts at: 171 184 372 3482 3575 4378 4989 5082 5156
Size: 13 188 3110 93 803 611 93 74
Cuts at: 5156 5346 5408 6138 171
Size: 190 62 730 1397
Fragments arranged by size:
3110 1397 803 730 611 190 188 93
93 74 62 13
BspEI T'CCGG_A
Cuts at: 6584 6584
Size: 7364
BspHI T'CATG_A
Cuts at: 6540 6540
Size: 7364
BspMI ACCTGCnnnn'nnnn_
Cuts at: 3879 4414 4628 4930 5311 3879
Size: 535 214 302 381 5932
Fragments arranged by size:
5932 535 381 302 214
BsrBI CCG'CTC
Cuts at: 50 5656 5710 7194 7359 50
Size: 5606 54 1484 165 55
Fragments arranged by size:
5606 1484 165 55 54
BsrDI GCAATG_nn'
Cuts at: 3164 3827 5276 3164
Size: 663 1449 5252
Fragments arranged by size:
5252 1449 663
BsrFI r'CCGG_y
Cuts at: 5362 5543 5752 5362
Size: 181 209 6974
Fragments arranged by size:
6974 209 181
BssHII G'CGCG_C
Cuts at: 188 416 188
Size: 228 7136
BssSI C'ACGA_G
Cuts at: 5635 5993 5635
Size: 358 7006
BstAPI GCAn_nnn'nTGC
Cuts at: 526 1757 2266 4741 526
Size: 1231 509 2475 3149
Fragments arranged by size:
3149 2475 1231 509
BstBI TT'CG_AA
Cuts at: 305 5725 305
Size: 5420 1944
BstXI CCAn_nnnn'nTGG
Cuts at: 2169 3858 2169
Size: 1689 5675
BstYI r'GATC_y
Cuts at: 1 3423 4389 4856 4879 5214 5460 6472 6558
Size: 3422 966 467 23 335 246 1012 86
Cuts at: 6558 6570 6916 1
Size: 12 346 449
Fragments arranged by size:
3422 1012 966 467 449 346 335 246
86 23 12
BtgI C'CryG_G
Cuts at: 107 295 107
Size: 188 7176
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 5314 5495 5658 5314
Size: 181 163 7020
Fragments arranged by size:
7020 181 163
BtsI GCAGTG_nn'
Cuts at: 270 1436 3056 4607 270
Size: 1166 1620 1551 3027
Fragments arranged by size:
3027 1620 1551 1166
DraI TTT'AAA
Cuts at: 1695 2460 2597 4363 1695
Size: 765 137 1766 4696
Fragments arranged by size:
4696 1766 765 137
DrdI GACnn_nn'nnGTC
Cuts at: 5070 5928 5070
Size: 858 6506
EaeI y'GGCC_r
Cuts at: 4949 5123 5514 5541 6886 4949
Size: 174 391 27 1345 5427
Fragments arranged by size:
5427 1345 391 174 27
EagI C'GGCC_G
Cuts at: 4949 4949
Size: 7364
EarI CTCTTCn'nnn_
Cuts at: 497 578 1193 2744 2843 3092 5387 5597 497
Size: 81 615 1551 99 249 2295 210 2264
Fragments arranged by size:
2295 2264 1551 615 249 210 99 81
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 5572 5882 6028 7174 5572
Size: 310 146 1146 5762
Fragments arranged by size:
5762 1146 310 146
EcoICRI GAG'CTC
Cuts at: 3480 4376 3480
Size: 896 6468
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 568 1286 2145 2642 2768 2824 3340 3640 3997
Size: 718 859 497 126 56 516 300 357
Cuts at: 3997 4052 5189 5621 6368 6636 568
Size: 55 1137 432 747 268 1296
Fragments arranged by size:
1296 1137 859 747 718 516 497 432
357 300 268 126 56 55
EcoO109I rG'GnC_Cy
Cuts at: 368 4503 368
Size: 4135 3229
EcoRI G'AATT_C
Cuts at: 307 4368 307
Size: 4061 3303
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 1146 3718 4354 5383 1146
Size: 2572 636 1029 3127
Fragments arranged by size:
3127 2572 1029 636
FspI TGC'GCA
Cuts at: 5145 5145
Size: 7364
HaeII r_GCGC'y
Cuts at: 237 4798 5046 6068 7047 237
Size: 4561 248 1022 979 554
Fragments arranged by size:
4561 1022 979 554 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1839 1871 2298 2330 2772 2804 3174 3206 3651
Size: 32 427 32 442 32 370 32 445
Cuts at: 3651 3683 4140 4172 5206 5236 5238 5268 1839
Size: 32 457 32 1034 30 2 30 3935
Fragments arranged by size:
3935 1034 457 445 442 427 370 32
32 32 32 32 32 30 30 2
HincII GTy'rAC
Cuts at: 1606 2094 3751 4403 1606
Size: 488 1657 652 4567
Fragments arranged by size:
4567 1657 652 488
HindIII A'AGCT_T
Cuts at: 1211 1801 1831 2150 2591 4130 1211
Size: 590 30 319 441 1539 4445
Fragments arranged by size:
4445 1539 590 441 319 30
HpaI GTT'AAC
Cuts at: 2094 2094
Size: 7364
KasI G'GCGC_C
Cuts at: 233 4794 5042 233
Size: 4561 248 2555
Fragments arranged by size:
4561 2555 248
KpnI G_GTAC'C
Cuts at: 4384 4384
Size: 7364
MfeI C'AATT_G
Cuts at: 2698 2698
Size: 7364
MluI A'CGCG_T
Cuts at: 6797 6797
Size: 7364
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1242 6035 6219 1242
Size: 4793 184 2387
Fragments arranged by size:
4793 2387 184
MscI TGG'CCA
Cuts at: 5125 5125
Size: 7364
MslI CAynn'nnrTG
Cuts at: 1429 5353 5480 1429
Size: 3924 127 3313
Fragments arranged by size:
3924 3313 127
MspA1I CmG'CkG
Cuts at: 297 2124 2223 2968 3007 3433 4475 4789 5149
Size: 1827 99 745 39 426 1042 314 360
Cuts at: 5149 6162 6407 297
Size: 1013 245 1254
Fragments arranged by size:
1827 1254 1042 1013 745 426 360 314
245 99 39
NaeI GCC'GGC
Cuts at: 5545 5545
Size: 7364
NarI GG'CG_CC
Cuts at: 234 4795 5043 234
Size: 4561 248 2555
Fragments arranged by size:
4561 2555 248
NcoI C'CATG_G
Cuts at: 107 107
Size: 7364
NdeI CA'TA_TG
Cuts at: 4139 4139
Size: 7364
NgoMIV G'CCGG_C
Cuts at: 5543 5543
Size: 7364
NspI r_CATG'y
Cuts at: 4417 4917 5824 4417
Size: 500 907 5957
Fragments arranged by size:
5957 907 500
PciI A'CATG_T
Cuts at: 5820 5820
Size: 7364
PflMI CCAn_nnn'nTGG
Cuts at: 1065 1065
Size: 7364
PfoI T'CCnGG_A
Cuts at: 3356 3356
Size: 7364
PmeI GTTT'AAAC
Cuts at: 4363 4363
Size: 7364
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 6503 6535 6503
Size: 32 7332
PsiI TTA'TAA
Cuts at: 988 3721 988
Size: 2733 4631
PspOMI G'GGCC_C
Cuts at: 368 368
Size: 7364
PspXI vC'TCGA_Gb
Cuts at: 404 404
Size: 7364
PstI C_TGCA'G
Cuts at: 4411 5096 4411
Size: 685 6679
PvuI CG_AT'CG
Cuts at: 104 104
Size: 7364
PvuII CAG'CTG
Cuts at: 2124 2223 2968 3007 3433 4789 5149 2124
Size: 99 745 39 426 1356 360 4339
Fragments arranged by size:
4339 1356 745 426 360 99 39
RsrII CG'GwC_CG
Cuts at: 5559 5559
Size: 7364
SacI G_AGCT'C
Cuts at: 3482 4378 3482
Size: 896 6468
SacII CC_GC'GG
Cuts at: 298 298
Size: 7364
SalI G'TCGA_C
Cuts at: 4401 4401
Size: 7364
SapI GCTCTTCn'nnn_
Cuts at: 1193 3092 5387 5597 1193
Size: 1899 2295 210 2960
Fragments arranged by size:
2960 2295 1899 210
SbfI CC_TGCA'GG
Cuts at: 4411 4411
Size: 7364
ScaI AGT'ACT
Cuts at: 3258 3299 3342 6881 3258
Size: 41 43 3539 3741
Fragments arranged by size:
3741 3539 43 41
SfcI C'TryA_G
Cuts at: 33 2827 3376 4407 5092 6085 6276 33
Size: 2794 549 1031 685 993 191 1121
Fragments arranged by size:
2794 1121 1031 993 685 549 191
SfoI GGC'GCC
Cuts at: 235 4796 5044 235
Size: 4561 248 2555
Fragments arranged by size:
4561 2555 248
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 7364
SmaI CCC'GGG
Cuts at: 162 373 3883 4386 162
Size: 211 3510 503 3140
Fragments arranged by size:
3510 3140 503 211
SmlI C'TyrA_G
Cuts at: 404 534 934 1013 2212 3301 4519 5434 5926
Size: 130 400 79 1199 1089 1218 915 492
Cuts at: 5926 6188 404
Size: 262 1580
Fragments arranged by size:
1580 1218 1199 1089 915 492 400 262
130 79
SnaBI TAC'GTA
Cuts at: 2047 2047
Size: 7364
SpeI A'CTAG_T
Cuts at: 67 2110 67
Size: 2043 5321
SphI G_CATG'C
Cuts at: 4417 4917 4417
Size: 500 6864
SspI AAT'ATT
Cuts at: 1277 2328 1277
Size: 1051 6313
StyI C'CwwG_G
Cuts at: 107 4498 4852 107
Size: 4391 354 2619
Fragments arranged by size:
4391 2619 354
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 445 4947 5080 5539 5748 445
Size: 4502 133 459 209 2061
Fragments arranged by size:
4502 2061 459 209 133
TatI w'GTAC_w
Cuts at: 3089 3256 3297 3340 3372 3824 3839 6879 3089
Size: 167 41 43 32 452 15 3040 3574
Fragments arranged by size:
3574 3040 452 167 43 41 32 15
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 4340 4372 6881 6913 4340
Size: 32 2509 32 4791
Fragments arranged by size:
4791 2509 32 32
Tth111I GACn'n_nGTC
Cuts at: 2478 5161 2478
Size: 2683 4681
XbaI T'CTAG_A
Cuts at: 1468 2316 4395 1468
Size: 848 2079 4437
Fragments arranged by size:
4437 2079 848
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 450 4859 450
Size: 4409 2955
XhoI C'TCGA_G
Cuts at: 404 404
Size: 7364
XmaI C'CCGG_G
Cuts at: 160 371 3881 4384 160
Size: 211 3510 503 3140
Fragments arranged by size:
3510 3140 503 211
XmnI GAAnn'nnTTC
Cuts at: 890 1256 3701 890
Size: 366 2445 4553
Fragments arranged by size:
4553 2445 366
Enzymes that do cut:
AarI AccI Acc65I AclI AcuI AflII AflIII AgeI
AlfI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI
BamHI BanI BanII BbeI BbsI BcgI BciVI BclI
BglII BlpI Bme1580I BmrI BplI BpmI Bpu10I BpuEI
BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI
BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI
BsrBI BsrDI BsrFI BssHII BssSI BstAPI BstBI BstXI
BstYI BtgI BtgZI BtsI DraI DrdI EaeI EagI
EarI EciI EcoICRI Eco57MI EcoO109I EcoRI FalI FspI
HaeII Hin4I HincII HindIII HpaI KasI KpnI MfeI
MluI MmeI MscI MslI MspA1I NaeI NarI NcoI
NdeI NgoMIV NspI PciI PflMI PfoI PmeI PpiI
PsiI PspOMI PspXI PstI PvuI PvuII RsrII SacI
SacII SalI SapI SbfI ScaI SfcI SfoI SgfI
SmaI SmlI SnaBI SpeI SphI SspI StyI TaqII
TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI
Enzymes that do not cut:
AatII AfeI AhdI AleI AloI AscI AvrII BaeI
BbvCI BfrBI BglI BmgBI BmtI BsiWI BsmBI BsrGI
BstEII BstZ17I Bsu36I ClaI CspCI DraIII EcoNI EcoRV
FseI FspAI NheI NotI NruI NsiI PacI PasI
PmlI PpuMI PshAI PsrI SanDI SexAI SfiI SgrAI
SrfI StuI SwaI ZraI