Restriction map for
pF1KE0087
(Circular) (Six-base) MAPSORT of: pF1KE0087.seq Check: 9886 from: 1 to: 5879
pF1KE0087 5879 bp
With 182 enzymes: SgfI *
April 24, 2009 13:10 ..
AccI GT'mk_AC
Cuts at: 2917 2917
Size: 5879
Acc65I G'GTAC_C
Cuts at: 2895 2895
Size: 5879
AclI AA'CG_TT
Cuts at: 5719 5719
Size: 5879
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 353 511 2555 3704 4136 4883 5151 353
Size: 158 2044 1149 432 747 268 1081
Fragments arranged by size:
2044 1149 1081 747 432 268 158
AflIII A'CryG_T
Cuts at: 230 1811 2179 3231 4335 5312 230
Size: 1581 368 1052 1104 977 797
Fragments arranged by size:
1581 1104 1052 977 797 368
AgeI A'CCGG_T
Cuts at: 4267 4267
Size: 5879
AlwNI CAG_nnn'CTG
Cuts at: 3184 4751 3184
Size: 1567 4312
ApaLI G'TGCA_C
Cuts at: 4649 4649
Size: 5879
ApoI r'AATT_y
Cuts at: 344 592 669 807 1044 1474 1745 2883 5232
Size: 248 77 138 237 430 271 1138 2349
Cuts at: 5232 5488 344
Size: 256 735
Fragments arranged by size:
2349 1138 735 430 271 256 248 237
138 77
AseI AT'TA_AT
Cuts at: 20 20
Size: 5879
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 5879
AvaI C'yCGr_G
Cuts at: 2899 2899
Size: 5879
BamHI G'GATC_C
Cuts at: 322 2904 322
Size: 2582 3297
BanI G'GyrC_C
Cuts at: 2895 3309 3557 3592 2895
Size: 414 248 35 5182
Fragments arranged by size:
5182 414 248 35
BanII G_rGCy'C
Cuts at: 1899 2893 3923 1899
Size: 994 1030 3855
Fragments arranged by size:
3855 1030 994
BbeI G_GCGC'C
Cuts at: 3313 3561 3313
Size: 248 5631
BbsI GAAGACnn'nnnn_
Cuts at: 144 183 517 1532 2201 2755 2826 144
Size: 39 334 1015 669 554 71 3197
Fragments arranged by size:
3197 1015 669 554 334 71 39
BciVI GTATCCnnnnn_n'
Cuts at: 1275 3777 4538 5879 1275
Size: 2502 761 1341 1275
Fragments arranged by size:
2502 1341 1275 761
BclI T'GATC_A
Cuts at: 2324 3399 2324
Size: 1075 4804
BfrBI ATG'CAT
Cuts at: 2857 2857
Size: 5879
BglII A'GATC_T
Cuts at: 3394 5431 3394
Size: 2037 3842
BlpI GC'TnA_GC
Cuts at: 1647 2991 1647
Size: 1344 4535
Bme1580I G_kGCm'C
Cuts at: 3504 3597 4653 3504
Size: 93 1056 4730
Fragments arranged by size:
4730 1056 93
BmgBI CAC'GTC
Cuts at: 1814 1814
Size: 5879
BmrI ACTGGGnnnn_n'
Cuts at: 3091 3172 3507 5651 3091
Size: 81 335 2144 3319
Fragments arranged by size:
3319 2144 335 81
BmtI G_CTAG'C
Cuts at: 2248 2248
Size: 5879
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4246 4246
Size: 5879
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 851 2214 851
Size: 1363 4516
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 3055 3934 4426 4724 3055
Size: 879 492 298 4210
Fragments arranged by size:
4210 879 492 298
BsaI GGTCTCn'nnnn_
Cuts at: 945 5583 945
Size: 4638 1241
BsaAI yAC'GTr
Cuts at: 3232 3232
Size: 5879
BsaBI GATnn'nnATC
Cuts at: 6 3393 5430 6
Size: 3387 2037 455
Fragments arranged by size:
3387 2037 455
BsaHI Gr'CG_yC
Cuts at: 3310 3558 4260 5756 3310
Size: 248 702 1496 3433
Fragments arranged by size:
3433 1496 702 248
BsaWI w'CCGG_w
Cuts at: 3068 3589 4267 4541 4688 5099 3068
Size: 521 678 274 147 411 3848
Fragments arranged by size:
3848 678 521 411 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 199 229 940 970 199
Size: 30 711 30 5108
Fragments arranged by size:
5108 711 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 220 2487 220
Size: 2267 3612
BseYI C'CCAG_C
Cuts at: 3304 4062 4639 5135 3304
Size: 758 577 496 4048
Fragments arranged by size:
4048 758 577 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 237 237
Size: 5879
BsiEI CG_ry'CG
Cuts at: 104 3421 3467 4675 104
Size: 3317 46 1208 1308
Fragments arranged by size:
3317 1308 1208 46
BsiHKAI G_wGCw'C
Cuts at: 1273 1648 1899 2355 2893 3671 3861 4653 1273
Size: 375 251 456 538 778 190 792 2499
Fragments arranged by size:
2499 792 778 538 456 375 251 190
BsmI GAATG_Cn'
Cuts at: 1000 1000
Size: 5879
Bsp1286I G_dGCh'C
Cuts at: 1273 1648 1899 2355 2893 3504 3597 3671 3861
Size: 375 251 456 538 611 93 74 190
Cuts at: 3861 3923 4653 1273
Size: 62 730 2499
Fragments arranged by size:
2499 730 611 538 456 375 251 190
93 74 62
BspEI T'CCGG_A
Cuts at: 5099 5099
Size: 5879
BspHI T'CATG_A
Cuts at: 5055 5055
Size: 5879
BspMI ACCTGCnnnn'nnnn_
Cuts at: 2929 3143 3445 3826 2929
Size: 214 302 381 4982
Fragments arranged by size:
4982 381 302 214
BsrBI CCG'CTC
Cuts at: 50 4171 4225 5709 5874 50
Size: 4121 54 1484 165 55
Fragments arranged by size:
4121 1484 165 55 54
BsrDI GCAATG_nn'
Cuts at: 425 652 1718 3791 425
Size: 227 1066 2073 2513
Fragments arranged by size:
2513 2073 1066 227
BsrFI r'CCGG_y
Cuts at: 3877 4058 4267 3877
Size: 181 209 5489
Fragments arranged by size:
5489 209 181
BsrGI T'GTAC_A
Cuts at: 546 1808 546
Size: 1262 4617
BssSI C'ACGA_G
Cuts at: 4150 4508 4150
Size: 358 5521
BstAPI GCAn_nnn'nTGC
Cuts at: 3256 3256
Size: 5879
BstBI TT'CG_AA
Cuts at: 4240 4240
Size: 5879
BstYI r'GATC_y
Cuts at: 1 322 2904 3371 3394 3729 3975 4987 5073
Size: 321 2582 467 23 335 246 1012 86
Cuts at: 5073 5085 5431 1
Size: 12 346 449
Fragments arranged by size:
2582 1012 467 449 346 335 321 246
86 23 12
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 3829 4010 4173 3829
Size: 181 163 5535
Fragments arranged by size:
5535 181 163
BtsI GCAGTG_nn'
Cuts at: 929 3122 929
Size: 2193 3686
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 590 625 1532 1567 590
Size: 35 907 35 4902
Fragments arranged by size:
4902 907 35 35
DraI TTT'AAA
Cuts at: 1482 1623 1803 2073 2643 2878 1482
Size: 141 180 270 570 235 4483
Fragments arranged by size:
4483 570 270 235 180 141
DrdI GACnn_nn'nnGTC
Cuts at: 3585 4443 3585
Size: 858 5021
EaeI y'GGCC_r
Cuts at: 3464 3638 4029 4056 5401 3464
Size: 174 391 27 1345 3942
Fragments arranged by size:
3942 1345 391 174 27
EagI C'GGCC_G
Cuts at: 3464 3464
Size: 5879
EarI CTCTTCn'nnn_
Cuts at: 1904 3902 4112 1904
Size: 1998 210 3671
Fragments arranged by size:
3671 1998 210
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 4087 4397 4543 5689 4087
Size: 310 146 1146 4277
Fragments arranged by size:
4277 1146 310 146
EcoICRI GAG'CTC
Cuts at: 1897 2891 1897
Size: 994 4885
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 353 511 851 2214 2555 3704 4136 4883 5151
Size: 158 340 1363 341 1149 432 747 268
Cuts at: 5151 353
Size: 1081
Fragments arranged by size:
1363 1149 1081 747 432 341 340 268
158
EcoO109I rG'GnC_Cy
Cuts at: 3018 3018
Size: 5879
EcoRI G'AATT_C
Cuts at: 1474 2883 1474
Size: 1409 4470
EcoRV GAT'ATC
Cuts at: 2153 2280 2291 2153
Size: 127 11 5741
Fragments arranged by size:
5741 127 11
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 483 1869 2560 3898 483
Size: 1386 691 1338 2464
Fragments arranged by size:
2464 1386 1338 691
FspI TGC'GCA
Cuts at: 3660 3660
Size: 5879
HaeII r_GCGC'y
Cuts at: 3313 3561 4583 5562 3313
Size: 248 1022 979 3630
Fragments arranged by size:
3630 1022 979 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 940 972 1393 1425 3721 3751 3753 3783 940
Size: 32 421 32 2296 30 2 30 3036
Fragments arranged by size:
3036 2296 421 32 32 30 30 2
HincII GTy'rAC
Cuts at: 986 2918 986
Size: 1932 3947
HindIII A'AGCT_T
Cuts at: 1616 1616
Size: 5879
KasI G'GCGC_C
Cuts at: 3309 3557 3309
Size: 248 5631
KpnI G_GTAC'C
Cuts at: 2899 2899
Size: 5879
MfeI C'AATT_G
Cuts at: 976 2315 976
Size: 1339 4540
MluI A'CGCG_T
Cuts at: 5312 5312
Size: 5879
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1273 1976 2317 2713 4550 4734 1273
Size: 703 341 396 1837 184 2418
Fragments arranged by size:
2418 1837 703 396 341 184
MscI TGG'CCA
Cuts at: 3640 3640
Size: 5879
MslI CAynn'nnrTG
Cuts at: 896 1149 1321 3868 3995 896
Size: 253 172 2547 127 2780
Fragments arranged by size:
2780 2547 253 172 127
MspA1I CmG'CkG
Cuts at: 2990 3304 3664 4677 4922 2990
Size: 314 360 1013 245 3947
Fragments arranged by size:
3947 1013 360 314 245
NaeI GCC'GGC
Cuts at: 4060 4060
Size: 5879
NarI GG'CG_CC
Cuts at: 3310 3558 3310
Size: 248 5631
NdeI CA'TA_TG
Cuts at: 297 297
Size: 5879
NgoMIV G'CCGG_C
Cuts at: 4058 4058
Size: 5879
NheI G'CTAG_C
Cuts at: 2244 2244
Size: 5879
NsiI A_TGCA'T
Cuts at: 2859 2859
Size: 5879
NspI r_CATG'y
Cuts at: 234 2553 2601 2932 3432 4339 234
Size: 2319 48 331 500 907 1774
Fragments arranged by size:
2319 1774 907 500 331 48
PasI CC'CwG_GG
Cuts at: 174 174
Size: 5879
PciI A'CATG_T
Cuts at: 230 4335 230
Size: 4105 1774
PflMI CCAn_nnn'nTGG
Cuts at: 965 965
Size: 5879
PfoI T'CCnGG_A
Cuts at: 839 839
Size: 5879
PmeI GTTT'AAAC
Cuts at: 2878 2878
Size: 5879
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 5018 5050 5018
Size: 32 5847
PshAI GACnn'nnGTC
Cuts at: 937 2317 937
Size: 1380 4499
PsiI TTA'TAA
Cuts at: 401 1084 401
Size: 683 5196
PsrI GAACnnnnnnTACnnnnnnn_nnnnn'
Cuts at: 1427 1459 1427
Size: 32 5847
PstI C_TGCA'G
Cuts at: 2926 3611 2926
Size: 685 5194
PvuI CG_AT'CG
Cuts at: 104 104
Size: 5879
PvuII CAG'CTG
Cuts at: 3304 3664 3304
Size: 360 5519
RsrII CG'GwC_CG
Cuts at: 4074 4074
Size: 5879
SacI G_AGCT'C
Cuts at: 1899 2893 1899
Size: 994 4885
SalI G'TCGA_C
Cuts at: 2916 2916
Size: 5879
SapI GCTCTTCn'nnn_
Cuts at: 1904 3902 4112 1904
Size: 1998 210 3671
Fragments arranged by size:
3671 1998 210
SbfI CC_TGCA'GG
Cuts at: 2926 2926
Size: 5879
ScaI AGT'ACT
Cuts at: 2559 5396 2559
Size: 2837 3042
SfcI C'TryA_G
Cuts at: 33 824 989 1677 2922 3607 4600 4791 33
Size: 791 165 688 1245 685 993 191 1121
Fragments arranged by size:
1245 1121 993 791 688 685 191 165
SfoI GGC'GCC
Cuts at: 3311 3559 3311
Size: 248 5631
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 5879
SmaI CCC'GGG
Cuts at: 2901 2901
Size: 5879
SmlI C'TyrA_G
Cuts at: 3034 3949 4441 4703 3034
Size: 915 492 262 4210
Fragments arranged by size:
4210 915 492 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 5879
SphI G_CATG'C
Cuts at: 2553 2601 2932 3432 2553
Size: 48 331 500 5000
Fragments arranged by size:
5000 500 331 48
StyI C'CwwG_G
Cuts at: 1693 3013 3367 1693
Size: 1320 354 4205
Fragments arranged by size:
4205 1320 354
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 622 1106 3462 3595 4054 4263 622
Size: 484 2356 133 459 209 2238
Fragments arranged by size:
2356 2238 484 459 209 133
TatI w'GTAC_w
Cuts at: 546 1808 2182 2557 5394 546
Size: 1262 374 375 2837 1031
Fragments arranged by size:
2837 1262 1031 375 374
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 199 231 2331 2363 5396 5428 199
Size: 32 2100 32 3033 32 650
Fragments arranged by size:
3033 2100 650 32 32 32
Tth111I GACn'n_nGTC
Cuts at: 3676 3676
Size: 5879
XbaI T'CTAG_A
Cuts at: 1584 2458 2910 1584
Size: 874 452 4553
Fragments arranged by size:
4553 874 452
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 3374 3374
Size: 5879
XmaI C'CCGG_G
Cuts at: 2899 2899
Size: 5879
Enzymes that do cut:
AccI Acc65I AclI AcuI AflIII AgeI AlwNI ApaLI
ApoI AseI AsiSI AvaI BamHI BanI BanII BbeI
BbsI BciVI BclI BfrBI BglII BlpI Bme1580I BmgBI
BmrI BmtI BplI BpmI BpuEI BsaI BsaAI BsaBI
BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI
BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI
BsrGI BssSI BstAPI BstBI BstYI BtgZI BtsI CspCI
DraI DrdI EaeI EagI EarI EciI EcoICRI Eco57MI
EcoO109I EcoRI EcoRV FalI FspI HaeII Hin4I HincII
HindIII KasI KpnI MfeI MluI MmeI MscI MslI
MspA1I NaeI NarI NdeI NgoMIV NheI NsiI NspI
PasI PciI PflMI PfoI PmeI PpiI PshAI PsiI
PsrI PstI PvuI PvuII RsrII SacI SalI SapI
SbfI ScaI SfcI SfoI SgfI SmaI SmlI SpeI
SphI StyI TaqII TatI TstI Tth111I XbaI XcmI
XmaI
Enzymes that do not cut:
AarI AatII AfeI AflII AhdI AleI AlfI AloI
ApaI AscI AvrII BaeI BbvCI BcgI BglI Bpu10I
BsiWI BsmBI BssHII BstEII BstXI BstZ17I Bsu36I BtgI
ClaI DraIII EcoNI FseI FspAI HpaI NcoI NotI
NruI PacI PmlI PpuMI PspOMI PspXI SacII SanDI
SexAI SfiI SgrAI SnaBI SrfI SspI StuI SwaI
XhoI XmnI ZraI