Restriction map for
pF1KE0091
(Circular) (Six-base) MAPSORT of: pF1KE0091.seq Check: 4329 from: 1 to: 5009
pF1KE0091 5009 bp
With 182 enzymes: SgfI *
April 24, 2009 13:10 ..
AccI GT'mk_AC
Cuts at: 853 869 2047 853
Size: 16 1178 3815
Fragments arranged by size:
3815 1178 16
Acc65I G'GTAC_C
Cuts at: 1200 2025 1200
Size: 825 4184
AclI AA'CG_TT
Cuts at: 4849 4849
Size: 5009
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 2834 3266 4013 4281 2834
Size: 432 747 268 3562
Fragments arranged by size:
3562 747 432 268
AflIII A'CryG_T
Cuts at: 323 466 1379 2361 3465 4442 323
Size: 143 913 982 1104 977 890
Fragments arranged by size:
1104 982 977 913 890 143
AgeI A'CCGG_T
Cuts at: 1589 3397 1589
Size: 1808 3201
AlwNI CAG_nnn'CTG
Cuts at: 663 1106 2314 3881 663
Size: 443 1208 1567 1791
Fragments arranged by size:
1791 1567 1208 443
ApaLI G'TGCA_C
Cuts at: 3779 3779
Size: 5009
ApoI r'AATT_y
Cuts at: 407 2013 4362 4618 407
Size: 1606 2349 256 798
Fragments arranged by size:
2349 1606 798 256
AseI AT'TA_AT
Cuts at: 20 20
Size: 5009
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 5009
AvaI C'yCGr_G
Cuts at: 934 1849 2029 934
Size: 915 180 3914
Fragments arranged by size:
3914 915 180
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'
Cuts at: 816 849 816
Size: 33 4976
BamHI G'GATC_C
Cuts at: 2034 2034
Size: 5009
BanI G'GyrC_C
Cuts at: 174 207 1135 1200 2025 2439 2687 2722 174
Size: 33 928 65 825 414 248 35 2461
Fragments arranged by size:
2461 928 825 414 248 65 35 33
BanII G_rGCy'C
Cuts at: 1918 2023 3053 1918
Size: 105 1030 3874
Fragments arranged by size:
3874 1030 105
BbeI G_GCGC'C
Cuts at: 178 211 2443 2691 178
Size: 33 2232 248 2496
Fragments arranged by size:
2496 2232 248 33
BbsI GAAGACnn'nnnn_
Cuts at: 1616 1837 1616
Size: 221 4788
BbvCI CC'TCA_GC
Cuts at: 152 1211 152
Size: 1059 3950
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1125 1159 1548 1582 1125
Size: 34 389 34 4552
Fragments arranged by size:
4552 389 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 2907 3668 5009 2907
Size: 761 1341 2907
Fragments arranged by size:
2907 1341 761
BclI T'GATC_A
Cuts at: 1658 1703 2529 1658
Size: 45 826 4138
Fragments arranged by size:
4138 826 45
BglI GCCn_nnn'nGGC
Cuts at: 112 112
Size: 5009
BglII A'GATC_T
Cuts at: 1022 1884 2524 4561 1022
Size: 862 640 2037 1470
Fragments arranged by size:
2037 1470 862 640
BlpI GC'TnA_GC
Cuts at: 289 2121 289
Size: 1832 3177
Bme1580I G_kGCm'C
Cuts at: 2634 2727 3783 2634
Size: 93 1056 3860
Fragments arranged by size:
3860 1056 93
BmrI ACTGGGnnnn_n'
Cuts at: 2221 2302 2637 4781 2221
Size: 81 335 2144 2449
Fragments arranged by size:
2449 2144 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3376 3376
Size: 5009
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 445 1843 445
Size: 1398 3611
Bpu10I CC'TnA_GC
Cuts at: 152 580 1211 152
Size: 428 631 3950
Fragments arranged by size:
3950 631 428
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1705 1939 2185 3064 3556 3854 1705
Size: 234 246 879 492 298 2860
Fragments arranged by size:
2860 879 492 298 246 234
BsaI GGTCTCn'nnnn_
Cuts at: 4713 4713
Size: 5009
BsaAI yAC'GTr
Cuts at: 384 467 873 2362 384
Size: 83 406 1489 3031
Fragments arranged by size:
3031 1489 406 83
BsaBI GATnn'nnATC
Cuts at: 6 1315 1690 1816 2523 4560 6
Size: 1309 375 126 707 2037 455
Fragments arranged by size:
2037 1309 707 455 375 126
BsaHI Gr'CG_yC
Cuts at: 175 208 2440 2688 3390 4886 175
Size: 33 2232 248 702 1496 298
Fragments arranged by size:
2232 1496 702 298 248 33
BsaWI w'CCGG_w
Cuts at: 1397 1589 2198 2719 3397 3671 3818 4229 1397
Size: 192 609 521 678 274 147 411 2177
Fragments arranged by size:
2177 678 609 521 411 274 192 147
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 169 374 169
Size: 205 4804
BseYI C'CCAG_C
Cuts at: 315 782 1150 1571 1746 1876 2434 3192 3769
Size: 467 368 421 175 130 558 758 577
Cuts at: 3769 4265 315
Size: 496 1059
Fragments arranged by size:
1059 758 577 558 496 467 421 368
175 130
BsiEI CG_ry'CG
Cuts at: 104 2551 2597 3805 104
Size: 2447 46 1208 1308
Fragments arranged by size:
2447 1308 1208 46
BsiHKAI G_wGCw'C
Cuts at: 734 767 2023 2801 2991 3783 734
Size: 33 1256 778 190 792 1960
Fragments arranged by size:
1960 1256 792 778 190 33
BsiWI C'GTAC_G
Cuts at: 1187 1187
Size: 5009
BsmI GAATG_Cn'
Cuts at: 367 705 367
Size: 338 4671
BsmBI CGTCTCn'nnnn_
Cuts at: 1987 1987
Size: 5009
Bsp1286I G_dGCh'C
Cuts at: 734 767 1918 2023 2634 2727 2801 2991 3053
Size: 33 1151 105 611 93 74 190 62
Cuts at: 3053 3783 734
Size: 730 1960
Fragments arranged by size:
1960 1151 730 611 190 105 93 74
62 33
BspEI T'CCGG_A
Cuts at: 1397 4229 1397
Size: 2832 2177
BspHI T'CATG_A
Cuts at: 1307 1661 4185 1307
Size: 354 2524 2131
Fragments arranged by size:
2524 2131 354
BspMI ACCTGCnnnn'nnnn_
Cuts at: 2059 2273 2575 2956 2059
Size: 214 302 381 4112
Fragments arranged by size:
4112 381 302 214
BsrBI CCG'CTC
Cuts at: 50 1063 1806 3301 3355 4839 5004 50
Size: 1013 743 1495 54 1484 165 55
Fragments arranged by size:
1495 1484 1013 743 165 55 54
BsrDI GCAATG_nn'
Cuts at: 144 1205 2921 144
Size: 1061 1716 2232
Fragments arranged by size:
2232 1716 1061
BsrFI r'CCGG_y
Cuts at: 1409 1589 1625 3007 3188 3397 1409
Size: 180 36 1382 181 209 3021
Fragments arranged by size:
3021 1382 209 181 180 36
BsrGI T'GTAC_A
Cuts at: 1487 1487
Size: 5009
BssHII G'CGCG_C
Cuts at: 214 214
Size: 5009
BssSI C'ACGA_G
Cuts at: 250 1462 3280 3638 250
Size: 1212 1818 358 1621
Fragments arranged by size:
1818 1621 1212 358
BstAPI GCAn_nnn'nTGC
Cuts at: 2386 2386
Size: 5009
BstBI TT'CG_AA
Cuts at: 3370 3370
Size: 5009
BstEII G'GTnAC_C
Cuts at: 1263 1362 1405 1263
Size: 99 43 4867
Fragments arranged by size:
4867 99 43
BstXI CCAn_nnnn'nTGG
Cuts at: 1047 1146 1047
Size: 99 4910
BstYI r'GATC_y
Cuts at: 1 1022 1673 1685 1811 1884 2034 2501 2524
Size: 1021 651 12 126 73 150 467 23
Cuts at: 2524 2859 3105 4117 4203 4215 4561 1
Size: 335 246 1012 86 12 346 449
Fragments arranged by size:
1021 1012 651 467 449 346 335 246
150 126 86 73 23 12 12
BstZ17I GTA'TAC
Cuts at: 870 870
Size: 5009
BtgI C'CryG_G
Cuts at: 1370 1918 1370
Size: 548 4461
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 1956 2959 3140 3303 1956
Size: 1003 181 163 3662
Fragments arranged by size:
3662 1003 181 163
BtsI GCAGTG_nn'
Cuts at: 1843 2252 1843
Size: 409 4600
DraI TTT'AAA
Cuts at: 2008 2008
Size: 5009
DraIII CAC_nnn'GTG
Cuts at: 1826 1826
Size: 5009
DrdI GACnn_nn'nnGTC
Cuts at: 244 997 2715 3573 244
Size: 753 1718 858 1680
Fragments arranged by size:
1718 1680 858 753
EaeI y'GGCC_r
Cuts at: 258 296 743 1544 1627 2594 2768 3159 3186
Size: 38 447 801 83 967 174 391 27
Cuts at: 3186 4531 258
Size: 1345 736
Fragments arranged by size:
1345 967 801 736 447 391 174 83
38 27
EagI C'GGCC_G
Cuts at: 2594 2594
Size: 5009
EarI CTCTTCn'nnn_
Cuts at: 1543 3032 3242 1543
Size: 1489 210 3310
Fragments arranged by size:
3310 1489 210
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 130 3217 3527 3673 4819 130
Size: 3087 310 146 1146 320
Fragments arranged by size:
3087 1146 320 310 146
EcoICRI GAG'CTC
Cuts at: 2021 2021
Size: 5009
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 445 1843 2834 3266 4013 4281 445
Size: 1398 991 432 747 268 1173
Fragments arranged by size:
1398 1173 991 747 432 268
EcoO109I rG'GnC_Cy
Cuts at: 738 938 1228 2148 738
Size: 200 290 920 3599
Fragments arranged by size:
3599 920 290 200
EcoRI G'AATT_C
Cuts at: 407 2013 407
Size: 1606 3403
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 978 3028 978
Size: 2050 2959
FspI TGC'GCA
Cuts at: 2790 2790
Size: 5009
HaeII r_GCGC'y
Cuts at: 127 178 211 333 2443 2691 3713 4692 127
Size: 51 33 122 2110 248 1022 979 444
Fragments arranged by size:
2110 1022 979 444 248 122 51 33
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 2851 2881 2883 2913 2851
Size: 30 2 30 4947
Fragments arranged by size:
4947 30 30 2
HincII GTy'rAC
Cuts at: 1472 1868 2048 1472
Size: 396 180 4433
Fragments arranged by size:
4433 396 180
HindIII A'AGCT_T
Cuts at: 1903 1903
Size: 5009
HpaI GTT'AAC
Cuts at: 1472 1472
Size: 5009
KasI G'GCGC_C
Cuts at: 174 207 2439 2687 174
Size: 33 2232 248 2496
Fragments arranged by size:
2496 2232 248 33
KpnI G_GTAC'C
Cuts at: 1204 2029 1204
Size: 825 4184
MluI A'CGCG_T
Cuts at: 4442 4442
Size: 5009
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 451 1013 3680 3864 451
Size: 562 2667 184 1596
Fragments arranged by size:
2667 1596 562 184
MscI TGG'CCA
Cuts at: 745 1546 2770 745
Size: 801 1224 2984
Fragments arranged by size:
2984 1224 801
MslI CAynn'nnrTG
Cuts at: 536 1270 1822 2998 3125 536
Size: 734 552 1176 127 2420
Fragments arranged by size:
2420 1176 734 552 127
MspA1I CmG'CkG
Cuts at: 197 1133 1287 2120 2434 2794 3807 4052 197
Size: 936 154 833 314 360 1013 245 1154
Fragments arranged by size:
1154 1013 936 833 360 314 245 154
NaeI GCC'GGC
Cuts at: 3190 3190
Size: 5009
NarI GG'CG_CC
Cuts at: 175 208 2440 2688 175
Size: 33 2232 248 2496
Fragments arranged by size:
2496 2232 248 33
NgoMIV G'CCGG_C
Cuts at: 3188 3188
Size: 5009
NspI r_CATG'y
Cuts at: 1383 1488 2062 2562 3469 1383
Size: 105 574 500 907 2923
Fragments arranged by size:
2923 907 574 500 105
PasI CC'CwG_GG
Cuts at: 132 608 1669 132
Size: 476 1061 3472
Fragments arranged by size:
3472 1061 476
PciI A'CATG_T
Cuts at: 1379 3465 1379
Size: 2086 2923
PfoI T'CCnGG_A
Cuts at: 422 422
Size: 5009
PmeI GTTT'AAAC
Cuts at: 2008 2008
Size: 5009
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4148 4180 4148
Size: 32 4977
PpuMI rG'GwC_Cy
Cuts at: 738 738
Size: 5009
PsiI TTA'TAA
Cuts at: 1076 1085 1076
Size: 9 5000
PstI C_TGCA'G
Cuts at: 193 698 2056 2741 193
Size: 505 1358 685 2461
Fragments arranged by size:
2461 1358 685 505
PvuI CG_AT'CG
Cuts at: 104 104
Size: 5009
PvuII CAG'CTG
Cuts at: 2434 2794 2434
Size: 360 4649
RsrII CG'GwC_CG
Cuts at: 3204 3204
Size: 5009
SacI G_AGCT'C
Cuts at: 2023 2023
Size: 5009
SalI G'TCGA_C
Cuts at: 2046 2046
Size: 5009
SapI GCTCTTCn'nnn_
Cuts at: 3032 3242 3032
Size: 210 4799
SbfI CC_TGCA'GG
Cuts at: 2056 2056
Size: 5009
ScaI AGT'ACT
Cuts at: 4526 4526
Size: 5009
SfcI C'TryA_G
Cuts at: 33 189 678 694 854 1098 2052 2737 3730
Size: 156 489 16 160 244 954 685 993
Cuts at: 3730 3921 33
Size: 191 1121
Fragments arranged by size:
1121 993 954 685 489 244 191 160
156 16
SfoI GGC'GCC
Cuts at: 176 209 2441 2689 176
Size: 33 2232 248 2496
Fragments arranged by size:
2496 2232 248 33
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 5009
SmaI CCC'GGG
Cuts at: 936 2031 936
Size: 1095 3914
SmlI C'TyrA_G
Cuts at: 1720 1954 2164 3079 3571 3833 1720
Size: 234 210 915 492 262 2896
Fragments arranged by size:
2896 915 492 262 234 210
SpeI A'CTAG_T
Cuts at: 67 67
Size: 5009
SphI G_CATG'C
Cuts at: 2062 2562 2062
Size: 500 4509
StyI C'CwwG_G
Cuts at: 1256 1388 2143 2497 1256
Size: 132 755 354 3768
Fragments arranged by size:
3768 755 354 132
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 1563 2592 2725 3184 3393 1563
Size: 1029 133 459 209 3179
Fragments arranged by size:
3179 1029 459 209 133
TatI w'GTAC_w
Cuts at: 542 1487 1709 1724 4524 542
Size: 945 222 15 2800 1027
Fragments arranged by size:
2800 1027 945 222 15
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 4526 4558 4526
Size: 32 4977
Tth111I GACn'n_nGTC
Cuts at: 849 2806 849
Size: 1957 3052
XbaI T'CTAG_A
Cuts at: 2040 2040
Size: 5009
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 1212 2504 1212
Size: 1292 3717
XmaI C'CCGG_G
Cuts at: 934 2029 934
Size: 1095 3914
Enzymes that do cut:
AccI Acc65I AclI AcuI AflIII AgeI AlwNI ApaLI
ApoI AseI AsiSI AvaI BaeI BamHI BanI BanII
BbeI BbsI BbvCI BcgI BciVI BclI BglI BglII
BlpI Bme1580I BmrI BplI BpmI Bpu10I BpuEI BsaI
BsaAI BsaBI BsaHI BsaWI BseRI BseYI BsiEI BsiHKAI
BsiWI BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI
BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII
BstXI BstYI BstZ17I BtgI BtgZI BtsI DraI DraIII
DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoO109I
EcoRI FalI FspI HaeII Hin4I HincII HindIII HpaI
KasI KpnI MluI MmeI MscI MslI MspA1I NaeI
NarI NgoMIV NspI PasI PciI PfoI PmeI PpiI
PpuMI PsiI PstI PvuI PvuII RsrII SacI SalI
SapI SbfI ScaI SfcI SfoI SgfI SmaI SmlI
SpeI SphI StyI TaqII TatI TstI Tth111I XbaI
XcmI XmaI
Enzymes that do not cut:
AarI AatII AfeI AflII AhdI AleI AlfI AloI
ApaI AscI AvrII BfrBI BmgBI BmtI BsaXI BsgI
Bsu36I ClaI CspCI EcoNI EcoRV FseI FspAI MfeI
NcoI NdeI NheI NotI NruI NsiI PacI PflMI
PmlI PshAI PspOMI PspXI PsrI SacII SanDI SexAI
SfiI SgrAI SnaBI SrfI SspI StuI SwaI XhoI
XmnI ZraI