Restriction map for
pF1KE0092
(Circular) (Six-base) MAPSORT of: pF1KE0092.seq Check: 7893 from: 1 to: 8828
pF1KE0092 8828 bp
With 182 enzymes: SgfI *
April 24, 2009 13:10 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 168 5277 168
Size: 5109 3719
AatII G_ACGT'C
Cuts at: 3477 3477
Size: 8828
AccI GT'mk_AC
Cuts at: 2067 5866 2067
Size: 3799 5029
Acc65I G'GTAC_C
Cuts at: 565 4907 5594 5844 565
Size: 4342 687 250 3549
Fragments arranged by size:
4342 3549 687 250
AclI AA'CG_TT
Cuts at: 8668 8668
Size: 8828
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 290 764 868 1143 1295 1556 1920 1926 2431
Size: 474 104 275 152 261 364 6 505
Cuts at: 2431 2839 3209 3754 3802 3808 3814 5759 6653
Size: 408 370 545 48 6 6 1945 894
Cuts at: 6653 7085 7832 8100 290
Size: 432 747 268 1018
Fragments arranged by size:
1945 1018 894 747 545 505 474 432
408 370 364 275 268 261 152 104
48 6 6 6
AflII C'TTAA_G
Cuts at: 3721 3721
Size: 8828
AflIII A'CryG_T
Cuts at: 4723 5140 6180 7284 8261 4723
Size: 417 1040 1104 977 5290
Fragments arranged by size:
5290 1104 1040 977 417
AgeI A'CCGG_T
Cuts at: 7216 7216
Size: 8828
AhdI GACnn_n'nnGTC
Cuts at: 2916 2916
Size: 8828
AleI CACnn'nnGTG
Cuts at: 5495 5495
Size: 8828
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 3038 3433 4607 3038
Size: 395 1174 7259
Fragments arranged by size:
7259 1174 395
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 3219 3251 3219
Size: 32 8796
AlwNI CAG_nnn'CTG
Cuts at: 1345 1615 2610 4036 4135 6133 7700 1345
Size: 270 995 1426 99 1998 1567 2473
Fragments arranged by size:
2473 1998 1567 1426 995 270 99
ApaI G_GGCC'C
Cuts at: 3672 3672
Size: 8828
ApaLI G'TGCA_C
Cuts at: 7598 7598
Size: 8828
ApoI r'AATT_y
Cuts at: 630 5299 5832 8181 8437 630
Size: 4669 533 2349 256 1021
Fragments arranged by size:
4669 2349 1021 533 256
AseI AT'TA_AT
Cuts at: 20 20
Size: 8828
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 8828
AvaI C'yCGr_G
Cuts at: 456 3665 3671 3697 4478 5050 5848 456
Size: 3209 6 26 781 572 798 3436
Fragments arranged by size:
3436 3209 798 781 572 26 6
AvrII C'CTAG_G
Cuts at: 1227 3712 1227
Size: 2485 6343
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'
Cuts at: 4891 4924 4891
Size: 33 8795
BamHI G'GATC_C
Cuts at: 5853 5853
Size: 8828
BanI G'GyrC_C
Cuts at: 565 1023 4827 4907 4978 4990 5039 5057 5594
Size: 458 3804 80 71 12 49 18 537
Cuts at: 5594 5844 6258 6506 6541 565
Size: 250 414 248 35 2852
Fragments arranged by size:
3804 2852 537 458 414 250 248 80
71 49 35 18 12
BanII G_rGCy'C
Cuts at: 1010 3053 3672 4580 4922 5453 5842 6872 1010
Size: 2043 619 908 342 531 389 1030 2966
Fragments arranged by size:
2966 2043 1030 908 619 531 389 342
BbeI G_GCGC'C
Cuts at: 5043 6262 6510 5043
Size: 1219 248 7361
Fragments arranged by size:
7361 1219 248
BbsI GAAGACnn'nnnn_
Cuts at: 980 1001 1494 1759 4639 4757 980
Size: 21 493 265 2880 118 5051
Fragments arranged by size:
5051 2880 493 265 118 21
BbvCI CC'TCA_GC
Cuts at: 1527 3812 3898 4160 4557 4832 1527
Size: 2285 86 262 397 275 5523
Fragments arranged by size:
5523 2285 397 275 262 86
BciVI GTATCCnnnnn_n'
Cuts at: 754 4424 4495 6726 7487 8828 754
Size: 3670 71 2231 761 1341 754
Fragments arranged by size:
3670 2231 1341 761 754 71
BclI T'GATC_A
Cuts at: 963 1347 1886 6348 963
Size: 384 539 4462 3443
Fragments arranged by size:
4462 3443 539 384
BglI GCCn_nnn'nGGC
Cuts at: 4926 4926
Size: 8828
BglII A'GATC_T
Cuts at: 2870 6343 8380 2870
Size: 3473 2037 3318
Fragments arranged by size:
3473 3318 2037
BlpI GC'TnA_GC
Cuts at: 3782 3925 4963 5282 5940 3782
Size: 143 1038 319 658 6670
Fragments arranged by size:
6670 1038 658 319 143
Bme1580I G_kGCm'C
Cuts at: 3672 4983 4993 5759 6453 6546 7602 3672
Size: 1311 10 766 694 93 1056 4898
Fragments arranged by size:
4898 1311 1056 766 694 93 10
BmgBI CAC'GTC
Cuts at: 1680 1680
Size: 8828
BmrI ACTGGGnnnn_n'
Cuts at: 444 2642 3188 3492 3886 4334 6040 6121 6456
Size: 2198 546 304 394 448 1706 81 335
Cuts at: 6456 8600 444
Size: 2144 672
Fragments arranged by size:
2198 2144 1706 672 546 448 394 335
304 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 1720 3950 4431 4791 5751 7195 1720
Size: 2230 481 360 960 1444 3353
Fragments arranged by size:
3353 2230 1444 960 481 360
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 321 1024 1418 1442 1478 1502 1715 2215 3115
Size: 703 394 24 36 24 213 500 900
Cuts at: 3115 3871 4202 4301 4699 4969 5119 321
Size: 756 331 99 398 270 150 4030
Fragments arranged by size:
4030 900 756 703 500 398 394 331
270 213 150 99 36 24 24
Bpu10I CC'TnA_GC
Cuts at: 1527 1897 3812 3898 4160 4416 4557 4832 5650
Size: 370 1915 86 262 256 141 275 818
Cuts at: 5650 1527
Size: 4705
Fragments arranged by size:
4705 1915 818 370 275 262 256 141
86
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 412 1120 4049 4447 6004 6883 7375 7673 412
Size: 708 2929 398 1557 879 492 298 1567
Fragments arranged by size:
2929 1567 1557 879 708 492 398 298
BsaI GGTCTCn'nnnn_
Cuts at: 224 909 1518 1828 2323 3247 3453 4268 4326
Size: 685 609 310 495 924 206 815 58
Cuts at: 4326 8532 224
Size: 4206 520
Fragments arranged by size:
4206 924 815 685 609 520 495 310
206 58
BsaAI yAC'GTr
Cuts at: 6181 6181
Size: 8828
BsaBI GATnn'nnATC
Cuts at: 6 6342 8379 6
Size: 6336 2037 455
Fragments arranged by size:
6336 2037 455
BsaHI Gr'CG_yC
Cuts at: 3474 5040 5388 6259 6507 7209 8705 3474
Size: 1566 348 871 248 702 1496 3597
Fragments arranged by size:
3597 1566 1496 871 702 348 248
BsaWI w'CCGG_w
Cuts at: 3146 6017 6538 7216 7490 7637 8048 3146
Size: 2871 521 678 274 147 411 3926
Fragments arranged by size:
3926 2871 678 521 411 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1825 1855 2017 2047 2872 2902 3088 3118 3219
Size: 30 162 30 825 30 186 30 101
Cuts at: 3219 3249 5521 5551 1825
Size: 30 2272 30 5102
Fragments arranged by size:
5102 2272 825 186 162 101 30 30
30 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 324 1749 2038 2233 2479 3265 3298 3559 4039
Size: 1425 289 195 246 786 33 261 480
Cuts at: 4039 4164 4687 5689 5806 324
Size: 125 523 1002 117 3346
Fragments arranged by size:
3346 1425 1002 786 523 480 289 261
246 195 125 117 33
BseYI C'CCAG_C
Cuts at: 236 361 538 1334 1410 1539 1608 1635 2709
Size: 125 177 796 76 129 69 27 1074
Cuts at: 2709 2987 3002 3011 3764 3800 3806 4002 4246
Size: 278 15 9 753 36 6 196 244
Cuts at: 4246 4308 4379 4391 5418 5752 5786 6253 7011
Size: 62 71 12 1027 334 34 467 758
Cuts at: 7011 7588 8084 236
Size: 577 496 980
Fragments arranged by size:
1074 1027 980 796 758 753 577 496
467 334 278 244 196 177 129 125
76 71 69 62 36 34 27 15
12 9 6
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 140 1100 2029 2810 4243 140
Size: 960 929 781 1433 4725
Fragments arranged by size:
4725 1433 960 929 781
BsiEI CG_ry'CG
Cuts at: 104 6370 6416 7624 104
Size: 6266 46 1208 1308
Fragments arranged by size:
6266 1308 1208 46
BsiHKAI G_wGCw'C
Cuts at: 1934 3053 4417 4772 5842 6620 6810 7602 1934
Size: 1119 1364 355 1070 778 190 792 3160
Fragments arranged by size:
3160 1364 1119 1070 792 778 355 190
BsmI GAATG_Cn'
Cuts at: 704 3220 5027 704
Size: 2516 1807 4505
Fragments arranged by size:
4505 2516 1807
BsmBI CGTCTCn'nnnn_
Cuts at: 1731 1731
Size: 8828
Bsp1286I G_dGCh'C
Cuts at: 1010 1934 3053 3672 4417 4580 4772 4922 4983
Size: 924 1119 619 745 163 192 150 61
Cuts at: 4983 4993 5453 5759 5842 6453 6546 6620 6810
Size: 10 460 306 83 611 93 74 190
Cuts at: 6810 6872 7602 1010
Size: 62 730 2236
Fragments arranged by size:
2236 1119 924 745 730 619 611 460
306 192 190 163 150 93 83 74
62 61 10
BspEI T'CCGG_A
Cuts at: 3146 8048 3146
Size: 4902 3926
BspHI T'CATG_A
Cuts at: 1730 4935 8004 1730
Size: 3205 3069 2554
Fragments arranged by size:
3205 3069 2554
BspMI ACCTGCnnnn'nnnn_
Cuts at: 168 2810 4599 4919 5277 5878 6092 6394 6775
Size: 2642 1789 320 358 601 214 302 381
Cuts at: 6775 168
Size: 2221
Fragments arranged by size:
2642 2221 1789 601 381 358 320 302
214
BsrBI CCG'CTC
Cuts at: 50 5055 5643 7120 7174 8658 8823 50
Size: 5005 588 1477 54 1484 165 55
Fragments arranged by size:
5005 1484 1477 588 165 55 54
BsrDI GCAATG_nn'
Cuts at: 2641 4110 5108 6740 2641
Size: 1469 998 1632 4729
Fragments arranged by size:
4729 1632 1469 998
BsrFI r'CCGG_y
Cuts at: 497 3140 5597 6826 7007 7216 497
Size: 2643 2457 1229 181 209 2109
Fragments arranged by size:
2643 2457 2109 1229 209 181
BssSI C'ACGA_G
Cuts at: 2624 7099 7457 2624
Size: 4475 358 3995
Fragments arranged by size:
4475 3995 358
BstAPI GCAn_nnn'nTGC
Cuts at: 163 2007 2236 2802 3589 5763 6205 163
Size: 1844 229 566 787 2174 442 2786
Fragments arranged by size:
2786 2174 1844 787 566 442 229
BstBI TT'CG_AA
Cuts at: 7189 7189
Size: 8828
BstEII G'GTnAC_C
Cuts at: 2469 2469
Size: 8828
BstXI CCAn_nnnn'nTGG
Cuts at: 114 2839 3264 4242 4308 4854 114
Size: 2725 425 978 66 546 4088
Fragments arranged by size:
4088 2725 978 546 425 66
BstYI r'GATC_y
Cuts at: 1 697 2027 2870 2894 2924 4437 5853 6320
Size: 696 1330 843 24 30 1513 1416 467
Cuts at: 6320 6343 6678 6924 7936 8022 8034 8380 1
Size: 23 335 246 1012 86 12 346 449
Fragments arranged by size:
1513 1416 1330 1012 843 696 467 449
346 335 246 86 30 24 23 12
Bsu36I CC'TnA_GG
Cuts at: 168 192 2138 3542 3776 4985 168
Size: 24 1946 1404 234 1209 4011
Fragments arranged by size:
4011 1946 1404 1209 234 24
BtgI C'CryG_G
Cuts at: 107 578 5202 107
Size: 471 4624 3733
Fragments arranged by size:
4624 3733 471
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 6778 6959 7122 6778
Size: 181 163 8484
Fragments arranged by size:
8484 181 163
BtsI GCAGTG_nn'
Cuts at: 744 1670 1706 2008 3647 4661 6071 744
Size: 926 36 302 1639 1014 1410 3501
Fragments arranged by size:
3501 1639 1410 1014 926 302 36
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 1765 1800 1765
Size: 35 8793
DraI TTT'AAA
Cuts at: 5827 5827
Size: 8828
DrdI GACnn_nn'nnGTC
Cuts at: 2768 2806 6534 7392 2768
Size: 38 3728 858 4204
Fragments arranged by size:
4204 3728 858 38
EaeI y'GGCC_r
Cuts at: 2131 2510 3167 3306 4927 5331 5379 6413 6587
Size: 379 657 139 1621 404 48 1034 174
Cuts at: 6587 6978 7005 8350 2131
Size: 391 27 1345 2609
Fragments arranged by size:
2609 1621 1345 1034 657 404 391 379
174 139 48 27
EagI C'GGCC_G
Cuts at: 6413 6413
Size: 8828
EarI CTCTTCn'nnn_
Cuts at: 827 1679 1855 3266 3275 3315 3616 4236 6851
Size: 852 176 1411 9 40 301 620 2615
Cuts at: 6851 7061 827
Size: 210 2594
Fragments arranged by size:
2615 2594 1411 852 620 301 210 176
40 9
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 1803 1912 7036 7346 7492 8638 1803
Size: 109 5124 310 146 1146 1993
Fragments arranged by size:
5124 1993 1146 310 146 109
EcoICRI GAG'CTC
Cuts at: 3051 5840 3051
Size: 2789 6039
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 290 321 764 868 1024 1143 1295 1418 1442
Size: 31 443 104 156 119 152 123 24
Cuts at: 1442 1478 1502 1556 1715 1920 1926 2215 2431
Size: 36 24 54 159 205 6 289 216
Cuts at: 2431 2839 3115 3209 3754 3802 3808 3814 3871
Size: 408 276 94 545 48 6 6 57
Cuts at: 3871 4202 4301 4699 4969 5119 5759 6653 7085
Size: 331 99 398 270 150 640 894 432
Cuts at: 7085 7832 8100 290
Size: 747 268 1018
Fragments arranged by size:
1018 894 747 640 545 443 432 408
398 331 289 276 270 268 216 205
159 156 152 150 123 119 104 99
94 57 54 48 36 31 24 24
6 6 6
EcoNI CCTnn'n_nnAGG
Cuts at: 191 1226 2755 3457 191
Size: 1035 1529 702 5562
Fragments arranged by size:
5562 1529 1035 702
EcoO109I rG'GnC_Cy
Cuts at: 201 432 2748 4467 4956 5428 5967 201
Size: 231 2316 1719 489 472 539 3062
Fragments arranged by size:
3062 2316 1719 539 489 472 231
EcoRI G'AATT_C
Cuts at: 5299 5832 5299
Size: 533 8295
EcoRV GAT'ATC
Cuts at: 4794 5006 4794
Size: 212 8616
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 1783 6847 1783
Size: 5064 3764
FspI TGC'GCA
Cuts at: 1801 2983 6609 1801
Size: 1182 3626 4020
Fragments arranged by size:
4020 3626 1182
FspAI rTGC'GCAy
Cuts at: 1801 1801
Size: 8828
HaeII r_GCGC'y
Cuts at: 2526 3372 5043 6262 6510 7532 8511 2526
Size: 846 1671 1219 248 1022 979 2843
Fragments arranged by size:
2843 1671 1219 1022 979 846 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1339 1371 2017 2049 2872 2902 2904 2934 3088
Size: 32 646 32 823 30 2 30 154
Cuts at: 3088 3120 4033 4065 4422 4454 5007 5039 5521
Size: 32 913 32 357 32 553 32 482
Cuts at: 5521 5553 6670 6700 6702 6732 1339
Size: 32 1117 30 2 30 3435
Fragments arranged by size:
3435 1117 913 823 646 553 482 357
154 32 32 32 32 32 32 32
30 30 30 30 2 2
HincII GTy'rAC
Cuts at: 1565 2068 5130 5867 1565
Size: 503 3062 737 4526
Fragments arranged by size:
4526 3062 737 503
KasI G'GCGC_C
Cuts at: 5039 6258 6506 5039
Size: 1219 248 7361
Fragments arranged by size:
7361 1219 248
KpnI G_GTAC'C
Cuts at: 569 4911 5598 5848 569
Size: 4342 687 250 3549
Fragments arranged by size:
4342 3549 687 250
MluI A'CGCG_T
Cuts at: 8261 8261
Size: 8828
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1648 1800 1941 3665 4430 4802 7499 7683 1648
Size: 152 141 1724 765 372 2697 184 2793
Fragments arranged by size:
2793 2697 1724 765 372 184 152 141
MscI TGG'CCA
Cuts at: 2512 3169 4929 5333 6589 2512
Size: 657 1760 404 1256 4751
Fragments arranged by size:
4751 1760 1256 657 404
MslI CAynn'nnrTG
Cuts at: 112 752 5495 6817 6944 112
Size: 640 4743 1322 127 1996
Fragments arranged by size:
4743 1996 1322 640 127
MspA1I CmG'CkG
Cuts at: 1615 3298 4383 4561 4686 5097 5786 5939 6253
Size: 1683 1085 178 125 411 689 153 314
Cuts at: 6253 6613 7626 7871 1615
Size: 360 1013 245 2572
Fragments arranged by size:
2572 1683 1085 1013 689 411 360 314
245 178 153 125
NaeI GCC'GGC
Cuts at: 499 3142 7009 499
Size: 2643 3867 2318
Fragments arranged by size:
3867 2643 2318
NarI GG'CG_CC
Cuts at: 5040 6259 6507 5040
Size: 1219 248 7361
Fragments arranged by size:
7361 1219 248
NcoI C'CATG_G
Cuts at: 107 578 107
Size: 471 8357
NdeI CA'TA_TG
Cuts at: 2949 5010 2949
Size: 2061 6767
NgoMIV G'CCGG_C
Cuts at: 497 3140 7007 497
Size: 2643 3867 2318
Fragments arranged by size:
3867 2643 2318
NspI r_CATG'y
Cuts at: 4727 5144 5881 6381 7288 4727
Size: 417 737 500 907 6267
Fragments arranged by size:
6267 907 737 500 417
PasI CC'CwG_GG
Cuts at: 3453 3453
Size: 8828
PciI A'CATG_T
Cuts at: 4723 5140 7284 4723
Size: 417 2144 6267
Fragments arranged by size:
6267 2144 417
PflMI CCAn_nnn'nTGG
Cuts at: 1039 5114 5329 1039
Size: 4075 215 4538
Fragments arranged by size:
4538 4075 215
PfoI T'CCnGG_A
Cuts at: 3848 3987 4487 3848
Size: 139 500 8189
Fragments arranged by size:
8189 500 139
PmeI GTTT'AAAC
Cuts at: 5827 5827
Size: 8828
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3219 3251 7967 7999 3219
Size: 32 4716 32 4048
Fragments arranged by size:
4716 4048 32 32
PpuMI rG'GwC_Cy
Cuts at: 432 2748 432
Size: 2316 6512
PspOMI G'GGCC_C
Cuts at: 3668 3668
Size: 8828
PspXI vC'TCGA_Gb
Cuts at: 5050 5050
Size: 8828
PstI C_TGCA'G
Cuts at: 2756 4131 4709 5875 6560 2756
Size: 1375 578 1166 685 5024
Fragments arranged by size:
5024 1375 1166 685 578
PvuI CG_AT'CG
Cuts at: 104 104
Size: 8828
PvuII CAG'CTG
Cuts at: 1615 3298 4383 4561 5097 6253 6613 1615
Size: 1683 1085 178 536 1156 360 3830
Fragments arranged by size:
3830 1683 1156 1085 536 360 178
RsrII CG'GwC_CG
Cuts at: 7023 7023
Size: 8828
SacI G_AGCT'C
Cuts at: 3053 5842 3053
Size: 2789 6039
SalI G'TCGA_C
Cuts at: 2066 5865 2066
Size: 3799 5029
SapI GCTCTTCn'nnn_
Cuts at: 827 6851 7061 827
Size: 6024 210 2594
Fragments arranged by size:
6024 2594 210
SbfI CC_TGCA'GG
Cuts at: 2756 5875 2756
Size: 3119 5709
ScaI AGT'ACT
Cuts at: 8345 8345
Size: 8828
SexAI A'CCwGG_T
Cuts at: 1837 1837
Size: 8828
SfcI C'TryA_G
Cuts at: 33 1481 2752 3111 3290 3468 4127 4705 4997
Size: 1448 1271 359 179 178 659 578 292
Cuts at: 4997 5871 6556 7549 7740 33
Size: 874 685 993 191 1121
Fragments arranged by size:
1448 1271 1121 993 874 685 659 578
359 292 191 179 178
SfoI GGC'GCC
Cuts at: 5041 6260 6508 5041
Size: 1219 248 7361
Fragments arranged by size:
7361 1219 248
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 8828
SmaI CCC'GGG
Cuts at: 3667 3699 5850 3667
Size: 32 2151 6645
Fragments arranged by size:
6645 2151 32
SmlI C'TyrA_G
Cuts at: 427 1099 3721 4064 4462 5050 5983 6898 7390
Size: 672 2622 343 398 588 933 915 492
Cuts at: 7390 7652 427
Size: 262 1603
Fragments arranged by size:
2622 1603 933 915 672 588 492 398
343 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 8828
SphI G_CATG'C
Cuts at: 5881 6381 5881
Size: 500 8328
SrfI GCCC'GGGC
Cuts at: 3667 3667
Size: 8828
StyI C'CwwG_G
Cuts at: 107 486 578 1227 2108 2298 2369 3712 4241
Size: 379 92 649 881 190 71 1343 529
Cuts at: 4241 5962 6316 107
Size: 1721 354 2619
Fragments arranged by size:
2619 1721 1343 881 649 529 379 354
190 92 71
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 1066 1772 1995 2776 3001 5183 6411 6544 7003
Size: 706 223 781 225 2182 1228 133 459
Cuts at: 7003 7212 1066
Size: 209 2682
Fragments arranged by size:
2682 2182 1228 781 706 459 225 223
209 133
TatI w'GTAC_w
Cuts at: 5266 8343 5266
Size: 3077 5751
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 8345 8377 8345
Size: 32 8796
Tth111I GACn'n_nGTC
Cuts at: 6625 6625
Size: 8828
XbaI T'CTAG_A
Cuts at: 1662 5859 1662
Size: 4197 4631
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 1677 5255 6323 1677
Size: 3578 1068 4182
Fragments arranged by size:
4182 3578 1068
XhoI C'TCGA_G
Cuts at: 5050 5050
Size: 8828
XmaI C'CCGG_G
Cuts at: 3665 3697 5848 3665
Size: 32 2151 6645
Fragments arranged by size:
6645 2151 32
XmnI GAAnn'nnTTC
Cuts at: 1760 1760
Size: 8828
ZraI GAC'GTC
Cuts at: 3475 3475
Size: 8828
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AflII AflIII
AgeI AhdI AleI AlfI AloI AlwNI ApaI ApaLI
ApoI AseI AsiSI AvaI AvrII BaeI BamHI BanI
BanII BbeI BbsI BbvCI BciVI BclI BglI BglII
BlpI Bme1580I BmgBI BmrI BplI BpmI Bpu10I BpuEI
BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI
BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI
BspMI BsrBI BsrDI BsrFI BssSI BstAPI BstBI BstEII
BstXI BstYI Bsu36I BtgI BtgZI BtsI CspCI DraI
DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI
EcoO109I EcoRI EcoRV FalI FspI FspAI HaeII Hin4I
HincII KasI KpnI MluI MmeI MscI MslI MspA1I
NaeI NarI NcoI NdeI NgoMIV NspI PasI PciI
PflMI PfoI PmeI PpiI PpuMI PspOMI PspXI PstI
PvuI PvuII RsrII SacI SalI SapI SbfI ScaI
SexAI SfcI SfoI SgfI SmaI SmlI SpeI SphI
SrfI StyI TaqII TatI TstI Tth111I XbaI XcmI
XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AfeI AscI BcgI BfrBI BmtI BsiWI BsrGI BssHII
BstZ17I ClaI DraIII FseI HindIII HpaI MfeI NheI
NotI NruI NsiI PacI PmlI PshAI PsiI PsrI
SacII SanDI SfiI SgrAI SnaBI SspI StuI SwaI