Restriction map for
pF1KE0093
(Circular) (Six-base) MAPSORT of: pF1KE0093.seq Check: 4010 from: 1 to: 6398
pF1KE0093 6398 bp
With 182 enzymes: SgfI *
April 24, 2009 13:10 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 263 368 576 1453 263
Size: 105 208 877 5208
Fragments arranged by size:
5208 877 208 105
AccI GT'mk_AC
Cuts at: 899 3052 3064 3436 899
Size: 2153 12 372 3861
Fragments arranged by size:
3861 2153 372 12
Acc65I G'GTAC_C
Cuts at: 3323 3414 3323
Size: 91 6307
AclI AA'CG_TT
Cuts at: 6238 6238
Size: 6398
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 373 1785 2208 4223 4655 5402 5670 373
Size: 1412 423 2015 432 747 268 1101
Fragments arranged by size:
2015 1412 1101 747 432 423 268
AfeI AGC'GCT
Cuts at: 3331 3331
Size: 6398
AflIII A'CryG_T
Cuts at: 1780 2796 3750 4854 5831 1780
Size: 1016 954 1104 977 2347
Fragments arranged by size:
2347 1104 1016 977 954
AgeI A'CCGG_T
Cuts at: 4786 4786
Size: 6398
AhdI GACnn_n'nnGTC
Cuts at: 2871 2871
Size: 6398
AleI CACnn'nnGTG
Cuts at: 1004 1517 1004
Size: 513 5885
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1465 1535 1465
Size: 70 6328
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2494 2526 2494
Size: 32 6366
AlwNI CAG_nnn'CTG
Cuts at: 536 924 1445 1542 2282 2531 2633 3703 5270
Size: 388 521 97 740 249 102 1070 1567
Cuts at: 5270 536
Size: 1664
Fragments arranged by size:
1664 1567 1070 740 521 388 249 102
97
ApaI G_GGCC'C
Cuts at: 284 622 2087 2830 284
Size: 338 1465 743 3852
Fragments arranged by size:
3852 1465 743 338
ApaLI G'TGCA_C
Cuts at: 250 2107 2949 5168 250
Size: 1857 842 2219 1480
Fragments arranged by size:
2219 1857 1480 842
ApoI r'AATT_y
Cuts at: 2432 3402 5751 6007 2432
Size: 970 2349 256 2823
Fragments arranged by size:
2823 2349 970 256
AseI AT'TA_AT
Cuts at: 20 20
Size: 6398
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 6398
AvaI C'yCGr_G
Cuts at: 177 277 419 489 1854 2026 2234 2256 2823
Size: 100 142 70 1365 172 208 22 567
Cuts at: 2823 3089 3418 177
Size: 266 329 3157
Fragments arranged by size:
3157 1365 567 329 266 208 172 142
100 70 22
BamHI G'GATC_C
Cuts at: 3423 3423
Size: 6398
BanI G'GyrC_C
Cuts at: 118 455 541 998 1604 1660 1685 1756 1858
Size: 337 86 457 606 56 25 71 102
Cuts at: 1858 3122 3323 3414 3828 4076 4111 118
Size: 1264 201 91 414 248 35 2405
Fragments arranged by size:
2405 1264 606 457 414 337 248 201
102 91 86 71 56 35 25
BanII G_rGCy'C
Cuts at: 284 622 1750 1797 1957 2087 2297 2660 2722
Size: 338 1128 47 160 130 210 363 62
Cuts at: 2722 2830 3412 4442 284
Size: 108 582 1030 2240
Fragments arranged by size:
2240 1128 1030 582 363 338 210 160
130 108 62 47
BbeI G_GCGC'C
Cuts at: 122 459 545 1760 3126 3832 4080 122
Size: 337 86 1215 1366 706 248 2440
Fragments arranged by size:
2440 1366 1215 706 337 248 86
BbsI GAAGACnn'nnnn_
Cuts at: 1244 1244
Size: 6398
BbvCI CC'TCA_GC
Cuts at: 683 683
Size: 6398
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 171 205 2559 2593 2781 2815 171
Size: 34 2354 34 188 34 3754
Fragments arranged by size:
3754 2354 188 34 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 4296 5057 6398 4296
Size: 761 1341 4296
Fragments arranged by size:
4296 1341 761
BclI T'GATC_A
Cuts at: 644 3918 644
Size: 3274 3124
BglI GCCn_nnn'nGGC
Cuts at: 288 512 560 1754 3001 288
Size: 224 48 1194 1247 3685
Fragments arranged by size:
3685 1247 1194 224 48
BglII A'GATC_T
Cuts at: 2360 3913 5950 2360
Size: 1553 2037 2808
Fragments arranged by size:
2808 2037 1553
BlpI GC'TnA_GC
Cuts at: 529 1951 3510 529
Size: 1422 1559 3417
Fragments arranged by size:
3417 1559 1422
Bme1580I G_kGCm'C
Cuts at: 184 254 284 622 1001 2087 2111 2830 2953
Size: 70 30 338 379 1086 24 719 123
Cuts at: 2953 3142 4023 4116 5172 184
Size: 189 881 93 1056 1410
Fragments arranged by size:
1410 1086 1056 881 719 379 338 189
123 93 70 30 24
BmgBI CAC'GTC
Cuts at: 210 249 740 2799 210
Size: 39 491 2059 3809
Fragments arranged by size:
3809 2059 491 39
BmrI ACTGGGnnnn_n'
Cuts at: 3050 3610 3691 4026 6170 3050
Size: 560 81 335 2144 3278
Fragments arranged by size:
3278 2144 560 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2379 3033 4765 2379
Size: 654 1732 4012
Fragments arranged by size:
4012 1732 654
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1039 1764 1782 1859 1039
Size: 725 18 77 5578
Fragments arranged by size:
5578 725 77 18
Bpu10I CC'TnA_GC
Cuts at: 683 698 683
Size: 15 6383
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1411 2624 3574 4453 4945 5243 1411
Size: 1213 950 879 492 298 2566
Fragments arranged by size:
2566 1213 950 879 492 298
BsaI GGTCTCn'nnnn_
Cuts at: 687 805 1252 3254 6102 687
Size: 118 447 2002 2848 983
Fragments arranged by size:
2848 2002 983 447 118
BsaAI yAC'GTr
Cuts at: 3056 3751 3056
Size: 695 5703
BsaBI GATnn'nnATC
Cuts at: 6 3912 5949 6
Size: 3906 2037 455
Fragments arranged by size:
3906 2037 455
BsaHI Gr'CG_yC
Cuts at: 119 297 456 542 830 1714 1757 2887 3123
Size: 178 159 86 288 884 43 1130 236
Cuts at: 3123 3829 4077 4779 6275 119
Size: 706 248 702 1496 242
Fragments arranged by size:
1496 1130 884 706 702 288 248 242
236 178 159 86 43
BsaWI w'CCGG_w
Cuts at: 3587 4108 4786 5060 5207 5618 3587
Size: 521 678 274 147 411 4367
Fragments arranged by size:
4367 678 521 411 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 499 529 1343 1373 499
Size: 30 814 30 5524
Fragments arranged by size:
5524 814 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 451 1060 1070 3013 451
Size: 609 10 1943 3836
Fragments arranged by size:
3836 1943 609 10
BseYI C'CCAG_C
Cuts at: 312 670 1278 1912 2531 3823 4581 5158 5654
Size: 358 608 634 619 1292 758 577 496
Cuts at: 5654 312
Size: 1056
Fragments arranged by size:
1292 1056 758 634 619 608 577 496
358
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 340 862 1227 1791 2377 2562 2569 2866 3118
Size: 522 365 564 586 185 7 297 252
Cuts at: 3118 340
Size: 3620
Fragments arranged by size:
3620 586 564 522 365 297 252 185
7
BsiEI CG_ry'CG
Cuts at: 104 206 465 1947 2836 3940 3986 5194 104
Size: 102 259 1482 889 1104 46 1208 1308
Fragments arranged by size:
1482 1308 1208 1104 889 259 102 46
BsiHKAI G_wGCw'C
Cuts at: 224 254 503 803 2111 2297 2908 2953 3061
Size: 30 249 300 1308 186 611 45 108
Cuts at: 3061 3412 4190 4380 5172 224
Size: 351 778 190 792 1450
Fragments arranged by size:
1450 1308 792 778 611 351 300 249
190 186 108 45 30
BsmI GAATG_Cn'
Cuts at: 1560 1560
Size: 6398
BsmBI CGTCTCn'nnnn_
Cuts at: 528 2586 528
Size: 2058 4340
Bsp1286I G_dGCh'C
Cuts at: 184 224 254 284 503 622 803 1001 1750
Size: 40 30 30 219 119 181 198 749
Cuts at: 1750 1797 1957 2087 2111 2297 2660 2722 2830
Size: 47 160 130 24 186 363 62 108
Cuts at: 2830 2908 2953 3061 3142 3412 4023 4116 4190
Size: 78 45 108 81 270 611 93 74
Cuts at: 4190 4380 4442 5172 184
Size: 190 62 730 1410
Fragments arranged by size:
1410 749 730 611 363 270 219 198
190 186 181 160 130 119 108 108
93 81 78 74 62 62 47 45
40 30 30 24
BspEI T'CCGG_A
Cuts at: 5618 5618
Size: 6398
BspHI T'CATG_A
Cuts at: 887 5574 887
Size: 4687 1711
BspMI ACCTGCnnnn'nnnn_
Cuts at: 263 368 413 576 1322 1453 1898 2209 2776
Size: 105 45 163 746 131 445 311 567
Cuts at: 2776 3076 3352 3448 3662 3964 4345 263
Size: 300 276 96 214 302 381 2316
Fragments arranged by size:
2316 746 567 445 381 311 302 300
276 214 163 131 105 96 45
BsrBI CCG'CTC
Cuts at: 50 135 771 1011 4690 4744 6228 6393 50
Size: 85 636 240 3679 54 1484 165 55
Fragments arranged by size:
3679 1484 636 240 165 85 55 54
BsrDI GCAATG_nn'
Cuts at: 4310 4310
Size: 6398
BsrFI r'CCGG_y
Cuts at: 363 547 1946 2835 4396 4577 4786 363
Size: 184 1399 889 1561 181 209 1975
Fragments arranged by size:
1975 1561 1399 889 209 184 181
BsrGI T'GTAC_A
Cuts at: 1152 2587 1152
Size: 1435 4963
BssHII G'CGCG_C
Cuts at: 324 342 324
Size: 18 6380
BssSI C'ACGA_G
Cuts at: 2583 2982 3257 4669 5027 2583
Size: 399 275 1412 358 3954
Fragments arranged by size:
3954 1412 399 358 275
BstAPI GCAn_nnn'nTGC
Cuts at: 1500 1850 2201 3105 3137 3775 1500
Size: 350 351 904 32 638 4123
Fragments arranged by size:
4123 904 638 351 350 32
BstBI TT'CG_AA
Cuts at: 4759 4759
Size: 6398
BstEII G'GTnAC_C
Cuts at: 2763 2763
Size: 6398
BstXI CCAn_nnnn'nTGG
Cuts at: 2428 2616 2428
Size: 188 6210
BstYI r'GATC_y
Cuts at: 1 1538 2360 3423 3890 3913 4248 4494 5506
Size: 1537 822 1063 467 23 335 246 1012
Cuts at: 5506 5592 5604 5950 1
Size: 86 12 346 449
Fragments arranged by size:
1537 1063 1012 822 467 449 346 335
246 86 23 12
BstZ17I GTA'TAC
Cuts at: 900 900
Size: 6398
BtgI C'CryG_G
Cuts at: 226 459 849 1002 1750 1982 2713 2942 3126
Size: 233 390 153 748 232 731 229 184
Cuts at: 3126 226
Size: 3498
Fragments arranged by size:
3498 748 731 390 233 232 229 184
153
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 1251 4348 4529 4692 1251
Size: 3097 181 163 2957
Fragments arranged by size:
3097 2957 181 163
BtsI GCAGTG_nn'
Cuts at: 1215 1537 1848 1936 2041 2465 2550 2557 2849
Size: 322 311 88 105 424 85 7 292
Cuts at: 2849 3106 3641 1215
Size: 257 535 3972
Fragments arranged by size:
3972 535 424 322 311 292 257 105
88 85 7
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 1303 1338 1303
Size: 35 6363
DraI TTT'AAA
Cuts at: 3397 3397
Size: 6398
DraIII CAC_nnn'GTG
Cuts at: 807 1667 2211 807
Size: 860 544 4994
Fragments arranged by size:
4994 860 544
DrdI GACnn_nn'nnGTC
Cuts at: 4104 4962 4104
Size: 858 5540
EaeI y'GGCC_r
Cuts at: 203 462 504 824 2051 2833 3983 4157 4548
Size: 259 42 320 1227 782 1150 174 391
Cuts at: 4548 4575 5920 203
Size: 27 1345 681
Fragments arranged by size:
1345 1227 1150 782 681 391 320 259
174 42 27
EagI C'GGCC_G
Cuts at: 203 462 2833 3983 203
Size: 259 2371 1150 2618
Fragments arranged by size:
2618 2371 1150 259
EarI CTCTTCn'nnn_
Cuts at: 4421 4631 4421
Size: 210 6188
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 2573 4606 4916 5062 6208 2573
Size: 2033 310 146 1146 2763
Fragments arranged by size:
2763 2033 1146 310 146
EcoICRI GAG'CTC
Cuts at: 2295 3410 2295
Size: 1115 5283
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 373 1039 1764 1782 1785 1859 2208 4223 4655
Size: 666 725 18 3 74 349 2015 432
Cuts at: 4655 5402 5670 373
Size: 747 268 1101
Fragments arranged by size:
2015 1101 747 725 666 432 349 268
74 18 3
EcoNI CCTnn'n_nnAGG
Cuts at: 2029 3307 2029
Size: 1278 5120
EcoO109I rG'GnC_Cy
Cuts at: 281 618 1334 2061 2084 2827 3078 3537 281
Size: 337 716 727 23 743 251 459 3142
Fragments arranged by size:
3142 743 727 716 459 337 251 23
EcoRI G'AATT_C
Cuts at: 2432 3402 2432
Size: 970 5428
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2238 4417 2238
Size: 2179 4219
FspI TGC'GCA
Cuts at: 4179 4179
Size: 6398
HaeII r_GCGC'y
Cuts at: 122 459 536 545 1760 3126 3333 3832 4080
Size: 337 77 9 1215 1366 207 499 248
Cuts at: 4080 5102 6081 122
Size: 1022 979 439
Fragments arranged by size:
1366 1215 1022 979 499 439 337 248
207 77 9
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1014 1046 1300 1303 1332 1335 2162 2194 2598
Size: 32 254 3 29 3 827 32 404
Cuts at: 2598 2630 2857 2889 4240 4270 4272 4302 1014
Size: 32 227 32 1351 30 2 30 3110
Fragments arranged by size:
3110 1351 827 404 254 227 32 32
32 32 30 30 29 3 3 2
HincII GTy'rAC
Cuts at: 1992 2601 3065 3437 1992
Size: 609 464 372 4953
Fragments arranged by size:
4953 609 464 372
HindIII A'AGCT_T
Cuts at: 955 955
Size: 6398
KasI G'GCGC_C
Cuts at: 118 455 541 1756 3122 3828 4076 118
Size: 337 86 1215 1366 706 248 2440
Fragments arranged by size:
2440 1366 1215 706 337 248 86
KpnI G_GTAC'C
Cuts at: 3327 3418 3327
Size: 91 6307
MluI A'CGCG_T
Cuts at: 5831 5831
Size: 6398
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1161 5069 5253 1161
Size: 3908 184 2306
Fragments arranged by size:
3908 2306 184
MscI TGG'CCA
Cuts at: 4159 4159
Size: 6398
MslI CAynn'nnrTG
Cuts at: 892 946 1004 1160 1307 1517 4387 4514 892
Size: 54 58 156 147 210 2870 127 2776
Fragments arranged by size:
2870 2776 210 156 147 127 58 54
MspA1I CmG'CkG
Cuts at: 187 264 393 461 2186 2391 2528 2781 2915
Size: 77 129 68 1725 205 137 253 134
Cuts at: 2915 3509 3823 4183 5196 5441 187
Size: 594 314 360 1013 245 1144
Fragments arranged by size:
1725 1144 1013 594 360 314 253 245
205 137 134 129 77 68
NaeI GCC'GGC
Cuts at: 365 549 2837 4579 365
Size: 184 2288 1742 2184
Fragments arranged by size:
2288 2184 1742 184
NarI GG'CG_CC
Cuts at: 119 456 542 1757 3123 3829 4077 119
Size: 337 86 1215 1366 706 248 2440
Fragments arranged by size:
2440 1366 1215 706 337 248 86
NcoI C'CATG_G
Cuts at: 1002 1002
Size: 6398
NgoMIV G'CCGG_C
Cuts at: 363 547 2835 4577 363
Size: 184 2288 1742 2184
Fragments arranged by size:
2288 2184 1742 184
NotI GC'GGCC_GC
Cuts at: 462 462
Size: 6398
NspI r_CATG'y
Cuts at: 1512 1732 1770 1784 1876 3108 3318 3451 3951
Size: 220 38 14 92 1232 210 133 500
Cuts at: 3951 4858 1512
Size: 907 3052
Fragments arranged by size:
3052 1232 907 500 220 210 133 92
38 14
PasI CC'CwG_GG
Cuts at: 615 1831 615
Size: 1216 5182
PciI A'CATG_T
Cuts at: 1780 4854 1780
Size: 3074 3324
PflMI CCAn_nnn'nTGG
Cuts at: 628 924 1278 1290 628
Size: 296 354 12 5736
Fragments arranged by size:
5736 354 296 12
PfoI T'CCnGG_A
Cuts at: 1721 1919 2063 1721
Size: 198 144 6056
Fragments arranged by size:
6056 198 144
PmeI GTTT'AAAC
Cuts at: 3397 3397
Size: 6398
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 5537 5569 5537
Size: 32 6366
PpuMI rG'GwC_Cy
Cuts at: 1334 2061 1334
Size: 727 5671
PshAI GACnn'nnGTC
Cuts at: 1244 1244
Size: 6398
PspOMI G'GGCC_C
Cuts at: 280 618 2083 2826 280
Size: 338 1465 743 3852
Fragments arranged by size:
3852 1465 743 338
PsrI GAACnnnnnnTACnnnnnnn_nnnnn'
Cuts at: 883 915 883
Size: 32 6366
PstI C_TGCA'G
Cuts at: 1402 1570 2157 2185 2786 3086 3349 3445 4130
Size: 168 587 28 601 300 263 96 685
Cuts at: 4130 1402
Size: 3670
Fragments arranged by size:
3670 685 601 587 300 263 168 96
28
PvuI CG_AT'CG
Cuts at: 104 104
Size: 6398
PvuII CAG'CTG
Cuts at: 187 393 2781 2915 3823 4183 187
Size: 206 2388 134 908 360 2402
Fragments arranged by size:
2402 2388 908 360 206 134
RsrII CG'GwC_CG
Cuts at: 2939 4593 2939
Size: 1654 4744
SacI G_AGCT'C
Cuts at: 2297 3412 2297
Size: 1115 5283
SacII CC_GC'GG
Cuts at: 462 462
Size: 6398
SalI G'TCGA_C
Cuts at: 3063 3435 3063
Size: 372 6026
SapI GCTCTTCn'nnn_
Cuts at: 4421 4631 4421
Size: 210 6188
SbfI CC_TGCA'GG
Cuts at: 3349 3445 3349
Size: 96 6302
ScaI AGT'ACT
Cuts at: 5915 5915
Size: 6398
SexAI A'CCwGG_T
Cuts at: 1079 1079
Size: 6398
SfcI C'TryA_G
Cuts at: 33 1398 1515 1566 2153 2181 2782 3082 3165
Size: 1365 117 51 587 28 601 300 83
Cuts at: 3165 3345 3441 4126 5119 5310 33
Size: 180 96 685 993 191 1121
Fragments arranged by size:
1365 1121 993 685 601 587 300 191
180 117 96 83 51 28
SfiI GGCCn_nnn'nGGCC
Cuts at: 560 560
Size: 6398
SfoI GGC'GCC
Cuts at: 120 457 543 1758 3124 3830 4078 120
Size: 337 86 1215 1366 706 248 2440
Fragments arranged by size:
2440 1366 1215 706 337 248 86
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 6398
SgrAI Cr'CCGG_yG
Cuts at: 547 547
Size: 6398
SmaI CCC'GGG
Cuts at: 179 279 1856 2236 2825 3091 3420 179
Size: 100 1577 380 589 266 329 3157
Fragments arranged by size:
3157 1577 589 380 329 266 100
SmlI C'TyrA_G
Cuts at: 1426 2639 3553 4468 4960 5222 1426
Size: 1213 914 915 492 262 2602
Fragments arranged by size:
2602 1213 915 914 492 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 6398
SphI G_CATG'C
Cuts at: 1512 3108 3451 3951 1512
Size: 1596 343 500 3959
Fragments arranged by size:
3959 1596 500 343
SrfI GCCC'GGGC
Cuts at: 2825 2825
Size: 6398
StuI AGG'CCT
Cuts at: 750 1970 3004 750
Size: 1220 1034 4144
Fragments arranged by size:
4144 1220 1034
StyI C'CwwG_G
Cuts at: 1002 1901 2369 3532 3886 1002
Size: 899 468 1163 354 3514
Fragments arranged by size:
3514 1163 899 468 354
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 1347 2691 3981 4114 4573 4782 1347
Size: 1344 1290 133 459 209 2963
Fragments arranged by size:
2963 1344 1290 459 209 133
TatI w'GTAC_w
Cuts at: 1152 2587 5913 1152
Size: 1435 3326 1637
Fragments arranged by size:
3326 1637 1435
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 499 531 1153 1185 1343 1362 1375 1394 1915
Size: 32 622 32 158 19 13 19 521
Cuts at: 1915 1947 3304 3336 5915 5947 499
Size: 32 1357 32 2579 32 950
Fragments arranged by size:
2579 1357 950 622 521 158 32 32
32 32 32 19 19 13
Tth111I GACn'n_nGTC
Cuts at: 974 2059 4195 974
Size: 1085 2136 3177
Fragments arranged by size:
3177 2136 1085
XbaI T'CTAG_A
Cuts at: 3429 3429
Size: 6398
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 1543 3893 1543
Size: 2350 4048
XmaI C'CCGG_G
Cuts at: 177 277 1854 2234 2823 3089 3418 177
Size: 100 1577 380 589 266 329 3157
Fragments arranged by size:
3157 1577 589 380 329 266 100
XmnI GAAnn'nnTTC
Cuts at: 2746 2746
Size: 6398
Enzymes that do cut:
AarI AccI Acc65I AclI AcuI AfeI AflIII AgeI
AhdI AleI AlfI AloI AlwNI ApaI ApaLI ApoI
AseI AsiSI AvaI BamHI BanI BanII BbeI BbsI
BbvCI BcgI BciVI BclI BglI BglII BlpI Bme1580I
BmgBI BmrI BplI BpmI Bpu10I BpuEI BsaI BsaAI
BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI
BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI
BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII
BstXI BstYI BstZ17I BtgI BtgZI BtsI CspCI DraI
DraIII DrdI EaeI EagI EarI EciI EcoICRI Eco57MI
EcoNI EcoO109I EcoRI FalI FspI HaeII Hin4I HincII
HindIII KasI KpnI MluI MmeI MscI MslI MspA1I
NaeI NarI NcoI NgoMIV NotI NspI PasI PciI
PflMI PfoI PmeI PpiI PpuMI PshAI PspOMI PsrI
PstI PvuI PvuII RsrII SacI SacII SalI SapI
SbfI ScaI SexAI SfcI SfiI SfoI SgfI SgrAI
SmaI SmlI SpeI SphI SrfI StuI StyI TaqII
TatI TstI Tth111I XbaI XcmI XmaI XmnI
Enzymes that do not cut:
AatII AflII AscI AvrII BaeI BfrBI BmtI BsiWI
Bsu36I ClaI EcoRV FseI FspAI HpaI MfeI NdeI
NheI NruI NsiI PacI PmlI PsiI PspXI SanDI
SnaBI SspI SwaI XhoI ZraI