Restriction map for
pF1KE0095
(Circular) (Six-base) MAPSORT of: pF1KE0095.seq Check: 2252 from: 1 to: 6896
pF1KE0095 6896 bp
With 182 enzymes: SgfI *
April 24, 2009 13:10 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 3117 3117
Size: 6896
AatII G_ACGT'C
Cuts at: 1859 1859
Size: 6896
AccI GT'mk_AC
Cuts at: 2378 3934 2378
Size: 1556 5340
Acc65I G'GTAC_C
Cuts at: 3912 3912
Size: 6896
AclI AA'CG_TT
Cuts at: 6736 6736
Size: 6896
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 375 1255 1709 2322 4721 5153 5900 6168 375
Size: 880 454 613 2399 432 747 268 1103
Fragments arranged by size:
2399 1103 880 747 613 454 432 268
AfeI AGC'GCT
Cuts at: 802 802
Size: 6896
AflIII A'CryG_T
Cuts at: 2663 2738 3776 3800 4248 5352 6329 2663
Size: 75 1038 24 448 1104 977 3230
Fragments arranged by size:
3230 1104 1038 977 448 75 24
AgeI A'CCGG_T
Cuts at: 3742 5284 3742
Size: 1542 5354
AhdI GACnn_n'nnGTC
Cuts at: 1860 1860
Size: 6896
AleI CACnn'nnGTG
Cuts at: 1715 1823 2159 2452 2638 1715
Size: 108 336 293 186 5973
Fragments arranged by size:
5973 336 293 186 108
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 173 173
Size: 6896
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 1459 1491 1459
Size: 32 6864
AlwNI CAG_nnn'CTG
Cuts at: 312 323 1230 1575 2807 3789 4201 5768 312
Size: 11 907 345 1232 982 412 1567 1440
Fragments arranged by size:
1567 1440 1232 982 907 412 345 11
ApaI G_GGCC'C
Cuts at: 704 3881 704
Size: 3177 3719
ApaLI G'TGCA_C
Cuts at: 1560 5666 1560
Size: 4106 2790
ApoI r'AATT_y
Cuts at: 1799 3900 6249 6505 1799
Size: 2101 2349 256 2190
Fragments arranged by size:
2349 2190 2101 256
AseI AT'TA_AT
Cuts at: 20 20
Size: 6896
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 6896
AvaI C'yCGr_G
Cuts at: 135 173 244 277 491 684 690 1340 1677
Size: 38 71 33 214 193 6 650 337
Cuts at: 1677 2827 3916 135
Size: 1150 1089 3115
Fragments arranged by size:
3115 1150 1089 650 337 214 193 71
38 33 6
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'
Cuts at: 3731 3764 3731
Size: 33 6863
BamHI G'GATC_C
Cuts at: 3921 3921
Size: 6896
BanI G'GyrC_C
Cuts at: 162 168 495 634 724 2392 2952 3912 4326
Size: 6 327 139 90 1668 560 960 414
Cuts at: 4326 4574 4609 162
Size: 248 35 2449
Fragments arranged by size:
2449 1668 960 560 414 327 248 139
90 35 6
BanII G_rGCy'C
Cuts at: 133 253 691 704 1598 2930 3293 3644 3881
Size: 120 438 13 894 1332 363 351 237
Cuts at: 3881 3910 4940 133
Size: 29 1030 2089
Fragments arranged by size:
2089 1332 1030 894 438 363 351 237
120 29 13
BbeI G_GCGC'C
Cuts at: 166 172 499 638 728 4330 4578 166
Size: 6 327 139 90 3602 248 2484
Fragments arranged by size:
3602 2484 327 248 139 90 6
BbsI GAAGACnn'nnnn_
Cuts at: 160 2210 2412 160
Size: 2050 202 4644
Fragments arranged by size:
4644 2050 202
BbvCI CC'TCA_GC
Cuts at: 1307 1307
Size: 6896
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1260 1294 1802 1836 1260
Size: 34 508 34 6320
Fragments arranged by size:
6320 508 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 4794 5555 6896 4794
Size: 761 1341 4794
Fragments arranged by size:
4794 1341 761
BclI T'GATC_A
Cuts at: 4416 4416
Size: 6896
BglI GCCn_nnn'nGGC
Cuts at: 137 928 2401 137
Size: 791 1473 4632
Fragments arranged by size:
4632 1473 791
BglII A'GATC_T
Cuts at: 4411 6448 4411
Size: 2037 4859
BlpI GC'TnA_GC
Cuts at: 355 1162 2135 2476 2854 4008 355
Size: 807 973 341 378 1154 3243
Fragments arranged by size:
3243 1154 973 807 378 341
Bme1580I G_kGCm'C
Cuts at: 704 1564 2114 2459 3040 3681 3881 4521 4614
Size: 860 550 345 581 641 200 640 93
Cuts at: 4614 5670 704
Size: 1056 1930
Fragments arranged by size:
1930 1056 860 641 640 581 550 345
200 93
BmgBI CAC'GTC
Cuts at: 2316 3249 2316
Size: 933 5963
BmrI ACTGGGnnnn_n'
Cuts at: 1551 2136 3027 4108 4189 4524 6668 1551
Size: 585 891 1081 81 335 2144 1779
Fragments arranged by size:
2144 1779 1081 891 585 335 81
BmtI G_CTAG'C
Cuts at: 1882 1882
Size: 6896
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 1767 5263 1767
Size: 3496 3400
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 607 646 805 889 2562 2664 3388 3484 3832
Size: 39 159 84 1673 102 724 96 348
Cuts at: 3832 607
Size: 3671
Fragments arranged by size:
3671 1673 724 348 159 102 96 84
39
Bpu10I CC'TnA_GC
Cuts at: 1307 3041 1307
Size: 1734 5162
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1987 4072 4951 5443 5741 1987
Size: 2085 879 492 298 3142
Fragments arranged by size:
3142 2085 879 492 298
BsaI GGTCTCn'nnnn_
Cuts at: 1317 1330 1840 2014 6600 1317
Size: 13 510 174 4586 1613
Fragments arranged by size:
4586 1613 510 174 13
BsaAI yAC'GTr
Cuts at: 1642 2672 2748 2937 3803 4249 1642
Size: 1030 76 189 866 446 4289
Fragments arranged by size:
4289 1030 866 446 189 76
BsaBI GATnn'nnATC
Cuts at: 6 952 1489 2449 3634 4410 6447 6
Size: 946 537 960 1185 776 2037 455
Fragments arranged by size:
2037 1185 960 946 776 537 455
BsaHI Gr'CG_yC
Cuts at: 163 169 496 635 725 1552 1856 2282 3076
Size: 6 327 139 90 827 304 426 794
Cuts at: 3076 4327 4575 5277 6773 163
Size: 1251 248 702 1496 286
Fragments arranged by size:
1496 1251 827 794 702 426 327 304
286 248 139 90 6
BsaWI w'CCGG_w
Cuts at: 3742 4085 4606 5284 5558 5705 6116 3742
Size: 343 521 678 274 147 411 4522
Fragments arranged by size:
4522 678 521 411 343 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 778 808 3013 3043 778
Size: 30 2205 30 4631
Fragments arranged by size:
4631 2205 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 604 3193 3202 3211 604
Size: 2589 9 9 4289
Fragments arranged by size:
4289 2589 9 9
BseYI C'CCAG_C
Cuts at: 2691 3122 4321 5079 5656 6152 2691
Size: 431 1199 758 577 496 3435
Fragments arranged by size:
3435 1199 758 577 496 431
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1254 1275 1550 2025 3100 3211 1254
Size: 21 275 475 1075 111 4939
Fragments arranged by size:
4939 1075 475 275 111 21
BsiEI CG_ry'CG
Cuts at: 104 444 617 935 2385 2762 3756 4438 4484
Size: 340 173 318 1450 377 994 682 46
Cuts at: 4484 5692 104
Size: 1208 1308
Fragments arranged by size:
1450 1308 1208 994 682 377 340 318
173 46
BsiHKAI G_wGCw'C
Cuts at: 860 1163 1175 1564 1598 2012 2315 2714 2912
Size: 303 12 389 34 414 303 399 198
Cuts at: 2912 2930 2942 3293 3611 3910 4688 4878 5670
Size: 18 12 351 318 299 778 190 792
Cuts at: 5670 860
Size: 2086
Fragments arranged by size:
2086 792 778 414 399 389 351 318
303 303 299 198 190 34 18 12
12
BsmBI CGTCTCn'nnnn_
Cuts at: 1680 1680
Size: 6896
Bsp1286I G_dGCh'C
Cuts at: 133 253 691 704 860 1163 1175 1564 1598
Size: 120 438 13 156 303 12 389 34
Cuts at: 1598 2012 2114 2315 2459 2714 2912 2930 2942
Size: 414 102 201 144 255 198 18 12
Cuts at: 2942 3040 3293 3611 3644 3681 3881 3910 4521
Size: 98 253 318 33 37 200 29 611
Cuts at: 4521 4614 4688 4878 4940 5670 133
Size: 93 74 190 62 730 1359
Fragments arranged by size:
1359 730 611 438 414 389 318 303
255 253 201 200 198 190 156 144
120 102 98 93 74 62 37 34
33 29 18 13 12 12
BspEI T'CCGG_A
Cuts at: 6116 6116
Size: 6896
BspHI T'CATG_A
Cuts at: 1580 6072 1580
Size: 4492 2404
BspMI ACCTGCnnnn'nnnn_
Cuts at: 2364 2696 2993 3117 3122 3946 4160 4462 4843
Size: 332 297 124 5 824 214 302 381
Cuts at: 4843 2364
Size: 4417
Fragments arranged by size:
4417 824 381 332 302 297 214 124
5
BsrBI CCG'CTC
Cuts at: 50 194 359 401 594 758 789 903 3256
Size: 144 165 42 193 164 31 114 2353
Cuts at: 3256 3276 3885 5188 5242 6726 6891 50
Size: 20 609 1303 54 1484 165 55
Fragments arranged by size:
2353 1484 1303 609 193 165 165 164
144 114 55 54 42 31 20
BsrDI GCAATG_nn'
Cuts at: 3288 4808 3288
Size: 1520 5376
BsrFI r'CCGG_y
Cuts at: 165 455 631 3215 3742 4894 5075 5284 165
Size: 290 176 2584 527 1152 181 209 1777
Fragments arranged by size:
2584 1777 1152 527 290 209 181 176
BssHII G'CGCG_C
Cuts at: 609 609
Size: 6896
BssSI C'ACGA_G
Cuts at: 859 5167 5525 859
Size: 4308 358 2230
Fragments arranged by size:
4308 2230 358
BstAPI GCAn_nnn'nTGC
Cuts at: 4273 4273
Size: 6896
BstBI TT'CG_AA
Cuts at: 5257 5257
Size: 6896
BstEII G'GTnAC_C
Cuts at: 288 2991 3765 288
Size: 2703 774 3419
Fragments arranged by size:
3419 2703 774
BstXI CCAn_nnnn'nTGG
Cuts at: 843 1281 1755 1890 2126 2161 2637 3249 3732
Size: 438 474 135 236 35 476 612 483
Cuts at: 3732 843
Size: 4007
Fragments arranged by size:
4007 612 483 476 474 438 236 135
35
BstYI r'GATC_y
Cuts at: 1 1649 3921 4388 4411 4746 4992 6004 6090
Size: 1648 2272 467 23 335 246 1012 86
Cuts at: 6090 6102 6448 1
Size: 12 346 449
Fragments arranged by size:
2272 1648 1012 467 449 346 335 246
86 23 12
Bsu36I CC'TnA_GG
Cuts at: 2243 2243
Size: 6896
BtgI C'CryG_G
Cuts at: 258 1028 1430 3656 258
Size: 770 402 2226 3498
Fragments arranged by size:
3498 2226 770 402
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 1008 1182 2795 3321 3402 4846 5027 5190 1008
Size: 174 1613 526 81 1444 181 163 2714
Fragments arranged by size:
2714 1613 1444 526 181 174 163 81
BtsI GCAGTG_nn'
Cuts at: 1163 1562 1710 1818 2563 3137 4139 1163
Size: 399 148 108 745 574 1002 3920
Fragments arranged by size:
3920 1002 745 574 399 148 108
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 3038 3073 3038
Size: 35 6861
DraI TTT'AAA
Cuts at: 3895 3895
Size: 6896
DrdI GACnn_nn'nnGTC
Cuts at: 2989 4602 5460 2989
Size: 1613 858 4425
Fragments arranged by size:
4425 1613 858
EaeI y'GGCC_r
Cuts at: 314 374 473 614 845 920 932 1037 1193
Size: 60 99 141 231 75 12 105 156
Cuts at: 1193 1619 2759 2918 3251 3560 3662 3722 4481
Size: 426 1140 159 333 309 102 60 759
Cuts at: 4481 4655 5046 5073 6418 314
Size: 174 391 27 1345 792
Fragments arranged by size:
1345 1140 792 759 426 391 333 309
231 174 159 156 141 105 102 99
75 60 60 27 12
EagI C'GGCC_G
Cuts at: 614 932 2759 4481 614
Size: 318 1827 1722 3029
Fragments arranged by size:
3029 1827 1722 318
EarI CTCTTCn'nnn_
Cuts at: 1603 2054 2431 2557 2974 4919 5129 1603
Size: 451 377 126 417 1945 210 3370
Fragments arranged by size:
3370 1945 451 417 377 210 126
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 399 694 802 1170 2767 5104 5414 5560 6706
Size: 295 108 368 1597 2337 310 146 1146
Cuts at: 6706 399
Size: 589
Fragments arranged by size:
2337 1597 1146 589 368 310 295 146
108
EcoICRI GAG'CTC
Cuts at: 1596 2928 3291 3908 1596
Size: 1332 363 617 4584
Fragments arranged by size:
4584 1332 617 363
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 375 607 646 805 889 1255 1709 2322 2562
Size: 232 39 159 84 366 454 613 240
Cuts at: 2562 2664 3388 3484 3832 4721 5153 5900 6168
Size: 102 724 96 348 889 432 747 268
Cuts at: 6168 375
Size: 1103
Fragments arranged by size:
1103 889 747 724 613 454 432 366
348 268 240 232 159 102 96 84
39
EcoNI CCTnn'n_nnAGG
Cuts at: 3241 3241
Size: 6896
EcoO109I rG'GnC_Cy
Cuts at: 181 700 2685 3026 3877 3878 4035 181
Size: 519 1985 341 851 1 157 3042
Fragments arranged by size:
3042 1985 851 519 341 157 1
EcoRI G'AATT_C
Cuts at: 1799 3900 1799
Size: 2101 4795
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 4915 4915
Size: 6896
FspI TGC'GCA
Cuts at: 1144 1207 4677 1144
Size: 63 3470 3363
Fragments arranged by size:
3470 3363 63
FspAI rTGC'GCAy
Cuts at: 1144 1144
Size: 6896
HaeII r_GCGC'y
Cuts at: 166 172 312 499 638 728 804 1082 2142
Size: 6 140 187 139 90 76 278 1060
Cuts at: 2142 4330 4578 5600 6579 166
Size: 2188 248 1022 979 483
Fragments arranged by size:
2188 1060 1022 979 483 278 248 187
140 139 90 76 6
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 673 705 1334 1366 1846 1878 4738 4768 4770
Size: 32 629 32 480 32 2860 30 2
Cuts at: 4770 4800 673
Size: 30 2769
Fragments arranged by size:
2860 2769 629 480 32 32 32 30
30 2
HincII GTy'rAC
Cuts at: 2661 2736 2766 3935 2661
Size: 75 30 1169 5622
Fragments arranged by size:
5622 1169 75 30
KasI G'GCGC_C
Cuts at: 162 168 495 634 724 4326 4574 162
Size: 6 327 139 90 3602 248 2484
Fragments arranged by size:
3602 2484 327 248 139 90 6
KpnI G_GTAC'C
Cuts at: 3916 3916
Size: 6896
MluI A'CGCG_T
Cuts at: 2738 6329 2738
Size: 3591 3305
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 755 5567 5751 755
Size: 4812 184 1900
Fragments arranged by size:
4812 1900 184
MscI TGG'CCA
Cuts at: 316 1195 2920 3724 4657 316
Size: 879 1725 804 933 2555
Fragments arranged by size:
2555 1725 933 879 804
MslI CAynn'nnrTG
Cuts at: 841 847 961 1027 1435 1715 1735 1792 1823
Size: 6 114 66 408 280 20 57 31
Cuts at: 1823 2159 2452 2470 2638 2935 3493 3775 4885
Size: 336 293 18 168 297 558 282 1110
Cuts at: 4885 5012 841
Size: 127 2725
Fragments arranged by size:
2725 1110 558 408 336 297 293 282
280 168 127 114 66 57 31 20
18 6
MspA1I CmG'CkG
Cuts at: 260 323 354 462 479 909 1166 1374 1808
Size: 63 31 108 17 430 257 208 434
Cuts at: 1808 2891 3462 3810 4007 4321 4681 5694 5939
Size: 1083 571 348 197 314 360 1013 245
Cuts at: 5939 260
Size: 1217
Fragments arranged by size:
1217 1083 1013 571 434 430 360 348
314 257 245 208 197 108 63 31
17
NaeI GCC'GGC
Cuts at: 167 457 633 5077 167
Size: 290 176 4444 1986
Fragments arranged by size:
4444 1986 290 176
NarI GG'CG_CC
Cuts at: 163 169 496 635 725 4327 4575 163
Size: 6 327 139 90 3602 248 2484
Fragments arranged by size:
3602 2484 327 248 139 90 6
NcoI C'CATG_G
Cuts at: 1430 1430
Size: 6896
NgoMIV G'CCGG_C
Cuts at: 165 455 631 5075 165
Size: 290 176 4444 1986
Fragments arranged by size:
4444 1986 290 176
NheI G'CTAG_C
Cuts at: 1878 1878
Size: 6896
NotI GC'GGCC_GC
Cuts at: 932 932
Size: 6896
NspI r_CATG'y
Cuts at: 1149 1791 2667 3049 3780 3949 4449 5356 1149
Size: 642 876 382 731 169 500 907 2689
Fragments arranged by size:
2689 907 876 731 642 500 382 169
PasI CC'CwG_GG
Cuts at: 720 720
Size: 6896
PciI A'CATG_T
Cuts at: 2663 3776 5352 2663
Size: 1113 1576 4207
Fragments arranged by size:
4207 1576 1113
PflMI CCAn_nnn'nTGG
Cuts at: 323 1050 1978 3129 3789 323
Size: 727 928 1151 660 3430
Fragments arranged by size:
3430 1151 928 727 660
PfoI T'CCnGG_A
Cuts at: 1331 1760 2578 1331
Size: 429 818 5649
Fragments arranged by size:
5649 818 429
PmeI GTTT'AAAC
Cuts at: 3895 3895
Size: 6896
PmlI CAC'GTG
Cuts at: 2672 2748 2672
Size: 76 6820
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3436 3468 6035 6067 3436
Size: 32 2567 32 4265
Fragments arranged by size:
4265 2567 32 32
PpuMI rG'GwC_Cy
Cuts at: 2685 3026 2685
Size: 341 6555
PshAI GACnn'nnGTC
Cuts at: 440 440
Size: 6896
PspOMI G'GGCC_C
Cuts at: 700 3877 700
Size: 3177 3719
PstI C_TGCA'G
Cuts at: 749 2570 2885 3170 3206 3943 4628 749
Size: 1821 315 285 36 737 685 3017
Fragments arranged by size:
3017 1821 737 685 315 285 36
PvuI CG_AT'CG
Cuts at: 104 104
Size: 6896
PvuII CAG'CTG
Cuts at: 909 2891 3462 3810 4321 4681 909
Size: 1982 571 348 511 360 3124
Fragments arranged by size:
3124 1982 571 511 360 348
RsrII CG'GwC_CG
Cuts at: 5091 5091
Size: 6896
SacI G_AGCT'C
Cuts at: 1598 2930 3293 3910 1598
Size: 1332 363 617 4584
Fragments arranged by size:
4584 1332 617 363
SacII CC_GC'GG
Cuts at: 261 261
Size: 6896
SalI G'TCGA_C
Cuts at: 3933 3933
Size: 6896
SapI GCTCTTCn'nnn_
Cuts at: 1603 2557 2974 4919 5129 1603
Size: 954 417 1945 210 3370
Fragments arranged by size:
3370 1945 954 417 210
SbfI CC_TGCA'GG
Cuts at: 3943 3943
Size: 6896
ScaI AGT'ACT
Cuts at: 2365 2833 6413 2365
Size: 468 3580 2848
Fragments arranged by size:
3580 2848 468
SexAI A'CCwGG_T
Cuts at: 2168 3782 2168
Size: 1614 5282
SfcI C'TryA_G
Cuts at: 33 745 2566 2769 2841 2881 3166 3202 3939
Size: 712 1821 203 72 40 285 36 737
Cuts at: 3939 4624 5617 5808 33
Size: 685 993 191 1121
Fragments arranged by size:
1821 1121 993 737 712 685 285 203
191 72 40 36
SfoI GGC'GCC
Cuts at: 164 170 497 636 726 4328 4576 164
Size: 6 327 139 90 3602 248 2484
Fragments arranged by size:
3602 2484 327 248 139 90 6
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 6896
SgrAI Cr'CCGG_yG
Cuts at: 165 3742 165
Size: 3577 3319
SmaI CCC'GGG
Cuts at: 137 175 246 279 493 3918 137
Size: 38 71 33 214 3425 3115
Fragments arranged by size:
3425 3115 214 71 38 33
SmlI C'TyrA_G
Cuts at: 2002 4051 4966 5458 5720 2002
Size: 2049 915 492 262 3178
Fragments arranged by size:
3178 2049 915 492 262
SnaBI TAC'GTA
Cuts at: 1642 1642
Size: 6896
SpeI A'CTAG_T
Cuts at: 67 67
Size: 6896
SphI G_CATG'C
Cuts at: 1149 3049 3949 4449 1149
Size: 1900 900 500 3596
Fragments arranged by size:
3596 1900 900 500
StuI AGG'CCT
Cuts at: 3208 3208
Size: 6896
StyI C'CwwG_G
Cuts at: 1430 1653 1830 1928 2177 2210 2510 3053 3242
Size: 223 177 98 249 33 300 543 189
Cuts at: 3242 4030 4384 1430
Size: 788 354 3942
Fragments arranged by size:
3942 788 543 354 300 249 223 189
177 98 33
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 1273 1309 2059 2149 2294 2399 4479 4612 5071
Size: 36 750 90 145 105 2080 133 459
Cuts at: 5071 5280 1273
Size: 209 2889
Fragments arranged by size:
2889 2080 750 459 209 145 133 105
90 36
TatI w'GTAC_w
Cuts at: 1815 2363 2831 3596 6411 1815
Size: 548 468 765 2815 2300
Fragments arranged by size:
2815 2300 765 548 468
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 1284 1316 6413 6445 1284
Size: 32 5097 32 1735
Fragments arranged by size:
5097 1735 32 32
Tth111I GACn'n_nGTC
Cuts at: 4693 4693
Size: 6896
XbaI T'CTAG_A
Cuts at: 3927 3927
Size: 6896
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 1894 2688 3579 4391 1894
Size: 794 891 812 4399
Fragments arranged by size:
4399 891 812 794
XmaI C'CCGG_G
Cuts at: 135 173 244 277 491 3916 135
Size: 38 71 33 214 3425 3115
Fragments arranged by size:
3425 3115 214 71 38 33
ZraI GAC'GTC
Cuts at: 1857 1857
Size: 6896
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AfeI AflIII
AgeI AhdI AleI AlfI AloI AlwNI ApaI ApaLI
ApoI AseI AsiSI AvaI BaeI BamHI BanI BanII
BbeI BbsI BbvCI BcgI BciVI BclI BglI BglII
BlpI Bme1580I BmgBI BmrI BmtI BplI BpmI Bpu10I
BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI
BseYI BsgI BsiEI BsiHKAI BsmBI Bsp1286I BspEI BspHI
BspMI BsrBI BsrDI BsrFI BssHII BssSI BstAPI BstBI
BstEII BstXI BstYI Bsu36I BtgI BtgZI BtsI CspCI
DraI DrdI EaeI EagI EarI EciI EcoICRI Eco57MI
EcoNI EcoO109I EcoRI FalI FspI FspAI HaeII Hin4I
HincII KasI KpnI MluI MmeI MscI MslI MspA1I
NaeI NarI NcoI NgoMIV NheI NotI NspI PasI
PciI PflMI PfoI PmeI PmlI PpiI PpuMI PshAI
PspOMI PstI PvuI PvuII RsrII SacI SacII SalI
SapI SbfI ScaI SexAI SfcI SfoI SgfI SgrAI
SmaI SmlI SnaBI SpeI SphI StuI StyI TaqII
TatI TstI Tth111I XbaI XcmI XmaI ZraI
Enzymes that do not cut:
AflII AscI AvrII BfrBI BsiWI BsmI BsrGI BstZ17I
ClaI DraIII EcoRV FseI HindIII HpaI MfeI NdeI
NruI NsiI PacI PsiI PspXI PsrI SanDI SfiI
SrfI SspI SwaI XhoI XmnI