Restriction map for
pF1KE0096
(Circular) (Six-base) MAPSORT of: pF1KE0096.seq Check: 6646 from: 1 to: 8618
pF1KE0096 8618 bp
With 182 enzymes: SgfI *
April 24, 2009 13:10 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 2022 3666 2022
Size: 1644 6974
AccI GT'mk_AC
Cuts at: 4017 4398 4621 5656 4017
Size: 381 223 1035 6979
Fragments arranged by size:
6979 1035 381 223
Acc65I G'GTAC_C
Cuts at: 444 2442 5634 444
Size: 1998 3192 3428
Fragments arranged by size:
3428 3192 1998
AclI AA'CG_TT
Cuts at: 8458 8458
Size: 8618
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 952 2444 2687 6443 6875 7622 7890 952
Size: 1492 243 3756 432 747 268 1680
Fragments arranged by size:
3756 1680 1492 747 432 268 243
AflIII A'CryG_T
Cuts at: 1439 3876 5970 7074 8051 1439
Size: 2437 2094 1104 977 2006
Fragments arranged by size:
2437 2094 2006 1104 977
AgeI A'CCGG_T
Cuts at: 7006 7006
Size: 8618
AhdI GACnn_n'nnGTC
Cuts at: 4617 4617
Size: 8618
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1649 2213 4340 1649
Size: 564 2127 5927
Fragments arranged by size:
5927 2127 564
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 1815 1847 1815
Size: 32 8586
AlwNI CAG_nnn'CTG
Cuts at: 2209 3297 5071 5923 7490 2209
Size: 1088 1774 852 1567 3337
Fragments arranged by size:
3337 1774 1567 1088 852
ApaLI G'TGCA_C
Cuts at: 7388 7388
Size: 8618
ApoI r'AATT_y
Cuts at: 1235 2156 2268 2379 2712 4040 4349 4476 4694
Size: 921 112 111 333 1328 309 127 218
Cuts at: 4694 5622 7971 8227 1235
Size: 928 2349 256 1626
Fragments arranged by size:
2349 1626 1328 928 921 333 309 256
218 127 112 111
AseI AT'TA_AT
Cuts at: 20 20
Size: 8618
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 8618
AvaI C'yCGr_G
Cuts at: 1363 1730 5638 1363
Size: 367 3908 4343
Fragments arranged by size:
4343 3908 367
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'
Cuts at: 2426 2459 2426
Size: 33 8585
BamHI G'GATC_C
Cuts at: 751 4957 5643 751
Size: 4206 686 3726
Fragments arranged by size:
4206 3726 686
BanI G'GyrC_C
Cuts at: 129 444 2442 5634 6048 6296 6331 129
Size: 315 1998 3192 414 248 35 2416
Fragments arranged by size:
3192 2416 1998 414 315 248 35
BanII G_rGCy'C
Cuts at: 3384 5632 6662 3384
Size: 2248 1030 5340
Fragments arranged by size:
5340 2248 1030
BbeI G_GCGC'C
Cuts at: 133 6052 6300 133
Size: 5919 248 2451
Fragments arranged by size:
5919 2451 248
BbsI GAAGACnn'nnnn_
Cuts at: 796 1036 5237 796
Size: 240 4201 4177
Fragments arranged by size:
4201 4177 240
BbvCI CC'TCA_GC
Cuts at: 4490 4490
Size: 8618
BciVI GTATCCnnnnn_n'
Cuts at: 3945 4596 4662 6516 7277 8618 3945
Size: 651 66 1854 761 1341 3945
Fragments arranged by size:
3945 1854 1341 761 651 66
BclI T'GATC_A
Cuts at: 4335 6138 4335
Size: 1803 6815
BfrBI ATG'CAT
Cuts at: 3972 3972
Size: 8618
BglII A'GATC_T
Cuts at: 6133 8170 6133
Size: 2037 6581
BlpI GC'TnA_GC
Cuts at: 1127 3458 5730 1127
Size: 2331 2272 4015
Fragments arranged by size:
4015 2331 2272
Bme1580I G_kGCm'C
Cuts at: 3539 5275 6243 6336 7392 3539
Size: 1736 968 93 1056 4765
Fragments arranged by size:
4765 1736 1056 968 93
BmgBI CAC'GTC
Cuts at: 675 675
Size: 8618
BmrI ACTGGGnnnn_n'
Cuts at: 5312 5830 5911 6246 8390 5312
Size: 518 81 335 2144 5540
Fragments arranged by size:
5540 2144 518 335 81
BmtI G_CTAG'C
Cuts at: 5509 5509
Size: 8618
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 6985 6985
Size: 8618
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1628 2186 3437 3683 4991 1628
Size: 558 1251 246 1308 5255
Fragments arranged by size:
5255 1308 1251 558 246
Bpu10I CC'TnA_GC
Cuts at: 4490 4490
Size: 8618
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2757 3752 3996 5794 6673 7165 7463 2757
Size: 995 244 1798 879 492 298 3912
Fragments arranged by size:
3912 1798 995 879 492 298 244
BsaI GGTCTCn'nnnn_
Cuts at: 259 8322 259
Size: 8063 555
BsaAI yAC'GTr
Cuts at: 5971 5971
Size: 8618
BsaBI GATnn'nnATC
Cuts at: 6 5093 6132 8169 6
Size: 5087 1039 2037 455
Fragments arranged by size:
5087 2037 1039 455
BsaHI Gr'CG_yC
Cuts at: 130 6049 6297 6999 8495 130
Size: 5919 248 702 1496 253
Fragments arranged by size:
5919 1496 702 253 248
BsaWI w'CCGG_w
Cuts at: 135 5807 6328 7006 7280 7427 7838 135
Size: 5672 521 678 274 147 411 915
Fragments arranged by size:
5672 915 678 521 411 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 308 338 2279 2309 3792 3822 3898 3928 308
Size: 30 1941 30 1483 30 76 30 4998
Fragments arranged by size:
4998 1941 1483 76 30 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1018 2327 3862 3941 5355 1018
Size: 1309 1535 79 1414 4281
Fragments arranged by size:
4281 1535 1414 1309 79
BseYI C'CCAG_C
Cuts at: 933 1602 3039 3345 3585 6043 6801 7378 7874
Size: 669 1437 306 240 2458 758 577 496
Cuts at: 7874 933
Size: 1677
Fragments arranged by size:
2458 1677 1437 758 669 577 496 306
240
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1049 3460 4936 5309 1049
Size: 2411 1476 373 4358
Fragments arranged by size:
4358 2411 1476 373
BsiEI CG_ry'CG
Cuts at: 104 6160 6206 7414 104
Size: 6056 46 1208 1308
Fragments arranged by size:
6056 1308 1208 46
BsiHKAI G_wGCw'C
Cuts at: 3384 4066 5295 5632 6410 6600 7392 3384
Size: 682 1229 337 778 190 792 4610
Fragments arranged by size:
4610 1229 792 778 682 337 190
BsmI GAATG_Cn'
Cuts at: 3249 4645 3249
Size: 1396 7222
Bsp1286I G_dGCh'C
Cuts at: 3384 3539 4066 5275 5295 5632 6243 6336 6410
Size: 155 527 1209 20 337 611 93 74
Cuts at: 6410 6600 6662 7392 3384
Size: 190 62 730 4610
Fragments arranged by size:
4610 1209 730 611 527 337 190 155
93 74 62 20
BspEI T'CCGG_A
Cuts at: 135 7838 135
Size: 7703 915
BspHI T'CATG_A
Cuts at: 876 3882 7794 876
Size: 3006 3912 1700
Fragments arranged by size:
3912 3006 1700
BspMI ACCTGCnnnn'nnnn_
Cuts at: 2022 3171 3418 3666 5601 5668 5882 6184 6565
Size: 1149 247 248 1935 67 214 302 381
Cuts at: 6565 2022
Size: 4075
Fragments arranged by size:
4075 1935 1149 381 302 248 247 214
67
BsrBI CCG'CTC
Cuts at: 50 144 3719 6910 6964 8448 8613 50
Size: 94 3575 3191 54 1484 165 55
Fragments arranged by size:
3575 3191 1484 165 94 55 54
BsrDI GCAATG_nn'
Cuts at: 1017 1878 2389 2792 3512 3692 3951 6530 1017
Size: 861 511 403 720 180 259 2579 3105
Fragments arranged by size:
3105 2579 861 720 511 403 259 180
BsrFI r'CCGG_y
Cuts at: 6616 6797 7006 6616
Size: 181 209 8228
Fragments arranged by size:
8228 209 181
BsrGI T'GTAC_A
Cuts at: 2118 4860 4908 2118
Size: 2742 48 5828
Fragments arranged by size:
5828 2742 48
BssSI C'ACGA_G
Cuts at: 1778 6889 7247 1778
Size: 5111 358 3149
Fragments arranged by size:
5111 3149 358
BstAPI GCAn_nnn'nTGC
Cuts at: 678 1495 3778 5995 678
Size: 817 2283 2217 3301
Fragments arranged by size:
3301 2283 2217 817
BstBI TT'CG_AA
Cuts at: 3544 6979 3544
Size: 3435 5183
BstEII G'GTnAC_C
Cuts at: 3621 3621
Size: 8618
BstYI r'GATC_y
Cuts at: 1 243 693 751 2453 4788 4957 4974 5643
Size: 242 450 58 1702 2335 169 17 669
Cuts at: 5643 6110 6133 6468 6714 7726 7812 7824 8170
Size: 467 23 335 246 1012 86 12 346
Cuts at: 8170 1
Size: 449
Fragments arranged by size:
2335 1702 1012 669 467 450 449 346
335 246 242 169 86 58 23 17
12
BstZ17I GTA'TAC
Cuts at: 4399 4399
Size: 8618
Bsu36I CC'TnA_GG
Cuts at: 3392 3516 3392
Size: 124 8494
BtgI C'CryG_G
Cuts at: 107 554 107
Size: 447 8171
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 6568 6749 6912 6568
Size: 181 163 8274
Fragments arranged by size:
8274 181 163
BtsI GCAGTG_nn'
Cuts at: 983 5110 5861 983
Size: 4127 751 3740
Fragments arranged by size:
4127 3740 751
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 605 640 750 785 605
Size: 35 110 35 8438
Fragments arranged by size:
8438 110 35 35
DraI TTT'AAA
Cuts at: 210 374 1217 3989 5617 210
Size: 164 843 2772 1628 3211
Fragments arranged by size:
3211 2772 1628 843 164
DraIII CAC_nnn'GTG
Cuts at: 519 2439 3328 519
Size: 1920 889 5809
Fragments arranged by size:
5809 1920 889
DrdI GACnn_nn'nnGTC
Cuts at: 6324 7182 6324
Size: 858 7760
EaeI y'GGCC_r
Cuts at: 3410 6203 6377 6768 6795 8140 3410
Size: 2793 174 391 27 1345 3888
Fragments arranged by size:
3888 2793 1345 391 174 27
EagI C'GGCC_G
Cuts at: 6203 6203
Size: 8618
EarI CTCTTCn'nnn_
Cuts at: 323 1843 2972 6641 6851 323
Size: 1520 1129 3669 210 2090
Fragments arranged by size:
3669 2090 1520 1129 210
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 296 6826 7136 7282 8428 296
Size: 6530 310 146 1146 486
Fragments arranged by size:
6530 1146 486 310 146
EcoICRI GAG'CTC
Cuts at: 3382 5630 3382
Size: 2248 6370
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 952 1628 2186 2444 2687 3437 3683 4991 6443
Size: 676 558 258 243 750 246 1308 1452
Cuts at: 6443 6875 7622 7890 952
Size: 432 747 268 1680
Fragments arranged by size:
1680 1452 1308 750 747 676 558 432
268 258 246 243
EcoNI CCTnn'n_nnAGG
Cuts at: 2120 2120
Size: 8618
EcoO109I rG'GnC_Cy
Cuts at: 2317 5757 2317
Size: 3440 5178
EcoRI G'AATT_C
Cuts at: 2156 2268 2379 4476 4694 5622 2156
Size: 112 111 2097 218 928 5152
Fragments arranged by size:
5152 2097 928 218 112 111
EcoRV GAT'ATC
Cuts at: 4152 4277 4308 4152
Size: 125 31 8462
Fragments arranged by size:
8462 125 31
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 6637 6637
Size: 8618
FspI TGC'GCA
Cuts at: 6399 6399
Size: 8618
HaeII r_GCGC'y
Cuts at: 133 6052 6300 7322 8301 133
Size: 5919 248 1022 979 450
Fragments arranged by size:
5919 1022 979 450 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1305 1337 1926 1958 3898 3930 4538 4561 4570
Size: 32 589 32 1940 32 608 23 9
Cuts at: 4570 4593 4603 4635 6460 6490 6492 6522 1305
Size: 23 10 32 1825 30 2 30 3401
Fragments arranged by size:
3401 1940 1825 608 589 32 32 32
32 30 30 23 23 10 9 2
HincII GTy'rAC
Cuts at: 2563 5657 2563
Size: 3094 5524
HindIII A'AGCT_T
Cuts at: 3334 5525 3334
Size: 2191 6427
KasI G'GCGC_C
Cuts at: 129 6048 6296 129
Size: 5919 248 2451
Fragments arranged by size:
5919 2451 248
KpnI G_GTAC'C
Cuts at: 448 2446 5638 448
Size: 1998 3192 3428
Fragments arranged by size:
3428 3192 1998
MluI A'CGCG_T
Cuts at: 8051 8051
Size: 8618
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 503 2289 3717 5436 7289 7473 503
Size: 1786 1428 1719 1853 184 1648
Fragments arranged by size:
1853 1786 1719 1648 1428 184
MscI TGG'CCA
Cuts at: 3412 6379 3412
Size: 2967 5651
MslI CAynn'nnrTG
Cuts at: 1387 1438 1444 1889 1933 3881 4888 5318 6607
Size: 51 6 445 44 1948 1007 430 1289
Cuts at: 6607 6734 1387
Size: 127 3271
Fragments arranged by size:
3271 1948 1289 1007 445 430 127 51
44 6
MspA1I CmG'CkG
Cuts at: 960 1126 2620 2974 4906 5142 5422 5729 6043
Size: 166 1494 354 1932 236 280 307 314
Cuts at: 6043 6403 7416 7661 960
Size: 360 1013 245 1917
Fragments arranged by size:
1932 1917 1494 1013 360 354 314 307
280 245 236 166
NaeI GCC'GGC
Cuts at: 6799 6799
Size: 8618
NarI GG'CG_CC
Cuts at: 130 6049 6297 130
Size: 5919 248 2451
Fragments arranged by size:
5919 2451 248
NcoI C'CATG_G
Cuts at: 107 554 107
Size: 447 8171
NdeI CA'TA_TG
Cuts at: 2507 4857 2507
Size: 2350 6268
NgoMIV G'CCGG_C
Cuts at: 6797 6797
Size: 8618
NheI G'CTAG_C
Cuts at: 5505 5505
Size: 8618
NruI TCG'CGA
Cuts at: 289 289
Size: 8618
NsiI A_TGCA'T
Cuts at: 3974 3974
Size: 8618
NspI r_CATG'y
Cuts at: 1380 1443 1633 2540 3880 3976 4146 5671 6171
Size: 63 190 907 1340 96 170 1525 500
Cuts at: 6171 7078 1380
Size: 907 2920
Fragments arranged by size:
2920 1525 1340 907 907 500 190 170
96 63
PciI A'CATG_T
Cuts at: 1439 3876 7074 1439
Size: 2437 3198 2983
Fragments arranged by size:
3198 2983 2437
PflMI CCAn_nnn'nTGG
Cuts at: 4954 4954
Size: 8618
PfoI T'CCnGG_A
Cuts at: 164 1644 164
Size: 1480 7138
PmeI GTTT'AAAC
Cuts at: 5617 5617
Size: 8618
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 7757 7789 7757
Size: 32 8586
PpuMI rG'GwC_Cy
Cuts at: 2317 2317
Size: 8618
PshAI GACnn'nnGTC
Cuts at: 4013 4013
Size: 8618
PsiI TTA'TAA
Cuts at: 581 1811 581
Size: 1230 7388
PspXI vC'TCGA_Gb
Cuts at: 1363 1363
Size: 8618
PsrI GAACnnnnnnTACnnnnnnn_nnnnn'
Cuts at: 2111 2143 2111
Size: 32 8586
PstI C_TGCA'G
Cuts at: 2019 4756 5612 5665 6350 2019
Size: 2737 856 53 685 4287
Fragments arranged by size:
4287 2737 856 685 53
PvuI CG_AT'CG
Cuts at: 104 104
Size: 8618
PvuII CAG'CTG
Cuts at: 1126 2620 2974 4906 5422 6043 6403 1126
Size: 1494 354 1932 516 621 360 3341
Fragments arranged by size:
3341 1932 1494 621 516 360 354
RsrII CG'GwC_CG
Cuts at: 6813 6813
Size: 8618
SacI G_AGCT'C
Cuts at: 3384 5632 3384
Size: 2248 6370
SalI G'TCGA_C
Cuts at: 5655 5655
Size: 8618
SapI GCTCTTCn'nnn_
Cuts at: 2972 6641 6851 2972
Size: 3669 210 4739
Fragments arranged by size:
4739 3669 210
SbfI CC_TGCA'GG
Cuts at: 2019 5665 2019
Size: 3646 4972
ScaI AGT'ACT
Cuts at: 8135 8135
Size: 8618
SexAI A'CCwGG_T
Cuts at: 3324 3617 3324
Size: 293 8325
SfcI C'TryA_G
Cuts at: 33 1305 1562 2015 2255 2546 2726 2831 4752
Size: 1272 257 453 240 291 180 105 1921
Cuts at: 4752 4799 5264 5608 5661 6346 7339 7530 33
Size: 47 465 344 53 685 993 191 1121
Fragments arranged by size:
1921 1272 1121 993 685 465 453 344
291 257 240 191 180 105 53 47
SfoI GGC'GCC
Cuts at: 131 6050 6298 131
Size: 5919 248 2451
Fragments arranged by size:
5919 2451 248
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 8618
SmaI CCC'GGG
Cuts at: 5640 5640
Size: 8618
SmlI C'TyrA_G
Cuts at: 1363 1730 2772 3767 4011 5773 6688 7180 7442
Size: 367 1042 995 244 1762 915 492 262
Cuts at: 7442 1363
Size: 2539
Fragments arranged by size:
2539 1762 1042 995 915 492 367 262
244
SpeI A'CTAG_T
Cuts at: 67 67
Size: 8618
SphI G_CATG'C
Cuts at: 5671 6171 5671
Size: 500 8118
SspI AAT'ATT
Cuts at: 1119 2688 3557 5432 5442 5452 1119
Size: 1569 869 1875 10 10 4285
Fragments arranged by size:
4285 1875 1569 869 10 10
StuI AGG'CCT
Cuts at: 1100 1100
Size: 8618
StyI C'CwwG_G
Cuts at: 107 554 3600 5752 6106 107
Size: 447 3046 2152 354 2619
Fragments arranged by size:
3046 2619 2152 447 354
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 217 1532 4366 4607 6201 6334 6793 7002 217
Size: 1315 2834 241 1594 133 459 209 1833
Fragments arranged by size:
2834 1833 1594 1315 459 241 209 133
TatI w'GTAC_w
Cuts at: 1937 2118 2350 2520 2539 2706 3189 3213 3525
Size: 181 232 170 19 167 483 24 312
Cuts at: 3525 3747 3796 4001 4860 4908 8133 1937
Size: 222 49 205 859 48 3225 2422
Fragments arranged by size:
3225 2422 859 483 312 232 222 205
181 170 167 49 48 24 19
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 1507 1539 8135 8167 1507
Size: 32 6596 32 1958
Fragments arranged by size:
6596 1958 32 32
Tth111I GACn'n_nGTC
Cuts at: 6415 6415
Size: 8618
XbaI T'CTAG_A
Cuts at: 5649 5649
Size: 8618
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 3607 5517 6113 3607
Size: 1910 596 6112
Fragments arranged by size:
6112 1910 596
XhoI C'TCGA_G
Cuts at: 1363 1730 1363
Size: 367 8251
XmaI C'CCGG_G
Cuts at: 5638 5638
Size: 8618
XmnI GAAnn'nnTTC
Cuts at: 351 993 351
Size: 642 7976
Enzymes that do cut:
AarI AccI Acc65I AclI AcuI AflIII AgeI AhdI
AlfI AloI AlwNI ApaLI ApoI AseI AsiSI AvaI
BaeI BamHI BanI BanII BbeI BbsI BbvCI BciVI
BclI BfrBI BglII BlpI Bme1580I BmgBI BmrI BmtI
BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI
BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI
Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI
BssSI BstAPI BstBI BstEII BstYI BstZ17I Bsu36I BtgI
BtgZI BtsI CspCI DraI DraIII DrdI EaeI EagI
EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI EcoRV
FalI FspI HaeII Hin4I HincII HindIII KasI KpnI
MluI MmeI MscI MslI MspA1I NaeI NarI NcoI
NdeI NgoMIV NheI NruI NsiI NspI PciI PflMI
PfoI PmeI PpiI PpuMI PshAI PsiI PspXI PsrI
PstI PvuI PvuII RsrII SacI SalI SapI SbfI
ScaI SexAI SfcI SfoI SgfI SmaI SmlI SpeI
SphI SspI StuI StyI TaqII TatI TstI Tth111I
XbaI XcmI XhoI XmaI XmnI
Enzymes that do not cut:
AatII AfeI AflII AleI ApaI AscI AvrII BcgI
BglI BsiWI BsmBI BssHII BstXI ClaI FseI FspAI
HpaI MfeI NotI PacI PasI PmlI PspOMI SacII
SanDI SfiI SgrAI SnaBI SrfI SwaI ZraI