Restriction map for
pF1KE0099
(Circular) (Six-base) MAPSORT of: pF1KE0099.seq Check: 9154 from: 1 to: 3914
pF1KE0099 3914 bp
With 182 enzymes: SgfI *
April 24, 2009 13:10 ..
AccI GT'mk_AC
Cuts at: 952 952
Size: 3914
Acc65I G'GTAC_C
Cuts at: 930 930
Size: 3914
AclI AA'CG_TT
Cuts at: 3754 3754
Size: 3914
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 333 1739 2171 2918 3186 333
Size: 1406 432 747 268 1061
Fragments arranged by size:
1406 1061 747 432 268
AfeI AGC'GCT
Cuts at: 697 873 697
Size: 176 3738
AflIII A'CryG_T
Cuts at: 1266 2370 3347 1266
Size: 1104 977 1833
Fragments arranged by size:
1833 1104 977
AgeI A'CCGG_T
Cuts at: 2302 2302
Size: 3914
AhdI GACnn_n'nnGTC
Cuts at: 267 267
Size: 3914
AlwNI CAG_nnn'CTG
Cuts at: 579 1219 2786 579
Size: 640 1567 1707
Fragments arranged by size:
1707 1567 640
ApaLI G'TGCA_C
Cuts at: 2684 2684
Size: 3914
ApoI r'AATT_y
Cuts at: 918 3267 3523 918
Size: 2349 256 1309
Fragments arranged by size:
2349 1309 256
AseI AT'TA_AT
Cuts at: 20 20
Size: 3914
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 3914
AvaI C'yCGr_G
Cuts at: 811 877 934 811
Size: 66 57 3791
Fragments arranged by size:
3791 66 57
BamHI G'GATC_C
Cuts at: 939 939
Size: 3914
BanI G'GyrC_C
Cuts at: 736 930 1344 1592 1627 736
Size: 194 414 248 35 3023
Fragments arranged by size:
3023 414 248 194 35
BanII G_rGCy'C
Cuts at: 199 521 557 607 928 1958 199
Size: 322 36 50 321 1030 2155
Fragments arranged by size:
2155 1030 322 321 50 36
BbeI G_GCGC'C
Cuts at: 740 1348 1596 740
Size: 608 248 3058
Fragments arranged by size:
3058 608 248
BciVI GTATCCnnnnn_n'
Cuts at: 1812 2573 3914 1812
Size: 761 1341 1812
Fragments arranged by size:
1812 1341 761
BclI T'GATC_A
Cuts at: 1434 1434
Size: 3914
BglI GCCn_nnn'nGGC
Cuts at: 611 745 611
Size: 134 3780
BglII A'GATC_T
Cuts at: 1429 3466 1429
Size: 2037 1877
BlpI GC'TnA_GC
Cuts at: 601 787 1026 601
Size: 186 239 3489
Fragments arranged by size:
3489 239 186
Bme1580I G_kGCm'C
Cuts at: 1539 1632 2688 1539
Size: 93 1056 2765
Fragments arranged by size:
2765 1056 93
BmrI ACTGGGnnnn_n'
Cuts at: 267 336 619 1126 1207 1542 3686 267
Size: 69 283 507 81 335 2144 495
Fragments arranged by size:
2144 507 495 335 283 81 69
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 176 2281 176
Size: 2105 1809
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 469 865 469
Size: 396 3518
Bpu10I CC'TnA_GC
Cuts at: 868 868
Size: 3914
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 706 733 1090 1969 2461 2759 706
Size: 27 357 879 492 298 1861
Fragments arranged by size:
1861 879 492 357 298 27
BsaI GGTCTCn'nnnn_
Cuts at: 3618 3618
Size: 3914
BsaAI yAC'GTr
Cuts at: 234 1267 234
Size: 1033 2881
BsaBI GATnn'nnATC
Cuts at: 6 1428 3465 6
Size: 1422 2037 455
Fragments arranged by size:
2037 1422 455
BsaHI Gr'CG_yC
Cuts at: 737 1345 1593 2295 3791 737
Size: 608 248 702 1496 860
Fragments arranged by size:
1496 860 702 608 248
BsaWI w'CCGG_w
Cuts at: 1103 1624 2302 2576 2723 3134 1103
Size: 521 678 274 147 411 1883
Fragments arranged by size:
1883 678 521 411 274 147
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 466 523 631 895 466
Size: 57 108 264 3485
Fragments arranged by size:
3485 264 108 57
BseYI C'CCAG_C
Cuts at: 1339 2097 2674 3170 1339
Size: 758 577 496 2083
Fragments arranged by size:
2083 758 577 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 490 565 490
Size: 75 3839
BsiEI CG_ry'CG
Cuts at: 104 630 1456 1502 2710 104
Size: 526 826 46 1208 1308
Fragments arranged by size:
1308 1208 826 526 46
BsiHKAI G_wGCw'C
Cuts at: 140 245 521 557 784 928 1706 1896 2688
Size: 105 276 36 227 144 778 190 792
Cuts at: 2688 140
Size: 1366
Fragments arranged by size:
1366 792 778 276 227 190 144 105
36
Bsp1286I G_dGCh'C
Cuts at: 140 199 245 521 557 607 784 928 1539
Size: 59 46 276 36 50 177 144 611
Cuts at: 1539 1632 1706 1896 1958 2688 140
Size: 93 74 190 62 730 1366
Fragments arranged by size:
1366 730 611 276 190 177 144 93
74 62 59 50 46 36
BspEI T'CCGG_A
Cuts at: 3134 3134
Size: 3914
BspHI T'CATG_A
Cuts at: 3090 3090
Size: 3914
BspMI ACCTGCnnnn'nnnn_
Cuts at: 964 1178 1480 1861 964
Size: 214 302 381 3017
Fragments arranged by size:
3017 381 302 214
BsrBI CCG'CTC
Cuts at: 50 2206 2260 3744 3909 50
Size: 2156 54 1484 165 55
Fragments arranged by size:
2156 1484 165 55 54
BsrDI GCAATG_nn'
Cuts at: 1826 1826
Size: 3914
BsrFI r'CCGG_y
Cuts at: 1912 2093 2302 1912
Size: 181 209 3524
Fragments arranged by size:
3524 209 181
BssHII G'CGCG_C
Cuts at: 546 566 568 632 634 636 706 546
Size: 20 2 64 2 2 70 3754
Fragments arranged by size:
3754 70 64 20 2 2 2
BssSI C'ACGA_G
Cuts at: 583 2185 2543 583
Size: 1602 358 1954
Fragments arranged by size:
1954 1602 358
BstAPI GCAn_nnn'nTGC
Cuts at: 588 1291 588
Size: 703 3211
BstBI TT'CG_AA
Cuts at: 2275 2275
Size: 3914
BstYI r'GATC_y
Cuts at: 1 444 939 1406 1429 1764 2010 3022 3108
Size: 443 495 467 23 335 246 1012 86
Cuts at: 3108 3120 3466 1
Size: 12 346 449
Fragments arranged by size:
1012 495 467 449 443 346 335 246
86 23 12
Bsu36I CC'TnA_GG
Cuts at: 424 424
Size: 3914
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 1864 2045 2208 1864
Size: 181 163 3570
Fragments arranged by size:
3570 181 163
BtsI GCAGTG_nn'
Cuts at: 1157 1157
Size: 3914
DraI TTT'AAA
Cuts at: 913 913
Size: 3914
DrdI GACnn_nn'nnGTC
Cuts at: 1620 2478 1620
Size: 858 3056
EaeI y'GGCC_r
Cuts at: 131 221 701 746 1499 1673 2064 2091 3436
Size: 90 480 45 753 174 391 27 1345
Cuts at: 3436 131
Size: 609
Fragments arranged by size:
1345 753 609 480 391 174 90 45
27
EagI C'GGCC_G
Cuts at: 1499 1499
Size: 3914
EarI CTCTTCn'nnn_
Cuts at: 145 1937 2147 145
Size: 1792 210 1912
Fragments arranged by size:
1912 1792 210
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 276 811 2122 2432 2578 3724 276
Size: 535 1311 310 146 1146 466
Fragments arranged by size:
1311 1146 535 466 310 146
EcoICRI GAG'CTC
Cuts at: 519 555 926 519
Size: 36 371 3507
Fragments arranged by size:
3507 371 36
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 333 469 865 1739 2171 2918 3186 333
Size: 136 396 874 432 747 268 1061
Fragments arranged by size:
1061 874 747 432 396 268 136
EcoNI CCTnn'n_nnAGG
Cuts at: 406 427 406
Size: 21 3893
EcoO109I rG'GnC_Cy
Cuts at: 216 420 816 1053 216
Size: 204 396 237 3077
Fragments arranged by size:
3077 396 237 204
EcoRI G'AATT_C
Cuts at: 918 918
Size: 3914
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 879 1933 879
Size: 1054 2860
FspI TGC'GCA
Cuts at: 658 1695 658
Size: 1037 2877
HaeII r_GCGC'y
Cuts at: 699 740 875 1348 1596 2618 3597 699
Size: 41 135 473 248 1022 979 1016
Fragments arranged by size:
1022 1016 979 473 248 135 41
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 253 285 429 461 1756 1786 1788 1818 253
Size: 32 144 32 1295 30 2 30 2349
Fragments arranged by size:
2349 1295 144 32 32 30 30 2
HincII GTy'rAC
Cuts at: 953 953
Size: 3914
KasI G'GCGC_C
Cuts at: 736 1344 1592 736
Size: 608 248 3058
Fragments arranged by size:
3058 608 248
KpnI G_GTAC'C
Cuts at: 934 934
Size: 3914
MluI A'CGCG_T
Cuts at: 3347 3347
Size: 3914
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 2585 2769 2585
Size: 184 3730
MscI TGG'CCA
Cuts at: 223 1675 223
Size: 1452 2462
MslI CAynn'nnrTG
Cuts at: 1903 2030 1903
Size: 127 3787
MspA1I CmG'CkG
Cuts at: 254 543 1025 1339 1699 2712 2957 254
Size: 289 482 314 360 1013 245 1211
Fragments arranged by size:
1211 1013 482 360 314 289 245
NaeI GCC'GGC
Cuts at: 2095 2095
Size: 3914
NarI GG'CG_CC
Cuts at: 737 1345 1593 737
Size: 608 248 3058
Fragments arranged by size:
3058 608 248
NgoMIV G'CCGG_C
Cuts at: 2093 2093
Size: 3914
NspI r_CATG'y
Cuts at: 967 1467 2374 967
Size: 500 907 2507
Fragments arranged by size:
2507 907 500
PasI CC'CwG_GG
Cuts at: 200 200
Size: 3914
PciI A'CATG_T
Cuts at: 2370 2370
Size: 3914
PflMI CCAn_nnn'nTGG
Cuts at: 534 699 534
Size: 165 3749
PmeI GTTT'AAAC
Cuts at: 913 913
Size: 3914
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3053 3085 3053
Size: 32 3882
PpuMI rG'GwC_Cy
Cuts at: 216 816 216
Size: 600 3314
PspXI vC'TCGA_Gb
Cuts at: 811 811
Size: 3914
PstI C_TGCA'G
Cuts at: 961 1646 961
Size: 685 3229
PvuI CG_AT'CG
Cuts at: 104 104
Size: 3914
PvuII CAG'CTG
Cuts at: 1339 1699 1339
Size: 360 3554
RsrII CG'GwC_CG
Cuts at: 2109 2109
Size: 3914
SacI G_AGCT'C
Cuts at: 521 557 928 521
Size: 36 371 3507
Fragments arranged by size:
3507 371 36
SalI G'TCGA_C
Cuts at: 951 951
Size: 3914
SapI GCTCTTCn'nnn_
Cuts at: 145 1937 2147 145
Size: 1792 210 1912
Fragments arranged by size:
1912 1792 210
SbfI CC_TGCA'GG
Cuts at: 961 961
Size: 3914
ScaI AGT'ACT
Cuts at: 3431 3431
Size: 3914
SfcI C'TryA_G
Cuts at: 33 675 957 1642 2635 2826 33
Size: 642 282 685 993 191 1121
Fragments arranged by size:
1121 993 685 642 282 191
SfoI GGC'GCC
Cuts at: 738 1346 1594 738
Size: 608 248 3058
Fragments arranged by size:
3058 608 248
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 3914
SmaI CCC'GGG
Cuts at: 936 936
Size: 3914
SmlI C'TyrA_G
Cuts at: 712 721 811 877 1069 1984 2476 2738 712
Size: 9 90 66 192 915 492 262 1888
Fragments arranged by size:
1888 915 492 262 192 90 66 9
SpeI A'CTAG_T
Cuts at: 67 67
Size: 3914
SphI G_CATG'C
Cuts at: 967 1467 967
Size: 500 3414
StuI AGG'CCT
Cuts at: 830 830
Size: 3914
StyI C'CwwG_G
Cuts at: 128 290 464 761 825 1048 1402 128
Size: 162 174 297 64 223 354 2640
Fragments arranged by size:
2640 354 297 223 174 162 64
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 919 1497 1630 2089 2298 919
Size: 578 133 459 209 2535
Fragments arranged by size:
2535 578 459 209 133
TatI w'GTAC_w
Cuts at: 884 3429 884
Size: 2545 1369
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 3431 3463 3431
Size: 32 3882
Tth111I GACn'n_nGTC
Cuts at: 1711 1711
Size: 3914
XbaI T'CTAG_A
Cuts at: 945 945
Size: 3914
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 231 1409 231
Size: 1178 2736
XhoI C'TCGA_G
Cuts at: 811 877 811
Size: 66 3848
XmaI C'CCGG_G
Cuts at: 934 934
Size: 3914
Enzymes that do cut:
AccI Acc65I AclI AcuI AfeI AflIII AgeI AhdI
AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI BanI
BanII BbeI BciVI BclI BglI BglII BlpI Bme1580I
BmrI BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI
BsaHI BsaWI BseRI BseYI BsgI BsiEI BsiHKAI Bsp1286I
BspEI BspHI BspMI BsrBI BsrDI BsrFI BssHII BssSI
BstAPI BstBI BstYI Bsu36I BtgZI BtsI DraI DrdI
EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I
EcoRI FalI FspI HaeII Hin4I HincII KasI KpnI
MluI MmeI MscI MslI MspA1I NaeI NarI NgoMIV
NspI PasI PciI PflMI PmeI PpiI PpuMI PspXI
PstI PvuI PvuII RsrII SacI SalI SapI SbfI
ScaI SfcI SfoI SgfI SmaI SmlI SpeI SphI
StuI StyI TaqII TatI TstI Tth111I XbaI XcmI
XhoI XmaI
Enzymes that do not cut:
AarI AatII AflII AleI AlfI AloI ApaI AscI
AvrII BaeI BbsI BbvCI BcgI BfrBI BmgBI BmtI
BsaXI BsiWI BsmI BsmBI BsrGI BstEII BstXI BstZ17I
BtgI ClaI CspCI DraIII EcoRV FseI FspAI HindIII
HpaI MfeI NcoI NdeI NheI NotI NruI NsiI
PacI PfoI PmlI PshAI PsiI PspOMI PsrI SacII
SanDI SexAI SfiI SgrAI SnaBI SrfI SspI SwaI
XmnI ZraI