Restriction map for
pF1KE0101
(Circular) (Six-base) MAPSORT of: pF1KE0101.seq Check: 7925 from: 1 to: 3659
pF1KE0101 3659 bp
With 182 enzymes: SgfI *
April 24, 2009 13:10 ..
AccI GT'mk_AC
Cuts at: 697 697
Size: 3659
Acc65I G'GTAC_C
Cuts at: 675 675
Size: 3659
AclI AA'CG_TT
Cuts at: 3499 3499
Size: 3659
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1484 1916 2663 2931 1484
Size: 432 747 268 2212
Fragments arranged by size:
2212 747 432 268
AflIII A'CryG_T
Cuts at: 1011 2115 3092 1011
Size: 1104 977 1578
Fragments arranged by size:
1578 1104 977
AgeI A'CCGG_T
Cuts at: 146 2047 146
Size: 1901 1758
AleI CACnn'nnGTG
Cuts at: 147 595 147
Size: 448 3211
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 175 175
Size: 3659
AlwNI CAG_nnn'CTG
Cuts at: 508 964 2531 508
Size: 456 1567 1636
Fragments arranged by size:
1636 1567 456
ApaLI G'TGCA_C
Cuts at: 2429 2429
Size: 3659
ApoI r'AATT_y
Cuts at: 169 197 487 663 3012 3268 169
Size: 28 290 176 2349 256 560
Fragments arranged by size:
2349 560 290 256 176 28
AseI AT'TA_AT
Cuts at: 20 497 20
Size: 477 3182
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 3659
AvaI C'yCGr_G
Cuts at: 444 679 444
Size: 235 3424
BamHI G'GATC_C
Cuts at: 684 684
Size: 3659
BanI G'GyrC_C
Cuts at: 186 675 1089 1337 1372 186
Size: 489 414 248 35 2473
Fragments arranged by size:
2473 489 414 248 35
BanII G_rGCy'C
Cuts at: 673 1703 673
Size: 1030 2629
BbeI G_GCGC'C
Cuts at: 1093 1341 1093
Size: 248 3411
BciVI GTATCCnnnnn_n'
Cuts at: 1557 2318 3659 1557
Size: 761 1341 1557
Fragments arranged by size:
1557 1341 761
BclI T'GATC_A
Cuts at: 1179 1179
Size: 3659
BglI GCCn_nnn'nGGC
Cuts at: 517 517
Size: 3659
BglII A'GATC_T
Cuts at: 129 1174 3211 129
Size: 1045 2037 577
Fragments arranged by size:
2037 1045 577
BlpI GC'TnA_GC
Cuts at: 402 771 402
Size: 369 3290
Bme1580I G_kGCm'C
Cuts at: 1284 1377 2433 1284
Size: 93 1056 2510
Fragments arranged by size:
2510 1056 93
BmrI ACTGGGnnnn_n'
Cuts at: 524 871 952 1287 3431 524
Size: 347 81 335 2144 752
Fragments arranged by size:
2144 752 347 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2026 2026
Size: 3659
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 238 835 1714 2206 2504 238
Size: 597 879 492 298 1393
Fragments arranged by size:
1393 879 597 492 298
BsaI GGTCTCn'nnnn_
Cuts at: 3363 3363
Size: 3659
BsaAI yAC'GTr
Cuts at: 1012 1012
Size: 3659
BsaBI GATnn'nnATC
Cuts at: 6 302 568 1173 3210 6
Size: 296 266 605 2037 455
Fragments arranged by size:
2037 605 455 296 266
BsaHI Gr'CG_yC
Cuts at: 1090 1338 2040 3536 1090
Size: 248 702 1496 1213
Fragments arranged by size:
1496 1213 702 248
BsaWI w'CCGG_w
Cuts at: 146 848 1369 2047 2321 2468 2879 146
Size: 702 521 678 274 147 411 926
Fragments arranged by size:
926 702 678 521 411 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 605 635 605
Size: 30 3629
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 302 302
Size: 3659
BseYI C'CCAG_C
Cuts at: 1084 1842 2419 2915 1084
Size: 758 577 496 1828
Fragments arranged by size:
1828 758 577 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 118 198 118
Size: 80 3579
BsiEI CG_ry'CG
Cuts at: 104 1201 1247 2455 104
Size: 1097 46 1208 1308
Fragments arranged by size:
1308 1208 1097 46
BsiHKAI G_wGCw'C
Cuts at: 673 1451 1641 2433 673
Size: 778 190 792 1899
Fragments arranged by size:
1899 792 778 190
Bsp1286I G_dGCh'C
Cuts at: 673 1284 1377 1451 1641 1703 2433 673
Size: 611 93 74 190 62 730 1899
Fragments arranged by size:
1899 730 611 190 93 74 62
BspEI T'CCGG_A
Cuts at: 2879 2879
Size: 3659
BspHI T'CATG_A
Cuts at: 2835 2835
Size: 3659
BspMI ACCTGCnnnn'nnnn_
Cuts at: 709 923 1225 1606 709
Size: 214 302 381 2762
Fragments arranged by size:
2762 381 302 214
BsrBI CCG'CTC
Cuts at: 50 1951 2005 3489 3654 50
Size: 1901 54 1484 165 55
Fragments arranged by size:
1901 1484 165 55 54
BsrDI GCAATG_nn'
Cuts at: 1571 1571
Size: 3659
BsrFI r'CCGG_y
Cuts at: 146 1657 1838 2047 146
Size: 1511 181 209 1758
Fragments arranged by size:
1758 1511 209 181
BssSI C'ACGA_G
Cuts at: 1930 2288 1930
Size: 358 3301
BstAPI GCAn_nnn'nTGC
Cuts at: 526 1036 526
Size: 510 3149
BstBI TT'CG_AA
Cuts at: 2020 2020
Size: 3659
BstEII G'GTnAC_C
Cuts at: 588 588
Size: 3659
BstYI r'GATC_y
Cuts at: 1 129 684 1151 1174 1509 1755 2767 2853
Size: 128 555 467 23 335 246 1012 86
Cuts at: 2853 2865 3211 1
Size: 12 346 449
Fragments arranged by size:
1012 555 467 449 346 335 246 128
86 23 12
BtgI C'CryG_G
Cuts at: 363 382 363
Size: 19 3640
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 1609 1790 1953 1609
Size: 181 163 3315
Fragments arranged by size:
3315 181 163
BtsI GCAGTG_nn'
Cuts at: 902 902
Size: 3659
DraI TTT'AAA
Cuts at: 658 658
Size: 3659
DrdI GACnn_nn'nnGTC
Cuts at: 1365 2223 1365
Size: 858 2801
EaeI y'GGCC_r
Cuts at: 395 1244 1418 1809 1836 3181 395
Size: 849 174 391 27 1345 873
Fragments arranged by size:
1345 873 849 391 174 27
EagI C'GGCC_G
Cuts at: 1244 1244
Size: 3659
EarI CTCTTCn'nnn_
Cuts at: 1682 1892 1682
Size: 210 3449
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 1867 2177 2323 3469 1867
Size: 310 146 1146 2057
Fragments arranged by size:
2057 1146 310 146
EcoICRI GAG'CTC
Cuts at: 671 671
Size: 3659
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1484 1916 2663 2931 1484
Size: 432 747 268 2212
Fragments arranged by size:
2212 747 432 268
EcoO109I rG'GnC_Cy
Cuts at: 798 798
Size: 3659
EcoRI G'AATT_C
Cuts at: 663 663
Size: 3659
EcoRV GAT'ATC
Cuts at: 570 570
Size: 3659
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 1678 1678
Size: 3659
FspI TGC'GCA
Cuts at: 1440 1440
Size: 3659
HaeII r_GCGC'y
Cuts at: 1093 1341 2363 3342 1093
Size: 248 1022 979 1410
Fragments arranged by size:
1410 1022 979 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1501 1531 1533 1563 1501
Size: 30 2 30 3597
Fragments arranged by size:
3597 30 30 2
HincII GTy'rAC
Cuts at: 698 698
Size: 3659
KasI G'GCGC_C
Cuts at: 1089 1337 1089
Size: 248 3411
KpnI G_GTAC'C
Cuts at: 679 679
Size: 3659
MluI A'CGCG_T
Cuts at: 3092 3092
Size: 3659
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 318 2330 2514 318
Size: 2012 184 1463
Fragments arranged by size:
2012 1463 184
MscI TGG'CCA
Cuts at: 397 1420 397
Size: 1023 2636
MslI CAynn'nnrTG
Cuts at: 112 147 595 1648 1775 112
Size: 35 448 1053 127 1996
Fragments arranged by size:
1996 1053 448 127 35
MspA1I CmG'CkG
Cuts at: 770 1084 1444 2457 2702 770
Size: 314 360 1013 245 1727
Fragments arranged by size:
1727 1013 360 314 245
NaeI GCC'GGC
Cuts at: 1840 1840
Size: 3659
NarI GG'CG_CC
Cuts at: 1090 1338 1090
Size: 248 3411
NcoI C'CATG_G
Cuts at: 363 382 363
Size: 19 3640
NgoMIV G'CCGG_C
Cuts at: 1838 1838
Size: 3659
NspI r_CATG'y
Cuts at: 712 1212 2119 712
Size: 500 907 2252
Fragments arranged by size:
2252 907 500
PciI A'CATG_T
Cuts at: 2115 2115
Size: 3659
PmeI GTTT'AAAC
Cuts at: 658 658
Size: 3659
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2798 2830 2798
Size: 32 3627
PstI C_TGCA'G
Cuts at: 706 1391 706
Size: 685 2974
PvuI CG_AT'CG
Cuts at: 104 104
Size: 3659
PvuII CAG'CTG
Cuts at: 1084 1444 1084
Size: 360 3299
RsrII CG'GwC_CG
Cuts at: 1854 1854
Size: 3659
SacI G_AGCT'C
Cuts at: 673 673
Size: 3659
SalI G'TCGA_C
Cuts at: 696 696
Size: 3659
SapI GCTCTTCn'nnn_
Cuts at: 1682 1892 1682
Size: 210 3449
SbfI CC_TGCA'GG
Cuts at: 706 706
Size: 3659
ScaI AGT'ACT
Cuts at: 3176 3176
Size: 3659
SfcI C'TryA_G
Cuts at: 33 581 702 1387 2380 2571 33
Size: 548 121 685 993 191 1121
Fragments arranged by size:
1121 993 685 548 191 121
SfoI GGC'GCC
Cuts at: 1091 1339 1091
Size: 248 3411
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 3659
SgrAI Cr'CCGG_yG
Cuts at: 146 146
Size: 3659
SmaI CCC'GGG
Cuts at: 681 681
Size: 3659
SmlI C'TyrA_G
Cuts at: 253 814 1729 2221 2483 253
Size: 561 915 492 262 1429
Fragments arranged by size:
1429 915 561 492 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 3659
SphI G_CATG'C
Cuts at: 712 1212 712
Size: 500 3159
StyI C'CwwG_G
Cuts at: 363 382 530 793 1147 363
Size: 19 148 263 354 2875
Fragments arranged by size:
2875 354 263 148 19
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 1242 1375 1834 2043 1242
Size: 133 459 209 2858
Fragments arranged by size:
2858 459 209 133
TatI w'GTAC_w
Cuts at: 3174 3174
Size: 3659
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 605 637 3176 3208 605
Size: 32 2539 32 1056
Fragments arranged by size:
2539 1056 32 32
Tth111I GACn'n_nGTC
Cuts at: 1456 1456
Size: 3659
XbaI T'CTAG_A
Cuts at: 690 690
Size: 3659
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 114 1154 114
Size: 1040 2619
XmaI C'CCGG_G
Cuts at: 679 679
Size: 3659
Enzymes that do cut:
AccI Acc65I AclI AcuI AflIII AgeI AleI AlfI
AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI BanI
BanII BbeI BciVI BclI BglI BglII BlpI Bme1580I
BmrI BplI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI
BsaXI BseRI BseYI BsgI BsiEI BsiHKAI Bsp1286I BspEI
BspHI BspMI BsrBI BsrDI BsrFI BssSI BstAPI BstBI
BstEII BstYI BtgI BtgZI BtsI DraI DrdI EaeI
EagI EarI EciI EcoICRI Eco57MI EcoO109I EcoRI EcoRV
FalI FspI HaeII Hin4I HincII KasI KpnI MluI
MmeI MscI MslI MspA1I NaeI NarI NcoI NgoMIV
NspI PciI PmeI PpiI PstI PvuI PvuII RsrII
SacI SalI SapI SbfI ScaI SfcI SfoI SgfI
SgrAI SmaI SmlI SpeI SphI StyI TaqII TatI
TstI Tth111I XbaI XcmI XmaI
Enzymes that do not cut:
AarI AatII AfeI AflII AhdI AloI ApaI AscI
AvrII BaeI BbsI BbvCI BcgI BfrBI BmgBI BmtI
BpmI Bpu10I BsiWI BsmI BsmBI BsrGI BssHII BstXI
BstZ17I Bsu36I ClaI CspCI DraIII EcoNI FseI FspAI
HindIII HpaI MfeI NdeI NheI NotI NruI NsiI
PacI PasI PflMI PfoI PmlI PpuMI PshAI PsiI
PspOMI PspXI PsrI SacII SanDI SexAI SfiI SnaBI
SrfI SspI StuI SwaI XhoI XmnI ZraI