Restriction map for
pF1KE0102
(Circular) (Six-base) MAPSORT of: pF1KE0102.seq Check: 6809 from: 1 to: 4190
pF1KE0102 4190 bp
With 182 enzymes: SgfI *
March 2, 2010 14:30 ..
AccI GT'mk_AC
Cuts at: 1228 1228
Size: 4190
Acc65I G'GTAC_C
Cuts at: 1206 1206
Size: 4190
AclI AA'CG_TT
Cuts at: 4030 4030
Size: 4190
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 571 2015 2447 3194 3462 571
Size: 1444 432 747 268 1299
Fragments arranged by size:
1444 1299 747 432 268
AflIII A'CryG_T
Cuts at: 1542 2646 3623 1542
Size: 1104 977 2109
Fragments arranged by size:
2109 1104 977
AgeI A'CCGG_T
Cuts at: 2578 2578
Size: 4190
AleI CACnn'nnGTG
Cuts at: 499 499
Size: 4190
AlwNI CAG_nnn'CTG
Cuts at: 1158 1495 3062 1158
Size: 337 1567 2286
Fragments arranged by size:
2286 1567 337
ApaI G_GGCC'C
Cuts at: 769 769
Size: 4190
ApaLI G'TGCA_C
Cuts at: 2960 2960
Size: 4190
ApoI r'AATT_y
Cuts at: 1194 3543 3799 1194
Size: 2349 256 1585
Fragments arranged by size:
2349 1585 256
AseI AT'TA_AT
Cuts at: 20 20
Size: 4190
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 4190
AvaI C'yCGr_G
Cuts at: 1210 1210
Size: 4190
BamHI G'GATC_C
Cuts at: 1034 1215 1034
Size: 181 4009
BanI G'GyrC_C
Cuts at: 163 379 424 463 628 706 1051 1206 1620
Size: 216 45 39 165 78 345 155 414
Cuts at: 1620 1868 1903 163
Size: 248 35 2450
Fragments arranged by size:
2450 414 345 248 216 165 155 78
45 39 35
BanII G_rGCy'C
Cuts at: 576 769 1204 2234 576
Size: 193 435 1030 2532
Fragments arranged by size:
2532 1030 435 193
BbeI G_GCGC'C
Cuts at: 1624 1872 1624
Size: 248 3942
BciVI GTATCCnnnnn_n'
Cuts at: 2088 2849 4190 2088
Size: 761 1341 2088
Fragments arranged by size:
2088 1341 761
BclI T'GATC_A
Cuts at: 641 1118 1710 641
Size: 477 592 3121
Fragments arranged by size:
3121 592 477
BglII A'GATC_T
Cuts at: 1705 3742 1705
Size: 2037 2153
BlpI GC'TnA_GC
Cuts at: 680 1302 680
Size: 622 3568
Bme1580I G_kGCm'C
Cuts at: 373 384 466 631 769 791 1054 1815 1908
Size: 11 82 165 138 22 263 761 93
Cuts at: 1908 2964 373
Size: 1056 1599
Fragments arranged by size:
1599 1056 761 263 165 138 93 82
22 11
BmgBI CAC'GTC
Cuts at: 207 939 207
Size: 732 3458
BmrI ACTGGGnnnn_n'
Cuts at: 1402 1483 1818 3962 1402
Size: 81 335 2144 1630
Fragments arranged by size:
2144 1630 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2557 2557
Size: 4190
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 127 252 373 498 673 127
Size: 125 121 125 175 3644
Fragments arranged by size:
3644 175 125 125 121
Bpu10I CC'TnA_GC
Cuts at: 731 731
Size: 4190
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1366 2245 2737 3035 1366
Size: 879 492 298 2521
Fragments arranged by size:
2521 879 492 298
BsaI GGTCTCn'nnnn_
Cuts at: 3894 3894
Size: 4190
BsaAI yAC'GTr
Cuts at: 1543 1543
Size: 4190
BsaBI GATnn'nnATC
Cuts at: 6 1704 3741 6
Size: 1698 2037 455
Fragments arranged by size:
2037 1698 455
BsaHI Gr'CG_yC
Cuts at: 1621 1869 2571 4067 1621
Size: 248 702 1496 1744
Fragments arranged by size:
1744 1496 702 248
BsaWI w'CCGG_w
Cuts at: 1379 1900 2578 2852 2999 3410 1379
Size: 521 678 274 147 411 2159
Fragments arranged by size:
2159 678 521 411 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 346 376 346
Size: 30 4160
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 294 294
Size: 4190
BseYI C'CCAG_C
Cuts at: 702 756 761 1615 2373 2950 3446 702
Size: 54 5 854 758 577 496 1446
Fragments arranged by size:
1446 854 758 577 496 54 5
BsiEI CG_ry'CG
Cuts at: 104 1732 1778 2986 104
Size: 1628 46 1208 1308
Fragments arranged by size:
1628 1308 1208 46
BsiHKAI G_wGCw'C
Cuts at: 1204 1982 2172 2964 1204
Size: 778 190 792 2430
Fragments arranged by size:
2430 792 778 190
Bsp1286I G_dGCh'C
Cuts at: 373 384 466 576 631 769 791 1054 1204
Size: 11 82 110 55 138 22 263 150
Cuts at: 1204 1815 1908 1982 2172 2234 2964 373
Size: 611 93 74 190 62 730 1599
Fragments arranged by size:
1599 730 611 263 190 150 138 110
93 82 74 62 55 22 11
BspEI T'CCGG_A
Cuts at: 3410 3410
Size: 4190
BspHI T'CATG_A
Cuts at: 191 1115 3366 191
Size: 924 2251 1015
Fragments arranged by size:
2251 1015 924
BspMI ACCTGCnnnn'nnnn_
Cuts at: 1240 1454 1756 2137 1240
Size: 214 302 381 3293
Fragments arranged by size:
3293 381 302 214
BsrBI CCG'CTC
Cuts at: 50 482 2482 2536 4020 4185 50
Size: 432 2000 54 1484 165 55
Fragments arranged by size:
2000 1484 432 165 55 54
BsrDI GCAATG_nn'
Cuts at: 348 2102 348
Size: 1754 2436
BsrFI r'CCGG_y
Cuts at: 1169 2188 2369 2578 1169
Size: 1019 181 209 2781
Fragments arranged by size:
2781 1019 209 181
BssHII G'CGCG_C
Cuts at: 611 611
Size: 4190
BssSI C'ACGA_G
Cuts at: 457 2461 2819 457
Size: 2004 358 1828
Fragments arranged by size:
2004 1828 358
BstAPI GCAn_nnn'nTGC
Cuts at: 1567 1567
Size: 4190
BstBI TT'CG_AA
Cuts at: 717 2551 717
Size: 1834 2356
BstEII G'GTnAC_C
Cuts at: 177 177
Size: 4190
BstYI r'GATC_y
Cuts at: 1 1034 1215 1682 1705 2040 2286 3298 3384
Size: 1033 181 467 23 335 246 1012 86
Cuts at: 3384 3396 3742 1
Size: 12 346 449
Fragments arranged by size:
1033 1012 467 449 346 335 246 181
86 23 12
Bsu36I CC'TnA_GG
Cuts at: 159 159
Size: 4190
BtgI C'CryG_G
Cuts at: 107 242 428 599 107
Size: 135 186 171 3698
Fragments arranged by size:
3698 186 171 135
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 988 2140 2321 2484 988
Size: 1152 181 163 2694
Fragments arranged by size:
2694 1152 181 163
BtsI GCAGTG_nn'
Cuts at: 533 731 770 923 1433 533
Size: 198 39 153 510 3290
Fragments arranged by size:
3290 510 198 153 39
DraI TTT'AAA
Cuts at: 1189 1189
Size: 4190
DrdI GACnn_nn'nnGTC
Cuts at: 1896 2754 1896
Size: 858 3332
EaeI y'GGCC_r
Cuts at: 245 1775 1949 2340 2367 3712 245
Size: 1530 174 391 27 1345 723
Fragments arranged by size:
1530 1345 723 391 174 27
EagI C'GGCC_G
Cuts at: 1775 1775
Size: 4190
EarI CTCTTCn'nnn_
Cuts at: 193 823 2213 2423 193
Size: 630 1390 210 1960
Fragments arranged by size:
1960 1390 630 210
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 2398 2708 2854 4000 2398
Size: 310 146 1146 2588
Fragments arranged by size:
2588 1146 310 146
EcoICRI GAG'CTC
Cuts at: 1202 1202
Size: 4190
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 127 252 373 498 571 673 2015 2447 3194
Size: 125 121 125 73 102 1342 432 747
Cuts at: 3194 3462 127
Size: 268 855
Fragments arranged by size:
1342 855 747 432 268 125 125 121
102 73
EcoO109I rG'GnC_Cy
Cuts at: 120 470 765 766 1329 120
Size: 350 295 1 563 2981
Fragments arranged by size:
2981 563 350 295 1
EcoRI G'AATT_C
Cuts at: 1194 1194
Size: 4190
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 1080 2209 1080
Size: 1129 3061
FspI TGC'GCA
Cuts at: 1971 1971
Size: 4190
HaeII r_GCGC'y
Cuts at: 747 1624 1872 2894 3873 747
Size: 877 248 1022 979 1064
Fragments arranged by size:
1064 1022 979 877 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 2032 2062 2064 2094 2032
Size: 30 2 30 4128
Fragments arranged by size:
4128 30 30 2
HincII GTy'rAC
Cuts at: 1229 1229
Size: 4190
HindIII A'AGCT_T
Cuts at: 1143 1143
Size: 4190
KasI G'GCGC_C
Cuts at: 1620 1868 1620
Size: 248 3942
KpnI G_GTAC'C
Cuts at: 1210 1210
Size: 4190
MluI A'CGCG_T
Cuts at: 3623 3623
Size: 4190
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 2861 3045 2861
Size: 184 4006
MscI TGG'CCA
Cuts at: 247 1951 247
Size: 1704 2486
MslI CAynn'nnrTG
Cuts at: 499 2179 2306 499
Size: 1680 127 2383
Fragments arranged by size:
2383 1680 127
MspA1I CmG'CkG
Cuts at: 234 386 1301 1615 1975 2988 3233 234
Size: 152 915 314 360 1013 245 1191
Fragments arranged by size:
1191 1013 915 360 314 245 152
NaeI GCC'GGC
Cuts at: 2371 2371
Size: 4190
NarI GG'CG_CC
Cuts at: 1621 1869 1621
Size: 248 3942
NcoI C'CATG_G
Cuts at: 107 242 428 599 107
Size: 135 186 171 3698
Fragments arranged by size:
3698 186 171 135
NgoMIV G'CCGG_C
Cuts at: 2369 2369
Size: 4190
NspI r_CATG'y
Cuts at: 688 1006 1243 1743 2650 688
Size: 318 237 500 907 2228
Fragments arranged by size:
2228 907 500 318 237
PciI A'CATG_T
Cuts at: 2646 2646
Size: 4190
PflMI CCAn_nnn'nTGG
Cuts at: 150 275 150
Size: 125 4065
PfoI T'CCnGG_A
Cuts at: 350 350
Size: 4190
PmeI GTTT'AAAC
Cuts at: 1189 1189
Size: 4190
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3329 3361 3329
Size: 32 4158
PpuMI rG'GwC_Cy
Cuts at: 120 470 120
Size: 350 3840
PspOMI G'GGCC_C
Cuts at: 765 765
Size: 4190
PstI C_TGCA'G
Cuts at: 227 677 726 918 1237 1922 227
Size: 450 49 192 319 685 2495
Fragments arranged by size:
2495 685 450 319 192 49
PvuI CG_AT'CG
Cuts at: 104 104
Size: 4190
PvuII CAG'CTG
Cuts at: 1615 1975 1615
Size: 360 3830
RsrII CG'GwC_CG
Cuts at: 2385 2385
Size: 4190
SacI G_AGCT'C
Cuts at: 1204 1204
Size: 4190
SalI G'TCGA_C
Cuts at: 1227 1227
Size: 4190
SapI GCTCTTCn'nnn_
Cuts at: 823 2213 2423 823
Size: 1390 210 2590
Fragments arranged by size:
2590 1390 210
SbfI CC_TGCA'GG
Cuts at: 1237 1237
Size: 4190
ScaI AGT'ACT
Cuts at: 3707 3707
Size: 4190
SexAI A'CCwGG_T
Cuts at: 394 394
Size: 4190
SfcI C'TryA_G
Cuts at: 33 223 673 722 914 1233 1918 2911 3102
Size: 190 450 49 192 319 685 993 191
Cuts at: 3102 33
Size: 1121
Fragments arranged by size:
1121 993 685 450 319 192 191 190
49
SfoI GGC'GCC
Cuts at: 1622 1870 1622
Size: 248 3942
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 4190
SmaI CCC'GGG
Cuts at: 1212 1212
Size: 4190
SmlI C'TyrA_G
Cuts at: 1345 2260 2752 3014 1345
Size: 915 492 262 2521
Fragments arranged by size:
2521 915 492 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 4190
SphI G_CATG'C
Cuts at: 1243 1743 1243
Size: 500 3690
StuI AGG'CCT
Cuts at: 556 556
Size: 4190
StyI C'CwwG_G
Cuts at: 107 242 428 599 1055 1324 1678 107
Size: 135 186 171 456 269 354 2619
Fragments arranged by size:
2619 456 354 269 186 171 135
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 612 694 1773 1906 2365 2574 612
Size: 82 1079 133 459 209 2228
Fragments arranged by size:
2228 1079 459 209 133 82
TatI w'GTAC_w
Cuts at: 803 3705 803
Size: 2902 1288
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 3707 3739 3707
Size: 32 4158
Tth111I GACn'n_nGTC
Cuts at: 1987 1987
Size: 4190
XbaI T'CTAG_A
Cuts at: 1221 1221
Size: 4190
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 1685 1685
Size: 4190
XmaI C'CCGG_G
Cuts at: 1210 1210
Size: 4190
XmnI GAAnn'nnTTC
Cuts at: 556 556
Size: 4190
Enzymes that do cut:
AccI Acc65I AclI AcuI AflIII AgeI AleI AlwNI
ApaI ApaLI ApoI AseI AsiSI AvaI BamHI BanI
BanII BbeI BciVI BclI BglII BlpI Bme1580I BmgBI
BmrI BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI
BsaHI BsaWI BsaXI BseRI BseYI BsiEI BsiHKAI Bsp1286I
BspEI BspHI BspMI BsrBI BsrDI BsrFI BssHII BssSI
BstAPI BstBI BstEII BstYI Bsu36I BtgI BtgZI BtsI
DraI DrdI EaeI EagI EarI EciI EcoICRI Eco57MI
EcoO109I EcoRI FalI FspI HaeII Hin4I HincII HindIII
KasI KpnI MluI MmeI MscI MslI MspA1I NaeI
NarI NcoI NgoMIV NspI PciI PflMI PfoI PmeI
PpiI PpuMI PspOMI PstI PvuI PvuII RsrII SacI
SalI SapI SbfI ScaI SexAI SfcI SfoI SgfI
SmaI SmlI SpeI SphI StuI StyI TaqII TatI
TstI Tth111I XbaI XcmI XmaI XmnI
Enzymes that do not cut:
AarI AatII AfeI AflII AhdI AlfI AloI AscI
AvrII BaeI BbsI BbvCI BcgI BfrBI BglI BmtI
BsgI BsiWI BsmI BsmBI BsrGI BstXI BstZ17I ClaI
CspCI DraIII EcoNI EcoRV FseI FspAI HpaI MfeI
NdeI NheI NotI NruI NsiI PacI PasI PmlI
PshAI PsiI PspXI PsrI SacII SanDI SfiI SgrAI
SnaBI SrfI SspI SwaI XhoI ZraI