Restriction map for
pF1KE0104
(Circular) (Six-base) MAPSORT of: pF1KE0104.seq Check: 9733 from: 1 to: 4499
pF1KE0104 4499 bp
With 182 enzymes: SgfI *
April 24, 2009 13:10 ..
AccI GT'mk_AC
Cuts at: 1324 1537 1324
Size: 213 4286
Acc65I G'GTAC_C
Cuts at: 1515 1515
Size: 4499
AclI AA'CG_TT
Cuts at: 4339 4339
Size: 4499
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 151 191 839 2324 2756 3503 3771 151
Size: 40 648 1485 432 747 268 879
Fragments arranged by size:
1485 879 747 648 432 268 40
AflIII A'CryG_T
Cuts at: 1851 2955 3932 1851
Size: 1104 977 2418
Fragments arranged by size:
2418 1104 977
AgeI A'CCGG_T
Cuts at: 2887 2887
Size: 4499
AlwNI CAG_nnn'CTG
Cuts at: 169 1389 1804 3371 169
Size: 1220 415 1567 1297
Fragments arranged by size:
1567 1297 1220 415
ApaLI G'TGCA_C
Cuts at: 3269 3269
Size: 4499
ApoI r'AATT_y
Cuts at: 823 1503 3852 4108 823
Size: 680 2349 256 1214
Fragments arranged by size:
2349 1214 680 256
AseI AT'TA_AT
Cuts at: 20 1459 20
Size: 1439 3060
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 4499
AvaI C'yCGr_G
Cuts at: 756 1519 756
Size: 763 3736
BamHI G'GATC_C
Cuts at: 1524 1524
Size: 4499
BanI G'GyrC_C
Cuts at: 278 1515 1929 2177 2212 278
Size: 1237 414 248 35 2565
Fragments arranged by size:
2565 1237 414 248 35
BanII G_rGCy'C
Cuts at: 1513 2543 1513
Size: 1030 3469
BbeI G_GCGC'C
Cuts at: 1933 2181 1933
Size: 248 4251
BbsI GAAGACnn'nnnn_
Cuts at: 104 1028 104
Size: 924 3575
BciVI GTATCCnnnnn_n'
Cuts at: 2397 3158 4499 2397
Size: 761 1341 2397
Fragments arranged by size:
2397 1341 761
BclI T'GATC_A
Cuts at: 2019 2019
Size: 4499
BglI GCCn_nnn'nGGC
Cuts at: 1124 1124
Size: 4499
BglII A'GATC_T
Cuts at: 922 2014 4051 922
Size: 1092 2037 1370
Fragments arranged by size:
2037 1370 1092
BlpI GC'TnA_GC
Cuts at: 1308 1611 1308
Size: 303 4196
Bme1580I G_kGCm'C
Cuts at: 281 2124 2217 3273 281
Size: 1843 93 1056 1507
Fragments arranged by size:
1843 1507 1056 93
BmrI ACTGGGnnnn_n'
Cuts at: 1711 1792 2127 4271 1711
Size: 81 335 2144 1939
Fragments arranged by size:
2144 1939 335 81
BmtI G_CTAG'C
Cuts at: 529 529
Size: 4499
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2866 2866
Size: 4499
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1205 1205
Size: 4499
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 325 899 1675 2554 3046 3344 325
Size: 574 776 879 492 298 1480
Fragments arranged by size:
1480 879 776 574 492 298
BsaI GGTCTCn'nnnn_
Cuts at: 4203 4203
Size: 4499
BsaAI yAC'GTr
Cuts at: 1852 1852
Size: 4499
BsaBI GATnn'nnATC
Cuts at: 6 575 2013 4050 6
Size: 569 1438 2037 455
Fragments arranged by size:
2037 1438 569 455
BsaHI Gr'CG_yC
Cuts at: 1043 1930 2178 2880 4376 1043
Size: 887 248 702 1496 1166
Fragments arranged by size:
1496 1166 887 702 248
BsaWI w'CCGG_w
Cuts at: 488 1688 2209 2887 3161 3308 3719 488
Size: 1200 521 678 274 147 411 1268
Fragments arranged by size:
1268 1200 678 521 411 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1226 1256 1226
Size: 30 4469
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 778 778
Size: 4499
BseYI C'CCAG_C
Cuts at: 674 901 1924 2682 3259 3755 674
Size: 227 1023 758 577 496 1418
Fragments arranged by size:
1418 1023 758 577 496 227
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 335 335
Size: 4499
BsiEI CG_ry'CG
Cuts at: 104 2041 2087 3295 104
Size: 1937 46 1208 1308
Fragments arranged by size:
1937 1308 1208 46
BsiHKAI G_wGCw'C
Cuts at: 1513 2291 2481 3273 1513
Size: 778 190 792 2739
Fragments arranged by size:
2739 792 778 190
BsmI GAATG_Cn'
Cuts at: 1420 1420
Size: 4499
Bsp1286I G_dGCh'C
Cuts at: 281 1513 2124 2217 2291 2481 2543 3273 281
Size: 1232 611 93 74 190 62 730 1507
Fragments arranged by size:
1507 1232 730 611 190 93 74 62
BspEI T'CCGG_A
Cuts at: 3719 3719
Size: 4499
BspHI T'CATG_A
Cuts at: 3675 3675
Size: 4499
BspMI ACCTGCnnnn'nnnn_
Cuts at: 1549 1763 2065 2446 1549
Size: 214 302 381 3602
Fragments arranged by size:
3602 381 302 214
BsrBI CCG'CTC
Cuts at: 50 2791 2845 4329 4494 50
Size: 2741 54 1484 165 55
Fragments arranged by size:
2741 1484 165 55 54
BsrDI GCAATG_nn'
Cuts at: 2411 2411
Size: 4499
BsrFI r'CCGG_y
Cuts at: 2497 2678 2887 2497
Size: 181 209 4109
Fragments arranged by size:
4109 209 181
BssSI C'ACGA_G
Cuts at: 2770 3128 2770
Size: 358 4141
BstAPI GCAn_nnn'nTGC
Cuts at: 1876 1876
Size: 4499
BstBI TT'CG_AA
Cuts at: 2860 2860
Size: 4499
BstYI r'GATC_y
Cuts at: 1 804 922 1524 1991 2014 2349 2595 3607
Size: 803 118 602 467 23 335 246 1012
Cuts at: 3607 3693 3705 4051 1
Size: 86 12 346 449
Fragments arranged by size:
1012 803 602 467 449 346 335 246
118 86 23 12
BtgI C'CryG_G
Cuts at: 1273 1273
Size: 4499
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 2449 2630 2793 2449
Size: 181 163 4155
Fragments arranged by size:
4155 181 163
BtsI GCAGTG_nn'
Cuts at: 1325 1742 1325
Size: 417 4082
DraI TTT'AAA
Cuts at: 575 1016 1498 575
Size: 441 482 3576
Fragments arranged by size:
3576 482 441
DrdI GACnn_nn'nnGTC
Cuts at: 2205 3063 2205
Size: 858 3641
EaeI y'GGCC_r
Cuts at: 1245 1276 2084 2258 2649 2676 4021 1245
Size: 31 808 174 391 27 1345 1723
Fragments arranged by size:
1723 1345 808 391 174 31 27
EagI C'GGCC_G
Cuts at: 2084 2084
Size: 4499
EarI CTCTTCn'nnn_
Cuts at: 224 1217 2522 2732 224
Size: 993 1305 210 1991
Fragments arranged by size:
1991 1305 993 210
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 2707 3017 3163 4309 2707
Size: 310 146 1146 2897
Fragments arranged by size:
2897 1146 310 146
EcoICRI GAG'CTC
Cuts at: 1511 1511
Size: 4499
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 151 191 839 1205 2324 2756 3503 3771 151
Size: 40 648 366 1119 432 747 268 879
Fragments arranged by size:
1119 879 747 648 432 366 268 40
EcoO109I rG'GnC_Cy
Cuts at: 1386 1638 1386
Size: 252 4247
EcoRI G'AATT_C
Cuts at: 823 1503 823
Size: 680 3819
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 649 708 2518 649
Size: 59 1810 2630
Fragments arranged by size:
2630 1810 59
FspI TGC'GCA
Cuts at: 2280 2280
Size: 4499
HaeII r_GCGC'y
Cuts at: 1933 2181 3203 4182 1933
Size: 248 1022 979 2250
Fragments arranged by size:
2250 1022 979 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 314 346 1370 1402 2341 2371 2373 2403 314
Size: 32 1024 32 939 30 2 30 2410
Fragments arranged by size:
2410 1024 939 32 32 30 30 2
HincII GTy'rAC
Cuts at: 191 1270 1341 1538 191
Size: 1079 71 197 3152
Fragments arranged by size:
3152 1079 197 71
HpaI GTT'AAC
Cuts at: 191 191
Size: 4499
KasI G'GCGC_C
Cuts at: 1929 2177 1929
Size: 248 4251
KpnI G_GTAC'C
Cuts at: 1519 1519
Size: 4499
MfeI C'AATT_G
Cuts at: 326 883 326
Size: 557 3942
MluI A'CGCG_T
Cuts at: 3932 3932
Size: 4499
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 639 3170 3354 639
Size: 2531 184 1784
Fragments arranged by size:
2531 1784 184
MscI TGG'CCA
Cuts at: 1247 1278 2260 1247
Size: 31 982 3486
Fragments arranged by size:
3486 982 31
MslI CAynn'nnrTG
Cuts at: 1103 2488 2615 1103
Size: 1385 127 2987
Fragments arranged by size:
2987 1385 127
MspA1I CmG'CkG
Cuts at: 166 642 1367 1610 1924 2284 3297 3542 166
Size: 476 725 243 314 360 1013 245 1123
Fragments arranged by size:
1123 1013 725 476 360 314 245 243
NaeI GCC'GGC
Cuts at: 2680 2680
Size: 4499
NarI GG'CG_CC
Cuts at: 1930 2178 1930
Size: 248 4251
NcoI C'CATG_G
Cuts at: 1273 1273
Size: 4499
NgoMIV G'CCGG_C
Cuts at: 2678 2678
Size: 4499
NheI G'CTAG_C
Cuts at: 525 525
Size: 4499
NspI r_CATG'y
Cuts at: 1155 1552 2052 2959 1155
Size: 397 500 907 2695
Fragments arranged by size:
2695 907 500 397
PciI A'CATG_T
Cuts at: 2955 2955
Size: 4499
PflMI CCAn_nnn'nTGG
Cuts at: 967 967
Size: 4499
PfoI T'CCnGG_A
Cuts at: 1388 1388
Size: 4499
PmeI GTTT'AAAC
Cuts at: 1498 1498
Size: 4499
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3638 3670 3638
Size: 32 4467
PpuMI rG'GwC_Cy
Cuts at: 1386 1386
Size: 4499
PsiI TTA'TAA
Cuts at: 380 380
Size: 4499
PstI C_TGCA'G
Cuts at: 1546 2231 1546
Size: 685 3814
PvuI CG_AT'CG
Cuts at: 104 104
Size: 4499
PvuII CAG'CTG
Cuts at: 166 642 1924 2284 166
Size: 476 1282 360 2381
Fragments arranged by size:
2381 1282 476 360
RsrII CG'GwC_CG
Cuts at: 2694 2694
Size: 4499
SacI G_AGCT'C
Cuts at: 1513 1513
Size: 4499
SalI G'TCGA_C
Cuts at: 1536 1536
Size: 4499
SapI GCTCTTCn'nnn_
Cuts at: 2522 2732 2522
Size: 210 4289
SbfI CC_TGCA'GG
Cuts at: 1546 1546
Size: 4499
ScaI AGT'ACT
Cuts at: 4016 4016
Size: 4499
SfcI C'TryA_G
Cuts at: 33 1325 1542 2227 3220 3411 33
Size: 1292 217 685 993 191 1121
Fragments arranged by size:
1292 1121 993 685 217 191
SfoI GGC'GCC
Cuts at: 1931 2179 1931
Size: 248 4251
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 4499
SmaI CCC'GGG
Cuts at: 1521 1521
Size: 4499
SmlI C'TyrA_G
Cuts at: 304 914 1654 2569 3061 3323 304
Size: 610 740 915 492 262 1480
Fragments arranged by size:
1480 915 740 610 492 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 4499
SphI G_CATG'C
Cuts at: 1552 2052 1552
Size: 500 3999
StyI C'CwwG_G
Cuts at: 427 581 1273 1633 1987 427
Size: 154 692 360 354 2939
Fragments arranged by size:
2939 692 360 354 154
SwaI ATTT'AAAT
Cuts at: 575 575
Size: 4499
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 296 2082 2215 2674 2883 296
Size: 1786 133 459 209 1912
Fragments arranged by size:
1912 1786 459 209 133
TatI w'GTAC_w
Cuts at: 4014 4014
Size: 4499
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 4016 4048 4016
Size: 32 4467
Tth111I GACn'n_nGTC
Cuts at: 2296 2296
Size: 4499
XbaI T'CTAG_A
Cuts at: 1530 1530
Size: 4499
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 1994 1994
Size: 4499
XmaI C'CCGG_G
Cuts at: 1519 1519
Size: 4499
Enzymes that do cut:
AccI Acc65I AclI AcuI AflIII AgeI AlwNI ApaLI
ApoI AseI AsiSI AvaI BamHI BanI BanII BbeI
BbsI BciVI BclI BglI BglII BlpI Bme1580I BmrI
BmtI BplI BpmI BpuEI BsaI BsaAI BsaBI BsaHI
BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI
Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BssSI
BstAPI BstBI BstYI BtgI BtgZI BtsI DraI DrdI
EaeI EagI EarI EciI EcoICRI Eco57MI EcoO109I EcoRI
FalI FspI HaeII Hin4I HincII HpaI KasI KpnI
MfeI MluI MmeI MscI MslI MspA1I NaeI NarI
NcoI NgoMIV NheI NspI PciI PflMI PfoI PmeI
PpiI PpuMI PsiI PstI PvuI PvuII RsrII SacI
SalI SapI SbfI ScaI SfcI SfoI SgfI SmaI
SmlI SpeI SphI StyI SwaI TaqII TatI TstI
Tth111I XbaI XcmI XmaI
Enzymes that do not cut:
AarI AatII AfeI AflII AhdI AleI AlfI AloI
ApaI AscI AvrII BaeI BbvCI BcgI BfrBI BmgBI
Bpu10I BsiWI BsmBI BsrGI BssHII BstEII BstXI BstZ17I
Bsu36I ClaI CspCI DraIII EcoNI EcoRV FseI FspAI
HindIII NdeI NotI NruI NsiI PacI PasI PmlI
PshAI PspOMI PspXI PsrI SacII SanDI SexAI SfiI
SgrAI SnaBI SrfI SspI StuI XhoI XmnI ZraI