Restriction map for
pF1KE0106
(Circular) (Six-base) MAPSORT of: pF1KE0106.seq Check: 711 from: 1 to: 3956
pF1KE0106 3956 bp
With 182 enzymes: SgfI *
April 24, 2009 13:10 ..
AccI GT'mk_AC
Cuts at: 994 994
Size: 3956
Acc65I G'GTAC_C
Cuts at: 972 972
Size: 3956
AclI AA'CG_TT
Cuts at: 3796 3796
Size: 3956
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 487 1781 2213 2960 3228 487
Size: 1294 432 747 268 1215
Fragments arranged by size:
1294 1215 747 432 268
AflIII A'CryG_T
Cuts at: 1308 2412 3389 1308
Size: 1104 977 1875
Fragments arranged by size:
1875 1104 977
AgeI A'CCGG_T
Cuts at: 2344 2344
Size: 3956
AlwNI CAG_nnn'CTG
Cuts at: 233 381 531 846 1261 2828 233
Size: 148 150 315 415 1567 1361
Fragments arranged by size:
1567 1361 415 315 150 148
ApaI G_GGCC'C
Cuts at: 737 737
Size: 3956
ApaLI G'TGCA_C
Cuts at: 2726 2726
Size: 3956
ApoI r'AATT_y
Cuts at: 823 960 3309 3565 823
Size: 137 2349 256 1214
Fragments arranged by size:
2349 1214 256 137
AseI AT'TA_AT
Cuts at: 20 20
Size: 3956
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 3956
AvaI C'yCGr_G
Cuts at: 976 976
Size: 3956
BamHI G'GATC_C
Cuts at: 981 981
Size: 3956
BanI G'GyrC_C
Cuts at: 433 640 972 1386 1634 1669 433
Size: 207 332 414 248 35 2720
Fragments arranged by size:
2720 414 332 248 207 35
BanII G_rGCy'C
Cuts at: 339 737 970 2000 339
Size: 398 233 1030 2295
Fragments arranged by size:
2295 1030 398 233
BbeI G_GCGC'C
Cuts at: 1390 1638 1390
Size: 248 3708
BbsI GAAGACnn'nnnn_
Cuts at: 565 565
Size: 3956
BbvCI CC'TCA_GC
Cuts at: 711 711
Size: 3956
BciVI GTATCCnnnnn_n'
Cuts at: 1854 2615 3956 1854
Size: 761 1341 1854
Fragments arranged by size:
1854 1341 761
BclI T'GATC_A
Cuts at: 1476 1476
Size: 3956
BglII A'GATC_T
Cuts at: 1471 3508 1471
Size: 2037 1919
BlpI GC'TnA_GC
Cuts at: 1068 1068
Size: 3956
Bme1580I G_kGCm'C
Cuts at: 737 1581 1674 2730 737
Size: 844 93 1056 1963
Fragments arranged by size:
1963 1056 844 93
BmrI ACTGGGnnnn_n'
Cuts at: 369 1168 1249 1584 3728 369
Size: 799 81 335 2144 597
Fragments arranged by size:
2144 799 597 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2323 2323
Size: 3956
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 230 895 230
Size: 665 3291
Bpu10I CC'TnA_GC
Cuts at: 343 711 343
Size: 368 3588
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 406 841 1132 2011 2503 2801 406
Size: 435 291 879 492 298 1561
Fragments arranged by size:
1561 879 492 435 298 291
BsaI GGTCTCn'nnnn_
Cuts at: 135 323 3660 135
Size: 188 3337 431
Fragments arranged by size:
3337 431 188
BsaAI yAC'GTr
Cuts at: 1309 1309
Size: 3956
BsaBI GATnn'nnATC
Cuts at: 6 1470 3507 6
Size: 1464 2037 455
Fragments arranged by size:
2037 1464 455
BsaHI Gr'CG_yC
Cuts at: 214 474 1387 1635 2337 3833 214
Size: 260 913 248 702 1496 337
Fragments arranged by size:
1496 913 702 337 260 248
BsaWI w'CCGG_w
Cuts at: 1145 1666 2344 2618 2765 3176 1145
Size: 521 678 274 147 411 1925
Fragments arranged by size:
1925 678 521 411 274 147
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 233 233
Size: 3956
BseYI C'CCAG_C
Cuts at: 715 768 1381 2139 2716 3212 715
Size: 53 613 758 577 496 1459
Fragments arranged by size:
1459 758 613 577 496 53
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 367 367
Size: 3956
BsiEI CG_ry'CG
Cuts at: 104 398 1498 1544 2752 104
Size: 294 1100 46 1208 1308
Fragments arranged by size:
1308 1208 1100 294 46
BsiHKAI G_wGCw'C
Cuts at: 265 793 881 970 1748 1938 2730 265
Size: 528 88 89 778 190 792 1491
Fragments arranged by size:
1491 792 778 528 190 89 88
Bsp1286I G_dGCh'C
Cuts at: 265 339 737 793 881 970 1581 1674 1748
Size: 74 398 56 88 89 611 93 74
Cuts at: 1748 1938 2000 2730 265
Size: 190 62 730 1491
Fragments arranged by size:
1491 730 611 398 190 93 89 88
74 74 62 56
BspEI T'CCGG_A
Cuts at: 3176 3176
Size: 3956
BspHI T'CATG_A
Cuts at: 3132 3132
Size: 3956
BspMI ACCTGCnnnn'nnnn_
Cuts at: 410 1006 1220 1522 1903 410
Size: 596 214 302 381 2463
Fragments arranged by size:
2463 596 381 302 214
BsrBI CCG'CTC
Cuts at: 50 2248 2302 3786 3951 50
Size: 2198 54 1484 165 55
Fragments arranged by size:
2198 1484 165 55 54
BsrDI GCAATG_nn'
Cuts at: 1868 1868
Size: 3956
BsrFI r'CCGG_y
Cuts at: 356 1954 2135 2344 356
Size: 1598 181 209 1968
Fragments arranged by size:
1968 1598 209 181
BssSI C'ACGA_G
Cuts at: 2227 2585 2227
Size: 358 3598
BstAPI GCAn_nnn'nTGC
Cuts at: 846 885 1333 846
Size: 39 448 3469
Fragments arranged by size:
3469 448 39
BstBI TT'CG_AA
Cuts at: 2317 2317
Size: 3956
BstXI CCAn_nnnn'nTGG
Cuts at: 943 943
Size: 3956
BstYI r'GATC_y
Cuts at: 1 656 981 1448 1471 1806 2052 3064 3150
Size: 655 325 467 23 335 246 1012 86
Cuts at: 3150 3162 3508 1
Size: 12 346 449
Fragments arranged by size:
1012 655 467 449 346 335 325 246
86 23 12
BtgI C'CryG_G
Cuts at: 136 136
Size: 3956
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 1906 2087 2250 1906
Size: 181 163 3612
Fragments arranged by size:
3612 181 163
BtsI GCAGTG_nn'
Cuts at: 465 1199 465
Size: 734 3222
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 728 763 728
Size: 35 3921
DraI TTT'AAA
Cuts at: 955 955
Size: 3956
DraIII CAC_nnn'GTG
Cuts at: 885 885
Size: 3956
DrdI GACnn_nn'nnGTC
Cuts at: 1662 2520 1662
Size: 858 3098
EaeI y'GGCC_r
Cuts at: 395 540 626 945 1541 1715 2106 2133 3478
Size: 145 86 319 596 174 391 27 1345
Cuts at: 3478 395
Size: 873
Fragments arranged by size:
1345 873 596 391 319 174 145 86
27
EagI C'GGCC_G
Cuts at: 395 1541 395
Size: 1146 2810
EarI CTCTTCn'nnn_
Cuts at: 590 1979 2189 590
Size: 1389 210 2357
Fragments arranged by size:
2357 1389 210
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 2164 2474 2620 3766 2164
Size: 310 146 1146 2354
Fragments arranged by size:
2354 1146 310 146
EcoICRI GAG'CTC
Cuts at: 968 968
Size: 3956
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 230 487 895 1781 2213 2960 3228 230
Size: 257 408 886 432 747 268 958
Fragments arranged by size:
958 886 747 432 408 268 257
EcoNI CCTnn'n_nnAGG
Cuts at: 248 385 481 248
Size: 137 96 3723
Fragments arranged by size:
3723 137 96
EcoO109I rG'GnC_Cy
Cuts at: 528 699 720 734 1095 528
Size: 171 21 14 361 3389
Fragments arranged by size:
3389 361 171 21 14
EcoRI G'AATT_C
Cuts at: 823 960 823
Size: 137 3819
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 1975 1975
Size: 3956
FspI TGC'GCA
Cuts at: 1737 1737
Size: 3956
HaeII r_GCGC'y
Cuts at: 357 1390 1638 2660 3639 357
Size: 1033 248 1022 979 674
Fragments arranged by size:
1033 1022 979 674 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 567 599 1798 1828 1830 1860 567
Size: 32 1199 30 2 30 2663
Fragments arranged by size:
2663 1199 32 30 30 2
HincII GTy'rAC
Cuts at: 594 633 995 594
Size: 39 362 3555
Fragments arranged by size:
3555 362 39
KasI G'GCGC_C
Cuts at: 1386 1634 1386
Size: 248 3708
KpnI G_GTAC'C
Cuts at: 976 976
Size: 3956
MluI A'CGCG_T
Cuts at: 3389 3389
Size: 3956
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 133 737 2627 2811 133
Size: 604 1890 184 1278
Fragments arranged by size:
1890 1278 604 184
MscI TGG'CCA
Cuts at: 542 947 1717 542
Size: 405 770 2781
Fragments arranged by size:
2781 770 405
MslI CAynn'nnrTG
Cuts at: 1945 2072 1945
Size: 127 3829
MspA1I CmG'CkG
Cuts at: 411 615 715 768 1067 1381 1741 2754 2999
Size: 204 100 53 299 314 360 1013 245
Cuts at: 2999 411
Size: 1368
Fragments arranged by size:
1368 1013 360 314 299 245 204 100
53
NaeI GCC'GGC
Cuts at: 358 2137 358
Size: 1779 2177
NarI GG'CG_CC
Cuts at: 1387 1635 1387
Size: 248 3708
NgoMIV G'CCGG_C
Cuts at: 356 2135 356
Size: 1779 2177
NspI r_CATG'y
Cuts at: 1009 1509 2416 1009
Size: 500 907 2549
Fragments arranged by size:
2549 907 500
PasI CC'CwG_GG
Cuts at: 730 730
Size: 3956
PciI A'CATG_T
Cuts at: 2412 2412
Size: 3956
PmeI GTTT'AAAC
Cuts at: 955 955
Size: 3956
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3095 3127 3095
Size: 32 3924
PpuMI rG'GwC_Cy
Cuts at: 528 699 720 528
Size: 171 21 3764
Fragments arranged by size:
3764 171 21
PspOMI G'GGCC_C
Cuts at: 733 733
Size: 3956
PstI C_TGCA'G
Cuts at: 169 842 1003 1688 169
Size: 673 161 685 2437
Fragments arranged by size:
2437 685 673 161
PvuI CG_AT'CG
Cuts at: 104 104
Size: 3956
PvuII CAG'CTG
Cuts at: 615 715 768 1381 1741 615
Size: 100 53 613 360 2830
Fragments arranged by size:
2830 613 360 100 53
RsrII CG'GwC_CG
Cuts at: 2151 2151
Size: 3956
SacI G_AGCT'C
Cuts at: 970 970
Size: 3956
SalI G'TCGA_C
Cuts at: 993 993
Size: 3956
SapI GCTCTTCn'nnn_
Cuts at: 590 1979 2189 590
Size: 1389 210 2357
Fragments arranged by size:
2357 1389 210
SbfI CC_TGCA'GG
Cuts at: 1003 1003
Size: 3956
ScaI AGT'ACT
Cuts at: 3473 3473
Size: 3956
SfcI C'TryA_G
Cuts at: 33 165 763 838 999 1684 2677 2868 33
Size: 132 598 75 161 685 993 191 1121
Fragments arranged by size:
1121 993 685 598 191 161 132 75
SfoI GGC'GCC
Cuts at: 1388 1636 1388
Size: 248 3708
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 3956
SmaI CCC'GGG
Cuts at: 978 978
Size: 3956
SmlI C'TyrA_G
Cuts at: 421 856 1111 2026 2518 2780 421
Size: 435 255 915 492 262 1597
Fragments arranged by size:
1597 915 492 435 262 255
SpeI A'CTAG_T
Cuts at: 67 67
Size: 3956
SphI G_CATG'C
Cuts at: 1009 1509 1009
Size: 500 3456
StyI C'CwwG_G
Cuts at: 440 1090 1444 440
Size: 650 354 2952
Fragments arranged by size:
2952 650 354
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 347 388 658 1539 1672 2131 2340 347
Size: 41 270 881 133 459 209 1963
Fragments arranged by size:
1963 881 459 270 209 133 41
TatI w'GTAC_w
Cuts at: 3471 3471
Size: 3956
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 3473 3505 3473
Size: 32 3924
Tth111I GACn'n_nGTC
Cuts at: 1753 1753
Size: 3956
XbaI T'CTAG_A
Cuts at: 987 987
Size: 3956
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 1451 1451
Size: 3956
XmaI C'CCGG_G
Cuts at: 976 976
Size: 3956
Enzymes that do cut:
AccI Acc65I AclI AcuI AflIII AgeI AlwNI ApaI
ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII
BbeI BbsI BbvCI BciVI BclI BglII BlpI Bme1580I
BmrI BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI
BsaHI BsaWI BseRI BseYI BsgI BsiEI BsiHKAI Bsp1286I
BspEI BspHI BspMI BsrBI BsrDI BsrFI BssSI BstAPI
BstBI BstXI BstYI BtgI BtgZI BtsI CspCI DraI
DraIII DrdI EaeI EagI EarI EciI EcoICRI Eco57MI
EcoNI EcoO109I EcoRI FalI FspI HaeII Hin4I HincII
KasI KpnI MluI MmeI MscI MslI MspA1I NaeI
NarI NgoMIV NspI PasI PciI PmeI PpiI PpuMI
PspOMI PstI PvuI PvuII RsrII SacI SalI SapI
SbfI ScaI SfcI SfoI SgfI SmaI SmlI SpeI
SphI StyI TaqII TatI TstI Tth111I XbaI XcmI
XmaI
Enzymes that do not cut:
AarI AatII AfeI AflII AhdI AleI AlfI AloI
AscI AvrII BaeI BcgI BfrBI BglI BmgBI BmtI
BsaXI BsiWI BsmI BsmBI BsrGI BssHII BstEII BstZ17I
Bsu36I ClaI EcoRV FseI FspAI HindIII HpaI MfeI
NcoI NdeI NheI NotI NruI NsiI PacI PflMI
PfoI PmlI PshAI PsiI PspXI PsrI SacII SanDI
SexAI SfiI SgrAI SnaBI SrfI SspI StuI SwaI
XhoI XmnI ZraI