Restriction map for
pF1KE0109
(Circular) (Six-base) MAPSORT of: pF1KE0109.seq Check: 7143 from: 1 to: 3521
pF1KE0109 3521 bp
With 182 enzymes: SgfI *
April 24, 2009 13:10 ..
AccI GT'mk_AC
Cuts at: 559 559
Size: 3521
Acc65I G'GTAC_C
Cuts at: 537 537
Size: 3521
AclI AA'CG_TT
Cuts at: 3361 3361
Size: 3521
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1346 1778 2525 2793 1346
Size: 432 747 268 2074
Fragments arranged by size:
2074 747 432 268
AflIII A'CryG_T
Cuts at: 873 1977 2954 873
Size: 1104 977 1440
Fragments arranged by size:
1440 1104 977
AgeI A'CCGG_T
Cuts at: 1909 1909
Size: 3521
AlwNI CAG_nnn'CTG
Cuts at: 358 826 2393 358
Size: 468 1567 1486
Fragments arranged by size:
1567 1486 468
ApaLI G'TGCA_C
Cuts at: 2291 2291
Size: 3521
ApoI r'AATT_y
Cuts at: 210 525 2874 3130 210
Size: 315 2349 256 601
Fragments arranged by size:
2349 601 315 256
AseI AT'TA_AT
Cuts at: 20 20
Size: 3521
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 3521
AvaI C'yCGr_G
Cuts at: 287 541 287
Size: 254 3267
BamHI G'GATC_C
Cuts at: 546 546
Size: 3521
BanI G'GyrC_C
Cuts at: 537 951 1199 1234 537
Size: 414 248 35 2824
Fragments arranged by size:
2824 414 248 35
BanII G_rGCy'C
Cuts at: 535 1565 535
Size: 1030 2491
BbeI G_GCGC'C
Cuts at: 955 1203 955
Size: 248 3273
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 90 124 90
Size: 34 3487
BciVI GTATCCnnnnn_n'
Cuts at: 1419 2180 3521 1419
Size: 761 1341 1419
Fragments arranged by size:
1419 1341 761
BclI T'GATC_A
Cuts at: 386 1041 386
Size: 655 2866
BglII A'GATC_T
Cuts at: 1036 3073 1036
Size: 2037 1484
BlpI GC'TnA_GC
Cuts at: 633 633
Size: 3521
Bme1580I G_kGCm'C
Cuts at: 1146 1239 2295 1146
Size: 93 1056 2372
Fragments arranged by size:
2372 1056 93
BmrI ACTGGGnnnn_n'
Cuts at: 199 733 814 1149 3293 199
Size: 534 81 335 2144 427
Fragments arranged by size:
2144 534 427 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 1888 1888
Size: 3521
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 122 232 122
Size: 110 3411
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 400 697 1576 2068 2366 400
Size: 297 879 492 298 1555
Fragments arranged by size:
1555 879 492 298 297
BsaI GGTCTCn'nnnn_
Cuts at: 3225 3225
Size: 3521
BsaAI yAC'GTr
Cuts at: 874 874
Size: 3521
BsaBI GATnn'nnATC
Cuts at: 6 1035 3072 6
Size: 1029 2037 455
Fragments arranged by size:
2037 1029 455
BsaHI Gr'CG_yC
Cuts at: 952 1200 1902 3398 952
Size: 248 702 1496 1075
Fragments arranged by size:
1496 1075 702 248
BsaWI w'CCGG_w
Cuts at: 710 1231 1909 2183 2330 2741 710
Size: 521 678 274 147 411 1490
Fragments arranged by size:
1490 678 521 411 274 147
BseYI C'CCAG_C
Cuts at: 322 946 1704 2281 2777 322
Size: 624 758 577 496 1066
Fragments arranged by size:
1066 758 624 577 496
BsiEI CG_ry'CG
Cuts at: 104 1063 1109 2317 104
Size: 959 46 1208 1308
Fragments arranged by size:
1308 1208 959 46
BsiHKAI G_wGCw'C
Cuts at: 535 1313 1503 2295 535
Size: 778 190 792 1761
Fragments arranged by size:
1761 792 778 190
BsmBI CGTCTCn'nnnn_
Cuts at: 416 416
Size: 3521
Bsp1286I G_dGCh'C
Cuts at: 535 1146 1239 1313 1503 1565 2295 535
Size: 611 93 74 190 62 730 1761
Fragments arranged by size:
1761 730 611 190 93 74 62
BspEI T'CCGG_A
Cuts at: 2741 2741
Size: 3521
BspHI T'CATG_A
Cuts at: 2697 2697
Size: 3521
BspMI ACCTGCnnnn'nnnn_
Cuts at: 571 785 1087 1468 571
Size: 214 302 381 2624
Fragments arranged by size:
2624 381 302 214
BsrBI CCG'CTC
Cuts at: 50 1813 1867 3351 3516 50
Size: 1763 54 1484 165 55
Fragments arranged by size:
1763 1484 165 55 54
BsrDI GCAATG_nn'
Cuts at: 1433 1433
Size: 3521
BsrFI r'CCGG_y
Cuts at: 1519 1700 1909 1519
Size: 181 209 3131
Fragments arranged by size:
3131 209 181
BssSI C'ACGA_G
Cuts at: 1792 2150 1792
Size: 358 3163
BstAPI GCAn_nnn'nTGC
Cuts at: 898 898
Size: 3521
BstBI TT'CG_AA
Cuts at: 1882 1882
Size: 3521
BstYI r'GATC_y
Cuts at: 1 546 1013 1036 1371 1617 2629 2715 2727
Size: 545 467 23 335 246 1012 86 12
Cuts at: 2727 3073 1
Size: 346 449
Fragments arranged by size:
1012 545 467 449 346 335 246 86
23 12
BtgI C'CryG_G
Cuts at: 467 512 467
Size: 45 3476
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 1471 1652 1815 1471
Size: 181 163 3177
Fragments arranged by size:
3177 181 163
BtsI GCAGTG_nn'
Cuts at: 764 764
Size: 3521
DraI TTT'AAA
Cuts at: 520 520
Size: 3521
DrdI GACnn_nn'nnGTC
Cuts at: 1227 2085 1227
Size: 858 2663
EaeI y'GGCC_r
Cuts at: 254 1106 1280 1671 1698 3043 254
Size: 852 174 391 27 1345 732
Fragments arranged by size:
1345 852 732 391 174 27
EagI C'GGCC_G
Cuts at: 1106 1106
Size: 3521
EarI CTCTTCn'nnn_
Cuts at: 433 511 1544 1754 433
Size: 78 1033 210 2200
Fragments arranged by size:
2200 1033 210 78
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 1729 2039 2185 3331 1729
Size: 310 146 1146 1919
Fragments arranged by size:
1919 1146 310 146
EcoICRI GAG'CTC
Cuts at: 533 533
Size: 3521
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 122 232 1346 1778 2525 2793 122
Size: 110 1114 432 747 268 850
Fragments arranged by size:
1114 850 747 432 268 110
EcoNI CCTnn'n_nnAGG
Cuts at: 460 460
Size: 3521
EcoO109I rG'GnC_Cy
Cuts at: 660 660
Size: 3521
EcoRI G'AATT_C
Cuts at: 210 525 210
Size: 315 3206
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 1540 1540
Size: 3521
FspI TGC'GCA
Cuts at: 1302 1302
Size: 3521
HaeII r_GCGC'y
Cuts at: 955 1203 2225 3204 955
Size: 248 1022 979 1272
Fragments arranged by size:
1272 1022 979 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1363 1393 1395 1425 1363
Size: 30 2 30 3459
Fragments arranged by size:
3459 30 30 2
HincII GTy'rAC
Cuts at: 560 560
Size: 3521
KasI G'GCGC_C
Cuts at: 951 1199 951
Size: 248 3273
KpnI G_GTAC'C
Cuts at: 541 541
Size: 3521
MluI A'CGCG_T
Cuts at: 2954 2954
Size: 3521
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 2192 2376 2192
Size: 184 3337
MscI TGG'CCA
Cuts at: 256 1282 256
Size: 1026 2495
MslI CAynn'nnrTG
Cuts at: 1510 1637 1510
Size: 127 3394
MspA1I CmG'CkG
Cuts at: 152 632 946 1306 2319 2564 152
Size: 480 314 360 1013 245 1109
Fragments arranged by size:
1109 1013 480 360 314 245
NaeI GCC'GGC
Cuts at: 1702 1702
Size: 3521
NarI GG'CG_CC
Cuts at: 952 1200 952
Size: 248 3273
NcoI C'CATG_G
Cuts at: 467 512 467
Size: 45 3476
NgoMIV G'CCGG_C
Cuts at: 1700 1700
Size: 3521
NspI r_CATG'y
Cuts at: 574 1074 1981 574
Size: 500 907 2114
Fragments arranged by size:
2114 907 500
PciI A'CATG_T
Cuts at: 1977 1977
Size: 3521
PmeI GTTT'AAAC
Cuts at: 520 520
Size: 3521
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2660 2692 2660
Size: 32 3489
PstI C_TGCA'G
Cuts at: 568 1253 568
Size: 685 2836
PvuI CG_AT'CG
Cuts at: 104 104
Size: 3521
PvuII CAG'CTG
Cuts at: 946 1306 946
Size: 360 3161
RsrII CG'GwC_CG
Cuts at: 1716 1716
Size: 3521
SacI G_AGCT'C
Cuts at: 535 535
Size: 3521
SalI G'TCGA_C
Cuts at: 558 558
Size: 3521
SapI GCTCTTCn'nnn_
Cuts at: 433 1544 1754 433
Size: 1111 210 2200
Fragments arranged by size:
2200 1111 210
SbfI CC_TGCA'GG
Cuts at: 568 568
Size: 3521
ScaI AGT'ACT
Cuts at: 3038 3038
Size: 3521
SfcI C'TryA_G
Cuts at: 33 231 564 1249 2242 2433 33
Size: 198 333 685 993 191 1121
Fragments arranged by size:
1121 993 685 333 198 191
SfoI GGC'GCC
Cuts at: 953 1201 953
Size: 248 3273
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 3521
SmaI CCC'GGG
Cuts at: 289 543 289
Size: 254 3267
SmlI C'TyrA_G
Cuts at: 415 676 1591 2083 2345 415
Size: 261 915 492 262 1591
Fragments arranged by size:
1591 915 492 262 261
SpeI A'CTAG_T
Cuts at: 67 67
Size: 3521
SphI G_CATG'C
Cuts at: 574 1074 574
Size: 500 3021
StuI AGG'CCT
Cuts at: 177 177
Size: 3521
StyI C'CwwG_G
Cuts at: 467 512 655 1009 467
Size: 45 143 354 2979
Fragments arranged by size:
2979 354 143 45
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 1104 1237 1696 1905 1104
Size: 133 459 209 2720
Fragments arranged by size:
2720 459 209 133
TatI w'GTAC_w
Cuts at: 3036 3036
Size: 3521
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 3038 3070 3038
Size: 32 3489
Tth111I GACn'n_nGTC
Cuts at: 1318 1318
Size: 3521
XbaI T'CTAG_A
Cuts at: 552 552
Size: 3521
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 339 1016 339
Size: 677 2844
XmaI C'CCGG_G
Cuts at: 287 541 287
Size: 254 3267
Enzymes that do cut:
AccI Acc65I AclI AcuI AflIII AgeI AlwNI ApaLI
ApoI AseI AsiSI AvaI BamHI BanI BanII BbeI
BcgI BciVI BclI BglII BlpI Bme1580I BmrI BplI
BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BseYI
BsiEI BsiHKAI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI
BsrDI BsrFI BssSI BstAPI BstBI BstYI BtgI BtgZI
BtsI DraI DrdI EaeI EagI EarI EciI EcoICRI
Eco57MI EcoNI EcoO109I EcoRI FalI FspI HaeII Hin4I
HincII KasI KpnI MluI MmeI MscI MslI MspA1I
NaeI NarI NcoI NgoMIV NspI PciI PmeI PpiI
PstI PvuI PvuII RsrII SacI SalI SapI SbfI
ScaI SfcI SfoI SgfI SmaI SmlI SpeI SphI
StuI StyI TaqII TatI TstI Tth111I XbaI XcmI
XmaI
Enzymes that do not cut:
AarI AatII AfeI AflII AhdI AleI AlfI AloI
ApaI AscI AvrII BaeI BbsI BbvCI BfrBI BglI
BmgBI BmtI Bpu10I BsaXI BseRI BsgI BsiWI BsmI
BsrGI BssHII BstEII BstXI BstZ17I Bsu36I ClaI CspCI
DraIII EcoRV FseI FspAI HindIII HpaI MfeI NdeI
NheI NotI NruI NsiI PacI PasI PflMI PfoI
PmlI PpuMI PshAI PsiI PspOMI PspXI PsrI SacII
SanDI SexAI SfiI SgrAI SnaBI SrfI SspI SwaI
XhoI XmnI ZraI