Restriction map for
pF1KE0111
(Circular) (Six-base) MAPSORT of: pF1KE0111.seq Check: 7444 from: 1 to: 4151
pF1KE0111 4151 bp
With 182 enzymes: SgfI *
April 24, 2009 13:10 ..
AccI GT'mk_AC
Cuts at: 1189 1189
Size: 4151
Acc65I G'GTAC_C
Cuts at: 1167 1167
Size: 4151
AclI AA'CG_TT
Cuts at: 3991 3991
Size: 4151
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 241 435 1976 2408 3155 3423 241
Size: 194 1541 432 747 268 969
Fragments arranged by size:
1541 969 747 432 268 194
AfeI AGC'GCT
Cuts at: 448 448
Size: 4151
AflIII A'CryG_T
Cuts at: 1503 2607 3584 1503
Size: 1104 977 2070
Fragments arranged by size:
2070 1104 977
AgeI A'CCGG_T
Cuts at: 2539 2539
Size: 4151
AhdI GACnn_n'nnGTC
Cuts at: 161 161
Size: 4151
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 836 836
Size: 4151
AlwNI CAG_nnn'CTG
Cuts at: 589 774 960 1002 1456 3023 589
Size: 185 186 42 454 1567 1717
Fragments arranged by size:
1717 1567 454 186 185 42
ApaI G_GGCC'C
Cuts at: 121 140 252 121
Size: 19 112 4020
Fragments arranged by size:
4020 112 19
ApaLI G'TGCA_C
Cuts at: 2921 2921
Size: 4151
ApoI r'AATT_y
Cuts at: 1155 3504 3760 1155
Size: 2349 256 1546
Fragments arranged by size:
2349 1546 256
AseI AT'TA_AT
Cuts at: 20 20
Size: 4151
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 4151
AvaI C'yCGr_G
Cuts at: 132 205 349 539 1171 132
Size: 73 144 190 632 3112
Fragments arranged by size:
3112 632 190 144 73
AvrII C'CTAG_G
Cuts at: 363 363
Size: 4151
BamHI G'GATC_C
Cuts at: 1176 1176
Size: 4151
BanI G'GyrC_C
Cuts at: 200 292 1167 1581 1829 1864 200
Size: 92 875 414 248 35 2487
Fragments arranged by size:
2487 875 414 248 92 35
BanII G_rGCy'C
Cuts at: 121 140 252 744 1050 1165 2195 121
Size: 19 112 492 306 115 1030 2077
Fragments arranged by size:
2077 1030 492 306 115 112 19
BbeI G_GCGC'C
Cuts at: 204 1585 1833 204
Size: 1381 248 2522
Fragments arranged by size:
2522 1381 248
BbsI GAAGACnn'nnnn_
Cuts at: 1119 1119
Size: 4151
BciVI GTATCCnnnnn_n'
Cuts at: 2049 2810 4151 2049
Size: 761 1341 2049
Fragments arranged by size:
2049 1341 761
BclI T'GATC_A
Cuts at: 1671 1671
Size: 4151
BglI GCCn_nnn'nGGC
Cuts at: 323 323
Size: 4151
BglII A'GATC_T
Cuts at: 1106 1666 3703 1106
Size: 560 2037 1554
Fragments arranged by size:
2037 1554 560
BlpI GC'TnA_GC
Cuts at: 1064 1263 1064
Size: 199 3952
Bme1580I G_kGCm'C
Cuts at: 121 140 252 1776 1869 2925 121
Size: 19 112 1524 93 1056 1347
Fragments arranged by size:
1524 1347 1056 112 93 19
BmgBI CAC'GTC
Cuts at: 336 336
Size: 4151
BmrI ACTGGGnnnn_n'
Cuts at: 1363 1444 1779 3923 1363
Size: 81 335 2144 1591
Fragments arranged by size:
2144 1591 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 375 2518 375
Size: 2143 2008
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 328 541 967 328
Size: 213 426 3512
Fragments arranged by size:
3512 426 213
Bpu10I CC'TnA_GC
Cuts at: 683 683
Size: 4151
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 179 367 1327 2206 2698 2996 179
Size: 188 960 879 492 298 1334
Fragments arranged by size:
1334 960 879 492 298 188
BsaI GGTCTCn'nnnn_
Cuts at: 3855 3855
Size: 4151
BsaAI yAC'GTr
Cuts at: 1504 1504
Size: 4151
BsaBI GATnn'nnATC
Cuts at: 6 1665 3702 6
Size: 1659 2037 455
Fragments arranged by size:
2037 1659 455
BsaHI Gr'CG_yC
Cuts at: 201 326 1582 1830 2532 4028 201
Size: 125 1256 248 702 1496 324
Fragments arranged by size:
1496 1256 702 324 248 125
BsaWI w'CCGG_w
Cuts at: 1073 1340 1861 2539 2813 2960 3371 1073
Size: 267 521 678 274 147 411 1853
Fragments arranged by size:
1853 678 521 411 274 267 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 249 279 249
Size: 30 4121
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1108 1108
Size: 4151
BseYI C'CCAG_C
Cuts at: 551 656 1027 1576 2334 2911 3407 551
Size: 105 371 549 758 577 496 1295
Fragments arranged by size:
1295 758 577 549 496 371 105
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1105 1105
Size: 4151
BsiEI CG_ry'CG
Cuts at: 104 184 246 1693 1739 2947 104
Size: 80 62 1447 46 1208 1308
Fragments arranged by size:
1447 1308 1208 80 62 46
BsiHKAI G_wGCw'C
Cuts at: 815 1165 1943 2133 2925 815
Size: 350 778 190 792 2041
Fragments arranged by size:
2041 792 778 350 190
BsmI GAATG_Cn'
Cuts at: 753 753
Size: 4151
Bsp1286I G_dGCh'C
Cuts at: 121 140 252 744 815 1050 1165 1776 1869
Size: 19 112 492 71 235 115 611 93
Cuts at: 1869 1943 2133 2195 2925 121
Size: 74 190 62 730 1347
Fragments arranged by size:
1347 730 611 492 235 190 115 112
93 74 71 62 19
BspEI T'CCGG_A
Cuts at: 3371 3371
Size: 4151
BspHI T'CATG_A
Cuts at: 3327 3327
Size: 4151
BspMI ACCTGCnnnn'nnnn_
Cuts at: 759 1077 1201 1415 1717 2098 759
Size: 318 124 214 302 381 2812
Fragments arranged by size:
2812 381 318 302 214 124
BsrBI CCG'CTC
Cuts at: 50 265 2443 2497 3981 4146 50
Size: 215 2178 54 1484 165 55
Fragments arranged by size:
2178 1484 215 165 55 54
BsrDI GCAATG_nn'
Cuts at: 2063 2063
Size: 4151
BsrFI r'CCGG_y
Cuts at: 2149 2330 2539 2149
Size: 181 209 3761
Fragments arranged by size:
3761 209 181
BssSI C'ACGA_G
Cuts at: 725 2422 2780 725
Size: 1697 358 2096
Fragments arranged by size:
2096 1697 358
BstAPI GCAn_nnn'nTGC
Cuts at: 730 1528 730
Size: 798 3353
BstBI TT'CG_AA
Cuts at: 2512 2512
Size: 4151
BstXI CCAn_nnnn'nTGG
Cuts at: 565 565
Size: 4151
BstYI r'GATC_y
Cuts at: 1 302 356 1106 1176 1643 1666 2001 2247
Size: 301 54 750 70 467 23 335 246
Cuts at: 2247 3259 3345 3357 3703 1
Size: 1012 86 12 346 449
Fragments arranged by size:
1012 750 467 449 346 335 301 246
86 70 54 23 12
Bsu36I CC'TnA_GG
Cuts at: 398 421 398
Size: 23 4128
BtgI C'CryG_G
Cuts at: 270 270
Size: 4151
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 2101 2282 2445 2101
Size: 181 163 3807
Fragments arranged by size:
3807 181 163
BtsI GCAGTG_nn'
Cuts at: 763 1394 763
Size: 631 3520
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 833 868 833
Size: 35 4116
DraI TTT'AAA
Cuts at: 1150 1150
Size: 4151
DrdI GACnn_nn'nnGTC
Cuts at: 1857 2715 1857
Size: 858 3293
EaeI y'GGCC_r
Cuts at: 181 315 1736 1910 2301 2328 3673 181
Size: 134 1421 174 391 27 1345 659
Fragments arranged by size:
1421 1345 659 391 174 134 27
EagI C'GGCC_G
Cuts at: 181 1736 181
Size: 1555 2596
EarI CTCTTCn'nnn_
Cuts at: 2174 2384 2174
Size: 210 3941
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 441 2359 2669 2815 3961 441
Size: 1918 310 146 1146 631
Fragments arranged by size:
1918 1146 631 310 146
EcoICRI GAG'CTC
Cuts at: 1163 1163
Size: 4151
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 241 328 435 541 967 1976 2408 3155 3423
Size: 87 107 106 426 1009 432 747 268
Cuts at: 3423 241
Size: 969
Fragments arranged by size:
1009 969 747 432 426 268 107 106
87
EcoO109I rG'GnC_Cy
Cuts at: 117 136 249 367 374 393 491 673 771
Size: 19 113 118 7 19 98 182 98
Cuts at: 771 867 1290 117
Size: 96 423 2978
Fragments arranged by size:
2978 423 182 118 113 98 98 96
19 19 7
EcoRI G'AATT_C
Cuts at: 1155 1155
Size: 4151
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 246 2170 246
Size: 1924 2227
FspI TGC'GCA
Cuts at: 1932 1932
Size: 4151
HaeII r_GCGC'y
Cuts at: 204 450 513 1585 1833 2855 3834 204
Size: 246 63 1072 248 1022 979 521
Fragments arranged by size:
1072 1022 979 521 248 246 63
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 106 138 147 177 179 209 1098 1130 1993
Size: 32 9 30 2 30 889 32 863
Cuts at: 1993 2023 2025 2055 106
Size: 30 2 30 2202
Fragments arranged by size:
2202 889 863 32 32 30 30 30
30 9 2 2
HincII GTy'rAC
Cuts at: 1190 1190
Size: 4151
KasI G'GCGC_C
Cuts at: 200 1581 1829 200
Size: 1381 248 2522
Fragments arranged by size:
2522 1381 248
KpnI G_GTAC'C
Cuts at: 1171 1171
Size: 4151
MluI A'CGCG_T
Cuts at: 3584 3584
Size: 4151
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 266 836 2822 3006 266
Size: 570 1986 184 1411
Fragments arranged by size:
1986 1411 570 184
MscI TGG'CCA
Cuts at: 1912 1912
Size: 4151
MslI CAynn'nnrTG
Cuts at: 818 940 2140 2267 818
Size: 122 1200 127 2702
Fragments arranged by size:
2702 1200 127 122
MspA1I CmG'CkG
Cuts at: 617 798 957 999 1262 1576 1936 2949 3194
Size: 181 159 42 263 314 360 1013 245
Cuts at: 3194 617
Size: 1574
Fragments arranged by size:
1574 1013 360 314 263 245 181 159
42
NaeI GCC'GGC
Cuts at: 2332 2332
Size: 4151
NarI GG'CG_CC
Cuts at: 201 1582 1830 201
Size: 1381 248 2522
Fragments arranged by size:
2522 1381 248
NgoMIV G'CCGG_C
Cuts at: 2330 2330
Size: 4151
NspI r_CATG'y
Cuts at: 945 1204 1704 2611 945
Size: 259 500 907 2485
Fragments arranged by size:
2485 907 500 259
PciI A'CATG_T
Cuts at: 2607 2607
Size: 4151
PflMI CCAn_nnn'nTGG
Cuts at: 960 960
Size: 4151
PfoI T'CCnGG_A
Cuts at: 305 557 305
Size: 252 3899
PmeI GTTT'AAAC
Cuts at: 1150 1150
Size: 4151
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3290 3322 3290
Size: 32 4119
PpuMI rG'GwC_Cy
Cuts at: 367 393 673 771 867 367
Size: 26 280 98 96 3651
Fragments arranged by size:
3651 280 98 96 26
PspOMI G'GGCC_C
Cuts at: 117 136 248 117
Size: 19 112 4020
Fragments arranged by size:
4020 112 19
PspXI vC'TCGA_Gb
Cuts at: 205 349 205
Size: 144 4007
PstI C_TGCA'G
Cuts at: 770 1198 1883 770
Size: 428 685 3038
Fragments arranged by size:
3038 685 428
PvuI CG_AT'CG
Cuts at: 104 104
Size: 4151
PvuII CAG'CTG
Cuts at: 999 1576 1936 999
Size: 577 360 3214
Fragments arranged by size:
3214 577 360
RsrII CG'GwC_CG
Cuts at: 2346 2346
Size: 4151
SacI G_AGCT'C
Cuts at: 1165 1165
Size: 4151
SalI G'TCGA_C
Cuts at: 1188 1188
Size: 4151
SapI GCTCTTCn'nnn_
Cuts at: 2174 2384 2174
Size: 210 3941
SbfI CC_TGCA'GG
Cuts at: 1198 1198
Size: 4151
ScaI AGT'ACT
Cuts at: 3668 3668
Size: 4151
SexAI A'CCwGG_T
Cuts at: 669 669
Size: 4151
SfcI C'TryA_G
Cuts at: 33 174 766 1194 1879 2872 3063 33
Size: 141 592 428 685 993 191 1121
Fragments arranged by size:
1121 993 685 592 428 191 141
SfoI GGC'GCC
Cuts at: 202 1583 1831 202
Size: 1381 248 2522
Fragments arranged by size:
2522 1381 248
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 4151
SmaI CCC'GGG
Cuts at: 541 1173 541
Size: 632 3519
SmlI C'TyrA_G
Cuts at: 194 205 349 382 1306 2221 2713 2975 194
Size: 11 144 33 924 915 492 262 1370
Fragments arranged by size:
1370 924 915 492 262 144 33 11
SpeI A'CTAG_T
Cuts at: 67 67
Size: 4151
SphI G_CATG'C
Cuts at: 945 1204 1704 945
Size: 259 500 3392
Fragments arranged by size:
3392 500 259
StuI AGG'CCT
Cuts at: 695 695
Size: 4151
StyI C'CwwG_G
Cuts at: 363 1285 1639 363
Size: 922 354 2875
Fragments arranged by size:
2875 922 354
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 274 531 1734 1867 2326 2535 274
Size: 257 1203 133 459 209 1890
Fragments arranged by size:
1890 1203 459 257 209 133
TatI w'GTAC_w
Cuts at: 3666 3666
Size: 4151
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 249 281 755 787 3668 3700 249
Size: 32 474 32 2881 32 700
Fragments arranged by size:
2881 700 474 32 32 32
Tth111I GACn'n_nGTC
Cuts at: 585 1948 585
Size: 1363 2788
XbaI T'CTAG_A
Cuts at: 1182 1182
Size: 4151
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 1646 1646
Size: 4151
XhoI C'TCGA_G
Cuts at: 205 349 205
Size: 144 4007
XmaI C'CCGG_G
Cuts at: 539 1171 539
Size: 632 3519
XmnI GAAnn'nnTTC
Cuts at: 448 448
Size: 4151
Enzymes that do cut:
AccI Acc65I AclI AcuI AfeI AflIII AgeI AhdI
AlfI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI
AvrII BamHI BanI BanII BbeI BbsI BciVI BclI
BglI BglII BlpI Bme1580I BmgBI BmrI BplI BpmI
Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI
BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI
BspHI BspMI BsrBI BsrDI BsrFI BssSI BstAPI BstBI
BstXI BstYI Bsu36I BtgI BtgZI BtsI CspCI DraI
DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoO109I
EcoRI FalI FspI HaeII Hin4I HincII KasI KpnI
MluI MmeI MscI MslI MspA1I NaeI NarI NgoMIV
NspI PciI PflMI PfoI PmeI PpiI PpuMI PspOMI
PspXI PstI PvuI PvuII RsrII SacI SalI SapI
SbfI ScaI SexAI SfcI SfoI SgfI SmaI SmlI
SpeI SphI StuI StyI TaqII TatI TstI Tth111I
XbaI XcmI XhoI XmaI XmnI
Enzymes that do not cut:
AarI AatII AflII AleI AloI AscI BaeI BbvCI
BcgI BfrBI BmtI BsiWI BsmBI BsrGI BssHII BstEII
BstZ17I ClaI DraIII EcoNI EcoRV FseI FspAI HindIII
HpaI MfeI NcoI NdeI NheI NotI NruI NsiI
PacI PasI PmlI PshAI PsiI PsrI SacII SanDI
SfiI SgrAI SnaBI SrfI SspI SwaI ZraI