Restriction map for
pF1KE0115
(Circular) (Six-base) MAPSORT of: pF1KE0115.seq Check: 4396 from: 1 to: 3797
pF1KE0115 3797 bp
With 182 enzymes: SgfI *
April 24, 2009 13:10 ..
AccI GT'mk_AC
Cuts at: 835 835
Size: 3797
Acc65I G'GTAC_C
Cuts at: 813 813
Size: 3797
AclI AA'CG_TT
Cuts at: 3637 3637
Size: 3797
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1622 2054 2801 3069 1622
Size: 432 747 268 2350
Fragments arranged by size:
2350 747 432 268
AflII C'TTAA_G
Cuts at: 549 690 549
Size: 141 3656
AflIII A'CryG_T
Cuts at: 616 674 1149 2253 3230 616
Size: 58 475 1104 977 1183
Fragments arranged by size:
1183 1104 977 475 58
AgeI A'CCGG_T
Cuts at: 2185 2185
Size: 3797
AlwNI CAG_nnn'CTG
Cuts at: 1102 2669 1102
Size: 1567 2230
ApaLI G'TGCA_C
Cuts at: 2567 2567
Size: 3797
ApoI r'AATT_y
Cuts at: 234 630 801 3150 3406 234
Size: 396 171 2349 256 625
Fragments arranged by size:
2349 625 396 256 171
AseI AT'TA_AT
Cuts at: 20 20
Size: 3797
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 3797
AvaI C'yCGr_G
Cuts at: 817 817
Size: 3797
BamHI G'GATC_C
Cuts at: 189 822 189
Size: 633 3164
BanI G'GyrC_C
Cuts at: 813 1227 1475 1510 813
Size: 414 248 35 3100
Fragments arranged by size:
3100 414 248 35
BanII G_rGCy'C
Cuts at: 811 1841 811
Size: 1030 2767
BbeI G_GCGC'C
Cuts at: 1231 1479 1231
Size: 248 3549
BbsI GAAGACnn'nnnn_
Cuts at: 691 691
Size: 3797
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 90 124 90
Size: 34 3763
BciVI GTATCCnnnnn_n'
Cuts at: 1695 2456 3797 1695
Size: 761 1341 1695
Fragments arranged by size:
1695 1341 761
BclI T'GATC_A
Cuts at: 1317 1317
Size: 3797
BglII A'GATC_T
Cuts at: 1312 3349 1312
Size: 2037 1760
BlpI GC'TnA_GC
Cuts at: 909 909
Size: 3797
Bme1580I G_kGCm'C
Cuts at: 1422 1515 2571 1422
Size: 93 1056 2648
Fragments arranged by size:
2648 1056 93
BmrI ACTGGGnnnn_n'
Cuts at: 1009 1090 1425 3569 1009
Size: 81 335 2144 1237
Fragments arranged by size:
2144 1237 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2164 2164
Size: 3797
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 404 404
Size: 3797
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 364 973 1852 2344 2642 364
Size: 609 879 492 298 1519
Fragments arranged by size:
1519 879 609 492 298
BsaI GGTCTCn'nnnn_
Cuts at: 3501 3501
Size: 3797
BsaAI yAC'GTr
Cuts at: 617 1150 617
Size: 533 3264
BsaBI GATnn'nnATC
Cuts at: 6 1311 3348 6
Size: 1305 2037 455
Fragments arranged by size:
2037 1305 455
BsaHI Gr'CG_yC
Cuts at: 1228 1476 2178 3674 1228
Size: 248 702 1496 1351
Fragments arranged by size:
1496 1351 702 248
BsaWI w'CCGG_w
Cuts at: 986 1507 2185 2459 2606 3017 986
Size: 521 678 274 147 411 1766
Fragments arranged by size:
1766 678 521 411 274 147
BseYI C'CCAG_C
Cuts at: 1222 1980 2557 3053 1222
Size: 758 577 496 1966
Fragments arranged by size:
1966 758 577 496
BsiEI CG_ry'CG
Cuts at: 104 1339 1385 2593 104
Size: 1235 46 1208 1308
Fragments arranged by size:
1308 1235 1208 46
BsiHKAI G_wGCw'C
Cuts at: 811 1589 1779 2571 811
Size: 778 190 792 2037
Fragments arranged by size:
2037 792 778 190
Bsp1286I G_dGCh'C
Cuts at: 811 1422 1515 1589 1779 1841 2571 811
Size: 611 93 74 190 62 730 2037
Fragments arranged by size:
2037 730 611 190 93 74 62
BspEI T'CCGG_A
Cuts at: 3017 3017
Size: 3797
BspHI T'CATG_A
Cuts at: 2973 2973
Size: 3797
BspMI ACCTGCnnnn'nnnn_
Cuts at: 847 1061 1363 1744 847
Size: 214 302 381 2900
Fragments arranged by size:
2900 381 302 214
BsrBI CCG'CTC
Cuts at: 50 2089 2143 3627 3792 50
Size: 2039 54 1484 165 55
Fragments arranged by size:
2039 1484 165 55 54
BsrDI GCAATG_nn'
Cuts at: 1709 1709
Size: 3797
BsrFI r'CCGG_y
Cuts at: 1795 1976 2185 1795
Size: 181 209 3407
Fragments arranged by size:
3407 209 181
BssSI C'ACGA_G
Cuts at: 2068 2426 2068
Size: 358 3439
BstAPI GCAn_nnn'nTGC
Cuts at: 1174 1174
Size: 3797
BstBI TT'CG_AA
Cuts at: 2158 2158
Size: 3797
BstXI CCAn_nnnn'nTGG
Cuts at: 621 621
Size: 3797
BstYI r'GATC_y
Cuts at: 1 189 719 822 1289 1312 1647 1893 2905
Size: 188 530 103 467 23 335 246 1012
Cuts at: 2905 2991 3003 3349 1
Size: 86 12 346 449
Fragments arranged by size:
1012 530 467 449 346 335 246 188
103 86 23 12
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 1747 1928 2091 1747
Size: 181 163 3453
Fragments arranged by size:
3453 181 163
BtsI GCAGTG_nn'
Cuts at: 748 1040 748
Size: 292 3505
DraI TTT'AAA
Cuts at: 440 796 440
Size: 356 3441
DrdI GACnn_nn'nnGTC
Cuts at: 1503 2361 1503
Size: 858 2939
EaeI y'GGCC_r
Cuts at: 1382 1556 1947 1974 3319 1382
Size: 174 391 27 1345 1860
Fragments arranged by size:
1860 1345 391 174 27
EagI C'GGCC_G
Cuts at: 1382 1382
Size: 3797
EarI CTCTTCn'nnn_
Cuts at: 1820 2030 1820
Size: 210 3587
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 2005 2315 2461 3607 2005
Size: 310 146 1146 2195
Fragments arranged by size:
2195 1146 310 146
EcoICRI GAG'CTC
Cuts at: 809 809
Size: 3797
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 404 1622 2054 2801 3069 404
Size: 1218 432 747 268 1132
Fragments arranged by size:
1218 1132 747 432 268
EcoNI CCTnn'n_nnAGG
Cuts at: 577 577
Size: 3797
EcoO109I rG'GnC_Cy
Cuts at: 936 936
Size: 3797
EcoRI G'AATT_C
Cuts at: 801 801
Size: 3797
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 487 801 1816 487
Size: 314 1015 2468
Fragments arranged by size:
2468 1015 314
FspI TGC'GCA
Cuts at: 1578 1578
Size: 3797
HaeII r_GCGC'y
Cuts at: 1231 1479 2501 3480 1231
Size: 248 1022 979 1548
Fragments arranged by size:
1548 1022 979 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1639 1669 1671 1701 1639
Size: 30 2 30 3735
Fragments arranged by size:
3735 30 30 2
HincII GTy'rAC
Cuts at: 836 836
Size: 3797
HindIII A'AGCT_T
Cuts at: 546 546
Size: 3797
KasI G'GCGC_C
Cuts at: 1227 1475 1227
Size: 248 3549
KpnI G_GTAC'C
Cuts at: 817 817
Size: 3797
MfeI C'AATT_G
Cuts at: 117 117
Size: 3797
MluI A'CGCG_T
Cuts at: 3230 3230
Size: 3797
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 361 2468 2652 361
Size: 2107 184 1506
Fragments arranged by size:
2107 1506 184
MscI TGG'CCA
Cuts at: 1558 1558
Size: 3797
MslI CAynn'nnrTG
Cuts at: 482 1786 1913 482
Size: 1304 127 2366
Fragments arranged by size:
2366 1304 127
MspA1I CmG'CkG
Cuts at: 424 908 1222 1582 2595 2840 424
Size: 484 314 360 1013 245 1381
Fragments arranged by size:
1381 1013 484 360 314 245
NaeI GCC'GGC
Cuts at: 1978 1978
Size: 3797
NarI GG'CG_CC
Cuts at: 1228 1476 1228
Size: 248 3549
NgoMIV G'CCGG_C
Cuts at: 1976 1976
Size: 3797
NspI r_CATG'y
Cuts at: 678 850 1350 2257 678
Size: 172 500 907 2218
Fragments arranged by size:
2218 907 500 172
PciI A'CATG_T
Cuts at: 674 2253 674
Size: 1579 2218
PmeI GTTT'AAAC
Cuts at: 796 796
Size: 3797
PmlI CAC'GTG
Cuts at: 617 617
Size: 3797
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2936 2968 2936
Size: 32 3765
PsrI GAACnnnnnnTACnnnnnnn_nnnnn'
Cuts at: 662 694 662
Size: 32 3765
PstI C_TGCA'G
Cuts at: 844 1529 844
Size: 685 3112
PvuI CG_AT'CG
Cuts at: 104 104
Size: 3797
PvuII CAG'CTG
Cuts at: 424 1222 1582 424
Size: 798 360 2639
Fragments arranged by size:
2639 798 360
RsrII CG'GwC_CG
Cuts at: 1992 1992
Size: 3797
SacI G_AGCT'C
Cuts at: 811 811
Size: 3797
SalI G'TCGA_C
Cuts at: 834 834
Size: 3797
SapI GCTCTTCn'nnn_
Cuts at: 1820 2030 1820
Size: 210 3587
SbfI CC_TGCA'GG
Cuts at: 844 844
Size: 3797
ScaI AGT'ACT
Cuts at: 467 3314 467
Size: 2847 950
SfcI C'TryA_G
Cuts at: 33 840 1525 2518 2709 33
Size: 807 685 993 191 1121
Fragments arranged by size:
1121 993 807 685 191
SfoI GGC'GCC
Cuts at: 1229 1477 1229
Size: 248 3549
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 3797
SmaI CCC'GGG
Cuts at: 819 819
Size: 3797
SmlI C'TyrA_G
Cuts at: 379 549 690 952 1867 2359 2621 379
Size: 170 141 262 915 492 262 1555
Fragments arranged by size:
1555 915 492 262 262 170 141
SpeI A'CTAG_T
Cuts at: 67 67
Size: 3797
SphI G_CATG'C
Cuts at: 850 1350 850
Size: 500 3297
StyI C'CwwG_G
Cuts at: 704 931 1285 704
Size: 227 354 3216
Fragments arranged by size:
3216 354 227
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 1380 1513 1972 2181 1380
Size: 133 459 209 2996
Fragments arranged by size:
2996 459 209 133
TatI w'GTAC_w
Cuts at: 465 3312 465
Size: 2847 950
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 3314 3346 3314
Size: 32 3765
Tth111I GACn'n_nGTC
Cuts at: 1594 1594
Size: 3797
XbaI T'CTAG_A
Cuts at: 828 828
Size: 3797
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 1292 1292
Size: 3797
XmaI C'CCGG_G
Cuts at: 817 817
Size: 3797
XmnI GAAnn'nnTTC
Cuts at: 212 212
Size: 3797
Enzymes that do cut:
AccI Acc65I AclI AcuI AflII AflIII AgeI AlwNI
ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII
BbeI BbsI BcgI BciVI BclI BglII BlpI Bme1580I
BmrI BplI BpmI BpuEI BsaI BsaAI BsaBI BsaHI
BsaWI BseYI BsiEI BsiHKAI Bsp1286I BspEI BspHI BspMI
BsrBI BsrDI BsrFI BssSI BstAPI BstBI BstXI BstYI
BtgZI BtsI DraI DrdI EaeI EagI EarI EciI
EcoICRI Eco57MI EcoNI EcoO109I EcoRI FalI FspI HaeII
Hin4I HincII HindIII KasI KpnI MfeI MluI MmeI
MscI MslI MspA1I NaeI NarI NgoMIV NspI PciI
PmeI PmlI PpiI PsrI PstI PvuI PvuII RsrII
SacI SalI SapI SbfI ScaI SfcI SfoI SgfI
SmaI SmlI SpeI SphI StyI TaqII TatI TstI
Tth111I XbaI XcmI XmaI XmnI
Enzymes that do not cut:
AarI AatII AfeI AhdI AleI AlfI AloI ApaI
AscI AvrII BaeI BbvCI BfrBI BglI BmgBI BmtI
Bpu10I BsaXI BseRI BsgI BsiWI BsmI BsmBI BsrGI
BssHII BstEII BstZ17I Bsu36I BtgI ClaI CspCI DraIII
EcoRV FseI FspAI HpaI NcoI NdeI NheI NotI
NruI NsiI PacI PasI PflMI PfoI PpuMI PshAI
PsiI PspOMI PspXI SacII SanDI SexAI SfiI SgrAI
SnaBI SrfI SspI StuI SwaI XhoI ZraI