Restriction map for
pF1KE0116
(Circular) (Six-base) MAPSORT of: pF1KE0116.seq Check: 5883 from: 1 to: 4283
pF1KE0116 4283 bp
With 182 enzymes: SgfI *
April 24, 2009 13:10 ..
AccI GT'mk_AC
Cuts at: 1321 1321
Size: 4283
Acc65I G'GTAC_C
Cuts at: 716 1299 716
Size: 583 3700
AclI AA'CG_TT
Cuts at: 4123 4123
Size: 4283
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 284 424 640 736 1161 2108 2540 3287 3555
Size: 140 216 96 425 947 432 747 268
Cuts at: 3555 284
Size: 1012
Fragments arranged by size:
1012 947 747 432 425 268 216 140
96
AflIII A'CryG_T
Cuts at: 1635 2739 3716 1635
Size: 1104 977 2202
Fragments arranged by size:
2202 1104 977
AgeI A'CCGG_T
Cuts at: 2671 2671
Size: 4283
AlwNI CAG_nnn'CTG
Cuts at: 440 1588 3155 440
Size: 1148 1567 1568
Fragments arranged by size:
1568 1567 1148
ApaLI G'TGCA_C
Cuts at: 145 3053 145
Size: 2908 1375
ApoI r'AATT_y
Cuts at: 1038 1116 1287 3636 3892 1038
Size: 78 171 2349 256 1429
Fragments arranged by size:
2349 1429 256 171 78
AseI AT'TA_AT
Cuts at: 20 20
Size: 4283
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 4283
AvaI C'yCGr_G
Cuts at: 1190 1209 1303 1190
Size: 19 94 4170
Fragments arranged by size:
4170 94 19
AvrII C'CTAG_G
Cuts at: 489 489
Size: 4283
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'
Cuts at: 114 147 114
Size: 33 4250
BamHI G'GATC_C
Cuts at: 1308 1308
Size: 4283
BanI G'GyrC_C
Cuts at: 716 1299 1713 1961 1996 716
Size: 583 414 248 35 3003
Fragments arranged by size:
3003 583 414 248 35
BanII G_rGCy'C
Cuts at: 1085 1297 2327 1085
Size: 212 1030 3041
Fragments arranged by size:
3041 1030 212
BbeI G_GCGC'C
Cuts at: 1717 1965 1717
Size: 248 4035
BbsI GAAGACnn'nnnn_
Cuts at: 259 634 259
Size: 375 3908
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1233 1267 1233
Size: 34 4249
BciVI GTATCCnnnnn_n'
Cuts at: 2181 2942 4283 2181
Size: 761 1341 2181
Fragments arranged by size:
2181 1341 761
BclI T'GATC_A
Cuts at: 1803 1803
Size: 4283
BfrBI ATG'CAT
Cuts at: 1128 1128
Size: 4283
BglI GCCn_nnn'nGGC
Cuts at: 241 847 241
Size: 606 3677
BglII A'GATC_T
Cuts at: 1798 3835 1798
Size: 2037 2246
BlpI GC'TnA_GC
Cuts at: 1395 1395
Size: 4283
Bme1580I G_kGCm'C
Cuts at: 149 1908 2001 3057 149
Size: 1759 93 1056 1375
Fragments arranged by size:
1759 1375 1056 93
BmgBI CAC'GTC
Cuts at: 144 144
Size: 4283
BmrI ACTGGGnnnn_n'
Cuts at: 1495 1576 1911 4055 1495
Size: 81 335 2144 1723
Fragments arranged by size:
2144 1723 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 517 2650 517
Size: 2133 2150
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 181 386 1018 1126 181
Size: 205 632 108 3338
Fragments arranged by size:
3338 632 205 108
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 642 976 1069 1459 2338 2830 3128 642
Size: 334 93 390 879 492 298 1797
Fragments arranged by size:
1797 879 492 390 334 298 93
BsaI GGTCTCn'nnnn_
Cuts at: 3987 3987
Size: 4283
BsaAI yAC'GTr
Cuts at: 1636 1636
Size: 4283
BsaBI GATnn'nnATC
Cuts at: 6 1797 3834 6
Size: 1791 2037 455
Fragments arranged by size:
2037 1791 455
BsaHI Gr'CG_yC
Cuts at: 1067 1714 1962 2664 4160 1067
Size: 647 248 702 1496 1190
Fragments arranged by size:
1496 1190 702 647 248
BsaWI w'CCGG_w
Cuts at: 1133 1472 1993 2671 2945 3092 3503 1133
Size: 339 521 678 274 147 411 1913
Fragments arranged by size:
1913 678 521 411 339 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 437 467 437
Size: 30 4253
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 346 775 346
Size: 429 3854
BseYI C'CCAG_C
Cuts at: 1708 2466 3043 3539 1708
Size: 758 577 496 2452
Fragments arranged by size:
2452 758 577 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 655 655
Size: 4283
BsiEI CG_ry'CG
Cuts at: 104 1825 1871 3079 104
Size: 1721 46 1208 1308
Fragments arranged by size:
1721 1308 1208 46
BsiHKAI G_wGCw'C
Cuts at: 149 1085 1297 2075 2265 3057 149
Size: 936 212 778 190 792 1375
Fragments arranged by size:
1375 936 792 778 212 190
BsmBI CGTCTCn'nnnn_
Cuts at: 820 820
Size: 4283
Bsp1286I G_dGCh'C
Cuts at: 149 1085 1297 1908 2001 2075 2265 2327 3057
Size: 936 212 611 93 74 190 62 730
Cuts at: 3057 149
Size: 1375
Fragments arranged by size:
1375 936 730 611 212 190 93 74
62
BspEI T'CCGG_A
Cuts at: 3503 3503
Size: 4283
BspHI T'CATG_A
Cuts at: 3459 3459
Size: 4283
BspMI ACCTGCnnnn'nnnn_
Cuts at: 1333 1547 1849 2230 1333
Size: 214 302 381 3386
Fragments arranged by size:
3386 381 302 214
BsrBI CCG'CTC
Cuts at: 50 2575 2629 4113 4278 50
Size: 2525 54 1484 165 55
Fragments arranged by size:
2525 1484 165 55 54
BsrDI GCAATG_nn'
Cuts at: 2195 2195
Size: 4283
BsrFI r'CCGG_y
Cuts at: 713 2281 2462 2671 713
Size: 1568 181 209 2325
Fragments arranged by size:
2325 1568 209 181
BsrGI T'GTAC_A
Cuts at: 569 569
Size: 4283
BssSI C'ACGA_G
Cuts at: 382 2554 2912 382
Size: 2172 358 1753
Fragments arranged by size:
2172 1753 358
BstAPI GCAn_nnn'nTGC
Cuts at: 642 1660 642
Size: 1018 3265
BstBI TT'CG_AA
Cuts at: 2644 2644
Size: 4283
BstXI CCAn_nnnn'nTGG
Cuts at: 283 283
Size: 4283
BstYI r'GATC_y
Cuts at: 1 968 1308 1775 1798 2133 2379 3391 3477
Size: 967 340 467 23 335 246 1012 86
Cuts at: 3477 3489 3835 1
Size: 12 346 449
Fragments arranged by size:
1012 967 467 449 346 340 335 246
86 23 12
Bsu36I CC'TnA_GG
Cuts at: 584 584
Size: 4283
BtgI C'CryG_G
Cuts at: 263 263
Size: 4283
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 2233 2414 2577 2233
Size: 181 163 3939
Fragments arranged by size:
3939 181 163
BtsI GCAGTG_nn'
Cuts at: 1526 1526
Size: 4283
DraI TTT'AAA
Cuts at: 1282 1282
Size: 4283
DrdI GACnn_nn'nnGTC
Cuts at: 1989 2847 1989
Size: 858 3425
EaeI y'GGCC_r
Cuts at: 647 950 1868 2042 2433 2460 3805 647
Size: 303 918 174 391 27 1345 1125
Fragments arranged by size:
1345 1125 918 391 303 174 27
EagI C'GGCC_G
Cuts at: 1868 1868
Size: 4283
EarI CTCTTCn'nnn_
Cuts at: 400 2306 2516 400
Size: 1906 210 2167
Fragments arranged by size:
2167 1906 210
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 2491 2801 2947 4093 2491
Size: 310 146 1146 2681
Fragments arranged by size:
2681 1146 310 146
EcoICRI GAG'CTC
Cuts at: 1083 1295 1083
Size: 212 4071
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 181 284 386 424 640 736 1018 1126 1161
Size: 103 102 38 216 96 282 108 35
Cuts at: 1161 2108 2540 3287 3555 181
Size: 947 432 747 268 909
Fragments arranged by size:
947 909 747 432 282 268 216 108
103 102 96 38 35
EcoNI CCTnn'n_nnAGG
Cuts at: 940 940
Size: 4283
EcoO109I rG'GnC_Cy
Cuts at: 1422 1422
Size: 4283
EcoRI G'AATT_C
Cuts at: 1038 1287 1038
Size: 249 4034
EcoRV GAT'ATC
Cuts at: 1226 1226
Size: 4283
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 366 2302 366
Size: 1936 2347
FspI TGC'GCA
Cuts at: 2064 2064
Size: 4283
HaeII r_GCGC'y
Cuts at: 813 1717 1965 2987 3966 813
Size: 904 248 1022 979 1130
Fragments arranged by size:
1130 1022 979 904 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 819 851 2125 2155 2157 2187 819
Size: 32 1274 30 2 30 2915
Fragments arranged by size:
2915 1274 32 30 30 2
HincII GTy'rAC
Cuts at: 1322 1322
Size: 4283
HindIII A'AGCT_T
Cuts at: 475 652 475
Size: 177 4106
KasI G'GCGC_C
Cuts at: 1713 1961 1713
Size: 248 4035
KpnI G_GTAC'C
Cuts at: 720 1303 720
Size: 583 3700
MluI A'CGCG_T
Cuts at: 3716 3716
Size: 4283
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 774 2954 3138 774
Size: 2180 184 1919
Fragments arranged by size:
2180 1919 184
MscI TGG'CCA
Cuts at: 649 2044 649
Size: 1395 2888
MslI CAynn'nnrTG
Cuts at: 343 980 1264 2272 2399 343
Size: 637 284 1008 127 2227
Fragments arranged by size:
2227 1008 637 284 127
MspA1I CmG'CkG
Cuts at: 283 402 1005 1394 1708 2068 3081 3326 283
Size: 119 603 389 314 360 1013 245 1240
Fragments arranged by size:
1240 1013 603 389 360 314 245 119
NaeI GCC'GGC
Cuts at: 2464 2464
Size: 4283
NarI GG'CG_CC
Cuts at: 1714 1962 1714
Size: 248 4035
NcoI C'CATG_G
Cuts at: 263 263
Size: 4283
NgoMIV G'CCGG_C
Cuts at: 2462 2462
Size: 4283
NsiI A_TGCA'T
Cuts at: 1130 1130
Size: 4283
NspI r_CATG'y
Cuts at: 990 1336 1836 2743 990
Size: 346 500 907 2530
Fragments arranged by size:
2530 907 500 346
PciI A'CATG_T
Cuts at: 2739 2739
Size: 4283
PflMI CCAn_nnn'nTGG
Cuts at: 978 978
Size: 4283
PfoI T'CCnGG_A
Cuts at: 425 455 1076 425
Size: 30 621 3632
Fragments arranged by size:
3632 621 30
PmeI GTTT'AAAC
Cuts at: 1282 1282
Size: 4283
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3422 3454 3422
Size: 32 4251
PstI C_TGCA'G
Cuts at: 806 1330 2015 806
Size: 524 685 3074
Fragments arranged by size:
3074 685 524
PvuI CG_AT'CG
Cuts at: 104 104
Size: 4283
PvuII CAG'CTG
Cuts at: 283 402 1708 2068 283
Size: 119 1306 360 2498
Fragments arranged by size:
2498 1306 360 119
RsrII CG'GwC_CG
Cuts at: 2478 2478
Size: 4283
SacI G_AGCT'C
Cuts at: 1085 1297 1085
Size: 212 4071
SalI G'TCGA_C
Cuts at: 1320 1320
Size: 4283
SapI GCTCTTCn'nnn_
Cuts at: 2306 2516 2306
Size: 210 4073
SbfI CC_TGCA'GG
Cuts at: 1330 1330
Size: 4283
ScaI AGT'ACT
Cuts at: 3800 3800
Size: 4283
SfcI C'TryA_G
Cuts at: 33 445 802 1326 2011 3004 3195 33
Size: 412 357 524 685 993 191 1121
Fragments arranged by size:
1121 993 685 524 412 357 191
SfoI GGC'GCC
Cuts at: 1715 1963 1715
Size: 248 4035
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 4283
SmaI CCC'GGG
Cuts at: 1192 1211 1305 1192
Size: 19 94 4170
Fragments arranged by size:
4170 94 19
SmlI C'TyrA_G
Cuts at: 621 991 1084 1438 2353 2845 3107 621
Size: 370 93 354 915 492 262 1797
Fragments arranged by size:
1797 915 492 370 354 262 93
SpeI A'CTAG_T
Cuts at: 67 67
Size: 4283
SphI G_CATG'C
Cuts at: 1336 1836 1336
Size: 500 3783
SrfI GCCC'GGGC
Cuts at: 1192 1192
Size: 4283
SspI AAT'ATT
Cuts at: 393 393
Size: 4283
StuI AGG'CCT
Cuts at: 841 841
Size: 4283
StyI C'CwwG_G
Cuts at: 263 489 1417 1771 263
Size: 226 928 354 2775
Fragments arranged by size:
2775 928 354 226
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 164 1198 1866 1999 2458 2667 164
Size: 1034 668 133 459 209 1780
Fragments arranged by size:
1780 1034 668 459 209 133
TatI w'GTAC_w
Cuts at: 347 419 569 3798 347
Size: 72 150 3229 832
Fragments arranged by size:
3229 832 150 72
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 3800 3832 3800
Size: 32 4251
Tth111I GACn'n_nGTC
Cuts at: 2080 2080
Size: 4283
XbaI T'CTAG_A
Cuts at: 1314 1314
Size: 4283
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 1778 1778
Size: 4283
XmaI C'CCGG_G
Cuts at: 1190 1209 1303 1190
Size: 19 94 4170
Fragments arranged by size:
4170 94 19
Enzymes that do cut:
AccI Acc65I AclI AcuI AflIII AgeI AlwNI ApaLI
ApoI AseI AsiSI AvaI AvrII BaeI BamHI BanI
BanII BbeI BbsI BcgI BciVI BclI BfrBI BglI
BglII BlpI Bme1580I BmgBI BmrI BplI BpmI BpuEI
BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI
BsgI BsiEI BsiHKAI BsmBI Bsp1286I BspEI BspHI BspMI
BsrBI BsrDI BsrFI BsrGI BssSI BstAPI BstBI BstXI
BstYI Bsu36I BtgI BtgZI BtsI DraI DrdI EaeI
EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI
EcoRV FalI FspI HaeII Hin4I HincII HindIII KasI
KpnI MluI MmeI MscI MslI MspA1I NaeI NarI
NcoI NgoMIV NsiI NspI PciI PflMI PfoI PmeI
PpiI PstI PvuI PvuII RsrII SacI SalI SapI
SbfI ScaI SfcI SfoI SgfI SmaI SmlI SpeI
SphI SrfI SspI StuI StyI TaqII TatI TstI
Tth111I XbaI XcmI XmaI
Enzymes that do not cut:
AarI AatII AfeI AflII AhdI AleI AlfI AloI
ApaI AscI BbvCI BmtI Bpu10I BsiWI BsmI BssHII
BstEII BstZ17I ClaI CspCI DraIII FseI FspAI HpaI
MfeI NdeI NheI NotI NruI PacI PasI PmlI
PpuMI PshAI PsiI PspOMI PspXI PsrI SacII SanDI
SexAI SfiI SgrAI SnaBI SwaI XhoI XmnI ZraI