Restriction map for
pF1KE0117
(Circular) (Six-base) MAPSORT of: pF1KE0117.seq Check: 4573 from: 1 to: 3752
pF1KE0117 3752 bp
With 182 enzymes: SgfI *
April 24, 2009 13:10 ..
AccI GT'mk_AC
Cuts at: 790 790
Size: 3752
Acc65I G'GTAC_C
Cuts at: 768 768
Size: 3752
AclI AA'CG_TT
Cuts at: 3592 3592
Size: 3752
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 692 1577 2009 2756 3024 692
Size: 885 432 747 268 1420
Fragments arranged by size:
1420 885 747 432 268
AflIII A'CryG_T
Cuts at: 435 1104 2208 3185 435
Size: 669 1104 977 1002
Fragments arranged by size:
1104 1002 977 669
AgeI A'CCGG_T
Cuts at: 2140 2140
Size: 3752
AleI CACnn'nnGTG
Cuts at: 479 479
Size: 3752
AlwNI CAG_nnn'CTG
Cuts at: 1057 2624 1057
Size: 1567 2185
ApaLI G'TGCA_C
Cuts at: 2522 2522
Size: 3752
ApoI r'AATT_y
Cuts at: 756 3105 3361 756
Size: 2349 256 1147
Fragments arranged by size:
2349 1147 256
AseI AT'TA_AT
Cuts at: 20 581 20
Size: 561 3191
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 3752
AvaI C'yCGr_G
Cuts at: 569 772 569
Size: 203 3549
BamHI G'GATC_C
Cuts at: 777 777
Size: 3752
BanI G'GyrC_C
Cuts at: 768 1182 1430 1465 768
Size: 414 248 35 3055
Fragments arranged by size:
3055 414 248 35
BanII G_rGCy'C
Cuts at: 766 1796 766
Size: 1030 2722
BbeI G_GCGC'C
Cuts at: 1186 1434 1186
Size: 248 3504
BbsI GAAGACnn'nnnn_
Cuts at: 355 355
Size: 3752
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 490 524 490
Size: 34 3718
BciVI GTATCCnnnnn_n'
Cuts at: 291 483 1650 2411 3752 291
Size: 192 1167 761 1341 291
Fragments arranged by size:
1341 1167 761 291 192
BclI T'GATC_A
Cuts at: 1272 1272
Size: 3752
BglI GCCn_nnn'nGGC
Cuts at: 112 112
Size: 3752
BglII A'GATC_T
Cuts at: 290 1267 3304 290
Size: 977 2037 738
Fragments arranged by size:
2037 977 738
BlpI GC'TnA_GC
Cuts at: 864 864
Size: 3752
Bme1580I G_kGCm'C
Cuts at: 1377 1470 2526 1377
Size: 93 1056 2603
Fragments arranged by size:
2603 1056 93
BmrI ACTGGGnnnn_n'
Cuts at: 964 1045 1380 3524 964
Size: 81 335 2144 1192
Fragments arranged by size:
2144 1192 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2119 2119
Size: 3752
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 271 271
Size: 3752
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 928 1807 2299 2597 928
Size: 879 492 298 2083
Fragments arranged by size:
2083 879 492 298
BsaI GGTCTCn'nnnn_
Cuts at: 418 3456 418
Size: 3038 714
BsaAI yAC'GTr
Cuts at: 1105 1105
Size: 3752
BsaBI GATnn'nnATC
Cuts at: 6 649 1266 3303 6
Size: 643 617 2037 455
Fragments arranged by size:
2037 643 617 455
BsaHI Gr'CG_yC
Cuts at: 1183 1431 2133 3629 1183
Size: 248 702 1496 1306
Fragments arranged by size:
1496 1306 702 248
BsaWI w'CCGG_w
Cuts at: 941 1462 2140 2414 2561 2972 941
Size: 521 678 274 147 411 1721
Fragments arranged by size:
1721 678 521 411 274 147
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 747 747
Size: 3752
BseYI C'CCAG_C
Cuts at: 662 1177 1935 2512 3008 662
Size: 515 758 577 496 1406
Fragments arranged by size:
1406 758 577 515 496
BsiEI CG_ry'CG
Cuts at: 104 1294 1340 2548 104
Size: 1190 46 1208 1308
Fragments arranged by size:
1308 1208 1190 46
BsiHKAI G_wGCw'C
Cuts at: 766 1544 1734 2526 766
Size: 778 190 792 1992
Fragments arranged by size:
1992 792 778 190
Bsp1286I G_dGCh'C
Cuts at: 766 1377 1470 1544 1734 1796 2526 766
Size: 611 93 74 190 62 730 1992
Fragments arranged by size:
1992 730 611 190 93 74 62
BspEI T'CCGG_A
Cuts at: 2972 2972
Size: 3752
BspHI T'CATG_A
Cuts at: 2928 2928
Size: 3752
BspMI ACCTGCnnnn'nnnn_
Cuts at: 380 802 1016 1318 1699 380
Size: 422 214 302 381 2433
Fragments arranged by size:
2433 422 381 302 214
BsrBI CCG'CTC
Cuts at: 50 2044 2098 3582 3747 50
Size: 1994 54 1484 165 55
Fragments arranged by size:
1994 1484 165 55 54
BsrDI GCAATG_nn'
Cuts at: 1664 1664
Size: 3752
BsrFI r'CCGG_y
Cuts at: 1750 1931 2140 1750
Size: 181 209 3362
Fragments arranged by size:
3362 209 181
BssSI C'ACGA_G
Cuts at: 514 2023 2381 514
Size: 1509 358 1885
Fragments arranged by size:
1885 1509 358
BstAPI GCAn_nnn'nTGC
Cuts at: 173 1129 173
Size: 956 2796
BstBI TT'CG_AA
Cuts at: 2113 2113
Size: 3752
BstXI CCAn_nnnn'nTGG
Cuts at: 585 585
Size: 3752
BstYI r'GATC_y
Cuts at: 1 290 322 777 1244 1267 1602 1848 2860
Size: 289 32 455 467 23 335 246 1012
Cuts at: 2860 2946 2958 3304 1
Size: 86 12 346 449
Fragments arranged by size:
1012 467 455 449 346 335 289 246
86 32 23 12
BtgI C'CryG_G
Cuts at: 107 227 302 107
Size: 120 75 3557
Fragments arranged by size:
3557 120 75
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 1702 1883 2046 1702
Size: 181 163 3408
Fragments arranged by size:
3408 181 163
BtsI GCAGTG_nn'
Cuts at: 995 995
Size: 3752
DraI TTT'AAA
Cuts at: 751 751
Size: 3752
DrdI GACnn_nn'nnGTC
Cuts at: 1458 2316 1458
Size: 858 2894
EaeI y'GGCC_r
Cuts at: 1337 1511 1902 1929 3274 1337
Size: 174 391 27 1345 1815
Fragments arranged by size:
1815 1345 391 174 27
EagI C'GGCC_G
Cuts at: 1337 1337
Size: 3752
EarI CTCTTCn'nnn_
Cuts at: 416 670 707 711 1775 1985 416
Size: 254 37 4 1064 210 2183
Fragments arranged by size:
2183 1064 254 210 37 4
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 172 1960 2270 2416 3562 172
Size: 1788 310 146 1146 362
Fragments arranged by size:
1788 1146 362 310 146
EcoICRI GAG'CTC
Cuts at: 764 764
Size: 3752
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 271 692 1577 2009 2756 3024 271
Size: 421 885 432 747 268 999
Fragments arranged by size:
999 885 747 432 421 268
EcoNI CCTnn'n_nnAGG
Cuts at: 342 342
Size: 3752
EcoO109I rG'GnC_Cy
Cuts at: 392 891 392
Size: 499 3253
EcoRI G'AATT_C
Cuts at: 756 756
Size: 3752
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 1771 1771
Size: 3752
FspI TGC'GCA
Cuts at: 1533 1533
Size: 3752
HaeII r_GCGC'y
Cuts at: 1186 1434 2456 3435 1186
Size: 248 1022 979 1503
Fragments arranged by size:
1503 1022 979 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 229 261 1594 1624 1626 1656 229
Size: 32 1333 30 2 30 2325
Fragments arranged by size:
2325 1333 32 30 30 2
HincII GTy'rAC
Cuts at: 791 791
Size: 3752
KasI G'GCGC_C
Cuts at: 1182 1430 1182
Size: 248 3504
KpnI G_GTAC'C
Cuts at: 772 772
Size: 3752
MluI A'CGCG_T
Cuts at: 3185 3185
Size: 3752
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 2423 2607 2423
Size: 184 3568
MscI TGG'CCA
Cuts at: 1513 1513
Size: 3752
MslI CAynn'nnrTG
Cuts at: 479 583 1741 1868 479
Size: 104 1158 127 2363
Fragments arranged by size:
2363 1158 127 104
MspA1I CmG'CkG
Cuts at: 304 863 1177 1537 2550 2795 304
Size: 559 314 360 1013 245 1261
Fragments arranged by size:
1261 1013 559 360 314 245
NaeI GCC'GGC
Cuts at: 1933 1933
Size: 3752
NarI GG'CG_CC
Cuts at: 1183 1431 1183
Size: 248 3504
NcoI C'CATG_G
Cuts at: 107 107
Size: 3752
NgoMIV G'CCGG_C
Cuts at: 1931 1931
Size: 3752
NspI r_CATG'y
Cuts at: 805 1305 2212 805
Size: 500 907 2345
Fragments arranged by size:
2345 907 500
PciI A'CATG_T
Cuts at: 2208 2208
Size: 3752
PfoI T'CCnGG_A
Cuts at: 653 653
Size: 3752
PmeI GTTT'AAAC
Cuts at: 751 751
Size: 3752
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2891 2923 2891
Size: 32 3720
PpuMI rG'GwC_Cy
Cuts at: 392 392
Size: 3752
PstI C_TGCA'G
Cuts at: 799 1484 799
Size: 685 3067
PvuI CG_AT'CG
Cuts at: 104 104
Size: 3752
PvuII CAG'CTG
Cuts at: 1177 1537 1177
Size: 360 3392
RsrII CG'GwC_CG
Cuts at: 1947 1947
Size: 3752
SacI G_AGCT'C
Cuts at: 766 766
Size: 3752
SacII CC_GC'GG
Cuts at: 305 305
Size: 3752
SalI G'TCGA_C
Cuts at: 789 789
Size: 3752
SapI GCTCTTCn'nnn_
Cuts at: 1775 1985 1775
Size: 210 3542
SbfI CC_TGCA'GG
Cuts at: 799 799
Size: 3752
ScaI AGT'ACT
Cuts at: 3269 3269
Size: 3752
SfcI C'TryA_G
Cuts at: 33 342 795 1480 2473 2664 33
Size: 309 453 685 993 191 1121
Fragments arranged by size:
1121 993 685 453 309 191
SfoI GGC'GCC
Cuts at: 1184 1432 1184
Size: 248 3504
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 3752
SmaI CCC'GGG
Cuts at: 774 774
Size: 3752
SmlI C'TyrA_G
Cuts at: 907 1822 2314 2576 907
Size: 915 492 262 2083
Fragments arranged by size:
2083 915 492 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 3752
SphI G_CATG'C
Cuts at: 805 1305 805
Size: 500 3252
StyI C'CwwG_G
Cuts at: 107 886 1240 107
Size: 779 354 2619
Fragments arranged by size:
2619 779 354
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 1335 1468 1927 2136 1335
Size: 133 459 209 2951
Fragments arranged by size:
2951 459 209 133
TatI w'GTAC_w
Cuts at: 432 551 3267 432
Size: 119 2716 917
Fragments arranged by size:
2716 917 119
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 3269 3301 3269
Size: 32 3720
Tth111I GACn'n_nGTC
Cuts at: 1549 1549
Size: 3752
XbaI T'CTAG_A
Cuts at: 783 783
Size: 3752
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 231 1247 231
Size: 1016 2736
XmaI C'CCGG_G
Cuts at: 772 772
Size: 3752
XmnI GAAnn'nnTTC
Cuts at: 595 595
Size: 3752
Enzymes that do cut:
AccI Acc65I AclI AcuI AflIII AgeI AleI AlwNI
ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII
BbeI BbsI BcgI BciVI BclI BglI BglII BlpI
Bme1580I BmrI BplI BpmI BpuEI BsaI BsaAI BsaBI
BsaHI BsaWI BseRI BseYI BsiEI BsiHKAI Bsp1286I BspEI
BspHI BspMI BsrBI BsrDI BsrFI BssSI BstAPI BstBI
BstXI BstYI BtgI BtgZI BtsI DraI DrdI EaeI
EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI
FalI FspI HaeII Hin4I HincII KasI KpnI MluI
MmeI MscI MslI MspA1I NaeI NarI NcoI NgoMIV
NspI PciI PfoI PmeI PpiI PpuMI PstI PvuI
PvuII RsrII SacI SacII SalI SapI SbfI ScaI
SfcI SfoI SgfI SmaI SmlI SpeI SphI StyI
TaqII TatI TstI Tth111I XbaI XcmI XmaI XmnI
Enzymes that do not cut:
AarI AatII AfeI AflII AhdI AlfI AloI ApaI
AscI AvrII BaeI BbvCI BfrBI BmgBI BmtI Bpu10I
BsaXI BsgI BsiWI BsmI BsmBI BsrGI BssHII BstEII
BstZ17I Bsu36I ClaI CspCI DraIII EcoRV FseI FspAI
HindIII HpaI MfeI NdeI NheI NotI NruI NsiI
PacI PasI PflMI PmlI PshAI PsiI PspOMI PspXI
PsrI SanDI SexAI SfiI SgrAI SnaBI SrfI SspI
StuI SwaI XhoI ZraI