Restriction map for
pF1KE0119
(Circular) (Six-base) MAPSORT of: pF1KE0119.seq Check: 4097 from: 1 to: 4136
pF1KE0119 4136 bp
With 182 enzymes: SgfI *
April 24, 2009 13:10 ..
AccI GT'mk_AC
Cuts at: 252 708 1174 252
Size: 456 466 3214
Fragments arranged by size:
3214 466 456
Acc65I G'GTAC_C
Cuts at: 1152 1152
Size: 4136
AclI AA'CG_TT
Cuts at: 3976 3976
Size: 4136
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 404 1961 2393 3140 3408 404
Size: 1557 432 747 268 1132
Fragments arranged by size:
1557 1132 747 432 268
AflIII A'CryG_T
Cuts at: 582 1488 2592 3569 582
Size: 906 1104 977 1149
Fragments arranged by size:
1149 1104 977 906
AgeI A'CCGG_T
Cuts at: 2524 2524
Size: 4136
AlwNI CAG_nnn'CTG
Cuts at: 1441 3008 1441
Size: 1567 2569
ApaLI G'TGCA_C
Cuts at: 539 2906 539
Size: 2367 1769
ApoI r'AATT_y
Cuts at: 267 275 427 441 612 722 751 956 1140
Size: 8 152 14 171 110 29 205 184
Cuts at: 1140 3489 3745 267
Size: 2349 256 658
Fragments arranged by size:
2349 658 256 205 184 171 152 110
29 14 8
AseI AT'TA_AT
Cuts at: 20 20
Size: 4136
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 4136
AvaI C'yCGr_G
Cuts at: 1156 1156
Size: 4136
BamHI G'GATC_C
Cuts at: 1161 1161
Size: 4136
BanI G'GyrC_C
Cuts at: 1152 1566 1814 1849 1152
Size: 414 248 35 3439
Fragments arranged by size:
3439 414 248 35
BanII G_rGCy'C
Cuts at: 1150 2180 1150
Size: 1030 3106
BbeI G_GCGC'C
Cuts at: 1570 1818 1570
Size: 248 3888
BbsI GAAGACnn'nnnn_
Cuts at: 227 227
Size: 4136
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1021 1055 1021
Size: 34 4102
BciVI GTATCCnnnnn_n'
Cuts at: 2034 2795 4136 2034
Size: 761 1341 2034
Fragments arranged by size:
2034 1341 761
BclI T'GATC_A
Cuts at: 624 1656 624
Size: 1032 3104
BglII A'GATC_T
Cuts at: 1651 3688 1651
Size: 2037 2099
BlpI GC'TnA_GC
Cuts at: 1248 1248
Size: 4136
Bme1580I G_kGCm'C
Cuts at: 543 1761 1854 2910 543
Size: 1218 93 1056 1769
Fragments arranged by size:
1769 1218 1056 93
BmrI ACTGGGnnnn_n'
Cuts at: 1075 1348 1429 1764 3908 1075
Size: 273 81 335 2144 1303
Fragments arranged by size:
2144 1303 335 273 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 271 2503 271
Size: 2232 1904
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 206 206
Size: 4136
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1312 2191 2683 2981 1312
Size: 879 492 298 2467
Fragments arranged by size:
2467 879 492 298
BsaI GGTCTCn'nnnn_
Cuts at: 3840 3840
Size: 4136
BsaAI yAC'GTr
Cuts at: 1489 1489
Size: 4136
BsaBI GATnn'nnATC
Cuts at: 6 1650 3687 6
Size: 1644 2037 455
Fragments arranged by size:
2037 1644 455
BsaHI Gr'CG_yC
Cuts at: 1567 1815 2517 4013 1567
Size: 248 702 1496 1690
Fragments arranged by size:
1690 1496 702 248
BsaWI w'CCGG_w
Cuts at: 1325 1846 2524 2798 2945 3356 1325
Size: 521 678 274 147 411 2105
Fragments arranged by size:
2105 678 521 411 274 147
BseYI C'CCAG_C
Cuts at: 926 1561 2319 2896 3392 926
Size: 635 758 577 496 1670
Fragments arranged by size:
1670 758 635 577 496
BsiEI CG_ry'CG
Cuts at: 104 1678 1724 2932 104
Size: 1574 46 1208 1308
Fragments arranged by size:
1574 1308 1208 46
BsiHKAI G_wGCw'C
Cuts at: 543 1150 1928 2118 2910 543
Size: 607 778 190 792 1769
Fragments arranged by size:
1769 792 778 607 190
Bsp1286I G_dGCh'C
Cuts at: 543 1150 1761 1854 1928 2118 2180 2910 543
Size: 607 611 93 74 190 62 730 1769
Fragments arranged by size:
1769 730 611 607 190 93 74 62
BspEI T'CCGG_A
Cuts at: 3356 3356
Size: 4136
BspHI T'CATG_A
Cuts at: 3312 3312
Size: 4136
BspMI ACCTGCnnnn'nnnn_
Cuts at: 1186 1400 1702 2083 1186
Size: 214 302 381 3239
Fragments arranged by size:
3239 381 302 214
BsrBI CCG'CTC
Cuts at: 50 933 2428 2482 3966 4131 50
Size: 883 1495 54 1484 165 55
Fragments arranged by size:
1495 1484 883 165 55 54
BsrDI GCAATG_nn'
Cuts at: 2048 2048
Size: 4136
BsrFI r'CCGG_y
Cuts at: 2134 2315 2524 2134
Size: 181 209 3746
Fragments arranged by size:
3746 209 181
BssSI C'ACGA_G
Cuts at: 2407 2765 2407
Size: 358 3778
BstAPI GCAn_nnn'nTGC
Cuts at: 1513 1513
Size: 4136
BstBI TT'CG_AA
Cuts at: 2497 2497
Size: 4136
BstYI r'GATC_y
Cuts at: 1 819 1161 1628 1651 1986 2232 3244 3330
Size: 818 342 467 23 335 246 1012 86
Cuts at: 3330 3342 3688 1
Size: 12 346 449
Fragments arranged by size:
1012 818 467 449 346 342 335 246
86 23 12
BstZ17I GTA'TAC
Cuts at: 709 709
Size: 4136
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 2086 2267 2430 2086
Size: 181 163 3792
Fragments arranged by size:
3792 181 163
BtsI GCAGTG_nn'
Cuts at: 1379 1379
Size: 4136
DraI TTT'AAA
Cuts at: 528 813 1135 528
Size: 285 322 3529
Fragments arranged by size:
3529 322 285
DrdI GACnn_nn'nnGTC
Cuts at: 1842 2700 1842
Size: 858 3278
EaeI y'GGCC_r
Cuts at: 1721 1895 2286 2313 3658 1721
Size: 174 391 27 1345 2199
Fragments arranged by size:
2199 1345 391 174 27
EagI C'GGCC_G
Cuts at: 1721 1721
Size: 4136
EarI CTCTTCn'nnn_
Cuts at: 2159 2369 2159
Size: 210 3926
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 2344 2654 2800 3946 2344
Size: 310 146 1146 2534
Fragments arranged by size:
2534 1146 310 146
EcoICRI GAG'CTC
Cuts at: 1148 1148
Size: 4136
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 206 404 1961 2393 3140 3408 206
Size: 198 1557 432 747 268 934
Fragments arranged by size:
1557 934 747 432 268 198
EcoO109I rG'GnC_Cy
Cuts at: 1275 1275
Size: 4136
EcoRI G'AATT_C
Cuts at: 275 1140 275
Size: 865 3271
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2155 2155
Size: 4136
FspI TGC'GCA
Cuts at: 1917 1917
Size: 4136
HaeII r_GCGC'y
Cuts at: 1570 1818 2840 3819 1570
Size: 248 1022 979 1887
Fragments arranged by size:
1887 1022 979 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1978 2008 2010 2040 1978
Size: 30 2 30 4074
Fragments arranged by size:
4074 30 30 2
HincII GTy'rAC
Cuts at: 1175 1175
Size: 4136
KasI G'GCGC_C
Cuts at: 1566 1814 1566
Size: 248 3888
KpnI G_GTAC'C
Cuts at: 1156 1156
Size: 4136
MfeI C'AATT_G
Cuts at: 327 327
Size: 4136
MluI A'CGCG_T
Cuts at: 3569 3569
Size: 4136
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 2807 2991 2807
Size: 184 3952
MscI TGG'CCA
Cuts at: 1897 1897
Size: 4136
MslI CAynn'nnrTG
Cuts at: 112 587 995 2125 2252 112
Size: 475 408 1130 127 1996
Fragments arranged by size:
1996 1130 475 408 127
MspA1I CmG'CkG
Cuts at: 1247 1561 1921 2934 3179 1247
Size: 314 360 1013 245 2204
Fragments arranged by size:
2204 1013 360 314 245
NaeI GCC'GGC
Cuts at: 2317 2317
Size: 4136
NarI GG'CG_CC
Cuts at: 1567 1815 1567
Size: 248 3888
NgoMIV G'CCGG_C
Cuts at: 2315 2315
Size: 4136
NspI r_CATG'y
Cuts at: 586 679 1189 1689 2596 586
Size: 93 510 500 907 2126
Fragments arranged by size:
2126 907 510 500 93
PciI A'CATG_T
Cuts at: 582 2592 582
Size: 2010 2126
PfoI T'CCnGG_A
Cuts at: 450 450
Size: 4136
PmeI GTTT'AAAC
Cuts at: 1135 1135
Size: 4136
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3275 3307 3275
Size: 32 4104
PstI C_TGCA'G
Cuts at: 1183 1868 1183
Size: 685 3451
PvuI CG_AT'CG
Cuts at: 104 104
Size: 4136
PvuII CAG'CTG
Cuts at: 1561 1921 1561
Size: 360 3776
RsrII CG'GwC_CG
Cuts at: 2331 2331
Size: 4136
SacI G_AGCT'C
Cuts at: 1150 1150
Size: 4136
SalI G'TCGA_C
Cuts at: 1173 1173
Size: 4136
SapI GCTCTTCn'nnn_
Cuts at: 2159 2369 2159
Size: 210 3926
SbfI CC_TGCA'GG
Cuts at: 1183 1183
Size: 4136
ScaI AGT'ACT
Cuts at: 417 3653 417
Size: 3236 900
SfcI C'TryA_G
Cuts at: 33 1105 1179 1864 2857 3048 33
Size: 1072 74 685 993 191 1121
Fragments arranged by size:
1121 1072 993 685 191 74
SfoI GGC'GCC
Cuts at: 1568 1816 1568
Size: 248 3888
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 4136
SmaI CCC'GGG
Cuts at: 1158 1158
Size: 4136
SmlI C'TyrA_G
Cuts at: 1291 2206 2698 2960 1291
Size: 915 492 262 2467
Fragments arranged by size:
2467 915 492 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 4136
SphI G_CATG'C
Cuts at: 679 1189 1689 679
Size: 510 500 3126
Fragments arranged by size:
3126 510 500
SspI AAT'ATT
Cuts at: 342 342
Size: 4136
StyI C'CwwG_G
Cuts at: 1270 1624 1270
Size: 354 3782
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 1719 1852 2311 2520 1719
Size: 133 459 209 3335
Fragments arranged by size:
3335 459 209 133
TatI w'GTAC_w
Cuts at: 415 486 3651 415
Size: 71 3165 900
Fragments arranged by size:
3165 900 71
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 3653 3685 3653
Size: 32 4104
Tth111I GACn'n_nGTC
Cuts at: 1933 1933
Size: 4136
XbaI T'CTAG_A
Cuts at: 1167 1167
Size: 4136
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 114 1631 114
Size: 1517 2619
XmaI C'CCGG_G
Cuts at: 1156 1156
Size: 4136
XmnI GAAnn'nnTTC
Cuts at: 956 956
Size: 4136
Enzymes that do cut:
AccI Acc65I AclI AcuI AflIII AgeI AlwNI ApaLI
ApoI AseI AsiSI AvaI BamHI BanI BanII BbeI
BbsI BcgI BciVI BclI BglII BlpI Bme1580I BmrI
BplI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI
BseYI BsiEI BsiHKAI Bsp1286I BspEI BspHI BspMI BsrBI
BsrDI BsrFI BssSI BstAPI BstBI BstYI BstZ17I BtgZI
BtsI DraI DrdI EaeI EagI EarI EciI EcoICRI
Eco57MI EcoO109I EcoRI FalI FspI HaeII Hin4I HincII
KasI KpnI MfeI MluI MmeI MscI MslI MspA1I
NaeI NarI NgoMIV NspI PciI PfoI PmeI PpiI
PstI PvuI PvuII RsrII SacI SalI SapI SbfI
ScaI SfcI SfoI SgfI SmaI SmlI SpeI SphI
SspI StyI TaqII TatI TstI Tth111I XbaI XcmI
XmaI XmnI
Enzymes that do not cut:
AarI AatII AfeI AflII AhdI AleI AlfI AloI
ApaI AscI AvrII BaeI BbvCI BfrBI BglI BmgBI
BmtI Bpu10I BsaXI BseRI BsgI BsiWI BsmI BsmBI
BsrGI BssHII BstEII BstXI Bsu36I BtgI ClaI CspCI
DraIII EcoNI EcoRV FseI FspAI HindIII HpaI NcoI
NdeI NheI NotI NruI NsiI PacI PasI PflMI
PmlI PpuMI PshAI PsiI PspOMI PspXI PsrI SacII
SanDI SexAI SfiI SgrAI SnaBI SrfI StuI SwaI
XhoI ZraI